CN103004885A - Rape biologic seed coating and preparation method and applications thereof - Google Patents
Rape biologic seed coating and preparation method and applications thereof Download PDFInfo
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- CN103004885A CN103004885A CN2012104350429A CN201210435042A CN103004885A CN 103004885 A CN103004885 A CN 103004885A CN 2012104350429 A CN2012104350429 A CN 2012104350429A CN 201210435042 A CN201210435042 A CN 201210435042A CN 103004885 A CN103004885 A CN 103004885A
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- agent
- kind clothing
- brassinosteroid biosynthesis
- rape
- fertilizer
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- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 239000011248 coating agent Substances 0.000 title abstract description 23
- 238000000576 coating method Methods 0.000 title abstract description 13
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 115
- 241000194103 Bacillus pumilus Species 0.000 claims abstract description 45
- 239000003337 fertilizer Substances 0.000 claims abstract description 29
- 230000000443 biocontrol Effects 0.000 claims abstract description 12
- 239000002562 thickening agent Substances 0.000 claims abstract description 11
- 241000221662 Sclerotinia Species 0.000 claims abstract description 8
- 230000012010 growth Effects 0.000 claims abstract description 8
- IXVMHGVQKLDRKH-YEJCTVDLSA-N (22s,23s)-epibrassinolide Chemical compound C1OC(=O)[C@H]2C[C@H](O)[C@H](O)C[C@]2(C)[C@H]2CC[C@]3(C)[C@@H]([C@H](C)[C@H](O)[C@@H](O)[C@H](C)C(C)C)CC[C@H]3[C@@H]21 IXVMHGVQKLDRKH-YEJCTVDLSA-N 0.000 claims description 66
- IXVMHGVQKLDRKH-VRESXRICSA-N Brassinolide Natural products O=C1OC[C@@H]2[C@@H]3[C@@](C)([C@H]([C@@H]([C@@H](O)[C@H](O)[C@H](C(C)C)C)C)CC3)CC[C@@H]2[C@]2(C)[C@@H]1C[C@H](O)[C@H](O)C2 IXVMHGVQKLDRKH-VRESXRICSA-N 0.000 claims description 66
- 230000015572 biosynthetic process Effects 0.000 claims description 66
- 239000012530 fluid Substances 0.000 claims description 31
- 240000002791 Brassica napus Species 0.000 claims description 26
- 235000004977 Brassica sinapistrum Nutrition 0.000 claims description 19
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical group OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 18
- 239000000243 solution Substances 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- 244000005700 microbiome Species 0.000 claims description 13
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 11
- 239000007864 aqueous solution Substances 0.000 claims description 10
- 239000003755 preservative agent Substances 0.000 claims description 10
- 230000002335 preservative effect Effects 0.000 claims description 10
- 238000004040 coloring Methods 0.000 claims description 9
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical compound [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 claims description 8
- 235000010241 potassium sorbate Nutrition 0.000 claims description 8
- 239000004302 potassium sorbate Substances 0.000 claims description 8
- 229940069338 potassium sorbate Drugs 0.000 claims description 8
- 235000006008 Brassica napus var napus Nutrition 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 7
- ULWHHBHJGPPBCO-UHFFFAOYSA-N propane-1,1-diol Chemical class CCC(O)O ULWHHBHJGPPBCO-UHFFFAOYSA-N 0.000 claims description 7
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 claims description 7
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 claims description 6
- 239000001768 carboxy methyl cellulose Substances 0.000 claims description 6
- 239000001103 potassium chloride Substances 0.000 claims description 6
- 235000011164 potassium chloride Nutrition 0.000 claims description 6
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 claims description 6
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 claims description 6
- 229920001285 xanthan gum Polymers 0.000 claims description 6
- 244000215068 Acacia senegal Species 0.000 claims description 5
- 229920000084 Gum arabic Polymers 0.000 claims description 5
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 claims description 5
- 239000004372 Polyvinyl alcohol Substances 0.000 claims description 5
- 239000000205 acacia gum Substances 0.000 claims description 5
- 235000010489 acacia gum Nutrition 0.000 claims description 5
- 229910052748 manganese Inorganic materials 0.000 claims description 5
- 239000011572 manganese Substances 0.000 claims description 5
- 235000007079 manganese sulphate Nutrition 0.000 claims description 5
- 239000011702 manganese sulphate Substances 0.000 claims description 5
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 5
- 229920002451 polyvinyl alcohol Polymers 0.000 claims description 5
- 229920001661 Chitosan Polymers 0.000 claims description 4
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 4
- ABBQHOQBGMUPJH-UHFFFAOYSA-M Sodium salicylate Chemical compound [Na+].OC1=CC=CC=C1C([O-])=O ABBQHOQBGMUPJH-UHFFFAOYSA-M 0.000 claims description 4
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 4
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 claims description 4
- 235000010234 sodium benzoate Nutrition 0.000 claims description 4
- 239000004299 sodium benzoate Substances 0.000 claims description 4
- 229960004025 sodium salicylate Drugs 0.000 claims description 4
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 claims description 3
- 229910021380 Manganese Chloride Inorganic materials 0.000 claims description 3
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 claims description 3
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 3
- 229910052796 boron Inorganic materials 0.000 claims description 3
- 235000006748 manganese carbonate Nutrition 0.000 claims description 3
- 239000011656 manganese carbonate Substances 0.000 claims description 3
- 229940093474 manganese carbonate Drugs 0.000 claims description 3
- 235000002867 manganese chloride Nutrition 0.000 claims description 3
- 239000011565 manganese chloride Substances 0.000 claims description 3
- 229940099607 manganese chloride Drugs 0.000 claims description 3
- 229910000016 manganese(II) carbonate Inorganic materials 0.000 claims description 3
- XMWCXZJXESXBBY-UHFFFAOYSA-L manganese(ii) carbonate Chemical compound [Mn+2].[O-]C([O-])=O XMWCXZJXESXBBY-UHFFFAOYSA-L 0.000 claims description 3
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 3
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 3
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 claims description 3
- 229910052939 potassium sulfate Inorganic materials 0.000 claims description 3
- 239000001120 potassium sulphate Substances 0.000 claims description 3
- 235000011151 potassium sulphates Nutrition 0.000 claims description 3
- 239000011701 zinc Substances 0.000 claims description 3
- 229910052725 zinc Inorganic materials 0.000 claims description 3
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 2
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 2
- 229910052802 copper Inorganic materials 0.000 claims description 2
- 239000010949 copper Substances 0.000 claims description 2
- 229910052742 iron Inorganic materials 0.000 claims description 2
- SWHAQEYMVUEVNF-UHFFFAOYSA-N magnesium potassium Chemical class [Mg].[K] SWHAQEYMVUEVNF-UHFFFAOYSA-N 0.000 claims description 2
- 229940072033 potash Drugs 0.000 claims description 2
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 claims description 2
- 235000015320 potassium carbonate Nutrition 0.000 claims description 2
- 229960002816 potassium chloride Drugs 0.000 claims description 2
- 235000010333 potassium nitrate Nutrition 0.000 claims description 2
- 239000004323 potassium nitrate Substances 0.000 claims description 2
- 230000001737 promoting effect Effects 0.000 claims description 2
- 241000894006 Bacteria Species 0.000 abstract description 3
- 230000008901 benefit Effects 0.000 abstract description 3
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- 238000009776 industrial production Methods 0.000 abstract description 2
- 238000003860 storage Methods 0.000 abstract description 2
- 238000005260 corrosion Methods 0.000 abstract 1
- 230000007797 corrosion Effects 0.000 abstract 1
- 238000000855 fermentation Methods 0.000 abstract 1
- 230000004151 fermentation Effects 0.000 abstract 1
- 230000000361 pesticidal effect Effects 0.000 abstract 1
- 239000003638 chemical reducing agent Substances 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 239000002917 insecticide Substances 0.000 description 5
- 239000006187 pill Substances 0.000 description 5
- 239000002994 raw material Substances 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 239000004480 active ingredient Substances 0.000 description 4
- 230000000844 anti-bacterial effect Effects 0.000 description 4
- 239000003899 bactericide agent Substances 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 239000000843 powder Substances 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 241000238631 Hexapoda Species 0.000 description 3
- 239000004927 clay Substances 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 239000000575 pesticide Substances 0.000 description 3
- 239000011573 trace mineral Substances 0.000 description 3
- 235000013619 trace mineral Nutrition 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 description 2
- 239000005906 Imidacloprid Substances 0.000 description 2
- 239000012752 auxiliary agent Substances 0.000 description 2
- 230000000853 biopesticidal effect Effects 0.000 description 2
- 239000006013 carbendazim Substances 0.000 description 2
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 description 2
- 238000012505 colouration Methods 0.000 description 2
- 239000013530 defoamer Substances 0.000 description 2
- FPAFDBFIGPHWGO-UHFFFAOYSA-N dioxosilane;oxomagnesium;hydrate Chemical compound O.[Mg]=O.[Mg]=O.[Mg]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O FPAFDBFIGPHWGO-UHFFFAOYSA-N 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000000857 drug effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000035784 germination Effects 0.000 description 2
- YWTYJOPNNQFBPC-UHFFFAOYSA-N imidacloprid Chemical compound [O-][N+](=O)\N=C1/NCCN1CC1=CC=C(Cl)N=C1 YWTYJOPNNQFBPC-UHFFFAOYSA-N 0.000 description 2
- 229940056881 imidacloprid Drugs 0.000 description 2
- 231100000053 low toxicity Toxicity 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- 235000019198 oils Nutrition 0.000 description 2
- 238000005453 pelletization Methods 0.000 description 2
- 239000005648 plant growth regulator Substances 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 241000235342 Saccharomycetes Species 0.000 description 1
- 241000221696 Sclerotinia sclerotiorum Species 0.000 description 1
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- 241000607479 Yersinia pestis Species 0.000 description 1
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- 230000000996 additive effect Effects 0.000 description 1
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- 229940088710 antibiotic agent Drugs 0.000 description 1
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- 230000000740 bleeding effect Effects 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
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- 230000007812 deficiency Effects 0.000 description 1
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- 235000013399 edible fruits Nutrition 0.000 description 1
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- NYPJDWWKZLNGGM-UHFFFAOYSA-N fenvalerate Chemical compound C=1C=C(Cl)C=CC=1C(C(C)C)C(=O)OC(C#N)C(C=1)=CC=CC=1OC1=CC=CC=C1 NYPJDWWKZLNGGM-UHFFFAOYSA-N 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
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- ATROHALUCMTWTB-OWBHPGMISA-N phoxim Chemical compound CCOP(=S)(OCC)O\N=C(\C#N)C1=CC=CC=C1 ATROHALUCMTWTB-OWBHPGMISA-N 0.000 description 1
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Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a rape biologic seed coating and a preparation method and applications thereof. According to the rape biologic seed coating, Biocontrol bacterium fermentation liquor is used as an active component; the biocontrol bacterium is bacillus pumilus GBSC66 CGMCC No. 3379; the rape biologic seed coating further comprises a micro element fertilizer, a filmogen solution, a thickener, a corrosion remover, an antifreezing agent and a colorant. The rape biologic seed coating provided by the invention has the advantages of high pesticide effect, security, residue freeness and pollution freeness; after the rape biologic seed coating is used for coating seeds, not only are the growth and the development of the rape significantly prompted, but also the rape sclerotinia rot can be effectively prevented and treated; and meanwhile the rape biologic seed coating provided by the invention is simple in production method, low in cost, convenient to implement and long in storage life, and is applicable to large-scale industrial production.
Description
Technical field
The present invention relates to a kind of biopesticide preparation, specifically, is a kind of for brassinosteroid biosynthesis kind clothing agent and its production and use.
Background technology
Rape is one of important oil crop of China, and its cultivated area accounts for more than 50% of national oil crop total cultivated area.Along with the increase of cultivated area, the rape damage by disease and insect is year by year ascendant trend, causes the yield of rape loss serious.The seed dressing of severe toxicity, persistent pesticide is the main method of preventing and treating for many years the rape seedling damage by disease and insect always, but the long-term use of single chemical pesticide, and so that the damage by disease and insect resistance increases, preventive effect reduces.Cause simultaneously environmental pollution, residual serious, even person poultry poisoning's event occurs.Efficiently, the research and development of low toxicity, the agent of noresidue kind clothing have become problem demanding prompt solution.
Kind of clothing technology is that of growing up on tradition seed soaking, the basis of seed dressing uses commonplace seed processing technology in the world, is the standardization of seed quality, the modernization of seed processing and the combination of field integrated pest prevention and control in seedling stage.Planting the clothing agent is comprised of active component and non-active ingredient usually.Active component mainly comprises insecticide, bactericide, hormone, fertilizer or beneficial microbe and metabolite thereof etc., is the active ingredient that directly plays a role in kind of the clothing agent.Non-active ingredient refers to film forming agent and the corresponding additive in kind of the clothing agent, to keep kind of the physics of clothing agent and a chemical property, the important component of active component slowly-releasing comprises film forming agent, dispersant, suspending agent, antifreezing agent, warning colouration, defoamer, thickener, preservative etc. in the controlling diaphragm.The composition of non-active ingredient directly affects kind of the quality of clothing agent and a coating effect.
CN 102197821A discloses a kind of suspension type rape seed coating agent, it is formulated through wet grinding by film forming agent, emulsifier, antifreezing agent, warning colorant and insecticide, bactericide, plant growth regulator, trace element fertilizer and water, is applicable to all kinds of rape seed Cotton seeds.This invents available Mechanical Method or manual method dressing, and the pharmacopoeia mass ratio is up to 1:200-300; Can form the clothing film with dense film duct at the surface of the seed during dressing, active component can slowly discharge by the fenestra road, and the lasting period reaches 55-70 days.
CN 1537425A discloses a kind of rape pill pelletizing coating draught-resistant seed coating agent and preparation method thereof, is that to be ground into respectively fineness be powder more than 200 orders for white clay with the talcum powder of the water-loss reducer of 0.5%-5%, 1%-10% and 50%-80%; With 0.15%-0.40% insecticide, 0.35%-0.65% bactericide, 0.05%-0.15%KH
2PO
4, to be broken into fineness be the above powder of 250 orders for 5%-40% peat and the even mixed powder of 0.01%-0.03% root-inducing powder; Stir in the input agitator after water-loss reducer after the processing and white clay mix with mixture, obtain rape pill pelletizing coating draught-resistant seed coating agent.
CN 1475117A discloses a kind of coating agent for seed for rape seed.It by auxiliary agent, medicament and water by weight the volumn concentration mixing and stirring form.Auxiliary agent is: film forming agent: shitosan, polyvinyl alcohol and gum Arabic; Surfactant: Triton X-100; Antifreezing agent: ethylene glycol; Bleeding agent: acetone; Colloid protective agent: phenol; Defoamer: tributyl phosphate; Warning colouration: red or other coloured pigment; Medicament is: insecticide: the insecticide of one or both mixing in the same phoxim of Imidacloprid or Imidacloprid, the sumicidin; Bactericide: carbendazim or carbendazim and tmtd; Plant growth regulator: uniconazole P; Trace element: boron fertilizer, manganese fertilizer and zinc fertilizer; Water.
CN 1552177A discloses a kind of rape seed drought-resisting coating pill and pill preparation technology thereof.The water-loss reducer solution of configuration 0.05%-1.0% evenly is sprayed onto water-loss reducer solution on the selected seed, carries out internal layer coating, adds clay powder, forms single seed nuclear; Again draught-resistant seed coating agent is added in the seed, Yi Bian mixing, Yi Bian spray into water-loss reducer solution, carry out the middle level pill.Add a small amount of mistake 200 purpose talcum powder, spray into aposematic water-loss reducer solution, carry out outer dressing, after even kind, the drying, packing.
Biological seed coating agent is the important component part of biopesticide, its active component is natural products, microorganism live body or its metabolite that can control phytopathogen, mainly comprises microbiocidal (living microorganism and farm antibiotics etc.), plant resource type, the formulation of synthesizing take microbial metabolic products as the guide, genetic engineering bacterial strain etc.Study verifiedly, the biological seed coating agent dressing can strengthen seed vitality, improves seedling quality, promotes plant strain growth, improves output, improves crop quality, improve crop resistance.In addition, with respect to the agent of chemical seed clothing, biological seed coating agent has safety, noresidue, the advantage such as pollution-free, pays close attention to widely having obtained various countries in recent years.But, have not yet to see the play-by-play relevant for the agent of brassinosteroid biosynthesis kind clothing.
Summary of the invention
One of the object of the invention is for the deficiencies in the prior art, and the brassinosteroid biosynthesis kind clothing agent of a kind of, low toxicity nuisanceless to environment, safety is provided, and it not only has growth-promoting functions to rape, and can effectively prevent and treat sclerotinia rot of colza.Two of purpose of the present invention is to provide the preparation method of described brassinosteroid biosynthesis kind clothing agent.Three of purpose of the present invention is to provide the using method of described brassinosteroid biosynthesis kind clothing agent.Four of purpose of the present invention is to provide the purposes of described brassinosteroid biosynthesis kind clothing agent.
For one of achieving the above object, the present invention adopts following technical scheme:
The agent of a kind of brassinosteroid biosynthesis kind clothing, its active component is the biocontrol microorganisms zymotic fluid, described biocontrol microorganisms is bacillus pumilus (Bacillus pumilus) GBSC66 CGMCC No.3379, Classification And Nomenclature is bacillus pumilus (Bacillus pumilus) GBSC66, be positioned at the common micro-organisms center preservation of Pekinese China Committee for Culture Collection of Microorganisms on November 2nd, 2009, preserving number is CGMCC No.3379.
Described bacillus pumilus (Bacillus pumilus) GBSC66 CGMCC No.3379 is that loose many grass roots from worker Bu Jiada town, Tibet enclose the suitable living bacterium of the low temperature that separates acquisition the soil in the early-stage Study of the present invention, be proved the effect that has remarkable inhibition Sclerotinia sclerotiorum and saccharomycete and significantly promote growth of rape, had good control efficiency for sclerotinia rot of colza.
Preferably, the concentration of biocontrol microorganisms is 〉=10 in the described biocontrol microorganisms zymotic fluid
7Cfu/ml for example can be 10
7Cfu/ml, 10
8Cfu/ml, 10
9Cfu/ml, 10
10Cfu/ml, 10
11Cfu/ml; More preferably 10
7-10
9Cfu/ml; Most preferably be 10
8Cfu/ml.
Preferably, the mass percent (wt%) of described biocontrol microorganisms zymotic fluid in brassinosteroid biosynthesis kind clothing of the present invention agent is 10wt%-40wt%, for example can be 10wt%-17.5wt%, 13.6wt%-34wt%, 21.2wt%-37wt%, 29wt%-40wt%, 10wt%, 12wt%, 15wt%, 18.3wt%, 20wt%, 22.5wt%, 25wt%, 28.4wt%, 30wt%, 30.6wt%, 33wt%, 35wt%, 36.1wt%, 39wt%, 40wt%; 20wt%-30wt% more preferably; Most preferably be 25wt%.
Further, can also comprise little fertilizer, film forming agent solution, thickener, antifreezing agent, preservative, colouring agent and water in the brassinosteroid biosynthesis kind clothing of the present invention agent.
Described little fertilizer can provide for plant the fertilizer of trace element, can use in the present invention such as copper fertilizer, boron fertilizer, molydbenum fertilizer, manganese fertilizer, iron fertilizer and zinc fertilizer etc. commonly used; Be preferably manganese fertilizer and potash fertilizer, such as manganese sulphate, manganese carbonate, manganese chloride, potassium chloride, potassium sulphate, potassium dihydrogen phosphate, potassium magnesium salts, potassium nitrate etc. are arranged; Most preferably be manganese sulphate and/or potassium chloride.
The mass percent (wt%) of described little fertilizer in brassinosteroid biosynthesis kind clothing of the present invention agent is 2wt%-5wt%, for example can be 2wt%-3.5wt%, 2.3wt%-4wt%, 3.2wt%-4.9wt%, 4.1wt%-5wt%, 2wt%, 2.1wt%, 2.5wt%, 2.8wt%, 3wt%, 3.4wt%, 3.6wt%, 3.9wt%, 4wt%, 4.2wt%, 4.5wt%, 4.6wt%, 4.81wt%, 5wt%; 3wt%-4wt% more preferably; Most preferably be 3.5wt%.
Described film forming agent solution is preferably the mixture of any one or at least two kinds in the aqueous solution that mass concentration is the sodium carboxymethylcellulose of 3wt%, polyvinyl alcohol water solution that mass concentration is 4wt%, the chitosan aqueous solution that mass concentration is 2wt%, most preferably is the aqueous solution that mass concentration is the sodium carboxymethylcellulose of 3wt%.
The mass percent (wt%) of described film forming agent solution in brassinosteroid biosynthesis kind clothing of the present invention agent is 50wt%-70wt%, for example can be 50wt%-53.8wt%, 57.3wt%-64wt%, 60.2wt%-65wt%, 59.1wt%-70wt%, 50wt%, 52.1wt%, 53wt%, 54.5wt%, 55wt%, 56wt%, 58.1wt%, 60wt%, 62.8wt%, 63.4wt%, 66wt%, 67.5wt%, 69wt%, 70wt%; 55wt%-65wt% more preferably; Most preferably be 60wt%.
Described thickener is preferably the mixture of any one or at least two kinds in xanthans, gum Arabic and the Magnesiumaluminumsilicate, most preferably is xanthans.
The mass percent (wt%) of described thickener in brassinosteroid biosynthesis kind clothing of the present invention agent is 0.1wt%-0.5wt%, for example can be 0.1wt%-0.13wt%, 0.17wt%-0.24wt%, 0.32wt%-0.42wt%, 0.35wt%-0.5wt%, 0.1wt%, 0.14wt%, 0.15wt%, 0.2wt%, 0.21wt%, 0.26wt%, 0.28wt%, 0.3wt%, 0.33wt%, 0.35wt%, 0.37wt%, 0.4wt%, 0.44wt%, 0.45wt%, 0.49wt%, 0.5wt%; 0.2wt%-0.4wt% more preferably; Most preferably be 0.3wt%.
Described antifreezing agent is preferably ethylene glycol and/or propane diols, most preferably is propane diols.
The mass percent (wt%) of described antifreezing agent in brassinosteroid biosynthesis kind clothing of the present invention agent is 3wt%-7wt%, for example can be 3wt%-4.5wt%, 3.9wt%-6wt%, 4.7wt%-5.4wt%, 5.2wt%-7wt%, 3wt%, 3.1wt%, 3.5wt%, 3.92wt%, 4wt%, 4.2wt%, 4.5wt%, 4.83wt%, 5wt%, 5.37wt%, 5.5wt%, 5.8wt%, 6wt%, 6.05wt%, 6.5wt%, 7wt%; 4wt%-6wt% more preferably; Most preferably be 5wt%.
Described preservative is preferably the mixture of any one or at least two kinds in Sodium Benzoate, potassium sorbate, the sodium salicylate, most preferably is potassium sorbate.
The mass percent (wt%) of described preservative in brassinosteroid biosynthesis kind clothing of the present invention agent is 0.3wt%-0.7wt%, for example can be 0.3wt%-0.5wt%, 0.32wt%-0.43wt%, 0.4wt%-0.6wt%, 0.62wt%-0.7wt%, 0.3wt%, 0.33wt%, 0.35wt%, 0.37wt%, 0.4wt%, 0.42wt%, 0.45wt%, 0.48wt%, 0.5wt%, 0.53wt%, 0.55wt%, 0.58wt%, 0.6wt%, 0.65wt%, 0.7wt%; 0.4wt%-0.6wt% more preferably; Most preferably be 0.5wt%.
Described colouring agent is preferably sarranine and/or basic rhodamine, most preferably is basic rhodamine.
The mass percent (wt%) of described colouring agent in brassinosteroid biosynthesis kind clothing of the present invention agent is 0.5wt%-1.5wt%, for example can be 0.5wt%-0.75wt%, 0.62wt%-0.8wt%, 0.7wt%-1.3wt%, 0.92wt%-1.5wt%, 0.5wt%, 0.55wt%, 0.6wt%, 0.63wt%, 0.67wt%, 0.7wt%, 0.75wt%, 0.8wt%, 0.82wt%, 0.9wt%, 0.95wt%, 1wt%, 1.16wt%, 1.25wt%, 1.3wt%, 1.39wt%, 1.4wt%, 1.46wt%, 1.5wt%; 0.8wt%-1.2wt% more preferably; Most preferably be 1wt%.
In sum, the technical scheme after the optimization of the present invention is:
The agent of a kind of brassinosteroid biosynthesis kind clothing comprises following component according to mass percent: zymotic fluid 10wt%-40wt%, the little fertile 2wt%-5wt% of active component bacillus pumilus (Bacillus pumilus) GBSC66 CGMCC No.3379, film forming agent solution 50wt%-70wt%, thickener 0.1wt%-0.5wt%, antifreezing agent 3wt%-7wt%, preservative 0.3wt%-0.7wt%, colouring agent 0.5wt%-1.5wt%, all the other are water; The concentration of bacillus pumilus in the described zymotic fluid (Bacillus pumilus) GBSC66 CGMCC No.3379 is 〉=10
7Cfu/ml.
Technical scheme after the present invention further optimizes is:
The agent of a kind of brassinosteroid biosynthesis kind clothing comprises following component according to mass percent: zymotic fluid 20wt%-30wt%, the little fertile 3wt%-4wt% of active component bacillus pumilus (Bacillus pumilus) GBSC66 CGMCC No.3379, film forming agent solution 55wt%-65wt%, thickener 0.2wt%-0.4wt%, antifreezing agent 4wt%-6wt%, preservative 0.4wt%-0.6wt%, colouring agent 0.8wt%-1.2wt%, all the other are water; The concentration of bacillus pumilus in the described zymotic fluid (Bacillus pumilus) GBSC66 CGMCC No.3379 is 10
7-10
9Cfu/ml.
The optimized technical scheme of the present invention is:
The agent of a kind of brassinosteroid biosynthesis kind clothing comprises following component according to mass percent: zymotic fluid 25wt%, the little fertile 3.5wt% of active component bacillus pumilus (Bacillus pumilus) GBSC66CGMCC No.3379, film forming agent solution 60wt%, thickener 0.3wt%, antifreezing agent 5wt%, preservative 0.5wt%, colouring agent 1wt%, all the other are water; The concentration of bacillus pumilus in the described zymotic fluid (Bacillus pumilus) GBSC66 CGMCC No.3379 is 10
8Cfu/ml.
" comprising " of the present invention, mean it except described component, can also contain other components, these other components are given the agent of described brassinosteroid biosynthesis kind clothing with different characteristics.In addition, " comprising " of the present invention, can also replace with enclosed " being " or " by ... make ".No matter which kind of composition brassinosteroid biosynthesis kind clothing of the present invention agent comprises, the percentage by weight sum of described brassinosteroid biosynthesis kind clothing agent is 100%.
For achieve the above object two, the present invention adopts following technical scheme:
The preparation method of a kind of brassinosteroid biosynthesis kind clothing agent comprises the steps:
(1) bacillus pumilus (Bacillus pumilus) GBSC66 CGMCC No.3379 is fermented, it is for subsequent use to obtain the satisfactory zymotic fluid of concentration;
(2) zymotic fluid of step (1) gained and other component in the prescription are mixed according to the ratio in the prescription, namely get brassinosteroid biosynthesis kind clothing of the present invention agent.
For achieve the above object three, the present invention adopts following technical scheme:
The using method of a kind of brassinosteroid biosynthesis kind clothing agent is with the agent of described brassinosteroid biosynthesis kind clothing rape seed to be carried out dressing.
Preferably, the brassinosteroid biosynthesis kind clothing agent that described dressing uses and the weight ratio of rape seed are 1:50-1:300, for example can be 1:50-1:90,1:75-1:110,1:144-1:205,1:190-1:280,1:225-1:300,1:50,1:60,1:88,1:100,1:127,1:150,1:168,1:195,1:203,1:230,1:250,1:264,1:275,1:300; 1:100-1:200 more preferably; Most preferably be 1:150.
For achieve the above object four, the present invention adopts following technical scheme:
The application of a kind of brassinosteroid biosynthesis kind clothing agent in the control sclerotinia rot of colza.
The application of a kind of brassinosteroid biosynthesis kind clothing agent in promoting growth of rape.
Brassinosteroid biosynthesis kind clothing provided by the invention agent on rape seed, has drug effect height, safety, noresidue, free of contamination advantage with the technology first Application of biological seed coating agent, and is to seed safety, nuisanceless to environment; Particularly physical property is good, and imbibition is good and without coming off, and can bring into play lastingly drug effect.After with brassinosteroid biosynthesis kind clothing of the present invention agent rape seed being carried out dressing, can strengthen seed vitality, improve resistance, promote plant strain growth, improve output, not only can significantly promote growing of rape, can also effectively prevent and treat sclerotinia rot of colza.Simultaneously, brassinosteroid biosynthesis kind clothing agent and process for producing same of the present invention is simple, with low cost, use conveniently, the storage life is long, is suitable for large-scale industrial production.
Below in conjunction with embodiment the present invention is described in further detail.But following embodiment only is simple and easy example of the present invention, does not represent or limit the scope of the present invention, and interest field of the present invention is as the criterion with claims.
Embodiment
For the present invention is described better, be convenient to understand technical scheme of the present invention, typical but non-limiting embodiment of the present invention is as follows:
Embodiment 1:
Be prepared as follows the agent of brassinosteroid biosynthesis kind clothing:
(1) bacillus pumilus (Bacillus pumilus) GBSC66 CGMCC No.3379 is fermented, obtaining concentration is 10
8The zymotic fluid of cfu/ml is for subsequent use;
(2) get the raw materials ready according to following prescription: zymotic fluid 30wt%, manganese sulphate 2wt%, potassium chloride 1wt%, the mass concentration of bacillus pumilus (Bacillus pumilus) GBSC66 CGMCCNo.3379 is aqueous solution 50wt%, the xanthans 0.2wt% of the sodium carboxymethylcellulose of 3wt%, propane diols 5wt%, potassium sorbate 0.3wt%, basic rhodamine 0.5wt%, all the other are water;
(3) zymotic fluid of step (1) gained and other component in step (2) prescription are mixed, namely get the agent of brassinosteroid biosynthesis kind clothing.
During use, the kind clothing agent of present embodiment preparation is carried out dressing with the weight ratio of 1:150 to rape seed.
Embodiment 2:
Be prepared as follows the agent of brassinosteroid biosynthesis kind clothing:
(1) bacillus pumilus (Bacilluspumilus) GBSC66 CGMCC No.3379 is fermented, the zymotic fluid that obtains concentration and be 107cfu/ml is for subsequent use;
(2) get the raw materials ready according to following prescription: chitosan aqueous solution 30wt%, the gum Arabic 0.4wt% that zymotic fluid 25wt%, manganese carbonate 2wt%, potassium sulphate 3wt%, mass concentration are the polyvinyl alcohol water solution 30wt% of 4wt%, mass concentration is 2wt% of bacillus pumilus (Bacillus pumilus) GBSC66 CGMCCNo.3379, ethylene glycol 3wt%, Sodium Benzoate 0.4wt%, sarranine 0.8wt%, all the other are water;
(3) zymotic fluid of step (1) gained and other component in step (2) prescription are mixed, namely get the agent of brassinosteroid biosynthesis kind clothing.
During use, the kind clothing agent of present embodiment preparation is carried out dressing with the weight ratio of 1:50 to rape seed.
Embodiment 3:
Be prepared as follows the agent of brassinosteroid biosynthesis kind clothing:
(1) bacillus pumilus (Bacillus pumilus) GBSC66 CGMCC No.3379 is fermented, the zymotic fluid that obtains concentration and be 109cfu/ml is for subsequent use;
(2) get the raw materials ready according to following prescription: zymotic fluid 40wt%, the potassium dihydrogen phosphate 3.5wt% of bacillus pumilus (Bacillus pumilus) GBSC66 CGMCCNo.3379, chitosan aqueous solution 55wt%, the Magnesiumaluminumsilicate 0.1wt% that mass concentration is 2wt%, ethylene glycol 2wt%, propane diols 2wt%, potassium sorbate 0.2wt%, sodium salicylate 0.3wt%, sarranine 0.5wt%, basic rhodamine 0.5wt%, all the other are water;
(3) zymotic fluid of step (1) gained and other component in step (2) prescription are mixed, namely get the agent of brassinosteroid biosynthesis kind clothing.
During use, the kind clothing agent of present embodiment preparation is carried out dressing with the weight ratio of 1:300 to rape seed.
Embodiment 4:
Be prepared as follows the agent of brassinosteroid biosynthesis kind clothing:
(1) bacillus pumilus (Bacillus pumilus) GBSC66 CGMCC No.3379 is fermented, obtaining concentration is 10
10The zymotic fluid of cfu/ml is for subsequent use;
(2) get the raw materials ready according to following prescription: zymotic fluid 20wt%, the manganese chloride 2wt% of bacillus pumilus (Bacillus pumilus) GBSC66 CGMCCNo.3379, polyvinyl alcohol water solution 65wt%, the Magnesiumaluminumsilicate 0.3wt% that mass concentration is 4wt%, gum Arabic 0.2wt%, ethylene glycol 6wt%, Sodium Benzoate 0.2wt%, sodium salicylate 0.2wt%, potassium sorbate 0.3wt%, sarranine 1.2wt%, all the other are water;
(3) zymotic fluid of step (1) gained and other component in step (2) prescription are mixed, namely get the agent of brassinosteroid biosynthesis kind clothing.
During use, the kind clothing agent of present embodiment preparation is carried out dressing with the weight ratio of 1:200 to rape seed.
Embodiment 5:
Be prepared as follows the agent of brassinosteroid biosynthesis kind clothing:
(1) bacillus pumilus (Bacillus pumilus) GBSC66 CGMCC No.3379 is fermented, obtaining concentration is 10
8The zymotic fluid of cfu/ml is for subsequent use;
(2) get the raw materials ready according to following prescription: aqueous solution 70wt%, the xanthans 0.3wt% of zymotic fluid 10wt%, the potassium chloride 4wt% of bacillus pumilus (Bacillus pumilus) GBSC66 CGMCCNo.3379, the sodium carboxymethylcellulose that mass concentration is 3wt%, propane diols 7wt%, potassium sorbate 0.6wt%, basic rhodamine 1.5wt%, all the other are water;
(3) zymotic fluid of step (1) gained and other component in step (2) prescription are mixed, namely get the agent of brassinosteroid biosynthesis kind clothing.
During use, the kind clothing agent of present embodiment preparation is carried out dressing with the weight ratio of 1:100 to rape seed.
Test example:
For investigating brassinosteroid biosynthesis kind clothing of the present invention agent in actual applications for come into force fruit and to the control efficiency of sclerotinia rot of colza of rape short, it is as follows to carry out the greenhouse pot culture test:
With the weight ratio of 1:150 rape seed is carried out dressing with the brassinosteroid biosynthesis kind clothing agent that embodiment 1 obtains, as experimental group;
Under the concentration of pesticide-seeds ratio 1:60, rape seed is carried out dressing with chemical seed clothing agent good fortune more than 10% suspension seed-coating agent (the Shanghai general digests Co., Ltd to be provided), as the first control group;
With the processing rape seed of clear water as the second control group.
Sow in the usual way after rape seed after above-mentioned three groups processed dries and filling sterilization soil and be mixed with in the flowerpot of sclerotinite, after 40 days, observe the incidence of respectively organizing rape and the situation of growing, the result is as shown in table 1 below:
Table 1
As can be seen from Table 1, brassinosteroid biosynthesis kind clothing of the present invention agent does not almost affect for the germination rate of rape, only the germination rate of a little higher than two control groups.And on the growth indexes such as plant height, fresh weight, root length, all obviously be better than two control groups, illustrate that brassinosteroid biosynthesis kind clothing of the present invention agent can effectively promote growth of rape.Relatively the disease index of each processed group can find out that the rape incidence of experimental group is the lightest, reaches 61.68% for the control efficiency of sclerotinia rot of colza, obviously is better than the first control group that chemical pesticide is processed.
Should be noted that and understand, in the situation that does not break away from the desired the spirit and scope of the present invention of accompanying claim, can make to the present invention of foregoing detailed description various modifications and improvement.Therefore, the scope of claimed technical scheme is not subjected to the restriction of given any specific exemplary teachings.
Applicant's statement, above content is the further description of the present invention being done in conjunction with concrete preferred embodiment, can not assert that implementation of the present invention is confined to these explanations.For the general technical staff of the technical field of the invention, without departing from the inventive concept of the premise, can also make some simple deduction or replace, all should be considered as belonging to protection scope of the present invention.
Claims (10)
1. brassinosteroid biosynthesis kind clothing agent is characterized in that its active component is the biocontrol microorganisms zymotic fluid, and described biocontrol microorganisms is bacillus pumilus (Bacillus pumilus) GBSC66 CGMCC No.3379.
2. brassinosteroid biosynthesis kind clothing according to claim 1 agent is characterized in that the concentration of biocontrol microorganisms is 〉=10 in the described biocontrol microorganisms zymotic fluid
7Cfu/ml is preferably 10
7-10
9Cfu/ml; Most preferably be 10
8Cfu/ml.
3. brassinosteroid biosynthesis kind clothing according to claim 1 and 2 agent is characterized in that, the mass percent of described biocontrol microorganisms zymotic fluid in the agent of described brassinosteroid biosynthesis kind clothing is 10wt%-40wt%, is preferably 20wt%-30wt%, most preferably is 25wt%.
4. one of according to claim 1-3 described brassinosteroid biosynthesis kind clothing agent is characterized in that, also comprises little fertilizer, film forming agent solution, thickener, antifreezing agent, preservative, colouring agent and water.
5. one of according to claim 1-4 described brassinosteroid biosynthesis kind clothing agent is characterized in that described little fertilizer is that copper fertilizer, boron fertilizer, molydbenum fertilizer, manganese fertilizer, iron fertilizer and zinc fertilizer etc. can use in the present invention; Be preferably manganese fertilizer and potash fertilizer, such as manganese sulphate, manganese carbonate, manganese chloride, potassium chloride, potassium sulphate, potassium dihydrogen phosphate, potassium magnesium salts, potassium nitrate etc. are arranged; Most preferably be manganese sulphate and/or potassium chloride.
Preferably, described film forming agent solution is the mixture of any one or at least two kinds in the chitosan aqueous solution that mass concentration is the aqueous solution of the sodium carboxymethylcellulose of 3wt%, polyvinyl alcohol water solution that mass concentration is 4wt%, mass concentration is 2wt%, most preferably is the aqueous solution that mass concentration is the sodium carboxymethylcellulose of 3wt%;
Preferably, described thickener is the mixture of any one or at least two kinds in xanthans, gum Arabic and the Magnesiumaluminumsilicate, most preferably is xanthans;
Preferably, described antifreezing agent is ethylene glycol and/or propane diols, most preferably is propane diols.
Preferably, described preservative is the mixture of any one or at least two kinds in Sodium Benzoate, potassium sorbate, the sodium salicylate, most preferably is potassium sorbate;
Preferably, described colouring agent is sarranine and/or basic rhodamine, most preferably is basic rhodamine.
6. one of according to claim 1-5 described brassinosteroid biosynthesis kind clothing agent is characterized in that the mass percent of described little fertilizer in the agent of described brassinosteroid biosynthesis kind clothing is 2wt%-5wt%, is preferably 3wt%-4wt%, most preferably is 3.5wt%;
Preferably, the mass percent 50wt%-70wt% of described film forming agent solution in the agent of described brassinosteroid biosynthesis kind clothing, more preferably 55wt%-65wt% most preferably is 60wt%;
Preferably, the mass percent of described thickener in the agent of described brassinosteroid biosynthesis kind clothing is 0.1wt%-0.5wt%, and more preferably 0.2wt%-0.4wt% most preferably is 0.3wt%;
Preferably, the mass percent of described antifreezing agent in the agent of described brassinosteroid biosynthesis kind clothing is 3wt%-7wt%, and more preferably 4wt%-6wt% most preferably is 5wt%;
Preferably, the mass percent of described preservative in the agent of described brassinosteroid biosynthesis kind clothing is 0.3wt%-0.7wt%, and more preferably 0.4wt%-0.6wt% most preferably is 0.5wt%;
Preferably, the mass percent of described colouring agent in the agent of described brassinosteroid biosynthesis kind clothing is 0.5wt%-1.5wt%, and more preferably 0.8wt%-1.2wt% most preferably is 1wt%.
7. one of according to claim 1-6 the preparation method of described brassinosteroid biosynthesis kind clothing agent is characterized in that, comprises the steps:
(1) bacillus pumilus (Bacillus pumilus) GBSC66 CGMCC No.3379 is fermented, it is for subsequent use to obtain the satisfactory zymotic fluid of concentration;
(2) zymotic fluid of step (1) gained and other component in the prescription are mixed according to the ratio in the prescription, namely get brassinosteroid biosynthesis kind clothing of the present invention agent.
8. one of according to claim 1-6 the using method of described brassinosteroid biosynthesis kind clothing agent is characterized in that described method is with the agent of described brassinosteroid biosynthesis kind clothing rape seed to be carried out dressing;
Preferably, the brassinosteroid biosynthesis kind clothing agent that described dressing uses and the weight ratio of rape seed are 1:50-1:300, and more preferably 1:100-1:200 most preferably is 1:150.
9. one of according to claim 1-6 application of described brassinosteroid biosynthesis kind clothing agent in the control sclerotinia rot of colza.
10. one of according to claim 1-6 application of described brassinosteroid biosynthesis kind clothing agent in promoting growth of rape.
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