CN103621216A - Method used for shortening nursery stage of taxus mairei seeds - Google Patents
Method used for shortening nursery stage of taxus mairei seeds Download PDFInfo
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- CN103621216A CN103621216A CN201210300734.2A CN201210300734A CN103621216A CN 103621216 A CN103621216 A CN 103621216A CN 201210300734 A CN201210300734 A CN 201210300734A CN 103621216 A CN103621216 A CN 103621216A
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Abstract
The invention discloses a method used for shortening nursery stage of taxus mairei seeds. According to the method, taxus mairei seeds at a certain maturation stage are collected; accelerating germination is realized by subjecting the collected seeds to seed coat artificial delaminating, variable temperature laminated storage and hormone treatment; and when 25% of the collected seeds are sprouted, the collected seeds are sown. Normal germination of the collected taxus mairei seeds can be realized in a next spring, germination of the seeds using the method is one year earlier than that using existing taxus mairei seed germination technology, nursery stage is shortened from 18 months to 6 months, and time period needed by germination of taxus mairei seeds is shortened greatly.
Description
Technical field
The present invention relates to Chinese yew artificial breeding technique field, more specifically relate to a kind of method that Taxus chinensis var. mairei Seeds shortens growing-seedling period.
Background technology
Chinese yew be generally acknowledge in the world be on the brink of extinction rare plants for anticancer, and southern enqlish yew be Subtropic of China to one of warm temperate zone endemic tree, originating in areas to the south, China Yangtze river basin ， China is first class of protection plant.Chinese yew is advanced industrial and the engraving material of high-quality, graceful town and country Landscape Trees, good biopesticide preparation raw material, stem, branch, leaf, root can be used as medicine, main component, containing taxol, taxine, diterpenoids compound one-level Sciadopitysin, volatilization wet goods, is known as eucalyptus, plant giant panda.Integrate that medical value, afforestation value, biopesticide are worth, material is worth for high-quality, has high value of exploiting and utilizing.Wherein, taxol is described as one of anticancer drug of first choice in clinical application, and also just because of this, wild Chinese yew is very worthy, also constantly suffers artificial destruction, even endangered.
In the existing wild taxus resource of protection, artificial planting, breed Chinese yew, at present still the most effectively, the most practical means.But because Germination of Taxus mairei Seeds need be through the biological property in a summer of two winters, the periodicity that this has seriously restricted enterprise's artificial culture southern enqlish yew, has greatly restricted the development of this industry, cannot meet the demand of vast market to Chinese yew.
More prior art lays particular emphasis on and improves in Chinese yew seedling success rate and survival rate, for example Chinese patent application CN101347063A discloses a kind of method for breeding yew, that the Chinese yew seed that dries epidermis is washed by rubbing with the hands with washing clothes to kind of a point obfuscation grey, with clear water, rinse well, the warm water that is placed in 60～70 ℃ is processed after 10～30min, put into again wet sand and preserve, during to sowing time, carry out seed propagation.On earth's surface after planting, re-lay last layer faggot or curling leaf, and surface temperature is after planting remained on to 10～20 ℃, soil moisture conservation is 13～30%.The breeding method of this invention has solved Chinese yew seedling failure and the underground present situation of survival rate effectively, and emergence rate reaches more than 65%, and survival rate reaches more than 70%.Chinese patent application CN102415258A discloses the Chinese yew seed seedling-raising method that a kind of survival rate is higher, by the seed of gathering when fruit is peony, take the wet husky Tibetan lamination method stored seeds seed collecting vernalization step of 1 year of outdoor change naturally and the seed after preserving 1 year to have 30% seed breach current, sift out seed, be placed in 0.2% liquor potassic permanganate and sterilize 10 minutes, with clear water, rinse well and dry again, selecting in the sowing not of garden of time in early spring sowing.Seedling-cultivating method of the present invention meets Chinese yew under field conditions (factors) as far as possible, the best natural conditions of seminal propagation, stratification growth and raising survival rate.
Prior art only lays particular emphasis on how stratification is grown and improves survival rate, but the required time cycle of Taxus chinensis var. mairei Seeds rudiment still needs a summer of two winters.Therefore how breaking Taxus chinensis var. mairei Seeds after-ripening and the long biological property of dormancy time, shorten the Taxus chinensis var. mairei Seeds required time cycle of rudiment, is a technical problem of being badly in need of solution.
Summary of the invention
The object of the present invention is to provide a kind of Taxus chinensis var. mairei Seeds to shorten the method for growing-seedling period, the seed Second Year of Chinese yew First Year collection is normally sprouted spring, than existing Germination of Taxus mairei Seeds technology, carry the previous year, seedling raise period shortened to 6 months by former 18 months.
To achieve these goals, the present invention adopts following technical scheme:
Taxus chinensis var. mairei Seeds shortens a method for growing-seedling period, it comprise by the seed of collection through artificial technology's delamination, the alternating temperature stratification of kind of skin preserve, vernalization, to the step that has 25% seed germination to sow, is characterized in that after HORMONE TREATMENT:
1) plant artificial technology's delamination of skin, seed and ash are mixed by a certain percentage, pack sealing 1 day, then together with sack immersion, after the delamination of kind skin, the grey mixture of kind is poured in sieve and cleaned with clear water, remove covering of a seed and ash;
2) alternating temperature stratification is preserved, step 1) seed that obtains after 1 day, puts into dustpan with emerge in worm water, every day, shading after 3 days, the husky Tibetan of digging pit were stored sooner or later by each sprinkling of warm water 1 time, and stir up and down once noon every day, and drench a small amount of warm water, until take out March next year;
3) HORMONE TREATMENT and vernalization, by step 2) seed that takes out is with alcohol and warm water mixed liquor seed soaking half an hour, pulls the certain density gibberellin seed soaking of rear use 1 day out, with breaking dormancy, improves emergence rate, through vernalization in approximately 10 days, approximately there is 25% seed germination to sow.
Described step 1) seed and ash described in mix by 1: 10 mass ratio.
Described step 3) alcohol described in and warm water mixed liquor mix by 1: 2 volume ratio.
Described step 3) described in, gibberellin concentration is not less than 100mg/L.
Described step 2) and step 3) described in the temperature of warm water be 40～45 ℃.
The acquisition time of described seed is mid or late October, the seed of gathering when fruit is peony.
According to the present invention, Taxus chinensis var. mairei Seeds shortens the method for growing-seedling period, artificial technology's delamination, alternating temperature stratification preservation, HORMONE TREATMENT and vernalization through kind of skin, the seed Second Year of Chinese yew First Year collection is normally sprouted spring, than existing Germination of Taxus mairei Seeds technology, carry the previous year, seedling raise period shortened to 6 months by former 18 months, had shortened widely the required time cycle of Taxus chinensis var. mairei Seeds rudiment.
Embodiment
Below in conjunction with specific embodiment, the present invention is done to further expansion explanation, but it is pointed out that the method for Taxus chinensis var. mairei Seeds shortening growing-seedling period of the present invention is not limited to this concrete treatment step.Obviously be understandable that for those skilled in the art, even if the following description content does not make any adjustments or revises, also can be directly applied at these unspecified other similar method and treatment steps.
A kind of Taxus chinensis var. mairei Seeds shortens the method for growing-seedling period, it comprise by the seed of collection through artificial technology's delamination of kind of skin, alternating temperature stratification preserve, after HORMONE TREATMENT vernalization to the step that has 25% seed germination to sow, wherein: 1) seed and ash are mixed by a certain percentage, pack sealing 1 day, then together with sack, soak, after planting skin delamination, the grey mixture of kind is poured in sieve and cleaned with clear water, remove covering of a seed and ash; 2) step 1) seed that obtains after 1 day, puts into dustpan with emerge in worm water, and every day, shading after 3 days, the husky Tibetan of digging pit were stored sooner or later by each sprinkling of warm water 1 time, and stir once noon every day up and down, and drench a small amount of warm water, until take out March next year; 3) by step 2) seed that takes out is with alcohol and warm water mixed liquor seed soaking half an hour, pulls the certain density gibberellin seed soaking of rear use 1 day out, with breaking dormancy, improves emergence rate, through vernalization in approximately 10 days, approximately has 25% seed germination to sow.Plant the mode of skin delamination without any restriction; such as some are artificial or the technology of mechanical delamination or method; this is all well-known to those skilled in the art; be understandable that; as long as can be by kind of a skin delamination; no matter adopt which kind of technological means, all can meet the present invention, also all within protection scope of the present invention.
Wherein, seed and ash step 1) mix by 1: 10 mass ratio; Step 3) alcohol described in and warm water mixed liquor mix by 1: 2 volume ratio; Step 3) concentration of gibberellin described in is not less than 100mg/L, not higher than 1000mg/L; Step 2) and step 3) described in the temperature of warm water be 40～45 ℃; The acquisition time of seed is mid or late October, the seed of gathering when fruit is peony.Be understandable that, these mixtures or mixed liquor proportioning, concentration etc. are not limited to this specific fixed value, and descending unsteady certain limit thereon and can realize corresponding effect also should be within protection scope of the present invention.
Seedbed before planting seed arranges link can be referring to prior art, for example preparing seedbed step is: become seedbed (3 row consolidation in booth the end of the year, long 30 meters of every row, wide 1.2 meters, height is 15 centimetres), with plate, make real (preventing length under seedling root, convenient transplanting later), laying element is husky above, thick approximately 10 centimetres.Basic unit paves and with carbendazol, sparges bed surface afterwards, sealing a period of time.Wherein mainly hold the allotment of medium of seedling bed, meet the needs of seed and seedling growth.For example, when next year, sowed for March, Nutrition Soil replacement upper berth, the seedbed element sand that sawdust, chemical fertilizer, rural area soil etc. mix is by a certain percentage put in employing in polypots body, the germination rate that can effectively improve seed reaches 85%, survival rate is 80%, and germination rate and the survival rate of seed only has respectively 45% and 28% while adopting upper berth, seedbed element husky.
Sowing step can be referring to prior art, for example sowing in March next year, (can not be too much when having 25% seed breach now white, during sowing, prevent from touching disconnected plumule), sift out in time seed and be placed in carbendazim solution and sterilize a few minutes, then rinse well with clear water, after drying open fire, uniform broadcasting is on seedbed, will cover laminin sand or a Nutrition Soil above, thickness is advisable to lose seed, waters permeable overlay film (sprinkling irrigation).
Embodiment 1
Chinese yew seed collecting Best Times is mid-October, the seed of gathering when fruit is peony.After screening, seed and ash are mixed in the ratio of 1: 10, pack Nylon Bag sealing 1 day into, then connect after bag immersion, wear galosh and step on, step on and planting grey mixture, pour in sieve and clean with clear water afterwards, remove covering of a seed and ash.
The aforementioned seed of kind of skin that goes after one day, is put into dustpan with the emerge in worm water of 45 ℃, sooner or later use each sprinkling of warm water 1 time of 40 ℃ every day, shading after 3 days, the sand of digging pit store, and stir once noon every day up and down, and drench a small amount of water, it is heated evenly, is conducive to germinate.
Take out March next year, with alcohol and 45 ℃ of warm water (volume ratio 1: 2) mixed liquor, soaks seed half an hour, pulls the rear seed soaking of the gibberellin by 100mg/L concentration 1 day out, with breaking dormancy, improves emergence rate.Approximately, through vernalization in 10 days, approximately there is 25% seed germination, can sow.So, just sprouting time can be shortened to 6 months from original 18 months, shorten widely the required time cycle of Taxus chinensis var. mairei Seeds rudiment.
Embodiment 2
Chinese yew seed collecting Best Times is late October, the seed of gathering when fruit is peony.After screening, seed and ash are mixed in the ratio of 1: 10, pack Nylon Bag sealing 1 day into, then connect after bag immersion, wear galosh and step on, step on and planting grey mixture, pour in sieve and clean with clear water afterwards, remove covering of a seed and ash.
The aforementioned seed of kind of skin that goes after one day, is put into dustpan with the emerge in worm water of 40 ℃, sooner or later use each sprinkling of warm water 1 time of 45 ℃ every day, shading after 3 days, the sand of digging pit store, and stir once noon every day up and down, and drench a small amount of water, it is heated evenly, is conducive to germinate.
Take out March next year, with alcohol and 40 ℃ of warm water (1: 2 volume ratio) mixed liquor, soaks seed half an hour, pulls the rear seed soaking of the gibberellin by 500mg/L concentration 1 day out, with breaking dormancy, improves emergence rate.Approximately, through vernalization in 10 days, approximately there is 25% seed germination, can sow.So, just sprouting time can be shortened to 6 months from original 18 months, shorten widely the required time cycle of Taxus chinensis var. mairei Seeds rudiment.
Although above the specific embodiment of the present invention has been given to describe in detail and explanation; but what should indicate is; we can carry out various equivalences to above-mentioned embodiment according to conception of the present invention and change and revise; when its function producing does not exceed spiritual that specification contains yet, all should be within protection scope of the present invention.
Claims (6)
1. Taxus chinensis var. mairei Seeds shortens a method for growing-seedling period, it comprise by the seed of collection through artificial technology's delamination, the alternating temperature stratification of kind of skin preserve, vernalization, to the step that has 25% seed germination to sow, is characterized in that after HORMONE TREATMENT:
1) plant artificial technology's delamination of skin, seed and ash are mixed by a certain percentage, pack sealing 1 day, then together with sack immersion, after the delamination of kind skin, the grey mixture of kind is poured in sieve and cleaned with clear water, remove covering of a seed and ash;
2) alternating temperature stratification is preserved, step 1) seed that obtains after 1 day, puts into dustpan with emerge in worm water, every day, shading after 3 days, the husky Tibetan of digging pit were stored sooner or later by each sprinkling of warm water 1 time, and stir up and down once noon every day, and drench a small amount of warm water, until take out March next year;
3) HORMONE TREATMENT and vernalization, by step 2) seed that takes out is with alcohol and warm water mixed liquor seed soaking half an hour, pulls the certain density gibberellin seed soaking of rear use 1 day out, with breaking dormancy, improves emergence rate, through vernalization in approximately 10 days, approximately there is 25% seed germination to sow.
2. Taxus chinensis var. mairei Seeds according to claim 1 shortens the method for growing-seedling period, it is characterized in that step 1) described in seed and ash by 1: 10 mass ratio, mix.
3. Taxus chinensis var. mairei Seeds according to claim 1 shortens the method for growing-seedling period, it is characterized in that step 3) described in alcohol and warm water mixed liquor by 1: 2 volume ratio, mix.
4. Taxus chinensis var. mairei Seeds according to claim 1 shortens the method for growing-seedling period, it is characterized in that step 3) described in gibberellin concentration be not less than 100mg/L.
5. Taxus chinensis var. mairei Seeds according to claim 1 shortens the method for growing-seedling period, it is characterized in that step 2) and step 3) described in the temperature of warm water be 40～45 ℃.
6. Taxus chinensis var. mairei Seeds according to claim 1 shortens the method for growing-seedling period, it is characterized in that the acquisition time of described seed is mid or late October, the seed of gathering when fruit is peony.
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| CN201210300734.2A CN103621216A (en) | 2012-08-23 | 2012-08-23 | Method used for shortening nursery stage of taxus mairei seeds |
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| CN201210300734.2A CN103621216A (en) | 2012-08-23 | 2012-08-23 | Method used for shortening nursery stage of taxus mairei seeds |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104380876A (en) * | 2014-10-31 | 2015-03-04 | 江苏红豆杉生物科技股份有限公司 | Pregermination method of taxus mairei seeds |
| CN104380877A (en) * | 2014-12-09 | 2015-03-04 | 融安县良丰种苗有限责任公司 | Taxus chinensis seed treatment method |
| CN108522120A (en) * | 2018-04-14 | 2018-09-14 | 浙江新景市政园林有限公司 | The breeding method of Chinese yew |
| CN108934272A (en) * | 2018-07-31 | 2018-12-07 | 广西壮族自治区林业科学研究院 | A method of promote Jiangnan keteleeria fortunei seed to sprout |
| CN108934268A (en) * | 2018-07-03 | 2018-12-07 | 东北林业大学 | A method of abolishing Chinese yew seed dormancy |
| CN109819835A (en) * | 2019-03-28 | 2019-05-31 | 曹德 | A kind of breeding method of Qinling Mountains indigo plant ice Chinese fir |
| CN111527829A (en) * | 2020-06-04 | 2020-08-14 | 中国科学院昆明植物研究所 | Method for promoting germination of taxus chinensis seeds |
| CN115152354A (en) * | 2022-07-27 | 2022-10-11 | 中国长江三峡集团有限公司 | Method for efficiently breeding precious medicinal plant taxus chinensis |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104380876A (en) * | 2014-10-31 | 2015-03-04 | 江苏红豆杉生物科技股份有限公司 | Pregermination method of taxus mairei seeds |
| CN104380877A (en) * | 2014-12-09 | 2015-03-04 | 融安县良丰种苗有限责任公司 | Taxus chinensis seed treatment method |
| CN104380877B (en) * | 2014-12-09 | 2016-08-24 | 融安县良丰种苗有限责任公司 | Ramulus et folium taxi cuspidatae method for treating seeds |
| CN108522120A (en) * | 2018-04-14 | 2018-09-14 | 浙江新景市政园林有限公司 | The breeding method of Chinese yew |
| CN108934268A (en) * | 2018-07-03 | 2018-12-07 | 东北林业大学 | A method of abolishing Chinese yew seed dormancy |
| CN108934272A (en) * | 2018-07-31 | 2018-12-07 | 广西壮族自治区林业科学研究院 | A method of promote Jiangnan keteleeria fortunei seed to sprout |
| CN108934272B (en) * | 2018-07-31 | 2021-01-29 | 广西壮族自治区林业科学研究院 | Method for promoting germination of seeds of sequoia jiangnanensis |
| CN109819835A (en) * | 2019-03-28 | 2019-05-31 | 曹德 | A kind of breeding method of Qinling Mountains indigo plant ice Chinese fir |
| CN111527829A (en) * | 2020-06-04 | 2020-08-14 | 中国科学院昆明植物研究所 | Method for promoting germination of taxus chinensis seeds |
| CN115152354A (en) * | 2022-07-27 | 2022-10-11 | 中国长江三峡集团有限公司 | Method for efficiently breeding precious medicinal plant taxus chinensis |
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Application publication date: 20140312 |