CN104396950B - Water dispersible granules of a kind of Methylotrophic bacillus and its preparation method and application - Google Patents
Water dispersible granules of a kind of Methylotrophic bacillus and its preparation method and application Download PDFInfo
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Abstract
The present invention relates to a kind of water dispersible granules effectively can preventing and treating the Methylotrophic bacillus of crop disease viral disease, and the preparation method and application of described water dispersible granules.Described water dispersible granules comprises following component: the female medicine 1-40% of Methylotrophic bacillus, dispersant 1-10%, wetting agent 0.5-5%, disintegrant 1-15%, binding agent 1-4%, carrier add to 100%; Described wetting agent is SUPRAGLLWP, does is described dispersant SUPRAGLL? MNS/90, described % is weight percentage.SUPRAGLL is adopted in the present invention's formula? MNS/90 as dispersant, adopt SUPRAGLLWP as wetting agent, owing to adopting described specific dispersant and wetting agent, obtained water dispersible granules quality performance indicators checks its dispersiveness, wetability, suspensibility, frictional dissipation index all to have larger improvement.
Description
Technical field
The present invention relates to pesticide field, particularly bactericide field, relate to a kind of water dispersible granules effectively can preventing and treating the Methylotrophic bacillus of crop disease viral disease particularly, and the preparation method and application of described water dispersible granules.
Technical background
Methylotrophic bacillus (Bacillusmethylotrophicus) is a kind in bacillus.Gram-positive, glucose, arabinose, catalase, lipase, urase and oxidase are all positive, aerobic, shaft-like, possess locomitivity.
Research finds there is not common toxin gene (HBL, BcET, NHE, entFM), sphingomyelinase (Sph), phosphatidase (PI-PLC) in Bacillus strain; Also not with NHE, HBL antibody response.Think that it is guaranteed for being separated from traditional fermented food Bacillus strain in safety.
In China's agricultural fast development process, sick worm harm is the key factor of restriction China Sustainable Agricultural stable development.In recent years, China's corps diseases annual occurring area is more than 100,000,000 hectares, and the sick worm damage losses of annual grain is about more than 3,000 ten thousand tons, causes China's economic loss up to more than 300 hundred million.According to statistics, China is reached 9,330,000 more than hm2 by the farmland of pesticide pollution, and that die from pesticide pollution food every year is 0.83 ten thousand people, and the direct economic loss caused because of pesticide pollution is every year just up to more than 160 hundred million yuans.Utilize public nuisance-free agricultural chemicals controlling plant diseases, reduce chemical pesticide usage amount and alleviate pesticide pollution, ensure that food security and Development of Organic Agriculture have become China's developing modern agriculture and the organic farming task of top priority in this century.
On the other hand, in recent years, along with the change of crop varieties, planting conditions, more original bacterial disease of too many attention that do not cause becomes important disease gradually.Such as, the harm of the bacterial disease such as tobacco bacterial wilt, bacterial blight of rice, soft rot of Chinese cabbage is more and more serious.
Bacillus is because of its safety and can produce multiple antibacterial material and enzyme viability and be subject to the preference of biological control worker.And can bacteria agent be processed into through cultivation and fermentation, Application and Development potentiality are large.More existing excellent Bacillus strains are applied to production practices at present.
Methylotrophic bacillus is the one of bacillus, and having height, to kill bacterial activity, fungicidal spectrum wide and have the environmentally friendly biological bacterial strain of growth-promoting functions.
Summary of the invention
The present invention solves the deficiency in above-mentioned technical background, provides water dispersible granules of the bacterial diseases such as a kind of effective control tobacco bacterial wilt, bacterial blight of rice, soft rot of Chinese cabbage and preparation method thereof.
For achieving the above object, the present invention is achieved by the following technical solutions.
The water dispersible granules of a kind of Methylotrophic bacillus, it is characterized in that, described water dispersible granules comprises following component: the female medicine 1-40% of Methylotrophic bacillus, dispersant 1-10%, wetting agent 0.5-5%, disintegrant 1-15%, binding agent 1-4%, carrier add to 100%; Described wetting agent is SUPRAGLLWP, and described dispersant is SUPRAGLLMNS/90, and described % is weight percentage.
The preparation of the female medicine of described Methylotrophic bacillus and extracting method be according to Chinese patent 201210579899, denomination of invention be in " bacteria agent of a kind of Methylotrophic bacillus and its preparation method and application " described in method obtain.Described wetting agent SUPRAGLLWP and polycarboxylic acid salt's dispersant SUPRAGLLMNS/90 all can purchased from Rhodia company.
Described disintegrant is ammonium sulfate, sodium sulphate, one or more in sodium carbonate, sodium bicarbonate, ammonium carbonate, ammonium chloride, diammonium hydrogen phosphate;
Described binding agent is one or more in starch, polyethylene glycol, pyrrolidones, polyvinyl alcohol, urea;
Described carrier is kaolin, atlapulgite, diatomite, mud coal, precipitated calcium carbonate, White Carbon black, pyrophillite, talcum powder, one or more in calcite.
Preferably, the consumption of described wetting agent is 1-3%, is more preferably 2.5%;
Preferably, the consumption of described dispersant is 1-8%, is more preferably 6.0%;
Further, the invention still further relates to the preparation method of described Methylotrophic bacillus water dispersible granules, it is characterized in that, described preparation method comprises and will drop in mixer by female for Methylotrophic bacillus medicine, dispersant, wetting agent, disintegrant and carrier according to consumption, mix rear pulverizing, the aqueous solution (aqueous solution of 2%) of the material after pulverizing and binding agent is mediated; Material after kneading is through granulator granulation; Remove the particle being greater than 5mm and being less than 0.5mm, dry.The granulation again after again mediating of inappropriate particle.
Further, the invention still further relates to the application that described Methylotrophic bacillus water dispersible granules is used as bactericide.
Compared with prior art, the advantage that has of the present invention and effect as follows:
1. the pesticide water dispersible granule of the present invention, no dust pollution, environmental friendliness, height kills vegetative bacteria activity, nontoxic advantage.
2. adopt SUPRAGLLMNS/90 as dispersant in the present invention's formula, adopt SUPRAGLLWP as wetting agent, owing to adopting described specific dispersant and wetting agent, obtained water dispersible granules quality performance indicators checks its dispersiveness, wetability, suspensibility, frictional dissipation index all to have larger improvement.
Embodiment
For make technical scheme of the present invention be convenient to understand, below in conjunction with concrete test example to Methylotrophic bacillus water dispersible granules preparation and should be used as to illustrate.
Embodiment 1. prepares 10% Methylotrophic bacillus water dispersible granules germ killing drugs (1000 kilograms)
Prepared by Methylotrophic bacillus:
The wetting agent SUPRAGLLWP that embodiment adopts and polycarboxylic acid salt's dispersant SUPRAGLLMNS/90 is all purchased from Rhodia company;
Methylotrophic bacillus is that method described in " bacteria agent of a kind of Methylotrophic bacillus and its preparation method and application " obtains according to Chinese patent 201210579899, denomination of invention.
1-1 conventional formulation (contrast 1): principal component Methylotrophic bacillus is 1%, namely 10 kilograms.And containing following various auxiliary agent, dispersant T36 is 5%, namely 50 kilograms, dispersant 27003%, namely 30 kilograms; Wetting agent lauryl sodium sulfate 3%, namely 30 kilograms; Disintegrant diammonium hydrogen phosphate 3%, namely 30 kilograms; Binding agent polyethylene glycol 3%, namely 30 kilograms; Carrier white carbon is 140 kilograms, and carrier kaolin surplus is 680 kilograms.
1-2 newly fills a prescription (formula of the present invention): principal component Methylotrophic bacillus is 1%, namely 10 kilograms.And containing following various auxiliary agent, dispersant SUPRAGLLMNS/90 is 8%, namely 80 kilograms; Wetting agent SUPRAGLLWP is 3.0%, namely 30 kilograms; Disintegrant diammonium hydrogen phosphate 3%, namely 30 kilograms; Binding agent polyethylene glycol 3%, namely 30 kilograms; Carrier white carbon is 140 kilograms, and carrier kaolin surplus is 680 kilograms.
1-3 control formula (contrast 2): except wetting agent is lauryl sodium sulfate, all the other fill a prescription identical with the invention described above;
1-4 control formula (contrast 3): except dispersant is T36, all the other fill a prescription identical with the invention described above;
Preparing process is: measured material of falling into a trap filling a prescription above drops in mixer, stirs, pulverizes through airslide disintegrating mill.Material after pulverizing and the aqueous solution of binding agent polyethylene glycol are mediated, is kneaded into after paste through granulator granulation; 5mm and the particle removal being less than 0.5mm will be greater than with sieve; Suitable particle drying, detects qualified rear packaging.Inappropriate particle is granulation again after kneader is mediated again.
The water dispersible granules quality performance indicators Interventions Requested that above-mentioned 4 formulas are obtained and index see attached list
1from table
1known, its dispersiveness of product, wetting time, suspending agent and the frictional dissipation performance indications that adopt new dispersant SUPRAGLLMNS/90 and wetting agent SUPRAGLLWP to obtain all are better than the product obtained by conven-tional adjuvants.Simultaneously as can be seen from 1-3 control formula as a comparison or 1-4 control formula, adopt above-mentioned wetting agent or the water dispersible granules prepared by dispersant separately, its various aspects of performance is also far below effect of the present invention.
Table
1the water dispersible granules quality performance indicators inspection contrast that the different auxiliary formula of Methylotrophic bacillus is obtained
| Quality index | Formula 1-1 | Formula 1-2 | Formula 1-3 | Formula 1-4 | Remarks |
| Dispersiveness/% >= | 80 | 90 | 80 | 82 | Vaporific smear effects |
| Wetting time/s≤ | 60 | 30 | 55 | 62 | CIPACMT185 |
| Suspensibility/% >= | 90 | 96 | 92 | 91 | CIPACMT184 |
| Frictional dissipation/g≤ | 1.0 | 0.2 | 0.9 | 1.0 | ASTM standard screen |
Embodiment 2. prepares 40% Methylotrophic bacillus water dispersible granules germ killing drugs (1000 kilograms)
2-1 conventional formulation (contrast 1): principal component Methylotrophic bacillus is 40%, namely 400 kilograms.And containing following various auxiliary agent, dispersant T50 is 8%, namely 80 kilograms; Wetting agent lauryl sodium sulfate 3%, namely 30 kilograms; Disintegrant diammonium hydrogen phosphate 3%, namely 30 kilograms; Binding agent polyethylene glycol 3%, namely 30 kilograms; Carrier white carbon is 140 kilograms, and carrier kaolin surplus is 290 kilograms.
2-2 newly fills a prescription (formula of the present invention): principal component Methylotrophic bacillus is 40%, namely 400 kilograms.And containing following various auxiliary agent, dispersant SUPRAGLLMNS/90 is 10%, namely 100 kilograms; Wetting agent SUPRAGLLWP is 0.5%, namely 5 kilograms; Disintegrant ammonium chloride 10%, namely 100 kilograms; Binding agent urea 4%, namely 40 kilograms; Carrier talc surplus is 355 kilograms.
2-3 control formula (contrast 2): except wetting agent is lauryl sodium sulfate, all the other fill a prescription identical with the invention described above;
2-4 control formula (contrast 3): except dispersant is T50, all the other fill a prescription identical with the invention described above;
Preparing process is with embodiment 1
The obtained water dispersible granules quality performance indicators Interventions Requested of this formula and index is adopted to see attached list
2from table
2known, the product adopting dispersant SUPRAGLLMNS/90 and SUPRAGLLWP to obtain its dispersiveness, wetting time and suspending agent performance indications are all obviously better than the product obtained by conven-tional adjuvants.
Table
2the water dispersible granules quality performance indicators inspection contrast that the different auxiliary formula of Methylotrophic bacillus is obtained
| Quality index | Formula 2-1 | Formula 2-2 | Formula 2-3 | Formula 2-4 | Remarks |
| Dispersiveness/% >= | 85 | 95 | 70 | 85 | Vaporific smear effects |
| Wetting time/s≤ | 60 | 40 | 50 | 50 | GB/T5451 |
| Suspensibility/% >= | 80 | 98 | 85 | 85 | GB/T14825-2006 |
| Frictional dissipation/g≤ | 1.5 | 0.1 | 0.5 | 1.0 | ASTM standard screen |
Embodiment 3. prepares 12% Methylotrophic bacillus water dispersible granules germ killing drugs (1000 kilograms)
3-1 conventional formulation (contrast 1): principal component Methylotrophic bacillus is 12%, i.e. 120 kg.And containing following various auxiliary agent, dispersant T318%, namely 80 kilogram; Wetting agent lauryl sodium sulfate 5%, namely 50 kilograms; Disintegrant sodium sulphate 15%, namely 150 kilograms; Binding agent pyrrolidones 1%, namely 10 kilograms; Carrier white carbon is 5%, namely 50 kilograms, and carrier precipitated calcium carbonate surplus is 540 kilograms.
3-2 newly fills a prescription (formula of the present invention): principal component Methylotrophic bacillus is 12%, i.e. 120 kg.And containing following various auxiliary agent, dispersant SUPRAGLLMNS/90 is 10%, namely 100 kilograms; Wetting agent SUPRAGLLWP is 0.5%, namely 5 kilograms; Disintegrant ammonium chloride 10%, namely 100 kilograms; Binding agent urea 4%, namely 40 kilograms; Carrier talc surplus is 635 kilograms.
3-3 control formula (contrast 2): except wetting agent is lauryl sodium sulfate, all the other fill a prescription identical with the invention described above;
3-4 control formula (contrast 3): except dispersant is T31, all the other fill a prescription identical with the invention described above;
Preparing process is with embodiment 1
The obtained water dispersible granules quality performance indicators Interventions Requested of this formula and index is adopted to see attached list
3from table
3known, the product adopting dispersant SUPRAGLLMNS/90 and wetting agent SUPRAGLLWP to obtain its dispersiveness, wetting time and suspending agent performance indications are all obviously better than the product obtained by conven-tional adjuvants.
The water dispersible granules quality performance indicators inspection contrast that the different auxiliary formula of table 3 Methylotrophic bacillus is obtained
| Quality index | Formula 3-1 | Formula 3-2 | Formula 3-3 | Formula 3-4 | Remarks |
| Dispersiveness/% >= | 85 | 95 | 80 | 80 | Vaporific smear effects |
| Wetting time/s≤ | 50 | 30 | 50 | 55 | GB/T5451 |
| Suspensibility/% >= | 85 | 98 | 85 | 85 | GB/T14825-2006 |
| Frictional dissipation/g≤ | 1.3 | 0.1 | 0.6 | 1.0 | ASTM standard screen |
Embodiment 4. prepares 30% Methylotrophic bacillus water dispersible granules germ killing drugs (1000 kilograms)
4-1 conventional formulation (contrast 1): principal component Methylotrophic bacillus is 30%, namely 300 kilograms.And containing following various auxiliary agent, dispersant 27002%, namely 20 kilogram, dispersant NNO6%, namely 60 kilograms; Wetting agent LWET/F2%, namely 20 kilograms; Disintegrant amine sulfate 9%, namely 90 kilograms; Adhesive starch 3%, namely 30 kilograms; Carrier diatomaceous earth surplus is 480 kilograms.
4-2 newly fills a prescription (formula of the present invention): principal component Methylotrophic bacillus is 30%, namely 300 kilograms.And containing following various auxiliary agent, dispersant SUPRAGLLMNS/90 is 10%, namely 100 kilograms; Wetting agent SUPRAGLLWP is 0.5%, namely 5 kilograms; Disintegrant ammonium chloride 10%, namely 100 kilograms; Binding agent urea 4%, namely 40 kilograms; Carrier talc surplus is 455 kilograms.
4-3 control formula (contrast 2): except wetting agent is LWET/F, all the other fill a prescription identical with the invention described above;
4-4 control formula (contrast 3): except dispersant is except 2700, all the other fill a prescription identical with the invention described above;
Preparing process is with embodiment 1
The obtained water dispersible granules quality performance indicators Interventions Requested of this formula and index is adopted to see attached list
4from table
4known, the product adopting SUPRAGLLMNS/90 and wetting agent SUPRAGLLWP to obtain its dispersiveness, wetting time and suspending agent performance indications are all obviously better than the product obtained by conven-tional adjuvants.
Table
4the water dispersible granules quality performance indicators inspection contrast that the different auxiliary formula of Methylotrophic bacillus is obtained
| Quality index | Formula 4-1 | Formula 4-2 | Formula 4-3 | Formula 4-4 | Remarks |
| Dispersiveness/% >= | 85 | 90 | 80 | 85 | Vaporific smear effects |
| Wetting time/s≤ | 60 | 30 | 55 | 55 | GB/T5451 |
| Suspensibility/% >= | 85 | 98 | 85 | 90 | GB/T14825-2006 |
| Frictional dissipation/g≤ | 2.0 | 0.5 | 1.0 | 1.5 | ASTM standard screen |
Methylotrophic bacillus water dispersible granules is to the activity research of phytobacterial disease:
1. indoor biometrics test:
Good preventive and therapeutic effect is had to tobacco bacterial wilt by indoor biometrics experimental study Methylotrophic bacillus water dispersible granules.
1.1 reagent agents: Methylotrophic bacillus water dispersible granules (Heng Tian Inc. is standby)
Contrast medicament: 72% agricultural streptomycin sulphate wetting powder.(commercial)
1.2 test soil:
Mixture Nutrition Soil V (soil): V (the Nutrition Soil)=1:l of sterilizing.
1.2.1 for examination pathogen: tobacco ralstonia solanacearum Ralstoniasolanacearum, TTC medium is cultivated, makes 1 × 106cfu/ml bacteria suspension, stand-by.
1.3 test plants: cultivate cigarette seedling to the 4 ~ 5 leaf phase at sterilized soil, take out inoculation tobacco Ralstonia solanacearum by seedling.Inoculum concentration is 5ml/ strain.
1.4 processing methods:
A. blank, uses clear water.
B. soak seed: by Methylotrophic bacillus water-dispersible grain dilution agent 200 times, prior to seeding seed is soaked 1 day.
C. fill with root: in the tobacco growing middle and later periods, by Methylotrophic bacillus water-dispersible grain dilution agent 200 times, medicament directly sprays at axis base portion by 72% agricultural streptomycin sulphate wetting powder, 2000 times of liquid.
Often 3 repetitions are established in process, often repeat 10 strain cigarette seedlings, and process is transplanted in the seedling alms bowl that sterilized soil is housed, under process is positioned over 28 ~ 36 DEG C, greenhouse condition, and 15 days " Invest, Then Investigate " tobacco plant incidences, the statistics incidence of disease and disease index.Calculate preventive effect.
1.5 result of the tests and analysis:
Tobacco ralstonia solanacearum seed dispersal disease effectively can be prevented and treated by method for soaking seed known Methylotrophic bacillus water dispersible granules.Average preventive effect is more than 93%.For details see attached table 1
Tobacco growing phase bacterial wilt evil effectively can be prevented and treated by root-pouring method known Methylotrophic bacillus water dispersible granules.Average preventive effect is more than 90%.For details see attached table 2
Table 1 Methylotrophic bacillus water dispersible granules seed soaking control tobacco bacterial wilt effect
Table 2 Methylotrophic bacillus water dispersible granules fills with root control tobacco bacterial wilt effect
2. Methylotrophic bacillus water dispersible granules field medicine effect test:
2.1 for studying thing:
Tobacco, paddy rice, Chinese cabbage.
Controlling object: tobacco bacterial wilt, bacterial blight of rice, soft rot of Chinese cabbage.
2.2 disease scales and computational methods:
Stage division:
0 grade: complete stool is anosis
1 grade: stem is even to be had chlorisis scab or have minority blade wilting in streak side
3 grades: stem's black scab.But do not reach top, or sick side 5% blade is wilting
5 grades: stem's black scab reaches plant top.Or sick side 2/3 blade is wilting
7 grades: diseased plant stem is withered
2.3 Methylotrophic bacillus water dispersible granules field medicine effect tests are implemented:
(1), the tobacco test of pesticide effectiveness:
Experimental field: be selected in Baoji, for examination ground area 1.36 mu, soil property is light loam, and the content of organic matter is 1.01%, pH value 7.7, all experimental plots cultivation condition and control measures consistent.
Chemicals treatment: totally 3 process of Methylotrophic bacillus water dispersible granules 200 times of liquid, 72% agricultural streptomycin sulphate wetting powder and not dispenser clear water are established in experiment, each process 4 repetition, Gong12Ge community, each community randomized arrangement, 40m2 minizone, each community and experimental field surrounding arrange the wide isolation strip of 0.6m.
For examination tobacco planting: on April 5th, 2013 is sowed, average spacing in the rows 8cm.
Application method: after planting fill with once respectively on May 5th, 2013,15 days and 25 days root, mu liquid 500kg, chemical sprayer tool is workers and peasants---16 type sprayers, take off sprinkling irrigation by shower nozzle.
Local meteorological condition:
First time dispenser (May 5) is fine, and wind-force 3 grades, temperature 15-25 DEG C, relative moisture is 80%.Second time dispenser (May 15) is cloudy, and wind-force 3 grades, temperature 15-23 DEG C, relative moisture is 80%.Third time dispenser (May 25) is cloudy, and wind-force 5 grades, maximum air temperature 14-25 DEG C, relative moisture is 80%.
Result of the test investigation method: investigate for 7 days after last dispenser, every community adopts 4 point samplings, looks into 20 strains at often, records total strain number and diseased plant number at different levels.
Result and analysis: as shown in table 3 below.
Table 3 Methylotrophic bacillus water dispersible granules prevents and treats situation to tobacco bacterial wilt field trial
Field control effectiveness test result shows, the average preventive effect of Methylotrophic bacillus water dispersible granules to tobacco bacterial wilt reaches more than 90%.Do not find in process of the test that reagent agent Methylotrophic bacillus water dispersible granules has any harmful effect to tobacco plants growth.
(2), the paddy rice test of pesticide effectiveness:
Experimental field:
Be selected in Yang County, Shaanxi, practice ground 800m2 (1.21 mu), soil property is light loam, and the content of organic matter is 1.2%, pH value 7.5, all experimental plots cultivation condition and control measures consistent;
Chemicals treatment: totally 3 process of Methylotrophic bacillus water dispersible granules 200 times of liquid, Prochloraz 500 times of liquid and not dispenser clear water are established in this experiment, each process 4 repetition, Gong12Ge community, each community randomized arrangement, 60m2 minizone, each community and experimental field surrounding arrange the wide isolation strip of 0.5m.
Rice Cropping situation: in March, 2010 is sowed, average spacing in the rows 7cm.
Application method: respectively on April 15th, 2010, May 5 and 25 days, root was filled with once respectively after insemination and emergence, mu liquid 500kg, chemical sprayer tool is workers and peasants---16 type sprayers, take off sprinkling irrigation by shower nozzle.
Local meteorological condition:
Dispenser (April 15) same day first time is partly cloudy, and wind-force 3 grades, the maximum air temperature 14 DEG C, the minimum air temperature 1 DEG C, relative moisture is 30%.Second time was cloudy for dispenser (May 15) same day, and wind-force 3 grades, the maximum air temperature 19 DEG C, the minimum air temperature 3 DEG C, relative moisture is 30%.Dispenser (May 25) same day third time is partly cloudy, and wind-force 5 grades, the maximum air temperature 23 DEG C, the minimum air temperature 10 DEG C, relative moisture is 20%.
Result of the test is investigated: within 7 days, investigate after last dispenser, every community adopts 4 point samplings, looks into 20 strains at often, records total strain number and diseased plant number at different levels.
Result and analysis:
As shown in table 4 below, wherein, all sample points all do not have poisoning to occur, and show through field plot trial, and the average preventive effect of Methylotrophic bacillus water dispersible granules to bacterial blight of rice reaches 92.3%, higher than the preventive effect of Prochloraz missible oil.
Table 4 Methylotrophic bacillus water dispersible granules prevents and treats situation to field trials of rice bacterial blight
(3), the Chinese cabbage test of pesticide effectiveness:
Experimental field:
Be selected in Yangling Shaanxi, practice ground 800m2 (1.21 mu), soil property is light loam, and the content of organic matter is 1.07%, pH value 7.2, all experimental plots cultivation condition and control measures consistent.
Chemicals treatment: totally 3 process of Methylotrophic bacillus water dispersible granules 200 times of liquid, agricultural streptomycin 4000 times of liquid and not dispenser clear water are established in this experiment, each process 4 repetition, Gong12Ge community, each community randomized arrangement, 40m2 minizone, each community and experimental field surrounding arrange the wide isolation strip of 0.6m.
Growth of Chinese cabbage situation: in July, 2010 is sowed, average spacing in the rows 8cm.
Application method: after planting fill with once respectively on August 5th, 2010,15 days and 25 days root, mu liquid 500kg, chemical sprayer tool is workers and peasants---16 type sprayers, take off sprinkling irrigation by shower nozzle.
Local meteorological condition:
Dispenser (August 5) same day first time is partly cloudy, and wind-force 3 grades, the maximum air temperature 25 DEG C, the minimum air temperature 15 DEG C, relative moisture is 80%.Second time was cloudy for dispenser (August 15) same day, and wind-force 3 grades, the maximum air temperature 23 DEG C, the minimum air temperature 15 DEG C, relative moisture is 80%.Dispenser (August 25) same day first time is partly cloudy, and wind-force 5 grades, the maximum air temperature 25 DEG C, the minimum air temperature 14 DEG C, relative moisture is 80%.
Result of the test is investigated: last dispenser was investigated after 10 days, and every community adopts 4 point samplings, looks into 20 strains at often, records total strain number and diseased plant number at different levels.
Investigation result is as shown in table 5 below, and result shows that the average preventive effect of Methylotrophic bacillus water dispersible granules to soft rot of Chinese cabbage reaches 89.6%, higher than agricultural streptomycin 4000 times of liquid.
The field trial of table 5 Methylotrophic bacillus water dispersible granules to soft rot of Chinese cabbage prevents and treats situation
Water dispersible granules of a kind of Methylotrophic bacillus of the present invention and its preparation method and application is described by concrete example, those skilled in the art can use for reference content of the present invention, the links such as suitable feed change, process conditions realize other object corresponding, its relevant change does not all depart from content of the present invention, all similar replacements and change will become apparent to those skilled in the art that and be all deemed to be included within scope of the present invention.
Claims (6)
1. the water dispersible granules of a Methylotrophic bacillus, it is characterized in that, described water dispersible granules comprises following component: the female medicine 1-40% of Methylotrophic bacillus, dispersant 6%, wetting agent 2.5%, disintegrant 1-15%, binding agent 1-4%, carrier add to 100%; Described wetting agent is SUPRAGILWP, and described dispersant is SUPRAGILMNS/90, and described % is weight percentage.
2. water dispersible granules according to claim 1, is characterized in that, described disintegrant is ammonium sulfate, sodium sulphate, one or more in sodium carbonate, sodium bicarbonate, ammonium carbonate, ammonium chloride, diammonium hydrogen phosphate.
3. water dispersible granules according to claim 1, is characterized in that, described binding agent is one or more in starch, polyethylene glycol, pyrrolidones, polyvinyl alcohol, urea.
4. water dispersible granules according to claim 1, is characterized in that, described carrier is kaolin, atlapulgite, diatomite, mud coal, precipitated calcium carbonate, White Carbon black, pyrophillite, talcum powder, one or more in calcite.
5. according to the preparation method of the arbitrary described water dispersible granules of Claims 1-4, it is characterized in that, described preparation method comprises and will drop in mixer by female for Methylotrophic bacillus medicine, dispersant, wetting agent, disintegrant and carrier according to consumption, mix rear pulverizing, the aqueous solution of the material after pulverizing and binding agent is mediated; Material after kneading is through granulator granulation; Remove the particle being greater than 5mm and being less than 0.5mm, dry.
6. the application of bactericide is used as according to the arbitrary described water dispersible granules of Claims 1-4.
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