CN107912267A - A kind of preparation method of Chinese fir seedling medium - Google Patents

A kind of preparation method of Chinese fir seedling medium Download PDF

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Publication number
CN107912267A
CN107912267A CN201711152384.9A CN201711152384A CN107912267A CN 107912267 A CN107912267 A CN 107912267A CN 201711152384 A CN201711152384 A CN 201711152384A CN 107912267 A CN107912267 A CN 107912267A
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parts
culture
particle
chinese fir
seedling medium
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杨伟
王远能
吴峰
韦荣洁
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Guangxi Rentaiiron Biological Technology Co Ltd
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Guangxi Rentaiiron Biological Technology Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F5/00Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
    • C05F5/002Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00Other inorganic fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80Soil conditioners
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

A kind of preparation method of Chinese fir seedling medium, step are:(1)Useless 45 60 parts of padding for pig sty is taken by weight;20 40 parts of Chinese mesona herb slag;15 25 parts of manioc waste;10 20 parts of straw particle;5 10 parts of ramulus mori particle;58 parts of corn ear slag particle;35 parts of bagasse particle;(2)Raw material is uniformly mixed to get premix;(3)Mixed Microbes are added into premix;(4)Premix and Mixed Microbes are put into tunnel fermentation kiln after mixing and are fermented;(5)By the premix after fermentation when pasteurization 8 12 is small after, obtain culture material;(6)It is placed on after culture material disinfection in edible fungus culturing canopy and is cooled to less than 25 DEG C, be implanted into edible fungus species, carries out edible fungus culturing;(7)After edible mushroom has been cultivated, Chinese fir branches and leaves particle is added, is turned uniformly, it is more than week to place one, then carry out turning, you can as masson pine seedling medium.The Chinese fir survival rate that this seedling medium is cultivated is high, and side fibrous root is flourishing.

Description

A kind of preparation method of Chinese fir seedling medium
Technical field
The invention belongs to Chinese fir planting technology field, is specifically a kind of preparation method of Chinese fir seedling medium.
Background technology
Chinese fir category Pinaceae, aiphyllium, is grown on the frigid zone of 2500 meters~4000 meters of height above sea level.It is high 30 meters reachable, 3 meters of the diameter of a cross-section of a tree trunk 1.3 meters above the ground, trunk end is straight, tree-like neat.The kind of China fir is more, is roughly divided into three classes:One kind is that spray young leaves is yellowish green Color, glossiness keteleeria fortunei, also known as Douglas fir, Chinese hemlock spruce;Another kind of is branches and leaves blue-green, lacklustre grey China fir, also known as chaff China fir, awns China fir, Steep China fir;Also one kind is that blade is thin pliable, the sagging line China fir of branch, also known as soft leaf China fir.It is referred to as " omnipotent wood ".Chinese fir Trunk texture is straight, and structure is careful, and material is soft, and corrosion resistant is mothproof, is widely used in building, bridge, shipbuilding, furniture etc..China Building materials to have a quarter be Chinese fir.The big tree of North America is originated in, is commonly called as " world grandfather ", is living organism maximum in the world.Through grinding Study carefully discovery, the robustness of Chinese fir nursery stock and the height of the surrival rate of afforestation have direct relation.How low cost, high-survival rate, It is that current Chinese fir plants major issue urgently to be resolved hurrily to cultivate Chinese fir stalwartness nursery stock in short time.
The content of the invention
The object of the present invention is to provide a kind of preparation method of Chinese fir seedling medium, using pig farm useless padding for pig sty and The leftover bits and pieces of bean jelly processing factory prepares Chinese fir seedling medium for primary raw material, belongs to the project that turns waste into wealth of waste material recycling, green Colour circle is protected;The matrix being prepared first plants edible mushroom, and Chinese fir seedling medium is re-used as after planting edible mushroom, and manufacturing cost is low, Realize the Multi-class propagation of waste resource, Chinese fir survival rate of seedling is high, and side fibrous root is flourishing, and the Chinese fir nursery stock cultivated is healthy and strong, improves The surrival rate of afforestation.
The present invention is realized using following technical scheme:
A kind of preparation method of Chinese fir seedling medium, preparation process comprise the following steps:
(1)Useless padding for pig sty 45-60 parts are taken by weight;20-40 parts of Chinese mesona herb slag;15-25 parts of manioc waste;Straw particle 10- 20 parts;5-10 parts of ramulus mori particle;5-8 parts of corn ear slag particle;3-5 parts of bagasse particle;
(2)Useless padding for pig sty, Chinese mesona herb slag, manioc waste, straw particle, ramulus mori particle, corn ear slag particle and bagasse is broken Grain is uniformly mixed and obtains premix;
(3)Add Mixed Microbes into premix, the weight ratio of premix and Mixed Microbes is 100:(0.5-2);Mixed Microbes are by withered grass Bacillus, Trichoderma viride and EM bacterium solutions composition, the weight ratio of bacillus subtilis, Trichoderma viride and EM bacterium solutions is 1:1:1;
(4)Premix and Mixed Microbes are put into tunnel fermentation kiln after mixing and are fermented, fermentation temperature is controlled at 25 DEG C -28 DEG C, aerobic fermentation 25-35 days;
(5)By the premix after fermentation when pasteurization 8-12 is small after, obtain culture material;Sterilisation temp is 58 DEG C -62 DEG C;
(6)Culture material after pasteurization, which is placed in edible fungus culturing canopy, is cooled to less than 25 DEG C, is implanted into edible fungi Kind, implantation edible fungus species need to be in the moistening soil of culture material upper table cover 4-5cm after 15 days;Moistening soil be using pond sludge, After yellow soil and soddy soil smash mixing, clear water is added to being sufficiently humidified so as to obtain the final product, the weight ratio of pond sludge, yellow soil and soddy soil For:3:2:(0.1-0.5), carry out edible fungus culturing;
(7)After edible mushroom has been cultivated, Chinese fir branches and leaves particle is added, is turned uniformly, it is more than week to place one, then turned over Heap, you can as masson pine seedling medium.
Chinese fir branches and leaves particle be the branch leaf leaf to be dropped using Chinese fir be crushed to the 0.5 following size in the inside obtain it is broken Grain;Chinese fir branches and leaves particle accounts for the 10-15% of seedling medium gross weight.
Multiple rows of blow vent is provided with the tunnel fermentation kiln ground, blow vent can be passed through compressed air, periodically to pre- Batch mixing carries out logical oxygen.The ground of tunnel fermentation kiln is hardening of cement ground, lays snorkel before hardened ground, and when hardening is left Blow vent, snorkel can discharge compressed gas from blow vent.The left side, the right side and the inside of tunnel fermentation kiln are partition wall, top surface It is provided with the sunshade that keeps off the rain.It after mixing, is sent out premix and Mixed Microbes by the culture medium of edible fungus of the present invention from tunnel Outwards stack on the ground, covered on blow vent inside ferment kiln, the compressed gas in snorkel is discharged from blow vent, for premix Material provides oxygen and keeps the temperature of premix fermentation.
Edible mushroom refers to the common edible mushroom such as White mushroom, oyster mushroom, coprinus comatus, needle mushroom and mushroom.
Chinese mesona herb (scientific name:Mesona chinensis BenthIt is) labiate, the lower portion of the stem is thrown oneself on the ground, and top is upright, Leaf is avette or ovate oval, and apex is slightly blunt, and base portion, which tapers, shortens handle into, and there is small sawtooth at edge, and two sides, which has to dredge, becomes mildewed;It is born in The leaf on inflorescence top is smaller, avette to up-side down triangle in bracteal, relatively spends short, the normal band lavender of base portion, as a result when come off.Always Locusta is weak, spends small, and verticillate, calyx is small, mitriform, and 2 lips, upper lip 3 is split, lower lip full edge, as a result when or tubular, under it is curved, have vertical Arteries and veins and horizontal wrinkle;Corolla pale red, upper lip is wealthy, full edge or fissure, lower lip oblong, depression;Stamen 4, filigree protrude;Gynoecium 1, style 2 is split;Floral disc is while expand.Pyrene ellipse.Autumn Mo at florescence.It is distributed in TaiWan, China, Zhejiang, Jiangxi, Guangdong, West Guangxi.Chinese mesona herb is a kind of important medical and edible dual purpose plant resource.Chinese mesona herb herb contains polysaccharide, relieves summer heat, is heat-clearing, cool Blood, function of detoxification.Its stem among the people of commonly using adds water to cook, then adds dilute starch that jelly (being commonly called as " bean jelly ") is made and eat, and is to relieve summer heat to quench one's thirst Splendid food.The Chinese mesona herb slag that the present invention uses is that bean jelly processing factory processes bean jelly, that is, mistake after adding water to cook using Chinese mesona herb Filter obtained Chinese mesona herb slag.Need to be dehydrated when the application is using Chinese mesona herb slag to water content could use for 50-70%.
Manioc waste, is the waste material of tapioca processing factory, almost without what use, factory is generally deposited in pond In, peasants are transported back the part material as fermentation fertilizer, and smelly pollution environment can only be allowed to by not using very much, but its Also containing part sugar, starch and vitamin and abundant organic matter, vitamin, starch and abundant organic matter therein It is a kind of comprehensive utilization well as the culture material of edible mushroom.The manioc waste that the present invention uses need to pass through drying, and water content is low In 20%.
Straw particle is to be ground into particle of the length less than 1 the inside using straw;The stem of straw rice, alias:The rice stalks of rice, wheat, etc., rice Ligusticumic, rice straw, straw, Latin literary fame:Caulis Et Folium Oryzae,Refer to the rice straw after threshing, can be used for when bavin burning, Thing can be tied up by weaving into rope form, or be made into " straw bag " as weaving one by one with machine, for being used to plant warming.Straw It is generally used to and feed ox, make the bed, lid thatched cottage, insulation vernalization, the lime juice of alkalescence can be hung after ashing to wash and starch clothing or lime juice processed Group etc..In today of development in science and technology, the plantation of rice is substantially entire mechanization, the cultivation area of China north and south rice, in water After rice harvesting, straw, which is substantially, abandons ability field, waits and dries rear burn and recycle in field.Burning straw is not only to waste material, most heavy What is wanted is the pollution for causing environment:Black, white, the smog of grey are drifted, diffused everywhere, severely impact people's health and Scenery it is clear.Form huge contrast is:This side paddy growing area is dark in the confused world that burning straw is done, the animal husbandry in a distant place Area's straw is the delicious food of livestock, very rare, or even high price is also difficult to buy.
Ramulus mori particle is to be ground into powder of the diameter less than 0.5 centimetre using ramulus mori;Ramulus mori, Classification system:Morus alba L, be the branches and leaves of mulberry tree, ramulus mori, mulberry shoot, RAMULUS MORI, general name.Machaka or dungarunga, high 3-15m.Bark is greyish white Color, has strip is shallow to split;Root skin yellowish-brown or reddish yellow, fibroid are strong.Single leaf alternate;The long l-2.5cm of petiole;Blade is avette or wide Avette, long 5-20cm, wide 4-10cm, apex is sharp sharp or tapering, and basal circular or near heart-shaped, there are rough sawn tooth or knuckle-tooth in edge, have When have irregular division, above it is hairless, it is glossy, below have undercoat on arteries and veins, hairiness between armpit, base goes out 3, arteries and veins and interweaves with thready pulse Reticulate, the back side is more apparent;Stipule lanceolar, it is caducous.
Corn ear slag particle is to be broken into particle of the granular size less than 1 the inside using corn ear ground-slag;Corn ear slag refers to It is corn ear remaining slag after threshing.
Bagasse particle is to be broken into particle of the length less than 1 the inside using bagasse powder;Bagasse is the pair of cane sugar factory Product, is all a large amount of wastes that resource is caused as fuel or paper making raw material all the time.The present invention uses bagasse as original Material prepares culture medium of edible fungus, belongs to the project that turns waste into wealth of leftover bits and pieces recycling, does not discharge waste residue externally, environmentally protective;It is sweet Contain substantial amounts of cellulose, the bagasse after everfermentation in bagasse, crude fibre can degrade, and crude protein can improve, it is sought Foster value is improved, and forms albumen and nutrient of the edible mushroom bacterium germination needed for growth.
For pigsty in terms of 100 square metres, the wood sawdust thickness being laid with pigsty is 30cm-50cm, with corn flour 50kg, After water 150kg and culture 10kg is stirred evenly, it is sprinkled upon on the wood sawdust laid, stands 3-5 days to wood sawdust bottom Portion's temperature up to after 45 DEG C, can pig raising in it, the waste material that raising is replaced after 3 years is useless padding for pig sty;Raising pigs When occurring wood sawdust in journey and occurring hardened, harrowed with rotary cultivator or instrument loose;When peculiar smell occurs in pigsty, with culture 5kg Add water 100kg to be sprinkled upon after mixing on wood sawdust, increase the biological inoculum of wood sawdust, it is had stronger degraded and is removed Smelly ability.
The culture, is to add strain, the urine water of pigsty discharge and the obtained training of the dry slag culture of pig manure using culture medium Thing is supported, culture incubation is to take weight ratio as 100:1-2:100:50 culture medium, strain, the urine water of pigsty discharge and pig Argol slag, quiescent culture is after 3-5 days, then the mixture for culture 3-5 days of learning from else's experience, and the urine water and the dry slag of pig manure of addition pigsty discharge are simultaneously It is uniformly mixed, the weight ratio of mixture, the urine water of pigsty discharge and the dry slag of pig manure is 3:1:0.5, after standing 4-6 days, trained Support thing.The culture medium, is made of the raw material of parts by weight:30-50 parts of wood sawdust, 30-50 parts of corn flour, molasses 5-10 Part, 1 part of copper sulphate, 3-5 parts of potassium dihydrogen phosphate, 1-2 parts of ferrous sulfate, 1.5 parts of zinc sulfate, 5-8 parts of urea, ammonium hydrogen carbonate 5-10 Part, 0.5-1 parts of eucalyptus oil.The strain, is made of the strain of parts by weight:1 part of saccharomycete, 1 part of bacillus subtilis, fiber Element decomposes 1 part of fungi, double 0.5 part of qi lactobacillus, 0.5 part of streptococcus thermophilus, 0.5 part of candidiasis.
The eucalyptus oil is the oil that fast-growing folium eucalypti is obtained with steam distillation.The ground such as Guangxi, Yunnan, Hainan are in recent years Substantial amounts of fast-growing eucalyptus is planted on barren hill, remaining branches and leaves are not assorted in addition to making fuel after timber is done in the felling of fast-growing eucalyptus Use, being scattered in rand can also pollute, and the distillating method of eucalyptus oil of the present invention is:Select fresh within cutting down 2 days Fast-growing folium eucalypti, is put into boiler, every 100 kilograms of fast-growing folium eucalyptis add 250-300 kilograms of water, and add 0.5-1 kilograms of market The family expenses liquid detergent of upper purchase, is put into water, boil distillation 2-3 it is small when, cooling, separation moisture content obtain eucalyptus oil, eucalyptus oil adds Enter in culture medium, make it have deodorization, sterilization and mosquito-repelling function.The cost of eucalyptus oil distillation is not also high.
Yeast is some unicellular fungis, Classification system:Yeast;The not unit of phylogeny classification.Be sac fungus, The common name of basidiomycetes Deng Ji sections unicellular fungi, produces, some is pathogenic bacteria, is genetic engineering and cell cycle available for brewing The model organism of research.Saccharomycete is the microorganism for being employed earliest in history of human civilization.It can survive in anaerobic environment.Mesh Yeast, can be divided into by the preceding known ability for having primary yeast more than 1000, spore (ascospore and basidiospore) being produced according to saccharomycete Three classes:The strain for forming spore belongs to sac fungus and basidiomycetes.Spore is not formed but mainly by gemmation come the title bred For fungi impertecti, or it is " false yeast " (class yeast).It is currently known few partial yeast and is classified into Ascomycota.Ferment Female bacterium is extensive in distributed in nature, is mainly grown in the moist border containing saccharide ring of slant acidity, and in wine brewing, it is also very heavy Will.
The bacillus subtilis, Classification system:Bacillussubtilis, be bacillus one kind.Withered grass 0.7~0.8 × 2~3 microns of bacillus individual cells, uniform coloring.Without pod membrane, peritrichous, can move.Gram-positive Bacterium, 0.6~0.9 × 1.0~1.5 microns of gemma, ellipse arrive column, central or slightly inclined positioned at thalline, and thalline is not after sporulation Expand.Bacterium colony rough surface is opaque, dirty white or yellowish, and when being grown in liquid medium within, wrinkle mould is commonly formed.Withered grass bud Spore bacillus has stronger protease, amylase and lipase active, can be active enzyme by internal activation of zymogen, can also divide - serial other enzymes are secreted, such as pectase, dextranase, cellulase, can help to decompose the thing such as non-starch polysaccharide in plant feed Matter, the present invention using bacillus subtilis decompose Chinese mesona herb slag, manioc waste, ramulus mori particle, straw particle, corn ear slag particle and The crude fibre of bagasse particle, forms albumen and nutrient of the edible mushroom bacterium germination needed for growth.
Cellulose decomposition fungiCellulose-decomposing f-ungiRefer to true with decomposition of cellulose ability Bacterium.Cellulose is difficult to be decomposed, it is known that in addition to fungi, only cellulose decomposing bacteria and a small number of mollusks can decompose it.
Streptococcus thermophilus, Classification system:Streptococcus thermophilus,Streptococcus thermophilus and lactobacillus buchneri All it is the essential material for making Yoghourt.Research show that this bacterium can only reach the first half of small intestine, rather than Bifidobacterium that Sample can go to large intestine.Scientist thinks Yoghourt bacterium living(Streptococcus thermophilus and lactobacillus buchneri)It can help lactose intolerance The people received digest lactose, because both bacterium can produce energy lactase.
Candidiasis, also known as candida albicans, (Candida Albicans) Latin literary fame:NostocSkin can be invaded, glued Film and internal organ, show as acute, subacute or chronic inflammation, are mostly secondary infection.Candidiasis species is very much, but energy What is caused a disease to people is only several, most common with candida albicans bacterium (C. albicans) i.e. Candida albicans, and pathogenicity is also most By force, it is secondly Candida tropicalis (C.tropicalis), it is other to also have monilia krusei bacterium (C. krusei), closely put down Sliding candidiasis (C. parapsilokis) and Candida pseudotropical (C. pseudotropicalis) etc..
Trichoderma viride(Trichoderma viride)One kind of Trichoderma, it is extensive in distributed in nature, often it is saprophytic in On timber, seed and plant residue.Trichoderma viride can produce a variety of biologically active enzyme systems, and institute's cellulase-producing activity is most One of high bacterial strain, caused cellulase have crop degradation, and effect is very good, and Trichoderma viride is a kind of resource again Abundant antagonistic microbe, have the function that in plant pathology biological control it is important, the present invention using Trichoderma viride coordinate it is withered Careless bacillus is decomposed in Chinese mesona herb slag, manioc waste, ramulus mori particle, straw particle, corn ear slag particle and bagasse particle Crude fibre, improves raw material availability.
EM bacterium(Effective Microorganisms)It is into EM bacterium are big by Japanese Ryukyu by about 80 kinds of microorganism groups Ratio is praised to be studied successfully professor's nineteen eighty-two according to husband, is put goods on the market the eighties.EM bacterium are with photosynthetic bacteria, lactic acid bacteria, yeast 10 based on bacterium and actinomyces belong to a kind of micro- life bacteria preparation that more than 80 microorganisms are combined.The mechanism of action is to form EM Bacterium and the competition of pathogenic microorganism contention nutrition, since em bacterium easily live and reproduce in the soil, so can it is very fast and stably Occupy the ecologic niche in soil, form the advantage group of beneficial microbial bacteria, thus control pathogenic microorganism breeding and Invasion and attack to crop.It is the developing direction of the ecological agriculture, is more advantageous to the sustainable development of agricultural.The beginning of the nineties at the end of the eighties, EM bacterium by the states such as Japan, Thailand, Brazil, the U.S., Indonesia, Sri Lanka be widely used in agricultural, cultivation, plantation, The fields such as environmental protection, achieve obvious economic benefit and ecological benefits.The present invention is determined culture material in fermentation process using EM fungi degradations The problem of easily being polluted by harmful bacteria, ensures that flowers grow up healthy and sound.
Above-mentioned strain is bought from strain in the market or cultivates to obtain by Guangxi Academy Of Sciences.
Chinese fir described herein refers to the China fir described in this natives of Guangxi, China fir(Scientific name:Cunninghamia lanceolata (Lamb.)Hook.)Also known as:Sha Mu, husky tree etc., belong to Coniferales, Taxodiaceae arbor, up to 30 meters, the diameter of a cross-section of a tree trunk 1.3 meters above the ground is reachable 2.5-3 rice;Bark taupe;Hibernaculum subcircular, male cone is coniform, female cone Dan Sheng, and cone oval is 2.5-5 centimetres long, 3-4 centimetres of footpath;Seed is flat, covers fruit scale, and long avette or square are circular, and in April at florescence, cone late October is ripe.Mainly may be used For wind-expelling pain-stopping, dissipate stasis of blood hemostasis.And building, bridge, shipbuilding, ore pillar, furniture etc..
The advantages of preparation method of Chinese fir seedling medium of the present invention is:
Culture medium of edible fungus is prepared for primary raw material in the useless padding for pig sty that the application uses pig farm to discharge, and belongs to waste material again The project that turns waste into wealth utilized, does not discharge waste residue externally, environmentally protective;The culture medium of edible fungus plantation edible mushroom being prepared goes out Mushroom is early, can adopt mushroom time length, and can reduce edible fungus culturing cost, improves edible fungus culturing economic benefit.Also with processing factory Culture medium of edible fungus is prepared with the leftover bits and pieces of tapioca processing factory, belongs to the item of turning waste into wealth of factory waste recycling Mesh, does not discharge waste residue externally, environmentally protective;The culture medium of edible fungus plantation edible mushroom being prepared is early, can adopt the mushroom time It is long, and edible fungus culturing cost can be reduced, edible fungus culturing economic benefit is improved, while solve bean jelly processing factory and para arrowroot The problem of powder processing factory discarded object, the production environment for having kept factory, achievees the purpose that factory and farmer's two-win.It can fill again Divide and utilize a large amount of crude fibres and organic matter contained in padding for pig sty, by adding mixed fermentation culture material, decompose crude fibre The nutritional ingredient of crude protein one kind is formed, promotes organic matter degradation, improves raw material availability.Chinese mesona herb herb contains polysaccharide, has and disappears Heat, heat-clearing, cool blood, function of detoxification, can shorten the time of edible mushroom bacterium germination and fruiting using Chinese mesona herb slag for culture material;Can be anti- Only disease occurs for edible mushroom, makes that insect prevention diseases prevention medicament need not be sprayed again during edible fungus culturing;The present invention uses one time fermentation Method, it is therefore an objective to as far as possible retain culture material in lignin and cellulose because appropriate cellulose and lignin has Promote the effect of edible mushroom bacterium germination and growth, culture medium of edible fungus nitrogen content is up to 2.3%, is especially advantageous for the growth of edible mushroom, makes Edible mushroom is from harvesting only needs 33 days or so are inoculated into, and cultivation cost is relatively low, hence it is evident that increase economic benefit.Recycle cultivation overfeeding By the use of the culture material of bacterium as Chinese fir seedling medium, the raw material sources of Chinese fir seedling medium are widened, reduce Chinese fir nursery production cost, Realize the Multi-class propagation of waste resource;Chinese fir branches and leaves particle is added using the culture material for cultivating edible mushroom, turns uniformly, places One more than week, then turning is carried out as Chinese fir seedling medium, plant percent is high, and side fibrous root is flourishing, the Chinese fir cultivated Nursery stock is healthy and strong, and late growth grows fine, and improves the surrival rate of afforestation.
Brief description of the drawings
Fig. 1 is the structure diagram for the tunnel fermentation kiln that the present invention uses.
Embodiment
With reference to embodiment, the technical solution in invention is clearly and completely described, described embodiment is only Only it is the part of the present invention, instead of all the embodiments.
Embodiment 1
Chinese fir seedling medium can be prepared into using following processing step:
(1)Useless padding for pig sty 45-60 parts are taken by weight;20-40 parts of Chinese mesona herb slag;15-25 parts of manioc waste;Straw particle 10- 20 parts;5-10 parts of ramulus mori particle;5-8 parts of corn ear slag particle;3-5 parts of bagasse particle;
(2)Useless padding for pig sty, Chinese mesona herb slag, manioc waste, straw particle, ramulus mori particle, corn ear slag particle and bagasse is broken Grain is uniformly mixed and obtains premix;
(3)Add Mixed Microbes into premix, the weight ratio of premix and Mixed Microbes is 100:(0.5-2);Mixed Microbes are by withered grass Bacillus, Trichoderma viride and EM bacterium solutions composition, the weight ratio of bacillus subtilis, Trichoderma viride and EM bacterium solutions is 1:1:1;
(4)Premix and Mixed Microbes are put into tunnel fermentation kiln after mixing and are fermented, fermentation temperature is controlled at 25 DEG C -28 DEG C, aerobic fermentation 25-35 days;
(5)By the premix after fermentation when pasteurization 8-12 is small after, obtain culture material;Sterilisation temp is 58 DEG C -62 DEG C;
(6)Culture material after pasteurization, which is placed in edible fungus culturing canopy, is cooled to less than 25 DEG C, is implanted into edible fungi Kind, implantation edible fungus species need to be in the moistening soil of culture material upper table cover 4-5cm after 15 days;Moistening soil be using pond sludge, After yellow soil and soddy soil smash mixing, clear water is added to being sufficiently humidified so as to obtain the final product, the weight ratio of pond sludge, yellow soil and soddy soil For:3:2:(0.1-0.5), carry out edible fungus culturing;
(7)After edible mushroom has been cultivated, Chinese fir branches and leaves particle is added, is turned uniformly, it is more than week to place one, then turned over Heap, you can as masson pine seedling medium.
Chinese fir branches and leaves particle be the branch leaf leaf to be dropped using Chinese fir be crushed to the 0.5 following size in the inside obtain it is broken Grain;Chinese fir branches and leaves particle accounts for the 10-15% of seedling medium gross weight.
Multiple rows of blow vent is provided with the tunnel fermentation kiln ground, blow vent can be passed through compressed air, periodically to pre- Batch mixing carries out logical oxygen.
The useless padding for pig sty is that the wood sawdust thickness being laid with pigsty is 30cm-50cm, with corn flour 50kg, After water 150kg and culture 10kg is stirred evenly, it is sprinkled upon on the wood sawdust laid, stands 3-5 days to wood sawdust bottom Portion's temperature up to after 45 DEG C, can pig raising in it, raise more than the 3 years waste materials replaced;Occurs wood during pig raising When sawdust occurs hardened, harrowed with rotary cultivator or instrument loose;When peculiar smell occurs in pigsty, water 100kg is added to mix with culture 5kg It is sprinkled upon after uniformly on wood sawdust.
The culture, is to add strain, the urine water of pigsty discharge and the obtained training of the dry slag culture of pig manure using culture medium Thing is supported, culture incubation is to take weight ratio as 100:1-2:100:50 culture medium, strain, the urine water of pigsty discharge and pig Argol slag, quiescent culture is after 3-5 days, then the mixture for culture 3-5 days of learning from else's experience, and the urine water and the dry slag of pig manure of addition pigsty discharge are simultaneously It is uniformly mixed, the weight ratio of mixture, the urine water of pigsty discharge and the dry slag of pig manure is 3:1:0.5, after standing 4-6 days, trained Support thing.
The culture medium, is made of the raw material of parts by weight:30-50 parts of wood sawdust, 30-50 parts of corn flour, molasses 5- 10 parts, 1 part of copper sulphate, 3-5 parts of potassium dihydrogen phosphate, 1-2 parts of ferrous sulfate, 1.5 parts of zinc sulfate, 5-8 parts of urea, ammonium hydrogen carbonate 5- 10 parts, 0.5-1 parts of eucalyptus oil.
The strain, is made of the strain of parts by weight:1 part of saccharomycete, 1 part of bacillus subtilis, cellulose decomposition fungi 1 Part, double 0.5 part of qi lactobacillus, 0.5 part of streptococcus thermophilus, 0.5 part of Candida.
Described above is not limitation of the present invention, and the present invention is also not limited to examples detailed above, the art it is general Logical technical staff, in the essential scope of the present invention, the variations, modifications, additions or substitutions made, should all belong to the guarantor of the present invention Protect scope.

Claims (7)

  1. A kind of 1. preparation method of Chinese fir seedling medium, it is characterised in that:Preparation process comprises the following steps:
    (1)Useless padding for pig sty 45-60 parts are taken by weight;20-40 parts of Chinese mesona herb slag;15-25 parts of manioc waste;Straw particle 10- 20 parts;5-10 parts of ramulus mori particle;5-8 parts of corn ear slag particle;3-5 parts of bagasse particle;
    (2)Useless padding for pig sty, Chinese mesona herb slag, manioc waste, straw particle, ramulus mori particle, corn ear slag particle and bagasse is broken Grain is uniformly mixed and obtains premix;
    (3)Add Mixed Microbes into premix, the weight ratio of premix and Mixed Microbes is 100:(0.5-2);Mixed Microbes are by withered grass Bacillus, Trichoderma viride and EM bacterium solutions composition, the weight ratio of bacillus subtilis, Trichoderma viride and EM bacterium solutions is 1:1:1;
    (4)Premix and Mixed Microbes are put into tunnel fermentation kiln after mixing and are fermented, fermentation temperature is controlled at 25 DEG C -28 DEG C, aerobic fermentation 25-35 days;
    (5)By the premix after fermentation when pasteurization 8-12 is small after, obtain culture material;Sterilisation temp is 58 DEG C -62 DEG C;
    (6)Culture material after pasteurization, which is placed in edible fungus culturing canopy, is cooled to less than 25 DEG C, is implanted into edible fungi Kind, implantation edible fungus species need to be in the moistening soil of culture material upper table cover 4-5cm after 15 days;Moistening soil be using pond sludge, After yellow soil and soddy soil smash mixing, clear water is added to being sufficiently humidified so as to obtain the final product, the weight ratio of pond sludge, yellow soil and soddy soil For:3:2:(0.1-0.5), carry out edible fungus culturing;
    (7)After edible mushroom has been cultivated, Chinese fir branches and leaves particle is added, is turned uniformly, it is more than week to place one, then turned over Heap, you can as masson pine seedling medium.
  2. 2. the preparation method of Chinese fir seedling medium according to claim 1, it is characterised in that:Chinese fir branches and leaves particle is to utilize The branch leaf leaf that Chinese fir drops is crushed to the particle that the 0.5 following size in the inside obtains;It is total that Chinese fir branches and leaves particle accounts for seedling medium The 10-15% of weight.
  3. 3. the preparation method of Chinese fir seedling medium according to claim 1, it is characterised in that:The tunnel fermentation kiln Multiple rows of blow vent is provided with face, blow vent can be passed through compressed air, and logical oxygen is periodically carried out to premix.
  4. 4. the preparation method of Chinese fir seedling medium according to claim 1, it is characterised in that:The useless padding for pig sty is The wood sawdust thickness being laid with pigsty is 30cm-50cm, is stirred with corn flour 50kg, water 150kg and culture 10kg equal It is even it is good after, be sprinkled upon on the wood sawdust laid, stand 3-5 days to wood sawdust bottom temp up to after 45 DEG C, can be in it Pig raising, raises more than the 3 years waste materials replaced;When occurring wood sawdust during pig raising and occurring hardened, with rotary cultivator or Instrument rake is loose;When peculiar smell occurs in pigsty, water 100kg is added to be sprinkled upon after mixing on wood sawdust with culture 5kg.
  5. 5. the preparation method of Chinese fir seedling medium according to claim 3, it is characterised in that:The culture, is profit The culture that the urine water and the dry slag culture of pig manure discharged with culture medium plus strain, pigsty obtain, culture incubation is to take weight Amount is than being 100:1-2:100:50 culture medium, strain, the urine water and the dry slag of pig manure of pigsty discharge, quiescent culture is after 3-5 days, then Learn from else's experience the culture mixture of 3-5 days, the urine water and the dry slag of pig manure for adding pigsty discharge are simultaneously uniformly mixed, and mixture, pigsty are discharged Urine water and the dry slag of pig manure weight ratio be 3:1:0.5, after standing 4-6 days, obtain culture.
  6. 6. the preparation method of Chinese fir seedling medium according to claim 5, it is characterised in that:The culture medium, by weight The raw material of amount part is made:30-50 parts of wood sawdust, 30-50 parts of corn flour, 5-10 parts of molasses, 1 part of copper sulphate, potassium dihydrogen phosphate 3-5 parts, 1-2 parts of ferrous sulfate, 1.5 parts of zinc sulfate, 5-8 parts of urea, 5-10 parts of ammonium hydrogen carbonate, 0.5-1 parts of eucalyptus oil.
  7. 7. the preparation method of Chinese fir seedling medium according to claim 5, it is characterised in that:The strain, by weight The strain composition of part:1 part of saccharomycete, 1 part of bacillus subtilis, 1 part of cellulose decomposition fungi, double 0.5 part of qi lactobacillus, thermophilic chain 0.5 part of coccus, 0.5 part of Candida.
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CN108934272A (en) * 2018-07-31 2018-12-07 广西壮族自治区林业科学研究院 A method of promote Jiangnan keteleeria fortunei seed to sprout
CN109089824A (en) * 2018-07-30 2018-12-28 广西武宣金泰丰农业科技发展有限公司 A kind of pueraria lobata plantation substrate
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CN109089827A (en) * 2018-07-30 2018-12-28 广西武宣金泰丰农业科技发展有限公司 A kind of potato plantation substrate
CN109169150A (en) * 2018-07-30 2019-01-11 广西武宣金泰丰农业科技发展有限公司 One cultivates peanut plantation substrate
CN109220700A (en) * 2018-07-30 2019-01-18 广西武宣金泰丰农业科技发展有限公司 A kind of passion fruit cuttage seeding plantation substrate

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