CN108314585A - Agriculture probiotics conversion stalk and the method for cultivating excrement production biological organic fertilizer - Google Patents
Agriculture probiotics conversion stalk and the method for cultivating excrement production biological organic fertilizer Download PDFInfo
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- CN108314585A CN108314585A CN201810214226.XA CN201810214226A CN108314585A CN 108314585 A CN108314585 A CN 108314585A CN 201810214226 A CN201810214226 A CN 201810214226A CN 108314585 A CN108314585 A CN 108314585A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
- C05D3/02—Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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Abstract
The invention discloses a kind of agriculture probiotics conversion stalks and the method for cultivating excrement production biological organic fertilizer, include the following steps：The pretreatment of stalk, the pretreatment for cultivating excrement, one time fermentation, pretreated straw and the pretreatment of pretreated straw and pretreatment cultivation excrement cultivate the secondary fermentation of excrement, obtain biological organic fertilizer.Agricultural crop straw and cultivation two kinds of raw material mixed fermentations of excrement are prepared biological organic fertilizer by the present invention, it can effectively avoid the limitation of single raw material, for agricultural crop straw or the cultivation single fermenting raw materials of excrement, faster, fermentation time is short, overall cost is lower for fermentation starting speed.In addition, biological organic fertilizer prepared by the present invention can dramatically increase the content of the nutrients such as the soil organism and phosphorus, potassium, quantity of useful microbe in nutrient conversion efficiency and product is improved, soil remediation, culture fertility are promoted, fertilizer application amount can be reduced, enhance the disease resistance of crop simultaneously, improve crop quality, reduces input, yield is improved, promotes to increase income.
Description
Technical field
The invention belongs to microbial fermentation technical field of fertilizers, and in particular to a kind of agricultural probiotics conversion stalk and cultivation
The method that excrement produces biological organic fertilizer.
Background technology
With the fast development of Chinese society economy, agricultural modernization level improves increasingly, various special raisings, plantation
Industry is surging forward throughout the country, not only enriches the market demand being growing, and has driven vast farmers income significantly
Horizontal raising.The farming and animal husbandries such as proportion of crop planting, beef cattle, milk cattle cultivating have formed relatively complete industry in drug in some provinces area
Structure, form from kind, support to postpartum preliminary working, product deep processing, high value added product extraction, both at home and abroad trade etc. one
A whole set of industrial system.
It is important to note, however, that rural areas in our country remains, energy resource structure is unreasonable, environmental issue is increasingly tight
The problems such as high, needs to give enough attention and solution.With the increase of crop acreage, the raising of planting technology, farming
Species are planted the byproduct such as maize straw, soybean stalk etc. that brings and are increasingly increased, since wood fibre cellulose content is higher, quality compared with
To be tough and tensile, moisture content is higher, is often discarded in field, dries burn-up, banks out or throw away on Around the house, canopy parting side
In dump pit, some is even thrown into river, and above-mentioned processing method can all cause the propagation of the pollution and pathogen to environment, point
Fire, which burns, then pollutes air, arbitrarily stacks and is then easy to grow pest and disease damage, becomes the important of the host, disease that pathogen depends on for existence
Propagating source adds stalk enormous amount, lacks effective quick processing mode；The animal wastes that livestock culture generates, although
Can directly apply fertilize the soil or through aerobic compost produce fertilizer, but there is also it is outdoor emit bad smell, multiply mosquitos and flies, pollution
The problems such as living environment.
Stalk and cultivation excrement anaerobic fermentation are produced into biological organic fertilizer using agriculture probiotics, not only can effectively be dissolved
The solid waste such as stalk, fowl and animal excrement, and efficient, green high-quality organic fertilizer can also be produced, there is very significant warp
Ji and environmental benefit.
Invention content
A kind of agriculture probiotics conversion stalk provided by the invention and the method for cultivating excrement production biological organic fertilizer, solve
Stalk enormous amount in the prior art lacks effective quick processing mode, although the animal wastes that livestock culture generates can
With directly application fertilize the soil or through aerobic compost produce fertilizer, but there are it is outdoor emit bad smell, multiply mosquitos and flies, pollution life
The problem of environment.
The present invention provides a kind of agriculture probiotics conversion stalks and the method for cultivating excrement production biological organic fertilizer, including
Following steps：
Step 1, stalk is collected after crop harvesting, stalk obtains stalk material residue after crushing；
Step 2, it is charged with the complex enzyme for being equivalent to cultivation stool quality 1% after cultivating collecting dung, is then existed
It is stirred under the mixing speed of 100-150r/min, and is passed through hot-air into cultivation excrement while agitating, when beginning is passed through
Temperature is 35-45 DEG C of hot-air 5-10h, and it is 50-55 DEG C of hot-air 3-5h to be subsequently passed temperature, is in being passed through temperature finally
75-80 DEG C of hot-air 2-3h, ventilation finish to obtain pretreatment cultivation excrement；
Wherein, the complex enzyme by protease, amylase and zytase according to 1：1：2 quality than mixed preparing and
At；
Step 3, the stalk material residue is uniformly mixed with pretreatment cultivation excrement, then adds water thereto, makes it
Water content reaches 50-60%, obtains preconditioned mixture；
By the preconditioned mixture and fermenting agent according to 200-300：It ferments at normal temperatures after 1 mass ratio mixing,
Fermentate temperature is detected in fermentation process, when fermentate temperature reaches 50 DEG C and fermentation time reaches 72h, is started for the first time
Fermentate turning forms new fermentation heap, and hole ventilation is then pricked on new fermentation heap, and duration of ventilation is for 24 hours；When fermentate
When temperature rises to 60 DEG C, turning again is carried out, and hereafter primary every 12-24h turnings, when fermentate temperature starts to reduce
Terminate fermentation, obtains one time fermentation product；
Wherein, the fermenting agent is by Achromobacter xylosoxidans zymocyte liquid, bacillusmusilaginosiengineering zymocyte liquid, rattan
Yellow micrococcus luteus zymocyte liquid, thermophilic streptomyces diastaticus zymotic fluid and cellulose-decomposing bacterium zymocyte liquid are according to 3：2：2：1：1
Mass ratio mix；
Effective total viable count is 1.0 × 10 in the fermenting agent8-1.0×1010cfu/mL；
Step 4, by one time fermentation product, calcium lime powder and function microbial inoculum according to 150-200：5-10：1 mass ratio mixing
After carry out secondary fermentation, secondary fermentation is completed after the temperature of secondary fermentation is 60-70 DEG C, 5-7 days, obtains the organic fertilizer that ferments；
Wherein, the function microbial inoculum by fixed nitrogen bacteria agent, phosphorus decomposing bacteria agent, potassium decomposing bacteria agent, Rhizobium Inoculant according to 25-
30：15-20：5-10：1 mass ratio mixes；And the fixed nitrogen bacteria agent, the phosphorus decomposing bacteria agent, the potassium solubilizing bacteria
In microbial inoculum, the Rhizobium Inoculant living bacteria count >=3.0 × 1010cfu/g；
Step 5, the fermentation organic fertilizer is dried, is granulated, is sieved, examines and is packed has to get to the biology
Machine fertilizer.
Preferably, the stalk is maize straw, soybean stalk, broomcorn straw or wheat stalk.
Preferably, the stalk material residue is the section shape of 3-5cm.
Preferably, the cultivation excrement is freshly-slaughtered poultry excrement, fresh pig dung, fresh cow dung, fresh sheep manure or fresh horsehit.
Preferably, the prolease activity, the amylase activity, the Xylanase activity are 6000-7000U/
mL。
Preferably, the nitrogen-fixing bacteria are azotobacter chroococcum or Azospirillum brasilense；The phosphate solubilizing bacteria is waxy Bacillus
Or bacillus badius；The potassium solubilizing bacteria is colloid bacillus cereus or bacillus megaterium；The rhizobium are stem tumor fixed nitrogen root
Tumor bacterium or magnificent last of the ten Heavenly stems rhizobium.
Green crushing is taken advantage of in the present invention, when stalk crushes, it is possible to reduce sugar loss in stalk, it is rotten to accelerating stalk
Solution increases soil nutrient and plays an important role；It cultivates and contains a large amount of crude protein, crude fibre, crude fat and nitrogen, phosphorus etc. in excrement,
General fermenting agent is less desirable to the degradation effect of crude protein, crude fat, and degradation speed is slow, therefore, in order to make to support
Grow excrement when with stalk mixed fermentation can efficient fast degradation, the present invention first to cultivation excrement pre-process.This hair
It is bright that cultivation excrement is pre-processed using complex enzyme, make crude protein therein, crude fat rapid enzyme under the action of complex enzyme
Solution forms the elements such as nitrogen, phosphorus；Meanwhile the present invention using complex enzyme to cultivation excrement pre-process when also in processing support
It grows excrement and has carried out hierarchical temperature air blast, on the one hand increase the cultivation intermolecular dissolved oxygen of excrement, improve enzymatic activity, it is another
The gas generated in enzymolysis process has been discharged in aspect, so that reaction is quickly carried out to the direction for generating product, to keep enzymolysis quick
It is efficient to carry out.
It is rich in a variety of nutrients in stalk, also contains various trace elements, can be carried to Soil Microorganism after everfermentation
For enough nutrients, moreover it is possible to which accumulation uses fermenting microbial inoculum to stalk first for the nutrient needed for plant growth, the present invention
It degrades, dominant microflora is on the one hand utilized to destroy the eucaryotic cell structure of stalk, effectively facilitate starch, protein, cellulose, wooden
The dissolution of the active ingredients such as element, to make the oxygenolysis such as most of cellulose, lignin difficult to degrade, with secondary hair after mitigation
The pressure of ferment；Carry out secondary fermentation immediately after one time fermentation, secondary fermentation when adds function microbial inoculum, function bacterium
On the one hand agent can make one time fermentation product further decomposed, Rapid Fermentation is at the organic fertilizer of high quality, another aspect, function bacterium
It is applied to soil after agent addition, can convert the invalid potassium not being absorbed by crops in soil and invalid phosphorus to can be absorbed by crops
Available potassium and rapid available phosphorus, while the multiple elements such as silicon in soil, manganese, zinc, molybdenum are discharged, improve trophic level；Phosphate solubilizing bacteria, potassium solubilizing bacteria
Generated in crop vital movement gibberellin, the basic element of cell division, the bioactive substances such as heteroauxin, can the life of effective stimulus crop
Long development；Nitrogen-fixing bacteria, rhizobium can from soil, air absorbed nitrogen, raise up seed, after death by remains " donations " give plant,
Plant is allowed to obtain a large amount of nitrogenous fertilizer.
The present invention adds calcium lime powder in secondary fermentation, and quick lime, which meets water, can generate calcium hydroxide, and it is anti-that micro- alkali occurs
It answers, releases a large amount of heat, the heat sent out promotes rapidly the temperature in fermentation process, accelerates decomposed speed, while consuming, evaporating
Partial moisture reduces tunning moisture；Calcium hydroxide can neutralize a large amount of acetic acid generated in fermentation process simultaneously, make most
Between the pH value of the organic fertilizer obtained eventually maintains 6-8, it will not cause to apply rear soil to be in highly acid, in addition, in organic fertilizer containing
There is moderate-element calcium, there is no need to additional supplementing calcium elements using the plant of the organic fertilizer.
The above 5 class strain complements one another, and with 4 kinds of function strain mating reactions, so that raw material is finally fermented into good, agriculture
The organic fertilizer that crop easily absorbs.
Compared with prior art, the beneficial effects of the present invention are：
1) agricultural crop straw and cultivation two kinds of raw material mixed fermentations of excrement are prepared biological organic fertilizer by the present invention, can effectively be kept away
Exempt from the limitation of single raw material, compared to agricultural crop straw or cultivation the single fermenting raw materials of excrement for, fermentation starting speed faster,
Fermentation time is short, course of fermentation is more stable, overall cost is lower.
2) biological organic fertilizer that the present invention prepares can dramatically increase containing for the nutrients such as the soil organism and phosphorus, potassium
Amount improves quantity of useful microbe in nutrient conversion efficiency and product, promotes soil remediation, culture fertility that can reduce fertilizer application
Amount, while enhancing the disease resistance of crop, improve crop quality, reduce input, improves yield, promote to increase income.
Specific implementation mode
In order to enable those skilled in the art to more fully understand, technical scheme of the present invention is practiced, with reference to specific
The invention will be further described for embodiment, but illustrated embodiment is not as a limitation of the invention.
Achromobacter xylosoxidans used in the present invention (Achromobacterxylosoxidans) are China Microbiological
Culture presevation administration committee common micro-organisms center deposit number is the bacterial strain of CGMCC No.8512；Bacillus mucilaginosus
(Bacillus mucilaginosus) is that Chinese agriculture Microbiological Culture Collection administrative center deposit number is ACCC 10013
Bacterial strain；Micrococcus luteus (Micrococcusluteus) is China Committee for Culture Collection of Microorganisms's common micro-organisms
Center deposit number is the bacterial strain of CGMCC No.6072；Thermophilic streptomyces diastaticus (Streptomyces
Thermodiastaticus be China Committee for Culture Collection of Microorganisms's common micro-organisms center's deposit number it is) CGMCC
The bacterial strain of No.12134；Cellulose-decomposing bacterium (Thermoanaerobacterium sp.L-D) is Chinese agriculture microorganism fungus kind
Preservation administrative center deposit number is CCTCC NO：The bacterial strain of M208166.
Achromobacter xylosoxidans zymocyte liquid, Bacillus mucilaginosus zymocyte liquid, micrococcus luteus hair in embodiment
Yeast-like fungi liquid, thermophilic streptomyces diastaticus zymocyte liquid, cellulose-decomposing bacterium zymocyte liquid are obtained using conventional method culture
's；Fixed nitrogen bacteria agent used, phosphorus decomposing bacteria agent, potassium decomposing bacteria agent, Rhizobium Inoculant purchase are in Borrow Di Muhan (Weifang) biologies
Science and Technology Ltd., experimental method described in following each embodiments are unless otherwise specified conventional method.
Embodiment 1
A kind of method of agricultural probiotics conversion stalk and cultivation excrement production biological organic fertilizer, specifically in accordance with the following steps
It prepares：
Step 1, stalk is collected after crop harvesting, stalk obtains stalk material residue after being ground into the section shape of 3cm；
Step 2, it is charged with the complex enzyme for being equivalent to cultivation stool quality 1% after cultivating collecting dung, is then existed
It is stirred under the mixing speed of 100r/min, and is passed through hot-air into cultivation excrement while agitating, when beginning is passed through temperature
For 40 DEG C of hot-air 8h, it is subsequently passed the hot-air 5h that temperature is 50 DEG C, is finally passed through the hot-air 3h that temperature is 75 DEG C, is led to
Wind finishes to obtain pretreatment cultivation excrement；
Wherein, complex enzyme by protease, amylase and zytase according to 1：1：2 quality is formed than mixed preparing；And
Prolease activity is 6000U/mL, amylase activity 6500U/mL, Xylanase activity are 7000U/mL；
Step 3, stalk material residue is uniformly mixed with pretreatment cultivation excrement, then adds water thereto, its water content is made to reach
To 50%, preconditioned mixture is obtained；
By preconditioned mixture and fermenting agent according to 200：It ferments at normal temperatures after 1 mass ratio mixing, fermentation process
Middle detection fermentate temperature starts first time fermentate and turns over when fermentate temperature reaches 50 DEG C, and fermentation time reaches 72h
Heap forms new fermentation heap, and hole ventilation is then pricked on new fermentation heap, and duration of ventilation is for 24 hours；When the temperature of fermentate rises to
At 60 DEG C, turning again is carried out, and hereafter primary every turning for 24 hours, terminates to ferment until fermentate temperature starts the when of reducing, obtain
To one time fermentation product；
Wherein, fermenting agent is micro- by Achromobacter xylosoxidans zymocyte liquid, bacillusmusilaginosiengineering zymocyte liquid, gamboge
Coccus zymocyte liquid, thermophilic streptomyces diastaticus zymotic fluid and cellulose-decomposing bacterium zymocyte liquid are according to 3：2：2：1：1 matter
Amount ratio mixes；
Effective total viable count is 1.0 × 10 in fermenting agent8cfu/mL；
Step 4, by one time fermentation product, calcium lime powder and function microbial inoculum according to 180：8：It is carried out after 1 mass ratio mixing
Secondary fermentation, secondary fermentation is completed after the temperature of secondary fermentation is 60 DEG C, 7 days, obtains fermentation organic fertilizer；
Wherein, the function microbial inoculum by fixed nitrogen bacteria agent, phosphorus decomposing bacteria agent, potassium decomposing bacteria agent, Rhizobium Inoculant according to 28：
18：7：1 mass ratio mixes；And in fixed nitrogen bacteria agent, phosphorus decomposing bacteria agent, potassium decomposing bacteria agent, Rhizobium Inoculant effectively
Viable count is >=3.0 × 1010cfu/g；
Step 5, fermentation organic fertilizer is dried, be granulated, be sieved, examines and packed to get to biological organic fertilizer.
Embodiment 2
A kind of method of agricultural probiotics conversion stalk and cultivation excrement production biological organic fertilizer, specifically in accordance with the following steps
It prepares：
Step 1, stalk is collected after crop harvesting, stalk obtains stalk material residue after being ground into the section shape of 4cm；
Step 2, it is charged with the complex enzyme for being equivalent to cultivation stool quality 1% after cultivating collecting dung, is then existed
It is stirred under the mixing speed of 120r/min, and is passed through hot-air into cultivation excrement while agitating, when beginning is passed through temperature
For 45 DEG C of hot-air 5h, it is subsequently passed the hot-air 4h that temperature is 53 DEG C, is finally passed through the hot-air 2h that temperature is 80 DEG C, is led to
Wind finishes to obtain pretreatment cultivation excrement；
Wherein, complex enzyme by protease, amylase and zytase according to 1：1：2 quality is formed than mixed preparing；And
Prolease activity is 7000U/mL, amylase activity 6500U/mL, Xylanase activity are 6000U/mL；
Step 3, stalk material residue is uniformly mixed with pretreatment cultivation excrement, then adds water thereto, its water content is made to reach
To 55%, preconditioned mixture is obtained；
By preconditioned mixture and fermenting agent according to 250：It ferments at normal temperatures after 1 mass ratio mixing, fermentation process
Middle detection fermentate temperature starts first time fermentate and turns over when fermentate temperature reaches 50 DEG C, and fermentation time reaches 72h
Heap forms new fermentation heap, and hole ventilation is then pricked on new fermentation heap, and duration of ventilation is for 24 hours；When the temperature of fermentate rises to
At 60 DEG C, turning again is carried out, and hereafter primary every 18h turnings, terminates to ferment until fermentate temperature starts the when of reducing, obtain
To one time fermentation product；
Wherein, fermenting agent is micro- by Achromobacter xylosoxidans zymocyte liquid, bacillusmusilaginosiengineering zymocyte liquid, gamboge
Coccus zymocyte liquid, thermophilic streptomyces diastaticus zymotic fluid and cellulose-decomposing bacterium zymocyte liquid are according to 3：2：2：1：1 matter
Amount ratio mixes；
Effective total viable count is 1.0 × 10 in fermenting agent9cfu/mL；
Step 4, by one time fermentation product, calcium lime powder and function microbial inoculum according to 200：5：It is carried out after 1 mass ratio mixing
Secondary fermentation, secondary fermentation is completed after the temperature of secondary fermentation is 65 DEG C, 6 days, obtains fermentation organic fertilizer；
Wherein, the function microbial inoculum by fixed nitrogen bacteria agent, phosphorus decomposing bacteria agent, potassium decomposing bacteria agent, Rhizobium Inoculant according to 25：
15：10：1 mass ratio mixes；And in fixed nitrogen bacteria agent, phosphorus decomposing bacteria agent, potassium decomposing bacteria agent, Rhizobium Inoculant effectively
Viable count is >=3.0 × 1010cfu/g；
Step 5, fermentation organic fertilizer is dried, be granulated, be sieved, examines and packed to get to biological organic fertilizer.
Embodiment 3
A kind of method of agricultural probiotics conversion stalk and cultivation excrement production biological organic fertilizer, specifically in accordance with the following steps
It prepares：
Step 1, stalk is collected after crop harvesting, stalk obtains stalk material residue after being ground into the section shape of 5cm；
Step 2, it is charged with the complex enzyme for being equivalent to cultivation stool quality 1% after cultivating collecting dung, is then existed
It is stirred under the mixing speed of 150r/min, and is passed through hot-air into cultivation excrement while agitating, when beginning is passed through temperature
For 35 DEG C of hot-air 10h, it is subsequently passed the hot-air 3h that temperature is 55 DEG C, is finally passed through the hot-air that temperature is 78 DEG C
2.5h, ventilation finish to obtain pretreatment cultivation excrement；
Wherein, complex enzyme by protease, amylase and zytase according to 1：1：2 quality is formed than mixed preparing；And
Prolease activity is 6500U/mL, amylase activity 7000U/mL, Xylanase activity are 7000U/mL；
Step 3, stalk material residue is uniformly mixed with pretreatment cultivation excrement, then adds water thereto, its water content is made to reach
To 60%, preconditioned mixture is obtained；
By preconditioned mixture and fermenting agent according to 300：It ferments at normal temperatures after 1 mass ratio mixing, fermentation process
Middle detection fermentate temperature starts first time fermentate and turns over when fermentate temperature reaches 50 DEG C, and fermentation time reaches 72h
Heap forms new fermentation heap, and hole ventilation is then pricked on new fermentation heap, and duration of ventilation is for 24 hours；When the temperature of fermentate rises to
At 60 DEG C, turning again is carried out, and hereafter primary every 12h turnings, terminates to ferment until fermentate temperature starts the when of reducing, obtain
To one time fermentation product；
Wherein, fermenting agent is micro- by Achromobacter xylosoxidans zymocyte liquid, bacillusmusilaginosiengineering zymocyte liquid, gamboge
Coccus zymocyte liquid, thermophilic streptomyces diastaticus zymotic fluid and cellulose-decomposing bacterium zymocyte liquid are according to 3：2：2：1：1 matter
Amount ratio mixes；
Effective total viable count is 1.0 × 10 in fermenting agent10cfu/mL；
Step 4, by one time fermentation product, calcium lime powder and function microbial inoculum according to 150：10：It is carried out after 1 mass ratio mixing
Secondary fermentation, secondary fermentation is completed after the temperature of secondary fermentation is 70 DEG C, 5 days, obtains fermentation organic fertilizer；
Wherein, the function microbial inoculum by fixed nitrogen bacteria agent, phosphorus decomposing bacteria agent, potassium decomposing bacteria agent, Rhizobium Inoculant according to 30：
20：5：1 mass ratio mixes；And in fixed nitrogen bacteria agent, phosphorus decomposing bacteria agent, potassium decomposing bacteria agent, Rhizobium Inoculant effectively
Viable count is >=3.0 × 1010cfu/g；
Step 5, fermentation organic fertilizer is dried, be granulated, be sieved, examines and packed to get to biological organic fertilizer.
It should be noted that agricultural crop straw is maize straw, soybean stalk, broomcorn straw or wheat stalk；Cultivate excrement
Just it is freshly-slaughtered poultry excrement, fresh pig dung, fresh cow dung, fresh sheep manure or fresh horsehit.
The biological organic fertilizer of embodiment 1-3 can improve the physicochemical property of soil, the life for below preparing the present invention
Organic fertilizer is applied to wheat planting to illustrate the effect of the present invention.
Experimental subjects：40 mu of improving yield of wheat nonirrigated farmlands are divided into four groups, and every group 10 mu, wherein three groups are test group, this is not applied
The biological organic fertilizer that embodiment 1-3 is prepared；Another set is control group, using common fertilizer.
Experimental method：It in 6 months, is tested under the conditions of same noiseless, such as 40 mu of ground nutrition phases
It is approximate or identical, wheat of the same area is planted in same season, is carried out at the same time fertilising, and the biology that embodiment 1-3 is prepared
Organic fertilizer is applied as base manure, and control group applies common farm manure as base manure, and applied amount is 800kg/ mus.
Topsoil soils before acquisition applies respectively in experimentation and after harvesting wheat, analyze its basic physics and chemistry number
Value, concrete outcome are shown in Table 1.
Table 1 applies front and back soil physico-chemical property
As it can be seen from table 1 on improving yield of wheat dryland soil after the biological organic fertilizer of embodiment 1-3, the physics and chemistry of soil
Property has obtained prodigious change, and not only the content of Nutrient Elements in Soil increases significantly, and fertility greatly improves, but also reduces
The soil weight, increases soil porosity.In general, in agricultural crop straw and cultivation excrement the nitrogen P elements that contain compared with
It is low, and nitrogen P elements are also strong by soil environment (air, temperature, moisture, microbial activities) and crop vigor and breathing
The influence of degree, content can further decrease, and in order to maintain plant growth, generally require and in addition apply composite fertilizer, and embodiment 1-3
Function microbial inoculum is just supplemented when secondary fermentation, is not only provided enough N to reduce C/N in the secondary fermentation stage, is tieed up
Microorganism normal growth is held, and remaining a large amount of nitrogen, phosphorus, potassium element can be also retained in the biological organic fertilizer that fermentation obtains,
It disclosure satisfy that the needs of edaphon and plant growth.
Start to sow wheat after using the biological organic fertilizer of embodiment 1-3 on improving yield of wheat nonirrigated farmland, while general with application
Wheat is sowed in the improving yield of wheat nonirrigated farmland of logical farm manure as a comparison, and applied amount is 800kg/ mus, in the process of wheat growth
In no longer apply other fertilizer, its yield is recorded after harvesting wheat, concrete outcome is shown in Table 2.
2 wheat yield of table
| Project | Wheat yield (kg/hm2) |
| Control group | 5625.5 |
| Embodiment 1 | 6486.6 |
| Embodiment 2 | 6452.8 |
| Embodiment 3 | 6518.3 |
From table 2 it can be seen that after using the biological organic fertilizer plantation wheat of embodiment 1, relative to using common farm manure
For the wheat of plantation, the output increased of wheat 15.31%；After the biological organic fertilizer plantation wheat of embodiment 2, relatively
For the wheat of the common farm nanure planting of application, the output increased of wheat 14.71%, using the biological organic of embodiment 3
After fertilizer plantation wheat, for the wheat of the common farm nanure planting of application, the output increased of wheat 15.87%；Thus
As it can be seen that the biological organic fertilizer of the present invention can improve soil physico-chemical property, increase soil fertility, and then improves crop yield.
It should be noted that involved in claims of the present invention when numberical range, it is thus understood that each numberical range
Any one numerical value can be selected between two endpoints and two endpoints, due to step method and the embodiment 1-3 phases of use
Together, it repeats in order to prevent, description of the invention preferred embodiment, once a person skilled in the art knows basic
Creative concept, then additional changes and modifications may be made to these embodiments.It is wrapped so the following claims are intended to be interpreted as
It includes preferred embodiment and falls into all change and modification of the scope of the invention.
Obviously, various changes and modifications can be made to the invention without departing from essence of the invention by those skilled in the art
God and range.In this way, if these modifications and changes of the present invention belongs to the range of the claims in the present invention and its equivalent technologies
Within, then the present invention is also intended to include these modifications and variations.
Claims (5)
1. a kind of agricultural probiotics conversion stalk and the method for cultivating excrement production biological organic fertilizer, which is characterized in that specifically press
Implement according to following steps：
Step 1, stalk is collected after crop harvesting, stalk obtains stalk material residue after crushing；
Step 2, it is charged with the complex enzyme for being equivalent to cultivation stool quality 1% after cultivating collecting dung, then in 100-
It is stirred under the mixing speed of 150r/min, and is passed through hot-air into cultivation excrement while agitating, when beginning is passed through temperature
For 35-45 DEG C of hot-air 5-10h, it is subsequently passed the hot-air 3-5h that temperature is 50-55 DEG C, is finally 75-80 in being passed through temperature
DEG C hot-air 2-3h, ventilation finish to obtain pretreatment cultivation excrement；
Wherein, the complex enzyme by protease, amylase and zytase according to 1：1：2 quality is formed than mixed preparing；
Step 3, the stalk material residue is uniformly mixed with pretreatment cultivation excrement, then adds water thereto, keeps its aqueous
Amount reaches 50-60%, obtains preconditioned mixture；
By the preconditioned mixture and fermenting agent according to 200-300：It ferments, ferments at normal temperatures after 1 mass ratio mixing
Fermentate temperature is detected in the process, when fermentate temperature reaches 50 DEG C and fermentation time reaches 72h, by first time fermentate
Turning forms new fermentation heap, and hole ventilation is then pricked on new fermentation heap, and duration of ventilation is for 24 hours；When fermentate temperature rises to
At 60 DEG C, turning again is carried out, and hereafter primary every 12-24h turnings, terminates to send out until fermentate temperature starts the when of reducing
Ferment obtains one time fermentation product；
Wherein, the fermenting agent is micro- by Achromobacter xylosoxidans zymocyte liquid, bacillusmusilaginosiengineering zymocyte liquid, gamboge
Coccus zymocyte liquid, thermophilic streptomyces diastaticus zymotic fluid and cellulose-decomposing bacterium zymocyte liquid are according to 3：2：2：1：1 matter
Amount ratio mixes；
Effective total viable count is 1.0 × 10 in the fermenting agent8-1.0×1010cfu/mL；
Step 4, by one time fermentation product, calcium lime powder and function microbial inoculum according to 150-200：5-10：1 mass ratio mixing is laggard
Row secondary fermentation, secondary fermentation is completed after the temperature of secondary fermentation is 60-70 DEG C, 5-7 days, obtains the organic fertilizer that ferments；
Wherein, the function microbial inoculum by fixed nitrogen bacteria agent, phosphorus decomposing bacteria agent, potassium decomposing bacteria agent, Rhizobium Inoculant according to 25-30：
15-20：5-10：1 mass ratio mixes；And the fixed nitrogen bacteria agent, the phosphorus decomposing bacteria agent, the potassium solubilizing bacteria bacterium
In agent, the Rhizobium Inoculant living bacteria count >=3.0 × 1010cfu/g；
Step 5, the fermentation organic fertilizer is dried, be granulated, be sieved, examines and packed to get to the biological organic fertilizer.
2. agricultural probiotics conversion stalk according to claim 1 and the method for cultivating excrement production biological organic fertilizer,
It is characterized in that, the stalk is maize straw, soybean stalk, broomcorn straw or wheat stalk.
3. agricultural probiotics conversion stalk according to claim 2 and the method for cultivating excrement production biological organic fertilizer,
It is characterized in that, the stalk material residue is the section shape of 3-5cm.
4. agricultural probiotics conversion stalk according to claim 1 and the method for cultivating excrement production biological organic fertilizer,
It is characterized in that, the cultivation excrement is freshly-slaughtered poultry excrement, fresh pig dung, fresh cow dung, fresh sheep manure or fresh horsehit.
5. agricultural probiotics conversion stalk according to claim 1 and the method for cultivating excrement production biological organic fertilizer,
It is characterized in that, the prolease activity, the amylase activity, the Xylanase activity are 6000-7000U/mL.
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