CN110402966A - A kind of controlling disease tearing wax pore fungi solid fungicide, preparation method and applications - Google Patents
A kind of controlling disease tearing wax pore fungi solid fungicide, preparation method and applications Download PDFInfo
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- CN110402966A CN110402966A CN201910693661.XA CN201910693661A CN110402966A CN 110402966 A CN110402966 A CN 110402966A CN 201910693661 A CN201910693661 A CN 201910693661A CN 110402966 A CN110402966 A CN 110402966A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
Abstract
The present invention provides a kind of tearing wax pore fungi solid fungicide, the tearing wax pore fungi solid fungicide is made of viable bacteria and solid matrix, and the viable bacteria is tearing wax pore fungi HG2011, and the thallus viable count of viable bacteria is 3.8*108cfu/g‑7.1*109cfu/g.The present invention tears wax pore fungi HG2011 feature, and tearing wax pore fungi HG2011 solid fungicide has the function of that significant growth-promoting, volume increase and raising quality have opened up the new way for producing a kind of growth-promoting diseases prevention.
Description
Technical field
The invention belongs to field of agricultural microorganism, and in particular to a kind of to utilize citrus peel residue production tearing wax pore fungi solid bacterium
The method and its application of agent.
Background technique
The usage amount of China's fertilizer and pesticide occupies first of countries in the world, and the fertilizer and pesticide investment of a large amount brings a series of rings
Border, ecology and food-safety problem.Agriculture rural area portion in 2018 proposes to strive that the year two thousand twenty fertilizer and pesticide usage amount realizes negative increase
It is long, subtract the great research field that medicine is listed in national science and technology development.Biocontrol microorganisms mainly pass through secretion antibacterial material, with cause of disease
Bacterium competes Vegetation space, kills or inhibits pathogenic microorganism, or induction plant to obtain resistance, improve plant autoimmunity, reach
To Shaoshi or no pesticide grown；Promoting bacteria can secrete growth-promoting substance such as auxins, promote plant growth, and activating soil slightly solubility is supported
Point, such as Soluble phosphorus, potassium decomposing, nutrient availability is improved, the purpose subtracted to fertilize is reached.Therefore the efficient biocontrol microorganisms of screening, Promoting bacteria or simultaneous
Having the microbial strains of two kinds of functions and establishing corresponding matched production technology is the work urgently innovated and current agriculture
One of industry scientific research and the hot spot of application.
The research team of applicant independently separates the tearing wax pore fungi new strains HG2011 of acquisition, and it was found that lid strain can
Secrete beta-1,3 glucanase, chitinase, phosphatase, small molecule antibacterial substance, siderophore, auxin (IAA) and a variety of organic
Acid etc. has the functions such as disease-resistant, growth-promoting, Soluble phosphorus, potassium decomposing, has both biological and ecological methods to prevent plant disease, pests, and erosion and growth-promoting functions.
Applicant will be by tearing wax pore fungi HG2011 in Chinese microorganism strain preservation conservator on June 2nd, 2017
Meeting common micro-organisms center has carried out preservation (C.lacerate HG2011, deposit number are CGMCC No.13899).
Applicant has carried out application study for tearing wax pore fungi HG2011, and applicant submits China on 07 14th, 2017
Patent application " one plant of tearing wax hole bacteria strain and its effect (CN201710576686.2) for preventing and treating crop fungal disease ", China
" one plant tears the growth-promoting functions of wax hole bacteria strain and its applies (CN201710576704.7): disclosing one kind and tears for patent application
The liquid bacterial agent for splitting wax pore fungi discloses application effect of the tearing wax pore fungi HG2011 fermentation liquid on capsicum, flue-cured tobacco, eggplant.
But aforesaid liquid microbial inoculum is found in practical applications.Since fermentation liquid exists, storage is difficult, volume is big, is not easy to transport
And the defects of secondary fermentation, it is difficult to promote and apply.
Applicant research team in 2018 reports tearing wax pore fungi solid on the 12nd phase of " Scientia Agricultura Sinica " periodical
A formula (mass ratio that husk, corn flour, vermiculite and water press 15 ﹕, 3 ﹕, 15 ﹕ 70) for microbial inoculum and this solid fungicide are to eggplant
The control efficiency and production-increasing function of sub- cotton disease, vermiculite are that a kind of non-renewable resources are unable to the lasting use of section.In addition, vermiculite
Degradability as water absorbing agent is poor, is used for a long time to soil environment by reversible injury, discovery should be somebody's turn to do in practical applications
That there are stability is poor for the tearing wax pore fungi solid fungicide of the culture of matrix, to farmland by adverse effect the defects of.
Summary of the invention
Based on the reason of the background technique, while the production material for gathering solid fungicide can make full use of in agricultural production process
Organic waste, not only reduced environmental pollution, but also advantageous agricultural wastes recycle.Illustrate using in agricultural production process
Organic waste production tearing wax pore fungi solid fungicide feasibility.
In screening host material, applicant has screened more than 30 materials, and tearing wax pore fungi is white-rot fungi, lignin,
The high matrix of cellulose is its ideal carbon source, it is found by the applicant that citrus peel residue is faintly acid, it is suitable for tearing the growth of wax pore fungi；Together
When citrus peel residue include a large amount of celluloses, hemicellulose and pectin, exactly tearing wax pore fungi grow required for nutritional ingredient；Newly
Fresh citrus peel residue water content is 60-80%, does not need addition vermiculite and adjusts moisture content.Therefore, citrus peel residue is with proper amount of
Crop material collection provides nutrition, keeps moisture, and suitable growth pH environment is integrated；Therefore it is developed based on by citrus peel residue
The matrix of tearing wax pore fungi solid fungicide.
The present invention is with a kind of tearing wax pore fungi solid fungicide of citrus peel residue matrix core, the tearing wax pore fungi solid bacterium
Agent is made of viable bacteria and solid matrix, and the viable bacteria is tearing wax pore fungi HG2011, and the thallus viable count of viable bacteria is 3.8*
108cfu/g-7.1*109cfu/g。
The solid matrix main component is that citrus peel residue and crop material form, and solid matrix matrix pH value 5-8 contains
Water 50-75%.
The solid matrix is by 75 parts of citrus peel residues, 25 parts of grass class crop materials, 0.15 part of calcium lime powder mixing system
At.
Crop material is the one kind or mixed for including rice straw, wheat stalk, rape stalk, corn stover or broomcorn straw
Close object.
Stating thallus viable count is 7.1*109cfu/g。
The preparation method of the tearing wax pore fungi solid fungicide, comprising the following steps:
1) tearing wax pore fungi HG2011 strain is taken, punching is seeded in PDB sterilizing culture in the ratio that 100ml is inoculated with 2 fungus blocks
In base；28 DEG C, 150rpm culture 7d, obtain strain mother liquor A；
2) seed culture medium is prepared, strain mother liquor A is inoculated in the seed culture fluid after sterilizing by sterilizing, through liquid fermentation,
Prepare tearing wax pore fungi HG2011 primary seed solution B；
3) solid fermentation culture medium is packed into edible mushroom cultivating bag, high-temperature sterilization, every bag of injection 1L first order seed after cooling
Liquid B, cultivates 24d by 28 DEG C, prepares tearing wax pore fungi HG2011 solid fungicide C；
4) 75 parts of citrus peel residues, 25 parts of grass class crop materials, 0.15 part of calcium lime powder are mixed to the base of system with mass ratio
Matter controls water content of substrate 65%, is inoculated with tearing wax pore fungi HG2011 solid fungicide C according to mass ratio 5%.Fermentation temperature is set
28 DEG C, ferment 28d, and tearing wax pore fungi solid fungicide is prepared.
Agricultural planting application of the tearing wax pore fungi solid fungicide in prevention capsicum epidemic disease.
Agricultural planting application of the tearing wax pore fungi solid fungicide in prevention cucumber blight dis-ease.
The method have the benefit that:
The present invention tears wax pore fungi HG2011 feature, and tearing wax pore fungi HG2011 solid fungicide has significant growth-promoting, increasing
The effect for producing and improving quality is greater than 8% to cucumber and capsicum rate of growth, significantly improves the content of the nutritive indexes such as vitamin C；
The present invention tear wax pore fungi HG2011 solid fungicide under the premise of reducing chemical fertilizer application, reached raising cucumber with
The effect of yield of hot pepper and quality；
The core material citrus peel residue of matrix of the present invention is faintly acid, is to be capable of the suitable of scale acquisition on a large scale well
Preferably tear the raw material of wax pore fungi growth；Citrus peel residue includes a large amount of celluloses, hemicellulose and pectin simultaneously, exactly tearing wax hole
Nutritional ingredient required for bacterium grows；
The core material citrus peel residue of matrix of the present invention is the waste of orange juice factory, and moisture content is high, pollution is big, nothing
Evilization processing difficulty is big, is developed the pollution sources for not only eliminating orange juice factory for tearing wax pore fungi solid fungicide, improved
Economic benefit, it is often more important that opened up the new way for producing a kind of growth-promoting diseases prevention；
The present invention tears wax pore fungi HG2011 solid fungicide to the preventive effect of cucumber blight dis-ease 70% or more, to capsicum epidemic disease
Preventive effect be greater than 70%, therapeutic effect be greater than 50%, that is, overcome chemical fertilizer and chemistry security and environment protecting not
Good drawback also complies with national " weight-reducing subtracts medicine " Strategic Demand.
The present invention tears wax pore fungi HG2011 this solid microbial of solid fungicide than liquid bacterial agent convenient for storage, fortune
It is defeated, solve the problems, such as tearing wax hole bacteria agent commercialization.
Specific embodiment
Example 1 tears the preparation of wax pore fungi HG2011 strain
1. actication of culture: preparing 600ml PDB culture medium (PDB culture medium: potato 200g, glucose with 1L triangular flask
20g, water 1L, pH 7.0), high-temperature sterilization (121 DEG C, 1.5kPa, sterilize 30min)；It goes bail for and is stored in -20 DEG C of tearing wax pore fungi
HG2011 strain, punching are inoculated with 12 fungus blocks (Φ 6mm) and (are inoculated with 2 fungus blocks by 100ml) in 600ml PDB sterilising medium；28
DEG C, 150rpm cultivate 7d, obtain (1ml > 10 strain mother liquor A10cfu)。
2. prepared by seed liquor: preparing 35L seed culture medium (the corn flour 10g sieved with 100 mesh sieve, yeast powder 1g, water 1L, pH
6.5-7.0) in 50L fermentor, sterilizing；600ml strain mother liquor A is inoculated in the seed culture fluid after sterilizing, is sent out through liquid
Ferment (temperature: 27 ± 1 DEG C；Stirring rate: 150rpm；Ventilatory capacity: 10mL L-1min-1；Fermentation time: 5d), prepare tearing wax
(1ml > 10 pore fungi HG2011 primary seed solution B12cfu)。
3. prepared by original seed solid fungicide: taking 1kg solid fermentation culture medium (husk, corn flour, vermiculite, 15 ﹕ in mass ratio, 3 ﹕
15) edible mushroom cultivating bag, ventilated membrane sealing, high-temperature sterilization (121 DEG C, 1.5kPa, 30min), are packed into；Every bag of injection 1L after cooling
Primary seed solution B, cultivates 24d by 28 DEG C, prepares tearing (1g > 10 wax pore fungi HG2011 solid fungicide C10cfu)。
Tearing wax pore fungi HG2011 solid matrix screening test of the example 2 based on citrus peel residue
1, crop material Screening matrix is tested
Respectively based on husk, corn stover, wheat stalk, rape stalk, rice straw and broomcorn straw matrix (with
Mass ratio meter, account for 98%) cooperate 2% corn flour, water content of substrate 60%, access weight ratio 5% tear wax pore fungi
HG2011 solid fungicide C (similarly hereinafter), ferment 28d.It was found that with the processing of+2% corn flour of 98% corn stover or broomcorn straw,
The viable count highest for tearing wax pore fungi HG2011, is 4.2*10 respectively9cfu/g、4.5*109Cfu/g, it is contemplated that corn stover is come
Source is wide, inexpensive, therefore selects corn stover as the raw material of allotment citrus peel residue.
2, citrus peel residue is the solid matrix screening test of primary raw material
5 levels are arranged in citrus peel residue, are 60%, 65%, 70%, 75% and 80% respectively, corn stover (98% corn
+ 2% corn flour of stalk, similarly hereinafter) 5 levels of corresponding citrus peel residue, mixed-matrix water content are 60%, access weight ratio 5%
Wax pore fungi HG2011 solid fungicide C is torn, by solid fermentation 28d, with the proportion of+25% corn stover of 75% citrus peel residue,
Its viable count highest for tearing wax pore fungi HG2011 is 5.4*109cfu/g。
3, mixed-matrix water content screening test
Using+25% corn stover of 75% citrus peel residue as preferred substrate, setting 55%, 60%, 65%, 70%, 75%
Water content accesses 5% tearing wax pore fungi HG2011 solid fungicide C, by solid fermentation 28d, with water content of substrate 65%
Scheme tears the viable count highest of wax pore fungi, is 6.5*109cfu/g。
4, mixed-matrix is suitable for the test of acid or alkali environment
Using+25% corn stover of 75% citrus peel residue as preferred substrate, water content of substrate 65% is respectively set and adds quick lime
Powder 0,0.05%, 0.1%, 0.15%, 0.2% and 0.25%, totally 6 processing, are repeated 4 times.The result shows that add 0.15% life
The tearing wax pore fungi viable count highest of pulverized limestone is 6.1*109cfu/g。
5, the strain amount screening test of wax pore fungi HG2011 is torn
Using+25% corn stover of 75% citrus peel residue as preferred substrate, 0.15% calcium lime powder, water content of substrate is added
65%.Inoculum concentration (solid fungicide C) processing of 4 tearing wax pore fungis, respectively 3.0%, 5%, 7.5%, 10% (weight are set
Than).From 21d, at interval of 3d, the viable count of each processing is measured.Wherein, 3.0% to connect bacterium number 39d viable count up to standard,
7.5% and 10% to connect bacterium amount up to standard in 21d, and 5% to connect bacterium amount in 24d viable count be 6.7*108cfu/g.Comprehensively consider with
5% bacterium amount that connects is preferred dose.
6, wax pore fungi solid fungicide fermentation temperature screening test is torn
Using+25 parts of corn stovers of 75 parts of citrus peel residues as preferred substrate, 0.15 part of calcium lime powder, water content of substrate is added
65%, inoculum concentration 5%.Setting fermentation temperature has: 20,25,28,32,36 DEG C, totally 5 processing, ferment 28d.Wherein, with fermentation
Its viable count highest of 28 DEG C of temperature is 7.1*109cfu/g。
Embodiment 3 tears wax pore fungi solid fungicide application test
1, influence of tearing wax pore fungi solid fungicide (CLA) to cucumber yield and quality
It tests and is carried out in the 4-7 month in 2018 Southwest University experimental plot (have a rest horse in Beibei, chongqing area).It is purple for examination soil
Color soil, middle earth, pH 7.3, organic matter 19.88gkg-1, full nitrogen 0.98gkg-1, full phosphorus 0.92gkg-1, full potassium
19.97g·kg-1, alkali-hydrolyzable nitrogen 60.60mgkg-1, available phosphorus 29.12mgkg-1, effective potassium 53.49mgkg-1.Organic fertilizer
For organic commercial fertilizer (fresh base) organic matter 30.01%, nitrogen content 1.38%, phosphorus content 1.15%, potassium content 1.37%.Test is set
It sets (1) control (CK): not applying fertilizer, base applies the CLA of 50kg/ mus of high pressure steam sterilizations；(2) it conventional fertilizer application (CF): applies respectively per acre
With organic fertilizer, 380,26.6,3.1 and 26.5kg of urea, calcium superphosphate and potassium sulfate, (in terms of N 30% comes from organic fertilizer and 70%
From chemical fertilizer), wherein organic fertilizer, phosphate fertilizer all make base manure, and base applies the CLA of 50kg/ mus of high pressure steam sterilizations, nitrogen potassium fertilizer
10% makees base manure, and remaining 90% is divided into 3 parts, is watered and imposes after flowering stage, bear fruit phase and the harvesting of first result；
(3) conventional fertilizer application+base applies 50kg/ mus of CLA (CF+CLA10): simultaneously base applies 50kg/ mus of CLA to conventional fertilizer application；(4) 75% routine is applied
Fertilizer+base applies 50kg/ mus of CLA (75%CF+CLA10): base manure, top dressing reduce 25% than conventional fertilizer application and base applies 50kg/ mus of CLA.
3500 plants/acre of planting density, random district's groups arrangement is repeated 3 times, Routine Management.Cucumber fruits are harvested in batches, add up fruit number and production
Amount.Best fruiting period fruit 10 are taken, uses anthrone colorimetry, Coomassie brilliant blue G250 method, ninhydrin colorimetry, 2,6-Dichlorophenols respectively
Indophenols titration and determined by ultraviolet spectrophotometry soluble sugar, soluble protein, free amino acid, vitamin C and nitrate
Content.
By Tables 1 and 2 it is found that compared with conventional fertilizer application (CF), application tearing wax pore fungi solid fungicide (CLA) is significantly improved
Per plant number, yield and the free amino acid of cucumber plant, amplification are followed successively by 11.51% -13.61%, 11.71% -
13.87%, 54.37% -71.54%, lose weight and the nitrate content for applying CLA processing (75%CF+CLA) is substantially less than normal
Rule fertilising (CF), the range of decrease 14.93%.In addition, the yield and the index of quality of CF+CLA and 75%CF+CLA are without significant difference.It says
Bright application tearing wax pore fungi solid fungicide makes cucumber yield increase production 10% or more, while improving free aminoacid content and reducing
Nitrate content, quality be improved significantly.
Table 1 tears the influence of wax pore fungi solid fungicide (CLA) to cucumber yield
Table 2 tears the influence of wax pore fungi solid fungicide (CLA) to cucumber quality
2, control efficiency of tearing wax pore fungi solid fungicide (CLA) to cucumber blight dis-ease
Pot experiment is carried out in It In Beibei, Chongqing Southwest University resource environment institute greenhouse in 6-Augusts in 2017,
It is purple soil for examination soil, air-dries, the levigate 2mm that crosses is sieved, spare after formaldehyde fumigation.Physiochemical properties of soil: middle earth, pH
6.5, organic matter 22.32gkg-1, full nitrogen 0.84gkg-1, full phosphorus 0.65gkg-1, full potassium 18.04gkg-1, alkali-hydrolyzable nitrogen
62.81mg·kg-1, available phosphorus 12.20mgkg-1, effective potassium 76.76mgkg-1.Every basin fills soil 1kg, and every basin applies 2g
15-15-15 compound fertilizer mixes, 1 plant of health of every basin kind, uniform 9 age in days cucumber seedling.4 kinds of processing of test setting: (1)
Control (CK): sterile water is sprayed on normal cucumber seedling；(2) germ spore liquid (PI) is sprayed: with 1 × 104CFU/mL cucumber didymella bryoniae
Spore suspension squirts seedling；(3) chemical pesticide+germ (TM+PI): first squirting seedling with 800 times of dilutions of thiophanate methyl,
Blight dis-ease germ is sprayed after for 24 hours again；(4) CLA+ germ (CLA+PI): in field planting, every plant of base applies 10g CLA around root system, with
(2) it handles with (3) while spraying blight dis-ease germ.In addition to " CLA+PI " processing, also in field planting, application 10g goes out for remaining 3 processing
CLA after bacterium.30 basins of each processing, are repeated 3 times, 360 basin, Routine Management totally, and observation stops when the disease incidence of PI is more than 30%
It only tests, investigation plant disease incidence, disease index and control efficiency, while measuring the relative conductivity and third of maximum expansion blade
Dialdehyde content.
Disease index and protection effect are calculated with blade extent of injury.Degree of disease is divided into 5 grades, and 0 grade: without illness；1
Grade: blade is infected area < 10%；2 grades: blade is infected area 10% -25%；3 grades: blade is infected area and is existed
25% -50%；4 grades: blade is infected area 50% -75%；5 grades: blade is infected area > 75%.Disease index=
100 × ∑ [(morbidity number × grades at different levels represent number)/(investigation sum × 5)]；Control efficiency (%)=100 × (the control state of an illness
Index-processing disease index)/control disease index.
Seen from table 3, inoculation muskmelon spherical cavity bacterium (PI) makes melon seedling that blight dis-ease occur.Compared with PI, TM (thiophanate methyl)
The disease index and disease incidence of processing reduce by 29.02 and 26.67%, the disease of CLA (tearing wax pore fungi solid fungicide) processing respectively
Feelings index and disease incidence reduce by 30.60 and 26.67% respectively, and the control efficiency of TM and CLA are without significant difference (75.57% He
79.69%).
Table 3 tears wax pore fungi solid fungicide (CLA) to the control efficiency of cucumber blight dis-ease
3, influence of the tearing wax pore fungi solid fungicide to yield of hot pepper quality
Capsicum variety: ' two early son ' green peppers buyings in seeds company, Bei Bei bureau of agriculture, Chongqing City, 45000 plants of planting density/
ha。
Field trial is in the 4-8 month in 2018 (E106 ° 18 ', N29 ° of the It In Beibei, Chongqing town Xie Ma Southwest University farm
43 ') it carries out.Purple loam, pH 7.3, organic matter 19.88gkg-1, full nitrogen 0.98gkg-1, full phosphorus 0.92gkg-1, entirely
Potassium 19.97gkg-1, alkali-hydrolyzable nitrogen 63.60mgkg-1, available phosphorus 29.12mgkg-1, effective potassium 53.49mgkg-1.Test
Processing: (1) do not apply fertilizer (CK)；(2) conventional fertilizer application (CF)；(3) conventional fertilizer application+50kg/ mus of CLA (CF+CLA)；(4) 75% is conventional
Fertilising+every plant of base applies 50kg/ mus of CLA (75%CF+CLA).In CF processing, N, P are applied respectively per acre2O5、K2O15,10 and
15kg, and base applies the CLA of 50kg/ mus of high pressure steam sterilizations；Wherein, organic N: chemical fertilizer N=3:7, apply organic fertilizer after it is insufficient
Phosphorus potassium is supplied with chemical fertilizer；Organic fertilizer, phosphate fertilizer all make base manure；Residual nitrogen, potash fertilizer press 15%, 15% in flowering stage and first respectively
It is watered and imposes after secondary result harvesting.Plot area 18m2(length × wide=9.0m × 2.0m), every cell plant 36 plants, random district's groups
Arrangement, is repeated 4 times, Routine Management.
Measurement item: Several harvests pepper fruit, record fruit number, single fruit weight and yield.Take the eggplant fruit 50 in the best fruiting period
It is a, respectively with anthrone colorimetry, Coomassie brilliant blue G250 method, ninhydrin colorimetry, 2,6- dichloropheno-lindophenol titration and ultraviolet
Spectrophotometry measures soluble sugar, soluble protein, free amino acid, vitamin C and nitrate content.Fruit harvesting finishes
Afterwards, plant, 105 DEG C of water-removings are harvested, 60 DEG C of drying weigh biomass.
As seen from Table 4, on the basis of chemical fertilizer, application tearing wax pore fungi solid fungicide increases the biology of capsicum significantly
And economic flow rate, the yield ratio CF processing of capsicum increase 10.2%, weight-reducing processing, which is matched, applies tearing wax pore fungi solid fungicide volume increase
8.82%.
As seen from Table 5, chemical fertilizer significantly improves the ascorbic content of capsicum with tearing wax pore fungi solid fungicide is applied, especially
It is weight-reducing with the processing for applying tearing wax pore fungi solid fungicide, soluble protein, free amino acid, soluble sugar and the dimension of capsicum
The content of raw element C is significantly increased.Illustrate that application tearing wax pore fungi solid fungicide significantly improves the nutritional quality of capsicum.
Table 4. tears the influence of wax pore fungi solid fungicide (CLA) to yield of hot pepper
Table 5. tears the influence of wax pore fungi solid fungicide (CLA) to Quality of Capsicum
4, Biocontrol Effect (pot experiment) of the tearing wax pore fungi solid fungicide to Phytophthora capsici
It tests and is carried out in the 5-7 month in 2018 in Southwest University greenhouse.
For trying strain: Phytophthora capsici (P.capsici), is provided by plant protection institute, Southwest University, trained with oat solid
25 DEG C of 3~5d of culture of base are supported, with aseptic water washing spore, and are diluted to 1 × 104CFU·mL-1Spore suspension is used for disease control
Test.
For examination pesticide: " auspicious all " mandipropamid (Mandipropamid) suspending agent, active constituent content 23.4%,
It is purchased from Chongqing means of agricultural production pesticide Co., Ltd, is formulated as 0.34mLL respectively according to operation instruction-1Solution.
Capsicum variety: ' two early son ' green pepper buyings are in seeds company, Bei Bei bureau of agriculture, Chongqing City.
Vegetable field topsoil is acquired, sundries is picked, is air-dried, 3mm sieve is crossed.Every kilogram of soil adds 0.2g urea (N content
46%), 0.4g calcium superphosphate (P2O5Content 12%), 0.2g potassium sulfate (K2O content 50%), loam mixes.It takesPolypots, every alms bowl fill soil 3.0kg.Before and after Hot Pepper Seedling is inoculated with germ, application tears wax pore fungi respectively
HG2011 microbial inoculum (CLA) and chemical pesticide (CP) simulation treatment and prevention etc..Test process: (1) it compares: not being inoculated with germ
(CK)；(2) it is inoculated with germ (Pathogen inoculation, PI): every plant of Hot Pepper Seedling 10mL germ spore liquid seedbed pouring root,
It is transplanted after 1d；(3) CLA treatment processing (PI+CLA): Hot Pepper Seedling in spite of illness is transplanted, every plant of base applies 25g CLA around root system；
(4) CLA prevention processing (CLA+PI): transplant health Hot Pepper Seedling, every plant of base apply 25g CLA around root system, and germ spore is used after 2d
Sub- suspension 10mL/ plants of pouring roots；(5) Hot Pepper Seedling in spite of illness, every plant of application 50mL chemical pesticide treatment processing (PI+CP): are transplanted
Mandipropamid dilution；(6) chemical pesticide prevention processing (CP+PI): transplant health Hot Pepper Seedling, every plant of application 50mL dilution
500 times of mandipropamid dilution uses 10mL/ plants of pouring roots of germ spore suspension after 2d.30 alms bowls of every processing, every alms bowl plantation are peppery
1 plant of green pepper seedling, random arrangement is repeated 4 times, and is managed in the usual way.
According to " capsicum epidemic disease observes and predicts investigation specifications " method (NY/T2060.1-2011), it is spaced 5d investigation plant epidemic disease disease
Evil stops test when the disease incidence of PI processing is more than 50%, calculates disease incidence, disease index and control efficiency:
Disease index=(state of an illness strain number × represent series)/(strain number summation × highest disease grade value) × 100,
Disease incidence (%)=diseased plant number/total strain number × 100,
Control efficiency (%)=(control disease index-processing disease index)/control disease index × 100.
The disease index grade scale of capsicum epidemic disease are as follows:
0 grade: complete stool is disease-free；
1 grade: the blade below of complete stool 10% has a small amount of scab；
2 grades: the blade of complete stool 11%~30% has a small amount of scab or 10% blade below to have a large amount of scabs；
3 grades: the blade morbidity of complete stool 31%~50% or 30% blade below are withered；
4 grades: the blade morbidity of complete stool 51%~75% or 50% blade below are withered, rotten；
5 grades: the blade morbidity of 75% or more complete stool or basal part of stem rot, lodge.
As seen from Table 6, tearing wax pore fungi solid fungicide is to the prevention effect of Phytophthora capsici and the effect of chemical pesticide (CP)
It is close, it is 85.5% (CP) and 78.7% (CLA) respectively；In terms of the treatment to Phytophthora capsici, chemical pesticide (CP) is
72.4%, tearing wax pore fungi solid fungicide is 56.4%, lower than the effect of chemical pesticide.
Table 6 tears wax pore fungi solid fungicide (CLA) to the prevention and treatment effect of Phytophthora capsici
Claims (9)
1. a kind of tearing wax pore fungi solid fungicide, the tearing wax pore fungi solid fungicide is made of viable bacteria and solid matrix, special
Sign is: the viable bacteria is tearing wax pore fungi HG2011, and the thallus viable count of viable bacteria is 3.8*108cfu/g-7.1*109cfu/
g。
2. according to right want 1 described in tearing wax pore fungi solid fungicide, rise be characterized in that: the solid matrix main component is
Citrus peel residue and crop material composition, solid matrix matrix pH value 5-8, water content 50-75%.
3. according to right want 1 described in tearing wax pore fungi solid fungicide, rise be characterized in that: the solid matrix is by 75 portions of citruses
Skin slag, 25 parts of grass class crop materials, 0.15 part of calcium lime powder are mixed.
4. according to right want 2 described in tearing wax pore fungi solid fungicide, rise be characterized in that: crop material be include rice, wheat,
Rape, corn or one kind of sorghum or mixture.
5. according to right want 4 described in tearing wax pore fungi solid fungicide, rise be characterized in that: crop material addition 2% corn flour
Carbon source as crop material.
6. according to right want 1 described in tearing wax pore fungi solid fungicide, rise be characterized in that: the thallus viable count be 7.1*
109cfu/g。
7. the preparation method of tearing wax pore fungi solid fungicide according to claim 1, rises and is characterized in that: including following step
It is rapid:
1) tearing wax pore fungi HG2011 strain is taken, punching is seeded in PDB sterilising medium in the ratio that 100ml is inoculated with 2 fungus blocks
In；28 DEG C, 150rpm culture 7d, obtain strain mother liquor A；
2) seed culture medium is prepared, strain mother liquor A is inoculated in the seed culture fluid after sterilizing by sterilizing, through liquid fermentation, preparation
Wax pore fungi HG2011 primary seed solution B is torn out；
3) solid fermentation culture medium is packed into edible mushroom cultivating bag, high-temperature sterilization, it is cooling after every bag of 1 L primary seed solution B of injection,
28 DEG C, 24d is cultivated, prepares tearing wax pore fungi HG2011 solid fungicide C；
4) 75 parts of quality citrus peel residues, 25 parts of grass class crop materials, 0.15 part of calcium lime powder are mixed to the base of system with mass ratio
Matter controls water content of substrate 65%, according to 5% inoculation tearing wax pore fungi HG2011 solid fungicide C of matrix quality.Setting fermentation
28 DEG C of temperature, ferment 28d, and tearing wax pore fungi solid fungicide is prepared.
8. tearing wax pore fungi solid fungicide as claimed in any one of claims 1 to 6 is answered in the agricultural planting of prevention capsicum epidemic disease
With.
9. tearing wax pore fungi solid fungicide as claimed in any one of claims 1 to 6 is answered in the agricultural planting of prevention cucumber blight dis-ease
With.
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| CN111925948A (en) * | 2020-08-14 | 2020-11-13 | 四川金珠生态农业科技有限公司 | Preparation method and application of high-concentration spore liquid of Ceriporia lacerata |
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| CN106497819A (en) * | 2016-09-18 | 2017-03-15 | 华中农业大学 | Insecticide probiotic bacteria and feed additive |
| CN107164245A (en) * | 2017-07-14 | 2017-09-15 | 西南大学 | One plant tear wax pore fungi growth-promoting functions and its application |
| CN107201317A (en) * | 2017-07-14 | 2017-09-26 | 西南大学 | One plant of tear wax pore fungi and its application for preventing and treating crop fungal disease |
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| CN106497819A (en) * | 2016-09-18 | 2017-03-15 | 华中农业大学 | Insecticide probiotic bacteria and feed additive |
| CN107164245A (en) * | 2017-07-14 | 2017-09-15 | 西南大学 | One plant tear wax pore fungi growth-promoting functions and its application |
| CN107201317A (en) * | 2017-07-14 | 2017-09-26 | 西南大学 | One plant of tear wax pore fungi and its application for preventing and treating crop fungal disease |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111925948A (en) * | 2020-08-14 | 2020-11-13 | 四川金珠生态农业科技有限公司 | Preparation method and application of high-concentration spore liquid of Ceriporia lacerata |
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