CN106916756B - Fungus strain, microbial agent, straw soil remediation agent and application thereof - Google Patents
Fungus strain, microbial agent, straw soil remediation agent and application thereof Download PDFInfo
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/885—Trichoderma
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F7/00—Fertilisers from waste water, sewage sludge, sea slime, ooze or similar masses
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F9/00—Fertilisers from household or town refuse
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
Abstract
The invention relates to the technical field of microorganism application, in particular to a fungus strain, a microbial agent, a straw soil remediation agent, a preparation method and application thereof. The strain is trichoderma asperellum WL, is preserved in the common microorganism center of China Committee for culture Collection of microorganisms, and has the preservation number as follows: CGMCC NO. 13766; the preservation time is as follows: year 2017, 03 and 23. The strain has the function of enriching heavy metal substances, has a cellulose degradation function, and can be well used for straw degradation and soil remediation. The microbial agent containing the strain is more convenient and effective in straw degradation and subsequent soil application. The straw soil restoration agent is prepared by fermenting the microbial inoculum and the straws, not only overcomes the difficulty of the existing rural straw digestion, but also changes waste into valuable, and the rural waste straws become the soil restoration agent which can restore saline-alkali soil and remove heavy metals and petrochemical pollution sources polluting the soil without any side effect or secondary pollution danger.
Description
Technical Field
The invention relates to the technical field of microorganism application, in particular to a fungus strain, a microbial agent, a straw soil remediation agent, a preparation method and application thereof.
Background
Soil remediation is more difficult than with atmospheric and water pollution. The members of the agricultural product producing area soil heavy metal pollution prevention expert group, China agricultural university resource and Sudepure professor of environmental institute indicate that not only is the soil pollution serious, but also the heavy metal in the soil can not be degraded unlike some organic pollutants, the difficulty of separating from the soil is very large, and the repair is very difficult. The soil heavy metal pollutants are mainly mercury, lead, copper, zinc, chromium, nickel, tin, metal arsenic and the like. At present, the soil treatment technology has a plurality of advantages and disadvantages, and the treatment approaches are mostly physical, chemical and biological methods which can be found from various domestic and foreign literature reports on the treatment of heavy metal pollution. The physical method has good effect, is not limited by soil conditions, but needs a large amount of manpower, material resources and financial resources, has large investment and slow effect taking, and can also damage the soil structure and soil microorganisms; the chemical methods have quick response, but the methods usually have high investment, need complex equipment conditions, disturb soil layer structures, easily bring secondary pollution and the like, and have no possibility of large-area pollution, so the methods cannot fundamentally solve the problems. With the increasing attention of people on environmental protection, a new way for treating heavy metal contaminated soil under the condition of not destroying the physical and chemical properties of the soil is urgently needed to be found, so that the bioremediation is the preferred method.
Straw treatment is the headmost problem in rural areas at present, and burning straws after harvesting seasons every year pollutes the environment, seriously pollutes air and increases PM2.5, and flight can not take off and land in serious cases. Based on this, the government encourages farmers to return straws to the field all the time, and the straw returning is a traditional agricultural measure, and has important significance for improving the organic matter content of soil, maintaining the soil fertility and realizing the sustainable development of agriculture. In recent years, with the development of agricultural mechanization and microbial technology (microbial preparation), the direct returning of straws to fields generally gains attention, and is widely popularized and applied nationwide. The waste phenomenon of straw burning is corrected to a certain extent, the damage to the atmospheric environment and the ecological environment is reduced, the soil fertility is improved, and the organic matter content of the soil is effectively improved.
After the polluted soil is repaired, the polluted soil can not be used for crop growth due to the loss of organic matters in the soil, and a large amount of organic matters can be generated after the straws are degraded by microorganisms, so that the polluted soil is an effective source of soil fertilizers. Therefore, if a technology for effectively combining soil remediation and straw digestion can be provided, the effect of achieving twice the result with half the effort can be undoubtedly achieved.
In view of the above, the present invention is particularly proposed.
Disclosure of Invention
The first purpose of the invention is to provide a fungus strain, which has the function of complexing and enriching heavy metal substances, has a cellulose degradation function, and can be well used for straw degradation and soil remediation.
The second purpose of the invention is to provide a microbial agent containing the strain, so that the strain is more convenient and effective in straw degradation and subsequent soil application.
The third purpose of the invention is to provide a straw soil restoration agent, which not only overcomes the difficulty of the existing rural straw absorption, but also changes waste into valuable, and the rural waste straw becomes a soil restoration agent, which can restore saline-alkali soil, remove heavy metals and petrochemical pollution sources polluting the soil, and has no side effect or secondary pollution.
In order to achieve the above purpose of the present invention, the following technical solutions are adopted:
a fungus strain is Trichoderma asperellum WL and is preserved in the China general microbiological culture Collection center, and the preservation number is as follows: CGMCC NO. 13766; the preservation time is as follows: year 2017, 03 and 23.
The Latin strain is named as Trichoderma asperellum, the Chinese is named as Trichoderma asperellum, the strain is named as WL, and the Latin strain is obtained by screening from heavy metal polluted areas. Through identification, the fungus belongs to fungi of Deuteromycota, Trichosporon and Trichoderma.
The biological characteristics of the strain are as follows: culturing the bacterial colonies on a PDA (personal digital assistant) flat plate at 27-29 ℃, expanding the bacterial colonies to 10cm in 3-4 days, wherein the bacterial colonies are faint yellow and sparse in the initial stage, hyphae grow stolonically on the surface, a dark green spore production area is formed, and the back surface is white; conidiophores are spherical, nearly spherical, single conidiophores are nearly colorless, and the conidiophores are light yellow green when gathered and have smooth walls. The morphological characteristics conform to the characteristics of trichoderma asperellum.
The microbial agent containing the trichoderma asperellum WL or the spores thereof also belongs to the protection scope of the application. In the practical application process, considering the reason that the microorganism bacterium may need to be transported, the microorganism bacterium needs to be expanded and cultured to form a microorganism bacterium agent so as to expand the application range of the microorganism bacterium.
The microbial agent is preferably a liquid agent, and the culture solution in the liquid agent may preferably be a liquid medium, or further added with nutrients such as glucose, thiamine, biotin, nicotinic acid, and the like.
Preferably, the microbial inoculum contains 1 × 10/ml10～12The Trichoderma asperellum WL spores of (1).
Preferably, the microbial agent further comprises 1X 1010～12The viable spores of Geobacillus stearothermophilus.
Geobacillus stearothermophilus is purchased from ThermoFisher under the trade name Culti-LoopsTM Geobacillus stearothermophilus
12980TM, cat # R4601219. The strain can be well matched with trichoderma asperellum WL for fermentation, and the fermentation efficiency is improved.
Preferably, the microbial agent is prepared by mixing the Trichoderma asperellum WL and the Geobacillus stearothermophilus live spores in a ratio of (5-10): 0-10.
The mixing ratio is counted by the number of live spores or live bacteria.
Preferably, the microbial agent further comprises one or more of lactobacillus plantarum, trichoderma reesei, lactobacillus delbrueckii, methanothermophilus, thermoanaerobacter metallophilus, thermoactinomyces albus, bacillus subtilis, thermophilic sporotrichum, bacillus licheniformis, bacillus stearothermophilus, bacillus cereus and bacillus citrate.
A novel method for digesting straws uses the microbial agent to digest straws.
A straw soil remediation agent is prepared by fermenting the microbial agent and a fermentation mixture;
the fermentation mixture comprises straw, sludge and domestic organic garbage.
The sludge can provide a necessary culture mechanism for the growth of microorganisms, has the function of keeping moisture, and the organic garbage can provide rich carbon sources and nutrient substances for the fermentation of the microorganisms, so that the microorganisms in the microbial inoculum provided by the application can rapidly grow to a logarithmic phase, and the fermentation time is shortened.
Preferably, the straw soil remediation agent is a crushed particle with the particle size of 1-5 mm.
Preferably, the straw soil remediation agent comprises the following components in percentage by mass (5-8): (1-5): (1-5);
preferably, the water content of the fermentation mixture is 40% to 60%.
The domestic organic garbage refers to the waste containing organic components in the domestic garbage, and comprises pericarp, vegetable peel and leaf, leftover, wood, waste paper, vegetable peel and the like.
The straw soil remediation agent is applied to in-situ remediation of saline-alkali soil.
The straw soil remediation agent is applied to soil heavy metal remediation.
Compared with the prior art, the invention has the beneficial effects that:
(1) changing waste into valuable. Solves the difficult problem of straw treatment and changes garbage into resources. Simultaneously, the urban organic garbage can be utilized during fermentation; has the advantages of production and application: low investment, low energy consumption and wide sources.
(2) Solves the problems of heavy metal pollution of land and saline-alkali land, and restores the soil into good soil. The straw soil restoration agent contains a large amount of beneficial bacteria and various elements required by plant growth, adsorbs biological organic inorganic fertilizer of soil beneficial bacteria such as high-efficiency azotobacter, phosphorus-dissolving bacteria, potassium-dissolving bacteria, antagonistic bacteria and the like, has a better promotion effect on the growth of crops than the prior compost, is beneficial to improving the quality of agricultural products and improving the economic benefit.
(3) The straw soil remediation agent disclosed by the invention is cheap in raw materials, simple in process, suitable for large-scale industrial application and strong in practicability.
The Trichoderma asperellum provided by the application has a strain name of WL, is preserved in the China general microbiological culture Collection center of the culture Collection of microorganisms with the preservation addresses of: western road No.1, north west city of township, beijing, institute of microbiology, china academy of sciences; the preservation time is as follows: 23.03.2017, and the preservation number is CGMCC NO. 13766. The strains were detected as viable by the depository at 23.03.2017.
Detailed Description
Embodiments of the present invention will be described in detail below with reference to examples, but it will be understood by those skilled in the art that the following examples are only illustrative of the present invention and should not be construed as limiting the scope of the present invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by manufacturers, and are all conventional products available on the market.
Example 1
The embodiment provides a microbial agent containing trichoderma asperellum spore powder and a preparation method thereof, wherein the method comprises the following steps:
firstly, inoculating the preserved strain on a PDA culture medium plate, performing activation culture at 28 ℃ until spore is produced, and sieving the obtained spore powder (200 meshes) to obtain the trichoderma asperellum spore powder;
preparing spore powder into powder with concentration of 1 × 1011And (4) suspending each spore/mL to obtain a microbial liquid inoculum.
The PDA culture medium comprises the following components in part by weight: weighing 200g of potato, cleaning, peeling, cutting into small pieces, adding 1000ml of water, boiling for half an hour, filtering with gauze, adding 10-20 g of glucose, fully dissolving, fixing the volume to 1000ml, and sterilizing at 121 ℃ for 30min.
Example 2
The embodiment provides a microbial agent containing trichoderma asperellum spore powder and a preparation method thereof, wherein the method comprises the following steps:
1) firstly, inoculating the preserved strain on a PDA culture medium plate, performing activation culture at 28 ℃ until spore is produced, and sieving the obtained spore powder (200 meshes) to obtain the trichoderma asperellum spore powder;
preparing spore powder into powder with concentration of 1 × 1011And (4) suspending each spore/mL to obtain a first microbial liquid inoculum.
The PDA culture medium comprises the following components in part by weight: weighing 200g of potato, cleaning, peeling, cutting into small pieces, adding 1000ml of water, boiling for half an hour, filtering with gauze, adding 10-20 g of glucose, fully dissolving, fixing the volume to 1000ml, and sterilizing at 121 ℃ for 30min.
2) Culturing and counting Geobacillus stearothermophilus according to the description convention, and suspending with a solvent to obtain a second microbial liquid agent;
the solvent formulas of the first microbial liquid inoculant and the second microbial liquid inoculant are as follows: 8 to 20 percent of emulsifier Geronol VO/01, 0.5 to 5 percent of sodium carboxymethylcellulose, 10 to 15 percent of calcium lignosulfonate, 0.1 to 3 percent of butyl hydroxy anisole, 3 to 10 percent of organic bentonite, 0.1 to 0.6 percent of dextrin, 0.1 to 2 percent of preservative and 24.4 to 77.7 percent of vegetable oil.
3) Mixing the first microbial liquid inoculum and the second microbial liquid inoculum, and measuring that the content of trichoderma asperellum spores in the obtained liquid inoculum is 1 multiplied by 1011One per ml. The mixing ratio of the trichoderma asperellum spores to the geobacillus stearothermophilus live spores is 8: 5.
Example 3
In this example, the preparation procedure was the same as that of example 2, except that in this example, in addition to Geobacillus stearothermophilus, Lactobacillus plantarum, Lactobacillus delbrueckii, Micrococcus methanothermophilus, Thermoactinomyces albus and Citrobacter were added, and a composite bacterial solution comprising the above-mentioned bacterial strains in an equal ratio of bacterial solution volume ratio was added, and the bacterial content was 1-9X 1010One per ml.
Example 4
The embodiment provides a solid microbial inoculum and a preparation method thereof, and the preparation method specifically comprises the following steps:
the liquid microbial inoculum prepared in the embodiment 3 is added into talcum powder according to the proportion that 20g of talcum powder (used as a solid carrier) is added into 1 kg of liquid microbial inoculum, and the mixture is fully stirred, uniformly mixed, filtered, dried and crushed to obtain the solid microbial inoculum.
Example 5
In this example, the preparation was carried out in the same manner as in example 4, except that in this example, the solid carrier used was a mixture of powdered milk, talc, fine sand and clay in equal proportions.
Example 6
The embodiment provides a preparation method of a straw soil remediation agent, which comprises the following steps:
the straw and branch tree leaf soil remediation agent is prepared by performing two-step microbial fermentation on straw, sludge and domestic organic garbage, and the method is simple and low in cost.
1) And raw material pretreatment:
a. removing impurities such as plastics, metals, bones and the like in the fermentation raw materials;
b. the method comprises the following steps of (1) putting straws into a crusher, and crushing to obtain a raw material with the particle size of 1-5 cm;
c. mixing the crushed straws with other fermentation raw materials, wherein the mixing mass ratio of the straws to the sludge to the domestic organic garbage is 6: 3: 3. and (3) putting the mixed materials into a stirrer, adding water to adjust the water content, and stirring to ensure that the water content of the fermented materials is between 40 and 60 percent.
2) And adding the fermented material obtained in the previous step into a fermentation tank, adding anaerobic rumen microbial bacteria and ciliates, and uniformly stirring for pre-fermentation. And (3) keeping the temperature of 25-35 ℃ for natural fermentation for 2-3 days, basically decomposing the fermentation body after rapid fermentation, and decomposing the wood fiber. Raising the temperature in the tank to 70-80 ℃ and keeping the temperature for 2-3 days to kill grass seeds and harmful bacteria; when the temperature in the tank is reduced to 30-35 ℃, adding 5-10% of yeast and 5-12% of silicate bacteria, uniformly stirring, controlling the temperature to be 30-35 ℃, and continuing to ferment for 3-4 days; filtering with a filter press; the obtained filtrate can be used as fermentation flora which can be reused. The filter cake can be used as a soil base material to be dried and added with strains for fermentation in the subsequent process.
3) And adding the microbial inoculum prepared in the embodiment 2 to ferment the fermentation product obtained at the upper part for 2-3 days again, and uniformly mixing the microbial inoculum and the fermentation product obtained in the previous step according to the weight ratio of 1: 1000. Meanwhile, the temperature is raised to 55-85 ℃ for a short time, and the temperature is kept for 48 hours to kill the germ components again.
4) And dehydrating and airing the product obtained after fermentation to enable the water content to be lower than 15%, crushing the crude product to the particle size of 1-2 mm, and packaging to prepare the straw soil remediation agent.
Experimental example 1
The straw soil remediation agent prepared in the embodiment 6 of the invention is used for soil improvement test.
The straw soil remediation agent provided by the invention is used for carrying out improvement tests on sludge piles of a breeding farm polluted plot A area and a sewage treatment plant in Beijing City in the great Xingdistrict of Beijing City. The specific operation is as follows: 2 mu of wasteland is selected from two areas respectively to be used as a test field and the heavy metal content of the soil is detected. The product is applied in 10 months in 2014, ploughing and flood irrigation are carried out later, seeding is carried out in 5 days in 6 months, and the crop is eggplant. And (4) taking a soil sample two days before sowing, carrying out nutrient detection again, and measuring the rate of emergence two weeks after sowing. The soil conditioner provided in the examples 2 to 4 is applied to the test sample plots 1 to 3, and the application method is one-time broadcasting, the conditioner is uniformly broadcasted on the surface layer of the soil and ploughs the soil by 0cm to 20cm, so that the soil remediation agent and the soil are uniformly mixed, and the dosage of the soil remediation agent per mu is 300 kilograms.
According to statistics, the eggplant emergence rate of the A-area eggplant of the big-xing area farm in Beijing is 92%, and the eggplant emergence rate of the sludge heap of the sewage treatment plant in Beijing is 93%. The yield of the eggplants can also reach 80 to 90 percent of the yield of normal land.
And if the crops are normally planted, the soil remediation agent can be used as a fertilizer because the soil remediation agent is obtained by fermenting straws. The straw soil remediation agent prepared in the example 6 is directly applied, the yield of the corn can be remarkably increased, the yield per unit is the highest when the straw soil remediation agent is applied to the corn base fertilizer, the yield per mu reaches 559.04 kg/mu, and the yield is increased by 21.23% compared with the yield of 461.13 kg/mu obtained by the traditional cultivation method applying nitrogen, phosphorus and potassium fertilizers. From the point of particle fullness, the thousand-grain weight reaches 434.62g, which is 14.59% higher than 379.26g of the traditional cultivation method applying nitrogen, phosphorus and potassium fertilizers. The difference is not significant from the plant height.
While particular embodiments of the present invention have been illustrated and described, it would be obvious that various other changes and modifications can be made without departing from the spirit and scope of the invention. It is therefore intended to cover in the appended claims all such changes and modifications that are within the scope of this invention.
Claims (9)
1. A microbial inoculum, characterized in that the inoculum contains 1 x 10 per ml10～12The Trichoderma asperellum WL spore of (1X 10) is also contained in each milliliter of the microbial agent10～12The viable spores of Geobacillus stearothermophilus;
the Trichoderma asperellum (Trichoderma asperellum) WL is preserved in the common microorganism center of China Committee for culture Collection of microorganisms, and the preservation number is as follows: CGMCC NO. 13766; the preservation time is as follows: 23/03/2017;
the mixing ratio of the trichoderma asperellum WL to the geobacillus stearothermophilus live spores is (5-10): 0-10.
2. The microbial agent according to claim 1, further comprising one or more of Lactobacillus plantarum, Trichoderma reesei, Lactobacillus delbrueckii, Micrococcus methanothermophilus, Thermoanaerobacter metallophilus, Thermoactinomyces albus, Bacillus subtilis, Thermomyces thermophilus, Bacillus licheniformis, Bacillus cereus, and Bacillus citrate.
3. A new method for the consumption of straw, characterized in that the microbial inoculant according to claim 1 or 2 is used to consume straw.
4. A straw soil remediation agent, wherein the straw soil remediation agent is prepared by fermenting the microbial agent of claim 1 or 2 with a fermentation mixture;
the fermentation mixture comprises straw, sludge and domestic organic garbage.
5. The straw soil remediation agent of claim 4, wherein the straw is ground particles with a particle size of 1-5 mm.
6. The straw soil remediation agent of claim 4, wherein the mass ratio of the straw, the sludge and the domestic organic garbage is (5-8): (1-5): (1-5).
7. The straw soil remediation agent of claim 4, wherein the fermentation mixture has a moisture content of 40% to 60%.
8. The use of the straw soil remediation agent of any one of claims 4 to 6 in the in situ remediation of saline and alkaline soil.
9. The use of the straw soil remediation agent of any one of claims 4 to 6 in soil heavy metal remediation.
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| CN108277166B (en) * | 2018-03-27 | 2021-11-30 | 山东农业大学 | Trichoderma asperellum and application thereof in lead-contaminated soil remediation |
| CN108949587B (en) * | 2018-08-14 | 2022-01-14 | 北京绿安创华环保科技有限公司 | Microbial agent, organic garbage sandy soil remediation agent, preparation method and application thereof |
| CN109160861A (en) * | 2018-10-31 | 2019-01-08 | 周口师范学院 | A kind of simple trichoderma-straw bacterial manure preparation method |
| CN115011353A (en) * | 2022-06-28 | 2022-09-06 | 黑龙江八一农垦大学 | Application of trichoderma asperellum in repairing saline-alkali farmland soil and/or promoting plant growth |
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