CN109423462A - One kind being used for straw and the decomposed complex microorganism preparations manufacturing method of animal wastes - Google Patents
One kind being used for straw and the decomposed complex microorganism preparations manufacturing method of animal wastes Download PDFInfo
- Publication number
- CN109423462A CN109423462A CN201710783692.5A CN201710783692A CN109423462A CN 109423462 A CN109423462 A CN 109423462A CN 201710783692 A CN201710783692 A CN 201710783692A CN 109423462 A CN109423462 A CN 109423462A
- Authority
- CN
- China
- Prior art keywords
- bacillus
- culture
- culture medium
- bacterium
- complex microorganism
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Mycology (AREA)
- Botany (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses one kind to be used for straw and the decomposed complex microorganism preparations manufacturing method of animal wastes, technical solution is to bacillus megaterium, bacillus subtilis, bacillus licheniformis, bacillus stearothermophilus, saccharomyces cerevisiae, trichodermaharzianum bacterium, black-koji mould, aspergillus oryzae is individually cultivated, and expanding culture is also individually to be cultivated, original seed is provided by Shanghai City Industry Wei Biological Research Institute, it is obtained by screening domestication, it uses purebred culture to obtain 200,000,000/ml of technical indicator that bacterium solution living bacteria count is much higher than agricultural microbial inoculum product, and miscellaneous bacteria is free of in preparation.
Description
Technical field
The present invention relates to straw and the decomposed complex microorganism preparations manufacturing method of animal wastes is used for, belong to organic fertilizer
Expect fermentation technical field, the in particular to component and compatibility of complex microorganism preparations.
Background technique
China is a large agricultural country, and a large amount of stalk is generated in grain-production, according to statistics, China's stalk total amount in 2016
Close to 800,000,000 tons, is directly burned or the quantity that arbitrarily abandons is at 1.3 ~ 1.6 hundred million tons, account for total amount 17 ~ 20%, this is not only agriculture money
Source significant wastage, also can cause environmental pollution.Therefore straw and animal wastes are mixed and passes through microbial fermentation technology
Good organic fertilizer is made, is stalk and the optimal Land use systems of excrement, provides a large amount of good organic fertilizer for agricultural production,
This is to increase soil fertility, it is ensured that agricultural foison has great meaning.
Summary of the invention
The purpose of the present invention is provide a kind of for straw and animal to change the status that the above stalk largely wastes
The technical solution of the decomposed complex microorganism preparations manufacturing method of excrement.Technical formula of the invention: by bacillus megaterium, withered grass
Bacillus, bacillus licheniformis, bacillus stearothermophilus, S. cervisiae, trichodermaharzianum bacterium, black-koji mould, aspergillus oryzae
Bacterium composition, original seed are provided by Shanghai City Industry Wei Biological Research Institute, are tamed and are obtained by screening.Compound formulation of the invention is divided into
Bacillus category, der Pilz, S. cervisiae, four major class bacterium of bacillus stearothermophilus, four major class bacterium solutions are in complex microorganism
Ratio in preparation are as follows: bacillus category 30-50%, bacillus stearothermophilus are 10 ~ 20%, and der Pilz is 20 ~ 30%, ferment of making wine
Female bacterium is 5 ~ 10%.It cultivates mycorhiza and provides different culture medium prescriptions, growth temperature, optimal pH, ventilation according to every kind of bacterium own characteristic
Amount and incubation time, to ensure that each bacterial strain is grown at optimum conditions；In expanding culture, if the strain of different attribute is put
Culture is very wrong together, and usually only yeast and mold can be compared with good utilisation saccharine material.The solution of the present invention is adopted
(including expanding culture) is individually cultivated with every kind of bacterium, is finally configured to compound formulation in proportion, and it is very high to guarantee that every kind of strain has
Activity.It is characterized by: the bacillus megaterium, bacillus subtilis and bacillus licheniformis use culture medium,
It is cultivated 24 hours under the conditions of 35 DEG C, PH7.2,180rpm, culture medium prescription is peptone 10.0g, beef extract 3.0g, sodium chloride
5.0g, distilled water 1000ml, PH sterilize 30 minutes between 7.2-7.5 in 121 DEG C；
The bacillus stearothermophilus is cultivated 24 hours under conditions of 55 DEG C, PH7.0,180rpm using culture medium, trains
Support based formulas are as follows: beef extract 5.0g, soy peptone 9.0g, sodium chloride 2.0g, K2HPO4 1.0g、KH2PO40.75g, distillation
Between 6.8 ~ 7.2,121 DEG C sterilize 30 minutes for water 1000ml, pH value；
Trichodermaharzianum, aspergillus niger, aspergillus oryzae and the S. cervisiae using culture medium 28 DEG C, natural pH value,
Cultivate 48 hours under conditions of 180rpm, culture medium prescription: 30 minutes potato 200g, glucose 20.0g are boiled in stripping and slicing, steam
Distilled water 1000ml boils 30 minutes, natural pH value, and 121 DEG C sterilize 30 minutes.
It prepares the specific method of seed liquor: respectively moving to bacillus subtilis, bacillus megaterium, bacillus licheniformis
In 500ml triangular flask, bottle is provided with 200ml bacteria culture media, at 35 DEG C, cultivates 24 hours under the conditions of 180rpm；Respectively by health
Peaceful trichoderma, aspergillus niger, aspergillus oryzae and fermented glutinous rice yeast bacterium move in 500ml triangular flask, and bottle is provided with 200ml bacteria culture media,
It 28 DEG C, is cultivated 48 hours under the conditions of 180rpm；Bacillus stearothermophilus is moved in 500ml triangular flask, bottle is provided with 200ml
Bacteria culture media is cultivated 24 hours under the conditions of 180rpm at 55 DEG C.
The expansion culture of seed liquor:
Each seed liquor is extended in 5000ml triangular flask by 5% inoculum concentration, and bottle is provided with culture medium 2000ml, is trained according to seed liquor
Support CMC model；Secondary seed solution is finally extended to 500 liters of fermentors, and 200 liters of the built-in culture medium of tank, fermentation condition is also according to kind
Sub- liquid condition of culture is cultivated.Then cultured various bacterium solutions are examined according to according to effective viable bacteria standard detecting method
It surveys, bacillus megaterium, bacillus subtilis and the effective bacterium numberical range of bacillus licheniformis liquid are 400 ~ 60,000,000,000 cfu/
Ml, the effective bacterium number range of stearothermophilus gemma bacteria liquid are 100 ~ 120cfu, hundred million/ml, trichodermaharzianum, aspergillus niger, aspergillus oryzae liquid
Effective bacterium numberical range is 60 ~ 8,000,000,000 cfu/ml, and the effective bacterium number range of saccharomyces cerevisiae bacteria liquid is 30 ~ 6,000,000,000 cfu/ml.
The strain compatibility of complex microorganism preparations: under the conditions of laboratory scale, above-mentioned 8 kinds of bacterium solutions are passed through into organic matter corruption
Ripe orthogonal test, to ensure various strains optimal proportion in compound formulation.
Obvious advantage and effect after implementing the technology are: obtaining bacterium solution living bacteria count much higher than agriculture using purebred culture
With 200,000,000 cfu/ml of technical indicator of microbial inoculum product, and miscellaneous bacteria is free of in preparation.
Specific embodiment
It is stalk and the decomposed test of animal wastes below, is not intended to limit the scope of the invention:
First to stalk pretreatment: water intaking rice straw is cut into broken or crushes, and watering is drenched plus a small amount of milk of lime is mixed thoroughly a couple of days, controls
Material pH value is between 9.0 ~ 9.5.
The decomposed test of stalk: add 20% or so pig manure or chicken manure in stalk after treatment, control material carbon, nitrogen ratio
In 25 ~ 30:1, total moisture content between 6.5 ~ 7.5, piles certain altitude after adding 0.2 ~ 0.4% composite bacteria agent to mix in 55 ~ 60%, PH
It ferments, monitors the temperature and water content of material daily, just need turning to cool down when the temperature of material rises to 65 DEG C.This
Outside, turning can also homogeneous material and timely oxygenating accelerate digest process, be organic fertilizer through fermentation in 10 days.
The decomposed quality standard of product:
1, product is in dark brown, and the loose odorless of granule places a couple of days rear surface president white or grey mould；
2, the germination coefficient of product is up to 80 ~ 90%；
3, product reaches innoxious sanitary standard: induced worm egg death rate is up to 95 ~ 100%, and excrement colibacillus group value is up to 1 × 10-2~1×
10-1CFU/g。
Claims (3)
1. one kind is used for straw and the decomposed complex microorganism preparations manufacturing method of animal wastes, it is characterised in that: described
Complex microorganism preparations are made of bacillus category, der Pilz, saccharomyces cerevisiae, four major class of bacillus stearothermophilus, are specifically matched
Side are as follows: bacillus megaterium, bacillus subtilis, bacillus licheniformis, bacillus stearothermophilus, S. cervisiae, healthy and free from worry
Trichoderma, black-koji mould, aspergillus oryzae, four major class bacterium solution ratios in complex microorganism preparations are as follows: bacillus category 30-50%,
Bacillus stearothermophilus is 10 ~ 20%, and der Pilz is 20 ~ 30%, and saccharomyces cerevisiae is 5 ~ 10%.
2. complex microorganism preparations according to claim 1, it is characterised in that: the bacillus megaterium, withered grass bud
Spore bacillus and bacillus licheniformis use culture medium culture, and culture medium prescription is peptone 1.0g, beef extract 3.0g, sodium chloride
5.0g, distilled water 1000ml, PH sterilize 30 minutes between 7.2-7.5 in 121 DEG C；The bacillus stearothermophilus is adopted
With culture medium culture, culture medium prescription are as follows: beef extract 5.0g, soy peptone 9.0g, sodium chloride 2.0g, K2HPO4 1.0g、
KH2PO4Between 6.8 ~ 7.2,121 DEG C sterilize 30 minutes for 0.75g, distilled water 1000ml, pH value；The trichodermaharzianum bacterium,
Black-koji mould, aspergillus oryzae and S. cervisiae use culture medium culture, culture medium prescription are as follows: 30 minutes Ma Ling are boiled in stripping and slicing
Potato 200g, glucose 20.0g, distilled water 1000ml, natural pH value, 121 DEG C sterilize 30 minutes.
3. complex microorganism preparations manufacturing method according to claim 1, it is characterised in that: the following steps are included:
Seed liquor culture: respectively moving to bacillus subtilis, bacillus megaterium, bacillus licheniformis in 500ml triangular flask,
Bottle is provided with 200ml culture medium, at 35 DEG C, cultivates 24 hours under the conditions of 180rpm；Respectively by trichodermaharzianum, aspergillus niger, meter Qu
Mould and fermented glutinous rice yeast moves in 500ml triangular flask, and bottle is provided with 200ml bacteria culture media, at 28 DEG C, cultivates under the conditions of 180rpm
48 hours；Bacillus stearothermophilus is moved in 500ml triangular flask, bottle is provided with 200ml culture medium, at 55 DEG C, 180rpm
Under the conditions of cultivate 24 hours；
The expansion culture of seed liquor: each seed liquor is extended in 5000ml triangular flask by 5% inoculum concentration, and bottle is provided with culture medium
2000ml, according to seed liquor condition of culture culture；Secondary seed solution is finally extended to 500 liters of fermentors, the built-in culture medium 200 of tank
It rises, fermentation condition is also cultivated according to seed liquor condition of culture；According to effective viable bacteria standard detecting method to cultured each
Kind bacterium solution is detected, and bacillus megaterium, bacillus subtilis and the effective bacterium numberical range of bacillus licheniformis liquid are 400
~ 600 hundred million cuf/ml, the effective bacterium number range of stearothermophilus gemma bacteria liquid be 100 ~ 12,000,000,000 cuf/ml, trichodermaharzianum, aspergillus niger,
The effective bacterium numberical range of aspergillus oryzae liquid is 60 ~ 8,000,000,000 cuf/ml, and the effective bacterium number range of saccharomyces cerevisiae bacteria liquid is 30 ~ 6,000,000,000
cuf/ml。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710783692.5A CN109423462A (en) | 2017-09-04 | 2017-09-04 | One kind being used for straw and the decomposed complex microorganism preparations manufacturing method of animal wastes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710783692.5A CN109423462A (en) | 2017-09-04 | 2017-09-04 | One kind being used for straw and the decomposed complex microorganism preparations manufacturing method of animal wastes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109423462A true CN109423462A (en) | 2019-03-05 |
Family
ID=65505150
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710783692.5A Withdrawn CN109423462A (en) | 2017-09-04 | 2017-09-04 | One kind being used for straw and the decomposed complex microorganism preparations manufacturing method of animal wastes |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109423462A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110373348A (en) * | 2019-06-27 | 2019-10-25 | 苏州和青环境科技有限公司 | A kind of biological high-efficiency composite bacteria agent for decomposing and handling for waste |
| CN111849818A (en) * | 2020-07-27 | 2020-10-30 | 浙江嘉冠环保股份有限公司 | Biological aerobic fermentation bacteria |
| CN114752521A (en) * | 2022-03-22 | 2022-07-15 | 浙江宜葆生物科技有限公司 | Agricultural straw waste aerobic fermentation bacterium combined fungus bag |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101294141A (en) * | 2007-04-28 | 2008-10-29 | 上海四季生物科技有限公司 | A set of living body microorganism preparations for preparing composite microorganism fertilizer and preparation method thereof |
| CN102577985A (en) * | 2012-02-27 | 2012-07-18 | 武威市金绿源农业科技发展有限公司 | Pig-raising fermentation bacterial agent for biological and environment-friendly fermentation bed and preparation method thereof |
| CN103725631A (en) * | 2013-12-06 | 2014-04-16 | 大连三科生物工程有限公司 | Straw-decomposing inoculant |
| CN104694410A (en) * | 2013-12-06 | 2015-06-10 | 大连三科生物工程有限公司 | Preparation method of straw decomposition agent |
| CN105733975A (en) * | 2014-12-12 | 2016-07-06 | 河北省农林科学院遗传生理研究所 | Multifunctional high efficient organic material decomposition agent and preparation method thereof |
| WO2017138824A1 (en) * | 2016-02-10 | 2017-08-17 | Intermag Sp. Z O.O. | A microbiological preparation for the mineralization of cellulose -containing organic matter and the application of the microbiological preparation in growing plants and/or in animal nutrition |
-
2017
- 2017-09-04 CN CN201710783692.5A patent/CN109423462A/en not_active Withdrawn
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101294141A (en) * | 2007-04-28 | 2008-10-29 | 上海四季生物科技有限公司 | A set of living body microorganism preparations for preparing composite microorganism fertilizer and preparation method thereof |
| CN102577985A (en) * | 2012-02-27 | 2012-07-18 | 武威市金绿源农业科技发展有限公司 | Pig-raising fermentation bacterial agent for biological and environment-friendly fermentation bed and preparation method thereof |
| CN103725631A (en) * | 2013-12-06 | 2014-04-16 | 大连三科生物工程有限公司 | Straw-decomposing inoculant |
| CN104694410A (en) * | 2013-12-06 | 2015-06-10 | 大连三科生物工程有限公司 | Preparation method of straw decomposition agent |
| CN105733975A (en) * | 2014-12-12 | 2016-07-06 | 河北省农林科学院遗传生理研究所 | Multifunctional high efficient organic material decomposition agent and preparation method thereof |
| WO2017138824A1 (en) * | 2016-02-10 | 2017-08-17 | Intermag Sp. Z O.O. | A microbiological preparation for the mineralization of cellulose -containing organic matter and the application of the microbiological preparation in growing plants and/or in animal nutrition |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110373348A (en) * | 2019-06-27 | 2019-10-25 | 苏州和青环境科技有限公司 | A kind of biological high-efficiency composite bacteria agent for decomposing and handling for waste |
| CN111849818A (en) * | 2020-07-27 | 2020-10-30 | 浙江嘉冠环保股份有限公司 | Biological aerobic fermentation bacteria |
| CN114752521A (en) * | 2022-03-22 | 2022-07-15 | 浙江宜葆生物科技有限公司 | Agricultural straw waste aerobic fermentation bacterium combined fungus bag |
| CN114752521B (en) * | 2022-03-22 | 2024-04-12 | 浙江宜葆生物科技有限公司 | Combined fungus bag for aerobic fermentation fungus of agricultural straw waste |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101659934B (en) | Antagonistic bacteria preventing and removing continuous cropping banana Panama wilt disease and microbial organic fertilizer thereof | |
| CN1724481B (en) | Method of producing microbiological active organic fertilizer using waste mushroom dregs | |
| CN105462878B (en) | A kind of efficient decomposing agent of feces of livestock and poultry and its fermentation process | |
| CN104447095B (en) | A kind of biological activity organic medical fertilizer and its preparation method and application | |
| CN101948780B (en) | Antagonist bacterium for preventing and treating continuous cropping hot pepper epidemic disease and microbial organic fertilizer thereof | |
| CN107711891B (en) | A method of prevention matrimony vine root rot is extremely set | |
| CN103242083A (en) | Microbial organic fertilizer | |
| CN107365727A (en) | Organic fertilizer fermentation agent | |
| CN107646616A (en) | A kind of preparation method of floriculture substrate | |
| CN104726378B (en) | The method for improving salt stress turfgrass defence enzyme activity using Salt-tolerant microbial agent is strengthened | |
| CN108486010A (en) | A kind of During High-Temperature Composting bacterium composite bacteria agent and its application | |
| CN105255767B (en) | A kind of complex microorganism preparations and fowl fermenting bed padding | |
| CN105441360A (en) | Organic fertilizer fermentation inoculant | |
| CN107653200A (en) | A kind of microbial bacterial agent for promoting dead pig corpse aerobic compost and application | |
| CN107912267A (en) | A kind of preparation method of Chinese fir seedling medium | |
| CN105060944A (en) | Method for preparing egg white fertilizer by fish soluble | |
| CN103387428A (en) | Preparation method for organic material decomposition agent | |
| CN107177521A (en) | A kind of composite bacteria agent for the organic fertilizer that ferments and its preparation method and application | |
| CN109423462A (en) | One kind being used for straw and the decomposed complex microorganism preparations manufacturing method of animal wastes | |
| CN108821828A (en) | A kind of preparation method using the fermentation tailing liquor production material of liquid fertilizer containing polyglutamic acid | |
| CN108794091A (en) | A kind of organic fertilizer and preparation method thereof | |
| CN1653936B (en) | Method for preparing composite micro-ecological enzyme for feeding the ruminant by using disused mushroom dregs | |
| CN108676760A (en) | A kind of organic fertilizer decomposing agent and preparation method thereof | |
| CN109160862A (en) | A kind of amino acid bio-organic fertilizer and the preparation method and application thereof | |
| CN105886436A (en) | Composition or composite bacterium agent for degrading plant fiber waste |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20190305 |