CN105418172B - The preparation method of biological organic fertilizer - Google Patents
The preparation method of biological organic fertilizer Download PDFInfo
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- CN105418172B CN105418172B CN201610012339.2A CN201610012339A CN105418172B CN 105418172 B CN105418172 B CN 105418172B CN 201610012339 A CN201610012339 A CN 201610012339A CN 105418172 B CN105418172 B CN 105418172B
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The present invention provides a kind of preparation methods of biological organic fertilizer, the preparation method includes providing fermentation substrate, stacks the step of fermentation substrate and fermenting substrate, wherein, described the step of stacking fermentation substrate, includes the ratio of the fermented liquid microbial bacterial agent according to 0.9 1.1 L of every 1000 kg fermentation substrates addition, the fermentation substrate is uniformly mixed with the fermented liquid microbial bacterial agent, stack into the rectangle fermentation substrate heap of high 0.5 0.8 m, wherein, the fermented liquid microbial bacterial agent includes following components：Bacillus subtilis bacterium solution, Bacillus licheniformis liquid, saccharomyces cerevisiae bacterium solution, Bacillus acidi lactici bacterium solution and aspergillus niger bacterium solution.The preparation method of above-mentioned biological organic fertilizer provided by the invention, which can not only be realized, to turn waste into wealth, when can also solve organic solid castoff recycling treatment, the decomposition high molecular weight protein and crude fibre ability of generally existing are weak, and nitrogen is easily lost, and lead to the problem of the pollution environment such as pungent peculiar smell.
Description
Technical field
The present invention relates to fertilizer field, more particularly, to a kind of fermented liquid microbial bacterial agent and preparation method thereof and fermentation
Method.
Background technology
At present, China is in terms of crop-planting, mostly using chemical fertilizer, although having in terms of increasing production, increasing income certain
It rents, but is used for a long time, problems with can be generated：
1st, soil is destroyed, on the contrary the organic matter content in soil reduces, and causes nutritional ingredient out of proportion, and soil occurs
Phenomena such as being acidified, is hardened influences the yield of crop；
2nd, agroecological environment deteriorates, and chemical fertilizer is applied as behind farmland, and practical efficiency averagely only has 30%, and major part is with farmland
Draining is flowed into rivers, is polluted environment, is endangered health of people.
Therefore, Current Domestic is outer for all trying to explore in terms of organic solid castoff recycling treatment, while
There is a series of related microorganisms microbial inoculum to ferment for organic solid castoff.It is but general during recycling treatment of fermenting
Store-through is in decomposition high molecular weight protein and crude fibre ability is weak and nitrogen is easily lost, and generates the problems such as being pungent peculiar smell.
Invention content
It is therefore an object of the present invention to provide a kind of preparation method of biological organic fertilizer, can not only realize turns waste into wealth,
Can also solve in the prior art utilize organic solid castoff recycling treatment when, the decomposition high molecular weight protein of generally existing and
Crude fibre ability is weak, and nitrogen is easily lost, and leads to the problem of the pollution environment such as pungent peculiar smell.
The technical solution that the present invention takes is as follows：
A kind of preparation method of biological organic fertilizer, includes the following steps：
Fermentation substrate is provided：Organic waste of the water content between 60%-70% is provided, as fermentation substrate；
Stack fermentation substrate：The fermented liquid microbial bacterial agent of 0.9-1.1 L is added according to every 1000 kg fermentation substrates
The fermentation substrate with the fermented liquid microbial bacterial agent is uniformly mixed, stacks into the square of high 0.5-0.8 m by ratio
Shape fermentation substrate heap, wherein, the fermented liquid microbial bacterial agent includes following components：Bacillus subtilis bacterium solution, lichens bud
Spore bacillus bacterium solution, saccharomyces cerevisiae bacterium solution, Bacillus acidi lactici bacterium solution and aspergillus niger bacterium solution；
Fermenting substrate：The rectangle fermentation substrate piles up 48 DEG C -68 DEG C and ferments, until the fermentation substrate it is loose and
Odorless.
Wherein, " water content " herein refers to the mass percent that the moisture in organic waste occupies.
Based on above-mentioned, the organic waste is feces of livestock and poultry, one or more of stalk, edible fungi leftovers.
Based on above-mentioned, the fermentation substrate includes the organic waste of following mass percent：Feces of livestock and poultry 35%-45%,
Stalk 27.5%-32.5%, edible fungi leftovers 27.5%-32.5%, and of the stalk and edible fungi leftovers for 3-5 mm
Grain.
Based on above-mentioned, the fermented liquid microbial bacterial agent includes the component of volumes below percentage：Bacillus subtilis
Bacterium solution 40%-50%, Bacillus licheniformis liquid 12%-18%, saccharomyces cerevisiae bacterium solution 16%-24%, Bacillus acidi lactici bacterium solution 7%-9% and black
Aspergillus bacterium solution 10%-14%, wherein, the bacillus subtilis bacterium solution, the Bacillus licheniformis liquid, the S. cervisiae
Hundred million cfu/ml of a concentration of 9-11 of liquid, the Bacillus acidi lactici bacterium solution and the aspergillus niger bacterium solution.
Based on above-mentioned, the preparation method of the fermented liquid microbial bacterial agent includes the following steps：
Activation culture：By Bacillus subtilis strain, Bacillus licheniformis strain, saccharomyces cerevisiae strain, Bacillus acidi lactici bacterium
Kind, aspergillus niger strain are inoculated into corresponding culture medium and carry out activation culture respectively, respectively obtain generation bacterial strain；
Expand culture：The generation bacterial strain of the bacillus subtilis and the generation bacterial strain of the bacillus licheniformis are distinguished
It is inoculated into broth agar culture medium and is cultivated, respectively obtain bacillus subtilis bacterium solution and Bacillus licheniformis liquid；It will
The generation bacterial strain of the saccharomyces cerevisiae, the generation bacterial strain of the aspergillus niger are inoculated into PDA culture medium are cultivated respectively, respectively
Obtain saccharomyces cerevisiae bacterium solution and aspergillus niger bacterium solution；It will be trained in the generation inoculation to MRS culture mediums of the Bacillus acidi lactici
It supports and obtains Bacillus acidi lactici bacterium solution.
Prepare microbial inoculum：To the bacillus subtilis bacterium solution, the Bacillus licheniformis liquid, the S. cervisiae
Liquid, the Bacillus acidi lactici bacterium solution and the aspergillus niger bacterium solution are mixed, and obtain the fermented liquid microbial bacterial agent.
Based on above-mentioned, the step of activation culture, includes：Using broth agar culture medium respectively to the withered grass gemma
Bacillus species and the Bacillus licheniformis strain carry out activation culture, respectively obtain the generation bacterial strain of the bacillus subtilis
With the generation bacterial strain of the bacillus licheniformis；Using PDA culture medium respectively to the saccharomyces cerevisiae strain and the aspergillus niger
Strain carries out activation culture, respectively obtains the generation bacterial strain of the saccharomyces cerevisiae and the generation bacterial strain of the aspergillus niger；Using MRS
Culture medium carries out activation culture to the Lactobacillus species, obtains the Bacillus acidi lactici generation bacterial strain.
Based on above-mentioned, the cultivation temperature in the activation culture step is 29-31 DEG C, and the activation culture time is small for 20-28
When.
Based on above-mentioned, the cultivation temperature in the incubation step that spreads cultivation is 27-29 DEG C, and the incubation time that spreads cultivation is small for 40-56
When.
Wherein, the bacillus subtilis bacterium solution cultivated in the activation culture step, the lichens gemma bar
Bacterium bacterium solution, the saccharomyces cerevisiae bacterium solution, the Bacillus acidi lactici bacterium solution and the aspergillus niger bacterium solution hundred million cfu/ml of a concentration of 9-11；
In described the step of preparing microbial inoculum, each bacterium solution is mixed according to volumes below percentage：Bacillus subtilis bacterium solution 40%-50%,
Bacillus licheniformis liquid 12%-18%, saccharomyces cerevisiae bacterium solution 16%-24%, Bacillus acidi lactici bacterium solution 7%-9% and aspergillus niger bacterium solution 10%-
14%。
Based on above-mentioned, in the step of fermenting substrate, the fermentation time of the rectangle fermentation substrate heap is 8-10 days.
The liquid microbe microbial inoculum that five kinds of microorganism mixing obtain in the present invention can significantly improve animal dung compared with the prior art
Just, stalk, edible fungi leftovers fermentation efficiency significantly improve feces of livestock and poultry, stalk, edible fungi leftovers recycling
Rate.There is good promotional value in agricultural, livestock and poultry cultivation.Solve environmental pollution and the recycling of solid waste simultaneously
The problem of utilization rate is low.
Compared with prior art, the preparation method of biological organic fertilizer provided by the invention mainly utilizes the micro- life of fermented liquid
Object microbial inoculum carries out fermentation reaction to organic solid castoff and biological organic fertilizer is made, and turns waste into wealth, method is fairly simple, easily
In application；Moreover, the fermented liquid microbial bacterial agent that the present invention uses is a kind of main including bacillus subtilis, lichens gemma
Five kinds of microorganisms such as bacillus, saccharomyces cerevisiae, Bacillus acidi lactici and aspergillus niger compound the efficient cellulase-producing formed, protease and remove
The function and service microbial inoculum of smelly active material, division of labor effect is clear and definite between each microorganism in the fermented liquid microbial bacterial agent,
There is efficient degradation performance for the high molecular weight protein and crude fibre that are difficult to degrade, stablize by force, so, it can be quick with high-efficiency environment friendly
The cellulose, high molecular weight protein, various complexings that ground decomposes the organic wastes such as feces of livestock and poultry, stalk, edible fungi leftovers are micro
Element forms the efficient biologic-organic fertilizer rich in beneficial microbe, organic matter, nitrogen, phosphorus, potassium and trace element；And cause the life
It effectively improves the organic matter in soil beneficial to viable bacteria, decomposition enzyme and bioactie agent containing a large amount of in organic fertilizer, subtract
The generation of few diseases and pests of agronomic crop.Therefore, the preparation method of biological organic fertilizer provided by the invention there is fermentation initial stage to heat up fast
Speed, high-temperature duration is long, and protease and cellulase activity are high and stable, and fermentation time is short, and enzyme activity is strong, viable count is high, removes
The features such as smelly effect is good；And in the preparation process of the biological organic fertilizer, not only feces of livestock and poultry, stalk, edible mushroom are got a foothold
The organic wastes such as material become biological organic fertilizer, turn waste into wealth, while free from extraneous odour generates in whole preparation process, environmental-friendly,
Solves the problem of environmental pollution that existing organic waste is also easy to produce pungent peculiar smell etc. in processing procedure.
Further, the fermented liquid microbial bacterial agent used in the preparation method of biological organic fertilizer provided by the invention is main
By 45% bacillus subtilis bacterium solution, 15% Bacillus licheniformis liquid, 20% saccharomyces cerevisiae bacterium solution, 8% Bacillus acidi lactici bacterium solution, 12%
Aspergillus niger bacterium solution composition multi-functional composite bacteria agent, the fermented liquid microbial bacterial agent have structure is excellent, effect is steady, enzyme activity is strong,
The features such as viable count is high can rapidly decompose the organic wastes such as feces of livestock and poultry, stalk, edible fungi leftovers with more efficient environmental protection
Various celluloses, high molecular weight protein, it is various complexing trace element, formed rich in beneficial microbe, organic matter, nitrogen, phosphorus, potassium and
The efficient biologic-organic fertilizer of trace element, and pungent peculiar smell will not be generated.
Specific embodiment
Below by specific embodiment, technical scheme of the present invention is described in further detail.
Embodiment 1
The embodiment of the present invention 1 provides a kind of preparation method of biological organic fertilizer, includes the following steps：
Fermentation substrate is provided：The chicken manure organic waste that water content is 60% is provided, as fermentation substrate；
Stack fermentation substrate：The ratio of the fermented liquid microbial bacterial agent of 0.9 L is added according to every 1000 kg fermentation substrates
Example, after the fermentation substrate is mixed with the fermented liquid microbial bacterial agent, then with 3 mixings of organic fertilizer turner,
And stack into high 0.5 m rectangle fermentation substrate heap；
Fermenting substrate：The rectangle fermentation substrate piles up 48-50 DEG C and ferments, and fermentation substrate is loose after fermentation 8 days, nothing
Terminate to ferment during stink, obtain biological organic fertilizer.
Wherein, the fermented liquid microbial bacterial agent used in the present embodiment includes the component of volumes below percentage：
Bacillus subtilis bacterium solution 40%, Bacillus licheniformis liquid 18%, saccharomyces cerevisiae bacterium solution 23%, Bacillus acidi lactici bacterium solution 7% and black song
Mould liquid 10%, wherein, the bacillus subtilis bacterium solution, the Bacillus licheniformis liquid, the saccharomyces cerevisiae bacterium solution, institute
State a concentration of 900,000,000 cfu/ml of Bacillus acidi lactici bacterium solution and the aspergillus niger bacterium solution.The preparation side of the fermented liquid microbial bacterial agent
Method includes the following steps：
Activation culture：By Bacillus subtilis strain, Bacillus licheniformis strain, saccharomyces cerevisiae strain, Bacillus acidi lactici bacterium
Kind, aspergillus niger strain be inoculated into respectively in corresponding culture medium in 29 DEG C of activation cultures 28 hours, respectively obtain generation bacterial strain；
Expand culture：The generation bacterial strain of the bacillus subtilis and the generation bacterial strain of the bacillus licheniformis are distinguished
It is inoculated into broth agar culture medium, spread cultivation culture 56 hours at 27 DEG C, respectively obtains the withered grass gemma of a concentration of 900,000,000 cfu/ml
Bacillus bacterium solution and Bacillus licheniformis liquid；The generation bacterial strain of the generation bacterial strain of the saccharomyces cerevisiae, the aspergillus niger is distinguished
It is inoculated into PDA culture medium, spread cultivation culture 56 hours at 27 DEG C, respectively obtains the saccharomyces cerevisiae bacterium solution of a concentration of 900,000,000 cfu/ml
With aspergillus niger bacterium solution；In the generation inoculation to MRS culture mediums of the Bacillus acidi lactici, will spread cultivation culture 56 hours at 27 DEG C,
Obtain the Bacillus acidi lactici bacterium solution of a concentration of 900,000,000 cfu/ml；
Prepare microbial inoculum：The formula of above-mentioned fermented liquid microbial bacterial agent according to being used in the present embodiment is mixed, and is obtained
To above-mentioned fermented liquid microbial bacterial agent.
Using method as defined in organic fertilizer NY525-2012 standards to as made from preparation method provided in this embodiment
The biological organic fertilizer is tested, wherein, the mass percentage of the key component in the biological organic fertilizer is as follows：It is organic
Matter 70%, purity nitrogen 2.11%, phosphorus（P2O5）2.07%, potassium（K2O）0.85%, meet the standard of organic fertilizer, conduct can be used directly to
Chicken manure organic fertilizer is applied.
Embodiment 2
The embodiment of the present invention 2 provides a kind of preparation method of biological organic fertilizer, includes the following steps：
Fermentation substrate is provided：The organic waste that water content is 63% is provided, as fermentation substrate, wherein, the fermentation bottom
Object is made of the organic waste of following mass percent：60% pig manure and 40% stalk, and under the stalk and edible mushroom
Heel is the particle of 3-5 mm；
Stack fermentation substrate：The ratio of the fermented liquid microbial bacterial agent of 0.95 L is added according to every 1000 kg fermentation substrates
Example, after the fermentation substrate is mixed with the fermented liquid microbial bacterial agent, then with 4 mixings of organic fertilizer turner,
And stack into high 0.6-0.7 m rectangle fermentation substrate heap；
Fermenting substrate：The rectangle fermentation substrate piles up 52-54 DEG C and ferments, and fermentation substrate is loose after fermentation 9 days, nothing
Terminate to ferment during stink, obtain biological organic fertilizer.
Wherein, the fermented liquid microbial bacterial agent used in the present embodiment includes the component of volumes below percentage：
Bacillus subtilis bacterium solution 42%, Bacillus licheniformis liquid 16%, saccharomyces cerevisiae bacterium solution 22%, Bacillus acidi lactici bacterium solution 7.5% and black
Aspergillus bacterium solution 12.5%, wherein, a concentration of 9.2 hundred million cfu/ml, the Bacillus licheniformis of the bacillus subtilis bacterium solution
A concentration of 9.5 hundred million cfu/ml of liquid, a concentration of 1,000,000,000 cfu/ml of the saccharomyces cerevisiae bacterium solution, the Bacillus acidi lactici bacterium solution it is dense
Spend a concentration of 9.3 hundred million cfu/ml for 9.8 hundred million cfu/ml and the aspergillus niger bacterium solution.The above-mentioned fermented liquid that the present embodiment uses
The preparation method of microbial bacterial agent, the preparation method of fermented liquid microbial bacterial agent used with embodiment 1 is essentially identical, different
Part is：
In the step of activation culture, the Bacillus subtilis strain and Bacillus licheniformis strain are inoculated with respectively
Into broth agar culture medium, in 30 DEG C of activation cultures 24 hours, the generation bacterial strain and lichens of bacillus subtilis are respectively obtained
The generation bacterial strain of bacillus；Saccharomyces cerevisiae strain and aspergillus niger strain are inoculated into PDA culture medium respectively, in 29 DEG C of activation
Culture 26 hours, respectively obtains the generation bacterial strain of saccharomyces cerevisiae and the generation bacterial strain of aspergillus niger；Lactobacillus species are inoculated into
In MRS culture mediums, in 29 DEG C of activation cultures 24 hours, Bacillus acidi lactici generation bacterial strain is obtained；
In described the step of expanding culture, the bacillus subtilis, the bacillus licheniformis, the wine brewing ferment
The cultivation temperature that spreads cultivation of female, described aspergillus niger and the respective generation bacterial strain of the Bacillus acidi lactici is 28 DEG C, and the incubation time that spreads cultivation is
50 hours, a concentration of 9.2 hundred million cfu/ml, the Bacillus licheniformis liquid of the bacillus subtilis bacterium solution finally obtained
A concentration of 9.5 hundred million cfu/ml, a concentration of 1,000,000,000 cfu/ml of the saccharomyces cerevisiae bacterium solution, the Bacillus acidi lactici bacterium solution concentration
For 9.8 hundred million cfu/ml and a concentration of 9.3 hundred million cfu/ml of the aspergillus niger bacterium solution；
In described the step of preparing microbial inoculum, carried out according to the formula of fermented liquid microbial bacterial agent that the present embodiment uses
Mixing.
Using method as defined in organic fertilizer NY525-2012 standards to being obtained by preparation method provided in this embodiment
The biological organic fertilizer is tested, wherein, the mass percentage of the key component in the biological organic fertilizer is as follows：It is organic
Matter 65%, purity nitrogen 1.97%, phosphorus（P2O5）2.31%, potassium（K2O）1.85%, meet the standard of organic fertilizer, conduct can be used directly to
Swine manure is applied.
Embodiment 3
The embodiment of the present invention 3 provides a kind of preparation method of biological organic fertilizer, includes the following steps：
Fermentation substrate is provided：The organic waste that water content is 65% is provided, as fermentation substrate, wherein, the fermentation bottom
Object is made of the organic waste of following mass percent：Feces of livestock and poultry 40%, stalk 30%, edible fungi leftovers 30%, and it is described
Stalk and the particle that edible fungi leftovers are 3-5 mm；
Stack fermentation substrate：The ratio of the fermented liquid microbial bacterial agent of 0.9 L is added according to every 1000 kg fermentation substrates
Example, after the fermentation substrate is mixed with the fermented liquid microbial bacterial agent, then with 3 mixings of organic fertilizer turner,
And stack into high 0.7-0.8 m rectangle fermentation substrate heap；
Fermenting substrate：The rectangle fermentation substrate piles up 57-59 DEG C and ferments, and fermentation substrate is loose after fermentation 7 days, nothing
Terminate to ferment during stink, obtain biological organic fertilizer.
Wherein, the fermented liquid microbial bacterial agent used in the present embodiment includes the component of volumes below percentage：
Bacillus subtilis bacterium solution 45%, Bacillus licheniformis liquid 15%, saccharomyces cerevisiae bacterium solution 20%, Bacillus acidi lactici bacterium solution 8% and black song
Mould liquid 12%, wherein, the bacillus subtilis bacterium solution, the Bacillus licheniformis liquid, the saccharomyces cerevisiae bacterium solution, institute
State a concentration of 1,000,000,000 cfu/ml of Bacillus acidi lactici bacterium solution and the aspergillus niger bacterium solution.The above-mentioned fermented liquid that the present embodiment uses is micro-
The preparation method of bacteria agent, the preparation method of fermented liquid microbial bacterial agent used with embodiment 2 is essentially identical, difference
Be in：
In the step of activation culture, the activation culture temperature of described five kinds of microorganism fungus kinds is 30 DEG C, living
It is 24 hours to change incubation time；
In described the step of expanding culture, the generation bacterial strains of the five kinds of micro- raw bacterium cultivation temperature that spreads cultivation respectively is 28
DEG C, the incubation time that spreads cultivation is 48 hours, and the concentration of the bacterium solution of above-mentioned five kinds micro- raw bacterium finally obtained is 1,000,000,000 cfu/ml；
In described the step of preparing microbial inoculum, carried out according to the formula of fermented liquid microbial bacterial agent that the present embodiment uses
Mixing.
Using method as defined in organic fertilizer NY525-2012 standards to obtained by by preparation method provided in this embodiment
Biological organic fertilizer tested, wherein, the mass percentage of the key component in the biological organic fertilizer is as follows：Organic matter
63%, purity nitrogen 1.82%, phosphorus（P2O5）1.93%, potassium（K2O）2.13%, pH value 5.5-6.5 meet the standard of organic fertilizer, can be with
It is used directly to as organic fertilizer application.
Embodiment 4
The embodiment of the present invention 4 provides a kind of preparation method of biological organic fertilizer, includes the following steps：
Fermentation substrate is provided：The chicken manure organic waste that water content is 70% is provided, as fermentation substrate, wherein, it is described
Fermentation substrate is made of the organic waste of following mass percent：Feces of livestock and poultry 35%, stalk 32.5%, edible fungi leftovers
32.5%, and the particle of the stalk and edible fungi leftovers for 3-5 mm；
Stack fermentation substrate：The ratio of the fermented liquid microbial bacterial agent of 1.1 L is added according to every 1000 kg fermentation substrates
Example, after the fermentation substrate is mixed with the fermented liquid microbial bacterial agent, then with 5 mixings of organic fertilizer turner,
And stack into high 0.6-0.7 m rectangle fermentation substrate heap；
Fermenting substrate：The rectangle fermentation substrate piles up 66-68 DEG C and ferments, and fermentation substrate is loose after fermentation 8 days, nothing
Terminate to ferment during stink, obtain biological organic fertilizer.
Wherein, the fermented liquid microbial bacterial agent used in the present embodiment includes the component of volumes below percentage：
Bacillus subtilis bacterium solution 48%, Bacillus licheniformis liquid 14%, saccharomyces cerevisiae bacterium solution 18%, Bacillus acidi lactici bacterium solution 9% and black song
Mould liquid 11%, wherein, a concentration of 10.2 hundred million cfu/ml, the Bacillus licheniformis liquid of the bacillus subtilis bacterium solution
A concentration of 1,000,000,000 cfu/ml, a concentration of 10.5 hundred million cfu/ml of the saccharomyces cerevisiae bacterium solution, the Bacillus acidi lactici bacterium solution it is dense
Spend a concentration of 10.1 hundred million cfu/ml for 9.9 hundred million cfu/ml and the aspergillus niger bacterium solution.The above-mentioned zymotic fluid that the present embodiment uses
The preparation method of body microbial bacterial agent, the preparation method of fermented liquid microbial bacterial agent used with embodiment 2 is essentially identical, no
It is with part：
In the step of activation culture, the activation culture temperature of described five kinds of microorganism fungus kinds is 31 DEG C, living
It is 20 hours to change incubation time；
In described the step of expanding culture, the generation bacterial strains of the five kinds of micro- raw bacterium cultivation temperature that spreads cultivation respectively is 29
DEG C, the incubation time that spreads cultivation is 40 hours, and the five of the concentration kinds of micro- raw bacterium bacterium solutions that the present embodiment finally obtained uses；
In described the step of preparing microbial inoculum, carried out according to the formula of fermented liquid microbial bacterial agent that the present embodiment uses
Mixing.
Using method as defined in organic fertilizer NY525-2012 standards to utilizing the micro- life of fermented liquid provided in this embodiment
The biological organic fertilizer that object microbial inoculum ferments to chicken manure is tested, wherein, it is main in the biological organic fertilizer
The mass percentage of component is as follows：Organic matter 61.2%, purity nitrogen 2.31%, phosphorus（P2O5）2.81%, potassium（K2O）3.43%, pH value is
6-6.8 meets the standard of organic fertilizer, can be used directly to as organic fertilizer application.
Embodiment 5
The embodiment of the present invention 5 provides a kind of preparation method of biological organic fertilizer, includes the following steps：
Fermentation substrate is provided：The chicken manure organic waste that water content is 67% is provided, as fermentation substrate, wherein, it is described
Fermentation substrate is made of the organic waste of following mass percent：Feces of livestock and poultry 45%, stalk 27.5%, edible fungi leftovers
27.5%, and the particle of the stalk and edible fungi leftovers for 3-5 mm；
Stack fermentation substrate：The ratio of the fermented liquid microbial bacterial agent of 1 L is added according to every 1000 kg fermentation substrates,
After the fermentation substrate is mixed with the fermented liquid microbial bacterial agent, then with 4 mixings of organic fertilizer turner, and heap
Put into the rectangle fermentation substrate heap of high 0.5-0.6 m；
Fermenting substrate：The rectangle fermentation substrate piles up 48-52 DEG C and ferments, and fermentation substrate is loose after fermentation 10 days,
Terminate to ferment during odorless, obtain biological organic fertilizer.
Wherein, the fermented liquid microbial bacterial agent used in the present embodiment includes the component of volumes below percentage：
Bacillus subtilis bacterium solution 50%, Bacillus licheniformis liquid 12%, saccharomyces cerevisiae bacterium solution 16%, Bacillus acidi lactici bacterium solution 8% and black song
Mould liquid 14%, wherein, the bacillus subtilis bacterium solution, the Bacillus licheniformis liquid, the saccharomyces cerevisiae bacterium solution, institute
State a concentration of 1,100,000,000 cfu/ml of Bacillus acidi lactici bacterium solution and the aspergillus niger bacterium solution.
The preparation method of above-mentioned fermented liquid microbial bacterial agent that the present embodiment uses, the zymotic fluid used with embodiment 3
The preparation method of body microbial bacterial agent is essentially identical, the difference lies in：In described the step of expanding culture, finally obtain
The five of the concentration kinds of micro- raw bacterium bacterium solutions that the present embodiment uses；In described the step of preparing microbial inoculum, make according to the present embodiment
It is mixed with the formula of the fermented liquid microbial bacterial agent of offer.
Using method as defined in organic fertilizer NY525-2012 standards to obtained by by preparation method provided in this embodiment
The biological organic fertilizer tested, wherein, the mass percentage of the key component in the biological organic fertilizer is as follows：Have
Machine matter 65.7%, purity nitrogen 1.67%, phosphorus（P2O5）1.81%, potassium（K2O）2.17%, pH value 7-8 meet the standard of organic fertilizer, can
To be used directly to as organic fertilizer application.
Finally it should be noted that：The above embodiments are merely illustrative of the technical scheme of the present invention and are not intended to be limiting thereof；To the greatest extent
The present invention is described in detail with reference to preferred embodiments for pipe, those of ordinary skills in the art should understand that：Still
It can modify to the specific embodiment of the present invention or equivalent replacement is carried out to some technical characteristics；Without departing from this hair
The spirit of bright technical solution should all cover in the claimed technical solution range of the present invention.
Claims (8)
1. a kind of preparation method of biological organic fertilizer, includes the following steps：
Fermentation substrate is provided：Organic waste of the water content between 60%-70% is provided, as fermentation substrate；
Stack fermentation substrate：The ratio of the fermented liquid microbial bacterial agent of 0.9-1.1 L is added according to every 1000kg fermentation substrates,
The fermentation substrate with the fermented liquid microbial bacterial agent is uniformly mixed, stacks into the rectangle hair of high 0.5-0.8 m
Ferment substrate heap, wherein, the fermented liquid microbial bacterial agent is made of the component of volumes below percentage：Bacillus subtilis bacterium
Liquid 40%-50%, Bacillus licheniformis liquid 12%-18%, saccharomyces cerevisiae bacterium solution 16%-24%, Bacillus acidi lactici bacterium solution 7%-9% and black song
Mould liquid 10%-14%, wherein, the bacillus subtilis bacterium solution, the Bacillus licheniformis liquid, the S. cervisiae
Hundred million cfu/ml of a concentration of 9-11 of liquid, the Bacillus acidi lactici bacterium solution and the aspergillus niger bacterium solution；
Fermenting substrate：The rectangle fermentation substrate piles up 48 DEG C -68 DEG C and ferments, until the fermentation substrate is loose and odorless
Taste.
2. the preparation method of biological organic fertilizer according to claim 1, which is characterized in that the fermented liquid microbial bacteria
The preparation method of agent, includes the following steps：
Activation culture：By Bacillus subtilis strain, Bacillus licheniformis strain, saccharomyces cerevisiae strain, Lactobacillus species, black
Aspergillus strain, which is inoculated into respectively in corresponding culture medium, carries out activation culture, respectively obtains generation bacterial strain；
Expand culture：The generation bacterial strain of the generation bacterial strain of the bacillus subtilis and the bacillus licheniformis is inoculated with respectively
It is cultivated into broth agar culture medium, respectively obtains the bacillus subtilis bacterium solution and Bacillus licheniformis liquid；
The generation bacterial strain of the generation bacterial strain of the saccharomyces cerevisiae, the aspergillus niger is inoculated into PDA culture medium respectively and is cultivated, point
The saccharomyces cerevisiae bacterium solution and aspergillus niger bacterium solution are not obtained；By the generation inoculation of the Bacillus acidi lactici to MRS culture mediums
In cultivated, obtain the Bacillus acidi lactici bacterium solution；
Prepare microbial inoculum：Component described in accordance with the claim 1, to the bacillus subtilis bacterium solution, the bacillus licheniformis
Bacterium solution, the saccharomyces cerevisiae bacterium solution, the Bacillus acidi lactici bacterium solution and the aspergillus niger bacterium solution are mixed, and obtain the zymotic fluid
Body microbial bacterial agent.
3. the preparation method of biological organic fertilizer according to claim 2, which is characterized in that the step of activation culture wraps
It includes：The Bacillus subtilis strain and the Bacillus licheniformis strain are activated respectively using broth agar culture medium
Culture, respectively obtains the generation bacterial strain of the bacillus subtilis and the generation bacterial strain of the bacillus licheniformis；It is trained using PDA
Foster base carries out activation culture to the saccharomyces cerevisiae strain and the aspergillus niger strain respectively, respectively obtains the saccharomyces cerevisiae
The generation bacterial strain of generation bacterial strain and the aspergillus niger；Activation culture is carried out to the Lactobacillus species using MRS culture mediums, is obtained
To the Bacillus acidi lactici generation bacterial strain.
4. the preparation method of biological organic fertilizer according to claim 2, which is characterized in that in the activation culture step
Cultivation temperature is 29-31 DEG C, and the activation culture time is 20-28 hours.
5. the preparation method of biological organic fertilizer according to claim 2, which is characterized in that in the incubation step that spreads cultivation
Cultivation temperature is 27-29 DEG C, and the incubation time that spreads cultivation is 40-56 hours.
6. the preparation method of biological organic fertilizer according to claim 1, which is characterized in that the organic waste is livestock and poultry
One or more of excrement, stalk, edible fungi leftovers.
7. the preparation method of biological organic fertilizer according to claim 1, which is characterized in that the fermentation substrate include with
The organic waste of lower mass percent：Feces of livestock and poultry 35%-45%, stalk 27.5%-32.5%, edible fungi leftovers 27.5%-
32.5%, and the particle of the stalk and edible fungi leftovers for 3-5 mm.
8. the preparation method of biological organic fertilizer according to claim 1, which is characterized in that the fermenting substrate the step of
In, the fermentation time of the rectangle fermentation substrate heap is 8-10 days.
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