CN107365718A - Bacillus megaterium MYB3 and its application in straw fermented feed - Google Patents
Bacillus megaterium MYB3 and its application in straw fermented feed Download PDFInfo
- Publication number
- CN107365718A CN107365718A CN201710298708.3A CN201710298708A CN107365718A CN 107365718 A CN107365718 A CN 107365718A CN 201710298708 A CN201710298708 A CN 201710298708A CN 107365718 A CN107365718 A CN 107365718A
- Authority
- CN
- China
- Prior art keywords
- myb3
- bacillus megaterium
- feed
- stalk
- fermented feed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/11—Bacillus megaterium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/42—Cobalamins, i.e. vitamin B12, LLD factor
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fodder In General (AREA)
Abstract
The present invention relates to bacillus megaterium (Bacillus megaterium) MYB3 and its application in straw fermented feed.The deposit number of the bacillus megaterium MYB3 is CGMCC No.13429, and possesses cellulose, hemicellulose degradation ability.During the bacillus megaterium MYB3 can produce feed as microbial bacterial agent added to stalk fermentation, and vitamin B12 can be produced simultaneously.Instant invention overcomes in current various microbiological fermented feed leavenings almost without the deficiency of cellulose, hemicellulose degradation about crucial bacterium, more suitable for the production of straw fermented feed.
Description
Technical field
The invention belongs to field of agricultural microbial technology, is related to bacillus megaterium MYB3 and its in straw fermented feed
Application.
Background technology
China is a large agricultural country, with the raising of crop yield, generates substantial amounts of stalk, except a part is answered
For biogas fermentation, support outside mushroom, the superfluous stalk overwhelming majority is by open incineration.The stalk in China is produced total amount per year and exceeded
As many as 700000000 tons, ranked the first in the world, and contain the abundant nutrient such as nitrogen (N), phosphorus (P), potassium (K) in crop material,
The stalk that stalk accounts for the such flood tides of 12%-19% of China's organic manure resource total amount as organic manure resource does not obtain effective profit
With in field, open incineration causes serious pollution to surrounding environment such as air, soil, wastes the living resources of preciousness.
With animal husbandry, industrial expansion, straw utilization is also increasingly taken seriously.But the Land use systems of China's stalk are also at present
Remain at low levels, still there is very big gap compared with developed countries.
The agricultural crop straw utilization ways in Jilin Province mainly have four kinds, cross abdomen returning to the field, make fertile returning to the field, rural waste,
The raw material of industry, for making industry pulp, building material board etc..What is be wherein widely used is exactly to utilize to burn stalk also
Field.At present, most of resident still makees cooking and house heating energy with agricultural crop straw and vegetation etc. in country in Jilin province.Biomass energy
Resource includes agricultural crop straw, fuel wood and a variety of debirs, is the main energy sources in rural area, and utilization is about more than 5,280 ten thousand tons of marks
Quasi- coal, accounts for 70% of energy consumption or so.At present, biomass energy is mainly used in directly burning, and utilization rate is low.Stalk integrates
Using can not only drive the modernization of agriculture, promote the sustainable development of agricultural production, but also can be
The substantial amounts of cheap raw material of the offers such as herding, edible mushroom, the energy and processing industry, promote it to scale, commercialization, industrialization direction
Development, so as to drive the change of the structure of agricultural production or even whole rural structure, forms a long point of new increasing one of rural economy.Pin
To China's straw utilization present situation, so more to suit measures to local conditions, utilize stalk resource by advanced modern science and technology, stalk is improved
Utilization rate, expand stalk utilization ways, increase stalk added value, so as to effectively meet society and expanding economy need
Ask.
Stalk softening mode can not only be improved by carrying out stalk softening using microorganism formulation, and can obtain higher economy
It benefit, can also reach effect free from environmental pollution, to keep ecological balance, meet the principle of sustainable development.At present, sold in market
The relevant stalk fermentation microorganism formulation sold is a lot, it is most of be all with lactic acid bacteria, hay bacillus, it is compound micro- based on saccharomycete
Biology, or the compost microorganism formulation of mould, other bacterium with cellulose degradation ability as stover ferment agent, it is few
For cellulose in stalk, hemicellulose degradation capability and to be fermented into the bacillus megaterium of straw feed be principal leavening agent
Elaboration.
Microbe species in microorganism formulation are more, great variety of goods, still, but current market sales of overwhelming majority
Stalk fermentation microbial inoculum, such as lactic acid bacteria, saccharomycete, hay bacillus etc., opened around the keeping quality of stalk and palatability etc.
The product issued, ensiling and the theory of yellow storage (microbial) etc. and concept, the firm method of the quality of ensiling corresponding to stalk etc..
In the stalk fermentations such as Huang storage, microorganism formulation lacks decomposition and inversion effect to cellulose and hemicellulose etc., slightly raw using this
Thing formulation products carry out stalk fermentation, have impact on the conversion ratio of stalk useful component.
The content of the invention
The present invention be in view of the above-mentioned problems in the prior art and make, it is an object of the invention to provide one kind
Bacillus megaterium MYB3 and its application in straw fermented feed, the bacillus megaterium MYB3 possess cellulose, half fibre
Tie up plain degradation capability.
To achieve the above object, bacillus megaterium of the invention (Bacillus megaterium strain) MYB3,
China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), preservation are preserved on December 07th, 2016
Numbering CGMCC No.13429, preservation address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Chinese Academy of Sciences's microbe research
Institute, and the bacillus megaterium MYB3 possesses cellulose, hemicellulose degradation ability.
Further, bacillus megaterium MYB3 of the invention growth pH value is 3-12.
Further, bacillus megaterium MYB3 of the invention growth temperature is 20-45 DEG C.
In order to realize above-mentioned another object, applications of the bacillus megaterium MYB3 of the present invention in straw fermented feed is led to
Such technical scheme is crossed to realize:The bacillus megaterium MYB3 produces feed as microbial bacterial agent added to stalk fermentation
During.
Further, the application of bacillus megaterium MYB3 of the invention in straw fermented feed, sent out in the stalk
Vitamin B12 is produced during ferment production feed simultaneously.
Beneficial effect：
1. bacillus megaterium (Bacillus megaterium strain) MYB3 of the present invention has highly effective cellulose
With hemicellulose degradation ability, difference and other microbial fermentation preparations, stalk fermentation microbial bacterial agent is suitable as, makes microbial
Feed, particularly yellow storage feed.
2. bacillus megaterium (Bacillus megaterium strain) MYB3 resistance to acids and bases scopes of the present invention are wide,
The pH scopes of growth are 3-12.
3. bacillus megaterium (Bacillus megaterium strain) MYB3 bacterial strains of the present invention are at 20-45 DEG C
Culture 24 hours survival thalline number be 6.34-6.61 (Log, CFU/mL), 35 DEG C cultivate under in 24 hours thalline survival
Thalline number reach 6.8 (Log, CFU/mL), be adapted to different temperatures in the range of ferment, be adapted to the temperature conditionss in animal intestinal tract
Lower existence.
4. vitamin B12 can be produced during stalk fermentation produces feed, Jia Weisheng is not had to or lacks as feed
Plain B12 additives, feed ingredient is saved, improve feed nutritive value.
Brief description of the drawings
To be illustrated more clearly that the embodiment of the present invention, below to being used in the description of specific embodiment part
To accompanying drawing be briefly described.
Description of reference numerals is as follows：
Fig. 1 is isolated bacillus megaterium (the Bacillus megaterium of the first embodiment of the present invention
Strain the photo of micro- Microscopic observation after) colonial morphologies and Gram of the MYB3 on LB culture mediums dye；
Fig. 2 is the isolated huge gemma of the isolated first embodiment of the present invention of the first embodiment of the present invention
Bacillus (Bacillus megaterium strain) MYB3 16SrDNA analysis results；
Fig. 3 is bacillus megaterium (Bacillus megaterium strain) MYB3 of the second embodiment of the present invention
Thalline survival number at different temperatures；
Fig. 4 is bacillus megaterium (Bacillus megaterium strain) MYB3 of the second embodiment of the present invention
Thalline survival number under condition of different pH；
Fig. 5 is bacillus megaterium (Bacillus megaterium strain) MYB3 of the third embodiment of the present invention
Cellulose, the change of hemicellulose resolution ratio during stalk fermentation produces feed；
Fig. 6 is the changes of contents of vitamin B12 in third embodiment of the present invention straw fermented feed；
Fig. 7 is the activity change of catabolic enzyme in fourth embodiment of the present invention stalk fermentation；
Fig. 8 is the cellulose decomposition rate of fifth embodiment of the present invention different experiments packet；
Fig. 9 is the hemicellulose resolution ratio of fifth embodiment of the present invention different experiments packet.
Embodiment
To make the object, technical solutions and advantages of the present invention clearer, below, with reference to accompanying drawing to the specific of the present invention
Embodiment is described in detail.
The separation and identification of first embodiment strain
The fertile soil around the rotten wood and rotten wood in Yanji cap mountain is gathered as sample, each sample 5g, each sample
Middle addition 195mL sterilized waters, under 120rpm rotating speeds vibration shake up 30min, after standing, take 2mL supernatants, be inoculated into containing
In the 300mL triangular flasks of 100mL enriched mediums (containing filter paper bar), broken section of pretreated maize straw is added in triangular flask
3g (Huang storage forage standard 2-2.5cm), 30 DEG C, cultivate under 120rpm rotating speeds.There is yellow flocculation in filter paper disintegration, sample liquid after 5d
Material, i.e. Straw decomposing shape.By sample inoculation into the enriched medium required on an equal basis squamous subculture, so circulation ten times, it is right
Straw decomposing bacterium is stabilized, and the bacterium after taming is inoculated into LB fluid nutrient mediums and cultivated, by the bacterium on solid medium after 24h
Strain screening, isolates different strains one by one, in being circulated 5 times on LB solid mediums, after in the culture of strain without miscellaneous bacteria, sieves
Select bacterial strain MYB3.As shown in Figure 1-2, through the colonial morphology on LB culture mediums and Gram dyeing after micro- Microscopic observation and
16SrDNA identification and analysis, bacterial strain MYB3 are accredited as bacillus megaterium, and the bacillus megaterium MYB3 was on December 07th, 2016
It is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), deposit number CGMCC
No.13429, preservation address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica.
Enriched medium:K2HPO42g, (NH4)2SO41.4g, MgSO4·7H2O 0.3g, CoCl22mg, CaCl2
0.3g, FeSO4·7H2O 5mg,ZnSO41.7mg, MnSO4·H2O 1.6mg, filter paper (qualitative filter paper), distilled water 1L.
LB culture mediums:Yeast extract 5g, peptone 10g, NaCl 10g, distilled water 1L.
Growth characteristics research under the different condition of second embodiment strain
The number changes of bacterial strain MYB3 at different temperatures：0.1mL bacterium solution to be measured is accurately drawn in aseptic operating platform
After access sterilizing in the centrifuge tube equipped with 0.9mL distilled water, this centrifuge tube is to dilute the bacterium solution after 10 times.Again from 10 times of dilution
Centrifuge tube in the accurate 0.1mL that draws access in next centrifuge tube equipped with 0.9mL distilled water, the as bacterium of 100 times of dilution
Liquid, dilution 10 is obtained after being continued until access according to the method described above8Bacterium solution again.The bacterium solution 1mL of different dilution factors is drawn respectively
It is equipped with after being put into sterilizing on the culture dish of LB solid mediums, carries out colony counting method experiment.By culture dish be respectively put into 20 DEG C,
30 DEG C, 35 DEG C, 40 DEG C, the clump count calculated after 24h in culture dish is cultivated in 45 DEG C of incubator.
Number changes of the bacterial strain MYB3 under condition of different pH：By inoculation in solid medium to LB fluid nutrient mediums
In, 30 DEG C, absorption 0.1mL is transferred to the 10mL phosphate that pH value is 2,3,4,5,6,7,8,9,10,11,12 respectively after cultivating 1d
In cushioning liquid, 4h is cultivated in 30 DEG C, by the bacterium solution dilution 10 under every kind of pH value condition-1-10-8Carry out flat board coating respectively again,
Colony counting method experimental calculation viable count is carried out after 30 DEG C of culture 24h.
As shown in figure 3, the thalline number that bacterial strain MYB3 cultivates survival in 24 hours at 20 DEG C is 6.34 (Log, CFU/mL),
The thalline number that survival in 24 hours is cultivated at 45 DEG C is 6.61 (Log, CFU/mL).Bacterial strain viable count highest under conditions of 35 DEG C,
It is that MYB3 bacterial strains optimum growth temperature is 35 DEG C that thalline number, which reaches 6.8 (Log, CFU/mL),.The scope of test is 20-45 DEG C, full
The condition of temperature, also is adapted for the temperature survived in animal intestinal tract during foot general stalk fermentation.
As shown in figure 4, MYB3 bacterial strains are that 3-12 can survive in pH scopes.In pH=3 viable count be 2.48 (Log,
CFU/mL), grown rapidly as pH=6, viable count is 3.87 (Log, CFU/mL), and the growth in pH=8 is the most vigorous, living
Bacterium number reaches 5.4 (Log, CFU/mL), is begun to decline afterwards with pH rise viable count, in pH=12, viable count is reduced to
4.39 (Log, CFU/mL).
Applications of the 3rd embodiment bacterial strain MYB3 in stalk fermentation produces feed
It is 2-5cm or so that stalk (preferably maize straw), which is minced to length, and water is adjusted using distilled water (or underground water)
It is divided into 70%, while the acid-base value of stalk is adjusted to pH=3 using lactic acid, sealing is placed 1-2 days at room temperature, and sterilization (is adopted
Cultivated after sample in LB solid mediums, do not observe bacterium).It is after sterilization that bacterial strain MYB3 isolated in first embodiment is equal
The microbial bacterial agent as stalk fermentation in stalk (preferably maize straw) is inoculated into evenly, is trained after pack sealing in 30 DEG C of constant temperature
Support and fermented in case.Bacterial strain MYB3 viable count remains to 6.8 × 10 after fermenting 10 days5cfu/mL。
As shown in figure 5, after stalk (preferably maize straw) ferments 10 days, cellulose and hemicellulose resolution ratio respectively reach
41% and 28%.Stalk pH=4.8 after fermentation, reach the requirement of yellow storage feed.
Vitamin B12 in sampling analysis fermentate, such as schemes when stalk (preferably maize straw) ferments 3,5,10,20 days
Shown in 6, its content increases as fermentation time is increased, and concentration reaches 13.5mg/L when 20 days.
The measure of enzymatic activity is decomposed during fourth embodiment stalk liquid fermentation
Cellulase activity determines：The bacterium solution 0.5mL after fermentation is taken, the CMC solution that 1mL mass fractions are 1% is added and (uses
PH=4.8 0.1mol/L HAC-NaAC is prepared), 50 DEG C of insulation 30min add 3mL DNS reagents, boiling water bath 5min, cold
But 25mL is diluted to afterwards, and reduced sugar is surveyed under 540nm absorbances.
Hemicellulase activity determines：The bacterium solution 0.5mL after appropriate fermentation is taken, the 1.0g/100mL wood for adding 1.5mL is poly-
Sugar juice (is prepared) with pH=4.8 0.1mol/L HAC-NaAC, 50 DEG C of insulation 30min, adds 3mL DNS reagents, boiling water
5min is bathed, 25mL is diluted to after cooling, reduced sugar is surveyed under 520nm absorbances.
Amylase activity determines：The bacterium solution 0.5mL after fermentation is taken, adds 0.5mL 0.1mol/l citrate buffer solutions (pH
For 5.6), 40 DEG C of water-baths are incubated 15min, 1% soluble starch preheated, 40 DEG C of water-bath insulations are added at 1mL40 DEG C
0.4mol/L NaOH 2mL are added after 5min immediately, terminate the activity of enzyme.Above-mentioned solution 2mL is taken to be fitted into 25mL volumetric flask,
3mL DNS reagents are added, boiling water bath 5min, 25mL are diluted to after cooling, reduced sugar is surveyed under 540nm absorbances.
As shown in fig. 7, it is in invertedU curve state that enzymatic activity is overall.The enzymatic activity of cellulolytic enzyme is after fermenting 1 day
4.672 (U/mL), gradually rise afterwards, are to reach peak at the 3rd day, and enzymatic activity is 19.279 (U/mL), continue point with stalk
Solution, activity are begun to decline, and at the 5th day, activity only had 5.818 (U/mL).The enzyme activity of hemicellulase catabolic enzyme after fermenting 1 day
Property is 3.95 (U/mL), gradually rises afterwards, was to reach peak at the 2nd day, enzymatic activity is 6.668 (U/mL), is continued with stalk
Decompose, activity is begun to decline, and at the 5th day, activity only had 4.65 (U/mL).The enzymatic activity of amylase is 4.1 (U/ after fermenting 1 day
ML), gradually rising afterwards, peak was reached at the 3rd day, enzymatic activity reaches 9.844 (U/mL), and subsequent activity is begun to decline,
Activity is 7.045 (U/mL) at the 5th day.
5th embodiment straw fermented feed characteristic
It is different with corn flour to maize straw as microbial bacterial agent using the isolated MYB3 bacterial strains of first embodiment
The mixture of ratio is fermented, and control group adds water using maize straw, and cellulose and hemicellulose are measured respectively after 3d and 5d
Content.
As shown in figure 8, at the 15th day, maize straw (30:0) cellulose decomposition rate reaches 43.18%, maize straw
With corn powder mixture (30:6) the cellulose decomposition rate of relative comparison group (stalk adds water) reaches 77.26% in, experimental result
Show the resolution ratio for adding corn flour to increase cellulose in stalk fermentation.
As shown in figure 9, at the 15th day, maize straw (30:0) hemicellulose resolution ratio reaches 27.91%, corn stalk
Stalk and corn powder mixture (30:6) the hemicellulose resolution ratio of relative comparison group (stalk adds water) reaches 32.58% in, experiment
As a result show plus corn flour can increase the resolution ratio of hemicellulose in stalk fermentation, but trend more increased than cellulose decomposition rate
It is not very big.
The present invention bacillus megaterium (Bacillus megaterium strain) MYB3 have highly effective cellulose and
Hemicellulose degradation ability, difference and other microbial fermentation preparations, are suitable as stalk fermentation microbial bacterial agent, make microbial and raise
Material, particularly yellow storage feed；Bacillus megaterium (Bacillus megaterium strain) MYB3 acid and alkali-resistances of the present invention
Property scope is wide, and the pH scopes of growth are 3-12；The bacillus megaterium (Bacillus megaterium strain) of the present invention
The thalline number that MYB3 bacterial strains cultivate survival in 24 hours at 20-45 DEG C is 6.34-6.61 (Log, CFU/mL), under 35 DEG C of cultures
The thalline number of the survival of thalline in 24h reaches 6.8 (Log, CFU/mL), is adapted to ferment in the range of different temperatures, is adapted in animal
Survived under temperature conditionss in enteron aisle；Vitamin B12 can be produced during stalk fermentation produces feed, as feed not
With or less plus vitamin B12 additive, save feed ingredient, improve feed nutritive value.
Embodiment as described above is only used for illustrating the spirit of the present invention, and protection scope of the present invention is not limited to
This, to those of ordinary skill in the art, can pass through change according to technology contents disclosed in this specification certainly
More, the mode of displacement or modification makes other embodiments easily, and these other embodiments should all cover in the present invention
Protection domain within.
Claims (7)
- A kind of 1. bacillus megaterium MYB3, it is characterised in that the bacillus megaterium (Bacillus megaterium) MYB3 deposit number is CGMCC No.13429, and the bacillus megaterium MYB3 possesses cellulose, hemicellulose degradation Ability.
- 2. bacillus megaterium MYB3 according to claim 1, it is characterised in that the life of the bacillus megaterium MYB3 Long pH value is 3-12.
- 3. bacillus megaterium MYB3 according to claim 1 or 2, it is characterised in that the bacillus megaterium MYB3 Growth temperature be 20-45 DEG C.
- 4. applications of any described bacillus megaterium MYB3 in straw fermented feed, its feature exist in claim 1-2 During the bacillus megaterium MYB3 produces feed as microbial bacterial agent added to stalk fermentation.
- 5. applications of the bacillus megaterium MYB3 in straw fermented feed described in claim 3, it is characterised in that described huge During Bacterium anthracoides MYB3 produces feed as microbial bacterial agent added to stalk fermentation.
- 6. applications of the bacillus megaterium MYB3 according to claim 4 in straw fermented feed, it is characterised in that Vitamin B12 is produced during the stalk fermentation production feed simultaneously.
- 7. applications of the bacillus megaterium MYB3 according to claim 5 in straw fermented feed, it is characterised in that Vitamin B12 is produced during the stalk fermentation production feed simultaneously.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710298708.3A CN107365718B (en) | 2017-04-27 | 2017-04-27 | Bacillus megaterium MYB3 and application thereof in straw fermented feed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710298708.3A CN107365718B (en) | 2017-04-27 | 2017-04-27 | Bacillus megaterium MYB3 and application thereof in straw fermented feed |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107365718A true CN107365718A (en) | 2017-11-21 |
| CN107365718B CN107365718B (en) | 2021-02-02 |
Family
ID=60304353
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710298708.3A Active CN107365718B (en) | 2017-04-27 | 2017-04-27 | Bacillus megaterium MYB3 and application thereof in straw fermented feed |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107365718B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109797119A (en) * | 2019-03-11 | 2019-05-24 | 大连大学 | A kind of composite bacteria agent of degrading straw cellulose |
| CN111621433A (en) * | 2020-04-10 | 2020-09-04 | 四川轻化工大学 | Bacillus megaterium for producing cellulase, preparation method and application |
| CN112322549A (en) * | 2020-11-24 | 2021-02-05 | 江南大学 | Strain capable of efficiently converting vitamin D3 into active form and application thereof |
| CN114158646A (en) * | 2021-12-17 | 2022-03-11 | 延边大学 | Complete mixed fermented feed capable of regulating and controlling pH value and preparation method thereof |
| CN114907152A (en) * | 2022-06-23 | 2022-08-16 | 河北冀微生物技术有限公司 | Application of bacillus megatherium RL-126 strain in promoting decomposition of crop straws |
| WO2023108644A1 (en) * | 2021-12-17 | 2023-06-22 | 延边大学 | Total mixed fermentation feed having regulated ph value and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1636066A (en) * | 2001-08-22 | 2005-07-06 | 巴斯福股份公司 | Method for producing vitamin B12 |
| CN1759175A (en) * | 2003-01-11 | 2006-04-12 | 巴斯福股份公司 | Improved method for the production of vitamin B12 |
| EP1719518A1 (en) * | 1998-08-07 | 2006-11-08 | Ganeden Biotech, Inc. | Compositions comprising bacillus coagulans for increasing the solubility and bioavailability of nutritional minerals |
| CN102732463A (en) * | 2012-06-27 | 2012-10-17 | 重庆市瑞宝农业产业集团有限公司 | Bacillus subtilis RB and application thereof |
| CN104789492A (en) * | 2015-03-24 | 2015-07-22 | 福建省农业科学院农业工程技术研究所 | Bacillus megaterium strain and application thereof |
-
2017
- 2017-04-27 CN CN201710298708.3A patent/CN107365718B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1719518A1 (en) * | 1998-08-07 | 2006-11-08 | Ganeden Biotech, Inc. | Compositions comprising bacillus coagulans for increasing the solubility and bioavailability of nutritional minerals |
| CN1636066A (en) * | 2001-08-22 | 2005-07-06 | 巴斯福股份公司 | Method for producing vitamin B12 |
| CN1759175A (en) * | 2003-01-11 | 2006-04-12 | 巴斯福股份公司 | Improved method for the production of vitamin B12 |
| CN102732463A (en) * | 2012-06-27 | 2012-10-17 | 重庆市瑞宝农业产业集团有限公司 | Bacillus subtilis RB and application thereof |
| CN104789492A (en) * | 2015-03-24 | 2015-07-22 | 福建省农业科学院农业工程技术研究所 | Bacillus megaterium strain and application thereof |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109797119A (en) * | 2019-03-11 | 2019-05-24 | 大连大学 | A kind of composite bacteria agent of degrading straw cellulose |
| CN111621433A (en) * | 2020-04-10 | 2020-09-04 | 四川轻化工大学 | Bacillus megaterium for producing cellulase, preparation method and application |
| CN112322549A (en) * | 2020-11-24 | 2021-02-05 | 江南大学 | Strain capable of efficiently converting vitamin D3 into active form and application thereof |
| CN114158646A (en) * | 2021-12-17 | 2022-03-11 | 延边大学 | Complete mixed fermented feed capable of regulating and controlling pH value and preparation method thereof |
| WO2023108644A1 (en) * | 2021-12-17 | 2023-06-22 | 延边大学 | Total mixed fermentation feed having regulated ph value and preparation method thereof |
| CN114907152A (en) * | 2022-06-23 | 2022-08-16 | 河北冀微生物技术有限公司 | Application of bacillus megatherium RL-126 strain in promoting decomposition of crop straws |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107365718B (en) | 2021-02-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105567612B (en) | A kind of degradation composite bacteria agent preparation of garden waste and application | |
| CN107365718A (en) | Bacillus megaterium MYB3 and its application in straw fermented feed | |
| CN102690755B (en) | Compound microbial bacterial preparation for degrading crop straw and preparation method and application of compound microbial bacterial preparation | |
| CN101759494B (en) | Diseases-prevention organic fertilizer prepared by edible fungi residue | |
| CN102442849A (en) | Disease-preventing bio-organic fertilizer prepared from straw and its preparation method | |
| CN101838613A (en) | Agricultural straw fast-degradation decomposition agent strain composition | |
| CN103602592B (en) | Cellulose-degradation fungus and preparation of inoculum and application thereof | |
| CN103484396B (en) | New strain of streptomyces thermocarboxydus and application thereof | |
| CN102533553B (en) | Organic matter decomposition combined fungicide for rapidly degrading rice straws and application method thereof | |
| CN103087973A (en) | Screening and domesticating method of composite microbial system for low-temperature degradation of straws | |
| CN105524858A (en) | High-temperature-resistant decomposing microbial inoculum of organic wastes and application of high-temperature-resistant decomposing microbial inoculum | |
| CN104692844A (en) | Preparation method of bioorganic fertilizer | |
| CN101759493A (en) | Biological disease-preventing organic fertilizer and preparation method thereof | |
| CN103159570A (en) | Solid microbial fertilizer and preparation method thereof | |
| CN108203352A (en) | A kind of production method of kitchen garbage organic fertilizer | |
| CN101933439A (en) | Method for improving phellinus igniarius hypha amount of submerged culture by utilizing plant oil | |
| CN103361281B (en) | High-temperature degrading bacteria and application thereof | |
| CN105754888A (en) | Bacillus licheniformis and microbial agent and their application in fermentation bed culture | |
| CN103074228B (en) | Composite fermentation inoculant for processing animal wastes, and preparation method and application thereof | |
| CN109439585A (en) | One plant of adipic acid kelvin bacterium and its application in cellulose degradation | |
| CN105950483A (en) | Candida glabrata and application in kitchen waste | |
| CN103409383A (en) | Method used for accelerating lignin degradation in Aspergillus oryzae solid state fermentation | |
| CN104860401A (en) | Application of Rhizopus oryzae strain JHSW01 to the fermentation of organic waste liquid and forestry and agricultural waste | |
| CN104560817A (en) | Thermophilic bacillus licheniformis UTM102 for producing phytase and application of thermophilic bacillus licheniformis UTM102 | |
| CN101248833B (en) | Method for producing food and rink fag-end solid-state fermentation protein feed stuff, composite zymocyte liquid and preparing method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |