CN100334952C - Biologica land biochemical preparation and its preparing method and use - Google Patents
Biologica land biochemical preparation and its preparing method and use Download PDFInfo
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- CN100334952C CN100334952C CNB2005100445275A CN200510044527A CN100334952C CN 100334952 C CN100334952 C CN 100334952C CN B2005100445275 A CNB2005100445275 A CN B2005100445275A CN 200510044527 A CN200510044527 A CN 200510044527A CN 100334952 C CN100334952 C CN 100334952C
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Abstract
The present invention relates to biologic and biochemical preparation and a preparing method thereof. The biologic and biochemical preparation is multiple target site biologic and biochemical preparation which is jointly composed of mixed microorganism comprising Trichoderma pseudokiningii and Bacillus spp, cell membrane protecting materials, composite inductors, natural antibiotic materials comprising trace elements and beneficial elements, such as aminoethylsulfonic acid, light rare earths, Vitamin C, burdock oligosaccharide, chitosan oligosaccharide, dextrin, amino acids, chlorogenic acid, gingko brass, zinc salt, boric acid, ferric salt, etc., wherein the strain of the Trichoderma pseudokiningii which is separated and selected from straws and is named for TRIP1, and the Bacillus spp which is separated from the soil and is named for BAC1, are both stored in the General Microbiological Culture Collection Center (CGMCC) of the China Committee for Culture Collections of Microorganisms. Through production test, the present invention is used for controlling various kinds of diseases for plants, promoting the plant growth, protecting the cell membrane and improving the photochemical efficiency for plants, is particularly suitable for the blight, the greensickness, the downy mildew, the powdery mildew, the grey mold, the nematodiasis, the physiological diseases, red spiders and aphids of cucumbers, tomatoes, the cotton, soybeans and potatoes, and is better than the conventional pesticide. The exemplary in Xushui County in Hebei Province proves that disinfectant cost is reduced from the 400 yuan to 40 yuan per greenhouse through alternately using 1 and 2, and the efficiency is very significant.
Description
Affiliated technical field
The invention belongs to the agricultural chemicals biological technical field, relate to aspect the application two on agricultural of microbial fermentation and microorganism, what be specifically related to is the fermented and cultured of a kind of bacillus and wooden mould spore, mycelia, be used to prevent and treat the plant multiple diseases, promote biology, biochemical pesticide preparation and the compound method thereof of plant growing.
Background technology
Damage by disease and insect is the key factor that reduces crop yield.For the outburst of controlling disease and popular, still depend on the chemosynthesis bactericide basically.This has caused again: (1) environmental pollution, harm person poultry safety; (2) the heavy dose of agricultural chemicals that uses of high frequency has increased production cost; Crop is damaged, reduced quality of agricultural product; (3) use of agricultural antibiotic has also caused the appearance of anti-medicine sudden change microspecies, and new disease is the serious harm crops more; Therefore, press for development environment close friend's disease and pest control agent, exploitation low-residual, nuisanceless, disease and pest control agent efficiently.
1985, only there is a small amount of biological control factor registration to use, account for agricultural chemicals market share less than 1%, nowadays reached more than 30, account for the agricultural chemicals market share 10%.As spraying pseudomonas on the oranges and tangerines surface, the dip-dye site of competition pathogen, control oranges and tangerines penicilliosis; The antibiotic control soil-borne disease that utilizes fluorescent pseudomonas to produce; Utilize the anti-disease fungus cell wall of separating of Serratia secretion chitinase, control pea wilt disease.Utilize the mould fusarium wilt of preventing and treating of wood.1989, domestic having declared with the oil of peel of Citrus reticulata Blanco preserved the patent that oranges and tangerines suppress Penicillium notatum, GINKGO BILOBA EXTRACT analog diseases prevention agent, 1996, induction of resistance agent BTH uses in Germany's registration, the disease that Israel Bar-Ilan university prevents and treats melon crop with the mycelia that produces streptomycin, some different oligosaccharides have been developed a plurality of products as disease-resistant inducer.
In recent years, the anti-preparation of the crop disease and insect of many targets also has certain development.The long preparation of some plant growth-promotings all shows certain prophylaxis effect in the field.Patent 01123508.x CN1328769A " a kind of agriculturally useful compositions that contains water soluble chitosan ", patent 01124042.3 CN1180693C " a kind of plant growth regualting composition ", patent 03111946.8 CN1435406A " a kind of method for preparing chlorogenic acid ", patent 200410036582.5 CN1634640A " pesticidal applications of actinidia resource ", these patents mainly also are based on the one-side control of cause of disease the measure of disease control, or only be disease-resistant factor of inducing this single (host), effect is limited.
Summary of the invention
The purpose of this invention is to provide a kind of on existing biological and ecological methods to prevent plant disease, pests, and erosion corps diseases basis the preparation at different target position produce synergistic effect in optimum concentration; selection has the material of collaborative or synergistic effect; send and lure material anti-, growth-promoting functions; this fermentative microorganism and protection cell membrane material; co-induction; natural antimicrobial substance, common many target position of forming such as trace element and beneficial element are biochemistry-biopesticide preparation and compound method thereof efficiently.
The objective of the invention is to realize by following technical scheme.
At first technology and the effect according to existing patent can draw: (1) host has resistance to causal organism itself, and this kind resistance can be induced; (2) host has only specific tricky time to pathogen; (3) some organic nutrient substance and simple molecules such as amino acid, glucose, oxalic acid etc. can be removed the harm of pathogen to the host; (4) some inorganic nutrients can improve disease resistance of plant; (5) beneficial microbe can be competed the ecological niche of pathogen.Only the preparation at different target position just can produce synergistic effect in optimum concentration; Therefore, change unilateral control strategy, select to have the material of collaborative or synergistic effect, can develop and lure biochemistry-biologic product efficiently anti-, growth-promoting functions.
By above conclusion, the present inventor thinks that only the preparation at different target position just can produce synergistic effect in optimal concentration, specifically establishes the juice scheme and is: (1) carries out the body-building cultivation with aggregate measures, improve the basis of crop disease-resistant, its key is the protection cell membrane system; (2) with dissimilar secondary metabolites and inhibition or kill pathogen; (3) carry out disease-resistant induce etc. with comprehensive means; (4) with beneficial microbe competition pathogen ecological niche.
The present invention adopts a kind of mixed microorganism that contains a strain trichoderma pseudokiningii (Trichoderma pseudokiningii) and a bacillus (Bacillus spp); with protection cell membrane material; co-induction; natural antimicrobial substance, common many target position biology--the biochemical preparation of forming of trace element and beneficial element.Be used to prevent and treat the plant multiple diseases, promote plant growing.
Biology of the present invention, biochemical pesticide preparation are formulated by the raw material of following (part) by weight ratio:
The better scope optimum range of raw material
Trichoderma pseudokiningii zymotic fluid 20-40 25-40
Fermentation of bacillus liquid 30-50 35-45
Composition A 2.5-5.2 3-5
Composition B 5-15 10-15
Composition C 10-15 10-15
Components D 15-26 17-25
Wherein:
Trichoderma pseudokiningii zymotic fluid: contain Trichoderma silk dry weight 0.1-1g in every 100ml zymotic fluid;
Fermentation of bacillus liquid: bacillus 1 * 10 in every milliliter of fermentation of bacillus liquid
7-1 * 10
10Individual;
Contain following raw material (part) composition by weight among the mentioned component A: (containing in 100 grams)
Taurine 20-35 20-30
Light rare earth 20-50 30-45
Vitamin C 5-11 5-10
Containing following material among the composition B forms: (containing in 100 grams)
Burdock oligosaccharide 20-40 25-35
Chitosan oligosaccharide 15-25 17-25
Dextrin 25-40 30-40
Amino acid/11 0-20 11-18
Containing following material among the composition C forms: (containing in 100 grams):
Chlorogenic acid 40-70 45-60
Ginkgo brass 35-55 40-50
Containing following material in the components D forms: (containing in 100 grams)
Zinc salt 25-40 25-35
Boric acid 15-40 25-35
Molysite 15-50 27-43
Above-mentioned many target position biochemistry-biopesticide preparing preparation efficiently are:
1. the preparation method of trichoderma pseudokiningii fermentation: answer sterile working in the processing; At first the bacterial classification in the mould test tube of wood is added in the fungi culture medium, cultivate on shaking table the sterilization back, get seed liquor, put in the seeding tank again and cultivate, constantly stir, enter the secondary seed jar again and stir, inject the fermentation tank stirring and evenly mixing then, enter the high speed shear machined again, enter the economic benefits and social benefits inspissator again and under 45 ℃ temperature, concentrate and make wooden mould culture;
2. fermentation of bacillus subtilis preparation method: answer sterile working in the processing; The bacillus subtilis test tube strains is added in the bacteria culture media, on shaking table, cultivate, get seed liquor, put in the seeding tank again and cultivate, constantly stir, enter the secondary seed jar again and stir, inject the fermentation tank stirring and evenly mixing then, enter emulsification in the emulsion tank again, enter economic benefits and social benefits inspissator concentrated bacillus subtilis culture that makes under 45 ℃ temperature again;
3. respectively composition A, B, C in the raw material are dissolved at normal temperatures, be modulated into behind the cream standby again; Again with boiling together after the components D dissolving, then with composition A, the B, C, the D mixing for standby use that modulate;
4. last with above-mentioned standby trichoderma pseudokiningii culture, bacillus subtilis culture and composition A, B, C, the merging in material-compound tank of D mixture, mixing, emulsification, qualifiedly after testing again be finished product of the present invention.
Described 4 kinds of composition A, B, C, D are bioactivator or biological necessary micro-first rope, and the element that trace element can replenish crop self lacks, and to a certain extent, can protect its cell membrane, improves crop quality, improves crop resistance; Biological active matter mass-energy effectively suppresses or pathogenic microbe killing, and makes the deposits yields protective plant protecting agent by inducing, and improves self resistance, promotes the crop healthy growth.
Effect of the present invention is very obvious, because the present invention is the mixed microorganism that adopts a strain trichoderma pseudokiningii (Trichodermapseudokiningii) and a bacillus (Bacillus spp), and added protection cell membrane material, co-induction, natural antimicrobial substance, composition A, the B of trace element and beneficial element, common many target position biology--the biochemical preparation of forming of C, D; Through production test, control cucumber, tomato, eggplant, cotton, soybean, sweet potato disease are had good prevention and therapeutic action, effective to fusarium wilt, verticillium wilt, downy mildew, powdery mildew, gray mold, nematodosis and physiological disturbance and two spotted spider mite and aphid; Can control the generation of above-mentioned disease, control efficiency is better than conventional pesticide; Demonstration in Xushui, Hebei proves, is used alternatingly this medicine, and each booth bactericide input is reduced to 40 yuan, the benefit highly significant by 400 yuan.
Description of drawings
Fig. 1 is a raw material trichoderma pseudokiningii zymotechnique flow chart of the present invention
Fig. 2 is a raw material Bacillus subtilis zymotechnique flow chart of the present invention
Fig. 3 is a preparation production technique flow chart of the present invention
Embodiment
As shown in Figure 1, the preparation method of trichoderma pseudokiningii fermentation: answer sterile working in the processing; At first the trichoderma pseudokiningii bacterial classification is activated 24h through the PDA flat board, after receive in the PD liquid nutrient medium triangular flask, 28 ℃, 100r/min cultivates 36h, makes seed liquor, receive in the seeding tank by 3% inoculum concentration, cultivate and stir aerobic culture 36h, enter the secondary seed jar and stir aerobic culture 36h, inject fermentation tank then and stir aerobic culture 96h, enter high speed shears mixing, embedding, enter the economic benefits and social benefits farming device that contracts again and under 45 ℃ temperature, concentrate and make the trichoderma pseudokiningii culture.
Fermentation of bacillus subtilis preparation method as seen from Figure 2: answer sterile working in the processing; The bacillus subtilis test tube strains is activated through the NA culture medium flat plate, receive and on shaking table, cultivate 24h in the NA liquid nutrient medium, inoculum concentration by 3% carries out stirring in the seeding tank aerobic culture 24h, enter the secondary seed jar again and stir aerobic culture 24h, inject fermentation tank then and stir aerobic culture 48h, enter emulsification embedding in the emulsion tank again, enter economic benefits and social benefits inspissator concentrated bacillus subtilis culture that makes under 45 ℃ temperature again.
Fig. 3 is the technological process of production figure of biology of the present invention, biochemical pesticide preparation, and bacillus subtilis culture that will make earlier and trichoderma pseudokiningii culture merge and add mixing in the material-compound tank; Add in the material-compound tank after again composition A, B, C being dissolved, transfer cream respectively; Again components D dissolving, the back that boils together are added in the material-compound tank; In material-compound tank the raw material added is mixed then, again through emulsification, it is standby to carry out finished product packing warehouse-in after qualified after testing.
Foregoing 4 kinds of composition A, B, C, D are bioactivator or biological necessary micro-first rope, and the element that trace element can replenish crop self lacks, and to a certain extent, can protect cell membrane, improves crop quality, improves crop resistance; Biological active matter mass-energy effectively suppresses or pathogenic microbe killing, and makes the deposits yields protective plant protecting agent by inducing, and improves self resistance, promotes the crop healthy growth.
Trichoderma strain among the present invention is trichoderma pseudokiningii (Trichoderma pseudokiningii) bacterial strain, separation screening obtains from Jinan City's Licheng District maize straw, called after TRIP1 has been kept at China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC); Activate bacterial strain with solid potato culture medium, potato bran mass (wheat bran 40g/L, potato 50g/L, sucrose 20g/L, KH
2PO
40.5g/L, MgSO
47H
2O 0.5g/L, CaCl
20.25g/L, pH 5-7,28 ℃ of cultivation temperature, rotating speed 20-50RPM, inoculum concentration 3-7%), trichoderma pseudokiningii can reach in the incubation time at 36 hours and contain Trichoderma silk dry weight 0.1-1g in the 100mL zymotic fluid; Trichoderma pseudokiningii bacteria liquid medium is potato, bran mass.
Medium contains PDA, and the bacterial classification of liquid culture activation 36 hours gets trichoderma pseudokiningii spore and hypha fermentation liquid.
Trichoderma pseudokiningii of the present invention also can carry out solid fermentation with solid culture medium, medium is 7: 3 powder of straw and a wheat bran, other adds 4% ammonium sulfate, 0.4% potassium dihydrogen phosphate, 0.1% magnesium sulfate is optimal medium, 28-32 ℃ is suitable cultivation temperature, 30 ℃ is optimum temperature, 4% is optimum inoculation amount, and 96h arrives the fermentation peak.Produce spore as raw material.
The present invention separates from the vegetable soil of Shouguang, Shandong and obtains a bacillus (Bacillus spp), and called after BAC1, bacterial classification are kept at China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC); Carry out liquid fermentation, medium is tryptone, yeast extract, NaCl, and liquid culture reached 3,000,000,000/ML in 48 hours, also can directly use the obtaining liq Mixed Microbes.
The material of one group of protection cell membrane that the present invention uses is taurine, light rare earth, ascorbic composition or any composition--A
The disease-resistant elicitor that the present invention uses is a burdock oligosaccharide, chitosan oligosaccharide, dextrin, amino acid and composition thereof, composition--B
The natural antimicrobial substance that the present invention uses is a chlorogenic acid, GINKGO BILOBA EXTRACT and composition thereof, composition--C
The trace element and the beneficial element of the plant growing that the present invention uses are zinc, boron, iron, composition--D
(1: 3-3: 1) mix, the solid product I of preparation or fluid product II add composition A then according to a certain percentage, B, C, D, preparation liquid and solid product in proportion with BAC1 with TRIP1 in the present invention; With also playing good preventive and therapeutic effect behind the bacteria inactivation rate in the sample preparation.
Biology of the present invention, biochemical preparation specific embodiment are as follows:
Example 1, biology of the present invention, biochemical preparation are formulated by the raw material of following (part) by weight ratio:
Trichoderma pseudokiningii zymotic fluid 35, fermentation of bacillus liquid 40, composition A 4, composition B 9, composition C 12, components D 20; Wherein composition A is formulated by the raw material of following (part) by weight ratio: taurine 30, light rare earth 35, vitamin C 8; Composition B is formulated by the raw material of following (part) by weight ratio: burdock oligosaccharide 30, chitosan oligosaccharide 20, dextrin 32, amino acid/11 5; Composition C is formulated by the raw material of following (part) by weight ratio: chlorogenic acid 55, ginkgo brass 45; Components D is formulated by the raw material of following (part) by weight ratio: zinc salt 30, boric acid 27, molysite 35.
Example 2, biology of the present invention, biochemical preparation are formulated by the raw material of following (part) by weight ratio: trichoderma pseudokiningii zymotic fluid 30, fermentation of bacillus liquid 35, composition A 3, composition B 7, composition C10, components D 15; Wherein composition A is formulated by the raw material of following (part) by weight ratio: taurine 25, light rare earth 25, vitamin C 5; Composition B is formulated by the raw material of following (part) by weight ratio: burdock oligosaccharide 23, chitosan oligosaccharide 17, dextrin 27, amino acid/11 2; Composition C is formulated by the raw material of following (part) by weight ratio: chlorogenic acid 42, ginkgo brass 35; Components D is formulated by the raw material of following (part) by weight ratio: zinc salt 27, boric acid 18, molysite 20.
Example 3, biology of the present invention, biochemical preparation are formulated by the raw material of following (part) by weight ratio: trichoderma pseudokiningii zymotic fluid 40, fermentation of bacillus liquid 45, composition A 5, composition B 13, composition C 15, components D 25; Wherein composition A is formulated by the raw material of following (part) by weight ratio: taurine 35, light rare earth 45, vitamin C 11; Composition B is formulated by the raw material of following (part) by weight ratio: burdock oligosaccharide 40, chitosan oligosaccharide 25, dextrin 37, amino acid/11 9; Composition C is formulated by the raw material of following (part) by weight ratio: chlorogenic acid 66, ginkgo brass 53; Components D is formulated by the raw material of following (part) by weight ratio: zinc salt 38, boric acid 36, molysite 46.
Concrete using method can spray and irritate root, 7-10 days blanking time, can be used alternatingly with conventional pesticide.
With concrete experiment embodiment positive effect of the present invention is described below:
Example 1: the inoculation cucumber downy mildew, the present invention is to the results from pot experiment test (Xinjiang) of protection purpleflower violet herb downy mildew
| Preparation | Morbidity numbers at different levels (mean values of three repetitions) | Disease index (%) | Control efficiency (%) | ||||
| 0 grade | 1 grade | 2 grades | 3 grades | 4 grades | |||
| I II I II CK | 9 20 11 17 0 | 18 7 10 8 0 | 0 0 6 1 12 | 0 0 0 1 10 | 0 0 0 0 5 | 16.8 6.4 20.4 12.0 68.5 | 75.4 90.7 70.2 82.5 0 |
Example 2: the present invention is to the control efficiency (Qingdao City is China ink both) of plastic tent cucumber downy mildew
| The investigation date | I30 doubly | I50 doubly | II50 doubly | II100 doubly | The Sandofan disease refers to | ||||
| Disease refers to | Preventive effect | Disease refers to | Preventive effect | Disease refers to | Preventive effect | Disease refers to | Preventive effect | ||
| November 12 November 04 October 22 October 09 | 16.8 12.7 15.8 24.0 | 62.1 53.6 34.4 34.4 | 21.9 12.2 13.3 22.4 | 50.6 55.5 44.8 38.8 | 37.7 19.5 9.1 17.5 | 14.9 28.8 62.2 52.2 | 38.2 26.2 11.2 18.1 | 13.8 4.3 53.5 50.5 | 44.3 27.4 24.1 36.6 |
Example 3: the present invention is to the control efficiency of protection purpleflower violet herb powdery mildew
| Handle | November 12 | November 25 | ||||||||
| Disease refers to | Morbidity strain number | Total investigation strain | The incidence of disease | Preventive effect | Disease refers to | Morbidity strain number | Total investigation strain | The incidence of disease | Preventive effect | |
| I30 times I50 times I100 times II50 times II100 times II150 times of Sandofan | 0 0 0 0 1.9 1.9 4.0 | 0 0 0 0 3 2 6 | 34 34 34 34 34 34 34 | 0 0 0 0 8.8 5.9 17.6 | 100 100 100 100 52.5 52.5 | 0 0 1.9 0 2.2 2.3 5.9 | 0 0 2 0 5 6 8 | 34 34 34 34 34 34 34 | 0 0 5.9 0 14.7 17.6 23.5 | 100 100 67.8 100 62.7 61.0 |
Example 4: the present invention withers+the concurrent control efficiency of grey mold to tomato yellow; After the verticillium wilt morbidity, other agricultural chemicals is invalid, and II irritates root once, and investigation on December 18 disease refers to that verticillium wilt and gray mold are respectively contrast 76,43 processing and are kept to 28 and 14.
Example 5: the present invention is to capsicum downy mildew, the control efficiency that powdery mildew is concurrent, I50 doubly, II50,100 times of relative control effects reach 68,80,59% respectively.
Example 6: the protective effect of cell membrane of the present invention.The blade of wheat the 1st leaf position of each processing is cut into the long fragment of 1cm, uses the clear water rinsing, use distilled water flushing again 3 times, with clean gauze suck dry moisture, fully mixing takes by weighing the blade 0.5g that each is handled respectively, places 15ml tool plug scale test tube, slowly add 10ml distilled water, blade is immersed in the water fully, vibration, and with the vavuum pump 5min that bleeds, in 25 ℃ of waters bath with thermostatic control, leave standstill 30min, measure the electrical conductivity of the outer sepage of tissue with DDS-11A type conductivity gauge (Shanghai thunder magnetic instrument plant).Place boiling water bath to boil 15min this solution again, its electrical conductivity is measured in the cooling back.Calculate its relative conductivity.The experiment triplicate.
Table: preparation of the present invention is to the protective effect of cucumber cotyledons cell membrane
| Handle | Saturating relatively property (%) |
| CK 1∶20000 1∶50000 1∶80000 1∶100000 | 15.1 7.2 6.2 5.8 5.6 |
Example 7: the present invention is to the raising effect of plant photochemistry efficient.The luminous energy that photosynthetic mechanism absorbs has three possible whereabouts: the one, be used to promote photochemical reaction, and the separation of charge at the center that induces reaction and afterwards electronics transmission and photophosphorylation are formed for fixing, the assimilatory power (ATP and NADPH) of reducing carbon dioxide; The 2nd, be transformed into heat leakage; The 3rd, emit with the form of fluorescence.Owing to have this those long relations of vying each other that disappear between this three, so can be by the photosynthetic variation of change detection of fluorescence.Because can measure the fluorescence of intact leaf, chlorophyll a fluorescence induction dynamics can become the fabulous probe of in situ detection photosynthesis PS II reaction center important step.Modulation system fluorescence is an important tool measuring PS II photochemistry efficient (Fv/Fm).Fv/Fm-does not suffer environment-stress and through abundant scotopic plant leaf blade PS II quantum efficiency index maximum or potential, Fv/Fm is also referred to as the energy capture efficient of open PS II reaction center, the photochemistry efficient of promptly potential PS II sometimes.Light is suppressed, and photosynthesis is poor, always causes the reduction of Fv/Fm, and vice versa.Therefore the variation of Fv/Fm can be as the diagnosis index of photosynthesis power.Spray and one week of back measure photochemistry efficient with plant efficient instrument.Following data show that the present invention has obvious facilitation to several plant photochemistry efficient.
| Plant | Processed group (Fv/Fm) X ± Sd | Control group (Fv/Fm) X ± Sd | Photochemistry efficient raising rate (%) |
| Moth orchid gardenia Jasmine | 0.8108±0.01977 0.8076±0.00607 0.8044±0.00483 | 0.7286±0.01403 0.7142±0.05309 0.7676±0.0290 | 11.28 13.08 4.794 |
P＜0.05, significant difference
| Cotton | Processed group (Fv/Fm) X ± Sd | Control group (Fv/Fm) X ± Sd | Photochemistry efficient raising rate (%) |
| Upper blade middle part blade lower blade | 0.7478±0.01949 0.7982±0.02262 0.8350±0.01049 | 0.6560±0.03639 0.7792±0.01805 0.7496±0.0208 | 13.99 2.438 11.39 |
P＜0.1, significant difference
| Cotton | Processed group (Fv/Fm) X ± Sd | Control group (Fv/Fm) X ± Sd | Photochemistry efficient raising rate (%) |
| Upper blade middle part blade lower blade | 0.7824±0.01496 0.8288±0.00638 0.8324±0.00365 | 0.6948±0.02382 0.8044±0.01106 0.7950±0.01994 | 12.61 3.033 4.704 |
P＜0.1, significant difference
| Grape | Processed group (Fv/Fm) X ± Sd | Control group (Fv/Fm) X ± Sd | Photochemistry efficient raising rate (%) |
| Upper blade middle part blade lower blade | 0.7430±0.00561 0.7832±0.0092 0.7658±0.01331 | 0.6958±0.01385 0.7304±0.00635 0.7382±0.0061 | 6.784 7.229 3.739 |
| Soybean | Processed group (Fv/Fm) X ± Sd | Control group (Fv/Fm) X ± Sd | Photochemistry efficient raising rate (%) |
| Upper blade middle part blade lower blade | 0.8158±0.01006 0.8296±0.00336 0.8136±0.00493 | 0.7712±0.01033 0.7804±0.00709 0.7698±0.00811 | 5.783 6.304 5.690 |
| Peanut | Processed group (Fv/Fm) X ± Sd | Control group (Fv/Fm) X ± Sd | Photochemistry efficient raising rate (%) |
| Middle part blade lower blade | 0.8068±0.00512 0.8286±0.0068 | 0.7662±0.00691 0.7938±0.00249 | 5.299 4.384 |
| Chinese yam | Processed group (Fv/Fm) X ± Sd | Control group (Fv/Fm) X ± Sd | Photochemistry efficient raising rate (%) |
| Upper blade middle part blade lower blade | 0.7946±0.0077 0.8230±0.00436 0.7914±0.01074 | 0.7630±0.0097 0.7906±0.0106 0.7608±0.00701 | 4.142 4.098 4.022 |
The present invention's " biological, biochemical preparation and compound method and purposes " is involved needs the microorganism fungus kind preservation: trichoderma pseudokiningii bacterial strain and bacillus subtilis, in application on August 22nd, 2005 and by " China Committee for Culture Collection of Microorganisms's common micro-organisms " center " collection; the collection address is the No. 13, North No.1 Row, Zhongguancun, Haidian District, Beijing City, Institute of Microorganism, Academia Sinica.The classification name of this center suggestion is respectively trichoderma pseudokiningii (Trichodermapseudokoningii) bacterial strain, and register on the books and be numbered in the preservation center: CGMCCNo.
1443Bacillus subtilis (Bacillus subtilis) bacterial strain, register on the books and be numbered in the preservation center: CGMCCNo.
1442
Claims (4)
- " a kind of biology, biochemical preparation; it is characterized in that: it is the mixed microorganism that contains trichoderma pseudokiningii (Trichode rma pseudokiningii) and bacillus subtilis (Bacillussubtilis); and with protection cell membrane material-taurine, light rare earth, ascorbic composition; disease-resistant elicitor-burdock oligosaccharide, chitosan oligosaccharide, dextrin, amino acid whose composition; the composition of natural antimicrobial substance-chlorogenic acid, GINKGO BILOBA EXTRACT, many target position biology--the biochemical preparation of the common composition of the composition of trace element and beneficial element-zinc, boron, iron; Trichoderma pseudokiningii be from separation screening on the stalk to bacterial strain, preserving number is: CGMCC No.1443; Bacillus subtilis is the bacillus subtilis that is separated to from soil, and preserving number is: CGMCCNo.1442; Trichoderma pseudokiningii is to activate bacterial strain with solid potato culture medium, the potato bran mass, and liquid culture activation 36 hours makes the trichoderma pseudokiningii zymotic fluid, and its concentration is: Trichoderma silk dry weight 0.1～1g/100ml zymotic fluid; Bacillus subtilis is to be medium with tryptone, yeast extract, NaCl, and liquid culture 48 hours makes fermentation of bacillus subtilis liquid, and its concentration is: 1 * 10 7～1 * 10 10Individual bacillus/ml zymotic fluid; The parts by weight of each composition are in the preparation: trichoderma pseudokiningii zymotic fluid 20-40; fermentation of bacillus subtilis liquid 30-50; protection cell membrane material 2.5-5.2; disease-resistant elicitor 5-15; natural antimicrobial substance 10-15; trace element and beneficial element 15-26; wherein; parts by weight in the protection cell membrane material between each raw material are taurine 20-35; light rare earth 20-50; vitamin C 5-11; parts by weight in the disease-resistant elicitor between each raw material are burdock oligosaccharide 20-40; chitosan oligosaccharide 15-25; dextrin 25-40; amino acid/11 0-20; parts by weight in the natural antimicrobial substance between each raw material are chlorogenic acid 40-70; GINKGO BILOBA EXTRACT 35-55, the parts by weight in trace element and the beneficial element between each raw material are zinc salt 25-40; boric acid 15-40; molysite 15-50.
- 2. a kind of biology as claimed in claim 1; biochemical preparation; it is characterized in that: the parts by weight of each composition are in the preparation: trichoderma pseudokiningii zymotic fluid 25-40; fermentation of bacillus subtilis liquid 35-45; protection cell membrane material 3-5; disease-resistant elicitor 10-15; natural antimicrobial substance 10-15; trace element and beneficial element 17-25; wherein; parts by weight in the protection cell membrane material between each raw material are taurine 20-30; light rare earth 30-45; vitamin C 5-10; parts by weight in the disease-resistant elicitor between each raw material are burdock oligosaccharide 25-35; chitosan oligosaccharide 17-25; dextrin 30-40; amino acid/11 1-18; parts by weight in the natural antimicrobial substance between each raw material are chlorogenic acid 45-60; GINKGO BILOBA EXTRACT 40-50, the parts by weight in trace element and the beneficial element between each raw material are zinc salt 25-35; boric acid 25-35; molysite 27-43.
- 3. a kind of biology as claimed in claim 1; biochemical preparation; it is characterized in that: the parts by weight of each composition are in the preparation: trichoderma pseudokiningii zymotic fluid 35; fermentation of bacillus subtilis liquid 40; protection cell membrane material 4; disease-resistant elicitor 9; natural antimicrobial substance 12; trace element and beneficial element 20; wherein; parts by weight in the protection cell membrane material between each raw material are taurine 30; light rare earth 35; vitamin C 8; parts by weight in the disease-resistant elicitor between each raw material are burdock oligosaccharide 30; chitosan oligosaccharide 20; dextrin 32; amino acid/11 5; parts by weight in the natural antimicrobial substance between each raw material are chlorogenic acid 55; GINKGO BILOBA EXTRACT 45, the parts by weight in trace element and the beneficial element between each raw material are zinc salt 30; boric acid 27; molysite 35.
- 4. the purposes of a kind of biology as claimed in claim 1, biochemical preparation is characterized in that: be used to prevent and treat cucumber downy mildew, powdery mildew, tomato verticillium wilt, gray mold, capsicum downy mildew, powdery mildew.
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