CN103238436A - Aperture disk transplanting provisonal planting method of original sugarcane detoxification seedlings - Google Patents

Aperture disk transplanting provisonal planting method of original sugarcane detoxification seedlings Download PDF

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CN103238436A
CN103238436A CN2013101724148A CN201310172414A CN103238436A CN 103238436 A CN103238436 A CN 103238436A CN 2013101724148 A CN2013101724148 A CN 2013101724148A CN 201310172414 A CN201310172414 A CN 201310172414A CN 103238436 A CN103238436 A CN 103238436A
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seedlings
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武媛丽
杨本鹏
蔡文伟
曾军
张树珍
伍苏然
王俊刚
彭李顺
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Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences
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Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences
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Abstract

本发明涉及一种甘蔗脱毒原种苗穴盘移栽假植方法,包括前期准备过程、移栽假植及管理过程和水肥药管理过程。本发明采用露天穴盘移栽假植的方式,解决了甘蔗脱毒原种苗温室大棚裸根移栽成本高、运输难、种植成活率低的问题,解决了原种苗露天裸根移栽出圃时根系易损、缓苗期长的问题,有效地提高原种苗育苗和出圃种植的成活率,对甘蔗脱毒种苗的标准化、规模化、产业化育苗提供了技术支撑,对甘蔗脱毒种苗及配套栽培技术的推广应用起到了积极的推动作用。The invention relates to a method for transplanting and artificially planting sugarcane detoxified original seedlings in socket trays, which includes a pre-preparation process, a transplanting and artificial planting and management process, and a water, fertilizer and medicine management process. The invention adopts the method of open-air hole tray transplanting and artificial planting, solves the problems of high cost, difficult transportation, and low planting survival rate of bare-root transplanting of sugarcane detoxified original seedlings in greenhouses, and solves the problems of open-air bare-root transplantation of original seedlings The root system is vulnerable and the slow seedling period is long when it comes out of the nursery. It can effectively improve the survival rate of the original seedlings and planting out of the nursery. It provides technical support for the standardization, scale and industrialization of sugarcane seedlings. The promotion and application of poisonous seedlings and supporting cultivation techniques have played a positive role in promoting.

Description

一种甘蔗脱毒原种苗穴盘移栽假植方法A method for transplanting and false planting of sugarcane detoxified original seedlings

技术领域 technical field

本发明属植物栽培领域,涉及一种甘蔗种苗的移栽方法,特别是一种甘蔗脱毒原种苗穴盘移栽假植方法。The invention belongs to the field of plant cultivation, and relates to a method for transplanting sugarcane seedlings, in particular to a method for transplanting sugarcane detoxified original seedlings into sockets.

技术背景 technical background

甘蔗(Saccharum officinarum L.)是最重要的糖料作物,也是轻工、化工及能源的重要原材料之一。中国作为世界主要产糖国之一,甘蔗食糖在我国食糖的总量占到94%左右。因此,发展甘蔗生产,对提高人民的生活质量、促进农业及相关产业的发展,乃至对整个国民经济的发展都具有重要的地位和作用。Sugarcane (Saccharum officinarum L.) is the most important sugar crop and one of the important raw materials for light industry, chemical industry and energy. China is one of the major sugar producing countries in the world, and sugarcane sugar accounts for about 94% of the total sugar in my country. Therefore, the development of sugarcane production plays an important role in improving the quality of life of the people, promoting the development of agriculture and related industries, and even the development of the entire national economy.

我国甘蔗主要分布在北纬24°以南的热带及亚热带地区,以广西、云南、广东和海南等省区为主要产区。“十二五”规划指出,糖料蔗的栽培面积要稳定在2900万亩,总产量达1.4亿吨以上,以保障国内食糖消费基本自给。my country's sugarcane is mainly distributed in tropical and subtropical regions south of 24° north latitude, with Guangxi, Yunnan, Guangdong and Hainan provinces as the main production areas. The "Twelfth Five-Year Plan" pointed out that the cultivation area of sugar cane should be stabilized at 29 million mu, and the total output should reach more than 140 million tons, so as to ensure the basic self-sufficiency of domestic sugar consumption.

在甘蔗生产中,一般多采用无性繁殖,经多年种植后,容易受到多种病原物侵染,使种性退化,造成甘蔗含糖量和产量下降。甘蔗病原物侵染以花叶病和宿根矮化病的影响最为严重,且普通的物理或化学方法难以根除,成为制约我国甘蔗单产及含糖量提高的主要因素。In the production of sugarcane, asexual reproduction is generally used. After years of planting, it is easily infected by various pathogens, which degrades the species, resulting in a decrease in sugar content and yield of sugarcane. Mosaic disease and perennial root dwarf disease are the most serious pathogens infecting sugarcane, and common physical or chemical methods are difficult to eradicate, which has become the main factor restricting the increase of sugarcane yield and sugar content in my country.

我国科研单位提出的采用热处理结合腋芽分生组织培养技术,从甘蔗茎段综合脱毒、无突变快繁、病原快速检测、田间扩繁与配套栽培技术等5个方面进行研究,试验表明通过以上步骤获得的甘蔗脱毒健康种苗不含病原菌,可使甘蔗单产提高30%以上,含糖量提高1个百分点以上,节约用种量60%,成功解决了我国甘蔗种性退化及良种繁育上的技术难题。my country's scientific research institutes proposed the use of heat treatment combined with axillary bud meristem culture technology to conduct research on five aspects including comprehensive detoxification of sugarcane stems, rapid propagation without mutations, rapid detection of pathogens, field propagation and supporting cultivation techniques, and the tests showed that the above The detoxified healthy sugarcane seedlings obtained in the steps do not contain pathogenic bacteria, can increase the yield of sugarcane by more than 30%, increase the sugar content by more than 1 percentage point, save 60% of the amount of seeds used, and successfully solve the problems of sugarcane species degradation and breeding of improved varieties in my country technical problems.

目前常规的甘蔗脱毒原种苗移栽方式多数是在温室大棚进行,在棚里利用塑料袋(钵)育苗,也有在棚里进行裸根育苗,其优点是原种苗移栽成活率高,但生产成本高和运输难度大,耗时费工。也有采用露天裸根移栽育苗,育苗成本低,但移栽出圃根系易损、田间种植时成活率低、幼苗缓苗期长,不利于我国甘蔗产业的发展。At present, most of the conventional ways of transplanting the original seedlings of sugarcane virus-free are carried out in the greenhouse, and the seedlings are raised in plastic bags (bowls) in the shed, and there are also bare-root seedlings in the shed. The advantage is that the original seedlings have a high survival rate. , but the production cost is high and the transportation is difficult, time-consuming and labor-intensive. There are also open-air bare-root transplanting seedlings, which are low in seedling cost, but the root system is vulnerable when transplanted out of the nursery, the survival rate is low when planting in the field, and the seedling slowing period is long, which is not conducive to the development of my country's sugarcane industry.

发明内容 Contents of the invention

本发明的目的是针对现有技术的不足而提供一种甘蔗脱毒原种苗穴盘移栽假植方法,采用露天穴盘移栽的方式,解决了甘蔗脱毒种苗原种苗的移栽假植问题,可以进行规模化育苗,运输简便,成本低,田间种植成活率高、种苗恢复生长快,是集炼苗、移栽驯化、假植为一体的新型移栽方法。The purpose of the present invention is to provide a method for transplanting the original seedlings of sugarcane detoxified seedlings in plug trays for the deficiencies in the prior art. For the problem of planting and artificial planting, large-scale seedling cultivation can be carried out, the transportation is simple, the cost is low, the survival rate of field planting is high, and the recovery and growth of seedlings are fast. It is a new transplanting method that integrates seedling hardening, transplanting and domestication, and artificial planting.

本发明所采用的技术方案:The technical scheme adopted in the present invention:

一种甘蔗脱毒原种苗穴盘移栽假植方法,包括前期准备过程、移栽假植及管理过程和水肥药管理过程,其步骤如下:A method for transplanting and artificially transplanting sugarcane detoxified original seedlings into socket trays, comprising a pre-preparation process, a transplanting and artificial planting and management process, and a water and fertilizer management process, the steps of which are as follows:

1、前期准备过程1. Preparatory process

包括苗床准备工序、基质配制工序、穴盘基质填装与杀菌工序。It includes seedbed preparation process, matrix preparation process, tray matrix filling and sterilization process.

苗床准备工序:将所选取的苗圃地进行旋耕、深犁、旋耕、起畦;在苗床上撒施杀虫剂,并将杀虫剂与畦面细土拌匀、耙平,用萌前除草剂喷洒。Seedbed preparation process: perform rotary tillage, deep plowing, rotary tillage, and furrowing on the selected nursery; Before herbicide spraying.

所述苗床的规格为宽1米,长10米。The specifications of the seedbed are 1 meter wide and 10 meters long.

基质配制工序:将细土、保湿材料、有机肥、无机肥和杀虫剂充分拌匀而制得,其中按重量比计各组分的配比为:细土70~80、保湿材料8~12、有机肥8~12、无机肥4~6、杀虫剂0.1~0.3。所述保湿材料是指含水量为50~60%的椰糠、腐熟的蔗渣或木糠。所述有机肥是指腐熟的人或动物(猪、牛、羊、鸡等蓄禽粪便中的1种)的粪便、饼肥。所述无机肥是指尿素、钾肥和过磷酸钙的混合物,其中按重量比计尿素:钾肥:过磷酸钙=6~7:8~10:3~4。Substrate preparation process: fully mix the fine soil, moisturizing material, organic fertilizer, inorganic fertilizer and insecticide, and the ratio of each component by weight is: fine soil 70-80, moisturizing material 8-80 12. 8-12 organic fertilizers, 4-6 inorganic fertilizers, and 0.1-0.3 pesticides. The moisturizing material refers to coconut bran, decomposed bagasse or wood bran with a water content of 50-60%. The organic fertilizer refers to the feces and cake fertilizers of decomposed human or animals (one of poultry manures such as pigs, cattle, sheep, chickens, etc.). The inorganic fertilizer refers to a mixture of urea, potassium fertilizer and superphosphate, wherein urea:potassium fertilizer:superphosphate=6-7:8-10:3-4 by weight.

本发明中,基质的各组分重量比优选:细土70、保湿材料10、有机肥7、无机肥3、杀虫剂0.2。In the present invention, the weight ratio of each component of the substrate is preferably: fine soil 70, moisturizing material 10, organic fertilizer 7, inorganic fertilizer 3, and insecticide 0.2.

穴盘基质填装与杀菌工序:取穴盘,将穴盘按照苗床规格平放于苗床上;将配制好的基质填装满穴孔,再在表面覆盖1.5~2.5㎝厚的保湿材料,淋透水;在移栽前2~3天,用杀菌剂淋洒穴盘基质,然后用塑料薄膜将穴盘盖严,移苗前1天打开塑料薄膜,再浇水淋透。Plug matrix filling and sterilization process: take the plug tray, place the tray flat on the seedbed according to the seedbed specifications; fill the holes with the prepared matrix, and then cover the surface with a 1.5-2.5cm thick moisturizing material, pour Water-permeable; 2-3 days before transplanting, spray the plug matrix with fungicide, then cover the plug tightly with plastic film, open the plastic film 1 day before transplanting, and then water it thoroughly.

所述穴盘规格为28×54㎝,穴孔规格为4~5×8~9㎝或6~7×8~9㎝。The specification of the hole plate is 28×54cm, and the specification of the holes is 4-5×8-9cm or 6-7×8-9cm.

2、移栽假植及管理过程2. Transplanting and artificial planting and management process

将种植于组培装置的脱毒原种苗放置在阴凉通风处2~3天,然后打开组培装置再放置1~2天进行炼苗;将经过炼苗后的脱毒原种苗取出,剪去幼苗2~3㎝的叶片,将培养基清洗干净,再用杀菌剂800倍液浸泡3~5分钟,取出在阴凉处晾干水分;取填装基质的穴盘,扒开穴孔上层的保湿材料后将已清洗处理过的脱毒原种苗栽入穴孔的基质中,深1.5~2厘米,每穴1株,淋透定根水;在苗床上方设置遮阳网,温度低于25℃时,加盖一层塑料薄膜, 3~5天淋1次水,13~15天撤出塑料膜,15~20天拆撤出遮阳网;脱毒原种苗在穴盘里生长45~75天(根据移栽时气候,冬季育苗时间长,夏季育苗时间短),种苗主茎开始出现分蘖,苗长至20~30㎝高,即可出圃。Place the detoxified original seedlings planted in the tissue culture device in a cool and ventilated place for 2 to 3 days, then open the tissue culture device and place them for 1 to 2 days for hardening; take out the detoxified original seedlings after hardening, Cut off the 2-3 cm leaves of the seedlings, clean the culture medium, soak in 800 times of fungicide for 3-5 minutes, take out and dry the water in a cool place; take the tray filled with the substrate, and peel off the upper layer of the hole Plant the cleaned and treated detoxified original seedlings into the matrix of the holes, with a depth of 1.5 to 2 cm, one plant per hole, and drenched with fixed root water; set a sunshade net above the seedbed, and the temperature is lower than At 25°C, cover with a layer of plastic film, pour water once in 3 to 5 days, remove the plastic film in 13 to 15 days, remove the sunshade net in 15 to 20 days; ~75 days (according to the climate at the time of transplanting, the seedling time is long in winter and short in summer), the main stem of the seedlings begins to appear tillers, and the seedlings can be out of the nursery when they grow to a height of 20-30 cm.

3、水肥药管理过程3. Water and fertilizer management process

包括水分管理、营养管理、害虫管理三个方面。Including water management, nutrient management and pest management.

水分管理:脱毒原种苗在穴盘移栽后,需要保持基质湿润,撤出塑料膜后基质保持潮湿、原种苗不出现萎蔫。Water management: After the virus-free original seedlings are transplanted in the plug tray, the substrate needs to be kept moist. After the plastic film is removed, the substrate remains moist and the original seedlings do not wilt.

营养管理:在撤出塑料膜后,开始淋施水肥,每4~6天淋施1次,第1~3次用0.3%的尿素水溶液按10kg/m2喷洒;第4次用0.4%的尿素水溶液按10kg/m2喷洒,以后按此浓度及用量进行淋施,出苗前10天不再淋施水肥。Nutrient management: After the plastic film is withdrawn, start to spray water and fertilizer, once every 4 to 6 days, spray with 0.3% urea aqueous solution at 10kg/ m2 for the 1st to 3rd time; use 0.4% urea for the 4th time The urea aqueous solution is sprayed at 10kg/ m2 , and then drenched according to this concentration and dosage, and water and fertilizer are no longer drenched 10 days before emergence.

害虫管理:幼苗在穴盘生长过程中,需要进行防害虫管理,在撤膜10天后需用药剂(杜邦康宽等)喷施(喷药浓度按使用说明执行)1次,出圃前一周再喷施1次的过程。Pest management: During the growth of the seedlings in the plug tray, pest control management is required. After 10 days of removing the film, it needs to be sprayed with chemicals (DuPont Kangkuan, etc.) (the spraying concentration should be carried out according to the instructions for use) once, and then sprayed one week before leaving the nursery The process of applying 1 time.

本发明采用露天穴盘移栽假植的方式,解决了甘蔗脱毒原种苗温室大棚裸根移栽成本高、运输难、种植成活率低的问题或温室大棚营养袋(钵)育苗移栽成本高、运输成本高的问题,也解决了原种苗露天裸根移栽出圃时根系易损、缓苗期长的问题,有效地提高原种苗育苗和出圃种植的成活率,对甘蔗脱毒种苗的标准化、规模化、产业化育苗提供了技术支撑,对甘蔗脱毒种苗及配套栽培技术的推广应用起到了积极的推动作用。The invention adopts the method of open-air hole tray transplanting and artificial planting, which solves the problems of high cost, difficult transportation, and low planting survival rate of bare-root transplanting of sugarcane original seedlings in greenhouses, or the transplanting of seedlings raised in nutritional bags (bowls) in greenhouses. The problem of high cost and high transportation cost also solves the problem of vulnerable root system and long seedling period when the original seedlings are transplanted out of the nursery with bare roots in the open air, effectively improving the survival rate of the original seedlings and planting out of the nursery. The standardized, large-scale and industrialized breeding of poisonous seedlings has provided technical support, and played a positive role in promoting the promotion and application of sugarcane virus-free seedlings and supporting cultivation techniques.

具体实施方式 Detailed ways

下面结合实施例,对本发明的具体实施方式作进一步详细描述。以下实施例用于说明本发明,但不用来限制本发明的范围。下列实施例中未注明具体条件的实验方法,通常按照常规条件,或按照制造厂商所建议的条件。Below in conjunction with the examples, the specific implementation of the present invention will be further described in detail. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. The experimental methods that do not indicate specific conditions in the following examples are generally in accordance with conventional conditions, or in accordance with the conditions suggested by the manufacturer.

实施例一Embodiment one

1、露天苗床准备1. Open-air seedbed preparation

将所选取的苗圃地进行旋耕(15—30厘米)、深犁(35—45厘米)、旋耕(20—30厘米)、起畦,畦高0.10米,苗床面宽1米,长10米,畦沟宽0.5米。在苗床上均匀地撒施5%辛硫磷、3.6%杀虫双各0.2㎏,然后与畦面5㎝左右细土拌匀、耙平,用萌前除草剂喷洒苗床。Carry out rotary tillage (15-30 cm), deep plow (35-45 cm), rotary tillage (20-30 cm) and furrowing on the selected nursery land. meters, furrow width 0.5 meters. Evenly spread 5% phoxim and 0.2kg of 3.6% dimehypo on the seedbed, then mix with fine soil about 5 cm from the furrow surface, rake it flat, and spray the seedbed with pre-emergence herbicide.

2、基质的配制2. Matrix preparation

在苗床旁边按1个苗床(10平方米)约100㎏基质准备。基质配比:细土75㎏,含水50%的椰糠10㎏,有机肥10㎏,无机肥5㎏,农药0.2㎏,充分拌匀。Prepare about 100kg of substrate for 1 seedbed (10 square meters) next to the seedbed. Substrate ratio: 75kg of fine soil, 10kg of coconut peat with 50% water content, 10kg of organic fertilizer, 5kg of inorganic fertilizer, 0.2kg of pesticide, mix well.

3、有机肥配制3. Organic fertilizer preparation

将人和动物(猪、牛、羊、鸡等蓄禽粪便中的1种)粪便、饼肥等,使用前3个月需将所选取的粪便进行堆沤、发酵,使用时按照配制要求进行。配制时,按100㎏基质加堆沤腐熟的有机肥10㎏,充分拌匀。Human and animal manure (one of the poultry manures such as pigs, cows, sheep, chickens, etc.) manure, cake fertilizer, etc., need to be composted and fermented 3 months before use, and used in accordance with the preparation requirements. When preparing, add 10 kg of decomposed organic fertilizer to 100 kg of substrate, and mix well.

4、无机肥配制4. Inorganic fertilizer preparation

是指尿素、钾肥、过磷酸钙的混合物。在配制时,按100㎏基质加含量为46%的尿素1.5㎏、60%的氯化钾1.5㎏、16%的普通过磷酸钙2㎏,并将该无机肥充分混合后与土壤拌匀。Refers to the mixture of urea, potassium fertilizer and superphosphate. When preparing, add 1.5kg of 46% urea, 1.5kg of 60% potassium chloride, and 2kg of 16% ordinary calcium superphosphate to 100kg of substrate, and mix the inorganic fertilizer thoroughly with the soil.

5、农药5. Pesticides

采用触杀性和内吸性两种农药。触杀性的选取5%辛硫磷颗粒剂,内吸性选取3.6%杀虫双颗粒剂。每100㎏基质里添加上述农药各0.1㎏。Both contact and systemic pesticides are used. Select 5% phoxim granules for contact action, and 3.6% insecticidal double granules for systemic action. Add 0.1kg of the above pesticides to every 100kg of matrix.

6、穴盘6. Cave plate

穴盘准备:购置穴盘规格为28×54㎝,穴孔(50孔/张)规格为4—5×8—9㎝的塑料穴盘,并将穴盘平铺于苗床上。每10平方米(即1畦苗床)放置54张穴盘。Hole plate preparation: Purchase a plastic hole plate with a size of 28×54cm and a hole size (50 holes/sheet) of 4-5×8-9cm, and spread the hole plate on the seedbed. Place 54 plugs per 10 square meters (that is, 1 bed).

基质填装:将已配制好的基质填装满穴孔,再在表面覆盖2㎝厚的腐熟的蔗渣作为保湿材料,淋透水。Substrate filling: fill the holes with the prepared substrate, and then cover the surface with 2 cm thick decomposed bagasse as a moisturizing material, and drench with water.

基质杀菌:在移栽前3天,对穴盘基质进行淋洒杀菌剂,每平方米苗床淋50%多菌灵500倍液10公斤,淋洒后用塑料薄膜将穴盘盖严,移苗前1天打开塑料薄膜,再浇水淋透的过程。Substrate sterilization: 3 days before transplanting, spray the fungicide on the substrate of the hole plate, spray 10 kg of 50% carbendazim 500 times solution per square meter of seedbed, cover the hole plate tightly with a plastic film after spraying, and transplant the seedlings. The process of opening the plastic film one day before and then watering it thoroughly.

7、原种苗穴盘移栽假植7. Transplanting the original seedlings into plug trays for artificial planting

原种苗炼苗:从培养室取出1000株甘蔗脱毒原种苗,放置在移苗地附近阴凉通风处(不要淋雨,不要让太阳光照射)3天,然后剪开组培袋的袋口(若是培养瓶,则打开瓶盖),再放置1天。Hardening of original seedlings: Take out 1000 sugarcane detoxified original seedlings from the cultivation room, place them in a cool and ventilated place near the transplanting site (no rain, no sunlight) for 3 days, and then cut the bag of the tissue culture bag (if it is a culture bottle, open the bottle cap), and then leave it for 1 day.

原种苗清洗杀菌:将经过炼苗后的原种苗取出,剪去幼苗2—3㎝的叶片,放在塑料大盆里,用流动的清水将培养基清洗干净,然后用75%百菌清800倍液浸泡根系3分钟,取出在阴凉处晾干水分。Cleaning and sterilization of the original seedlings: Take out the original seedlings after hardening, cut off the 2-3cm leaves of the seedlings, put them in a large plastic basin, clean the medium with flowing water, and then use 75% bacteriostasis Soak the root system in clear 800 times liquid for 3 minutes, take it out and dry the water in a cool place.

原种苗移栽:移栽时用手指轻轻扒开(深约2㎝)穴孔上层的保湿材料(腐熟的蔗渣)后,将已清洗处理过的原种苗栽入穴孔的基质中,深约1.5—2厘米,每穴1株,然后用手指轻轻压实,淋透定根水(原种苗移栽后,需要保持基质湿润)。用2.5—3米长的竹签或铁线折成弧形,插在苗床两边,盖上遮阳网,温度低于25℃时,加盖1层塑料薄膜,3天打开淋1次水,15天撤出塑料膜【撤出塑料膜即开始淋施水肥,每4天淋施1次,第1—3次按0.3%的尿素水溶液(即100㎏水加0.3㎏尿素,充分混匀)淋施,淋施时按10kg/m2喷洒;第4次按0.4%的尿素水(即100㎏水加0.4㎏尿素)按10kg/m2喷洒,以后每隔4天按此浓度及用量淋施1次,出苗前10天不再淋施水肥】,16天拆撤出遮阳网;原种苗生长55天,种苗主茎开始出现分蘖,苗长至20—30㎝高,即可出圃(出圃前3—5天不要淋水,以保持穴孔里的土团紧实)。幼苗在穴盘生长过程中,需要防止害虫的危害,在撤膜10天后需用杜邦康宽等药剂喷施(喷药浓度按使用说明执行)1次,出圃前一周再喷施1次。Transplanting original seedlings: When transplanting, use your fingers to gently remove the moisture-retaining material (decomposed bagasse) on the upper layer of the hole (about 2cm deep), and then plant the cleaned original seedlings into the matrix of the hole , about 1.5-2 cm deep, 1 plant per hole, then gently compact it with your fingers, and drench the rooting water (after transplanting the original seedlings, the substrate needs to be kept moist). Use a 2.5-3 meter long bamboo stick or iron wire to bend into an arc, insert it on both sides of the seedbed, cover with a sunshade net, and cover it with a layer of plastic film when the temperature is lower than 25°C, open it every 3 days and pour water once, 15 Withdraw the plastic film within 1 day [After the plastic film is removed, water and fertilizer will be sprayed, once every 4 days, and the first to third time will be sprayed with 0.3% urea aqueous solution (that is, 100 kg of water plus 0.3 kg of urea, fully mixed) Spray at 10kg/ m2 when spraying; spray at 10kg/ m2 with 0.4% urea water (100kg water plus 0.4kg urea) for the fourth time, and spray at this concentration and dosage every 4 days thereafter 1 time, do not spray water and fertilizer 10 days before emergence], remove the sunshade net 16 days; the original seedlings grow for 55 days, the main stem of the seedlings begins to appear tillers, and the seedlings grow to 20-30 cm high, and then they can be out of the nursery ( Do not pour water 3-5 days before leaving the nursery to keep the soil in the hole tight). During the growth process of the seedlings, it is necessary to prevent the harm of pests. After 10 days of removing the film, it needs to be sprayed with DuPont Kangkuan and other chemicals (the spraying concentration is carried out according to the instructions for use) once, and then sprayed once a week before leaving the nursery.

实施例二Embodiment two

1、露天苗床准备1. Open-air seedbed preparation

同实施例一。Same as embodiment one.

2、基质的配制2. Matrix preparation

在苗床旁边按1个苗床(10平方米)约100㎏基质准备。基质配比:细土72㎏,含水54%的腐熟蔗渣10㎏,有机肥12㎏,无机肥6㎏,农药0.2㎏,充分拌匀。Prepare about 100kg of substrate for 1 seedbed (10 square meters) next to the seedbed. Substrate ratio: fine soil 72kg, decomposed bagasse with 54% water content 10kg, organic fertilizer 12kg, inorganic fertilizer 6kg, pesticide 0.2kg, mix well.

3、有机肥配制3. Organic fertilizer preparation

同实施例一Same as Example 1

4、无机肥配制4. Inorganic fertilizer preparation

是指尿素、钾肥、过磷酸钙的混合物。在配制时,按100㎏基质加含量为46%的尿素1.4㎏、60%的氯化钾1.6㎏、16%的普通过磷酸钙2.3㎏,并将该无机肥充分混合后与土壤拌匀。Refers to the mixture of urea, potassium fertilizer and superphosphate. When preparing, add 1.4kg of 46% urea, 1.6kg of 60% potassium chloride, and 2.3kg of 16% ordinary calcium superphosphate to 100kg of substrate, and mix the inorganic fertilizer thoroughly with the soil.

5、农药5. Pesticides

采用触杀性和内吸性两种农药。触杀性的选取5%辛硫磷颗粒剂,内吸性选取3.6%杀虫双颗粒剂。每100㎏基质里添加上述农药各0.1㎏。Both contact and systemic pesticides are used. Select 5% phoxim granules for contact action, and 3.6% insecticidal double granules for systemic action. Add 0.1kg of the above pesticides to every 100kg of matrix.

6、穴盘6. Cave plate

穴盘准备:购置穴盘规格为28×54㎝,穴孔(32孔/张)规格为6—7×8—9㎝的塑料穴盘,并将穴盘平铺于苗床上。每10平方米(即1畦苗床)放置54张穴盘。Hole plate preparation: purchase a plastic hole plate with a size of 28×54cm and a hole size (32 holes/sheet) of 6-7×8-9cm, and spread the hole plate on the seedbed. Place 54 plugs per 10 square meters (that is, 1 bed).

基质填装:将已配制好的基质填装满穴孔,再在表面覆盖2㎝厚的腐熟的木糠作为保湿材料,淋透水。Substrate filling: Fill the holes with the prepared substrate, and then cover the surface with 2 cm thick decomposed wood bran as a moisturizing material, and drench with water.

基质杀菌:在移栽前2天,对穴盘基质进行淋洒杀菌剂,每平方米苗床淋75%百菌清500倍液10公斤,淋洒后用塑料薄膜将穴盘盖严,移苗前1天打开塑料薄膜,再浇水淋透的过程。Substrate sterilization: 2 days before transplanting, spray the fungicide on the substrate of the hole plate, spray 10 kg of 75% chlorothalonil 500 times solution per square meter of seedbed, cover the hole plate tightly with a plastic film after spraying, and transplant the seedlings. The process of opening the plastic film one day before and then watering it thoroughly.

7、原种苗穴盘移栽假植7. Transplanting the original seedlings into plug trays for artificial planting

原种苗炼苗:从培养室取出1000株甘蔗脱毒原种苗,放置在移苗地附近阴凉通风处(不要淋雨,不要让太阳光照射)2天,然后剪开组培袋的袋口(若是培养瓶,则打开瓶盖),再放置2天。Hardening of original seedlings: Take 1000 sugarcane detoxified original seedlings from the cultivation room, place them in a cool and ventilated place near the transplanting site (no rain, no sunlight) for 2 days, and then cut open the bag of the tissue culture bag (if it is a culture bottle, open the bottle cap), and leave it for another 2 days.

原种苗清洗杀菌:将经过炼苗后的原种苗取出,剪去幼苗2—3㎝的叶片,放在塑料大盆里,用流动的清水将培养基清洗干净,然后用50%多菌灵800倍液浸泡根系4分钟,取出在阴凉处晾干水分。Cleaning and sterilization of the original seedlings: Take out the original seedlings after hardening, cut off the 2-3cm leaves of the seedlings, put them in a large plastic basin, clean the medium with flowing water, and then use 50% multi-bacteria Ling 800 times solution to soak the root system for 4 minutes, take it out and dry the water in a cool place.

原种苗移栽:移栽时用手指轻轻扒开(深约2㎝)穴孔上层的保湿材料(腐熟的木糠)后,将已清洗处理过的原种苗栽入穴孔的基质中,深约1.5—2厘米,每穴1株,然后用手指轻轻压实,淋透定根水(原种苗移栽后,需要保持基质湿润)。用2.5—3米长的竹签或铁线折成弧形,插在苗床两边,盖上遮阳网,温度低于25℃时,加盖1层塑料薄膜,4天打开淋1次水,13天撤出塑料膜【撤出塑料膜即开始淋施水肥,每5天淋施1次,第1—3次按0.3%的尿素水溶液(即100㎏水加0.3㎏尿素,充分混匀)淋施,淋施时按10kg/m2喷洒;第4次按0.4%的尿素水(即100㎏水加0.4㎏尿素)按10kg/m2喷洒,以后每隔5天按此浓度及用量淋施1次,出苗前10天不再淋施水肥】,18天拆撤出遮阳网;原种苗生长65天,种苗主茎开始出现分蘖,苗长至20—30㎝高,即可出圃(出圃前3—5天不要淋水,以保持穴孔里的土团紧实)。幼苗在穴盘生长过程中,需要防止害虫的危害,在撤膜10天后需用杜邦康宽等药剂喷施(喷药浓度按使用说明执行)1次,出圃前一周再喷施1次。Original seedling transplanting: When transplanting, use your fingers to gently remove the moisture-retaining material (decomposed wood bran) on the upper layer of the hole (about 2cm deep), and then plant the cleaned and treated original seedlings into the substrate of the hole Medium, about 1.5-2 cm deep, 1 plant per hole, then gently compact it with your fingers, and drench the rooting water (after transplanting the original seedlings, the substrate needs to be kept moist). Use a 2.5-3 meter long bamboo stick or iron wire to bend into an arc, insert it on both sides of the seedbed, and cover it with a sunshade net. When the temperature is lower than 25°C, cover it with a layer of plastic film, open it every 4 days and pour water once, 13 Withdraw the plastic film every day [withdraw the plastic film and start to spray water and fertilizer, once every 5 days, and spray with 0.3% urea aqueous solution (that is, add 0.3 kg urea to 100 kg water, mix well) for the first to third time Spray at 10kg/ m2 when spraying; spray at 10kg/ m2 with 0.4% urea water (i.e. 100kg water plus 0.4kg urea) for the fourth time, and spray at this concentration and dosage every 5 days thereafter 1 time, do not spray water and fertilizer 10 days before emergence], remove the sunshade net 18 days; the original seedlings grow for 65 days, the main stem of the seedlings begins to appear tillers, and the seedlings grow to 20-30 cm high, and then they can be out of the nursery ( Do not pour water 3-5 days before leaving the nursery to keep the soil in the hole tight). During the growth process of the seedlings, it is necessary to prevent the harm of pests. After 10 days of removing the film, it needs to be sprayed with DuPont Kangkuan and other agents (the spraying concentration is carried out according to the instructions for use) once, and then sprayed once a week before leaving the nursery.

实施例三Embodiment three

1、露天苗床准备1. Open-air seedbed preparation

同实施例一Same as Example 1

2、基质的配制2. Matrix preparation

在苗床旁边按1个苗床(10平方米)约100㎏基质准备。基质配比:细土78㎏,含水59%的腐熟木糠9㎏,有机肥8㎏,无机肥5㎏,农药0.2㎏,充分拌匀。Prepare about 100kg of substrate for 1 seedbed (10 square meters) next to the seedbed. Substrate ratio: 78kg of fine soil, 9kg of decomposed wood bran with a water content of 59%, 8kg of organic fertilizer, 5kg of inorganic fertilizer, 0.2kg of pesticide, and mix well.

3、有机肥配制3. Organic fertilizer preparation

将人和动物(猪、牛、羊、鸡等蓄禽粪便中的1种)粪便、饼肥等,使用前3个月需将所选取的粪便进行堆沤、发酵,使用时按照配制要求进行。配制时,按100㎏基质加堆沤腐熟的有机肥10㎏,充分拌匀。Human and animal manure (one of the poultry manures such as pigs, cows, sheep, chickens, etc.) manure, cake fertilizer, etc., need to be composted and fermented 3 months before use, and used in accordance with the preparation requirements. When preparing, add 10 kg of decomposed organic fertilizer to 100 kg of substrate, and mix well.

4、无机肥配制4. Inorganic fertilizer preparation

是指尿素、钾肥、过磷酸钙的混合物。在配制时,按100㎏基质加含量为46%的尿素1.5㎏、60%的氯化钾1.4㎏、16%的普通过磷酸钙1.9㎏,并将该无机肥充分混合后与土壤拌匀。Refers to the mixture of urea, potassium fertilizer and superphosphate. When preparing, add 1.5kg of 46% urea, 1.4kg of 60% potassium chloride, and 1.9kg of 16% ordinary calcium superphosphate to 100kg of substrate, and mix the inorganic fertilizer thoroughly with the soil.

5、农药5. Pesticides

采用触杀性和内吸性两种农药。触杀性的选取5%辛硫磷颗粒剂,内吸性选取3.6%杀虫双颗粒剂。每100㎏基质里添加上述农药各0.1㎏。Both contact and systemic pesticides are used. Select 5% phoxim granules for contact action, and 3.6% insecticidal double granules for systemic action. Add 0.1kg of the above pesticides to every 100kg of matrix.

6、穴盘6. Cave plate

穴盘准备:购置穴盘规格为28×54㎝,穴孔(50孔/张)规格为4—5×8—9㎝的塑料穴盘,并将穴盘平铺于苗床上。每10平方米(即1畦苗床)放置54张穴盘。Hole plate preparation: Purchase a plastic hole plate with a size of 28×54cm and a hole size (50 holes/sheet) of 4-5×8-9cm, and spread the hole plate on the seedbed. Place 54 plugs per 10 square meters (that is, 1 bed).

基质填装:将已配制好的基质填装满穴孔,再在表面覆盖2㎝厚的椰糠作为保湿材料,淋透水。Substrate filling: Fill the holes with the prepared substrate, then cover the surface with 2cm thick coconut bran as a moisturizing material, and drench with water.

基质杀菌:在移栽前3天,对穴盘基质进行淋洒杀菌剂,每平方米苗床淋65%代森锌杀菌剂500倍液10公斤,淋洒后用塑料薄膜将穴盘盖严,移苗前1天打开塑料薄膜,再浇水淋透的过程。Substrate sterilization: 3 days before transplanting, spray the fungicide on the plug matrix, spray 10 kg of 65% Zinc fungicide 500 times solution per square meter of seedbed, cover the plug tightly with a plastic film after spraying, The process of opening the plastic film one day before transplanting the seedlings, and then watering them thoroughly.

7、原种苗穴盘移栽假植7. Transplanting the original seedlings into plug trays for artificial planting

原种苗炼苗:从培养室取出1000株甘蔗脱毒原种苗,放置在移苗地附近阴凉通风处(不要淋雨,不要让太阳光照射)3天,然后剪开组培袋的袋口(若是培养瓶,则打开瓶盖),再放置2天。Hardening of original seedlings: Take out 1000 sugarcane detoxified original seedlings from the cultivation room, place them in a cool and ventilated place near the transplanting site (no rain, no sunlight) for 3 days, and then cut the bag of the tissue culture bag (if it is a culture bottle, open the bottle cap), and leave it for another 2 days.

原种苗清洗杀菌:将经过炼苗后的原种苗取出,剪去幼苗2—3㎝的叶片,放在塑料大盆里,用流动的清水将培养基清洗干净,然后用65%代森锌杀菌剂800倍液杀菌剂浸泡根系5分钟,取出在阴凉处晾干水分。Cleaning and sterilization of the original seedlings: Take out the original seedlings after hardening, cut off the 2-3cm leaves of the seedlings, put them in a large plastic pot, clean the medium with flowing water, and then use 65% Daisen Soak the root system with 800 times zinc fungicide and fungicide for 5 minutes, take it out and dry it in a cool place.

原种苗移栽:移栽时用手指轻轻扒开(深约2㎝)穴孔上层的保湿材料(木糠)后,将已清洗处理过的原种苗栽入穴孔的基质中,深约1.5—2厘米,每穴1株,然后用手指轻轻压实,淋透定根水(原种苗移栽后,需要保持基质湿润)。用2.5—3米长的竹签或铁线折成弧形,插在苗床两边,盖上遮阳网,温度低于25℃时,加盖1层塑料薄膜,5天打开淋1次水,13天撤出塑料膜【撤出塑料膜即开始淋施水肥,每6天淋施1次,第1—3次按0.3%的尿素水溶液(100㎏水加0.3㎏尿素,充分混匀)淋施,淋施时按10kg/m2喷洒;第4次按0.4%的尿素水(即100㎏水加0.4㎏尿素)按10kg/m2喷洒,以后每隔5天按此浓度及用量淋施1次,出苗前10天不再淋施水肥】,16天拆撤出遮阳网;原种苗生长60天,种苗主茎开始出现分蘖,苗长至20—30㎝高,即可出圃(出圃前3—5天不要淋水,以保持穴孔里的土团紧实)。幼苗在穴盘生长过程中,需要防止害虫的危害,在撤膜10天后需用杜邦康宽等药剂喷施(喷药浓度按使用说明执行)1次,出圃前一周再喷施1次。Transplanting the original seedlings: When transplanting, use your fingers to gently remove the moisturizing material (wood bran) on the upper layer of the hole (about 2cm deep), and then plant the cleaned original seedlings into the matrix of the hole. The depth is about 1.5-2 cm, 1 plant per hole, and then gently compacted with your fingers, drenched with root-fixing water (after transplanting the original seedlings, the substrate needs to be kept moist). Use a 2.5-3 meter long bamboo stick or iron wire to bend into an arc, insert it on both sides of the seedbed, cover with a sunshade net, and cover it with a layer of plastic film when the temperature is lower than 25°C, open it every 5 days and pour water once, 13 Withdraw the plastic film every day [withdraw the plastic film and start spraying water and fertilizer, once every 6 days, and spray with 0.3% urea aqueous solution (100kg water plus 0.3kg urea, fully mixed) for the first to third time , Spray at 10kg/ m2 when spraying; spray at 10kg/ m2 with 0.4% urea water (ie 100kg water plus 0.4kg urea) for the fourth time, and then spray at this concentration and dosage every 5 days for 1 Second, do not spray water and fertilizer 10 days before emergence], remove the sunshade net 16 days later; the original seedlings grow for 60 days, the main stems of the seedlings begin to appear tillers, and the seedlings grow to a height of 20-30 cm, and then they can go out of the nursery (out of the nursery) Do not pour water for the first 3-5 days to keep the soil in the hole tight). During the growth process of the seedlings, it is necessary to prevent the harm of pests. After 10 days of removing the film, it needs to be sprayed with DuPont Kangkuan and other agents (the spraying concentration is carried out according to the instructions for use) once, and then sprayed once a week before leaving the nursery.

移栽对比试验:Transplanting comparison test:

在备耕盖膜后的田间,取实施例一、二、三中经过穴盘假植的脱毒种苗1000株,然后按规定的株距在地膜中间用小铁铲挖一深约8—10㎝的穴,将穴盘种苗种植在穴里,填土、压实,淋定根水,采取滴灌的方式进行田间灌溉,观察记录种苗种植成活率、开始分蘖天数和拔节期,结果见表1。In the field prepared for tillage and covered with film, take 1,000 virus-free seedlings that have been artificially planted in plug trays in Examples 1, 2, and 3, and then use a small shovel to dig a depth of about 8-10 cm in the middle of the film according to the specified spacing. Plant the seedlings in the holes, fill the soil, compact them, drench the roots with water, and use drip irrigation to irrigate the field. Observe and record the survival rate of seedlings, the days of tillering and the jointing stage. The results are shown in the table 1.

在附近选择一块田地,备耕盖膜,以裸根脱毒种苗移栽作对照。取裸根脱毒种苗1000株,先将裸根苗进行浆根,然后按规定的株距在地膜中间用小铁铲挖一深约8—10㎝的穴,将种苗种植在穴里,填土、压实,淋定根水,采取滴灌的方式进行田间灌溉,观察记录种苗种植成活率、开始分蘖天数和拔节期,结果见表1。Select a nearby field, prepare for plowing and cover with film, and transplant the bare-root virus-free seedlings as a control. Take 1000 bare-root detoxified seedlings, first root the bare-rooted seedlings, and then dig a hole about 8-10 cm deep with a small shovel in the middle of the plastic film according to the specified spacing, plant the seedlings in the hole, fill The soil was compacted, and root water was drenched, and the field was irrigated by drip irrigation. The survival rate of seedlings, the days of tillering and jointing period were observed and recorded. The results are shown in Table 1.

表1甘蔗种苗移栽实验Table 1 Transplanting experiment of sugarcane seedlings

Figure BDA0000317563851
Figure BDA0000317563851

由上述结果可以看出,采用种植于穴盘的脱毒种苗进行移栽,种植成活率、开始分蘖都优于裸根脱毒种苗移栽,而且分蘖后就进入拔节期,缓苗期短,恢复生长快,对甘蔗脱毒种苗及配套栽培技术的推广应用起到了积极的推动作用。As can be seen from the above results, the use of virus-free seedlings planted in plug trays for transplanting has better planting survival rate and beginning tillering than bare-root virus-free seedlings, and after tillering, it will enter the jointing stage and slow seedling stage. It is short and recovers quickly, which has played a positive role in promoting the popularization and application of virus-free sugarcane seedlings and supporting cultivation techniques.

以上所述仅是本发明的优选实施方式,应当指出,对于本技术领域的普通技术人员来说,在不脱离本发明技术原理的前提下,还可以做出若干改进和润饰,这些改进和润饰也应视为本发明的保护范围。The above is only a preferred embodiment of the present invention, it should be pointed out that for those of ordinary skill in the art, without departing from the technical principle of the present invention, some improvements and modifications can also be made. These improvements and modifications It should also be regarded as the protection scope of the present invention.

Claims (4)

1. the former seedling plug transplantation of sugarcane detoxication is heeled in method, it is characterized in that, comprises the early-stage preparations process, transplants and heel in and management process and liquid manure pencil reason process, and its step is as follows:
1), early-stage preparations process
Comprise seedbed preparatory process, matrix preparation process, the filling of cave disk base material and sterilization process;
Seedbed preparatory process: selected nursery lot is carried out rotary tillage, dark plough, rotary tillage, plays furrow; Spread fertilizer over the fields insecticide in the seedbed, and insecticide and furrow face fine earth are mixed thoroughly, raked, with sprouting preceding herbicide spray;
Matrix preparation process: fine earth, material which can retain moisture, fertilizer, inorganic fertilizer and insecticide are fully mixed thoroughly and made, wherein the ratio of each component is by weight: fine earth 70~80, material which can retain moisture 8~12, fertilizer 8~12, inorganic fertilizer 4~6, insecticide 0.1~0.3; Described material which can retain moisture refers to that water content is 50~60% coconut palm chaff, the bagasse that becomes thoroughly decomposed or wooden chaff; Described fertilizer refers to the human or animal's of becoming thoroughly decomposed ight soil, cake fertilizer; Described inorganic fertilizer refers to the mixture of urea, potash fertilizer and superphosphate, wherein urea by weight: potash fertilizer: superphosphate=6~7:8~10:3~4;
The cave disk base material is loaded and sterilization process: the acupoint selection dish lies against the cave dish on the seedbed according to the seedbed specification then; The matrix for preparing is loaded full hole, cave, again at the thick material which can retain moisture of surface coverage 1.5~2.5 ㎝, the water of drenching; Before transplanting 2~3 days, with plastic film that the cave disk cover is tight then with bactericide sprinkle cave disk base material, transplant seedlings and opened plastic film in preceding 1 day, rewater and drench;
2), transplanting is heeled in and management process
The former seedling of detoxification of planting in group training device was placed on the shady and cool ventilation place 2~3 days, opens group training device then and place again and carried out hardening in 1~2 day; To take out through the former seedling of the detoxification after the hardening, cut off the blade of seedling 2~3 ㎝, medium will be cleaned up, soak 3~5 minutes with 800 times of liquid of bactericide again, and take out and dry moisture in the cool; Get the cave dish of loading matrix, push after the material which can retain moisture on upper strata, hole, cave the former seedling of detoxification that cleaning treatment crosses aside and plant in the matrix in hole, cave, dark 1.5~2 centimetres, 1 strain of every cave, the normal root water of drenching; The sunshade net is set above the seedbed, when temperature is lower than 25 ℃, adds a cover layer of plastic film, opened in 3~5 days and to drench 1 water, withdrew from plastic foil in 13~15 days, tore open in 15~20 days and to withdraw from the sunshade net; The former seedling of detoxification was grown 45~75 days in the dish of cave again, and the seedling stem begins to occur tillering, and seedling grows to 20~30 ㎝ height, can go out the garden;
3), liquid manure pencil reason process
Comprise water management, Nutrition Management, three aspects of Pest Management;
Water management: the former seedling of detoxification need to keep matrix moistening behind plug transplantation, withdraws from behind the plastic foil matrix and keeps moist, former seedling not occur wilting;
Nutrition Management: after withdrawing from plastic foil, begin to drench and execute liquid manure, pourings in per 4~6 days executed 1 time, presses 0.3% aqueous solution of urea for the 1st~3 time and presses 10kg/m 2Spray; The 4th is pressed 10kg/m by 0.4% aqueous solution of urea 2Spray, drench by this concentration and consumption later on and execute, emerge no longer to drench in preceding 10 days and execute liquid manure;
Pest Management: seedling need carry out the pest control management in cave dish process of growth, needs with medicament to spray after 10 days 1 time removing film, goes out the process that the garden sprays 1 the last week again.
2. the former seedling plug transplantation of sugarcane detoxication according to claim 1 is heeled in method, it is characterized in that: the specification in described seedbed is wide 1 meter, long 10 meters.
3. the former seedling plug transplantation of sugarcane detoxication according to claim 1 is heeled in method, it is characterized in that: each components by weight of matrix is: fine earth 70, material which can retain moisture 10, fertilizer 7, inorganic fertilizer 3, insecticide 0.2.
4. the former seedling plug transplantation of sugarcane detoxication according to claim 1 is heeled in method, it is characterized in that: described cave dish specification is 28 * 54 ㎝, and cave hole gauge lattice are 4~5 * 8~9 ㎝ or 6~7 * 8~9 ㎝.
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Cited By (7)

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Publication number Priority date Publication date Assignee Title
CN103563616A (en) * 2013-10-10 2014-02-12 来宾市兴宾区科学技术局 Method for improving survival rate of temporarily planted sugarcane tissue culture seedling
CN104756745A (en) * 2015-04-27 2015-07-08 中国热带农业科学院热带生物技术研究所 Weeding-free transplanting method for sugarcane detoxification original seedling
CN104756745B (en) * 2015-04-27 2017-09-19 中国热带农业科学院热带生物技术研究所 A kind of sugarcane detoxication original seed seedling exempts careless method for transplanting
CN106332648A (en) * 2016-08-30 2017-01-18 广西壮族自治区农业科学院甘蔗研究所 Single-plant temporary planting method of sugarcane tissue culture rooting bottle seedling
CN106478190A (en) * 2016-11-03 2017-03-08 中国热带农业科学院南亚热带作物研究所 A kind of Fructus Solani melongenae soilless raising seedling substrate and preparation method thereof
CN106478190B (en) * 2016-11-03 2020-03-27 中国热带农业科学院南亚热带作物研究所 Soilless seedling culture substrate for eggplants and preparation method thereof
CN112314378A (en) * 2020-10-26 2021-02-05 广西壮族自治区农业科学院 An efficient method for transplanting sugarcane test-tube seedlings

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