CN103004884A - Biological watermelon seed coating formulation and preparation and applications thereof - Google Patents
Biological watermelon seed coating formulation and preparation and applications thereof Download PDFInfo
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- CN103004884A CN103004884A CN2012104350414A CN201210435041A CN103004884A CN 103004884 A CN103004884 A CN 103004884A CN 2012104350414 A CN2012104350414 A CN 2012104350414A CN 201210435041 A CN201210435041 A CN 201210435041A CN 103004884 A CN103004884 A CN 103004884A
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Abstract
The invention discloses a biological watermelon seed coating formulation and a preparation method and applications thereof. The biological watermelon seed coating formulation can effectively prevent various soil-borne diseases of watermelon and has the growth-promoting effect on the watermelon, increases the watermelon output remarkably, and meanwhile is free of environment pollution and is low in toxicity and safe. The biological watermelon seed coating formulation is simple in production process, low in production cost, convenient to implement and long in storage period, and is applicable to large-scale industrial production and seed treatment.
Description
Technical field
The present invention relates to a kind of biopesticide preparation, specifically, is a kind of watermelon biological seed coating agent and preparation and application.
Background technology
Watermelon is annual climing property herbaceous plant.Melon pulp is tender and crisp, the sweet succulence of distinguishing the flavor of.Except not fatty and cholesterol, watermelon contains a large amount of glucose, malic acid, fructose, Argine Monohydrochloride, tomatin and the abundant materials such as vitamin C, be the main fruit of relieving summer heat in summer, and the diseases such as treatment ephritis, diabetes and cystitis are had auxiliary curative effect.In addition, its seed oil content reaches 50%, can extract oil, stir-fries and eats or do the cake batching.The watermelon disease has kind more than 10, wherein harm is more serious the climing rot of watermelon, watermelon blight, watermelon anthrax, watermelon virus disease, watermelon powdery mildew, watermelon leaf rot, watermelon epidemic disease, a watermelon root-knot disease etc., the harm of these diseases not only affects the yield of watermelon, also affects quality.The disease that wherein harm such as watermelon blight, watermelon damping off, watermelon damping off are larger is mainly propagated by soil, can carry out effectively preventing by seed is carried out Cotton seeds.
Kind of clothing technology is that of growing up on tradition seed soaking, the basis of seed dressing uses commonplace seed processing technology in the world, is the standardization of seed quality, the modernization of seed processing and the combination of field integrated pest prevention and control in seedling stage.Use kind of a clothing technology that crop seed is processed and have following benefit: (1) with desinsection, sterilization, kill that mouse, sterilization are compound, coordinate plant growth; (2) change seed subenvironment on every side, nutrition and oxygen are provided, promote plant early to germinate, grow fast, early ripe; (3) regulate shape of the seed, size, be beneficial to machine sowing, and break the seed dormancy phase, reach the purpose of continuous sowing etc.Planting the kind clothing agent of adopting in the clothing technology is comprised of active component and non-active ingredient usually.Active component mainly comprises insecticide, bactericide, hormone, fertilizer or beneficial microbe and metabolite thereof etc., is the active ingredient that directly plays a role in kind of the clothing agent.Non-active ingredient refers to film forming agent and the corresponding additive in kind of the clothing agent, to keep kind of the physics of clothing agent and a chemical property, the important component of active component slowly-releasing comprises film forming agent, dispersant, suspending agent, antifreezing agent, warning colouration, defoamer, thickener, preservative etc. in the controlling diaphragm.The composition of non-active ingredient directly affects kind of the quality of clothing agent and a coating effect.Using at present more is the agent of chemical seed clothing, and it has the series of problems such as high malicious high residue and active component lasting period be short.
The Bio-seed Coating agent is the agent of a kind of cenospecies clothing, it is the important component part of biopesticide, its active component is natural products, microorganism live body or its metabolite that can control phytopathogen, mainly comprise microbiocidal (living microorganism and farm antibiotics etc.), plant resource type, the formulation of synthesizing take microbial metabolic products as the guide, genetic engineering bacterial strain etc., can effectively overcome the problems such as the high malicious high residue of chemical seed clothing agent and active component lasting period be short, crop itself is also had certain trophic function, can improve the quality of crop.But the Bio-seed Coating agent, the specific aim of Bio-seed Coating agent that especially has the prevention and elimination of disease and pests function is relatively strong, and different crops needs the Bio-seed Coating agent of difference in functionality according to the difference of its processing intent.
Bacillus (Bacillus spp.) is important plant growth-promoting rhizobacteria (Plant Growth-Promoting Rhizobacteria, PGPR), has a liking for temperature, aerobic, product gemma, and its physiological characteristic is various, and is widely distributed, very easily separates and cultivates.This bacterium is widespread in nature, and is nontoxic to people and animals, free from environmental pollution, can produce multiple antibiotic and enzyme, has broad spectrum antibiotic activity and extremely strong anti-adversity ability.The prominent features such as growth is fast because bacillus has, nutrition is simple and it is heat-resisting to produce, degeneration-resistant gemma, producing, formulation and the survival in environment, surely growing and breeding and make it not only be conducive to biocontrol fungicide, and mass production processes is simple, cost is lower, use conveniently, storage life is long, has now become a kind of desirable biocontrol bacteria.
In the art, the multiple soil-borne disease that can effectively prevent and treat watermelon for comprising is arranged, improve the needs of the new bio type kind clothing agent of the yield of watermelon.
Summary of the invention
The object of the invention is to provide the biological seed coating agent of a kind of, low toxicity nuisanceless to environment, safety, and it not only has growth-promoting functions to watermelon, and can effectively prevent and treat the multiple soil-borne disease of watermelon, improves the yield of watermelon.
The present invention realizes by following technological means:
In one aspect of the invention, a kind of biological seed coating agent is provided, described biological seed coating agent comprises the biocontrol microorganisms zymotic fluid, described biocontrol microorganisms is that bacillus pumilus (Bacillus pumilus) LNXM12(is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC) (No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica), preservation date is on November 02nd, 2009, and preserving number is CGMCC No.3380).
In biological seed coating agent of the present invention, cell concentration can be 10 in the described biocontrol microorganisms zymotic fluid
8More than the cfu/mL.
Biological seed coating agent of the present invention can also comprise plant growth regulator, nutritive element and film forming agent solution, and randomly dispersant, thickener, antifreezing agent, preservative, colouring agent or its combination of at least 2 kinds.
Biological seed coating agent of the present invention can be mixed by following composition according to percentage by weight: biocontrol microorganisms zymotic fluid 10%～30%, 15-25% preferably, more preferably 20%; Plant growth regulator 0.01～0.05%, 0.02-0.04% preferably, more preferably 0.03%; Nutritive element 10～15%, preferably 11-14%, more preferably 12-13%; Film forming agent solution 40%～60%, 45-55% preferably, more preferably 50%; Dispersant 1%～3%, 1.5-2.5% preferably, more preferably 2%; Thickener 0.3%～0.7%, 0.4-0.6% preferably, more preferably 0.5%; Antifreezing agent 3%～8%, preferably 4-7%, more preferably 5-6%; Preservative 0.3%～1.0%, 0.5-0.9% preferably, more preferably 0.7%; Colouring agent 0.5%～1.5%, 0.7-1.3% preferably, more preferably 1.0%; With surplus be water.
In biological seed coating agent of the present invention, the percentage by weight of biocontrol microorganisms zymotic fluid can be 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29% or 30%.
In biological seed coating agent of the present invention, the percentage by weight of plant growth regulator can be 0.01%, 0.02%, 0.03%, 0.04% or 0.05%.
In biological seed coating agent of the present invention, the percentage by weight of nutritive element can be 10%, 11%, 12%, 13%, 14% or 15%.
In biological seed coating agent of the present invention, the percentage by weight of film forming agent solution can be 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59% or 60%.
In biological seed coating agent of the present invention, the percentage by weight of dispersant can be 1%, 1.5%, 2%, 2.5% or 3%.
In biological seed coating agent of the present invention, the percentage by weight of thickener can be 0.3%, 0.4%, 0.5%, 0.6% or 0.7%.
In biological seed coating agent of the present invention, the percentage by weight of antifreezing agent can be 3%, 4%, 5%, 6%, 7% or 8%.
In biological seed coating agent of the present invention, the percentage by weight of preservative can be 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9% or 1.0%.
In biological seed coating agent of the present invention, the percentage by weight of colouring agent can be 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, 1.2%, 1.3%, 1.4% or 1.5%.
In biological seed coating agent of the present invention, cell concentration can be 10 in the described biocontrol microorganisms zymotic fluid
8More than the cfu/mL.
In biological seed coating agent of the present invention, described plant growth regulator can be methyl α-naphthyl acetate;
In biological seed coating agent of the present invention, described nutritive element can be comprised of the following material of percentage by weight: potassium sulphate 1-4%, 2-3% preferably, more preferably 2.5%; Boric acid 0.5-0.7%, 0.55-0.65% preferably, more preferably 0.6%; Manganese sulphate 0.2-0.4%, 0.25-0.35% preferably, more preferably 0.3%; Zinc sulphate 0.1-0.5%, 0.2-0.4% preferably, more preferably 0.3%; With copper sulphate 0.1-0.2%, 0.12-0.18% preferably, more preferably 0.15%.
Wherein, the passable percentage by weight of potassium sulphate is 1%, 1.5%, 2%, 2.5%, 3%, 3.5% or 4%; The percentage by weight of boric acid can be 0.5%, 0.55%, 0.6%, 0.65% or 0.7%; The percentage by weight of manganese sulphate can be 0.2%, 0.25%, 0.3%, 0.35% or 0.4%; The percentage by weight of zinc sulphate can be 0.1%, 0.2%, 0.3%, 0.4% or 0.5%; The percentage by weight of copper sulphate can be 0.1%, 0.11%, 0.12%, 0.13%, 0.14%, 0.15%, 0.16%, 0.17%, 0.18%, 0.19% or 0.2%.
In biological seed coating agent of the present invention, described film forming agent solution can be the mixture of any one or at least two kinds in 3% sodium carboxymethylcellulose and 4% polyvinyl alcohol and 2% shitosan;
In biological seed coating agent of the present invention, described dispersant can be sodium lignin sulfonate, the mixture of any one in neopelex and the calcium lignosulfonate or at least two kinds;
In biological seed coating agent of the present invention, described thickener can be the mixture of any one or at least two kinds in xanthans, gum Arabic and the Magnesiumaluminumsilicate;
In biological seed coating agent of the present invention, described antifreezing agent can be ethylene glycol and/or propane diols;
In biological seed coating agent of the present invention, described preservative can be the mixture of any one or at least two kinds in Sodium Benzoate, potassium sorbate and the sodium salicylate;
In biological seed coating agent of the present invention, described colouring agent can be sarranine or basic rhodamine.
In a second aspect of the present invention, the preparation method such as the described biological seed coating agent of first aspect is provided, comprise the steps:
(1) with bacillus pumilus LNXM12 by fermentation, obtain bacteria concentration 〉=10
8The zymotic fluid of cfu/ml, for subsequent use;
(2) film forming agent is dissolved in hot water, make film forming agent solution.
(3) above-mentioned film forming agent solution is mixed in certain proportion with the zymotic fluid of bacillus pumilus LNXM12, and stir;
(4) in above-mentioned mixed liquor, add respectively a certain proportion of plant growth regulator, nutritive element, dispersant, thickener, antifreezing agent, preservative, colouring agent, stir, obtain described biological seed coating agent.
In a third aspect of the present invention, the using method such as the described biological seed coating agent of first aspect is provided, it comprises described biological seed coating agent is contacted with watermelon seed.
In the using method of described biological seed coating agent of the present invention, described biological seed coating agent and watermelon seed can 1:50-1:200 weight ratio carry out dressing, preferably carry out dressing with the weight ratio of 1:70-1:150, more preferably carry out dressing with the weight ratio of 1:100.
In a fourth aspect of the present invention, provide such as the purposes of the described biological seed coating agent of first aspect in the control of watermelon soil-borne disease.
In the purposes such as the described biological seed coating agent of first aspect of the present invention, described watermelon soil-borne disease is watermelon blight, watermelon damping off and/or watermelon damping off.
In the present invention, soil-borne disease refers to that pathogene such as fungi, bacterium, nematode and virus lives in the soil with invalid body, the disease that causes from crop root or stem infringement crop when condition is suitable.
Beneficial effect of the present invention is as follows:
(1) biological seed coating agent of the present invention can effectively be prevented and treated the multiple soil-borne disease of watermelon, and watermelon is had growth-promoting functions, can significantly improve the yield of watermelon;
(2) biological seed coating agent of the present invention is nuisanceless to environment, is the biological seed coating agent of a kind of low toxicity, safety;
(3) biological seed coating agent of the present invention is used conveniently, and the storage life is long, is suitable for large-scale seed treatment;
(4) biological seed coating agent producing process of the present invention is simple, and production cost is lower, is suitable for large-scale industrial production.
Embodiment
Below further describe the present invention by embodiment, as known by the technical knowledge, the present invention also can describe by other the scheme that does not break away from the technology of the present invention feature, thus all within the scope of the present invention or the change that is equal in the scope of the invention all be included in the invention.
Embodiment 1:
The agent of the present embodiment kind clothing is formulated with following weight ratio by each composition:
Biocontrol microorganisms zymotic fluid 30%, plant growth regulator 0.02%, nutritive element 10%, film forming agent solution 50%(3% carboxymethylcellulose sodium solution), thickener 0.3%(xanthans), dispersant 2.5%(sodium lignin sulfonate), antifreezing agent 5%(ethylene glycol), preservative 0.5%(Sodium Benzoate), colouring agent 0.5%(sarranine), all the other are water.
Wherein the percentage by weight of nutritive element consists of potassium sulphate 4%, boric acid 0.5%, and manganese sulphate 0.4%, zinc sulphate 0.1%, copper sulphate 0.2%, all the other are water.
Embodiment 2:
The agent of the present embodiment kind clothing is formulated with following weight ratio by each composition:
Biocontrol microorganisms zymotic fluid 20%, plant growth regulator 0.05%, nutritive element 15%, film forming agent solution 40%(4% poly-vinyl alcohol solution), thickener 0.7%(gum Arabic), dispersant 2.5%(sodium lignin sulfonate), antifreezing agent 5%(ethylene glycol), preservative 0.3%(potassium sorbate), colouring agent 0.5%(sarranine), all the other are water.
Wherein the percentage by weight of nutritive element consists of potassium sulphate 1%, boric acid 0.7%, and manganese sulphate 0.2%, zinc sulphate 0.5%, copper sulphate 0.1%, all the other are water.
Embodiment 3:
The agent of the present embodiment kind clothing is formulated with following weight ratio by each composition:
Biocontrol microorganisms zymotic fluid 30%, plant growth regulator 0.03%, nutritive element 12%, film forming agent solution 45%(2% chitosan solution), thickener 0.5%(aluminium-magnesium silicate), dispersant 3%(neopelex), antifreezing agent 3%(propane diols), preservative 0.7%(sodium salicylate), colouring agent 1%(basic rhodamine), all the other are water.
Wherein the percentage by weight of nutritive element consists of potassium sulphate 2%, boric acid 0.6%, and manganese sulphate 0.3%, zinc sulphate 0.3%, copper sulphate 0.15%, all the other are water.
Embodiment 4
The agent of the present embodiment kind clothing is formulated with following weight ratio by each composition:
Biocontrol microorganisms zymotic fluid 10%, plant growth regulator 0.04%, nutritive element 11%, film forming agent solution 60%(3% carboxymethylcellulose sodium solution), thickener 0.4%(xanthans), dispersant 1%(calcium lignosulfonate), antifreezing agent 4%(ethylene glycol), preservative 0.9%(Sodium Benzoate), colouring agent 1.5%(sarranine), all the other are water.
Wherein the percentage by weight of nutritive element consists of potassium sulphate 3%, boric acid 0.55%, and manganese sulphate 0.35%, zinc sulphate 0.2%, copper sulphate 0.19%, all the other are water.
Embodiment 5
The agent of the present embodiment kind clothing is formulated with following weight ratio by each composition:
Biocontrol microorganisms zymotic fluid 25%, plant growth regulator 0.01%, nutritive element 13%, film forming agent solution 45%(2% shitosan), thickener 0.6%(Magnesiumaluminumsilicate), dispersant 2%(neopelex), antifreezing agent 8%(ethylene glycol), preservative 1%(sodium salicylate), colouring agent 1.2%(sarranine), surplus is water
Wherein the percentage by weight of nutritive element consists of potassium sulphate 2%, boric acid 0.65%, and manganese sulphate 0.25%, zinc sulphate 0.4%, copper sulphate 0.13%, all the other are water.
Embodiment 6
The agent of the present embodiment kind clothing is formulated with following weight ratio by each composition:
Biocontrol microorganisms zymotic fluid 15%, plant growth regulator 0.03%, nutritive element 14%, film forming agent solution 55%(2% shitosan), thickener 0.4%(Magnesiumaluminumsilicate), dispersant 1.5%(sodium lignin sulfonate), antifreezing agent 7%(ethylene glycol), preservative 0.6%(sodium salicylate), colouring agent 0.8%(sarranine), surplus is water
Wherein the percentage by weight of nutritive element consists of potassium sulphate 3%, boric acid 0.6%, and manganese sulphate 0.3%, zinc sulphate 0.3%, copper sulphate 0.17%, all the other are water.
The preparation method is identical for the clothing agent of above-described embodiment kind, at first with bacillus pumilus LNXM12 by fermentation, obtains bacteria concentration 〉=10
8The zymotic fluid of cfu/ml, for subsequent use; Film forming agent is dissolved in hot water, make film forming agent solution; Above-mentioned film forming agent solution is mixed with corresponding proportion with the zymotic fluid of bacillus pumilus LNXM12, and stir; Add respectively plant growth regulator, nutritive element, dispersant, thickener, antifreezing agent, preservative, the colouring agent of corresponding proportion in the above-mentioned mixed liquor, stir, namely get the biological seed coating agent of inventing.
Prepared kind of clothing agent and watermelon seed carry out dressing with the weight ratio of 1:50-1:200, can effectively prevent and treat the multiple soil-borne disease of watermelon.
To develop biological seed coating agent action effect in actual applications in order investigating, to do pot experiment as follows.
Test example 1:
This test example is to study biological seed coating agent and comes into force really to the diseases prevention of watermelon blight and to the short of watermelon, has done following test:
Kind clothing agent by embodiment 1 described prescription preparation is carried out dressing with the weight ratio of 1:200 to watermelon seed, establish simultaneously two groups of contrasts, the first is carried out dressing to watermelon seed with the weight ratio of 1:50 with the U.S. Seedvax suspension seed-coating agent of chemical pesticide 10% good fortune (planting clothing agent Co., Ltd by the farming of Jiangsu China produces); It two is to process watermelon seed with clear water.Sow in the usual way after capsuled seed dried and filling sterilization soil and be mixed with in the flowerpot of watermelon blight pathogen (Fusarium oxysporum watermelon transformant).Grow after one month, check the afterwards incidence of watermelon of different modes processing, and the situation of growing, the result is as shown in table 1:
Growth and the incidence of watermelon after table 1. different modes is processed
As shown in Table 1, no matter at germination rate or at plant height, fresh weight all is better than the watermelon that chemical pesticide and clear water are processed on the growth indexes such as root is long, illustrate that biological seed coating agent of the present invention can effectively promote watermelon growing by the watermelon of biological seed coating agent dressing of the present invention.Relatively each disease index of processing as can be known, the lightest by the watermelon morbidity that biological seed coating agent is processed, the preventive effect of watermelon blight is reached 76.74%, be better than chemical pesticide and process.Illustrate that biological seed coating agent of the present invention has good control efficiency to watermelon blight.
Test example 2:
This test example is to study biological seed coating agent and comes into force really to the diseases prevention of watermelon damping off and to the short of watermelon, has done following test:
Kind clothing agent by embodiment 3 described prescription preparations is carried out dressing with the weight ratio of 1:150 to watermelon seed, establish simultaneously two groups of contrasts, the first is carried out dressing to watermelon seed with the weight ratio of 1:50 with the U.S. Seedvax suspension seed-coating agent of chemical pesticide 10% good fortune (planting clothing agent Co., Ltd by the farming of Jiangsu China produces); It two is to process watermelon seed with clear water.Sow in the usual way after capsuled seed dried and filling sterilization soil and be mixed with in the flowerpot of watermelon damping off pathogen (Rhizoctonia solani Kuhn).Grow after one month, check the afterwards incidence of watermelon of different modes processing, and the situation of growing, the result is as shown in table 2:
Growth and the incidence of watermelon after table 2. different modes is processed
As shown in Table 2, no matter at germination rate or at plant height, fresh weight all is better than the watermelon that chemical pesticide and clear water are processed on the growth indexes such as root is long, illustrate that biological seed coating agent of the present invention can effectively promote watermelon growing by the watermelon of biological seed coating agent dressing of the present invention.Relatively each disease index of processing as can be known, the lightest by the watermelon morbidity that biological seed coating agent is processed, the preventive effect of watermelon damping off is reached 78.20%, be better than preventive effect and be 35.54% chemical pesticide and process.Illustrate that biological seed coating agent of the present invention has good control efficiency to the watermelon damping off.
Test example 3
This test example is to study biological seed coating agent and comes into force really to the diseases prevention of watermelon damping off and to the short of watermelon, has done following test:
Kind clothing agent by embodiment 2 described prescription preparations is carried out dressing with the weight ratio of 1:100 to watermelon seed, establish simultaneously two groups of contrasts, the first is carried out dressing to watermelon seed with the weight ratio of 1:50 with the U.S. Seedvax suspension seed-coating agent of chemical pesticide 10% good fortune (planting clothing agent Co., Ltd by the farming of Jiangsu China produces); It two is to process watermelon seed with clear water.Sow in the usual way after capsuled seed dried and filling sterilization soil and be mixed with in the flowerpot of watermelon damping off pathogen (melon and fruit pythium spp).Grow after one month, check the afterwards incidence of watermelon of different modes processing, and the situation of growing, the result is as shown in table 3:
Growth and the incidence of watermelon after table 3. different modes is processed
As shown in Table 3, no matter at germination rate or at plant height, fresh weight all is better than the watermelon that chemical pesticide and clear water are processed on the growth indexes such as root is long, illustrate that biological seed coating agent of the present invention can effectively promote watermelon growing by the watermelon of biological seed coating agent dressing of the present invention.Relatively each disease index of processing as can be known, the lightest by the watermelon morbidity that biological seed coating agent is processed, the preventive effect of watermelon damping off is reached 77.32%, be better than preventive effect and be 35.75% chemical pesticide and process.Illustrate that biological seed coating agent of the present invention has good control efficiency to the watermelon damping off.
Applicant's statement, the present invention illustrates detailed features of the present invention and method by above-described embodiment, but the present invention is not limited to above-mentioned detailed features and method, does not mean that namely the present invention must rely on above-mentioned detailed structure feature and method could be implemented.The person of ordinary skill in the field should understand, any improvement in the present invention to the increase of the equivalence replacement of the selected component of the present invention and helper component, the selection of concrete mode etc., all drops within protection scope of the present invention and the open scope.
Claims (10)
1. biological seed coating agent, it is characterized in that, described biological seed coating agent comprises the biocontrol microorganisms zymotic fluid, and described biocontrol microorganisms is that bacillus pumilus (Bacillus pumilus) LNXM12(preserving number is CGMCCNo.3380), preferably, in the described biocontrol microorganisms zymotic fluid cell concentration 10
8More than the cfu/mL.
2. biological seed coating agent as claimed in claim 1, it is characterized in that, described biological seed coating agent also comprises plant growth regulator, nutritive element and film forming agent solution, and randomly dispersant, thickener, antifreezing agent, preservative, colouring agent or its combination of at least 2 kinds.
3. biological seed coating agent as claimed in claim 1 or 2 is characterized in that, is grouped into by following one-tenth according to percentage by weight:
Biocontrol microorganisms zymotic fluid 10%～30%, 15-25% preferably, more preferably 20%;
Plant growth regulator 0.01～0.05%, 0.02-0.04% preferably, more preferably 0.03%;
Nutritive element 10～15%, preferably 11-14%, more preferably 12-13%;
Film forming agent solution 40%～60%, 45-55% preferably, more preferably 50%;
Dispersant 1%～3%, 1.5-2.5% preferably, more preferably 2%;
Thickener 0.3%～0.7%, 0.4-0.6% preferably, more preferably 0.5%;
Antifreezing agent 3%～8%, preferably 4-7%, more preferably 5-6%;
Preservative 0.3%～1.0%, 0.5-0.9% preferably, more preferably 0.7%;
Colouring agent 0.5%～1.5%, 0.7-1.3% preferably, more preferably 1.0%; With
Surplus is water.
4. such as each described biological seed coating agent among the claim 1-3, it is characterized in that, described plant growth regulator is methyl α-naphthyl acetate; Described film forming agent solution is 3% carboxymethylcellulose sodium solution and 4% poly-vinyl alcohol solution, the mixture of any one in 2% chitosan solution or at least two kinds; Described dispersant is sodium lignin sulfonate, the mixture of any one in neopelex and the calcium lignosulfonate or at least two kinds; Described thickener is the mixture of any one or at least two kinds in xanthans, gum Arabic and the Magnesiumaluminumsilicate; Described antifreezing agent can be ethylene glycol and/or propane diols; Described preservative is the mixture of any one or at least two kinds in Sodium Benzoate, potassium sorbate and the sodium salicylate; Described colouring agent is sarranine or basic rhodamine.
5. such as each described biological seed coating agent among the claim 1-4, it is characterized in that, the chief component of described nutritive element take and percentage by weight as:
Potassium sulphate 1-4%, 2-3% preferably, more preferably 2.5%;
Boric acid 0.5-0.7%, 0.55-0.65% preferably, more preferably 0.6%;
Manganese sulphate 0.2-0.4%, 0.25-0.35% preferably, more preferably 0.3%;
Zinc sulphate 0.1-0.5%, 0.2-0.4% preferably, more preferably 0.3%;
Copper sulphate 0.1-0.2%, 0.12-0.18% preferably, more preferably 0.15%;
All the other are water.
6. such as the preparation method of each described biological seed coating agent among the claim 1-5, it comprises each component of each described biological seed coating agent among the claim 1-5 is mixed, obtains described biological seed coating agent.
7. preparation method as claimed in claim 6, it comprises:
(1) with described biocontrol microorganisms fermentation, obtains zymotic fluid;
(2) film forming agent is dissolved in hot water, make film forming agent solution;
(3) described film forming agent solution is mixed with described zymotic fluid, and stir, obtain mixed liquor;
(4) in described mixed liquor, add plant growth regulator, nutritive element, dispersant, thickener, antifreezing agent, preservative, colouring agent, stir, obtain described biological seed coating agent.
8. such as the using method of each described biological seed coating agent among the claim 1-5, it comprises described biological seed coating agent is contacted with watermelon seed.
9. using method as claimed in claim 8, wherein said biological seed coating agent and watermelon seed carry out dressing with the weight ratio of 1:50-1:200, preferably carry out dressing with the weight ratio of 1:70-1:150, more preferably carry out dressing with the weight ratio of 1:100.
10. such as the purposes of each described biological seed coating agent in the control of watermelon soil-borne disease among the claim 1-5, preferably, described watermelon soil-borne disease is watermelon blight, watermelon damping off and/or watermelon damping off.
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| CN201210435041.4A CN103004884B (en) | 2012-11-01 | 2012-11-01 | Watermelon biological seed coating agent and Synthesis and applications thereof |
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| CN104430540A (en) * | 2014-12-31 | 2015-03-25 | 吴中区金庭小美华家庭农场 | Environment-friendly seed treating agent for increasing seed germination rate and preparation method thereof |
| CN104542716A (en) * | 2014-12-30 | 2015-04-29 | 天津市工业微生物研究所有限公司 | Novel biological seed coating agent and preparation method thereof |
| CN105316266A (en) * | 2015-12-08 | 2016-02-10 | 领先生物农业股份有限公司 | Bacillus amyloliquefaciens LX-J1 and application thereof |
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| CN104542716A (en) * | 2014-12-30 | 2015-04-29 | 天津市工业微生物研究所有限公司 | Novel biological seed coating agent and preparation method thereof |
| CN104430540A (en) * | 2014-12-31 | 2015-03-25 | 吴中区金庭小美华家庭农场 | Environment-friendly seed treating agent for increasing seed germination rate and preparation method thereof |
| CN105316266A (en) * | 2015-12-08 | 2016-02-10 | 领先生物农业股份有限公司 | Bacillus amyloliquefaciens LX-J1 and application thereof |
| CN106577772A (en) * | 2016-12-02 | 2017-04-26 | 青岛蔚蓝生物集团有限公司 | Novel biological seed coating and application thereof |
| CN106577772B (en) * | 2016-12-02 | 2020-04-14 | 青岛蔚蓝生物集团有限公司 | Biological seed coating agent and application thereof |
| CN107227270A (en) * | 2017-05-31 | 2017-10-03 | 北方民族大学 | A kind of biocontrol bacteria in soil-borne diseases agent, a kind of soil-borne disease biological control matrix and preparation method thereof and application method |
| CN107557310A (en) * | 2017-05-31 | 2018-01-09 | 北方民族大学 | A kind of bacillus pumilus FE05 and its application |
| CN107227270B (en) * | 2017-05-31 | 2020-05-22 | 北方民族大学 | Biological control microbial inoculum for soil-borne diseases, biological control substrate for soil-borne diseases, and preparation method and use method thereof |
| CN107557310B (en) * | 2017-05-31 | 2020-11-20 | 北方民族大学 | Bacillus pumilus FE05 and application thereof |
| CN109699681A (en) * | 2019-01-28 | 2019-05-03 | 江苏省农业科学院 | One plant of prevention and treatment bacteriosis bacillus pumilus ZR3-3 and its application |
| CN110352958A (en) * | 2019-08-23 | 2019-10-22 | 云南农业大学 | A kind of Bio-seed Coating agent and preparation method thereof |
| CN113881598A (en) * | 2021-10-22 | 2022-01-04 | 仲恺农业工程学院 | Antibacterial and detoxifying composite biocontrol microbial inoculum for relieving continuous cropping of watermelons and application thereof |
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