CN109220060A - A kind of rhizoma polygonati seed seedling-raising method - Google Patents
A kind of rhizoma polygonati seed seedling-raising method Download PDFInfo
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- CN109220060A CN109220060A CN201811370755.5A CN201811370755A CN109220060A CN 109220060 A CN109220060 A CN 109220060A CN 201811370755 A CN201811370755 A CN 201811370755A CN 109220060 A CN109220060 A CN 109220060A
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- rhizoma polygonati
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
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Abstract
The present invention proposes a kind of rhizoma polygonati seed seedling-raising method, includes the following steps: selection, immersion, fine sand processing, storage, vernalization, sowing and seedling period management；The present invention first carries out soaking fermentation to rhizoma polygonati seed, then it is refrigerated on culture medium again, last breeding in the soil, it is substantially shorter the hibernation period of rhizoma polygonati seed, and then shorten breeding process, reproductive process is simple, a large amount of rhizoma polygonati seedlings can be quickly provided, reducing the breeding of rhizoma polygonati stem tuber reduces planting cost, promotes rhizoma polygonati artificial growth, reduces the excavation of wild rhizoma polygonati, improve the income of farmer, and by using mating system of the invention, the germination percentage of finally obtained rhizoma polygonati seed is also higher, is conducive to promote.
Description
Technical field
The present invention relates to a kind of rhizoma polygonati seed seedling-raising methods.
Background technique
A kind of integration of drinking and medicinal herbs of rhizoma polygonati (rhizomapolygonti), Liliaceae, Polygonatum (polygonatum) is planted
Object.With block, big, Fei Run, color be yellowish-white, the transparent person of section be it is excellent, bitter person can not be used as medicine.The Shennong's Herbal of Han dynasty, the Ming Dynasty
" the Qingyang county annals " of Compendium of Material Medica and the Qing Dynasty have the record of its herbal cuisine effect.Rhizoma polygonati have boosting qi and nourishing yin, invigorating the spleen, moistening lung,
The functions such as kidney-nourishing.It is weakness of the spleen and the stomach for treating, fatigue and asthenia, dry deficiency of food, deficiency syndrome of the lung cough caused by dryness, asthenia of essence and blood, the diseases such as Heat Diabetes
And it is also very effective in cure to diabetes.It is existing wherein containing several functions compositions such as Siberian solomonseal rhizome polysaccharide, saponin(e, amino acid and cardiac glycosides
Generation studies have shown that Siberian solomonseal rhizome polysaccharide have anti-aging, reducing blood lipid preventing arteriosclerosis, it is hypoglycemic, improve and improve memory, it is antitumor,
Adjust the effects of immune, antiviral.Sibiricoside has improvement memory, antibacterial, antitumor, adjusting blood glucose etc. are functional to make
With.For rhizoma polygonati as medicinal, ornamental, beauty and edible plant is integrated, economic value is huge, has a extensive future.With
Market demand it is growing day by day, wild resource has been far from satisfying the demand of people, in addition exhaustive exploitation, causes wild money
Source is fewer and fewer.
Traditional planting rhizoma polygonati is to belong to vegetative propagation using the young plant under cutting out from rhizoma polygonati stem tuber top.And long-term nothing
Sexual reproduction causes rhizoma polygonati quality deterioration, and pest and disease damage problem is got worse, serious to limit the production scale of rhizoma polygonati.And rhizoma polygonati kind
Sub then habit there are embryo after-ripening, germination percentage is lower under natural conditions.With reaching its maturity for tissue culture technology, pass through tissue-culturing rapid propagation
Mode cultivate rhizoma polygonati seedling and become a kind of feasible road.Traditional cultivation is high in addition to investment threshold, at high cost outer, such soil
Ground be also it is extremely rare, not only cost be in rapid increase trend, to realize scale plant, the overwhelming majority area be almost nothings
Ground is optional.On the other hand, non-staple food grain producing region, especially backward area, stay-at-home women, old man, which have become, is mainly engaged in plantation
The labour of industry, labour quality and be suitable for that the intensity of labour all decreases sharply, it is also difficult to adapt to traditional rhizoma polygonati and deep-cut and rhizoma polygonati
Harvesting.
The development of traditional rhizoma polygonati always exists large labor intensity, and especially excavation difficulty is big, high production cost, finished product one
The problem of cause property difference；Selection of land condition is harsh, and optional land used is rare, and corresponding cost of land height is also an important factor for restricting development；
Quickly go up in the price of labour power, staple food grain land used is increasingly well sold and in short supply, and market is further harsh to the consistency of rhizoma polygonati finished product works as
Under, how to realize that the low labor intensity production rhizoma polygonati of low cost becomes further important.
Since the rhizoma polygonati seed seedling-raising time is long, low reproduction rate, the factors such as more difficult collection of seed cause artificial large area to be planted
Cost greatly increase, however in traditional artificial growth, vegetative propagation is mainly utilized, using Propagation of Rhizomes, mostly generation breeding
After degenerate relatively serious, easily infected virus, in addition, but can consume rhizoma polygonati seed a large amount of nutriment during sand storage,
When being unfavorable for seed sowing, the germination of seed, along with the breeding management for lacking fining, so that the germination percentage of seed only has
60% or so, therefore, the present invention proposes a kind of rhizoma polygonati seed seedling-raising method place so as to solve the deficiencies in the prior art.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of rhizoma polygonati seed seedling-raising methods.
The technical scheme is that a kind of rhizoma polygonati seed seedling-raising method, it is characterised in that the following steps are included:
A. it chooses:
In the rhizoma polygonati seed that or so 10-11 month harvesting is mature, wherein picking out the rhizoma polygonati seed of full grains, fruit is gone in stranding
Skin；
B. it impregnates:
Rhizoma polygonati seed in step a is placed in soak after immersion treatment 2-3min, is taken out, rinses two with clear water
Time, wherein soak is made of following material: citric acid 15-19 parts by weight, 1,2-diaminocyclohexane tetraacetic acid 10-13 parts by weight, water
100-120 parts by weight；
C. fine sand is handled:
By the ABT of 5-10mg/L take root amidin, the indolebutyric acid aqueous solution of 10-20mg/L, 100-200mg/L it is more
Bacterium Lingshui Spring solution is uniformly mixed in equal volume, is mixed fine sand with gained mixed liquor wet, and humid control is in 30-50%；
D. it stores:
Shady place storage is placed on after fine sand mixes with 1:3 by treated in rhizoma polygonati seed and step c, temperature maintains 1-
7℃；
E. vernalization:
The seed that the storage processing time is 90-100 days is layered on culture medium, then covers one layer of culture medium above,
Vernalization under 23-27 DEG C of indoor conditions；
F. it sows:
It selects place as nursery, the seed drilling through step e vernalization on ready seedling bed, sows rear cover
Upper withered grass is simultaneously watered, and finally covers shading net again；
G. seedling period management:
After emerging, withered grass and shading net are thrown off, shading net is taken on seedling bed, keeps seedling bed wet, gives seedling
Timely weeding and apply farmyard manure strong sprout.
A kind of rhizoma polygonati seed seedling-raising method of the present invention, it is characterised in that: harvesting maturation 10-11 month in the step a
Rhizoma polygonati seed, wherein pick out the rhizoma polygonati seed of full grains, pericarp is removed in stranding, is cleaned with clear water, seed is placed in 30-40 DEG C
It is impregnated in strong tea for 24 hours, taking-up is dried.
A kind of rhizoma polygonati seed seedling-raising method of the present invention, it is characterised in that: first make soak before being impregnated in the step b
It is good, 15 parts by weight citric acids and part water are poured into stirred tank and stir 5min, then by 10 parts by weight 1,2-diaminocyclohexane tetraacetic acids and
Remaining water pours into stirred tank, continues to stir 5min, rear to take out, and the rhizoma polygonati seed in step a is then placed on immersion
In liquid after immersion treatment 2-3min, takes out, be rinsed with water twice.
A kind of rhizoma polygonati seed seedling-raising method of the present invention, it is characterised in that: first by the ABT root-inducing powder of 5mg/L in the step c
Aqueous solution and the indolebutyric acid aqueous solution of 10mg/L pour into and stir 5min in stirred tank, then by the carbendazim aqueous solution of 100mg/L
It pours into stirred tank, continues to stir 5min, rear to take out, finally the mixed liquor obtained by is mixed fine sand wet, and humid control is in 30-
50%.
A kind of rhizoma polygonati seed seedling-raising method of the present invention, it is characterised in that: rhizoma polygonati seed is admixed into step c in the step d
In in treated fine sand, per cubic centimeter mix admixes 4-8 rhizoma polygonati seeds in wet fine sand, be subsequently placed in and store shady and coolly,
Periodically watering keeps humidity 30-50%, stores 3-4 months.
A kind of rhizoma polygonati seed seedling-raising method of the present invention, it is characterised in that: the culture medium in the step e is through everfermentation
The mixing of one or more of rice chaff, decomposed leaves or other loose nutrient matrixes.
A kind of rhizoma polygonati seed seedling-raising method of the present invention, it is characterised in that: by the rhizoma polygonati after step d storage in the step e
Seed and fine sand are uniformly sowed on culture medium together, are sowed with a thickness of 0.5-1cm, then sow one layer of culture again above
Base forms the high ridge of width 80-100cm, high 7-10cm, and ridge spacing is 15-25cm, while the holding culture that also to water sooner or later daily
Base humidity 30-50%.
A kind of rhizoma polygonati seed seedling-raising method of the present invention, it is characterised in that: press line-spacing 15cm in the step f on seedling bed
Ditch depth 3-5cm, and the seed through step 5 vernalization is uniformly sown into ditch, and thickness of earth covering 2.5-3cm is slightly jammed on, and keeps soil
Earth moistens, and after planting burning is primary permeable, then inserts and encircles item, fastener agricultural film, reinforcement arched shed seedbed management, timely ventilation, hardening,
When equal heights of seedling 3cm, spacious night in daytime is covered, and gradually removes arched shed.
A kind of rhizoma polygonati seed seedling-raising method of the present invention, it is characterised in that: after planting the soil moisture is not less than 15 to the step f
DEG C, a water is poured soil every other week, and irrigation amount, which is subject to, to be impregnated within topsoil 2cm, and the environment of seedling raising process is positive
Normal atmospheric environment will apply an organic fertilizer every two weeks with time-histories, and each dose is no less than 4kg/ ㎡.
A kind of rhizoma polygonati seed seedling-raising method of the present invention, it is characterised in that: in the step g after emerging, throw off surface layer
Withered grass and shading net take shading net on seedling bed, keep seedling bed wet, give within every 2-3 days seedling weeding, while every 5-7
It applies a farmyard manure strong sprout.
The invention has the benefit that the present invention first carries out soaking fermentation to rhizoma polygonati seed, then carry out on culture medium again
Refrigeration, finally breeding in the soil are substantially shorter the hibernation period of rhizoma polygonati seed, and then shorten breeding process, reproductive process letter
It is single, a large amount of rhizoma polygonati seedlings can be quickly provided, reducing the breeding of rhizoma polygonati stem tuber reduces planting cost, promotes rhizoma polygonati artificial growth, subtracts
The excavation of few wild rhizoma polygonati, improves the income of farmer, and by using mating system of the invention, finally obtained rhizoma polygonati
The germination percentage of seed is also higher, is conducive to promote, while by rhizoma polygonati the storage of seeds, convenient for keeping the higher water content of rhizoma polygonati seed,
The loss of nutritional ingredient in rhizoma polygonati seed is avoided, rhizoma polygonati seed increases in dormant stage ABA content, soaked before sending out tooth
Bubble, ABA content can be promoted to reduce, break the suspend mode of rhizoma polygonati seed and bud, while realize rhizoma polygonati wild resource protection and can
Sustainable development utilizes.
Specific embodiment
In order to realize invention technological means, reach purpose and effect is easy to understand, below with reference to specific implementation
Mode, the present invention is further explained.
A kind of embodiment 1: rhizoma polygonati seed seedling-raising method of the present invention, comprising the following steps:
A. it chooses:
In the rhizoma polygonati seed that or so 10-11 month harvesting is mature, wherein picking out the rhizoma polygonati seed of full grains, fruit is gone in stranding
Skin；
B. it impregnates:
Rhizoma polygonati seed in step a is placed in soak after immersion treatment 2-3min, is taken out, rinses two with clear water
Time, wherein soak is made of following material: citric acid 15-19 parts by weight, 1,2-diaminocyclohexane tetraacetic acid 10-13 parts by weight, water
100-120 parts by weight；
C. fine sand is handled:
By the ABT of 5-10mg/L take root amidin, the indolebutyric acid aqueous solution of 10-20mg/L, 100-200mg/L it is more
Bacterium Lingshui Spring solution is uniformly mixed in equal volume, is mixed fine sand with gained mixed liquor wet, and humid control is in 30-50%；
D. it stores:
Shady place storage is placed on after fine sand mixes with 1:3 by treated in rhizoma polygonati seed and step c, temperature maintains 1-
7℃；
E. vernalization:
The seed that the storage processing time is 90-100 days is layered on culture medium, then covers one layer of culture medium above,
Vernalization under 23-27 DEG C of indoor conditions；
F. it sows:
It selects place as nursery, the seed drilling through step e vernalization on ready seedling bed, sows rear cover
Upper withered grass is simultaneously watered, and finally covers shading net again；
G. seedling period management:
After emerging, withered grass and shading net are thrown off, shading net is taken on seedling bed, keeps seedling bed wet, gives seedling
Timely weeding and apply farmyard manure strong sprout.
A kind of embodiment 2: rhizoma polygonati seed seedling-raising method of the present invention, comprising the following steps:
A. it chooses:
In the rhizoma polygonati seed that or so 10-11 month harvesting is mature, wherein picking out the rhizoma polygonati seed of full grains, fruit is gone in stranding
Skin；
B. it impregnates:
Rhizoma polygonati seed in step a is placed in soak after immersion treatment 2-3min, is taken out, rinses two with clear water
Time, wherein soak is made of following material: citric acid 15-19 parts by weight, 1,2-diaminocyclohexane tetraacetic acid 10-13 parts by weight, water
100-120 parts by weight；
C. fine sand is handled:
By the ABT of 5-10mg/L take root amidin, 10-20mg/L indolebutyric acid aqueous solution,
The carbendazim aqueous solution of 100-200mg/L is uniformly mixed in equal volume, and fine sand is mixed to wet, humidity control with gained mixed liquor
System is in 30-50%；
D. it stores:
Shady place storage is placed on after fine sand mixes with 1:3 by treated in rhizoma polygonati seed and step c, temperature maintains 1-
7℃；
E. vernalization:
The seed that the storage processing time is 90-100 days is layered on culture medium, then covers one layer of culture medium above,
Vernalization under 23-27 DEG C of indoor conditions；
F. it sows:
It selects place as nursery, the seed drilling through step e vernalization on ready seedling bed, sows rear cover
Upper withered grass is simultaneously watered, and finally covers shading net again；
G. seedling period management:
After emerging, withered grass and shading net are thrown off, shading net is taken on seedling bed, keeps seedling bed wet, gives seedling
Timely weeding and apply farmyard manure strong sprout.
Mature rhizoma polygonati seed is being harvested 10-11 month in the step a, wherein picking out the rhizoma polygonati kind of full grains
Pericarp is removed in son, stranding, is cleaned with clear water, seed is placed in 30-40 DEG C of strong tea and is impregnated for 24 hours, taking-up is dried.
First soak is made before being impregnated in the step b, 15 parts by weight citric acids and part water are poured into stirred tank
Middle stirring 5min, then 10 parts by weight 1,2-diaminocyclohexane tetraacetic acids and remaining water are poured into stirred tank, continue to stir 5min, it is rear to take out
, then the rhizoma polygonati seed in step a is placed in soak after immersion treatment 2-3min, takes out, is rinsed with water twice.
The take root indolebutyric acid aqueous solution of amidin and 10mg/L of the ABT of 5mg/L is first poured into stirring in the step c
5min is stirred in kettle, then the carbendazim aqueous solution of 100mg/L is poured into stirred tank, continues to stir 5min, it is rear to take out, most
The mixed liquor obtained by is mixed fine sand wet afterwards, and humid control is in 30-50%.
It is per cubic centimeter to mix in wet fine sand by rhizoma polygonati seed fine sand of admixing in step c that treated in the step d
4-8 rhizoma polygonati seeds are admixed, is subsequently placed in and stores shady and coolly, are periodically watered, humidity 30-50% is kept, are stored 3-4 months.
Culture medium in the step e is rice chaff, decomposed leaves or other loose nutrient matrixes through everfermentation
One or more of mixing.
Rhizoma polygonati seed and fine sand after storing step d in the step e are uniformly sowed on culture medium together, are sowed
With a thickness of 0.5-1cm, one layer of culture medium is then sowed again above, form the high ridge of width 80-100cm, high 7-10cm, ridge spacing
For 15-25cm, while also to water sooner or later daily and keep culture medium humidity 30-50%.
The seed through step 5 vernalization is uniformly broadcast by line-spacing 15cm trench digging depth 3-5cm on seedling bed in the step f
Enter in ditch, thickness of earth covering 2.5-3cm is slightly jammed on, and keeps ground moistening, and after planting burning is primary permeable, then inserts arch item, and button is moulded
Expect agricultural film, reinforce arched shed seedbed management, in time ventilation, hardening, when waiting heights of seedling 3cm, spacious night in daytime is covered, and gradually removes arched shed.
After planting the soil moisture is not less than 15 DEG C to the step f, pours soil a water every other week, irrigation amount is to be impregnated with
Subject within topsoil 2cm, the environment of seedling raising process is normal atmospheric environment, and to apply with time-histories every two weeks once has
Machine fertilizer, each dose are no less than 4kg/ ㎡.
In the step g after emerging, withered grass and the shading net on surface layer are thrown off, shading net is taken on seedling bed, kept
Seedling bed is wet, gives seedling weeding, while applying within every 5-7 days a farmyard manure strong sprout within every 2-3 days.
A kind of embodiment 3: rhizoma polygonati seed seedling-raising method of the present invention, comprising the following steps:
Step 1: it chooses
In the rhizoma polygonati seed that harvesting in or so October is mature, wherein picking out the rhizoma polygonati seed of full grains, pericarp is removed in stranding,
It is cleaned with clear water, seed is placed in 30 DEG C of strong teas and is impregnated for 24 hours, taking-up is dried；
Step 2: it impregnates
First soak is made, 15 parts by weight citric acids and part water are poured into and stir 5min in stirred tank, then by 10
Parts by weight 1,2-diaminocyclohexane tetraacetic acid and remaining water pour into stirred tank, continue to stir 5min, rear to take out, then by step 1
In rhizoma polygonati seed be placed in soak after immersion treatment 2min, take out, be rinsed with water twice；
Step 3: fine sand processing
First the take root indolebutyric acid aqueous solution of amidin and 10mg/L of the ABT of 5mg/L is poured into stirred tank and stirred
5min, then the carbendazim aqueous solution of 100mg/L is poured into stirred tank, continue to stir 5min, it is rear to take out, finally use gained
Mixed liquor mixes fine sand wet, and humid control is 40%；
Step 4: storage
By in rhizoma polygonati seed fine sand of admixing in step 3 that treated, per cubic centimeter mix admixes 4 Huangs in wet fine sand
Smart seed, is subsequently placed in and stores shady and coolly, periodically waters, and keeps humidity 30%, stores 3 months, temperature maintains 2 DEG C；
Step 5: vernalization
Rhizoma polygonati seed and fine sand after step 4 is stored uniformly are sowed on culture medium together, sow with a thickness of
Then 0.5cm sows one layer of culture medium again above, form the high ridge of width 80cm, high 7cm, ridge spacing is 15cm, while also being wanted
Sooner or later watering daily keeps culture medium humidity 30%, the vernalization under 25 DEG C of indoor conditions, culture medium be rice chaff through everfermentation,
The mixing of decomposed leaves；
Step 6: sowing
Select place as nursery, the seed drilling through step 5 vernalization on ready seedling bed, in nursery
By line-spacing 15cm trench digging depth 3cm on bed, the seed through step 5 vernalization is uniformly sown into ditch, thickness of earth covering 2.5cm is slightly stepped on
Pressure keeps ground moistening, and after planting burning is primary permeable, then inserts arch item, and fastener agricultural film reinforces arched shed seedbed management, in time
Ventilation, hardening, when waiting heights of seedling 3cm, spacious night in daytime is covered, and gradually removes arched shed, after planting covers withered grass and water, finally covers screening again
Screened negative, while after planting the soil moisture is not less than 15 DEG C, pours soil a water every other week, irrigation amount is to be impregnated with topsoil
Subject within 2cm, the environment of seedling raising process is normal atmospheric environment, to apply an organic fertilizer every two weeks with time-histories, often
Secondary dose is no less than 4kg/ ㎡；
Step 7: seedling period management
After emerging, withered grass and the shading net on surface layer are thrown off, shading net is taken on seedling bed, keeps seedling bed wet,
Give seedling weeding within every 3 days, while every 7 days apply a farmyard manure strong sprout.
A kind of embodiment 4: rhizoma polygonati seed seedling-raising method of the present invention, comprising the following steps:
Step 1: it chooses
In the rhizoma polygonati seed that harvesting in or so October is mature, wherein picking out the rhizoma polygonati seed of full grains, pericarp is removed in stranding,
It is cleaned with clear water, seed is placed in 30 DEG C of strong teas and is impregnated for 24 hours, taking-up is dried；
Step 2: it impregnates
First soak is made, 17 parts by weight citric acids and part water are poured into and stir 5min in stirred tank, then will
11.5 parts by weight 1,2-diaminocyclohexane tetraacetic acids and remaining water pour into stirred tank, continue to stir 5min, rear to take out, then will step
Rhizoma polygonati seed in rapid one is placed in soak after immersion treatment 2min, is taken out, is rinsed with water twice；
Step 3: fine sand processing
First the take root indolebutyric acid aqueous solution of amidin and 10mg/L of the ABT of 5mg/L is poured into stirred tank and stirred
5min, then the carbendazim aqueous solution of 100mg/L is poured into stirred tank, continue to stir 5min, it is rear to take out, finally use gained
Mixed liquor mixes fine sand wet, and humid control is 40%；
Step 4: storage
By in rhizoma polygonati seed fine sand of admixing in step 3 that treated, per cubic centimeter mix admixes 4 Huangs in wet fine sand
Smart seed, is subsequently placed in and stores shady and coolly, periodically waters, and keeps humidity 30%, stores 3 months, temperature maintains 2 DEG C；
Step 5: vernalization
Rhizoma polygonati seed and fine sand after step 4 is stored uniformly are sowed on culture medium together, sow with a thickness of
Then 0.5cm sows one layer of culture medium again above, form the high ridge of width 80cm, high 7cm, ridge spacing is 15cm, while also being wanted
Sooner or later watering daily keeps culture medium humidity 30%, the vernalization under 25 DEG C of indoor conditions, culture medium be rice chaff through everfermentation,
The mixing of decomposed leaves；
Step 6: sowing
Select place as nursery, the seed drilling through step 5 vernalization on ready seedling bed, in nursery
By line-spacing 15cm trench digging depth 3cm on bed, the seed through step 5 vernalization is uniformly sown into ditch, thickness of earth covering 2.5cm is slightly stepped on
Pressure keeps ground moistening, and after planting burning is primary permeable, then inserts arch item, and fastener agricultural film reinforces arched shed seedbed management, in time
Ventilation, hardening, when waiting heights of seedling 3cm, spacious night in daytime is covered, and gradually removes arched shed, after planting covers withered grass and water, finally covers screening again
Screened negative, while after planting the soil moisture is not less than 15 DEG C, pours soil a water every other week, irrigation amount is to be impregnated with topsoil
Subject within 2cm, the environment of seedling raising process is normal atmospheric environment, to apply an organic fertilizer every two weeks with time-histories, often
Secondary dose is no less than 4kg/ ㎡；
Step 7: seedling period management
After emerging, withered grass and the shading net on surface layer are thrown off, shading net is taken on seedling bed, keeps seedling bed wet,
Give seedling weeding within every 3 days, while every 7 days apply a farmyard manure strong sprout.
A kind of embodiment 5: rhizoma polygonati seed seedling-raising method of the present invention, comprising the following steps:
Step 1: it chooses
In the rhizoma polygonati seed that harvesting in or so October is mature, wherein picking out the rhizoma polygonati seed of full grains, pericarp is removed in stranding,
It is cleaned with clear water, seed is placed in 30 DEG C of strong teas and is impregnated for 24 hours, taking-up is dried；
Step 2: it impregnates
First soak is made, 19 parts by weight citric acids and part water are poured into and stir 5min in stirred tank, then by 13
Parts by weight 1,2-diaminocyclohexane tetraacetic acid and remaining water pour into stirred tank, continue to stir 5min, rear to take out, then by step 1
In rhizoma polygonati seed be placed in soak after immersion treatment 2min, take out, be rinsed with water twice；
Step 3: fine sand processing
First the take root indolebutyric acid aqueous solution of amidin and 10mg/L of the ABT of 5mg/L is poured into stirred tank and stirred
5min, then the carbendazim aqueous solution of 100mg/L is poured into stirred tank, continue to stir 5min, it is rear to take out, finally use gained
Mixed liquor mixes fine sand wet, and humid control is 40%；
Step 4: storage
By in rhizoma polygonati seed fine sand of admixing in step 3 that treated, per cubic centimeter mix admixes 4 Huangs in wet fine sand
Smart seed, is subsequently placed in and stores shady and coolly, periodically waters, and keeps humidity 30%, stores 3 months, temperature maintains 2 DEG C；
Step 5: vernalization
Rhizoma polygonati seed and fine sand after step 4 is stored uniformly are sowed on culture medium together, sow with a thickness of
Then 0.5cm sows one layer of culture medium again above, form the high ridge of width 80cm, high 7cm, ridge spacing is 15cm, while also being wanted
Sooner or later watering daily keeps culture medium humidity 30%, the vernalization under 25 DEG C of indoor conditions, culture medium be rice chaff through everfermentation,
The mixing of decomposed leaves；
Step 6: sowing
Select place as nursery, the seed drilling through step 5 vernalization on ready seedling bed, in nursery
By line-spacing 15cm trench digging depth 3cm on bed, the seed through step 5 vernalization is uniformly sown into ditch, thickness of earth covering 2.5cm is slightly stepped on
Pressure keeps ground moistening, and after planting burning is primary permeable, then inserts arch item, and fastener agricultural film reinforces arched shed seedbed management, in time
Ventilation, hardening, when waiting heights of seedling 3cm, spacious night in daytime is covered, and gradually removes arched shed, after planting covers withered grass and water, finally covers screening again
Screened negative, while after planting the soil moisture is not less than 15 DEG C, pours soil a water every other week, irrigation amount is to be impregnated with topsoil
Subject within 2cm, the environment of seedling raising process is normal atmospheric environment, to apply an organic fertilizer every two weeks with time-histories, often
Secondary dose is no less than 4kg/ ㎡；
Step 7: seedling period management
After emerging, withered grass and the shading net on surface layer are thrown off, shading net is taken on seedling bed, keeps seedling bed wet,
Give seedling weeding within every 3 days, while every 7 days apply a farmyard manure strong sprout.
Detection, which is carried out, according to embodiment 3, embodiment 4 and 5 rhizoma polygonati seed seedling-raising method of embodiment show that the present invention is first to Huang
Smart seed carries out soaking fermentation, then carries out refrigerating on culture medium again, finally breeding in the soil, is substantially shorter rhizoma polygonati seed
Hibernation period, make and then shorten breeding process, reproductive process is simple, can quickly provide a large amount of rhizoma polygonati seedlings, reduces rhizoma polygonati block
Stem breeding reduces planting cost, promotes rhizoma polygonati artificial growth, reduces the excavation of wild rhizoma polygonati, improve the income of farmer, and
By using mating system of the invention, the germination percentage of finally obtained rhizoma polygonati seed is also higher.
The present invention first carries out soaking fermentation to rhizoma polygonati seed, then carries out refrigerating on culture medium again, finally educate in the soil
Kind, it is substantially shorter the hibernation period of rhizoma polygonati seed, makes and then shorten breeding process, reproductive process is simple, can quickly provide big
Rhizoma polygonati seedling is measured, reducing the breeding of rhizoma polygonati stem tuber reduces planting cost, and promote rhizoma polygonati artificial growth, reduces the excavation of wild rhizoma polygonati,
The income of farmer is improved, and by using mating system of the invention,
The germination percentage of finally obtained rhizoma polygonati seed is also higher, is conducive to promote, while by rhizoma polygonati the storage of seeds, convenient for keeping
The higher water content of rhizoma polygonati seed, avoids the loss of nutritional ingredient in rhizoma polygonati seed, and rhizoma polygonati seed contains in dormant stage abscisic acid
Amount increases, and is impregnated before sending out tooth, ABA content can be promoted to reduce, broken the suspend mode of rhizoma polygonati seed and bud, realize simultaneously
The protection and sustainable development of rhizoma polygonati wild resource utilize.
The basic principles, main features and advantages of the invention have been shown and described above.The technical staff of the industry should
Understand, the present invention is not limited to the above embodiments, and the above embodiments and description only describe originals of the invention
Reason, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes and improvements
It all fall within the protetion scope of the claimed invention.The claimed scope of the invention is by appended claims and its equivalent circle
It is fixed.
Claims (10)
1. a kind of rhizoma polygonati seed seedling-raising method, it is characterised in that the following steps are included:
A. it chooses:
In the rhizoma polygonati seed that or so 10-11 month harvesting is mature, wherein picking out the rhizoma polygonati seed of full grains, pericarp is removed in stranding；
B. it impregnates:
Rhizoma polygonati seed in step a is placed in soak after immersion treatment 2-3min, is taken out, is rinsed twice with clear water,
Middle soak is made of following material: citric acid 15-19 parts by weight, 1,2-diaminocyclohexane tetraacetic acid 10-13 parts by weight, water 100-120
Parts by weight；
C. fine sand is handled:
The ABT of 5-10mg/L is taken root the carbendazim of amidin, the indolebutyric acid aqueous solution of 10-20mg/L, 100-200mg/L
Aqueous solution is uniformly mixed in equal volume, is mixed fine sand with gained mixed liquor wet, and humid control is in 30-50%；
D. it stores:
Shady place storage is placed on after fine sand mixes with 1:3 by treated in rhizoma polygonati seed and step c, temperature maintains 1-7 DEG C；
E. vernalization:
The seed that the storage processing time is 90-100 days is layered on culture medium, then covers one layer of culture medium above, in 23-27
DEG C indoor conditions under vernalization；
F. it sows:
Place is selected after planting to cover withered the seed drilling through step e vernalization on ready seedling bed as nursery
Grass simultaneously waters, and finally covers shading net again；
G. seedling period management:
After emerging, withered grass and shading net are thrown off, shading net is taken on seedling bed, keeps seedling bed wet, it is timely to seedling
Weeding and apply farmyard manure strong sprout.
2. a kind of rhizoma polygonati seed seedling-raising method according to claim 1, it is characterised in that: in the 10-11 month in the step a
The mature rhizoma polygonati seed of part harvesting, wherein picking out the rhizoma polygonati seed of full grains, pericarp is removed in stranding, is cleaned with clear water, by seed
It is placed in 30-40 DEG C of strong tea and impregnates for 24 hours, taking-up is dried.
3. a kind of rhizoma polygonati seed seedling-raising method according to claim 1, it is characterised in that: before being impregnated in the step b first
Soak is made, 15 parts by weight citric acids and part water are poured into and stir 5min in stirred tank, then by 10 parts by weight hexamethylenes
Ethylenediamine tetraacetic acid (EDTA) and remaining water pour into stirred tank, continue to stir 5min, rear to take out, then by the rhizoma polygonati kind in step a
Son is placed in soak after immersion treatment 2-3min, is taken out, is rinsed with water twice.
4. a kind of rhizoma polygonati seed seedling-raising method according to claim 1, it is characterised in that: first by 5mg/L in the step c
The take root indolebutyric acid aqueous solution of amidin and 10mg/L of ABT pour into and stir 5min in stirred tank, then by the more of 100mg/L
Bacterium Lingshui Spring solution pours into stirred tank, continues to stir 5min, rear to take out, finally the mixed liquor obtained by is mixed fine sand wet, wet
Degree control is in 30-50%.
5. a kind of rhizoma polygonati seed seedling-raising method according to claim 1, it is characterised in that: by rhizoma polygonati kind in the step d
In son admixes in step c that treated fine sand, per cubic centimeter mix admixes 4-8 rhizoma polygonati seeds in wet fine sand, be subsequently placed in
It stores, periodically waters shady and coolly, keep humidity 30-50%, store 3-4 months.
6. a kind of rhizoma polygonati seed seedling-raising method according to claim 1, it is characterised in that: the culture medium in the step e
It is mixed for one or more of rice chaff, decomposed leaves or other loose nutrient matrixes through everfermentation.
7. a kind of rhizoma polygonati seed seedling-raising method according to claim 1, it is characterised in that: store step d in the step e
Rhizoma polygonati seed and fine sand behind hiding are uniformly sowed on culture medium together, are sowed with a thickness of 0.5-1cm, are then broadcast again above
One layer of culture medium is spread, forms the high ridge of width 80-100cm, high 7-10cm, ridge spacing is 15-25cm, while also to be poured sooner or later daily
Water keeps culture medium humidity 30-50%.
8. a kind of rhizoma polygonati seed seedling-raising method according to claim 1, it is characterised in that: in seedling bed in the step f
It is upper to press line-spacing 15cm trench digging depth 3-5cm, the seed through step 5 vernalization is uniformly sown into ditch, thickness of earth covering 2.5-3cm, slightly
It jams on, keeps ground moistening, after planting burning is primary permeable, then inserts arch item, and fastener agricultural film reinforces arched shed seedbed management, and
Shi Tongfeng, hardening, when waiting heights of seedling 3cm, spacious night in daytime is covered, and gradually removes arched shed.
9. a kind of rhizoma polygonati seed seedling-raising method according to claim 1, it is characterised in that: the step f after planting soil
Temperature is not less than 15 DEG C, pours soil a water every other week, irrigation amount, which is subject to, to be impregnated within topsoil 2cm, nursery
The environment of journey is normal atmospheric environment, to apply an organic fertilizer every two weeks with time-histories, each dose is no less than 4kg/
㎡。
10. a kind of rhizoma polygonati seed seedling-raising method according to claim 1, it is characterised in that: wait emerge in the step g
Afterwards, withered grass and the shading net for throwing off surface layer take shading net on seedling bed, keep seedling bed wet, remove to seedling within every 2-3 days
Grass, while applying within every 5-7 days a farmyard manure strong sprout.
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| CN110537468A (en) * | 2019-09-25 | 2019-12-06 | 贵州百灵企业集团制药股份有限公司 | simple polygonatum cyrtonema seed seedling culture method |
| CN110810198A (en) * | 2019-12-05 | 2020-02-21 | 昆明英武农业科技有限公司 | Germination accelerating substrate for polygonatum kingianum seeds and germination accelerating method thereof |
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