CN108934272A - A method of promote Jiangnan keteleeria fortunei seed to sprout - Google Patents
A method of promote Jiangnan keteleeria fortunei seed to sprout Download PDFInfo
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- CN108934272A CN108934272A CN201810857105.7A CN201810857105A CN108934272A CN 108934272 A CN108934272 A CN 108934272A CN 201810857105 A CN201810857105 A CN 201810857105A CN 108934272 A CN108934272 A CN 108934272A
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- 241000961578 Keteleeria fortunei Species 0.000 title claims abstract description 47
- 238000000034 method Methods 0.000 title claims abstract description 36
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 28
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 24
- 229930191978 Gibberellin Natural products 0.000 claims abstract description 15
- 239000003448 gibberellin Substances 0.000 claims abstract description 15
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical class C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 claims abstract description 15
- 239000012153 distilled water Substances 0.000 claims abstract description 9
- 238000011010 flushing procedure Methods 0.000 claims abstract description 9
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 36
- 238000005286 illumination Methods 0.000 claims description 26
- 102000004882 Lipase Human genes 0.000 claims description 11
- 108090001060 Lipase Proteins 0.000 claims description 11
- 239000004367 Lipase Substances 0.000 claims description 11
- 235000019421 lipase Nutrition 0.000 claims description 11
- 238000012545 processing Methods 0.000 claims description 11
- 230000001737 promoting effect Effects 0.000 claims 2
- 238000005406 washing Methods 0.000 claims 1
- 230000007226 seed germination Effects 0.000 abstract description 7
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 230000035784 germination Effects 0.000 description 27
- 238000002791 soaking Methods 0.000 description 10
- 238000012360 testing method Methods 0.000 description 8
- 238000005259 measurement Methods 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 241000218648 Keteleeria Species 0.000 description 2
- 241000218641 Pinaceae Species 0.000 description 2
- 235000011613 Pinus brutia Nutrition 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 230000035479 physiological effects, processes and functions Effects 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 241001499608 Keteleeria fortunei var. cyclolepis Species 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000005138 cryopreservation Methods 0.000 description 1
- 239000012297 crystallization seed Substances 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a kind of methods that promotion Jiangnan keteleeria fortunei seed is sprouted, it is the following steps are included: (1) chooses seeds: selected shape is ellipse, positive brown is glossy, the back side is faint yellow, the middle part of seed wing is wider or middle and lower part is wider, its mass of 1000 kernel is the seed of 130-155 g, then floatingly selects full seed；(2) it seed disinfection: is impregnated with the full seed that liquor potassic permanganate obtains seed selection, then uses distilled water flushing；(3) vernalization is handled: being that the seed after disinfection is impregnated 12-48 h by 50-300 mg/L Gibberellins solution under the conditions of temperature is 25-40 DEG C with concentration；(4) seed is sprouted: by vernalization, treated that seed is placed in the culture dish for being covered with 2 layers of filter paper, is then placed in culture in incubator.This method can significantly improve Jiangnan keteleeria fortunei percentage of seedgermination and seedling quality, shorten seed germinating time, provide a kind of reliable method of science for Jiangnan keteleeria fortunei large-scale planting and standardization production.
Description
Technical field
The invention belongs to field of plant growing technology, and in particular to a method of promote Jiangnan keteleeria fortunei seed to sprout.
Background technique
Jiangnan keteleeria fortunei (Keteleeria fortunei var. cyclolepis) is subordinate to Pinaceae (Pinaceae) Keteleeria
(Keteleeria), be the peculiar rare tree of one's native land in China, now only it is fragmentary it is scattered in China Fujian, it is Zhejiang, Jiangxi, Hunan, wide
The provinces and regions such as east, Guangxi, Yunnan, Guizhou.Jiangnan keteleeria fortunei trunk is tall and big logical straight, is thick with leaves dark green, and dense materials, wood color is beautiful,
Durable, tree root contains colloid, civil to be usually used in paper size, and therefore, Jiangnan keteleeria fortunei is a kind of of great value with material tree
Kind and afforestation ornamental tree species.Simultaneously as Jiangnan keteleeria fortunei tool resistance is strong, the good characteristics such as very fast, adaptable are grown,
Jiangnan keteleeria fortunei is excellent planting out tree species again.In recent years, due to artificially cutting down, own biological characteristic and natural environment
Etc. composite factors effect, Jiangnan keteleeria fortunei distribution reduces, and wild quantity sharply reduces, and is classified as local nurse tree by provinces and regions
Kind.
To alleviate the contradiction between shortage of resources and development and utilization, carrying out artificial cultivation is a kind of effective approach.Preceding
It is found in the investigation of Jiangnan keteleeria fortunei germplasm resource and breeding and seedling work of phase, the setting percentage of Jiangnan keteleeria fortunei population is lower, and seed loses
It educates phenomenon rate and is up to 80 %, and Jiangnan keteleeria fortunei germination percentage in seeding process is lower, germination is irregular, these situations seriously restrict
The standardized planting and production of Jiangnan keteleeria fortunei.On the other hand, due to the attributes of vegetation of different plants, seed physiology habit difference
Make the method for being suitble to its seed to sprout also different greatly, suitably facilitates the sprouting of Jiangnan keteleeria fortunei seed so there are no in the prior art
Method, especially for Jiangnan keteleeria fortunei.Therefore, to promote Jiangnan keteleeria fortunei large-scale planting and standardization production, research is shortened
The method of Jiangnan keteleeria fortunei seed germinating time, raising percentage of seedgermination and seedling quality is extremely urgent.
Summary of the invention
The present invention is directed to river for the deficiencies of Jiangnan keteleeria fortunei percentage of seedgermination is low in the prior art, germinating time is long
Southern keteleeria fortunei seed kind skin is hard, bad hydraulic permeability, rich grease-contained characteristic, provides a kind of side that promotion Jiangnan keteleeria fortunei seed is sprouted
Method promotes Jiangnan keteleeria fortunei seed to sprout, and shortens seed germinating time, improves percentage of seedgermination and seedling quality.
The present invention is achieved by the following technical scheme:
A method of promote Jiangnan keteleeria fortunei seed to sprout comprising following steps:
(1) choose seeds: selected shape be ellipse, positive brown is glossy, and the back side is faint yellow, the middle part of seed wing it is wider or under
Portion is wider, and mass of 1000 kernel is the seed of 130-155 g, it is mixed with clear water, and the empty grain of floating and flat is removed after being sufficiently stirred
Grain obtains full seed after then addition soaking with sodium hydroxide is washed by rubbing with the hands into water；
(2) seed disinfection: 10- is impregnated with the full seed that the liquor potassic permanganate that mass fraction is 0.2 % obtains seed selection
Then 15 min are used distilled water flushing 3-5 times；
(3) vernalization is handled: be 50-300 mg/L Gibberellins solution with concentration by the seed after disinfection being 25-40 DEG C of item in temperature
12-48 h is impregnated under part；
(4) seed is sprouted: by vernalization, treated that seed is placed in the culture dish for being covered with 2 layers of filter paper, is then placed in incubator,
It is 25 DEG C in temperature, humidity is 90 %, and the alternate illumination time is that 12 h illumination and 12 h are dark, and intensity of illumination is 1000 LX's
Under the conditions of, it is continuously cultivated using 24 h as a cycle.
It advanced optimizes, in above-mentioned steps (1) seed selection, 0.2 g sodium hydroxide and 1.5 U is added in every 100 ml clear water
Alkaline lipase washes seed by rubbing with the hands after impregnating 12h, the seed sunk to the bottom is pulled out to get full seed is arrived.
It advanced optimizes, above-mentioned steps (3) vernalization processing, is 200 mg/L Gibberellins solutions by the kind after disinfection with concentration
Son impregnates 48 h under the conditions of temperature is 30 DEG C.
The technical program has the advantages that compared with prior art
1, in the seed selection step of the method for the present invention, selected shape is ellipse, and positive brown is glossy, and the back side is faint yellow, kind
The middle part of wing is wider or the wider seed in middle and lower part, and the mass of 1000 kernel of seed is required to reach 130-155g.Wherein mass of 1000 kernel
It is to embody an important indicator of seed size and turgor, and measure the important evidence of seed quality.This method according to
The standard screenings seeds such as shape, color, seed wing shape and the mass of 1000 kernel of seed, can be effectively ensured screening obtain plumpness it is good,
The outstanding seed of quality improves percentage of seedgermination；Sodium hydroxide is added and seed is washed in alkaline lipase immersion by rubbing with the hands, can effectively go
Except a large amount of greases of kind of skin, while softening kind of a skin, and add the dosage that alkaline lipase also reduces sodium hydroxide, improves we
The safety of method.
2, in the vernalization processing step of the method for the present invention, for Jiangnan keteleeria fortunei seed kind skin, hard, bad hydraulic permeability, is rich in
Grease, the characteristic that germination is slow and germination percentage is low, using the seed after the Gibberellins solution soaking disinfection of suitable concentration, and science
Soaking temperature and time are controlled, not only promotes the germination of Jiangnan keteleeria fortunei seed, the germinating time of seed can also be shortened.
3, it is sprouted in step in the seed of the method for the present invention, seed is cultivated under 90% damp condition, guarantee Jiangnan keteleeria fortunei
Seed fully absorbs moisture when sprouting, and promotes its kind of skin expansion softening, increases oxygen and penetrate, the various physiology for reinforcing seed are living
It is dynamic, being absorbed and utilized for the nutriment stored in seed is improved, the germination percentage of seed is improved, shortens seed germinating time.
4, proving the method for the present invention not only according to experimental data can be improved the germination percentage for improving seed and shortens seed hair
Bud time, germinating energy, the plumule that can also improve Jiangnan keteleeria fortunei seed compared with prior art are long and radicle is long.It can be seen that
The present invention can General Promotion Seed Vitality, improve seedling quality.
5, the method for the present invention suitably facilitates the sprouting of Jiangnan keteleeria fortunei seed, easy to operate, at low cost, easy to spread, meets
The requirement of Jiangnan keteleeria fortunei large-scale planting and standardization production.
Specific embodiment
The present invention is further illustrated by the following examples, but not as limitation of the present invention.In the following example not
Dated specific experiment condition and method, used technological means are usually conventional hand well-known to those skilled in the art
Section.
Embodiment 1:
A method of promote Jiangnan keteleeria fortunei seed to sprout comprising following steps:
(1) choose seeds: selected shape be ellipse, positive brown is glossy, and the back side is faint yellow, the middle part of seed wing it is wider or under
Portion is wider, and mass of 1000 kernel is the seed of 130 g, it is mixed with clear water, the empty grain and empty grain of floating are removed after being sufficiently stirred, so
0.2 g sodium hydroxide and 1.5 U alkaline lipases is added in every 100 ml clear water afterwards, washes seed by rubbing with the hands after impregnating 12h, will sink to the bottom
Seed pull out to get to full seed；
(2) seed disinfection: 12 are impregnated with the full seed that the liquor potassic permanganate that mass fraction is 0.2 % obtains seed selection
Then min is used distilled water flushing 4 times；
(3) vernalization is handled: being that 200 mg/L Gibberellins solutions soak the seed after disinfection under the conditions of temperature is 30 DEG C with concentration
Steep 48 h；
(4) seed is sprouted: by vernalization, treated that seed is placed in the culture dish for being covered with 2 layers of filter paper, is then placed in incubator,
It is 25 DEG C in temperature, humidity is 90 %, and the alternate illumination time is that 12 h illumination and 12 h are dark, and intensity of illumination is 1000 LX's
Under the conditions of, it is continuously cultivated using 24 h as a cycle.
Embodiment 2:
A method of promote Jiangnan keteleeria fortunei seed to sprout comprising following steps:
(1) choose seeds: selected shape be ellipse, positive brown is glossy, and the back side is faint yellow, the middle part of seed wing it is wider or under
Portion is wider, and mass of 1000 kernel is the seed of 135 g, it is mixed with clear water, the empty grain and empty grain of floating are removed after being sufficiently stirred, so
0.2 g sodium hydroxide and 1.5 U alkaline lipases is added in every 100 ml clear water afterwards, washes seed by rubbing with the hands after impregnating 12h, will sink to the bottom
Seed pull out to get to full seed；
(2) seed disinfection: 10 are impregnated with the full seed that the liquor potassic permanganate that mass fraction is 0.2 % obtains seed selection
Then min is used distilled water flushing 3 times；
(3) vernalization is handled: being that 200 mg/L Gibberellins solutions soak the seed after disinfection under the conditions of temperature is 25 DEG C with concentration
Steep 36 h；
(4) seed is sprouted: by vernalization, treated that seed is placed in the culture dish for being covered with 2 layers of filter paper, is then placed in incubator,
It is 25 DEG C in temperature, humidity is 90 %, and the alternate illumination time is that 12 h illumination and 12 h are dark, and intensity of illumination is 1000 LX's
Under the conditions of, it is continuously cultivated using 24 h as a cycle.
Embodiment 3:
A method of promote Jiangnan keteleeria fortunei seed to sprout comprising following steps:
(1) choose seeds: selected shape be ellipse, positive brown is glossy, and the back side is faint yellow, the middle part of seed wing it is wider or under
Portion is wider, and mass of 1000 kernel is the seed of 155 g, it is mixed with clear water, the empty grain and empty grain of floating are removed after being sufficiently stirred, so
0.2 g sodium hydroxide and 1.5 U alkaline lipases is added in every 100 ml clear water afterwards, washes seed by rubbing with the hands after impregnating 12h, will sink to the bottom
Seed pull out to get to full seed；
(2) seed disinfection: 15 are impregnated with the full seed that the liquor potassic permanganate that mass fraction is 0.2 % obtains seed selection
Then min is used distilled water flushing 5 times；
(3) vernalization is handled: being that 100 mg/L Gibberellins solutions soak the seed after disinfection under the conditions of temperature is 35 DEG C with concentration
Steep 48 h；
(4) seed is sprouted: by vernalization, treated that seed is placed in the culture dish for being covered with 2 layers of filter paper, is then placed in incubator,
It is 25 DEG C in temperature, humidity is 90 %, and the alternate illumination time is that 12 h illumination and 12 h are dark, and intensity of illumination is 1000 LX's
Under the conditions of, it is continuously cultivated using 24 h as a cycle.
Embodiment 4:
A method of promote Jiangnan keteleeria fortunei seed to sprout comprising following steps:
(1) choose seeds: selected shape be ellipse, positive brown is glossy, and the back side is faint yellow, the middle part of seed wing it is wider or under
Portion is wider, and mass of 1000 kernel is the seed of 145 g, it is mixed with clear water, the empty grain and empty grain of floating are removed after being sufficiently stirred, so
0.2 g sodium hydroxide and 1.5 U alkaline lipases is added in every 100 ml clear water afterwards, washes seed by rubbing with the hands after impregnating 12h, will sink to the bottom
Seed pull out to get to full seed；
(2) seed disinfection: 13 are impregnated with the full seed that the liquor potassic permanganate that mass fraction is 0.2 % obtains seed selection
Then min is used distilled water flushing 4 times；
(3) vernalization is handled: being that 50 mg/L Gibberellins solutions impregnate the seed after disinfection under the conditions of temperature is 40 DEG C with concentration
48 h；
(4) seed is sprouted: by vernalization, treated that seed is placed in the culture dish for being covered with 2 layers of filter paper, is then placed in incubator,
It is 25 DEG C in temperature, humidity is 90 %, and the alternate illumination time is that 12 h illumination and 12 h are dark, and intensity of illumination is 1000 LX's
Under the conditions of, it is continuously cultivated using 24 h as a cycle.
Embodiment 5:
A method of promote Jiangnan keteleeria fortunei seed to sprout comprising following steps:
(1) choose seeds: selected shape be ellipse, positive brown is glossy, and the back side is faint yellow, the middle part of seed wing it is wider or under
Portion is wider, and mass of 1000 kernel is the seed of 140 g, it is mixed with clear water, the empty grain and empty grain of floating are removed after being sufficiently stirred, so
0.2 g sodium hydroxide and 1.5 U alkaline lipases is added in every 100 ml clear water afterwards, washes seed by rubbing with the hands after impregnating 12h, will sink to the bottom
Seed pull out to get to full seed；
(2) seed disinfection: 10 are impregnated with the full seed that the liquor potassic permanganate that mass fraction is 0.2 % obtains seed selection
Then min is used distilled water flushing 5 times；
(3) vernalization is handled: being that 200 mg/L Gibberellins solutions soak the seed after disinfection under the conditions of temperature is 35 DEG C with concentration
Steep 12 h；
(4) seed is sprouted: by vernalization, treated that seed is placed in the culture dish for being covered with 2 layers of filter paper, is then placed in incubator,
It is 25 DEG C in temperature, humidity is 90 %, and the alternate illumination time is that 12 h illumination and 12 h are dark, and intensity of illumination is 1000 LX's
Under the conditions of, it is continuously cultivated using 24 h as a cycle.
Embodiment 6:
A method of promote Jiangnan keteleeria fortunei seed to sprout comprising following steps:
(1) choose seeds: selected shape be ellipse, positive brown is glossy, and the back side is faint yellow, the middle part of seed wing it is wider or under
Portion is wider, and mass of 1000 kernel is the seed of 150g, it is mixed with clear water, the empty grain and empty grain of floating are removed after being sufficiently stirred, so
0.2 g sodium hydroxide and 1.5 U alkaline lipases is added in every 100 ml clear water afterwards, washes seed by rubbing with the hands after impregnating 12h, will sink to the bottom
Seed pull out to get to full seed；
(2) seed disinfection: 15 are impregnated with the full seed that the liquor potassic permanganate that mass fraction is 0.2 % obtains seed selection
Then min is used distilled water flushing 3 times；
(3) vernalization is handled: being that 300 mg/L Gibberellins solutions soak the seed after disinfection under the conditions of temperature is 35 DEG C with concentration
Steep 24 h；
(4) seed is sprouted: by vernalization, treated that seed is placed in the culture dish for being covered with 2 layers of filter paper, is then placed in incubator,
It is 25 DEG C in temperature, humidity is 90 %, and the alternate illumination time is that 12 h illumination and 12 h are dark, and intensity of illumination is 1000 LX's
Under the conditions of, it is continuously cultivated using 24 h as a cycle.
Test example:
Orthogonal test is designed, using Gibberellins solution concentration, soaking time, soaking temperature as factor, test measurement is in different vernalization
Under processing, germination percentage, germinating energy, germination index, germination time lag, the plumule of Jiangnan keteleeria fortunei seed are long, radicle is long.The specific step of test
It is rapid as follows:
(1) seed source: the Jiangnan keteleeria fortunei woods to grow fine in branch is clapped from Hezhou City great Gui mountain forest six for planting experimentally son.
Cone is acquired in November, 2015, cone is stacked into a couple of days indoors later, after seed in cone is sufficiently mature, is tedded in sun
Under light, deviates from naturally to seed, 4 DEG C of refrigerator cryo-conservations are put into after drying；
(2) seed selects: the seed choosing method described in accordance with the above-mentioned embodiment 1 chooses the full seed of test；
(3) seed disinfection: the seed disinfection method described in accordance with the above-mentioned embodiment 1 carries out seed disinfection；
(4) vernalization is handled: being urged before being sprouted using Gibberellins solution × soaking temperature × soaking time to Jiangnan keteleeria fortunei seed
Bud processing.Using Gibberellins solution concentration, soaking time, soaking temperature as factor, each factor 4 horizontal arrangement tests (table 1),
Totally 16 processing (table 2) are control (CK) with 12 h of clear water soaking at room temperature；
1 germination experiment factor level table of table
2 vernalization of table handles test combinations
(5) seed is sprouted: by vernalization, treated that seed is placed in the culture dish for being covered with 2 layers of filter paper, and each culture dish puts 30
Grain seed, each processing are repeated 3 times, and being put into temperature later is 25 DEG C, and humidity is 90 %, and intensity of illumination is 1000 LX, alternately
Light application time is to be cultivated in 12 h illumination/12 h dark incubator；
(6) seed sprouts observation: every 24 h is observed 1 time, is considered as germination when reaching seed length 1/2 with radicle, is persistently observed 30 d.
It counts percentage of seedgermination, germinating energy, germination index, germination time lag and plumule and radicle length etc. and sprouts index, each index meter
It calculates formula and is described as follows:
Germination percentage (%)=(chitting piece sum/for planting experimentally subnumber) × 100%；
Germinating energy (%)=(subnumber is planted experimentally in normal chitting piece number/confession in 10 d) × 100%；
Germination index=∑ (Gt/Dt), wherein Gt is the chitting piece number in t days, and Dt is germination number of days；
It germinates time lag (d): number of days needed for first seed is sprouted；
Plumule and radicle length (mm): after seed is sprouted, the seed for choosing 10 sproutings from each culture dish measures respectively
Plumule and radicle length (then all measurements less than 10)；
(7) measurement result: germination percentage, germinating energy, germination index, germination time lag, the plumule of Jiangnan keteleeria fortunei seed are long, radicle is grown
There is extremely significant difference (tables 3) under different vernalization processing；
The variance analysis and Multiple range test that seed is sprouted between the different vernalization processing of table 3
Note: " * * " is indicatedP=0.05 horizontal upper difference is extremely significant；The different letters of same column indicateP=0.05 horizontal upper significant difference,
Letter is identical, indicates that difference is not significant.
(8) conclusion: compared with contrast method, germination all substantially shortens method for treating seeds used in the present invention, hair
Bud gesture, germination index, plumule are long and radicle is long is better than control group, especially go to south China keteleeria fortunei germination in 10 conditions that handle
Rate, germinating energy, germination index, plumule are long, radicle is long maximum, respectively 83.33 %, the mm of 56.67 %, 28.32,20.05,
41.35 mm；20 have been respectively increased compared to the germination percentage of control (CK) processing, germinating energy, germination index, plumule length, radicle length
%,26.67 %,14.56 %,8.36 mm,9.77 mm；The germination time lag ratio control treatment of seed contracts under the conditions of processing 10 simultaneously
Short 3.66 d.It can thus be appreciated that the method for the present invention can significantly improve Jiangnan keteleeria fortunei percentage of seedgermination and seedling quality, shorten kind
Sub- germinating time, to promote Jiangnan keteleeria fortunei large-scale planting and standardization production to provide a kind of reliable method of science.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiments being understood that.
Claims (3)
1. it is a kind of promote Jiangnan keteleeria fortunei seed sprout method, it is characterised in that: itself the following steps are included:
Seed selection: selected shape is ellipse, and positive brown is glossy, and the back side is faint yellow, and the middle part of seed wing is wider or middle and lower part
Wider, mass of 1000 kernel is the seed of 130-155 g, it is mixed with clear water, the empty grain and empty grain of floating are removed after being sufficiently stirred,
Then sodium hydroxide is added into water and alkaline lipase impregnates after washing by rubbing with the hands, obtains full seed；
Seed disinfection: 10-15 is impregnated with the full seed that the liquor potassic permanganate that mass fraction is 0.2 % obtains seed selection
Then min is used distilled water flushing 3-5 times；
Vernalization processing: with concentration be 50-300 mg/L Gibberellins solution by the seed after disinfection under the conditions of temperature is 25-40 DEG C
Impregnate 12-48 h；
Seed is sprouted: by vernalization, treated that seed is placed in the culture dish for being covered with 2 layers of filter paper, is then placed in incubator,
Temperature is 25 DEG C, and humidity is 90 %, and the alternate illumination time is that 12 h illumination and 12 h are dark, and intensity of illumination is the item of 1000 LX
Under part, continuously cultivated using 24 h as a cycle.
2. a kind of method for promoting Jiangnan keteleeria fortunei seed to sprout according to claim 1, it is characterised in that: the step
It (3) is that the seed after disinfection is impregnated 48 h by 200 mg/L Gibberellins solutions under the conditions of temperature is 30 DEG C with concentration in.
3. a kind of method for promoting Jiangnan keteleeria fortunei seed to sprout according to claim 1, it is characterised in that: the step
(1) in, 0.2 g sodium hydroxide and 1.5 U alkaline lipases are added in every 100 ml clear water, washes seed by rubbing with the hands after impregnating 12h, it will
The seed sunk to the bottom is pulled out to arrive full seed.
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| CN201810857105.7A CN108934272B (en) | 2018-07-31 | 2018-07-31 | Method for promoting germination of seeds of sequoia jiangnanensis |
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| CN201810857105.7A CN108934272B (en) | 2018-07-31 | 2018-07-31 | Method for promoting germination of seeds of sequoia jiangnanensis |
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| CN108934272B CN108934272B (en) | 2021-01-29 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115777276A (en) * | 2022-11-24 | 2023-03-14 | 武汉市农业科学院 | Method for promoting germination of water hyacinth seeds |
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Application publication date: 20181207 Assignee: Guangxi Wuzhou Ningye Modern Agricultural Development Co.,Ltd. Assignor: GUANGXI ZHUANG AUTONOMOUS REGION FORESTRY Research Institute Contract record no.: X2023980046499 Denomination of invention: A Method for Promoting Seed Germination of Jiangnan Oil Fir Granted publication date: 20210129 License type: Common License Record date: 20231109 |
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