CN102342300A - Composition comprising brevibacillus brevis TW and trichodermin - Google Patents
Composition comprising brevibacillus brevis TW and trichodermin Download PDFInfo
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- CN102342300A CN102342300A CN2011102194154A CN201110219415A CN102342300A CN 102342300 A CN102342300 A CN 102342300A CN 2011102194154 A CN2011102194154 A CN 2011102194154A CN 201110219415 A CN201110219415 A CN 201110219415A CN 102342300 A CN102342300 A CN 102342300A
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Abstract
The invention belongs to the technical field of farm chemicals, and relates to a composition comprising brevibacillus brevis TW and trichodermin for preventing soil-borne diseases of melons, fruits and vegetables. The composition is wettable powder, which comprises 1 billion to 100 billion CFU/g of brevibacillus brevis TW and 10 million to 10 billion CFU/g of trichodermin; and the composition also comprises other ingredients in percentage by weight such as: 1 to 40 percent of dispersant, 1 to 40 percent of wetting agent and the balance of filling materials. The composition has the advantages that: (1) a good effect on preventing verticillium wilt and root rot of strawberries and blight and damping-off of vegetables can be realized; (2) the lasting period for the verticillium wilt and root rot of strawberries and the blight and damping-off of vegetables is long, so the times of the drug application can be reduced, and the prevention cost can be reduced; and (3) the composition is micro-organism combined preparation, has no chemical residue and is safe to use.
Description
Technical field
The invention belongs to technical field of pesticide, relate to a kind of composition that contains short bacillus pumilus TW and Trichoderma that can be used for preventing and treating the soil-borne disease of melon and fruit, vegetables.
Background technology
Along with improving constantly of facility canopy chamber melon and fruit, vegetable continuous cropping, cropping index, the generation of soil-borne disease is also serious gradually, becomes the outstanding problem of restriction facility fruits and vegetables yield and quality.Therefore, resolve melon and fruit, the soil-borne diseases of vegetable problem of preventing and treating seems more and more important.Melon and fruit, soil-borne diseases of vegetable are meant that pathogene lives in the soil, and under the suitable situation of condition, pathogene infects from its root or stem foot; In the morbidity later stage, can cause the plant mortality, cause melon and fruit, the vegetables underproduction; Influence benefit and improve, belong to destructive disease.Harm strawberry, more serious the having of vegetables: fusarium wilt, verticillium wilt, damping off, root rot, damping off etc.
Short bacillus pumilus TW (Latin generic name: Brevibacillus brevis); It is the novel bacterial of Zhenjiang, knob, Jiangsu research of agricultural science exploitation; Preserving number is: CCTCC NO:M207014; Its morphological feature is that bacterium is direct rod shape; Tool nose circle or square end, colonial morphology is round, protuberance; The edge is smooth, yellow-white to milky bacterium colony; Physiological and biochemical property is Gram-positive, and is aerobic, and peritrichous can produce gemma, gemma ellipse or column; The fermentation fluid power of short bacillus pumilus produces multiple antibacterial peptide; Fermentation fluid power antagonism rice blast fungus, Rhizoctonia solani Kuhn, the sick fungi of various plants such as rhizoctonia cerealis and Sclerotinia sclerotiorum.Short bacillus pumilus has prominent features such as growth is fast, nutrition is simple and it is heat-resisting to produce, degeneration-resistant gemma; Producing, formulation processing and the survival in environment, growing and breeding surely and make it not only help biocontrol fungicide; And mass production processes is simple; Cost is lower; Use conveniently; Storage life is long, is a kind of desirable biocontrol bacteria.
Trichoderma is the thread imperfect fungus that one type of occurring in nature extensively exists.Its member can both find in various types of soil, comprises forest, farmland and orchard.Seldom relevant for the report of on live plant, finding Trichoderma, and less than relevant report about Trichoderma endoparasitism live plant.Trichoderma is the jinx of soil-borne fungus pathogen.Its mode of action is various, comprises chemical action, antibiosis and parasitization etc.Parasitization is similar to the chemotactic growth, and the mycelia of Trichoderma is sensed the agglutinin of host's (phytopathogen) secretion and tends to host's (phytopathogen) growth.The agglutinin of host's (phytopathogen) secretion can combine the galactose residue on the Trichoderma cell wall, and Trichoderma begins to invade host's (phytopathogen) through this position.Trichoderma can secrete a series of cell wall degrading enzyme and antibiotic.Cell wall degrading enzymes include ?
-1,3 - glucanase, chitinase and protease.In addition, existingly report that Trichoderma can produce multiple volatile or nonvolatile antibiotic.It is generally acknowledged that these enzymes and antibiotic acting in conjunction are in the host: cell wall degrading enzyme is disintegrated host's cell wall earlier, increased the probability that antibiotic diffuses into host cell simultaneously.Through the physics contact, the mycelia of Trichoderma is wrapped on the host, invades host's growth inside thereupon, and very fast host will reduce and disintegration because of the turgor pressure of cell.
Summary of the invention
Technical problem to be solved by this invention is that a kind of good composition that contains short bacillus pumilus TW and Trichoderma of soil-borne disease control efficiency to control melon and fruit, vegetables is provided.
The composition that contains short bacillus pumilus TW and Trichoderma of the present invention is a wetting powder, contains short bacillus pumilus TW10 hundred million-1,000 hundred million CFU/ gram, Trichoderma 0.1 hundred million-10,000,000,000 CFU/ gram in its preparation; Other compositions of preparation and percentage by weight are dispersant 1%-40%, wetting agent 1%-40%, filler surplus.
Said dispersant can be the combination of any one or two kinds in sodium lignin sulfonate, neopelex, MF, NNO, CNF and the dispersant known in those skilled in the art.
Said wetting agent can be that sodium butylnaphthalenesulfonate, succinic acid Arrcostab sodium sulfonate, JFC, tea are withered, the combination of any one or two kinds in spaonin powder, excrementum bombycis meal, washing powder and the wetting agent known in those skilled in the art.
Said filler can be the combination of any one or two kinds in kaolin, talcum powder, calcium carbonate, potter's clay, diatomite, bentonite, white carbon, pyrophyllite, attapulgite and the filler known in those skilled in the art.
Can make wetting powder of the present invention according to the method for well known to a person skilled in the art; Subsequent use after promptly wetting agent, dispersant and filler being pulverized with airslide disintegrating mill; Mix with short bacillus pumilus TW conidial powder, Trichoderma conidial powder and wetting agent, dispersant, filler; In mixer, mix, promptly get product of the present invention.
Advantage of the present invention and good effect: (1) is to strawberry verticillium wilt, root rot; Vegetables damping off and damping off have higher control efficiency; As 10,000,000,000 CFU/ restrain short bacillus pumilus TW trichoderma wettable powder to strawberry verticillium wilt, root rot medicine after 30 days preventive effects reach 64.5% and 72.4% respectively; 30 days preventive effects behind the medicine of tomato damping off and damping off are reached 78.6% and 76.5% respectively, but also can promote the growth of vegetables in seedling stage; (2) since short bacillus pumilus TW and Trichoderma built wettable powder to strawberry verticillium wilt, root rot, the lasting period of vegetables damping off, damping off is long, so can reduce the medication number of times, reduction prevents and treats cost; (3) these article are the microorganism combination preparation, do not have chemical residual, and are safe in utilization.
Embodiment
Embodiment 1
Prescription (each constituent and percentage by weight): 1,000 hundred million CFU/ restrain short bacillus pumilus TW6%, 10,000,000,000 CFU/ gram Trichoderma 40%, sodium lignin sulfonate 10%, sodium butylnaphthalenesulfonate 10%, diatomite 34%.
Preparation: it is even that short bacillus pumilus TW, Trichoderma, sodium lignin sulfonate, sodium butylnaphthalenesulfonate, diatomite are added in the agravic agitator premix; Add again in the inlet air flow cracker; Control is pulverized temperature below 10 ℃, pulverizes by 325 order fineness, and pulverizing is subsequent use after accomplishing; The short bacillus pumilus TW, Trichoderma and the auxiliary agent that crush are added in the mixer again, fully mix, obtain finely disseminated 10,000,000,000 CFU/ and restrain short bacillus pumilus TW trichoderma wettable powder.
Embodiment 2
((each constituent and percentage by weight): 1,000 hundred million CFU/ restrain short bacillus pumilus TW15% to prescription, 10,000,000,000 CFU/ restrain Trichoderma 50%, sodium lignin sulfonate 5%, NNO5%, sodium butylnaphthalenesulfonate 10%, kaolin 10%, bentonite 5%.
Preparation: it is even that short bacillus pumilus TW, Trichoderma, sodium lignin sulfonate, NNO, sodium butylnaphthalenesulfonate, kaolin, bentonite are added in the agravic agitator premix; Add again in the inlet air flow cracker; Control is pulverized temperature below 10 ℃; Pulverize by 325 order fineness, pulverizing is subsequent use after accomplishing; The short bacillus pumilus TW, Trichoderma and the auxiliary agent that crush are added in the mixer again, fully mix, obtain finely disseminated 20,000,000,000 CFU/ and restrain short bacillus pumilus TW trichoderma wettable powder.
Embodiment 3
Prescription (each constituent and percentage by weight): 1,000 hundred million CFU/ restrain short bacillus pumilus TW36%, 10,000,000,000 CFU/ gram Trichoderma 40%, MF5%, JFC5%, white carbon 5%, bentonite 9%.
Preparation: it is even that short bacillus pumilus TW, Trichoderma, MF, JFC, white carbon, bentonite are added in the agravic agitator premix; Add again in the inlet air flow cracker; Control is pulverized temperature below 10 ℃; Pulverize by 325 order fineness; Pulverizing is subsequent use after accomplishing, and obtains finely disseminated 40,000,000,000 CFU/ and restrains short bacillus pumilus TW trichoderma wettable powder.
Claims (4)
1. composition that contains short bacillus pumilus TW and Trichoderma, it is characterized in that: its preparation is a wetting powder, contains short bacillus pumilus TW10 hundred million-1,000 hundred million CFU/ gram, Trichoderma 0.1 hundred million-10,000,000,000 CFU/ gram in its preparation; Other compositions of preparation and percentage by weight are dispersant 1%-40%, wetting agent 1%-40%, filler surplus.
2. the composition that contains short bacillus pumilus TW and Trichoderma according to claim 1 is characterized in that: said dispersant is the combination of any one or two kinds among sodium lignin sulfonate, neopelex, MF, NNO, the CNF.
3. the composition that contains short bacillus pumilus TW and Trichoderma according to claim 1 is characterized in that: said wetting agent is that sodium butylnaphthalenesulfonate, succinic acid Arrcostab sodium sulfonate, JFC, tea are withered, the combination of any one or two kinds in the spaonin powder, excrementum bombycis meal, washing powder.
4. the composition that contains short bacillus pumilus TW and Trichoderma according to claim 1 is characterized in that: said filler is the combination of any one or two kinds in kaolin, talcum powder, calcium carbonate, potter's clay, diatomite, bentonite, white carbon, pyrophyllite, the attapulgite.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102194154A CN102342300A (en) | 2011-08-02 | 2011-08-02 | Composition comprising brevibacillus brevis TW and trichodermin |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102194154A CN102342300A (en) | 2011-08-02 | 2011-08-02 | Composition comprising brevibacillus brevis TW and trichodermin |
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| CN102342300A true CN102342300A (en) | 2012-02-08 |
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| CN2011102194154A Pending CN102342300A (en) | 2011-08-02 | 2011-08-02 | Composition comprising brevibacillus brevis TW and trichodermin |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103563991A (en) * | 2012-07-31 | 2014-02-12 | 江苏丘陵地区镇江农业科学研究所 | Preparation method of brevibacillus brevis water dispersible granules |
| CN105567600A (en) * | 2015-10-21 | 2016-05-11 | 河南大学 | Pathogen verticillium antagonistic bacterium and application thereof |
| CN110325042A (en) * | 2016-11-03 | 2019-10-11 | 马罗内生物创新公司 | Except algae organism |
| CN112920954A (en) * | 2020-12-22 | 2021-06-08 | 慕恩（广州）生物科技有限公司 | Microbial compositions and uses thereof |
Citations (4)
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|---|---|---|---|---|
| CN1748511A (en) * | 2005-08-31 | 2006-03-22 | 山东靠山生物科技有限公司 | Biologica land biochemical preparation and its preparing method and use |
| CN101063094A (en) * | 2007-04-19 | 2007-10-31 | 江苏丘陵地区镇江农业科学研究所 | Short small bacillus TW and viable bacteria preparation for rice blast prevention and cure |
| CN101926366A (en) * | 2010-09-09 | 2010-12-29 | 北京科威拜沃生物技术有限公司 | Trichoderma and bacillus subtilis composite wettable powder |
| CN102125059A (en) * | 2010-12-03 | 2011-07-20 | 江苏苏滨生物农化有限公司 | Brevibacillus brevis TW effervescent granule and preparation method thereof |
-
2011
- 2011-08-02 CN CN2011102194154A patent/CN102342300A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1748511A (en) * | 2005-08-31 | 2006-03-22 | 山东靠山生物科技有限公司 | Biologica land biochemical preparation and its preparing method and use |
| CN101063094A (en) * | 2007-04-19 | 2007-10-31 | 江苏丘陵地区镇江农业科学研究所 | Short small bacillus TW and viable bacteria preparation for rice blast prevention and cure |
| CN101926366A (en) * | 2010-09-09 | 2010-12-29 | 北京科威拜沃生物技术有限公司 | Trichoderma and bacillus subtilis composite wettable powder |
| CN102125059A (en) * | 2010-12-03 | 2011-07-20 | 江苏苏滨生物农化有限公司 | Brevibacillus brevis TW effervescent granule and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 朱桂梅等: "短短小芽孢杆菌TW-2菌株对水稻穗颈瘟的防治效果", 《安徽农业科学》, vol. 35, no. 31, 31 December 2007 (2007-12-31), pages 9822 - 9823 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103563991A (en) * | 2012-07-31 | 2014-02-12 | 江苏丘陵地区镇江农业科学研究所 | Preparation method of brevibacillus brevis water dispersible granules |
| CN105567600A (en) * | 2015-10-21 | 2016-05-11 | 河南大学 | Pathogen verticillium antagonistic bacterium and application thereof |
| CN110325042A (en) * | 2016-11-03 | 2019-10-11 | 马罗内生物创新公司 | Except algae organism |
| CN112920954A (en) * | 2020-12-22 | 2021-06-08 | 慕恩（广州）生物科技有限公司 | Microbial compositions and uses thereof |
| CN112920954B (en) * | 2020-12-22 | 2023-02-10 | 慕恩（广州）生物科技有限公司 | Microbial compositions and uses thereof |
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Application publication date: 20120208 |