CN104542716A - Novel biological seed coating agent and preparation method thereof - Google Patents
Novel biological seed coating agent and preparation method thereof Download PDFInfo
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- CN104542716A CN104542716A CN201410840420.0A CN201410840420A CN104542716A CN 104542716 A CN104542716 A CN 104542716A CN 201410840420 A CN201410840420 A CN 201410840420A CN 104542716 A CN104542716 A CN 104542716A
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Abstract
The invention relates to a novel biological seed coating agent. The biological seed coating agent comprises components in parts by weight as follows: 15-30 parts of bacterial powder and 70-85 parts of a seed coating agent aid, wherein the bacterial powder comprises bacillus mucilaginosus, bacillus amyloliquefaciens and bacillus laterosporus in the weight part ratio of (1-3):(2-4):(2-4); the seed coating agent aid comprises components in parts by weight as follows: 40-60 parts of humic acid, 0.5-5 parts of Pulullan, 15-30 parts of polyvinyl alcohol, 0.05-1 part of a surfactant and 0.1-0.5 parts of a vigilant dye. The novel biological seed coating agent has good film-forming and slow-release functions, has higher film-forming strength, water-absorbing and water-resistant performance and chemical stability, has good biocompatibility with plants and cannot affect the germination rate of seeds; the seed coating agent prepared from bacterial powder of the bacillus mucilaginosus, the bacillus amyloliquefaciens and the bacillus laterosporus has effects of preventing and treating crop diseases and insect damage and promoting plant growth.
Description
Technical field
The present invention relates to coating agent for seed field, particularly a kind of new bio coating agent for seed and preparation method thereof.
Background technology
Seed pelleting take seed as carrier, take coating equipment in sugar production line as means, by the even coated processing treatment technology to the surface of the seed effectively by a certain percentage of the seed coat agent (being generally glue suspension) containing active ingredients such as agricultural chemicals, fertilizer, growth regulators, a seed treatment new technology that Application comparison is general in the world growing up on the basis of tradition seed soaking, seed dressing, be the modernization of seeds processing, the standardization of seed quality and seedling stage field integrated pest management combination.Seed coating technology to improving seeding ratio, control crops seedling stage diseases and pests successful, and has effects such as promoting growth of seedling, increase yield, saving agricultural chemicals and seed consumption, minimizing field medicine fertilizer input and farmland ecological environment pollution.
In seed coat agent, active substance is main mainly with chemical pesticide, is once once employing the chemical pesticide of some high malicious high residues, as Furadan, gram bacterium spirit etc.This brought human safety issues, also to environment both to production and user.Biological seed coating agent is to have the microbial bacterial agent of Biological control function for main component, utilize the occupy-place biological and ecological methods to prevent plant disease, pests, and erosion principle of " with bacterium bacterium ", form a large amount of profitable strain at plant root, the object suppressing and kill multiple harmful levels of pathogens, antagonism crop multiple diseases can be reached; Simultaneously, the heat that viable bacteria breeding generates improves ground temperature, be conducive to the bundle soil of the loose of soil and root system of plant, the material that secretion produces can stimulate growing of crop to a certain extent, and improve the physicochemical property of soil, promote the decomposition of soil indissoluble nutrient, accelerate crop to the absorption of nutrient.Therefore, biological seed coating agent has the advantages that to promote plant growth, improve crop anti-adversity, nontoxic, non-environmental-pollution.
The high-efficiency low-toxicity seed coat agent of biotype has become the focus of a lot of people concern, but development is slower.At present approve to a certain extent, and to put into production and the Bio-seed Coating agent of applying mainly contains rhizobium seed coat agent, for legume seed pelleting; By the seed coat agent that reesei spores is produced, for various vegetables seed pelleting, reach the effect of anti-symptom seedling; The paddy rice seed coating agent that Zhejiang Seed Corp. produces with bacillus subtilis zSB system bacterial strain, for preventing and treating the disease in raising rice seedlings.
The composition of non-active ingredient directly affects quality and the coating effect of seed coat agent, wherein film forming agent is the component of most critical, it ensures that seed coat agent has the key component of active component slowly-releasing in certain viscosity, good film forming, suitable clothing film fastness and the uniformity, particularly clothing film.Desirable coating polymer should possess the features such as film forming after water-soluble, low viscosity, high solid concentration, hydrophilic-hydrophobic sexual balance and drying is firm.At present, not containing the synthetic high polymer class of toxic residua, if poly-amyl acetate, polyacrylamide etc. and natural high molecular substance are as sodium alginate, chitin and derivative thereof etc., be apply more filmogen.
The characteristic that the water penetration required due to film forming agent and resistance to water etc. are conflicting, current most film forming agents can not have the advantage that biocompatibility is strong and film strength is high concurrently.The poor biocompatibility of film forming agent, then can not build the microenvironment being suitable for seed vital movement, has a certain impact to the sprouting of seed and growth of seedling; Film strength is low, then easily come off after dressing, can not play the due effect of seed pelleting.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, one is provided to have good filming and slow-release function, there is higher film strength and absorb water resistance to water and chemical stability, good biocompatibility is had, new bio coating agent for seed not affecting seed germination rate and preparation method thereof with crops.
The present invention solves its technical problem and is achieved through the following technical solutions:
A kind of new bio coating agent for seed, is characterized in that: its composition and parts by weight as follows:
Bacterium powder 15-30 weight portion;
Seed coat agent auxiliary agent 70-85 weight portion;
Wherein, bacterium powder is made up of colloid bacillus cereus, bacillus amyloliquefaciens and the bacillus laterosporus ratio of weight and number by 1-3:2-4:2-4;
Wherein, the composition of seed coat agent auxiliary agent and parts by weight as follows:
Described surfactant is phenethyl phenol polyoxyethyl ether, alkane CF polyoxyethyl ether or calcium dodecyl benzene sulfonate.
A preparation method for new bio coating agent for seed, is characterized in that: the step of this preparation method is:
(1) colloid bacillus cereus, bacillus amyloliquefaciens, bacillus laterosporus are carried out liquid fermentation, then adopt common centrifugal or press spray drying tower spraying dry acquisition bacterium powder, in bacterium powder, living spores number is 10 × 10
8-1000 × 10
8cfu/g;
(2) bacterium powder and seed coat agent auxiliary agent are mixed in proportion, seed coat agent auxiliary agent is mixed in proportion by humus, pulullan polysaccharide, polyvinyl alcohol, surfactant, alertness dyestuff and forms, obtained coating agent for seed finished product after mixing.
Advantage of the present invention and beneficial effect are:
1, new bio coating agent for seed of the present invention, using pulullan polysaccharide polysaccharide as seed coat agent film forming agent, adds colloid bacillus cereus, bacillus amyloliquefaciens and bacillus laterosporus bacterium powder, makes a kind of biological seed coating agent.Pulullan polysaccharide polysaccharide (Pullulan) is a kind of viscosity exocellular polysaccharide secreted by Aureobasidium pullulans (Aurebasidium pullulans) thalline.Pulullan polysaccharide polysaccharide is a kind of polymer substance of colourless, tasteless, odorless, and safety non-toxic edible, low heat value, acid and alkali-resistance, plasticity is good, good film-forming property, and book film gas barrier properties is good, can be degraded by microorganisms, environmental friendliness.
2, new bio coating agent for seed of the present invention, colloid bacillus cereus used is also known as silicate bacterium (Bacillus mucilaginosus Krassilnikov, AS 1.910), its key property is potassium, the silicon that can decomposite in the mineral such as feldspar, mica, also the phosphorus in apatite can be decomposited, and secrete plant growth substance and multiple enzyme, to strengthen the resistance of crop to some diseases.
3, new bio coating agent for seed of the present invention, bacillus amyloliquefaciens used, (Bacillus amyloliquefaciens (Fukumoto) Priest et al., AS 1.1099) be a kind of aerobic sporiferous Gram-positive bacillus, by producing low-molecular-weight antibiotic and antiseptic protein polypeptide isoreactivity material suppresses pathogen and nematode.The extracellular enzyme that bacillus amyloliquefaciens produces mainly contains phytase, chitinase, dextranase, protease, amylase, rennin etc., main lipopeptid class metabolite has the antagonistic activity materials such as Surfactin (surfcatin), fragrant shepherd's purse element (fengycin), iturin (litarin) and bacillomycin Bacillomy-cinD, produces auxin indole-3-acetic acid (IAA) in addition.
4, new bio coating agent for seed of the present invention, bacillus laterosporus used, (Bacillus laterosporus Laubach, AS 1.864) belong to bacillus, Gram-positive, can be changed into feminine gender, gemma is oval, side is raw, middle life or near middle raw, and sporangiocyst expands, free gemma thicker than another side (canoe shape).Bacillus laterosporus can secrete a large amount of chitinase, Antifungi class disease; To noxious bacterias such as vibrios, Escherichia coli and baculovirals, there is very strong inhibitory action.
5, new bio coating agent for seed of the present invention, there is the purposes of preventing and treating crop pest, insect pest and Promoting plant growth, described crop is Chinese cabbage, cucumber, capsicum, tomato, wheat, paddy rice, cotton, described disease is virus disease, fusarium wilt, gray mold, anthracnose, powdery mildew, downy mildew, epidemic disease, and described insect pest is nematode.
6, new bio coating agent for seed of the present invention, adopt pulullan polysaccharide as the film forming agent of seed coat agent, there is good film forming and slow-release function, there is higher film strength and absorb water resistance to water and chemical stability, with plant, particularly have good biocompatibility with crops, the present invention's pulullan polysaccharide film-forming agent for seed coating used can not affect the eupnea of seed, thus can not affect the germination rate of seed.
Embodiment
The invention will be further described below, and following examples are descriptive, is not determinate, can not limit protection scope of the present invention with this.
Embodiment 1:
Inventor has carried out field trial in Tianjin.Test site is in Tianjin Jinghai County, and test soil fertility is general.The component of the present invention's coating agent for seed used is:
The bacterium powder 30g of 12g is made up of colloid bacillus cereus 6g, bacillus amyloliquefaciens 12g, bacillus laterosporus, humus 50g, pulullan polysaccharide 1g, polyvinyl alcohol 18g, phenethyl phenol polyoxyethyl ether 0.5g, gentian violet 0.5g, obtained seed coating medicine 100g, wrap by 5 kilograms of cotton seeds, natural air drying.
For planting experimentally protonatomic mass testing result: purity >=85%; Germination rate >=85%, water content≤12%; , be above-mentioned seed coat agent for examination A, B chemical agent seed coating medicine, C is blank.
Test adopts randomized arrangement, repeats 3 times, 3 kinds of process, 9 communities, plot area 20m
2.The dressing of medicament prior to seeding 7 days by seed coating medicine: the concentration of seed 1:50 carries out artificial dressing, seed pelleting terminate rear shakeout respectively dry for subsequent use.Before sowing, turn in soil, rakes.Then partition, ridging, sowing.Line-spacing 60-70cm, about spacing in the rows 25cm, every cave program request cotton seeds 4-5 grain, uniform broadcasting 100 surveys emergence rate with process cotton seed.
Emergence rate (%)=number of emerging/sowing seed number × 100
At cotton in seedling stage, choosing 40 strains cotton seedlings investigation cotton seedling blight incidences at random in each community, calculates disease index and protection effect.
Sick seedling grade scale:
0 grade: cotton shoot root portion is without scab;
1 grade: scab accounts for all areas of stem and is less than 1/4;
2 grades: scab is greater than all areas of 1/4 stem and is less than all areas of 1/2 stem;
3 grades: scab is greater than all areas of 1/2 stem and is less than all areas of 3/4 stem;
4 grades: scab is greater than all areas of 3/4 stem.
Protection effect (%)=(contrast disease index-process disease index)/contrast disease index × 100 investigation different disposal seed is on the impact of emergence rate and the cotton seedling blight incidence of disease, as shown in table 1:
Table 1 different disposal emergence rate and diseases prevention situation
| Process | Emergence rate (%) | Disease index (%) | Protection effect |
| A | 91 | 10.5 | 50.5 |
| B | 87 | 15.2 | 28.3 |
| C | 84 | 21.2 |
As seen from Table 1, coating agent for seed emergence rate of the present invention is the highest, improves 8.33% than blank, after seed coat agent process of the present invention, the seedling diseases disease index of cotton is 10.5, significantly lower than the disease index of blank, protection effect is 50.5%, also higher than commercially available chemical agent seed coating medicine.
Embodiment 2:
Inventor has carried out field trial in Tianjin.Test site is in Tianjin Jinghai County, and test soil fertility is general.The component of the present invention's coating agent for seed used is:
The bacterium powder 25g be made up of colloid bacillus cereus 3g, bacillus amyloliquefaciens 10g, bacillus laterosporus 12g, humus 55g, pulullan polysaccharide 1.5g, polyvinyl alcohol 17g, alkane CF polyoxyethyl ether 1g, gentian violet 0.5g, obtained seed coating medicine, 100g wraps by 5 kilograms of cotton seeds, natural air drying.
For planting experimentally protonatomic mass testing result: purity >=85%; Germination rate >=85%, water content≤12%; , be above-mentioned seed coat agent for examination A, B chemical agent seed coating medicine, C is blank.
Test adopts randomized arrangement, repeats 3 times, 3 kinds of process, 9 communities, plot area 20m
2.The dressing of medicament prior to seeding 7 days by seed coating medicine: the concentration of seed 1:50 carries out artificial dressing, seed pelleting terminate rear shakeout respectively dry for subsequent use.Before sowing, turn in soil, rakes.Then partition, ridging, sowing.Line-spacing 60-70cm, about spacing in the rows 25cm, every cave program request cotton seeds 4-5 grain, uniform broadcasting 100 surveys emergence rate with process cotton seed.The computing formula of emergence rate, disease index and protection effect, reference example 1.
Investigate the impact on emergence rate and the cotton seedling blight incidence of disease of different disposal seed, as shown in table 2:
Table 2 different disposal emergence rate and diseases prevention situation
| Process | Emergence rate (%) | Disease index (%) | Protection effect |
| A | 89 | 12.5 | 38.1 |
| B | 86 | 15.6 | 22.8 |
| C | 84 | 20.2 |
As seen from Table 2, seed coating medicine emergence rate of the present invention is the highest, improves 5.95% than blank, after seed coat agent process of the present invention, the seedling diseases disease index of cotton is 12.5, significantly lower than the disease index of blank, protection effect is 38.1%, also higher than commercially available chemical agent seed coating medicine.
Embodiment 3:
Inventor has carried out field trial in Tianjin.Test site is in Tianjin Jinghai County, and test clay fertilizer power is general.The component of the present invention's coating agent for seed used is:
The bacterium powder 20g be made up of colloid bacillus cereus 4g, bacillus amyloliquefaciens 8g, bacillus laterosporus 8g, humus 50g, pulullan polysaccharide 1.5g, polyvinyl alcohol 27g, phenethyl phenol polyoxyethyl ether 1g, gentian violet 0.5g, obtained seed coating medicine 100g, wrap by 5 kilograms of cotton seeds, natural air drying.
For planting experimentally protonatomic mass testing result: purity >=85%; Germination rate >=85%, water content≤12%; , be above-mentioned seed coat agent for examination A, B chemical agent seed coating medicine, C is blank.
Test adopts randomized arrangement, repeats 3 times, 3 kinds of process, 9 communities, plot area 20m
2.The dressing of medicament prior to seeding 7 days by seed coating medicine: the concentration of seed 1:50 carries out artificial dressing, seed pelleting terminate rear shakeout respectively dry for subsequent use.Before sowing, turn in soil, rakes.Then partition, ridging, sowing.Line-spacing 60-70cm, about spacing in the rows 25cm, every cave program request cotton seeds 4-5 grain, uniform broadcasting 100 surveys emergence rate with process cotton seed.The computing formula of emergence rate, disease index and protection effect, reference example 1.
Investigate the impact on emergence rate and the cotton seedling blight incidence of disease of different disposal seed, as shown in table 3:
Table 3 different disposal emergence rate and diseases prevention situation
| Process | Emergence rate (%) | Disease index (%) | Protection effect |
| A | 87 | 14.5 | 28.2 |
| B | 86 | 15.0 | 25.7 |
| C | 84 | 20.2 |
As seen from Table 3, seed coating medicine emergence rate of the present invention is the highest, improves 3.57% than blank, after seed coat agent process of the present invention, the seedling diseases disease index of cotton is 14.5, significantly lower than the disease index of blank, protection effect is 28.2%, also higher than commercially available chemical agent seed coating medicine.
Although disclosed embodiments of the invention for the purpose of illustration, but it will be appreciated by those skilled in the art that, in the spirit and scope not departing from the present invention and claims, various replacement, change and amendment are all possible, and therefore scope of the present invention is not limited to the content disclosed in embodiment.
Claims (3)
1. a new bio coating agent for seed, is characterized in that: its composition and parts by weight as follows:
Bacterium powder 15-30 weight portion;
Seed coat agent auxiliary agent 70-85 weight portion;
Wherein, bacterium powder is made up of colloid bacillus cereus, bacillus amyloliquefaciens and the bacillus laterosporus ratio of weight and number by 1-3:2-4:2-4;
Wherein, the composition of seed coat agent auxiliary agent and parts by weight as follows:
2. new bio coating agent for seed according to claim 1, is characterized in that: described surfactant is phenethyl phenol polyoxyethyl ether, alkane CF polyoxyethyl ether or calcium dodecyl benzene sulfonate.
3. a preparation method for new bio coating agent for seed, is characterized in that: the step of this preparation method is:
(1) colloid bacillus cereus, bacillus amyloliquefaciens, bacillus laterosporus are carried out liquid fermentation, then adopt common centrifugal or press spray drying tower spraying dry acquisition bacterium powder, in bacterium powder, living spores number is 10 × 10
8-1000 × 10
8cfu/g;
(2) bacterium powder and seed coat agent auxiliary agent are mixed in proportion, seed coat agent auxiliary agent is mixed in proportion by humus, pulullan polysaccharide, polyvinyl alcohol, surfactant, alertness dyestuff and forms, obtained coating agent for seed finished product after mixing.
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Cited By (7)
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| CN105010402A (en) * | 2015-07-06 | 2015-11-04 | 领先生物农业股份有限公司 | Suspended biological seed coating agent and preparation method and application thereof |
| CN105494446A (en) * | 2015-12-21 | 2016-04-20 | 云南农业大学 | Biological compound seed coating agent and application |
| CN107333490A (en) * | 2017-09-05 | 2017-11-10 | 江苏省农业科学院 | A kind of pelletized formula of Salt And Alkali Tolerance rapeseed and processing method |
| CN108587986A (en) * | 2018-06-15 | 2018-09-28 | 保定微控生物科技有限公司 | A kind of bacillus amyloliquefaciens with diseases prevention and degrading organic phosphor double action |
| CN109329310A (en) * | 2018-11-28 | 2019-02-15 | 乳山韩威生物科技有限公司 | A kind of silk tree endogenetic bacteria H6 fermentation material suspension seed-coating agent and preparation method thereof |
| CN109757488A (en) * | 2019-02-20 | 2019-05-17 | 广东省农业科学院植物保护研究所 | A kind of microorganism seed pill pelletizing coating composition |
| CN110999923A (en) * | 2019-12-09 | 2020-04-14 | 北京绿昇原生物科技有限公司 | Targeted defense seed coating biological agent and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105010402A (en) * | 2015-07-06 | 2015-11-04 | 领先生物农业股份有限公司 | Suspended biological seed coating agent and preparation method and application thereof |
| CN105010402B (en) * | 2015-07-06 | 2018-06-05 | 领先生物农业股份有限公司 | A kind of suspension type biological seed coat agent, its preparation method and purposes |
| CN105494446A (en) * | 2015-12-21 | 2016-04-20 | 云南农业大学 | Biological compound seed coating agent and application |
| CN107333490A (en) * | 2017-09-05 | 2017-11-10 | 江苏省农业科学院 | A kind of pelletized formula of Salt And Alkali Tolerance rapeseed and processing method |
| CN108587986A (en) * | 2018-06-15 | 2018-09-28 | 保定微控生物科技有限公司 | A kind of bacillus amyloliquefaciens with diseases prevention and degrading organic phosphor double action |
| CN108587986B (en) * | 2018-06-15 | 2021-06-01 | 齐永新 | Bacillus amyloliquefaciens with dual functions of preventing diseases and degrading organic phosphorus |
| CN109329310A (en) * | 2018-11-28 | 2019-02-15 | 乳山韩威生物科技有限公司 | A kind of silk tree endogenetic bacteria H6 fermentation material suspension seed-coating agent and preparation method thereof |
| CN109757488A (en) * | 2019-02-20 | 2019-05-17 | 广东省农业科学院植物保护研究所 | A kind of microorganism seed pill pelletizing coating composition |
| CN110999923A (en) * | 2019-12-09 | 2020-04-14 | 北京绿昇原生物科技有限公司 | Targeted defense seed coating biological agent and preparation method thereof |
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Application publication date: 20150429 |