CN103563991A - Preparation method of brevibacillus brevis water dispersible granules - Google Patents
Preparation method of brevibacillus brevis water dispersible granules Download PDFInfo
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- CN103563991A CN103563991A CN201210271396.4A CN201210271396A CN103563991A CN 103563991 A CN103563991 A CN 103563991A CN 201210271396 A CN201210271396 A CN 201210271396A CN 103563991 A CN103563991 A CN 103563991A
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- brevibacillus brevis
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- water dispersible
- bactericide
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Abstract
The invention relates to a formulation of water dispersible granules of a rice blast biocontrol bacteria brevibacillus brevis bactericide and a preparation method thereof. The solid water dispersible granules contain brevibacillus brevis TW strain raw powder (2*10<12> cfu/g) and auxiliaries, wherein the auxiliaries comprise a carrier, a dispersant, a wetting agent, a disintegrating agent, a light protecting agent and a binding agent; a processing method comprises the following steps: mixing the active ingredient and probes of the auxiliaries in a certain proportion, crushing with an air flow, granulating and drying. The obtained brevibacillus brevis water dispersible granules of 4*10<10> cfu/g have a better control effect against rice blast.
Description
Technical field
The present invention relates to the preparation method of rice blast biocontrol microorganisms Brevibacillus brevis bactericide, especially relate to the preparation method of low consumption, high-content water-dispersion granula formulation.
Background technology
Rice blast claims again rice blast, burns pest, the pest of kowtowing, and is distributed in national Ge Dao district, main harm blade, stem stalk, fringe portion.Because Harm period, position difference are divided into seedling pest, leaf pest, joint pest, panicle blast, grain pest, very large on paddy growth impact, rice growth is fallen ill in earlier stage, can cause the withered of rice seedling, and heading stage, morbidity, can cause withered dead ears, had a strong impact on rice yield.To the effectively preventing method of rice blast, be chemical control, main medicament has tricyclazole, Isoprothiolane, carbendazim, thiophanate-methyl, rice blast peptide, Hinosan, iprobenfos, octicin solution, kasugarnycin etc.
Along with rice blast occurs more and more seriously, use chemical agent and be effectively preventing means the most in recent years, but use continuously chemical bactericide high dose, that the mode of action is single for a long time, easily bring that medicament is residual, the problem such as environmental pollution and resistance to pesticide resistance.The biological control of rice blast more and more comes into one's own, and many research units successively filter out effective biocontrol microorganisms.
Yang Jinghui ,Pan Yi building etc. discloses a kind of for preventing and treating the preparation of Brevibacillus brevis (Bacillus pumilus) the TW bacterial strain of rice blast in patent ZL2007 10021509.1, said preparation to the control efficiency of rice blast at 60%～80%.But study, show, the preparation of Brevibacillus brevis TW bacterial strain is larger on the control efficiency impact of disease on biocontrol microorganisms.And select suitable formulation, and one otherwise affect the survival of Brevibacillus brevis, two will be conducive to Brevibacillus brevis performance prophylaxis effect, and this top research contents is the present invention basis.
With chemical pesticide, compare, plant disease biocontrol bacteria exploitation kind is few, researches and develops evening time, and the research of existing bacteria preparation is all that technology and the method for using for reference chemical bactericide are carried out.Yet the character of biocontrol bacteria and sterilization mechanism and chemical bactericide have a great difference, imitate some processing technologys of chemical bactericide, the performance for biocontrol bacteria bacteriostasis, some is unnecessary or even harmful.
Summary of the invention
The invention provides a kind of overcome suspending agent and wetting powder shortcoming, good dispersion, easily preservation, convenient transportation, the preparation method of the dosage form of water dispersible granule rice blast Biological agents that the Brevibacillus brevis of low consumption, high-load is main active.
Technical scheme of the present invention:
The Brevibacillus brevis bactericide of dosage form of water dispersible granule provided by the invention, is characterized in that its principal component is the former powder of Brevibacillus brevis and auxiliary agent, and in the former powder of described Brevibacillus brevis, the former powder content of Brevibacillus brevis is 2 * 10
12cfu/g, described auxiliary agent comprises carrier, dispersant, wetting agent, disintegrant, bright protective agent, binding agent.
Described each ingredients weight parts proportioning is as follows:
1～50 part, the former powder of Brevibacillus brevis;
1～70 part, carrier;
1～20 part of dispersant;
0～20 part of wetting agent;
1～20 part of disintegrant;
0.5～20 part of bright protective agent;
1～20 part of binding agent.
The former powder of described Brevibacillus brevis is Brevibacillus brevis TW bacterial strain after fermentation, and after zymotic fluid adsorbs with precipitated calcium carbonate and dries after centrifugal, gemma content is 2 * 10
12the former powder of cfu/g.
Described carrier is one or more mixtures of White Carbon black, bentonite, kaolin, diatomite or calcium carbonate.
Described dispersant is a kind of surface reactive material, can be anion surfactant, cationic surfactant, amphoteric surfactant and non-ionic surface active agent.More preferably dodecyl sodium laurate, neopelex, lauryl sodium sulfate, lignin sodium and lignin calcium.
Described wetting agent is a kind of surface reactive material, can be anion surfactant, cationic surfactant, amphoteric surfactant and non-ionic surface active agent.More preferably dodecyl sodium laurate, neopelex, lauryl sodium sulfate, lignin sodium, lignin calcium and Tea Saponin.
Described disintegrant is Ac-Di-Sol, crosslinked carboxymethyl fecula sodium, ammonium sulfate, sodium bicarbonate, starch or urea.
Described bright protective agent fluorescent bleaches, india ink, nano titanium oxide, nano zine oxide or activated carbon powder.
Described binding agent is soluble starch, gelatin, polyvinyl alcohol, carboxymethyl cellulose or sodium carboxymethylcellulose.
The preparation method of dosage form of water dispersible granule Brevibacillus brevis bactericide provided by the invention, its step is as follows:
1) the former powder of Brevibacillus brevis, carrier, dispersant, wetting agent, disintegrant, bright protective agent and binding agent are mixed by above-mentioned weight portion proportioning, and through precomminution.
2) by the raw material through precomminution again through air-flow crushing, make its particle diameter be less than 20 μ m.
3) in mixer, the raw material through air-flow crushing is evenly mixed again.
4) add water and mediate, use comminutor extruder grain, or use thermopnore granulation.
5) afterwards it is carried out to vacuum drying or freeze drying, baking temperature is no more than 40 ℃, obtains moisture and is no more than 5% water dispersible granular thing.
6) the water dispersible granular thing obtaining is sieved, obtain Brevibacillus brevis water dispersible granules.
Brevibacillus brevis water dispersible granules of the present invention can be used separately, also can mix use with chemical bactericide, for preventing and treating rice blast.
Benefit of the present invention:
With respect to other formulation of biocontrol microorganisms, dosage form of water dispersible granule provided by the invention has the irreplaceable advantage of license: (1) process and farm work process dust pollution are very little, to operator's safety; (2) moisture little (being less than 5%), the biocontrol microorganisms biologically active retention time is long, and shelf life is long, constant product quality; (3) process environmental temperature is no more than 40 ℃, limit kept the activity of biocontrol microorganisms; (4) viable bacteria content high (1～2 * 1010cfu/g), protection effect is high; (5) adjuvant used all Water-borne modification material, adds that field consumption is very little, environmentally friendly, is the biological and ecological methods to prevent plant disease, pests, and erosion product of environmental protection.
Embodiment
Embodiment 1:
(gemma content is 2 * 10 to the former powder of Brevibacillus brevis TW bacterial strain
12cfu/g) 20g, lignin sulfonic acid sodium salt 10g, lignin calcium 10g, urea 5g, fluorescent whitening agent 2g, precipitated calcium carbonate 48g, carboxymethyl cellulose 5g.In cracker, be pulverized and mixed and become female powder, with airslide disintegrating mill, female powder is further pulverized, make particle diameter be less than 20 μ m, then in mixer, fully mix, add a certain amount of water, with extruder granulation, vacuum drying, baking temperature is controlled at 30 ℃.After detection moisture is less than 5%, sieve.Yield rate reaches 95%.Obtain 4 * 10
10cfu/g Brevibacillus brevis water dispersible granules.
Field is used, and gets 4 * 10
10cfu/g Brevibacillus brevis water dispersible granules 10g, is watered 40kg, spraying.After dispenser 20 days, the control efficiency of rice blast is reached to 79.5%.
Although above with general wash bright, specific embodiments and and effect experiment the present invention has been done to detailed description, on basis of the present invention, can to some modifications of do and improvement, this will be apparent to those skilled in the art.Therefore make without departing from theon the basis of the spirit of the present invention these modifications and improvement, all belong to the scope of protection of present invention.
Claims (4)
1. a dosage form of water dispersible granule Brevibacillus brevis bactericide, is characterized in that: comprise the former powder of Brevibacillus brevis and auxiliary agent.The Brevibacillus brevis gemma content of the former powder of described Brevibacillus brevis is 2 * 10
12cfu/g; Described auxiliary agent comprises carrier, dispersant, wetting agent, disintegrant, bright protective agent and binding agent; The weight proportion of described each component is as follows:
1～50 part, the former powder of Brevibacillus brevis;
1～70 part, carrier;
1～20 part of dispersant;
0～20 part of wetting agent;
1～20 part of disintegrant;
0.5～20 part of bright protective agent;
1～20 part of binding agent.
2. the bactericide of dosage form of water dispersible granule as claimed in claim 1, is characterized in that, active ingredient is the former powder of Brevibacillus brevis.
3. the Brevibacillus brevis bactericide of dosage form of water dispersible granule as claimed in claim 1, is characterized in that:
Described dispersant is anion surfactant, cationic surfactant, amphoteric surfactant and non-ionic surface active agent;
Described wetting agent is anion surfactant, cationic surfactant, amphoteric surfactant and non-ionic surface active agent;
Described disintegrant is Ac-Di-Sol, crosslinked carboxymethyl fecula sodium, ammonium sulfate, sodium bicarbonate, starch or urea;
Described bright protective agent is fluorescent bleaches, india ink, nano titanium oxide, nano zine oxide or activated carbon powder;
Described binding agent is soluble starch, gelatin, polyvinyl alcohol, carboxymethyl cellulose or sodium carboxymethylcellulose;
Described carrier is White Carbon black, bentonite, kaolin, diatomite or calcium carbonate.
4. the Brevibacillus brevis bactericide of dosage form of water dispersible granule as claimed in claim 1, is characterized in that: described dispersant is dodecyl sodium laurate, neopelex, lauryl sodium sulfate, lignin sodium, lignin calcium and Tea Saponin.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210271396.4A CN103563991A (en) | 2012-07-31 | 2012-07-31 | Preparation method of brevibacillus brevis water dispersible granules |
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| CN201210271396.4A CN103563991A (en) | 2012-07-31 | 2012-07-31 | Preparation method of brevibacillus brevis water dispersible granules |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103814892A (en) * | 2014-03-11 | 2014-05-28 | 江苏省苏科农化有限责任公司 | Bacillus amyloliquefaciens water dispersible granule |
| CN104396950A (en) * | 2014-11-17 | 2015-03-11 | 陕西恒田化工有限公司 | Water dispersible granule of bacillus methylotrophicus, preparation method and application thereof |
| CN105104048A (en) * | 2015-09-28 | 2015-12-02 | 江苏丘陵地区镇江农业科学研究所 | Method for inducing, identifying and screening rice blast resistance breeding materials |
| CN108795827A (en) * | 2018-07-06 | 2018-11-13 | 四川农业大学 | A kind of bacillus megaterium BM22 and its water dispersible granules and application |
| CN110396490A (en) * | 2019-08-15 | 2019-11-01 | 江苏苏滨生物农化有限公司 | A method of culture Brevibacillus brevis |
| CN112400870A (en) * | 2020-11-27 | 2021-02-26 | 云南省微生物发酵工程研究中心有限公司 | Bacillus licheniformis water dispersible granule and application thereof |
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| CN101278680A (en) * | 2007-04-06 | 2008-10-08 | 中国科学院动物研究所 | Insect baculovirus pesticides in dosage form of water dispersible granule and preparation |
| CN100513554C (en) * | 2007-04-19 | 2009-07-15 | 江苏丘陵地区镇江农业科学研究所 | Short small bacillus TW and viable bacteria preparation for rice blast prevention and cure |
| CN102038001A (en) * | 2010-12-03 | 2011-05-04 | 成都绿金高新技术股份有限公司 | Azadirachtin and bacillus subtilis mixed water dispersible granule |
| CN102342300A (en) * | 2011-08-02 | 2012-02-08 | 江苏丘陵地区镇江农业科学研究所 | Composition comprising brevibacillus brevis TW and trichodermin |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101278680A (en) * | 2007-04-06 | 2008-10-08 | 中国科学院动物研究所 | Insect baculovirus pesticides in dosage form of water dispersible granule and preparation |
| CN100513554C (en) * | 2007-04-19 | 2009-07-15 | 江苏丘陵地区镇江农业科学研究所 | Short small bacillus TW and viable bacteria preparation for rice blast prevention and cure |
| CN102038001A (en) * | 2010-12-03 | 2011-05-04 | 成都绿金高新技术股份有限公司 | Azadirachtin and bacillus subtilis mixed water dispersible granule |
| CN102342300A (en) * | 2011-08-02 | 2012-02-08 | 江苏丘陵地区镇江农业科学研究所 | Composition comprising brevibacillus brevis TW and trichodermin |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103814892A (en) * | 2014-03-11 | 2014-05-28 | 江苏省苏科农化有限责任公司 | Bacillus amyloliquefaciens water dispersible granule |
| CN103814892B (en) * | 2014-03-11 | 2015-05-27 | 江苏省苏科农化有限责任公司 | Bacillus amyloliquefaciens water dispersible granule |
| CN104396950A (en) * | 2014-11-17 | 2015-03-11 | 陕西恒田化工有限公司 | Water dispersible granule of bacillus methylotrophicus, preparation method and application thereof |
| CN104396950B (en) * | 2014-11-17 | 2016-03-02 | 陕西恒田化工有限公司 | Water dispersible granules of a kind of Methylotrophic bacillus and its preparation method and application |
| CN105104048A (en) * | 2015-09-28 | 2015-12-02 | 江苏丘陵地区镇江农业科学研究所 | Method for inducing, identifying and screening rice blast resistance breeding materials |
| CN108795827A (en) * | 2018-07-06 | 2018-11-13 | 四川农业大学 | A kind of bacillus megaterium BM22 and its water dispersible granules and application |
| CN110396490A (en) * | 2019-08-15 | 2019-11-01 | 江苏苏滨生物农化有限公司 | A method of culture Brevibacillus brevis |
| CN110396490B (en) * | 2019-08-15 | 2020-10-09 | 江苏苏滨生物农化有限公司 | Method for culturing brevibacillus brevis |
| CN112400870A (en) * | 2020-11-27 | 2021-02-26 | 云南省微生物发酵工程研究中心有限公司 | Bacillus licheniformis water dispersible granule and application thereof |
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Application publication date: 20140212 |