CN106631551A - Plant-growth-promoting bio-organic fertilizer and preparation method - Google Patents
Plant-growth-promoting bio-organic fertilizer and preparation method Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C9/00—Fertilisers containing urea or urea compounds
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
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Abstract
Plant-growth-promoting bio-organic fertilizer is prepared by inoculating 0.5-2% (w/w) of composite fermentation microbial agent to livestock-poultry manure and auxiliary material, fermenting, inoculating 2-5% (w/w) of plant-growth-promoting microbial agent and fermenting. A preparation method includes steps: (1), preparing a fermenting material; (2), performing high-temperature aerobic fermentation; (3), preparing the plant-growth-promoting microbial agent; (4), after-composting organic fertilizer. The plant-growth-promoting bio-organic fertilizer and the preparation method have the advantages that rapid proliferation of plant-growth-promoting functional microorganisms is realized in the process of fermenting of two times, and effective biological viable bacteria count of the organic fertilizer reaches 5x108CFU/g; composting degree is high, the organic fertilizer can generate various plant hormones including 3-indoleacetic acid, cytokinin, gibberellins and ethylene after being applied in soil, can promote plant growth and can increase yield of crops and incoming of farmers; the bio-organic fertilizer prepared by the method is low in cost, environment-friendly and high in efficiency.
Description
Technical field
The invention belongs to technical field of biological organic fertilizer, and in particular to one kind produces plant growth-promoting using livestock manure fermented
Type biological organic fertilizer and its method.
Background technology
With scale, the fast development of intensive livestock and poultry breeding industry, feces of livestock and poultry is used as the main of widespread pollution from the overuse of fertilizers and pesticides in rural area
Source has become the outstanding problem of environmental pollution.On the other hand, in Pollution from livestock and poultry thing containing abundant organic matter, nitrogen, phosphorus,
The nutrient substance such as potassium and trace element, with great resource potentiality.It is current poultry using aerobic fermentation production fertilizer
Night soil-treatment and the topmost means of recycling.However, there is fermentation in the technique of traditional feces of livestock and poultry production fertilizer
The nutritive losses such as cycle length, nitrogen are serious, the low various problems of organic fertilizer product fertilizer efficiency, therefore, develop novel microorganism work(
Energy microbial inoculum has become the development trend in the field as organic fertilizer additive.
Biological organic fertilizer refer to specified microorganisms and plant and animal residues as source and Jing harmless treatments, become thoroughly decomposed it is organic
What material was composited has the fertilizer of microbial manure and fertilizer fertilizer efficiency concurrently.Standard biologic organic fertilizer production technique is to ferment
Bacterium and function bacterium co-fermentation, but because function bacterium is generally adapted to the environment such as soil, plant rhizosphere, it is difficult in aerobic fermentation
Hot stage is survived, and strongly limit the activity of functional microorganism in fertilizer.At present, based on two-part fermentation technology step by step,
It is generally satisfactory by way of adding corresponding function microbial bacterial agent in the different fermentations stage.Chinese patent document CN
102964155 B disclose a kind of preparation method of interior azotobacter fertilizer, are just inoculated with by hay using the first fermentation stage
Bacillus cereuss, Bacillus licheniformis, beer yeast, button capsule intend endosporaceae, Trichoderma viride, sporotrichum thermophile, Lactobacillus plantarum, black
The compound microorganism ferments microbial inoculum of aspergillosis composition, and be inoculated with by pantoea agglomerans, bacillus megaterium, colloid in the ferment in second time stage
Growing nitrogen-fixing microbial inoculum in the complex microorganism of bacillus cereuss composition, so as to prepare the fertilizer with fixed nitrogen function.Even so, existing
Have bio-organic fertilizer product still there are problems that product category it is few,.
The content of the invention
It is an object of the invention to provide a kind of plant growth-promoting type biological organic fertilizer and preparation method thereof, aerobic by combining
Fermentation and the mode of addition functional microorganism microbial inoculum, produce the biological organic fertilizer type with plant growth-promoting function, are substituting
Improved soil microorganism ecological environment while chemical fertilizer products, promotes plant growing.
For achieving the above object, the present invention is employed the following technical solutions：
A kind of plant growth-promoting type biological organic fertilizer, by animal dung, adjuvant the composite fermentation microbial inoculum of 0.5～2% (w/w) is inoculated with
The plant growth-promoting microbial inoculum fermentation that 2～5% (w/w) are inoculated after fermentation is made, and the adjuvant is straw, rice husk, mushroom residue, wheat
At least one in bran, carbamide, it is characterised in that：The composite fermentation microbial inoculum is by Methylotrophic bacillus cereuss (Bacillus
Methylotrophicus KACC 13105), Te Jila bacillus cereuss (Bacillus tequilensis ATCC BAA-
819), Geobacillus stearothermophilus (Gebacillus stearothermophilus ATCC 12980) and yellow is thermophilic detests
Oxygen bacillus cereuss Yunnan subspecies (Anoxybacillus flavithermus ssp.yunnanesis CCTCC AB2010187)
Mix according to mass ratio 0.5～1: 1～2: 0.5～1: 1；The plant growth-promoting microbial inoculum is by liquid humid acid fertilizer azospirillum
(Azospirillum humicireducens CCTCC AB 2012021), Candida utilis (Candida utilis
CCTCC M 2010090), streptomyces microflavus (Streptomyces microflavus CGMCC 4.891) and fixed nitrogen class spore
Bacillus (Paenibacillus azotofixans ATCC35681) in mass ratio 1～2: 1～2: 1～2: 1 ratio mixing and
Into.
A kind of preparation method of plant growth-promoting type biological organic fertilizer, it is characterised in that：Comprise the following steps that：
(1) prepared by fermentation material：With at least in straw, rice husk, mushroom residue, Testa Tritici, the carbamide after animal dung and crushing
Mixing is planted, moisture and C/N is adjusted respectively to 55%～70% and 25～35, fermentation material is obtained；
(2) high-temperature aerobic fermentation：The composite fermentation microbial inoculum of 0.5～2% (w/w) is inoculated with step (1) fermentation materials, is mixed
Be mixed even laggard aerobe fermentation of acting charitably；Turning is once, afterwards per 2～3d turnings once, continuous to send out after heap temperature is more than 60 DEG C
15～20d of ferment obtains first fermentation product；
The composite fermentation microbial inoculum is by Methylotrophic bacillus cereuss (Bacillus methylotrophicus KACC
13105), Te Jila bacillus cereuss (Bacillus tequilensis ATCC BAA-819), Geobacillus stearothermophilus
(Gebacillus stearothermophilus ATCC 12980) and yellow thermophilic anaerobic bacillus cereuss Yunnan subspecies
(Anoxybacillus flavithermus ssp.yunnanesis CCTCC AB2010187) is according to mass ratio 0.5～1: 1
Mix at～2: 0.5～1: 1；
(3) prepared by plant growth-promoting microbial inoculum：By the liquid humid acid fertilizer azospirillum (Azospirillum being preserved on inclined-plane
Humicireducens CCTCC AB 2012021), Candida utilis (Candida utilis CCTCC M
2010090), streptomyces microflavus (Streptomyces microflavus CGMCC 4.891) and fixed nitrogen series bacillus
(Paenibacillus azotofixans ATCC 35681) is inoculated into respectively LB fluid mediums, under conditions of 28～32 DEG C
24～48h of shaking table culture；By the ratio mix homogeneously of the bacterium solution in mass ratio 1～2: 1～2: 1～2: 1 of above-mentioned 4 kinds of bacterium, must plant
Thing growth-promoting composite bacteria agent capable.
The LB culture medium prescriptions are：Tryptone 10g/L, yeast extract 5g/L, NaCl 10g/L.
(4) become thoroughly decomposed after fertilizer：With step (2) prepare first fermentation product as raw material, according to the ratio of 2～5% (w/w)
Plant growth-promoting composite bacteria agent capable prepared by example addition step (3), 10～15d of standing for fermentation (period turning is once), you can be obtained and plant
Thing growth-promoting type bio-organic fertilizer product.
The invention has the beneficial effects as follows：
The present invention is directed to feces of livestock and poultry two-part aerobic fermentation process flow process, is prepared for being respectively used to hot stage and becomes thoroughly decomposed
Two kinds of complex microbial inoculums in stage：Composite fermentation microbial inoculum and plant growth-promoting composite bacteria agent capable；Both can use cooperatively, and also may be used
It is used alone.Wherein, composite fermentation microbial inoculum is formed by the 4 kinds of functional microorganisms compounding being isolated from During High-Temperature Composting system, is added
After can be with indigenous compost microbe synergism, the organic matter in fast decoupled feces of livestock and poultry, programming rate is fast, fermentation 15d
It is capable of achieving compost to become thoroughly decomposed completely, significantly improves the fermentation efficiency and stable degree of feces of livestock and poultry.Plant growth-promoting composite bacteria agent capable
The functional microorganism for being equally based on 4 kinds of high yield phytohormone is formed by lot of experiments compounding, with middle low temperature liquid fermentation mode
Spread cultivation, and with the organic substrates after feces of livestock and poultry first fermentation as raw material, plant growth-promoting type is realized during ferment in second time
The fast breeding of functional microorganism, its effective biologic live bacteria is total up to 5 × 108CFU/g, is far above《Biological organic fertilizer》(NY/T
798-2004) in standard >=0.2 × 108CFU/g numerical value.The bio-organic fertilizer product prepared based on invention degree of becoming thoroughly decomposed is high,
After being applied to soil, the functional microorganism of substantial amounts after rhizosphere colonization etc., can produce 3-indolyl acetic acid, the basic element of cell division,
Gibberellins, ethylene etc. promote plant strain growth in interior various plants hormone, and increase income crop yield.Life prepared by the invention
The features such as organic fertilizer has low cost, environment-friendly high-efficiency concurrently.
Specific embodiment
The present invention is further described with reference to specific embodiment.
Embodiment 1～4
Biological organic fertilizer is prepared with pig manure, corn straw, carbamide and functional microorganism microbial inoculum mixed fermentation as follows：
(1) biological organic fertilizer is prepared by fermenting raw materials of pig manure, corn straw and carbamide, fermentation materials gross mass is
300kg, according to pig manure: corn straw: the weight in wet base of carbamide 5: 1: 0.02 is shown in Table 1 than dispensing, three's material propertiess.Mixed thing
Material C/N ratios are 30.4, and moisture is 64.7%.
The pig manure of table 1, corn straw and carbamide material propertiess
| Project | Pig manure | Corn straw | Carbamide |
| Total organic carbon (gkg-1) | 374.5 | 441.8 | 0 |
| Total nitrogen (gkg-1) | 11.4 | 5.7 | 46.7 |
| C/N | 32.9 | 77.5 | 0 |
| Moisture (%) | 73.8 | 20.4 | 11.5 |
(2) by the Methylotrophic bacillus cereuss of slant preservation, Te Jila bacillus cereuss, Geobacillus stearothermophilus and
Yellow thermophilic anaerobic bacillus cereuss Yunnan subspecies respectively take 2 rings and are inoculated in LB culture fluid, respectively at 30 DEG C, 45 DEG C, 55 DEG C and 60
24h is activated at DEG C.Afterwards culture fluid is seeded to into amplification culture in new LB culture medium by 10% inoculum concentration.Fermentation ends
Afterwards, turf absorption microbial inoculum is added with 2: 1 mass ratio.By Methylotrophic bacillus cereuss for obtaining, Te Jila bacillus cereuss, thermophilic
Hot fat ground bacillus and 4 kinds of microbial inoculums of yellow thermophilic anaerobic bacillus cereuss Yunnan subspecies are mixed by 0.5～1: 1～2: 0.5～1: 1
Composite fermentation microbial inoculum is made in conjunction.
Inoculum concentration composite fermentation microbial inoculum according to 0.5～2% (w/w) adds in the compost material got ready to step (1), mixes
Be mixed it is even after start aerobic fermentation.Heap temperature more than 60 DEG C after turning once, afterwards per 2～3d turnings once, continuous fermentation
15～20d obtains first fermentation product.
(3) by the liquid humid acid fertilizer azospirillum of slant preservation, Candida utilis, streptomyces microflavus and fixed nitrogen class spore
Bacillus is inoculated into respectively LB fluid mediums, 30 DEG C of shaking table cultures 48h.By the bacterium solution in mass ratio 1～2: 1～2 of above-mentioned 4 kinds of bacterium
: 1～2: 1 uniform mixing, obtain plant growth-promoting composite bacteria agent capable.With first fermentation product as raw material, according to the ratio of 2～5% (w/w)
Example addition plant growth-promoting composite bacteria agent capable, 10～15d of standing for fermentation (period turning is once), you can plant growth-promoting type biology is obtained to be had
Machine fertilizer product.
The technique that bio-organic fertilizer product is prepared in embodiment 1～4 is as shown in table 2 with the index similarities and differences.
Comparative example 1
It is as follows organic fertilizer product to be prepared using pig manure, corn straw and urea fermentation：
According to the mixed material for preparing pig manure, corn straw and carbamide with the identical mode of embodiment 1～4, without
Natural fermentation under conditions of composite fermentation microbial inoculum.Ferment the 3rd day, heap temperature rises to 61 DEG C, start turning, turn over per 3d afterwards
Heap once, after continuous fermentation 18d obtains first fermentation product.First fermentation product is continued into composting, plant rush is not equally inoculated with
Raw composite bacteria agent capable carries out ferment in second time.Ferment in second time continues 15d, and in 7d, once, fermenting twice complete cycle is for turning
33d.After fermentation ends, according to《Biological organic fertilizer》The property of (NY/T 798-2004) standard detection composting production, and measure
The seed germination index of ferment product, the results are shown in Table 2.
The embodiment 1～4 of table 2 prepares the biological organic fertilizer scheme similarities and differences with comparative example 1
According to the result of embodiment 1～4, two in the present invention are adopted for raw material using pig manure, corn straw and carbamide
The segmentation bio-organic fertilizer product that step by step prepared by fermentation technology is met in NY/T 798-2004 about the requirement of standard.By
The composite fermentation microbial inoculum and plant growth-promoting composite bacteria agent capable of different ratio and quantity significantly increases in the different fermentations stage addition present invention
The living bacteria count in bio-organic fertilizer product is added.The testing result of seed germination index (GI) also indicates that, embodiment 1～4
The middle bio-organic fertilizer product prepared using the present invention is higher compared with the degree of becoming thoroughly decomposed of comparative example 1, good to plant growth-promoting effect.
Growth-promoting effect of the biological organic fertilizer to plant is further illustrated below based on embodiment 1 and comparative example 1.
Biological organic fertilizer prepared by embodiment 1 and comparative example 1 is applied in rice terrace according to the amount of every mu of 200kg, is replaced
40% chemical fertilizer applied amount of generation.Survey result points out that the biological organic fertilizer in applying embodiment 1 is compared with the fertilizer for applying comparative example 1
Oryza sativa L. is set to increase income 27%.
Embodiment 5～8
Biological organic fertilizer is prepared with chicken manure, rice husk and functional microorganism microbial inoculum mixed fermentation：
(1) biological organic fertilizer is prepared as fermenting raw materials with chicken manure and the rice husk crushed, fermentation materials gross mass is 200kg,
According to chicken manure, rice husk 5: 3 weight in wet base than dispensing, both are shown in Table 3 at material propertiess.Mixed material C/N ratios are 27, moisture
For 44.8%.Addition moisture adjusts heap body moisture content to 60% before fermentation starts.
The material propertiess of the chicken manure of table 3 and rice husk
| Project | Chicken manure | Rice husk |
| Total organic carbon (gkg-1) | 305 | 457.2 |
| Total nitrogen (gkg-1) | 30.6 | 4.1 |
| C/N | 10 | 111.5 |
| Moisture (%) | 64.8 | 11.8 |
(2) by the Methylotrophic bacillus cereuss of slant preservation, Te Jila bacillus cereuss, Geobacillus stearothermophilus and
Yellow thermophilic anaerobic bacillus cereuss Yunnan subspecies respectively take 2 rings and are inoculated in LB culture fluid, respectively at 30 DEG C, 45 DEG C, 55 DEG C and 60
24h is activated at DEG C.Afterwards culture fluid is seeded to into amplification culture in new LB culture medium by 10% inoculum concentration.Fermentation ends
Afterwards, turf absorption microbial inoculum is added with 2: 1 mass ratio.By Methylotrophic bacillus cereuss for obtaining, Te Jila bacillus cereuss, thermophilic
Hot fat ground bacillus and 4 kinds of microbial inoculums of yellow thermophilic anaerobic bacillus cereuss Yunnan subspecies are mixed by 0.5～1: 1～2: 0.5～1: 1
Composite fermentation microbial inoculum is made in conjunction.
Inoculum concentration composite fermentation microbial inoculum according to 0.5～2% (w/w) adds in the compost material got ready to step (1), mixes
Be mixed it is even after start aerobic fermentation.Heap temperature more than 60 DEG C after turning once, afterwards per 2～3d turnings once, continuous fermentation
15～20d obtains first fermentation product.
(3) by the liquid humid acid fertilizer azospirillum of slant preservation, Candida utilis, streptomyces microflavus and fixed nitrogen class spore
Bacillus is inoculated into respectively LB fluid mediums, 30 DEG C of shaking table cultures 48h.By the bacterium solution in mass ratio 1～2: 1～2 of above-mentioned 4 kinds of bacterium
: 1～2: 1 uniform mixing, obtain plant growth-promoting composite bacteria agent capable.With first fermentation product as raw material, according to the ratio of 2～5% (w/w)
Example addition plant growth-promoting composite bacteria agent capable, 10～15d of standing for fermentation (period turning is once), you can plant growth-promoting type biology is obtained to be had
Machine fertilizer product.
The technique that bio-organic fertilizer product is prepared in embodiment 5～10 is as shown in table 4 with the index similarities and differences.
Comparative example 2
Organic fertilizer product is prepared using chicken manure and rice husk fermentation：
According to the mixed material for preparing chicken manure and rice husk with the identical mode of embodiment 5～8, instrumentality material C/N 27,
Moisture 60%, without composite fermentation microbial inoculum natural fermentation.Ferment the 4th day, heap temperature rises to 63 DEG C, start turning one
It is secondary, afterwards per 3d turnings once, first fermentation product is obtained after continuous fermentation 19d.First fermentation product is continued into composting, equally
Not being inoculated with plant growth-promoting composite bacteria agent capable carries out ferment in second time.Ferment in second time continue 15d, in 7d turning once, fermenting twice
Complete cycle is 34d.After fermentation ends, according to《Biological organic fertilizer》The property of (NY/T 798-2004) standard detection composting production
Matter, and the seed germination index of tunning is determined, the results are shown in Table 4.
The embodiment 5～8 of table 4 prepares the biological organic fertilizer scheme similarities and differences with comparative example 2
According to embodiment 5～8 and the result of comparative example 2, two sections in the present invention are adopted for raw material using chicken manure and rice husk
The formula bio-organic fertilizer product that step by step prepared by fermentation technology is met in NY/T 798-2004 about the requirement of standard.By not
The composite fermentation microbial inoculum and plant growth-promoting composite bacteria agent capable for adding different ratio and quantity in the present invention with fermentation stage is dramatically increased
Living bacteria count in bio-organic fertilizer product.The testing result of GI is shown, is prepared using the present invention in embodiment 5～8
Bio-organic fertilizer product is higher compared with the degree of becoming thoroughly decomposed of comparative example 2, good to plant growth-promoting effect.
Growth-promoting effect of the biological organic fertilizer to plant is further illustrated below based on embodiment 5 and comparative example 2.
Biological organic fertilizer prepared by embodiment 5 and comparative example 2 is applied in wheat paddock according to the amount of every mu of 200kg, is replaced
30% chemical fertilizer applied amount of generation.Survey result points out that the biological organic fertilizer in applying embodiment 5 is compared with the fertilizer for applying comparative example 2
Oryza sativa L. is set to increase income 16%.
Embodiment 9～12
Biological organic fertilizer is prepared with cattle manure, mushroom residue and functional microorganism microbial inoculum mixed fermentation：
(1) biological organic fertilizer is prepared as fermenting raw materials with cattle manure and mushroom residue, fermentation materials gross mass is 500kg, according to
, than dispensing, both are shown in Table 5 at material propertiess for cattle manure, the weight in wet base of mushroom residue 2: 1.Mixed material C/N ratios are 30, and moisture is
63.9%.
The cattle manure of table 5 and the material propertiess of mushroom residue
| Project | Cattle manure | Mushroom residue |
| Total organic carbon (gkg-1) | 325.4 | 385.1 |
| Total nitrogen (gkg-1) | 1.45 | 0.98 |
| C/N | 22.4 | 39.3 |
| Moisture (%) | 75.8 | 41.5 |
(2) by the Methylotrophic bacillus cereuss of slant preservation, Te Jila bacillus cereuss, Geobacillus stearothermophilus and
Yellow thermophilic anaerobic bacillus cereuss Yunnan subspecies respectively take 2 rings and are inoculated in LB culture fluid, respectively at 30 DEG C, 45 DEG C, 55 DEG C and 60
24h is activated at DEG C.Afterwards culture fluid is seeded to into amplification culture in new LB culture medium by 10% inoculum concentration.Fermentation ends
Afterwards, turf absorption microbial inoculum is added with 2: 1 mass ratio.By Methylotrophic bacillus cereuss for obtaining, Te Jila bacillus cereuss, thermophilic
Hot fat ground bacillus and 4 kinds of microbial inoculums of yellow thermophilic anaerobic bacillus cereuss Yunnan subspecies are mixed by 0.5～1: 1～2: 0.5～1: 1
Composite fermentation microbial inoculum is made in conjunction.
Inoculum concentration composite fermentation microbial inoculum according to 0.5～2% (w/w) adds in the compost material got ready to step (1), mixes
Be mixed it is even after start aerobic fermentation.Heap temperature more than 60 DEG C after turning once, afterwards per 2～3d turnings once, continuous fermentation
15～20d obtains first fermentation product.
(3) by the liquid humid acid fertilizer azospirillum of slant preservation, Candida utilis, streptomyces microflavus and fixed nitrogen class spore
Bacillus is inoculated into respectively LB fluid mediums, 30 DEG C of shaking table cultures 48h.By the bacterium solution in mass ratio 1～2: 1～2 of above-mentioned 4 kinds of bacterium
: 1～2: 1 uniform mixing, obtain plant growth-promoting composite bacteria agent capable.With first fermentation product as raw material, according to the ratio of 2～5% (w/w)
Example addition plant growth-promoting composite bacteria agent capable, 10～15d of standing for fermentation (period turning is once), you can plant growth-promoting type biology is obtained to be had
Machine fertilizer product.
The technique that bio-organic fertilizer product is prepared in embodiment 9～12 is as shown in table 6 with the index similarities and differences.
Comparative example 3
Organic fertilizer product is prepared using cattle manure and mushroom residue fermentation：
According to the mixed material for preparing cattle manure and mushroom residue with the identical mode of embodiment 9～12, sending out without compound
Natural fermentation under the conditions of yeast-like fungi agent.Ferment the 3rd day, heap temperature rises to 65 DEG C, start turning once, afterwards per 3d turnings one
It is secondary, first fermentation product is obtained after continuous fermentation 15d.First fermentation product is continued into composting, plant growth-promoting is not equally inoculated with and is answered
Closing microbial inoculum carries out ferment in second time.Ferment in second time continues 14d, and in 7d, once, fermenting twice complete cycle is 29d for turning.Send out
After ferment terminates, according to《Biological organic fertilizer》The property of (NY/T 798-2004) standard detection composting production, and determine tunning
Seed germination index, the results are shown in Table 6.
The embodiment 9～12 of table 6 prepares the biological organic fertilizer scheme similarities and differences with comparative example 3
It is that raw material is sent out step by step using the two-part in the present invention using cattle manure and mushroom residue according to the result of embodiment 5～8
Bio-organic fertilizer product prepared by ferment technique is met in NY/T 798-2004 about the requirement of standard.By in different fermentations rank
The composite fermentation microbial inoculum and plant growth-promoting composite bacteria agent capable of different ratio and quantity significantly increases biology during Duan Tianjia is of the invention has
Living bacteria count in machine fertilizer product.The testing result of GI shows, using the biological organic of present invention preparation in embodiment 9～12
Fertilizer product is higher compared with the degree of becoming thoroughly decomposed of comparative example 3, good to plant growth-promoting effect.
Growth-promoting effect of the biological organic fertilizer to plant is further illustrated below based on embodiment 9 and comparative example 3.
Biological organic fertilizer prepared by embodiment 9 and comparative example 3 is applied in rice terrace according to the amount of every mu of 300kg, is replaced
50% chemical fertilizer applied amount of generation.Survey result points out that the biological organic fertilizer in applying embodiment 9 is compared with the fertilizer for applying comparative example 3
Oryza sativa L. is set to increase income 32%.
Claims (2)
1. a kind of plant growth-promoting type biological organic fertilizer, by animal dung, adjuvant the composite fermentation microbial inoculum of 0.5～2% (w/w) is inoculated with
Inoculate after ferment 2～5% (w/w) plant growth-promoting microbial inoculum fermentation make, the adjuvant be straw, rice husk, mushroom residue, Testa Tritici,
At least one in carbamide, it is characterised in that：The composite fermentation microbial inoculum is by Methylotrophic bacillus cereuss (Bacillus
Methylotrophicus KACC 13105), Te Jila bacillus cereuss (Bacillus tequilensis ATCC BAA-
819), Geobacillus stearothermophilus (Gebacillus stearothermophilus ATCC 12980) and yellow is thermophilic detests
Oxygen bacillus cereuss Yunnan subspecies (Anoxybacillus flavithermus ssp.yunnanesis CCTCC AB2010187)
Mix according to mass ratio 0.5～1: 1～2: 0.5～1: 1；The plant growth-promoting microbial inoculum is by liquid humid acid fertilizer azospirillum
(Azospirillum humicireducens CCTCC AB 2012021), Candida utilis (Candida utilis
CCTCC M 2010090), streptomyces microflavus (Streptomyces microflavus CGMCC 4.891) and fixed nitrogen class spore
The ratio mixing of bacillus (Paenibacillus azotofixans ATCC 35681) in mass ratio 1～2: 1～2: 1～2: 1
Form.
2. a kind of preparation method of plant growth-promoting type biological organic fertilizer, it is characterised in that：Comprise the following steps that：
(1) prepared by fermentation material：Mixed with least one in straw, rice husk, mushroom residue, Testa Tritici, the carbamide after animal dung and crushing
Close, adjust moisture and C/N respectively to 55%～70% and 25～35, fermentation material is obtained；
(2) high-temperature aerobic fermentation：The composite fermentation microbial inoculum of 0.5～2% (w/w) is inoculated with step (1) fermentation materials, is mixed and is mixed
Even laggard aerobe fermentation of acting charitably；After heap temperature is more than 60 DEG C turning once, afterwards per 2～3d turnings once, continuous fermentation 15
～20d obtains first fermentation product；
The composite fermentation microbial inoculum is by Methylotrophic bacillus cereuss (Bacillus methylotrophicus KACC
13105), Te Jila bacillus cereuss (Bacillus tequilensis ATCC BAA-819), Geobacillus stearothermophilus
(Gebacillus stearothermophilus ATCC 12980) and yellow thermophilic anaerobic bacillus cereuss Yunnan subspecies
(Anoxybacillus flavithermus ssp.yunnanesis CCTCC AB2010187) is according to mass ratio 0.5～1: 1
Mix at～2: 0.5～1: 1；
(3) prepared by plant growth-promoting microbial inoculum：By the liquid humid acid fertilizer azospirillum (Azospirillum being preserved on inclined-plane
Humicireducens CCTCC AB 2012021), Candida utilis (Candida utilis CCTCC M
2010090), streptomyces microflavus (Streptomyces microflavus CGMCC 4.891) and fixed nitrogen series bacillus
(Paenibacillus azotofixans ATCC 35681) is inoculated into respectively LB fluid mediums, under conditions of 28～32 DEG C
24～48h of shaking table culture；By the ratio mix homogeneously of the bacterium solution in mass ratio 1～2: 1～2: 1～2: 1 of above-mentioned 4 kinds of bacterium, must plant
Thing growth-promoting composite bacteria agent capable；
The LB culture medium prescriptions are：Tryptone 10g/L, yeast extract 5g/L, NaCl 10g/L；
(4) become thoroughly decomposed after fertilizer：First fermentation product with step (2) preparation adds as raw material according to the ratio of 2～5% (w/w)
Plus plant growth-promoting composite bacteria agent capable prepared by step (3), 10～15d of standing for fermentation (period turning is once), you can plant is obtained and promotees
Raw type bio-organic fertilizer product.
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| CN106916756A (en) * | 2017-04-10 | 2017-07-04 | 北京绿安创华环保科技股份有限公司 | Fungal bacterial strain, microbial bacterial agent, stalk soil-repairing agent and its application |
| CN107118045A (en) * | 2017-07-11 | 2017-09-01 | 烟台三合生物科技有限公司 | A kind of technology for the complex micro organism fungicide that apple cultivation is used |
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Address after: 044000 Yuncheng Industrial Park, Shanxi Province, Saline Lake Road, the intersection of the 7 Applicant after: Shanxi Ningbang Biotechnology Co., Ltd. Address before: 044000 Yuncheng Industrial Park, Shanxi Province, Saline Lake Road, the intersection of the 7 Applicant before: Shanxi Ningbang biological fertilizer Co. Ltd. |
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Application publication date: 20170510 |