CN104694410A - Preparation method of straw decomposition agent - Google Patents
Preparation method of straw decomposition agent Download PDFInfo
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- CN104694410A CN104694410A CN201310665845.8A CN201310665845A CN104694410A CN 104694410 A CN104694410 A CN 104694410A CN 201310665845 A CN201310665845 A CN 201310665845A CN 104694410 A CN104694410 A CN 104694410A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Soil Conditioners And Soil-Stabilizing Materials (AREA)
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a preparation method of a straw decomposition agent. The straw decomposition agent is prepared by using bacillus subtilis, aspergillus oryzae, lactobacillus plantarum, trichoderma reesei and saccharomyces cerevisiae as preparation strains, carrying out activation and primary and secondary culture on the strains respectively to obtain various strain solutions with bacterial count of 106-108/ml and mixing the strain solutions according to a certain proportion. The straw decomposition agent has the beneficial effects that the straw decomposition agent can be used for quickly degrading straws to prepare fertilizers and ensures that the straw decomposition time is at least 15 days ahead of the normal composting fermentation time; organic straw fertilizers prepared by the preparation method are used as base fertilizers, so that the contents of available nitrogen, rapidly available phosphorus, potassium and organic matters in soil are higher than those of available nitrogen, rapidly available phosphorus, potassium and organic matters in the soil in which straws are directly used and fertilizers are not applied; the urease activity and cellulase activity of the soil are obviously improved compared with those of the soil in which straws are directly used and fertilizers are not applied.
Description
Technical field
The present invention relates to a kind of decomposing agent, especially relate to a kind of preparation method of decomposing agent of degrading straw.
Background technology
Agricultural crop straw is a kind of huge resource, has that quantity is large, kind is many, widespread feature.According to statistics, China's stalk annual production is about about 700,000,000 tons, but straw utilization efficiency is extremely low, and most of stalk is wantonly burned, puts aside as useless, causes serious environmental pollution and the wasting of resources.Current stalk mostly is direct returning to farmland at Land use systems agriculturally, but because cold ground soil straw-returning decomposition rate is slow and cause the soil moisture to reduce, affects plant growth, so cause the ratio of straw-returning always lower.The optimal path that straw-returning addresses these problems beyond doubt is realized by compost.Volume and weight that germ in refuse and grass seeds, reduction store up can be killed by composting, be conducive to storage and use.Not only can solve the problem of environmental pollution of large-scale cultivation factory and burning straw generation, and to developing fertilizer, keeping and increase soil fertility, promote that agricultural sustainable development is significant.
Straw compost is as the effective way of a kind of solid waste minimizing, resource utilization, and its digest process carries out in the dynamic process of biological community structure succession.But Natural compost required time is long, and straw utilization rate is lower, is therefore badly in need of a kind of efficient complex microbial inoculum of development and improves the speed of becoming thoroughly decomposed of stalk and rate of becoming thoroughly decomposed.
Summary of the invention
The invention provides the straw decomposing inoculant that one kind of multiple bacterial classification microbial inoculums mix, this straw decomposing inoculant makes full use of the physiological activity of bacterial classification, produce the biological enzymes such as Mierocrystalline cellulose, hemicellulose and lignoenzyme, accelerate the maturity of stalk, thus prepare bio-organic fertilizer.
Technical scheme of the present invention is as follows:
A kind of preparation method of straw decomposing inoculant, this straw decomposing inoculant is subtilis (Bacillussubtilis) microbial inoculum, aspergillus oryzae (Aspergillus niger) microbial inoculum, plant lactobacillus (Lactobacillusplantarum) microbial inoculum, Trichodermareesei (Trichoderma viride) microbial inoculum, S. cervisiae (Saccharomycescerevisiae) microbial inoculum mix, and concrete grammar is as follows:
(1) described each bacterial classification is inoculated on the general slant medium of each bacterial classification respectively cultivates; Wherein subtilis, aspergillus oryzae, plant lactobacillus, Trichodermareesei, S. cervisiae are at 25-30 DEG C, cultivate 1-5 days;
(2) single bacterium colony of each bacterial classification of cultivating of selecting step (1), is inoculated in the general liquid nutrient medium of each bacterial classification, shaking table shaking culture, until bacterial classification living bacteria count is respectively 10 in each bacteria culture fluid respectively
6-10
8individual/ml;
(3) step (2) each bacteria culture fluid is inoculated in strain fermentation substratum respectively by inoculum size 1-5%, shaking table shaking culture, until bacterial classification living bacteria count is respectively 10 in each strain fermentation nutrient solution
6-10
8individual/mL, collect fermentation culture, respectively each bacterial classification microbial inoculum;
(4) by weight each bacterial classification microbial inoculum that step (3) obtains is mixed, obtain straw decomposing inoculant; Bacillus subtilis microbial agent 15-20 part, aspergillus oryzae microbial inoculum 15-20 part, plant lactobacillus microbial inoculum 20-30 part, Trichodermareesei microbial inoculum 10-30 part, yeast saccharomyces cerevisiae bacteria agent 15-25 part is comprised by weight in described straw decomposing inoculant.
In technical scheme of the present invention, the described strain fermentation substratum of step (3) is: the straw 13.4g/L of explosion, peptone 11g/L, ammonium sulfate 13g/L, glucose 6g/L, potassium primary phosphate 10.5g/L.
In technical scheme of the present invention, when described in step (3), each bacterial classification ferments in strain fermentation nutrient solution, leavening temperature is 25-32 DEG C, and fermentation time is 1-4 days.
The invention has the beneficial effects as follows: straw decomposing inoculant of the present invention, bacterial classification assembly is a kind of new flora assembly, makes full use of the physiological activity of bacterial classification, produces the biological enzymes such as Mierocrystalline cellulose, hemicellulose and lignoenzyme, accelerate the maturity of stalk, thus prepare bio-organic fertilizer.Adopt straw decomposing inoculant energy fast degradation straw of the present invention for fertilizer, at least shift to an earlier date 15 days than the rotten fermentation time of normal heap.Straw organic fertilizer prepared by the present invention is used as base manure, soil alkaline hydrolysis nitrogen, rapid available phosphorus, potassium and organic content are all high than directly using the soil content of stalk and non-applying fertilizer, and soil urease liveness and cellulase activity are also all than directly using the soil of stalk and non-applying fertilizer to significantly improve.
Embodiment
Following non-limiting example can make the present invention of those of ordinary skill in the art's comprehend, but does not limit the present invention in any way.Test method described in embodiment, if no special instructions, is ordinary method; Described reagent, material and bacterial classification, if no special instructions, all can obtain from commercial channels, or can prepare in conventional manner.
Embodiment 1
A kind of straw decomposing inoculant, this straw decomposing inoculant is subtilis (Bacillus subtilis) microbial inoculum, aspergillus oryzae (Aspergillus niger) microbial inoculum, plant lactobacillus (Lactobacillus plantarum) microbial inoculum, Trichodermareesei (Trichoderma viride) microbial inoculum, S. cervisiae (Saccharomyces cerevisiae) microbial inoculum mix, and concrete grammar is as follows:
(1) described each bacterial classification is inoculated on the general slant medium of each bacterial classification respectively cultivates; Wherein subtilis 28 ~ 32 DEG C, pH is 7.2 ~ 7.4, and incubation time is 2 days; Aspergillus oryzae 25 DEG C, incubation time is 6 days; Plant lactobacillus 25 DEG C, incubation time is 2 days; Trichodermareesei culture temperature 25 ~ 28 DEG C, natural PH, incubation time is 3 days; S. cervisiae is at 28 ~ 32 DEG C, and pH is 7.2 ~ 7.4, and incubation time is 2 days;
(2) single bacterium colony of each bacterial classification of cultivating of selecting step (1), is inoculated in the general liquid nutrient medium of each bacterial classification, shaking table shaking culture, until bacterial classification living bacteria count is respectively 10 in each bacteria culture fluid respectively
7individual/ml;
(3) step (2) each bacteria culture fluid is inoculated in strain fermentation substratum respectively by inoculum size 2%, shaking table shaking culture, until bacterial classification living bacteria count is respectively 10 in each strain fermentation nutrient solution
7individual/mL, collect fermentation culture, respectively each bacterial classification microbial inoculum;
(4) by weight each bacterial classification microbial inoculum that step (3) obtains is mixed, obtain straw decomposing inoculant; Bacillus subtilis microbial agent 15 parts, aspergillus oryzae microbial inoculum 20 parts, plant lactobacillus microbial inoculum 20, Trichodermareesei microbial inoculum 10 parts, yeast saccharomyces cerevisiae bacteria agent 15 parts is comprised by weight in described straw decomposing inoculant.
Described strain fermentation substratum is: the straw 13.4g/L of explosion, peptone 11g/L, ammonium sulfate 13g/L, glucose 6g/L, potassium primary phosphate 10.5g/L.
The liquid nutrient medium of described each bacterial classification is:
The liquid culture based formulas that subtilis and plant lactobacillus adopt is: plain agar: beef infusion broth 1.0L, peptone 10g, NaCl5.0g, agar 20.0-20.5g, PH7.2-7.4, the preparation of beef infusion broth: lean beef is cleaned, chopping, takes 550g and is soaked in 1375ml water, soak a night, filter, after filtering packing, 0.6kgf/cm240min is for subsequent use;
The liquid culture based formulas that aspergillus oryzae adopts is: 5 ° of Be wort 1.0L, agar 15.0g, natural PH;
The liquid culture based formulas that Trichodermareesei and S. cervisiae adopt is: PDA substratum, remove the potato 200 grams of skin, be cut into small pieces, add water 1000 milliliters and boil 30 minutes elimination potato balls, filtrate is complemented to 1000 milliliters, add glucose 20 grams, 15 grams, agar, dissolve rear packing, 15 pounds of sterilizings 30 minutes, natural PH.
Embodiment 2
A kind of straw decomposing inoculant, this straw decomposing inoculant is bacillus subtilis microbial agent, aspergillus oryzae microbial inoculum, and plant lactobacillus microbial inoculum, Trichodermareesei microbial inoculum, yeast saccharomyces cerevisiae bacteria agent mix, and concrete grammar is as follows:
(1) described each bacterial classification is inoculated on the general slant medium of each bacterial classification respectively cultivates; Wherein subtilis 28 ~ 32 DEG C, pH is 7.2 ~ 7.4, and incubation time is 2 days; Aspergillus oryzae 25 DEG C, incubation time is 6 days; Plant lactobacillus 25 DEG C, incubation time is 2 days; Trichodermareesei culture temperature 25 ~ 28 DEG C, natural PH, incubation time is 3 days; S. cervisiae is at 28 ~ 32 DEG C, and pH is 7.2 ~ 7.4, and incubation time is 2 days;
(2) single bacterium colony of each bacterial classification of cultivating of selecting step (1), is inoculated in the general liquid nutrient medium of each bacterial classification, shaking table shaking culture, until bacterial classification living bacteria count is respectively 10 in each bacteria culture fluid respectively
8individual/ml;
(3) step (2) each bacteria culture fluid is inoculated in strain fermentation substratum respectively by inoculum size 2%, shaking table shaking culture, until bacterial classification living bacteria count is respectively 10 in each strain fermentation nutrient solution
8individual/mL, collect fermentation culture, respectively each bacterial classification microbial inoculum;
(4) by weight each bacterial classification microbial inoculum that step (3) obtains is mixed, obtain straw decomposing inoculant; Bacillus subtilis microbial agent 20 parts, aspergillus oryzae microbial inoculum 15 parts, plant lactobacillus microbial inoculum 30, Trichodermareesei microbial inoculum 25 parts, yeast saccharomyces cerevisiae bacteria agent 25 parts is comprised by weight in described straw decomposing inoculant.
The formula of the liquid nutrient medium of described strain fermentation substratum, each bacterial classification is with embodiment 1.
Claims (3)
1. a preparation method for straw decomposing inoculant, is characterized in that, this straw decomposing inoculant is bacillus subtilis microbial agent, aspergillus oryzae microbial inoculum, plant lactobacillus microbial inoculum, Trichodermareesei microbial inoculum, yeast saccharomyces cerevisiae bacteria agent mix, and preparation method comprises the steps:
(1) described each bacterial classification is inoculated on the general slant medium of each bacterial classification respectively cultivates; Wherein subtilis, aspergillus oryzae, plant lactobacillus, Trichodermareesei, S. cervisiae are at 25-30 DEG C, cultivate 1-5 days;
(2) single bacterium colony of each bacterial classification of cultivating of selecting step (1), is inoculated in the general liquid nutrient medium of each bacterial classification, shaking table shaking culture, until bacterial classification living bacteria count is respectively 10 in each bacteria culture fluid respectively
6-10
8individual/ml;
(3) step (2) each bacteria culture fluid is inoculated in strain fermentation substratum by inoculum size 1-5% respectively, shaking table shaking culture, until bacterial classification living bacteria count is respectively 10 in each strain fermentation nutrient solution
6-10
8individual/mL, collect fermentation culture, respectively each bacterial classification microbial inoculum;
(4) by weight each bacterial classification microbial inoculum that step (3) obtains is mixed, obtain straw decomposing inoculant; Bacillus subtilis microbial agent 15-20 part, aspergillus oryzae microbial inoculum 15-20 part, plant lactobacillus microbial inoculum 20-30 part, Trichodermareesei microbial inoculum 10-30 part, yeast saccharomyces cerevisiae bacteria agent 15-25 part is comprised by weight in described straw decomposing inoculant.
2. preparation method according to claim 1, is characterized in that, the described strain fermentation substratum of step (3) is: the straw 13.4g/L of explosion, peptone 11g/L, ammonium sulfate 13g/L, glucose 6g/L, potassium primary phosphate 10.5g/L.
3. preparation method according to claim 1, is characterized in that, when described in step (3), each bacterial classification ferments in strain fermentation nutrient solution, leavening temperature is 25-32 DEG C, and fermentation time is 1-4 days.
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| CN201310665845.8A CN104694410A (en) | 2013-12-06 | 2013-12-06 | Preparation method of straw decomposition agent |
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| CN201310665845.8A CN104694410A (en) | 2013-12-06 | 2013-12-06 | Preparation method of straw decomposition agent |
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105061041A (en) * | 2015-08-05 | 2015-11-18 | 马鞍山科邦生态肥有限公司 | Novel bio-organic-inorganic compound fertilizer and preparation method thereof |
| CN105777234A (en) * | 2016-05-31 | 2016-07-20 | 沈阳市现代三川生物技术研究所 | Method for preparing biological organic fertilizer through common fermentation of nitrogen-fixing bacteria, phosphate solubilizing bacteria and potassium bacteria |
| CN106107062A (en) * | 2016-07-25 | 2016-11-16 | 滨州中裕食品有限公司 | A kind of bioanalysis straw ensiling new technology |
| CN106242829A (en) * | 2016-08-26 | 2016-12-21 | 尹求实 | A kind of overcome the soil remediation fertilizer of Radix Salviae Miltiorrhizae continuous cropping obstacle, preparation method and its usage |
| CN106278654A (en) * | 2016-08-26 | 2017-01-04 | 尹求实 | A kind of overcome the soil remediation fertilizer of Rhizoma Zingiberis Recens continuous cropping obstacle, preparation method and its usage |
| CN106306426A (en) * | 2016-08-20 | 2017-01-11 | 裕龙农牧科技股份有限公司 | Novel process for corn silage |
| CN106365729A (en) * | 2016-08-26 | 2017-02-01 | 尹求实 | Soil remediation organic fertilizer for overcoming panax notoginseng continuous cropping obstruction, preparation method and application of soil remediation organic fertilizer |
| CN106380269A (en) * | 2016-08-26 | 2017-02-08 | 尹求实 | Soil remediation organic fertilizer for overcoming watermelon replantation obstacle, preparation method thereof and application thereof |
| CN106811438A (en) * | 2017-04-10 | 2017-06-09 | 河南天冠纤维乙醇有限公司 | A kind of straw degradative acidifying microbial inoculum and preparation method thereof |
| CN107162837A (en) * | 2016-03-08 | 2017-09-15 | 勐海茶业有限责任公司 | A kind of tea grounds organic fertilizer and preparation method thereof |
| CN109423462A (en) * | 2017-09-04 | 2019-03-05 | 上海康地源生物科技有限公司 | One kind being used for straw and the decomposed complex microorganism preparations manufacturing method of animal wastes |
| CN109679857A (en) * | 2018-12-25 | 2019-04-26 | 广东高龙环保科技有限公司 | A kind of compost maturity microbial inoculum |
| CN109943499A (en) * | 2019-02-25 | 2019-06-28 | 车团结 | A kind of straw decomposing inoculant and its preparation and application |
| CN112457070A (en) * | 2020-11-18 | 2021-03-09 | 上海玖沁环保科技有限公司 | Environment-friendly biological agent for treating straws |
-
2013
- 2013-12-06 CN CN201310665845.8A patent/CN104694410A/en active Pending
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105061041A (en) * | 2015-08-05 | 2015-11-18 | 马鞍山科邦生态肥有限公司 | Novel bio-organic-inorganic compound fertilizer and preparation method thereof |
| CN107162837A (en) * | 2016-03-08 | 2017-09-15 | 勐海茶业有限责任公司 | A kind of tea grounds organic fertilizer and preparation method thereof |
| CN105777234A (en) * | 2016-05-31 | 2016-07-20 | 沈阳市现代三川生物技术研究所 | Method for preparing biological organic fertilizer through common fermentation of nitrogen-fixing bacteria, phosphate solubilizing bacteria and potassium bacteria |
| CN106107062A (en) * | 2016-07-25 | 2016-11-16 | 滨州中裕食品有限公司 | A kind of bioanalysis straw ensiling new technology |
| CN106306426A (en) * | 2016-08-20 | 2017-01-11 | 裕龙农牧科技股份有限公司 | Novel process for corn silage |
| CN106242829A (en) * | 2016-08-26 | 2016-12-21 | 尹求实 | A kind of overcome the soil remediation fertilizer of Radix Salviae Miltiorrhizae continuous cropping obstacle, preparation method and its usage |
| CN106365729A (en) * | 2016-08-26 | 2017-02-01 | 尹求实 | Soil remediation organic fertilizer for overcoming panax notoginseng continuous cropping obstruction, preparation method and application of soil remediation organic fertilizer |
| CN106380269A (en) * | 2016-08-26 | 2017-02-08 | 尹求实 | Soil remediation organic fertilizer for overcoming watermelon replantation obstacle, preparation method thereof and application thereof |
| CN106278654A (en) * | 2016-08-26 | 2017-01-04 | 尹求实 | A kind of overcome the soil remediation fertilizer of Rhizoma Zingiberis Recens continuous cropping obstacle, preparation method and its usage |
| CN106811438A (en) * | 2017-04-10 | 2017-06-09 | 河南天冠纤维乙醇有限公司 | A kind of straw degradative acidifying microbial inoculum and preparation method thereof |
| CN106811438B (en) * | 2017-04-10 | 2020-12-04 | 河南天冠纤维乙醇有限公司 | Straw degradation acidification microbial inoculum and preparation method thereof |
| CN109423462A (en) * | 2017-09-04 | 2019-03-05 | 上海康地源生物科技有限公司 | One kind being used for straw and the decomposed complex microorganism preparations manufacturing method of animal wastes |
| CN109679857A (en) * | 2018-12-25 | 2019-04-26 | 广东高龙环保科技有限公司 | A kind of compost maturity microbial inoculum |
| CN109943499A (en) * | 2019-02-25 | 2019-06-28 | 车团结 | A kind of straw decomposing inoculant and its preparation and application |
| CN112457070A (en) * | 2020-11-18 | 2021-03-09 | 上海玖沁环保科技有限公司 | Environment-friendly biological agent for treating straws |
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Application publication date: 20150610 |