CN104692844A - Preparation method of bioorganic fertilizer - Google Patents
Preparation method of bioorganic fertilizer Download PDFInfo
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- CN104692844A CN104692844A CN201310665870.6A CN201310665870A CN104692844A CN 104692844 A CN104692844 A CN 104692844A CN 201310665870 A CN201310665870 A CN 201310665870A CN 104692844 A CN104692844 A CN 104692844A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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Abstract
The invention discloses a preparation method of a bioorganic fertilizer. The method is characterized by adopting a complex microorganism bacterium agent formed by bacillus subtilis, aspergillus oryzae, trichoderma reesei, phanerochaete chrysosporium and geobacillus stearothermophilus as a decomposition agent to degrade straws to prepare the bioorganic fertilizer. The preparation method has the beneficial effects that the strain composition of the complex microorganism bacterium agent is new flora composition, which takes full advantage of the physiological activities of strains to generate cellulose, hemicellulose and biological enzymes, such as ligninase, to accelerate the decomposition process of straws, thus preparing the bioorganic fertilizer; the organic straw fertilizer prepared by the preparation method is used as a base fertilizer, so that the contents of available nitrogen, rapidly available phosphorus, potassium and organic matters in soil are higher than those of available nitrogen, rapidly available phosphorus, potassium and organic matters in the soil in which straws are directly used and fertilizers are not applied; the urease activity and cellulase activity of the soil are obviously improved compared with those of the soil in which straws are directly used and fertilizers are not applied.
Description
Technical field
The present invention relates to a kind of organic fertilizer, especially relate to a kind of preparation method of bio-organic fertilizer.
Background technology
Agricultural crop straw is a kind of huge resource, has that quantity is large, kind is many, widespread feature.According to statistics, China's stalk annual production is about about 700,000,000 tons, but straw utilization efficiency is extremely low, and most of stalk is wantonly burned, puts aside as useless, causes serious environmental pollution and the wasting of resources.Current stalk mostly is direct returning to farmland at Land use systems agriculturally, but because cold ground soil straw-returning decomposition rate is slow and cause the soil moisture to reduce, affects plant growth, so cause the ratio of straw-returning always lower.The optimal path that straw-returning addresses these problems beyond doubt is realized by compost.Volume and weight that germ in refuse and grass seeds, reduction store up can be killed by composting, be conducive to storage and use.Not only can solve the problem of environmental pollution of large-scale cultivation factory and burning straw generation, and to developing fertilizer, keeping and increase soil fertility, promote that agricultural sustainable development is significant.
Straw compost is as the effective way of a kind of solid waste minimizing, resource utilization, and its digest process carries out in the dynamic process of biological community structure succession.But Natural compost required time is long, and straw utilization rate is lower, is therefore badly in need of a kind of efficient complex microbial inoculum of development and improves the speed of becoming thoroughly decomposed of stalk and rate of becoming thoroughly decomposed.
Summary of the invention
The invention provides a kind of preparation method of bio-organic fertilizer, bio-organic fertilizer of the present invention take stalk as composting material, be applied with the complex microbial inoculum being beneficial to the ingredient degradation such as Mierocrystalline cellulose, hemicellulose, xylogen, accelerate Straw decomposing, prepare straw organic fertilizer, apply in farmland, improve soil property, improve crop yield and quality.
Technical scheme of the present invention is as follows:
A preparation method for bio-organic fertilizer, comprises the steps:
(1) preparation of complex micro organism fungicide:
The active ingredient of described complex micro organism fungicide is subtilis (Bacillus subtilis), aspergillus oryzae (Aspergillus niger), Trichodermareesei (Trichoderma viride), whiterot fungi (Phanerochaetechrysosporium), Geobacillus stearothermophilus (Geobacillus stearothermophilus), and described complex micro organism fungicide prepares with the following method:
1. actication of culture: each bacterial classification described above is inoculated on slant medium respectively; Subtilis 28 ~ 32 DEG C, pH is 7.2 ~ 7.4, and incubation time is 1 ~ 3 day; Aspergillus oryzae culture temperature 25 DEG C, incubation time is 4 ~ 7 days; Trichodermareesei culture temperature 25 ~ 28 DEG C, natural PH, incubation time is 4 ~ 7 days; Whiterot fungi culture temperature 25 ~ 28 DEG C, incubation time is 4 ~ 7 days; Geobacillus stearothermophilus culture temperature 60 DEG C, PH6.8, incubation time is 1 ~ 3 day;
2. first class inoculum is cultivated: be inoculated in respectively by each bacterial classification that step (1) activates in liquid nutrient medium corresponding to each bacterial classification, shaking table shaking culture, until bacterial classification living bacteria count is respectively 10 in each bacteria culture fluid
6-10
8individual/ml;
3. second order fermentation: step (2) each bacteria culture fluid is inoculated in strain fermentation substratum respectively by inoculum size 1-10%, shaking table shaking culture, until bacterial classification living bacteria count is respectively 10 in each strain fermentation nutrient solution
6-10
8individual/mL, collect fermentation culture, respectively each strain fermentating liquid;
4. each strain fermentating liquid that step (3) is obtained is mixed, obtain mixed bacteria liquid; In mixed bacteria liquid, fermentation of bacillus subtilis nutrient solution accounts for 15 ~ 20wt%, aspergillus oryzae fermentation culture accounts for 15 ~ 20wt%, and Trichodermareesei fermentation culture accounts for 30 ~ 40wt%, whiterot fungi fermentation culture accounts for 10 ~ 15wt%, Geobacillus stearothermophilus fermentation culture accounts for 10 ~ 20wt%;
5. three grade fermemtation: be inoculated in composite fungus agent substratum by inoculum size 1 ~ 5% by mixed bacteria liquid, cultivates 4 ~ 7 days, makes bacterium number in fermented liquid reach 10 at 30 DEG C
6~ 10
8individual/mL, obtains complex micro organism fungicide;
(2) preparation of bio-organic fertilizer
Stalk pulverizer is ground into the segment being less than 2cm, adds the complex micro organism fungicide of step (1) in the ratio of 1 ~ 8kg complex micro organism fungicide/ton stalk, 25 ~ 30 DEG C of fermentations 15 ~ 40 days.
In preferred technical scheme, in the step (2) of technique scheme: stalk pulverizer is ground into the segment being less than 2cm, urea is added in the ratio of 1 ~ 10kg urea/ton stalk, animal excrement are added in the ratio of 10 ~ 100kg animal excrement/ton stalk, the complex micro organism fungicide of step (1) is added, 25 ~ 30 DEG C of fermentations 15 ~ 40 days in the ratio of 1 ~ 8kg complex micro organism fungicide/ton stalk.
The invention has the beneficial effects as follows: the bacterial classification assembly of complex micro organism fungicide of the present invention is a kind of new flora assembly, make full use of the physiological activity of bacterial classification, produce the biological enzymes such as Mierocrystalline cellulose, hemicellulose and lignoenzyme, accelerate the maturity of stalk, thus prepare bio-organic fertilizer.Straw organic fertilizer prepared by the present invention is used as base manure, soil alkaline hydrolysis nitrogen, rapid available phosphorus, potassium and organic content are all high than directly using the soil content of stalk and non-applying fertilizer, and soil urease liveness and cellulase activity are also all than directly using the soil of stalk and non-applying fertilizer to significantly improve.
Embodiment
Following non-limiting example can make the present invention of those of ordinary skill in the art's comprehend, but does not limit the present invention in any way.Test method described in embodiment, if no special instructions, is ordinary method; Described reagent and material, if no special instructions, all can obtain from commercial channels, or can prepare in conventional manner.
Embodiment 1
A preparation method for bio-organic fertilizer, comprises the steps:
(1) preparation of complex micro organism fungicide:
The active ingredient of described complex micro organism fungicide is subtilis (Bacillus subtilis), aspergillus oryzae (Aspergillus niger), Trichodermareesei (Trichoderma viride), whiterot fungi (Phanerochaetechrysosporium), Geobacillus stearothermophilus (Geobacillus stearothermophilus), and described complex micro organism fungicide prepares with the following method:
1. actication of culture: each strain inoculation described above is on the slant medium that each bacterial classification is corresponding; Subtilis 28 ~ 32 DEG C, pH is 7.2 ~ 7.4, and incubation time is 2 days; Aspergillus oryzae culture temperature 25 DEG C, incubation time is 7 days; Trichodermareesei culture temperature 25 ~ 28 DEG C, natural PH, incubation time is 5 days; Whiterot fungi culture temperature 25 ~ 28 DEG C, incubation time is 6 days; Geobacillus stearothermophilus culture temperature 60 DEG C, PH6.8, incubation time is 3 days;
2. first class inoculum is cultivated: be inoculated in respectively by each bacterial classification that step (1) activates in liquid nutrient medium corresponding to each bacterial classification, shaking table shaking culture, until bacterial classification living bacteria count is respectively 10 in each bacteria culture fluid
7individual/ml;
3. second order fermentation: step (2) each bacteria culture fluid is inoculated in strain fermentation substratum respectively by inoculum size 5%, shaking table shaking culture, until bacterial classification living bacteria count is respectively 10 in each strain fermentation nutrient solution
7individual/mL, collect fermentation culture, respectively each strain fermentating liquid;
4. each strain fermentating liquid that step (3) is obtained is mixed, obtain mixed bacteria liquid; In mixed bacteria liquid, fermentation of bacillus subtilis nutrient solution accounts for 15wt%, aspergillus oryzae fermentation culture accounts for 15wt%, and Trichodermareesei fermentation culture accounts for 30wt%, whiterot fungi fermentation culture accounts for 10wt%, Geobacillus stearothermophilus fermentation culture accounts for 10wt%;
5. three grade fermemtation: be inoculated in composite fungus agent substratum by inoculum size 2% by mixed bacteria liquid, cultivates 4 ~ 7 days, makes bacterium number in fermented liquid reach 10 at 30 DEG C
7individual/mL, obtains complex micro organism fungicide.
Described strain fermentation substratum is: the straw 13.4g/L of explosion, peptone 11g/L, ammonium sulfate 13g/L, glucose 6g/L, potassium primary phosphate 10.5g/L.
The liquid nutrient medium of described each bacterial classification is:
The liquid culture based formulas that subtilis adopts is: plain agar: beef infusion broth 1.0L, peptone 10g, NaCl5.0g, agar 20.0-20.5g, PH7.2-7.4, the preparation of beef infusion broth: lean beef is cleaned, chopping, takes 550g and is soaked in 1375ml water, soak a night, filter, after filtering packing, 0.6kgf/cm240min is for subsequent use;
The liquid culture based formulas that aspergillus oryzae adopts is: 5
0be wort 1.0L, agar 15.0g, natural PH;
The liquid culture based formulas that Trichodermareesei adopts is: PDA substratum, removes the potato 200 grams of skin, is cut into small pieces, add water 1000 milliliters and boil 30 minutes elimination potato balls, filtrate is complemented to 1000 milliliters, add glucose 20 grams, 15 grams, agar, dissolves rear packing, 15 pounds of sterilizings 30 minutes.Nature PH;
The liquid culture based formulas of whiterot fungi is: PDA substratum, removes the potato 200 grams of skin, is cut into small pieces, add water 1000 milliliters and boil 30 minutes elimination potato balls, filtrate is complemented to 1000 milliliters, add glucose 20 grams, 15 grams, agar, dissolves rear packing, 15 pounds of sterilizings 30 minutes.Nature PH;
The liquid culture based formulas that Geobacillus stearothermophilus adopts is: nutrient agar, glucose 100g, yeast powder 10.0g, CaCO
320.0g, agar 15.0g, distilled water 1.0L, PH6.8.
Described composite fungus agent culture medium prescription is: the straw of explosion is 13.4g/L, peptone 11g/L, ammonium sulfate 13g/L, glucose 6g/L, potassium primary phosphate 10.5g/L.
(2) preparation of bio-organic fertilizer
Pulverized by maize straw pulverizer, the stalk fragment of acquisition is less than 2cm, as handled thing.Urea is added in the ratio of 10kg urea/ton stalk in handled thing, animal excrement are added in the ratio of 50kg animal excrement/ton stalk, complex micro organism fungicide prepared by step () is added in the ratio of 8kg complex micro organism fungicide/ton stalk, ferment 30 days, obtained cornstalk biological organic fertilizer material.
Claims (2)
1. a preparation method for bio-organic fertilizer, comprises the steps:
(1) preparation of complex micro organism fungicide:
The active ingredient of described complex micro organism fungicide is subtilis (Bacillus subtilis), aspergillus oryzae (Aspergillus niger), Trichodermareesei (Trichoderma viride), whiterot fungi (Phanerochaetechrysosporium), Geobacillus stearothermophilus (Geobacillus stearothermophilus), and described complex micro organism fungicide prepares with the following method:
1. actication of culture: each bacterial classification described above is inoculated on slant medium respectively; Subtilis 28 ~ 32 DEG C, pH is 7.2 ~ 7.4, and incubation time is 1 ~ 3 day; Aspergillus oryzae culture temperature 25 DEG C, incubation time is 4 ~ 7 days; Trichodermareesei culture temperature 25 ~ 28 DEG C, natural PH, incubation time is 4 ~ 7 days; Whiterot fungi culture temperature 25 ~ 28 DEG C, incubation time is 4 ~ 7 days; Geobacillus stearothermophilus culture temperature 60 DEG C, PH6.8, incubation time is 1 ~ 3 day;
2. first class inoculum is cultivated: be inoculated in respectively by each bacterial classification that step (1) activates in liquid nutrient medium corresponding to each bacterial classification, shaking table shaking culture, until bacterial classification living bacteria count is respectively 10 in each bacteria culture fluid
6-10
8individual/ml;
3. second order fermentation: step (2) each bacteria culture fluid is inoculated in strain fermentation substratum respectively by inoculum size 1-10%, shaking table shaking culture, until bacterial classification living bacteria count is respectively 10 in each strain fermentation nutrient solution
6-10
8individual/mL, collect fermentation culture, respectively each strain fermentating liquid;
4. each strain fermentating liquid that step (3) is obtained is mixed, obtain mixed bacteria liquid; In mixed bacteria liquid, fermentation of bacillus subtilis nutrient solution accounts for 15 ~ 20wt%, aspergillus oryzae fermentation culture accounts for 15 ~ 20wt%, and Trichodermareesei fermentation culture accounts for 30 ~ 40wt%, whiterot fungi fermentation culture accounts for 10 ~ 15wt%, Geobacillus stearothermophilus fermentation culture accounts for 10 ~ 20wt%;
5. three grade fermemtation: be inoculated in composite fungus agent substratum by inoculum size 1 ~ 5% by mixed bacteria liquid, cultivates 4 ~ 7 days, makes bacterium number in fermented liquid reach 10 at 30 DEG C
6~ 10
8individual/mL, obtains complex micro organism fungicide;
(2) preparation of bio-organic fertilizer
Stalk pulverizer is ground into the segment being less than 2cm, adds the complex micro organism fungicide of step (1) in the ratio of 1 ~ 8kg complex micro organism fungicide/ton stalk, 25 ~ 30 DEG C of fermentations 15 ~ 40 days.
2. the preparation method of bio-organic fertilizer according to claim 1, it is characterized in that, in step (2): stalk pulverizer is ground into the segment being less than 2cm, urea is added in the ratio of 1-10kg urea/ton stalk, animal excrement are added in the ratio of 10-100kg animal excrement/ton stalk, the complex micro organism fungicide of step (1) is added, at 25-30 DEG C of fermentation 15-40 days in the ratio of 1-8kg complex micro organism fungicide/ton stalk.
Priority Applications (1)
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|---|---|---|---|
| CN201310665870.6A CN104692844A (en) | 2013-12-06 | 2013-12-06 | Preparation method of bioorganic fertilizer |
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|---|---|---|---|
| CN201310665870.6A CN104692844A (en) | 2013-12-06 | 2013-12-06 | Preparation method of bioorganic fertilizer |
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| CN104692844A true CN104692844A (en) | 2015-06-10 |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105819913A (en) * | 2016-03-14 | 2016-08-03 | 北京天工环境治理有限责任公司 | Composite bacterial fertilizer, preparation method and application thereof |
| CN105859383A (en) * | 2016-03-31 | 2016-08-17 | 四川沃达丰生物科技有限公司 | Microbial organic fertilizer and preparation method therefor |
| CN106008101A (en) * | 2016-05-25 | 2016-10-12 | 山西凯盛肥业集团有限公司 | Composite microbial organic fertilizer for saline-alkali soil improvement and preparation method thereof |
| CN105994382A (en) * | 2016-06-08 | 2016-10-12 | 纪新强 | Preparation method for microbial agent used for agriculture |
| CN106187369A (en) * | 2016-07-06 | 2016-12-07 | 贵州省烟草公司黔西南州公司 | A kind of tobacco special organic inorganic compound fertilizer and preparation method thereof |
| CN107056544A (en) * | 2017-04-27 | 2017-08-18 | 河南农业大学 | One kind promotes root growth high carbon-based soil remediation fertilizer and method |
| CN107540427A (en) * | 2016-06-28 | 2018-01-05 | 益阳园丰生物科技有限公司 | A kind of organic fertilizer |
| CN107793201A (en) * | 2017-11-16 | 2018-03-13 | 陕西科技大学 | It is a kind of using apple tree beta pruning and its method for superseded production of fruit trees biological organic fertilizer |
| CN108504607A (en) * | 2018-04-24 | 2018-09-07 | 辽宁弘昊环境工程有限公司 | Microbial fermentation inoculum and its application and the method that fermentation animal wastes are organic fertilizer |
| CN111647527A (en) * | 2020-05-21 | 2020-09-11 | 浙江工业大学 | Method for improving saline-alkali soil and preserving fertilizer by utilizing bacillus subtilis |
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2013
- 2013-12-06 CN CN201310665870.6A patent/CN104692844A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105819913A (en) * | 2016-03-14 | 2016-08-03 | 北京天工环境治理有限责任公司 | Composite bacterial fertilizer, preparation method and application thereof |
| CN105859383A (en) * | 2016-03-31 | 2016-08-17 | 四川沃达丰生物科技有限公司 | Microbial organic fertilizer and preparation method therefor |
| CN106008101A (en) * | 2016-05-25 | 2016-10-12 | 山西凯盛肥业集团有限公司 | Composite microbial organic fertilizer for saline-alkali soil improvement and preparation method thereof |
| CN105994382A (en) * | 2016-06-08 | 2016-10-12 | 纪新强 | Preparation method for microbial agent used for agriculture |
| CN105994382B (en) * | 2016-06-08 | 2018-08-17 | 山东多芬农业有限公司 | A kind of preparation method of agricultural micro organism microbial inoculum |
| CN107540427A (en) * | 2016-06-28 | 2018-01-05 | 益阳园丰生物科技有限公司 | A kind of organic fertilizer |
| CN106187369A (en) * | 2016-07-06 | 2016-12-07 | 贵州省烟草公司黔西南州公司 | A kind of tobacco special organic inorganic compound fertilizer and preparation method thereof |
| CN107056544A (en) * | 2017-04-27 | 2017-08-18 | 河南农业大学 | One kind promotes root growth high carbon-based soil remediation fertilizer and method |
| CN107793201A (en) * | 2017-11-16 | 2018-03-13 | 陕西科技大学 | It is a kind of using apple tree beta pruning and its method for superseded production of fruit trees biological organic fertilizer |
| CN108504607A (en) * | 2018-04-24 | 2018-09-07 | 辽宁弘昊环境工程有限公司 | Microbial fermentation inoculum and its application and the method that fermentation animal wastes are organic fertilizer |
| CN111647527A (en) * | 2020-05-21 | 2020-09-11 | 浙江工业大学 | Method for improving saline-alkali soil and preserving fertilizer by utilizing bacillus subtilis |
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Application publication date: 20150610 |