CN103004884B - Watermelon biological seed coating agent and Synthesis and applications thereof - Google Patents
Watermelon biological seed coating agent and Synthesis and applications thereof Download PDFInfo
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- CN103004884B CN103004884B CN201210435041.4A CN201210435041A CN103004884B CN 103004884 B CN103004884 B CN 103004884B CN 201210435041 A CN201210435041 A CN 201210435041A CN 103004884 B CN103004884 B CN 103004884B
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Abstract
The invention discloses a kind of watermelon biological seed coating agent and its preparation method and application.Described watermelon biological seed coating agent effectively can prevent and treat the multiple soil-borne disease of watermelon, and has growth-promoting functions to watermelon, can significantly improve the yield of watermelon, simultaneously nuisanceless to environment, is the biological seed coating agent of a kind of low toxicity, safety.Described watermelon biological seed coating agent producing process is simple, and production cost is lower, and use conveniently, the storage life is long, is suitable for large-scale industrial production and seed treatment.
Description
Technical field
The present invention relates to a kind of biological pesticide preparation, specifically, is a kind of watermelon biological seed coating agent and Synthesis and applications thereof.
Background technology
Watermelon is annual climing property herbaceous plant.Melon pulp is tender and crisp, the sweet succulence of taste.Except not fatty and cholesterol, watermelon contains the materials such as a large amount of glucose, malic acid, fructose, Argine Monohydrochloride, tomatin and abundant vitamin C, be main fruit of relieving summer heat in summer, and have auxiliary curative effect to diseases such as treatment ephritis, diabetes and cystitiss.In addition, its seed oil content reaches 50%, can extract oil, stir-fries and eats or do cake batching.Watermelon disease has kind more than 10, wherein endangering more serious has watermelon grafting, watermelon blight, watermelon anthrax, watermelon virus disease, watermelon powdery mildew, watermelon leaf rot, watermelon epidemic disease, watermelon root-knot sick, the harm of these diseases not only affects the yield of watermelon, also affects quality.Wherein watermelon blight, watermelon damping off, watermelon damping off etc. endanger larger disease mainly through soil-borne, by carrying out Cotton seeds to seed to carry out effectively preventing.
Kind of clothing technology is the seed processing technology that Application comparison is general in the world grown up on the basis of tradition seed soaking, seed dressing, is the combination of the standardization of seed quality, the modernization of seeds processing and field integrated pest prevention and control in seedling stage.Use kind of a clothing technology to carry out process to crop seed and there is following benefit: (1) by desinsection, sterilization, kill mouse, sterilization compound, coordinate plant growth; (2) change subenvironment around seed, nutrition and oxygen be provided, promote that plant early germinates, it is fast to grow, early ripe; (3) regulate shape of the seed, size, be beneficial to machine sowing, and break the seed dormancy phase, reach the object etc. of sowing continuously.Plant the seed coat agent adopted in clothing technology to be usually made up of active component and non-active ingredient.Active component mainly comprises insecticide, bactericide, hormone, fertilizer or beneficial microbe and metabolite etc. thereof, is the active ingredient directly played a role in seed coat agent.Non-active ingredient refers to film forming agent in seed coat agent and corresponding additive, the physics and the chemical property that maintain seed coat agent, the important component of active component slowly-releasing in controlling diaphragm, comprises film forming agent, dispersant, suspending agent, antifreezing agent, warning colouration, defoamer, thickener, preservative etc.The composition of non-active ingredient directly affects quality and the coating effect of seed coat agent.What current use was more is chemical seed coat agent, and it has high malicious high residue and the series of problems such as the active component lasting period is short.
Bio-seed Coating agent is a kind of novel seed coat agent, it is the important component part of biopesticide, its active component to control the natural products of phytopathogen, living microorganisms or its metabolite, mainly comprise microbiocidal (living microorganism and farm antibiotics etc.), plant source type, take microbial metabolic products as the formulation, genetic engineering bacterial strain etc. of guide's synthesis, effectively can overcome the height poison high residue of chemical seed coat agent and the problem such as the active component lasting period is short, to crop itself, also there is certain trophic function, the quality of crop can be improved.But Bio-seed Coating agent, the specific aim especially with the Bio-seed Coating agent of prevention and elimination of disease and pests function is relatively strong, and different crops, according to the difference of its processing intent, needs the Bio-seed Coating agent of difference in functionality.
Bacillus (Bacillus spp.) is important plant growth-promoting rhizobacteria (Plant Growth-Promoting Rhizobacteria, PGPR), and addicted to warm, aerobic, product gemma, its physiological characteristic is various, widely distributed, is very easily separated cultivation.This bacterium is widespread in nature, nontoxic to people and animals, free from environmental pollution, can produce multiple antibiotic and enzyme, has broad spectrum antibiotic activity and extremely strong anti-adversity ability.There is due to bacillus the prominent features such as growth is fast, nutrition is simple and it is heat-resisting to produce, degeneration-resistant gemma, and make its be not only conducive to biocontrol fungicide in production, formulation and survival in the environment, surely to grow and breeding, and mass production processes is simple, cost is lower, use conveniently, storage life is long, has now become a kind of desirable biocontrol bacteria.
In the art, having for comprising the multiple soil-borne disease effectively can preventing and treating watermelon, improving the needs of the new bio type seed coat agent of the yield of watermelon.
Summary of the invention
The object of the invention is to provide a kind of, low toxicity, safe biological seed coating agent nuisanceless to environment, and it not only has growth-promoting functions to watermelon, and effectively can prevent and treat the multiple soil-borne disease of watermelon, improves the yield of watermelon.
The present invention is realized by following technological means:
In one aspect of the invention, provide a kind of biological seed coating agent, described biological seed coating agent comprises biocontrol microorganisms zymotic fluid, described biocontrol microorganisms is that bacillus pumilus (Bacillus pumilus) LNXM12(is preserved in (No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), Institute of Microorganism, Academia Sinica), preservation date is on November 02nd, 2009, and preserving number is CGMCC No.3380).
In biological seed coating agent of the present invention, in described biocontrol microorganisms zymotic fluid, cell concentration can 10
8more than cfu/mL.
Biological seed coating agent of the present invention can also comprise plant growth regulator, nutritive element and film forming agent solution, and optionally dispersant, thickener, antifreezing agent, preservative, colouring agent or its combination of at least 2 kinds.
Biological seed coating agent of the present invention can be mixed by following composition according to percentage by weight: biocontrol microorganisms zymotic fluid 10% ~ 30%, preferably 15-25%, and more preferably 20%; Plant growth regulator 0.01 ~ 0.05%, preferably 0.02-0.04%, more preferably 0.03%; Nutritive element 10 ~ 15%, preferably 11-14%, more preferably 12-13%; Film forming agent solution 40% ~ 60%, preferably 45-55%, more preferably 50%; Dispersant 1% ~ 3%, preferably 1.5-2.5%, more preferably 2%; Thickener 0.3% ~ 0.7%, preferably 0.4-0.6%, more preferably 0.5%; Antifreezing agent 3% ~ 8%, preferably 4-7%, more preferably 5-6%; Preservative 0.3% ~ 1.0%, preferably 0.5-0.9%, more preferably 0.7%; Colouring agent 0.5% ~ 1.5%, preferably 0.7-1.3%, more preferably 1.0%; Be water with surplus.
In biological seed coating agent of the present invention, the percentage by weight of biocontrol microorganisms zymotic fluid can be 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29% or 30%.
In biological seed coating agent of the present invention, the percentage by weight of plant growth regulator can be 0.01%, 0.02%, 0.03%, 0.04% or 0.05%.
In biological seed coating agent of the present invention, the percentage by weight of nutritive element can be 10%, 11%, 12%, 13%, 14% or 15%.
In biological seed coating agent of the present invention, the percentage by weight of film forming agent solution can be 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59% or 60%.
In biological seed coating agent of the present invention, the percentage by weight of dispersant can be 1%, 1.5%, 2%, 2.5% or 3%.
In biological seed coating agent of the present invention, the percentage by weight of thickener can be 0.3%, 0.4%, 0.5%, 0.6% or 0.7%.
In biological seed coating agent of the present invention, the percentage by weight of antifreezing agent can be 3%, 4%, 5%, 6%, 7% or 8%.
In biological seed coating agent of the present invention, the percentage by weight of preservative can be 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9% or 1.0%.
In biological seed coating agent of the present invention, the percentage by weight of colouring agent can be 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, 1.2%, 1.3%, 1.4% or 1.5%.
In biological seed coating agent of the present invention, in described biocontrol microorganisms zymotic fluid, cell concentration can 10
8more than cfu/mL.
In biological seed coating agent of the present invention, described plant growth regulator can be methyl α-naphthyl acetate;
In biological seed coating agent of the present invention, described nutritive element can be made up of the following material of percentage by weight: potassium sulfate 1-4%, preferably 2-3%, and more preferably 2.5%; Boric acid 0.5-0.7%, preferably 0.55-0.65%, more preferably 0.6%; Manganese sulphate 0.2-0.4%, preferably 0.25-0.35%, more preferably 0.3%; Zinc sulphate 0.1-0.5%, preferably 0.2-0.4%, more preferably 0.3%; With copper sulphate 0.1-0.2%, preferably 0.12-0.18%, more preferably 0.15%.
Wherein, the percentage by weight that potassium sulfate is passable is 1%, 1.5%, 2%, 2.5%, 3%, 3.5% or 4%; The percentage by weight of boric acid can be 0.5%, 0.55%, 0.6%, 0.65% or 0.7%; The percentage by weight of manganese sulphate can be 0.2%, 0.25%, 0.3%, 0.35% or 0.4%; The percentage by weight of zinc sulphate can be 0.1%, 0.2%, 0.3%, 0.4% or 0.5%; The percentage by weight of copper sulphate can be 0.1%, 0.11%, 0.12%, 0.13%, 0.14%, 0.15%, 0.16%, 0.17%, 0.18%, 0.19% or 0.2%.
In biological seed coating agent of the present invention, described film forming agent solution can be the mixture of any one or at least two kinds in 3% sodium carboxymethylcellulose and 4% polyvinyl alcohol and 2% shitosan;
In biological seed coating agent of the present invention, described dispersant can be sodium lignin sulfonate, the mixture of any one or at least two kinds in neopelex and calcium lignosulfonate;
In biological seed coating agent of the present invention, described thickener can be the mixture of any one or at least two kinds in xanthans, gum Arabic and Magnesiumaluminumsilicate;
In biological seed coating agent of the present invention, described antifreezing agent can be ethylene glycol and/or propane diols;
In biological seed coating agent of the present invention, described preservative can be the mixture of any one or at least two kinds in Sodium Benzoate, potassium sorbate and sodium salicylate;
In biological seed coating agent of the present invention, described colouring agent can be sarranine or basic rhodamine.
In a second aspect of the present invention, provide the preparation method of biological seed coating agent as described in relation to the first aspect, comprise the steps:
(1) by bacillus pumilus LNXM12 by fermentation, bacteria concentration>=10 are obtained
8the zymotic fluid of cfu/ml, for subsequent use;
(2) film forming agent is dissolved in the hot water, obtained film forming agent solution.
(3) above-mentioned film forming agent solution is mixed in certain proportion with the zymotic fluid of bacillus pumilus LNXM12, and stir;
(4) in above-mentioned mixed liquor, add a certain proportion of plant growth regulator, nutritive element, dispersant, thickener, antifreezing agent, preservative, colouring agent respectively, stir, obtain described biological seed coating agent.
In a third aspect of the present invention, provide the using method of biological seed coating agent as described in relation to the first aspect, it comprises and being contacted with watermelon seed by described biological seed coating agent.
In the using method of described biological seed coating agent of the present invention, described biological seed coating agent and watermelon seed the weight ratio of 1:50-1:200 can carry out dressing, preferably carry out dressing with the weight ratio of 1:70-1:150, more preferably carry out dressing with the weight ratio of 1:100.
In a fourth aspect of the present invention, provide the purposes of biological seed coating agent in the control of watermelon soil-borne disease as described in relation to the first aspect.
In the purposes of biological seed coating agent as described in relation to the first aspect of the present invention, described watermelon soil-borne disease is watermelon blight, watermelon damping off and/or watermelon damping off.
In the present invention, soil-borne disease refers to that pathogene such as fungi, bacterium, nematode and virus are lived in soil with invalid body, the disease caused from crop root or stem infringement crop when condition is suitable for.
Beneficial effect of the present invention is as follows:
(1) biological seed coating agent of the present invention effectively can prevent and treat the multiple soil-borne disease of watermelon, and has growth-promoting functions to watermelon, can significantly improve the yield of watermelon;
(2) biological seed coating agent of the present invention is nuisanceless to environment, is the biological seed coating agent of a kind of low toxicity, safety;
(3) biological seed coating agent of the present invention is used conveniently, and the storage life is long, is suitable for large-scale seed treatment;
(4) biological seed coating agent producing process of the present invention is simple, and production cost is lower, is suitable for large-scale industrial production.
Embodiment
The present invention is further described below by way of embodiment, as known by the technical knowledge, the present invention also describes by other the scheme not departing from the technology of the present invention feature, and the change therefore within the scope of the present invention all or equivalent scope of the invention is all included in the invention.
Embodiment 1:
The present embodiment seed coat agent is formulated with following weight ratio by each composition:
Biocontrol microorganisms zymotic fluid 30%, plant growth regulator 0.02%, nutritive element 10%, film forming agent solution 50%(3% carboxymethylcellulose sodium solution), thickener 0.3%(xanthans), dispersant 2.5%(sodium lignin sulfonate), antifreezing agent 5%(ethylene glycol), preservative 0.5%(Sodium Benzoate), colouring agent 0.5%(sarranine), all the other are water.
Wherein the percentage by weight of nutritive element consists of potassium sulfate 4%, boric acid 0.5%, manganese sulphate 0.4%, zinc sulphate 0.1%, copper sulphate 0.2%, and all the other are water.
Embodiment 2:
The present embodiment seed coat agent is formulated with following weight ratio by each composition:
Biocontrol microorganisms zymotic fluid 20%, plant growth regulator 0.05%, nutritive element 15%, film forming agent solution 40%(4% poly-vinyl alcohol solution), thickener 0.7%(gum Arabic), dispersant 2.5%(sodium lignin sulfonate), antifreezing agent 5%(ethylene glycol), preservative 0.3%(potassium sorbate), colouring agent 0.5%(sarranine), all the other are water.
Wherein the percentage by weight of nutritive element consists of potassium sulfate 1%, boric acid 0.7%, manganese sulphate 0.2%, zinc sulphate 0.5%, copper sulphate 0.1%, and all the other are water.
Embodiment 3:
The present embodiment seed coat agent is formulated with following weight ratio by each composition:
Biocontrol microorganisms zymotic fluid 30%, plant growth regulator 0.03%, nutritive element 12%, film forming agent solution 45%(2% chitosan solution), thickener 0.5%(aluminium-magnesium silicate), dispersant 3%(neopelex), antifreezing agent 3%(propane diols), preservative 0.7%(sodium salicylate), colouring agent 1%(basic rhodamine), all the other are water.
Wherein the percentage by weight of nutritive element consists of potassium sulfate 2%, boric acid 0.6%, manganese sulphate 0.3%, zinc sulphate 0.3%, copper sulphate 0.15%, and all the other are water.
Embodiment 4
The present embodiment seed coat agent is formulated with following weight ratio by each composition:
Biocontrol microorganisms zymotic fluid 10%, plant growth regulator 0.04%, nutritive element 11%, film forming agent solution 60%(3% carboxymethylcellulose sodium solution), thickener 0.4%(xanthans), dispersant 1%(calcium lignosulfonate), antifreezing agent 4%(ethylene glycol), preservative 0.9%(Sodium Benzoate), colouring agent 1.5%(sarranine), all the other are water.
Wherein the percentage by weight of nutritive element consists of potassium sulfate 3%, boric acid 0.55%, manganese sulphate 0.35%, zinc sulphate 0.2%, copper sulphate 0.19%, and all the other are water.
Embodiment 5
The present embodiment seed coat agent is formulated with following weight ratio by each composition:
Biocontrol microorganisms zymotic fluid 25%, plant growth regulator 0.01%, nutritive element 13%, film forming agent solution 45%(2% shitosan), thickener 0.6%(Magnesiumaluminumsilicate), dispersant 2%(neopelex), antifreezing agent 8%(ethylene glycol), preservative 1%(sodium salicylate), colouring agent 1.2%(sarranine), surplus is water
Wherein the percentage by weight of nutritive element consists of potassium sulfate 2%, boric acid 0.65%, manganese sulphate 0.25%, zinc sulphate 0.4%, copper sulphate 0.13%, and all the other are water.
Embodiment 6
The present embodiment seed coat agent is formulated with following weight ratio by each composition:
Biocontrol microorganisms zymotic fluid 15%, plant growth regulator 0.03%, nutritive element 14%, film forming agent solution 55%(2% shitosan), thickener 0.4%(Magnesiumaluminumsilicate), dispersant 1.5%(sodium lignin sulfonate), antifreezing agent 7%(ethylene glycol), preservative 0.6%(sodium salicylate), colouring agent 0.8%(sarranine), surplus is water
Wherein the percentage by weight of nutritive element consists of potassium sulfate 3%, boric acid 0.6%, manganese sulphate 0.3%, zinc sulphate 0.3%, copper sulphate 0.17%, and all the other are water.
Preparation method is identical for above-described embodiment seed coat agent, first by bacillus pumilus LNXM12 by fermentation, obtains bacteria concentration>=10
8the zymotic fluid of cfu/ml, for subsequent use; Film forming agent is dissolved in the hot water, obtained film forming agent solution; Above-mentioned film forming agent solution is mixed with corresponding proportion with the zymotic fluid of bacillus pumilus LNXM12, and stirs; In above-mentioned mixed liquor, add plant growth regulator, nutritive element, dispersant, thickener, antifreezing agent, preservative, the colouring agent of corresponding proportion respectively, stir, obtain invented biological seed coating agent.
Obtained seed coat agent and watermelon seed carry out dressing with the weight ratio of 1:50-1:200, effectively can prevent and treat the multiple soil-borne disease of watermelon.
In order to investigate developed biological seed coating agent action effect in actual applications, do pot experiment as follows.
Test example 1:
This test example is the research diseases prevention of biological seed coating agent to watermelon blight and the growth-promoting effect to watermelon, has done following test:
With the weight ratio of 1:200, dressing is carried out to watermelon seed by the seed coat agent of preparation of filling a prescription described in embodiment 1, establish two groups of contrasts, the U.S. Seedvax suspension seed-coating agent of first chemical pesticide 10% good fortune (being produced by Jiangsu Hua Nong seed coat agent Co., Ltd) carries out dressing to watermelon seed with the weight ratio of 1:50 simultaneously; It two is with clear water process watermelon seed.Sow in the usual way after capsuled seed is dried and filling sterilized soil and be mixed with in the flowerpot of watermelon blight pathogen (Fusarium oxysporum watermelon transformant).Grow after one month, the incidence of watermelon after checking different modes process, and situation of growing, result is as shown in table 1:
The growth of watermelon and incidence after the process of table 1. different modes
As shown in Table 1, by the watermelon of biological seed coating agent dressing of the present invention no matter at germination rate or at plant height, fresh weight, the growth indexes such as root is long is all better than the watermelon of chemical pesticide and clear water process, illustrates that biological seed coating agent of the present invention effectively can promote watermelon growing.The disease index of relatively more each process is known, falls ill the lightest, reach 76.74%, be better than chemical pesticide process to the preventive effect of watermelon blight by the watermelon of biological seed coating agent process.Illustrate that biological seed coating agent of the present invention has good control efficiency to watermelon blight.
Test example 2:
This test example is the research diseases prevention of biological seed coating agent to watermelon damping off and the growth-promoting effect to watermelon, has done following test:
With the weight ratio of 1:150, dressing is carried out to watermelon seed by the seed coat agent of preparation of filling a prescription described in embodiment 3, establish two groups of contrasts, the U.S. Seedvax suspension seed-coating agent of first chemical pesticide 10% good fortune (being produced by Jiangsu Hua Nong seed coat agent Co., Ltd) carries out dressing to watermelon seed with the weight ratio of 1:50 simultaneously; It two is with clear water process watermelon seed.Sow in the usual way after capsuled seed is dried and filling sterilized soil and be mixed with in the flowerpot of watermelon damping off pathogen (Rhizoctonia solani Kuhn).Grow after one month, the incidence of watermelon after checking different modes process, and situation of growing, result is as shown in table 2:
The growth of watermelon and incidence after the process of table 2. different modes
As shown in Table 2, by the watermelon of biological seed coating agent dressing of the present invention no matter at germination rate or at plant height, fresh weight, the growth indexes such as root is long is all better than the watermelon of chemical pesticide and clear water process, illustrates that biological seed coating agent of the present invention effectively can promote watermelon growing.The disease index of relatively more each process is known, falls ill the lightest, reach 78.20%, be better than the chemical pesticide process that preventive effect is 35.54% to the preventive effect of watermelon damping off by the watermelon of biological seed coating agent process.Illustrate that biological seed coating agent of the present invention has good control efficiency to watermelon damping off.
Test example 3
This test example is the research diseases prevention of biological seed coating agent to watermelon damping off and the growth-promoting effect to watermelon, has done following test:
With the weight ratio of 1:100, dressing is carried out to watermelon seed by the seed coat agent of preparation of filling a prescription described in embodiment 2, establish two groups of contrasts, the U.S. Seedvax suspension seed-coating agent of first chemical pesticide 10% good fortune (being produced by Jiangsu Hua Nong seed coat agent Co., Ltd) carries out dressing to watermelon seed with the weight ratio of 1:50 simultaneously; It two is with clear water process watermelon seed.Sow in the usual way after capsuled seed is dried and filling sterilized soil and be mixed with in the flowerpot of watermelon damping off pathogen (scraping and printing unit).Grow after one month, the incidence of watermelon after checking different modes process, and situation of growing, result is as shown in table 3:
The growth of watermelon and incidence after the process of table 3. different modes
As shown in Table 3, by the watermelon of biological seed coating agent dressing of the present invention no matter at germination rate or at plant height, fresh weight, the growth indexes such as root is long is all better than the watermelon of chemical pesticide and clear water process, illustrates that biological seed coating agent of the present invention effectively can promote watermelon growing.The disease index of relatively more each process is known, falls ill the lightest, reach 77.32%, be better than the chemical pesticide process that preventive effect is 35.75% to the preventive effect of watermelon damping off by the watermelon of biological seed coating agent process.Illustrate that biological seed coating agent of the present invention has good control efficiency to watermelon damping off.
Applicant states, the present invention illustrates detailed features of the present invention and method by above-described embodiment, but the present invention is not limited to above-mentioned detailed features and method, does not namely mean that the present invention must rely on above-mentioned detailed structure feature and method could be implemented.Person of ordinary skill in the field should understand, any improvement in the present invention, to equivalence replacement and the increase of helper component, the concrete way choice etc. of component selected by the present invention, all drops within protection scope of the present invention and open scope.
Claims (38)
1. for preventing and treating a biological seed coating agent for watermelon soil-borne disease, it is characterized in that, described biological seed coating agent is become to be grouped into by following according to percentage by weight:
Biocontrol microorganisms zymotic fluid 10% ~ 30%, plant growth regulator 0.01 ~ 0.05%, nutritive element 10 ~ 15%, film forming agent solution 40% ~ 55%, dispersant 1% ~ 3%, thickener 0.3% ~ 0.7%, antifreezing agent 3% ~ 8%, preservative 0.3% ~ 1.0%, colouring agent 0.5% ~ 1.5%, surplus is water;
Biocontrol microorganisms in described biocontrol microorganisms zymotic fluid is bacillus pumilus (Bacillus pumilus) LNXM12, and preserving number is CGMCC No.3380, and its concentration in biocontrol microorganisms zymotic fluid is 10
8more than cfu/mL.
2. biological seed coating agent as claimed in claim 1, it is characterized in that, the percentage by weight of described biocontrol microorganisms zymotic fluid in biological seed coating agent is 15-25%.
3. biological seed coating agent as claimed in claim 2, it is characterized in that, the percentage by weight of described biocontrol microorganisms zymotic fluid in biological seed coating agent is 20%.
4. biological seed coating agent as claimed in claim 1, it is characterized in that, the percentage by weight of described plant growth regulator in biological seed coating agent is 0.02-0.04%.
5. biological seed coating agent as claimed in claim 4, it is characterized in that, the percentage by weight of described plant growth regulator in biological seed coating agent is 0.03%.
6. biological seed coating agent as claimed in claim 1, it is characterized in that, the percentage by weight of described nutritive element in biological seed coating agent is 11-14%.
7. biological seed coating agent as claimed in claim 6, it is characterized in that, the percentage by weight of described nutritive element in biological seed coating agent is 12-13%.
8. biological seed coating agent as claimed in claim 1, it is characterized in that, the percentage by weight of described film forming agent solution in biological seed coating agent is 45-55%.
9. biological seed coating agent as claimed in claim 8, it is characterized in that, the percentage by weight of described film forming agent solution in biological seed coating agent is 50%.
10. biological seed coating agent as claimed in claim 1, it is characterized in that, the percentage by weight of described dispersant in biological seed coating agent is 1.5-2.5%.
11. biological seed coating agents as claimed in claim 10, it is characterized in that, the percentage by weight of described dispersant in biological seed coating agent is 2%.
12. biological seed coating agents as claimed in claim 1, it is characterized in that, the percentage by weight of described thickener in biological seed coating agent is 0.4-0.6%.
13. biological seed coating agents as claimed in claim 12, it is characterized in that, the percentage by weight of described thickener in biological seed coating agent is 0.5%.
14. biological seed coating agents as claimed in claim 1, it is characterized in that, the percentage by weight of described antifreezing agent in biological seed coating agent is 4-7%.
15. biological seed coating agents as claimed in claim 14, it is characterized in that, the percentage by weight of described antifreezing agent in biological seed coating agent is 5-6%.
16. biological seed coating agents as claimed in claim 1, it is characterized in that, the percentage by weight of described preservative in biological seed coating agent is 0.5-0.9%.
17. biological seed coating agents as claimed in claim 16, it is characterized in that, the percentage by weight of described preservative in biological seed coating agent is 0.7%.
18. biological seed coating agents as claimed in claim 1, it is characterized in that, the percentage by weight of described colouring agent in biological seed coating agent is 0.7-1.3%.
19. biological seed coating agents as claimed in claim 18, it is characterized in that, the percentage by weight of described colouring agent in biological seed coating agent is 1.0%.
20. biological seed coating agents according to any one of claim 1-19, it is characterized in that, described plant growth regulator is methyl α-naphthyl acetate; Described film forming agent solution is 3% carboxymethylcellulose sodium solution and 4% poly-vinyl alcohol solution, the mixture of any one or at least two kinds in 2% chitosan solution; Described dispersant is sodium lignin sulfonate, the mixture of any one or at least two kinds in neopelex and calcium lignosulfonate; Described thickener is the mixture of any one or at least two kinds in xanthans, gum Arabic and Magnesiumaluminumsilicate; Described antifreezing agent is ethylene glycol and/or propane diols; Described preservative is the mixture of any one or at least two kinds in Sodium Benzoate, potassium sorbate and sodium salicylate; Described colouring agent is sarranine or basic rhodamine.
21. biological seed coating agents according to any one of claim 1-19, it is characterized in that, main composition and its percentage by weight of described nutritive element are:
Potassium sulfate 1-4%, boric acid 0.5-0.7%, manganese sulphate 0.2-0.4%, zinc sulphate 0.1-0.5%, copper sulphate 0.1-0.2%, all the other are water.
22. biological seed coating agents as claimed in claim 21, is characterized in that, in described nutritive element, the weight percent hundred of potassium sulfate is than being 2-3%.
23. biological seed coating agents as claimed in claim 22, is characterized in that, in described nutritive element, the weight percent hundred of potassium sulfate is than being 2.5%.
24. biological seed coating agents as claimed in claim 21, is characterized in that, in described nutritive element, the weight percent hundred of boric acid is than being 0.55-0.65%.
25. biological seed coating agents as claimed in claim 24, is characterized in that, in described nutritive element, the weight percent hundred of boric acid is than being 0.6%.
26. biological seed coating agents as claimed in claim 21, is characterized in that, in described nutritive element, the weight percent hundred of manganese sulphate is than being 0.25-0.35%.
27. biological seed coating agents as claimed in claim 26, is characterized in that, in described nutritive element, the weight percent hundred of manganese sulphate is than being 0.3%.
28. biological seed coating agents as claimed in claim 21, is characterized in that, in described nutritive element, the weight percent hundred of zinc sulphate is than being 0.2-0.4%.
29. biological seed coating agents as claimed in claim 28, is characterized in that, in described nutritive element, the weight percent hundred of zinc sulphate is than being 0.3%.
30. biological seed coating agents as claimed in claim 21, is characterized in that, in described nutritive element, the weight percent hundred of copper sulphate is than being 0.12-0.18%.
31. biological seed coating agents as claimed in claim 30, is characterized in that, in described nutritive element, the weight percent hundred of copper sulphate is than being 0.15%.
The preparation method of 32. biological seed coating agents according to any one of claim 1-31, it comprises and each component of the biological seed coating agent according to any one of claim 1-31 being mixed, and obtains described biological seed coating agent.
33. preparation methods as claimed in claim 32, it comprises:
(1) described biocontrol microorganisms is fermented, obtain zymotic fluid;
(2) film forming agent is dissolved in the hot water, obtained film forming agent solution;
(3) described film forming agent solution is mixed with described zymotic fluid, and stir, obtain mixed liquor;
(4) in described mixed liquor, add plant growth regulator, nutritive element, dispersant, thickener, antifreezing agent, preservative, colouring agent, stir, obtain described biological seed coating agent.
The using method of 34. biological seed coating agents according to any one of claim 1-31, it comprises and being contacted with watermelon seed by described biological seed coating agent.
35. usings method as claimed in claim 34, wherein said biological seed coating agent and watermelon seed carry out dressing with the weight ratio of 1:50-1:200.
36. usings method as claimed in claim 35, wherein said biological seed coating agent and watermelon seed carry out dressing with the weight ratio of 1:70-1:150.
37. usings method as claimed in claim 36, wherein said biological seed coating agent and watermelon seed carry out dressing with the weight ratio of 1:100.
38. purposes of biological seed coating agent in the control of watermelon soil-borne disease according to any one of claim 1-31;
Described watermelon soil-borne disease is watermelon blight, watermelon damping off and/or watermelon damping off.
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| CN104542716A (en) * | 2014-12-30 | 2015-04-29 | 天津市工业微生物研究所有限公司 | Novel biological seed coating agent and preparation method thereof |
| CN104430540A (en) * | 2014-12-31 | 2015-03-25 | 吴中区金庭小美华家庭农场 | Environment-friendly seed treating agent for increasing seed germination rate and preparation method thereof |
| CN105316266A (en) * | 2015-12-08 | 2016-02-10 | 领先生物农业股份有限公司 | Bacillus amyloliquefaciens LX-J1 and application thereof |
| CN106577772B (en) * | 2016-12-02 | 2020-04-14 | 青岛蔚蓝生物集团有限公司 | Biological seed coating agent and application thereof |
| CN107557310B (en) * | 2017-05-31 | 2020-11-20 | 北方民族大学 | Bacillus pumilus FE05 and application thereof |
| CN107227270B (en) * | 2017-05-31 | 2020-05-22 | 北方民族大学 | Biological control microbial inoculum for soil-borne diseases, biological control substrate for soil-borne diseases, and preparation method and use method thereof |
| CN109699681B (en) * | 2019-01-28 | 2020-10-23 | 江苏省农业科学院 | Bacillus pumilus ZR3-3 for preventing and treating bacterial diseases and application thereof |
| CN110352958A (en) * | 2019-08-23 | 2019-10-22 | 云南农业大学 | A kind of Bio-seed Coating agent and preparation method thereof |
| CN113881598B (en) * | 2021-10-22 | 2023-01-31 | 仲恺农业工程学院 | Antibacterial and detoxifying composite biocontrol microbial inoculum for relieving continuous cropping of watermelons and application thereof |
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