CN102391876A - Composite biological soil modifier and application thereof - Google Patents
Composite biological soil modifier and application thereof Download PDFInfo
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- CN102391876A CN102391876A CN2011102923421A CN201110292342A CN102391876A CN 102391876 A CN102391876 A CN 102391876A CN 2011102923421 A CN2011102923421 A CN 2011102923421A CN 201110292342 A CN201110292342 A CN 201110292342A CN 102391876 A CN102391876 A CN 102391876A
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- soil
- compound biological
- bacillus subtilis
- fermentation
- trichoderma pseudokiningii
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Abstract
The invention discloses an unpolluted, green and high-efficiency composite biological soil modifier which comprises the following components: trichoderma pseudokoningii fermentation liquor, bacillus subtilis fermentation liquor, water-soluble chitin, potassium humate and decomposed organic matter. The trichoderma pseudokoningii and the bacillus subtilis are high-efficiency bacterial strains which are obtained through screening and mutating, the trichoderma pseudokoningii fermentation liquor and the bacillus subtilis fermentation liquor are obtained by a liquor submerged fermentation technology, and the unpolluted, green and high-efficiency composite biological soil modifier can be prepared in a combination way. The soil modifier has the following functions that the physicochemical property of the soil can be improved, the hardening of the soil can be broken up, the volume weight of the soil can be reduced, the air permeability of the soil can be improved, the microbiological activity of the soil can be promoted, the growth of the plant root system can be promoted, the disease resistance of the crop can be enhanced, the yield of the crop can be improved, the quality of the crop can be improved, the original ecology of the crop can be recovered and the like, and the soil modifier is the novel soil modifier which is high-efficiency, environment-friendly and convenient to use.
Description
Technical field
The present invention is a kind of compound soil modifier; Specifically; Be the center and add potassium fulvate and decomposed organic matter with active composite bacteria, both can improve soil physico-chemical property, provide crop growth necessary nutritive substance; Can suppress Plant diseases again, be a kind of soil improvement agent of green high-efficient.
Background technology
Soil is the important carrier of plant growth environment, and it provides photosynthetic raw material and attaching substratum to plant, and soil quality and crop yield are closely bound up.Soil quality is meant naturally or in the ecosystem boundary of labor management, soil has plant-animal production persistence, keeps and improve the ability of water, makings amount and human health and life.The basis of soil quality is a soil fertility, and soil fertility is that soil is the ability that plant-growth provided and coordinated nutritional condition and envrionment conditions, is the general performance of the various essential propertys of soil.The measure of increasing soil fertility comprises: applying organic manure material, green manuring and rational use of chemical fertilizer, these measures not only help the high yield of this season crop, and help the recovery and the raising of soil fertility.Current; Because the Soil degradation problem that various irrational mankind's activities (depending on unduly and irrational use chemical fertilizer, excessively cultivation, single cropping system etc.) and natural cause cause serious threat causes Soil degradation to the sustainable development of agricultural.Soil degradation can be divided into three types of physical degradation, chemical degradation and biodeteriorations, shows as mainly that the soil erosion, the soil salinization and compacting are hardened, soil acidification, the soil organism run off, heavy metal pollution of soil poisons etc.Current, the main forms of China's Soil degradation is the soil erosion, soil compaction.According to statistics, China's soil erosion area in 1996 reaches 1,830,000 surprising km
2, account for 19.00% of area, wherein southern red yellow earth area soil erosion area is 6.15 * 10
5Km
2, account for 25.00% of soil, this area total area.The Southern Red Soil district has 2 * 10 at present approximately
5Km
2Soil serious acidifying situation appears.The soil erosion, soil compaction have had a strong impact on the development of China's agriculture prodn.
The soil erosion is also referred to as soil erosion; Refer to that topsoil or soil parent material are under effects such as water, wind, gravity; The various forms of phenomenons of degrading, carrying and piling up taking place, cause topsoil loss, soil desertification, soil compaction compacting, is the most important factor that influences Soil degradation.Vegetation deterioration that human activity causes and soil are exposed to be the major reason of the aggravation soil erosion.
Soil compaction is that Soil structure destroys under rainfall or external cause action such as pour water, and gathers the phenomenon of compacting in the dry back.Applying excessive nitrogenous fertilizer in the soil, cause the too much soil organic substance of microbial consumption, influence microorganism active, destroy the soil aggregation body structure, is the major reason of soil compaction.
From 19 end of the centurys so far, in order to solve the Soil degradation problem, scientific worker's door of country variant has been developed multiple soil improvement agent.Can it be divided into natural modifying agent, synthetic modifying agent and biological modification agent according to its starting material source.Natural modifying agent is like natural mineral, inorganic and organic solid castoff, extraction high molecular polymer.Synthetic modifying agent is the high molecular polymer of the 26S Proteasome Structure and Function synthetic of the natural modifying agent of simulation, like SEPIGEL 305, polyvinyl alcohol resin, Z 150PH, urea-formaldehyde resin etc.In production application, natural modifying agent and synthetic modifying agent exist some theoretical and practical problems and negative effects.As: flyash has stronger absorption fixed action to P; Organic waste contains objectionable impuritiess such as various pathogenic bacterias, heavy metal, polychlorobiphenyl; Higher and the intermediate product acrylic amide its degraded of the production cost of SEPIGEL 305 is poisonous or the like.
The biological modification agent of current China of a great variety, mixed and disorderly, but be mostly extensive routine techniques, bacterial classification and the no specific aim of prescription; The effective constituent standard of product is chaotic; Quality product and practical application effect are undesirable, and seriously polluted in the production process, and the large-scale promotion application example is less.The separation screening of high-efficiency strain, purifying cultivate problem, production cost and pollution control and the environmental safety assessment still is the bottleneck of restriction biological modification agent development.
Summary of the invention
To problem such as Soil degradation and existing soil improvement agent effect be not good, the object of the present invention is to provide a kind of pollution-free, efficient, practical compound biological soil improver, the present invention also provides the application method of this soil improvement agent.
The component of compound biological soil improver of the present invention comprises trichoderma pseudokiningii fermented liquid and fermentation of bacillus subtilis liquid.
Compound biological soil improver of the present invention comprises following main ingredient:
The trichoderma pseudokiningii fermented liquid
Fermentation of bacillus subtilis liquid
N,O-CMC
Potassium fulvate
Employed trichoderma pseudokiningii fermented liquid in the compound biological soil improver according to the invention, be by trichoderma pseudokiningii (
Trichoderma pseudokiningii) TRIPI fermentation makes.
The trichoderma pseudokiningii that the present invention selected for use (
Trichoderma pseudokiningii) TRIPI be from stalk, separate, then through the novel strain of mutagenesis, screening, the called after trichoderma pseudokiningii (
Trichoderma pseudokiningii) TRIPI.This bacterial strain carries out preservation at the common micro-organisms center C GMCC of China Committee for Culture Collection of Microorganisms; Preserving number is CGMCC NO.1443; The preservation time is on August 22nd, 2005, and the preservation address is the No. 13, North No.1 Row, Zhongguancun, Haidian District, Beijing City, Institute of Microorganism, Academia Sinica.
The inventor will press the isolated trichoderma pseudokiningii original strain of usual manner from the stalk of experimental plot, handle (1 * 10 through ultraviolet secondary complex mutation
6The bacteria suspension of individual/ml is put in the petridish of diameter 9 cm; Uv lamp preheating 20 min; With petridish as on the magnetic stirring apparatus and make petridish about 10W uv lamp vertical range 25 cm; The irradiation 90S), filter out bacterial strain with higher mycelial growth rate, cellulase activity and bacteriostatic activity, be the trichoderma pseudokiningii that preserving number provided by the present invention is CGMCC NO.1443 (
Trichoderma pseudokiningii) TRIPI.
Trichoderma pseudokiningii of the present invention (
Trichoderma pseudokiningii) cultural method of TRIPI is:
1. with transfering loop with cryopreserved trichoderma pseudokiningii TRIPI bacterial classification inoculation in dull and stereotyped PDA substratum (yam 200g, sucrose 20g, agar 20g, zero(ppm) water 1000mL), transferred for 2 generations; 2. transfering loop picking edge mycelia is transferred in PDB substratum (yam 200g, sucrose 20g, zero(ppm) water 1000mL); 25 ℃～30 ℃ of culture temperature; Optimum temperuture is 28 ℃, 150rpm, and the time is 7～10 days; Fermented liquid 100 μ m sieve filters and obtains spore suspension, and the spore concentration of living is 3-9 * 10
8Individual/ml.
This bacterial strain that filters out was transferred for 10 generations continuously its good character inheritance stability.
Table 1 trichoderma pseudokiningii mutagenesis result
Starting strain and mutant strain shake-flask culture mycelia dry weight and fermented liquid (1000r/min, centrifugal 10min) filter paper enzyme (FPA), endo-type-beta-glucanase (C1 enzyme) after 5 days, circumscribed-type-beta-glucanase (CBH enzyme) is active and potted plant experiment in to the cucumber verticillium wilt pathogen (
Fusarium oxysporum f.sp. cucumerinum), withered germ of water-melon (
Fusarium oxysporum f.sp. niveum) control effect.
Employed fermentation of bacillus subtilis liquid in the compound biological soil improver according to the invention, be by subtilis (
Bacillus subtilis) BACI fermentation makes.
Subtilis among the present invention (
Bacillus subtilis) BACI be from the plant rhizosphere soil, separate, then through the novel strain of mutagenesis, screening, the called after subtilis (
Bacillus subtilis) BACI.This bacterial strain carries out preservation at the common micro-organisms center C GMCC of China Committee for Culture Collection of Microorganisms; Preserving number is CGMCC NO.1442; The preservation time is on August 22nd, 2005, and the preservation address is the No. 13, North No.1 Row, Zhongguancun, Haidian District, Beijing City, Institute of Microorganism, Academia Sinica.
The inventor will press the isolated subtilis original strain of usual manner from the plant rhizosphere soil of experimental plot, through ultraviolet secondary complex mutation (1 * 10
7The bacteria suspension of individual/ml is put in the petridish of diameter 9 cm; Uv lamp preheating 20 min; With petridish as on the magnetic stirring apparatus and make petridish about 20 W uv lamp vertical ranges, 30 cm; Irradiation 150S) filter out have higher growth rate, the bacterial strain of 'beta '-mannase, beta glucan enzyme, proteolytic enzyme and bacteriostatic activity, be the subtilis that preserving number provided by the present invention is CGMCC NO.1442 (
Bacillus subtilis) BACI.
Subtilis among the present invention (
Bacillus subtilis) cultural method of BACI is: adopt the liquid submerged fermentation best cultivation to carry out fermentation culture and obtain bacillus subtilis spore
1. with the subtilis of preservation (
Bacillus subtilis) the BACI bacterial classification, streak inoculation is in beef-protein medium (Carnis Bovis seu Bubali cream 0.3g, peptone 1g, sodium-chlor 0.5g, agar 2g, zero(ppm) water 100ml, pH7.0～7.2) activation 24h; 2. transfering loop picking thalline is inoculated in LB substratum (tryptone 10g, yeast extract 5g, NaCl 10g, zero(ppm) water 1000ml), enlarged culturing 24h; 3. be inoculated in (yam 200g, glucose 20g, zero(ppm) water 1000ml) in the PDB substratum with 1% inoculum size; Fermentation condition is: 37 ℃ of temperature, and pH=7, dissolved oxygen concentration maintains the 20-30% saturation ratio; 220rpm, incubation time are 2～3 days, and effectively cell concentration is 1-3 * 10
9Individual/ml.
This bacterial strain that filters out was transferred for 10 generations continuously its good character inheritance stability.
Table 2 subtilis mutagenesis result
Behind starting strain and the mutant strain shake-flask culture 24h, in fermented liquid (8000r/min, centrifugal 20min) 'beta '-mannase, beta-glucanase, protease activity and the potted plant experiment to the cucumber verticillium wilt pathogen (
Fusarium oxysporum f.sp. cucumerinum), withered germ of water-melon (
Fusarium oxysporum f.sp. niveum) control effect.
Trichoderma pseudokiningii is a kind of bacterial strain that soil improves potentiality that has very much, distributes extensively, and growth potential is vigorous.It is varied that its soil improves mechanism.Trichoderma pseudokiningii has abundant mycelia, can adhere to and hand over around soil particle, improves soil stability and permeability, breaks soil compaction; Trichoderma pseudokiningii also can efficiently express β-1; Highly active specific enzymes such as 4-glucan hydrolase, chitinase, cellulase, proteolytic enzyme system; Outer macromolecule polysaccharide of born of the same parents and gp that secretion is abundant; Can improve soil physico-chemical property, the release of promotion soil nutrient and root system of plant promote plant root growth to the absorption of nutritive ingredient; Produce the active substance of volatility or non-volatile inhibition pathogenic bacteria growth; Suppress the harmful microbe growth like sticking broom toxin, coal Buddhist nun toxin and victory peptide element etc.; β-1; 4-glucan hydrolase and chitinase have the intensive hydrolytic action to the cell walls of plant pathogenic fungi, cause the disintegration of pathogenic agent and suppress the sprouting of pathogenic bacteria spore and gemma.In addition, trichoderma pseudokiningii flexibility is extremely strong, all can grow fast between 4 ℃ ~ 42 ℃ He under the multiple edatope, can capture site, space and nutritional resource fast.
Subtilis, its resistance under the gemma state is extremely strong, can antioxidant, various contrary property environment such as high temperature.Subtilis can produce the abundant physiologically active substance with high vigor and control of plant disease function in process of growth; Comprise proteolytic enzyme, glycase, cellulase, LSD, polygalacturonase, VITAMINs, chitinase, amino acid, somatomedin, multiple compound such as lipopeptide antibiotic, iturin, Bio-surface active element; They have the promotion decomposition of soil organic matter; Improve fertile ability of soil control and soil enzyme activities, improve microbial bacteria group structure in the soil, promote plant root growth; Suppress effects such as harmful fungoid, bacterium, virus and bacterium substance, wherein the surfactivity element has the activity of inhibition to the various plants pathogenic bacteria.Its meta-bolites strong stress resistance can be played effectiveness for a long time, reduces consumption, both can increase soil fertility, and promotes plant strain growth, can prevent disease again, improves crop yield.
Trichoderma pseudokiningii and subtilis all can secrete the active substance that exocellular polysaccharide, organic acid, gp, VITAMINs, specific enzymes system etc. have certain physiological function in process of growth; Because the morphological properties of two kinds of bacterial classifications is different, thereby, soil physico-chemical property improved; Promote the growth of root system of plant; Enhance root system is regulated rhizosphere soil microorganism flora structure many-side to the receptivity of nutritive ingredient, can play complementation and enhancement.And a large amount of trichoderma pseudokiningii mycelia can with firm the adhering to and twine of soil particle, improve soil stability and porosity.
Chitin has " plant vaccine ", and use can induce farm crop self to produce the resistance factor to disease continuously, reduces the disease incidence, significantly reduces the agricultural chemicals usage quantity.It also has extremely strong blade face adhesion property in addition, can urge root strong sprout, promotes fruit maturation.
Potassium fulvate has high capacity amount and physiologically active, and its chelating constant and trace nutrient can make it be plant utilization better, excite plant microcosmic biological activity; Can slow release fertilizer, improve the absorption of plant to nutrition, promote plant germination and growth; Accelerate precipitation decomposes, and strengthens waterlogging-resistant property.
Chitin and potassium fulvate carry out chelating with its various structure form, play mutual promoting action, more help the improvement of Soil structure.
For comprehensive and screening trichoderma pseudokiningii, subtilis, chitin, the special effect of potassium fulvate and mutual synergism and best complex proportioning; The inventor adopts orthonormal design of experiments, and the quality proportioning umber of each component in the said compound biological soil improver is optimized.
It is Japanese sweet treasured that confession studies thing, and seed is produced by Qingdao Japan sweet precious seed production base, and experiment location, field is positioned at Zhaoyuan City, and orthogonal experiment design and experimental result are seen table 3, table 4.
The table 3 orthogonal experiment factor and horizontal L
9(3
4)
Table 4 Orthogonal experiment results L
9(3
4)
| The experiment number | The A factor | The B factor | The C factor | The D factor | The soil weight (g/cm 3） | Muskmelon output (kg/ mu) | Soil activation organic (g/kg) |
| 1 | 1 | 1 | 1 | 1 | 1.55 | 3190 | 5.6 |
| 2 | 1 | 2 | 2 | 2 | 1.58 | 3005 | 6.3 |
| 3 | 2 | 1 | 2 | 3 | 1.51 | 3299 | 6.4 |
| 4 | 2 | 2 | 3 | 1 | 1.49 | 3364 | 7.1 |
| 5 | 2 | 3 | 1 | 2 | 1.50 | 3308 | 6.9 |
| 6 | 3 | 1 | 3 | 2 | 1.48 | 3469 | 7.5 |
| 7 | 3 | 2 | 1 | 3 | 1.46 | 3371 | 8.2 |
| 8 | 1 | 3 | 3 | 3 | 1.50 | 3505 | 7.3 |
| 9 | 3 | 3 | 2 | 1 | 1.43 | 3672 | 8.6 |
| Soil weight K1 | 1.47 | 1.51 | 1.50 | 1.51 | |||
| K2 | 1.54 | 1.52 | 1.50 | 1.49 | |||
| K3 | 1.49 | 1.46 | 1.50 | 1.50 | |||
| Extreme difference R | 0.07 | 0.06 | 0.01 | 0.02 | |||
| Muskmelon output k1 | 3483.00 | 3287.33 | 3370.67 | 3228.00 | |||
| k2 | 3233.33 | 3257.67 | 3343.33 | 3446.00 | |||
| k3 | 3344.67 | 3516.00 | 3347.00 | 3387.00 | |||
| Extreme difference R | 249.67 | 258.33 | 27.33 | 218.00 | |||
| The organic k1 of soil activation | 7.67 | 6.53 | 6.87 | 7.13 | |||
| k2 | 6.40 | 6.73 | 7.10 | 7.30 | |||
| k3 | 7.23 | 8.03 | 7.33 | 6.87 | |||
| Extreme difference R | 1.27 | 1.50 | 0.47 | 0.43 |
Can find out that by the land for growing field crops Orthogonal experiment results four kinds of components all exert an influence to soil property and crop yield.Each factor pair soil weight influence order is successively: A＞B＞D＞C; And to muskmelon yield effect sequence of operation be successively: B＞A＞D＞C; And to soil activation organic content influence order be successively: A＞B＞C＞D.It is thus clear that trichoderma pseudokiningii and subtilis play decisive role to soil property and crop yield in the compound preparation; Trichoderma pseudokiningii content is the most remarkable to the soil weight and the influence of soil activation organic content, and subtilis is then remarkable to the influence of muskmelon output.Can find with the soil weight to be that screening targets is a screening targets with the soil activation organic matter perhaps by the result, be screening targets with muskmelon output still, No. 9 level of factor A
3B
3C
2D
1, be the optimal proportion under this experiment condition.
According to the result of orthogonal test, and take into account the multiple factor in processing and the use simultaneously, the inventor confirms that the parts by weight of each main ingredient are following in the compound biological soil improver according to the invention:
Trichoderma pseudokiningii fermented liquid 38-42 part, fermentation of bacillus subtilis liquid 38-42 part, N,O-CMC 0.5-1.5 part, potassium fulvate 0.5-1.5 part;
Wherein, effective spore concentration>=3 * 10 in the trichoderma pseudokiningii fermented liquid
8Individual/ml; Fermentation of bacillus subtilis liquid gemma number>=1 * 10
9Individual/ml; N,O-CMC: molecular weight M is about (1-5) * 10
5, viscosity 100-200mPa takes off ethyl ester degree DAC>=90%, purity>=90%; The potassium fulvate specification is: fineness＜120 orders, water-soluble substance＞99.7%, water-soluble xanthohumic acid＞50%, potassium oxide 9.5-10%, nitrogen 2.5-3.0%, phosphorus 0.5-0.8%, organic 50-60%.(wherein percentage composition is weight %)
For improving this preparation at the germination rate of using initial stage trichoderma pseudokiningii spore and subtilis, can suitably add decomposed organic matter commonly used, its interpolation weight is 5～20 parts and is advisable.
In above-mentioned weight consumption proportional range, compound biological soil improver of the present invention all can obtain comparatively ideal soil improvement and protection effect.
The preparation method of compound biological soil improver of the present invention is following:
By required parts by weight trichoderma pseudokiningii spore and fermentation of bacillus subtilis liquid are mixed; Then N,O-CMC is evenly added in the bacterium liquid and dissolve; Again potassium fulvate is added in the mixed solution, mix, be processed as stable liquid compound biological soil improver.The chlorogenicacid isoreactivity material that also can add the marine bacteria meta-bolites simultaneously and from halophytes, extract.
Compound biological soil improver consumption of the present invention is: new stub land 500-1500ml/ mu, old stub land 1500-2500ml/ mu.
Concrete application mode is: with liquid soil improvement dilution agent 100-200 doubly, before field planting, evenly spray and turn over behind the soil surface or irritate root.
Compound biological soil improver of the present invention is applicable to the improvement of multiple soil such as rice soil, hydromorphic soil, pedocal, alfisol, saline-alkali soil.
Compound biological soil improver of the present invention receives more and more agricultural science and technology workers' favor with its soil improvement ability efficiently and crop yield ability, and microorganism species is an important function component wherein.Aspects such as soil microorganisms forms at soil, the degraded of the circulation of the biochemistry of the ecosystem, extraneous contamination material and plant stress-resistance have vital role; Can improve the physico-chemical property of soil; The formation of promotion soil aggregate and stable; Mineral nutrient in the activating soil, the microenvironment of effectively improving the soil, the dietetic alimentation ability of enhancement of plant and its disease resistance, resistance etc.，
Compound biological soil improver of the present invention all has tangible production-increasing function to various crop such as muskmelon, wheat, navel oranges.
The present invention is the center component with the novel trichoderma pseudokiningii and the subtilis of separation, mutagenesis, screening, and adds chitin, potassium fulvate and decomposed organic matter, is intended to through improving the physico-chemical property of soil; Break soil compaction and compacting; Promote the decomposition of the soil organism and the release of nutritive ingredient, improve the fertile ability of soil control, regulate soil pH; Regulate the microbial bacteria group structure; Suppress the harmful microbe growth, the enhancement of plant root system is to the receptivity of nutritive substance and promote its growth, reaches to improve the soil and increase production purpose.Two kinds of bacterial strain reproduction speeds using among the present invention are very fast, and it is active to have high exocytosis, utilizes the liquid submerged fermentation technology to realize large-scale production; Can obtain a large amount of thalline and spore fast; Reduced production cost, and product is liquid preparation, easy to use.
Embodiment
One. soil improvement agent liquid preparation amounts of components embodiment of the present invention is following:
FORMULATION EXAMPLE 1
Component and parts by weight:
38 parts of trichoderma pseudokiningii fermented liquids, 42 parts of fermentation of bacillus subtilis liquid, 0.5 part of N,O-CMC, 1 part of potassium fulvate, 10 parts of decomposed organic matters, 5 parts of chlorogenicacid isoreactivity agent, 3.5 parts of steady quality agent.
Be processed as the liquid compound biological soil improver by aforementioned preparation method.
FORMULATION EXAMPLE 2
Component and parts by weight:
40 parts of trichoderma pseudokiningii fermented liquids, 40 parts of fermentation of bacillus subtilis liquid, 1.0 parts of N,O-CMCs, 1 part of potassium fulvate, 10 parts of decomposed organic matters, 4.5 parts in tensio-active agent, 3.5 parts of steady quality agent.
Be processed as the liquid compound biological soil improver by aforementioned preparation method.
FORMULATION EXAMPLE 3
Component and parts by weight:
42 parts of trichoderma pseudokiningii fermented liquids, 38 parts of fermentation of bacillus subtilis liquid, 1.5 parts of N,O-CMCs, 0.5 part of potassium fulvate, 10 parts of decomposed organic matters, 4.5 parts in tensio-active agent, 3.5 parts of steady quality agent;
Be processed as the liquid compound biological soil improver by aforementioned preparation method.
Two. it is following that soil improvement agent of the present invention is used simultaneous test embodiment:
Test example 1
Use FORMULATION EXAMPLE 1 of the present invention, compare, to the influence (Zhaoyuan, Shandong) of Jimai 22 output and the soil weight, pH with single Trichoderma preparation
Test group 1 is for using this product formulation embodiment 1 of 1000ml/ mu; Test group 2 is for using the single Trichoderma preparation of containing of 1000ml/ mu of identical effective spore quantity; Evenly spray before turning in soil surface, control group sprays the equivalent clear water, and the follow-up processing of topdressing is identical; Area 100 ㎡ in sub-district measure behind the harvesting wheat and calculate soil physico-chemical property and wheat yield.The result is as shown in table 5; Descend with respect to the control group soil weight after using this product formulation embodiment 1 and viride bacteria preparation; The pH value is near neutral, and the soil acidification situation obtains reversing, and is suitable for plant growth; Wheat yield increases, and the effect of this product formulation embodiment 1 is superior to single Trichoderma preparation.
Table 5 FORMULATION EXAMPLE 1 of the present invention is compared with the Trichoderma preparation, to the influence of soil physico-chemical property
Test example 2
FORMULATION EXAMPLE of the present invention 2 and existing trichoderma viride, subtilis compound formulation are to the influence (Zhaoyuan, Shandong) of muskmelon soil for growth nutrient
Soil nutrient is the important symbol of soil fertility, is the basis of plant growth.It is Japanese sweet treasured that confession studies thing, and seed is produced by Qingdao Japan sweet precious seed production base.Test group is to get 1000ml/ mu FORMULATION EXAMPLE 2 of the present invention before the field planting to be sprayed on soil surface, and control group is trichoderma viride and a subtilis compound formulation of using identical effective thalline quantity of 1000ml/ mu, turns over.The result is as shown in table 6, and FORMULATION EXAMPLE 2 of the present invention is handled with control group nutrient content in the soil is improved, and has promoted the release of soil nutrient, and the effect of FORMULATION EXAMPLE of the present invention 2 is superior to existing trichoderma viride and subtilis compound formulation.
This product formulation of table 6 embodiment 2 and existing viride, subtilis compound formulation are to the influence of muskmelon output and soil nutrient content
3 product formulation embodiment of test example 2 and existing viride, subtilis compound formulation are to the influence (Zhaoyuan, Shandong) of muskmelon rhizosphere microbe population
The FORMULATION EXAMPLE of the present invention 2 that test group 1 is sprayed 1000ml/ mu for soil surface before the field planting, test group 2 sprays existing trichoderma viride and the subtilis compound formulation that equivalent has identical effective thalline quantity, and control group sprays the equivalent clear water.Mikrobe in the soil not only can be regulated plant strain growth, and has vital role in the maintenance of soil fertility and raising and aspects such as Conversion of energy and material cycle, is the important indicator of soil quality.The result is as shown in table 7; The trichoderma viride of this product formulation embodiment 2 and identical effective thalline quantity and subtilis compound formulation are handled than control group and have all been improved microbe population in the soil; Increased microbial diversity; And the quantity of 2 pairs of bacteriums of this product formulation embodiment and fungi promotes the most remarkable, apparently higher than test group 2.
The influence of table 7 viride, subtilis compound formulation and 2 pairs of muskmelon rhizospheres of this product formulation embodiment microbe population
Test example 4
This product formulation embodiment 2 and commercially available microbial-bacterial fertilizer are to planting the influence (Xinfeng, Jiangxi) of root biomass under time limit Jiangxi south navel orange output and the orcharding in 20 years
Test group 1: require to specifications to use commercially available microbial-bacterial fertilizer (central. set is divided into trace element and active subtilis) 1kg in flower early stage, the every respectively strain of phase of strong fruit; Test group 2: the embodiment of the invention 2 liquid recombiner 100ml are used in every strain; Ditching landfill or water in navel orange plant trunk radius 50cm scope before plant is sprouted respectively; Control group is the equivalent clear water, and the follow-up management mode is identical.After treating fruit harvest, in institute sampling ground, 3 standard navel oranges of picked at random tree is collected 0-45cm soil layer root system, according to the characteristics such as diameter, profile, color, elasticity, Gen Pi and the isolating complexity of center pillar of root press respectively the heavy manual labour root (; 2mm), middle root alive (0.5-2mm), fine work root (<0.5mm), dead four kinds of situation of root tell, and clean repeatedly with zero(ppm) water then, under 85 ℃, dry to constant weight, claim its quality, are scaled unit surface living weight (gm at last
-2).The plant radicula is the most important parts of absorption of nutrients matter in the root system, and their content also is the important indicator of estimating nutrition status of the plant.Can be found out by table 8 and to use commercially available microbial-bacterial fertilizer and this product formulation embodiment 2 radicula content in the navel orange root system that has been significantly increased, dead radical content also significantly descends, and has promoted the growth of the newborn root system of plant, and crop yield improves.The fine work root more can reflect the dietetic alimentation ability of plant as most important component in the root system, and this product formulation embodiment 2 promotes the growth of navel orange fine work root and the raising effect of output to be superior to commercially available microbial-bacterial fertilizer.
This product formulation of table 8 embodiment 2 and of the influence of commercially available microbial-bacterial fertilizer to root biomass and navel orange output under the navel orange orcharding of south, 20 years plantation time limit Jiangxi
Test example 5
This product formulation embodiment 3 and commercially available microbial-bacterial fertilizer and farm manure are to the influence (Zhaoyuan, Shandong) of black fallow soil enzyme activities
Test group 1: use commercially available microbial-bacterial fertilizer 100kg/ mu, test group 2: use this product 1000ml/ mu, control group: applications of farmyard manure (hog manure) 500kg/ mu, turn over after using and rake, measure soil enzyme activities after 6 months.Microbial biomass is closely related in the height of soil enzyme activities and soil physico-chemical property (comprising Soil structure, organic content and soil nutrient content etc.) and the soil, especially with the relation of soil fertility very closely.Sucrase, Phosphoric acid esterase, three kinds of enzyme activities of urase have characterized conversion and the utilization of carbon cycle in the dynamic changes of soil process, soil phosphorus element, the conversion and the utilization of Soil Nitrogen element respectively.Can be found out by table 9, use behind commercially available microbial-bacterial fertilizer, farm manure (hog manure) and this product that the vigor of sucrase, Phosphoric acid esterase and three kinds of enzymes of urase all has lifting in the soil, show that biological activity of soil improves, soil nutrient discharges to be accelerated, and fertility is recovered.The raising of 3 pairs of sucrases of this product formulation embodiment and urease activity is superior to commercially available microbial-bacterial fertilizer and farm manure, and is suitable with commercially available microbial-bacterial fertilizer to the influence of phosphatase activity, is higher than farm manure.
This product formulation of table 9 embodiment 3 and commercially available microbial-bacterial fertilizer and farm manure are to the influence of black fallow soil enzyme activities
Claims (10)
1. compound biological soil improver, its component comprises trichoderma pseudokiningii fermented liquid and fermentation of bacillus subtilis liquid.
2. like the described compound biological soil improver of claim 1, it is characterized in that comprising following main ingredient:
The trichoderma pseudokiningii fermented liquid,
Fermentation of bacillus subtilis liquid,
N,O-CMC,
Potassium fulvate.
3. compound biological soil improver according to claim 1 or claim 2, it is characterized in that wherein the trichoderma pseudokiningii fermented liquid be by trichoderma pseudokiningii (
Trichoderma pseudokiningii) the TRIPI strain fermentation makes, this bacterial classification carries out preservation at China Committee for Culture Collection of Microorganisms common micro-organisms center, and preserving number is CGMCC NO.1443.
4. compound biological soil improver according to claim 1 or claim 2, it is characterized in that wherein fermentation of bacillus subtilis liquid be by subtilis (
Bacillus subtilis) BACI fermentation makes, this bacterial classification carries out preservation at China Committee for Culture Collection of Microorganisms common micro-organisms center, and preserving number is CGMCC NO.1442.
5. compound biological soil improver according to claim 1 or claim 2 is characterized in that the parts by weight of each main ingredient are following:
Trichoderma pseudokiningii fermented liquid 38-42 part, fermentation of bacillus subtilis liquid 38-42 part, N,O-CMC 0.5-1.5 part, potassium fulvate 0.5-1.5 part.
6. compound biological soil improver according to claim 1 or claim 2 is characterized in that adding in the component decomposed organic matter 5-20 weight part.
7. the application of compound biological soil improver according to claim 1 or claim 2; It is characterized in that being used for the improvement and the soil quality that protects the fields---improve soil physico-chemical property, regulate rhizosphere soil microorganism flora structure, promote the growth of root system of plant; Enhance root system is to the receptivity of nutritive ingredient; Improve soil stability and porosity, accelerate precipitation decomposes, and improves Soil structure.
8. the application of compound biological soil improver according to claim 1 or claim 2 is characterized in that being used for the improvement of rice soil, hydromorphic soil, pedocal, alfisol, the multiple soil of saline-alkali soil.
9. the application of compound biological soil improver according to claim 1 or claim 2 is characterized in that muskmelon, wheat, navel orange various crop are had tangible production-increasing function.
10. the application method of compound biological soil improver according to claim 1 or claim 2 is characterized in that with said soil improvement dilution agent 100-200 doubly, before field planting, evenly sprays and turns over behind the soil surface or irritate root; Its usage quantity is respectively 500-2500ml/ mu for looking its soil fertility.
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