CN107721713A - A kind of microbial compound bacterial fertilizer and preparation method and application - Google Patents
A kind of microbial compound bacterial fertilizer and preparation method and application Download PDFInfo
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- CN107721713A CN107721713A CN201610665832.4A CN201610665832A CN107721713A CN 107721713 A CN107721713 A CN 107721713A CN 201610665832 A CN201610665832 A CN 201610665832A CN 107721713 A CN107721713 A CN 107721713A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- Pest Control & Pesticides (AREA)
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- Inorganic Chemistry (AREA)
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Abstract
The invention discloses a kind of microbial compound bacterial fertilizer, and it includes the component of following parts by weight：5~10 parts of 5~25 parts of complex microorganism, 65~95 parts of compound nutritional carrier, 0.5~2 part of EDTA metallo-chelates and diatomite.The invention also discloses the preparation method of the microbial compound bacterial fertilizer and its application in soil improvement and promotion crop growth.Compared with prior art, this dispensing collaboration of the invention plays a role, can effectively improved soil, loose hardened, balanced nutrient, increase fertility of land, activating soil composition, improve soil water-reataining fertilizer-keeping ability and promote crop yield.Meanwhile simple production process of the present invention, it is convenient that raw material obtains, and it is cheap that dispensing comes from agriculture and industry waste cost, safe operation not high to equipment and site requirements, and application effect is notable.Both can be mass produced with enterprise, again can personal or peasant household oneself prepare use.
Description
Technical field
The invention belongs to microbial manure technical field, and in particular to a kind of microbial compound bacterial fertilizer and preparation method thereof with
Using.
Background technology
Because chemical fertilizer can quickly promote plant growth and increase crop yield, the long-term use of chemistry of peasant in China
Fertilizer, and organic fertilizer is insufficient, causes all kinds of nutritional ingredients out of proportion, soil physico-chemical property and soil microflora is not by
With the destruction of degree, the quality of agricultural product is have impact on to a certain extent.Crop preventing and treating pest and disease damage mainly using chemicals and
Toxic pesticides, in being manured into soil or on crops, long-term use of agricultural chemicals, pest and disease damage is easily caused to produce drug resistance.Chemical fertilizer
Administration would generally break colony balance in soil micro-ecosystem system, the generation of pest and disease damage can be further resulted on the contrary.Many institute's weeks
Know, China has large area salt affected soil, and alkaline land soil nutrient content is low, and saline and alkaline degree is high, is not suitable with and carries out agricultural life
Production.Although at present physics and chemical method can be used to administer salt affected soil, cost is too high, is not suitable with large-area applications and arrives
In practice.
People began to focus in recent years is applied in soil improvement engineering using beneficial microbe, substantially improves soil knot
Structure, water conservation, fertilizer-preserving ability are improved, promote the largely necessary element and trace element such as crop absorbed nitrogen, phosphorus, potassium, reaching makes work
Thing growth-promoting increases production purpose.Microbial bacterial agent has become the future of agriculture sustainable development as a kind of environmental friendly fertilizer
One of important directions.Research to microbial bacterial agent at present focus mostly on fixed nitrogen, decompose soil organic substance and slightly solubility mineral,
The fields such as disease-resistant and stimulation plant growth, Root Symbiont bacterium.Despite the presence of many microbial bacterial agents, but general lack of Black Liquor with Efficient Bacteria
Strain, microorganism resource lack, and the strain of many producers is all utilizing same bacterial strain, while increases for soil improvement, promotion crop
The microbial bacterial agent of production is rarely reported.
Potassium, silicon in bacillusmusilaginosiengineering decomposable asymmetric choice net mineral, can also decomposite the phosphorus in apatite, with Potassium release and admittedly
Nitrogen function, while organic acid, amino acid, polysaccharide, hormone etc. can be produced during growth and breeding and be advantageous to plant absorption and profit
Material.After being bred in soil, plant growth substance and a variety of enzymes are secreted, to strengthen resistance of the crop to some diseases
Power；Also the growth of other pathogens is suppressed.
Bacillus amyloliquefaciens are a kinds in bacillus, have the production energy of stronger secondary metabolite
Power, auxin can be secreted, promote plant growth；Can secretion of phytase and organic acid, degrade soil in phytic acid and mineral
Phosphorus；A variety of antibacterial related metabolites can be produced, all there is strong inhibitory action to fungi and bacterium.
Edible fungus culturing waste material is to plant the residue that edible mushroom is left, general rich in a large amount of foods due to planting edible mushroom
With the residue of bacterium, such as the residual fragment or root system of mushroom, these are all rich in protein and glucide, are used as microorganism and answer
The main carriers of bacterium to be closed, can not only be made in microbial bacterial agent, microorganism growth content is maintained, and the shelf-life significantly improves,
Nutritional ingredient contained by edible fungus culturing waste material can directly absorb for plant simultaneously.Using Chelating state metallic element,
Can there are good stability and compatibility when being compounded with other elements, be absorbed beneficial to crop.
Complex microorganism produces synergy in soil, complimentary to one another, effectively improved soil structure, improves soil fertilizer
Power, soil moisture and nutrition are kept, reduce soil pH value and salt content, promote crop absorbed nitrogen, phosphorus, potassium etc. largely must element
And middle trace element, reaching makes promoting crop growth increase production purpose.
The content of the invention
The technical problem to be solved in the present invention is to provide one kind can improve soil environment, and and can promotes the micro- life of crop yield
Thing composite bacterial fertilizer, to solve the problems such as cost existing for prior art is higher and ineffective.
The present invention also technical problems to be solved are to provide the preparation method of mentioned microorganism composite bacterial fertilizer.
Last technical problems to be solved of the invention are to provide the application of mentioned microorganism composite bacterial fertilizer.
In order to solve the above technical problems, the technical solution adopted by the present invention is as follows：
A kind of microbial compound bacterial fertilizer, it includes the component of following parts by weight：
Wherein, it is preferably as follows the component of parts by weight：
Further, it is preferably as follows the component of parts by weight：
Wherein, described complex microorganism includes bacillus subtilis, bacillusmusilaginosiengineering and bacillus amyloliquefaciens.
Described bacillus subtilis, its Classification And Nomenclature are Bacillus subtilis, and bacterial strain number is XK-1, preservation
In China typical culture collection center, address is Wuhan University of Wuhan, China city, postcode 430072, and deposit number is
CCTCC M 2016264, preservation date are on May 16th, 2016.The bacillus subtilis is that in December, 2014 is southern from Jiangsu Province
Screening obtains in the soil of Jing Shi Pukou Districts.
Described bacillusmusilaginosiengineering, its Classification And Nomenclature are Bacillus mucilaginosus, and bacterial strain number is XK-2,
China typical culture collection center has been preserved in it, address is Wuhan University of Wuhan, China city, postcode 430072, deposit number
For CCTCC M 2016265, preservation date is on May 16th, 2016.The bacillusmusilaginosiengineering is in January, 2015 from Jiangsu Province
Screening obtains in Pukou area's soil.
Described bacillus amyloliquefaciens, its Classification And Nomenclature are Bacillus amyloliquefaciens, and bacterial strain number is
XK-3, China typical culture collection center is preserved in, address is Wuhan University of Wuhan, China city, postcode 430072, is protected
It is CCTCC M 2016266 to hide numbering, and preservation date is on May 16th, 2016.The bacillus amyloliquefaciens be in January, 2015 from
Screening obtains in the soil of Nanjing Pukou District.
Wherein, in complex microorganism, the total plate count of bacillus subtilis is 2.0 × 109~7.0 × 109Cfu/g, it is excellent
Select 3.5 × 109cfu/g；The total plate count of bacillusmusilaginosiengineering is 1.5 × 109~5.0 × 109Cfu/g, preferably 2.5 ×
109cfu/g；The total plate count of bacillus amyloliquefaciens is 0.5 × 109~2.5 × 109Cfu/g, preferably 1.0 × 109cfu/g。
Wherein, described compound nutritional carrier is by mass ratio 3~4：1~3：2~3：1~2：0.5~2：0.5~1 food
It is mixed to get with bacteria cultivation waste material, brid guano, sawdust, stalk, humic acid and konjaku flour after drying and crushing is sieved.
Described edible fungus culturing waste material is provided by Nanjing Liuhe District farm, and main nutrient composition is：Full nitrogen
6.7~10.3g/kg；0.051~0.073g/kg of full phosphorus；0.044~0.147g/kg of full potassium；The content of organic matter be 267.1~
762.4g/kg；Effective nitrogen content is 377~513mg/kg；PH 5.35~6.75.
Above-mentioned mass-energy density preferably utilizes each carrier, beneficial to soil nutrient is adjusted, keeps the growth of microbial composite bacteria,
Nutritional ingredient contained by carrier is easier to crop absorption.
Wherein, described EDTA- metallo-chelates are by EDTA-Fe, EDTA-Cu, EDTA-Zn, EDTA-Mn and EDTA-Mg
With 2~3：1~2：3~4：0.5~2：0.5~2 mass ratio mixes, and preferred mass ratio is 3：2：3：1：1.
Above-mentioned mass ratio can better meet the trace element needed for plant growth and improve the nutrition of lean soil
Composition.
Wherein, described diatomite crosses 200~400 mesh sieves, preferably crosses 350 mesh sieves；Wherein, in diatomite, SiO2Matter
It is 70~75% to measure percentage.
The fineness of 350 mesh, 70~75% SiO2Content causes microorganism not go to utilize the phosphorus and potassium in fertilizer, can be more
Good preservation.
Wherein, described microbial compound bacterial fertilizer is pulvis.
The preparation method of mentioned microorganism composite bacterial fertilizer, it comprises the following steps：
(1) Bacillus globigii spores powder, bacillusmusilaginosiengineering conidia powder and bacillus amyloliquefaciens conidia powder are pressed
After formula ratio mixing, complex microorganism is obtained, and effective total plate count is not less than 5.0 × 10 in every gram of complex microorganism9cfu；
(2) by formula after edible fungus culturing waste material, brid guano, sawdust, stalk, humic acid and konjaku flour drying and crushing being sieved
Amount is well mixed, in 0.35~1.40kg/cm2After high pressure steam sterilization 15~45 minutes, compound nutritional carrier is obtained；
(3) obtained after being well mixed EDTA-Fe, EDTA-Cu, EDTA-Zn, EDTA-Mn and EDTA-Mg according to formula ratio
EDTA- metallo-chelates are standby；
(4) by the compound nutritional carrier being prepared in the complex microorganism being prepared in step (1), step (2), step
Suddenly after the diatomite after the EDTA- metallo-chelates being prepared in (3) and sieving is well mixed by formula ratio, hydroxide is passed through
Sodium solution or hydrochloric acid solution regulation pH are 5.0~7.0, produce microbial compound bacterial fertilizer.
In step (1), Bacillus globigii spores powder, bacillusmusilaginosiengineering conidia powder and bacillus amyloliquefaciens spore
Powder is prepared as follows obtaining：
Inclined-plane culture：Bacillus subtilis, bacillusmusilaginosiengineering and bacillus amyloliquefaciens original strain are existed respectively
It is inoculated under aseptic condition on slant medium, 24~48h is cultivated under the conditions of 28 ± 2 DEG C；
Shaking table culture：Strain after inclined-plane culture is aseptically inoculated in seed culture medium respectively, in pH7.0
Under the conditions of~7.2,28 ± 2 DEG C, 160~200r/min shaking table cultures, 12~18h；
Fermentation tank culture：Strain after shaking table culture is aseptically inoculated in liquid fermentation medium respectively,
In pH 7.0~7.2, throughput is 0.8~1.0vvm, and tank pressure is maintained at 0.02~0.03MPa, under the conditions of 28 ± 2 DEG C, culture
After 48~72h, viable count is more than 1.0 × 109Cfu/mL, 80% thalline turn into gemma tank at present, obtain ferment product；
Prepare conidia powder：After ferment product is concentrated, by 2~5：8~5 mass ratioes are prepared into diatomite combination drying
Each bacterial spore powder.
Wherein,
Described slant medium is LB culture mediums；
The formula of described seed culture medium is：Glucose 10g/L, tryptone 15g/L, potassium dihydrogen phosphate 1g/L, sulphur
7.0~7.4,121 DEG C of high pressure steam sterilization 15min of sour magnesium 0.2g/L, pH；
The formula of described liquid fermentation medium is：Glucose 10g/L, ammonium sulfate 15g/L, beancake powder 5g/L, phosphoric acid
7.0~7.4,121 DEG C of high pressure steam sterilization 15min of potassium dihydrogen 0.5g/L, magnesium sulfate 0.2g/L, pH.
Application of the mentioned microorganism composite bacterial fertilizer in soil improvement and promotion crop growth is also in the guarantor of the present invention
Within the scope of shield.
Wherein, application process is：Using the microbial compound bacterial fertilizer described in claim 1 as crop Special basal fertilizer, dosage
For 50~100kg/ mus.
Wherein, application process is：Base manure is aided in using the microbial compound bacterial fertilizer described in claim 1 as crop, is coordinated
Chemical fertilizer uses, and dosage is 10~15kg/ mus.
Application process：
(1) can ditch spread, spread manuer in holes, ground is spread fertilizer over the fields；
(2) forbid directly to be used in mixed way with bactericide；
(3) deposit at shady and cool dry.
Beneficial effect：
Compared with prior art, the invention has the characteristics that and advantage：
Bacillusmusilaginosiengineering can decompose material containing potassium in soil in the present invention, have Potassium release and fixed nitrogen function, while energy
The material that organic acid, amino acid, polysaccharide, hormone etc. are advantageous to plant absorption and utilized is produced during growth and breeding.Jelly
Potassium content more than 33% in sample bacillus thalline ash content, endobacillary potassium is after thalline death, separate out, can be planted again
Thing directly absorbs.
Bacillus amyloliquefaciens have the production capacity of stronger secondary metabolite in the present invention, can produce a variety of suppressions
The related metabolite of bacterium, all has strong inhibitory action to fungi and bacterium；Can secretion of phytase and organic acid, degraded soil
Phytic acid and mineral phosphorus in earth.
Bacillus subtilis can produce new-type fertilizer synergist gamma-polyglutamic acid in the present invention, and it is high-molecular compound,
With very strong water imbibition and water-retaining property, play a part of keeping soil moisture and chesson, while promote crops to battalion
Foster absorption, fertile synergy, increasing yield and improving quality, enhancing crop disease-resistant ability and other effects are saved, and there is good slow releasing function to fertilizer,
Environmental pollution caused by the excessive or administration improper grade of chemical fertilizer can be reduced.Konjaku flour, which also has, simultaneously has very strong water imbibition and water conservation
Property, the two can cooperate with and play a role.Organic nutrient in edible fungus culturing waste material and chicken manure for microorganism provide living environment with
Nutriment, stimulate it to produce active material, ensure the survival rate and effect of bacterial strain, promote plant growth, and can increase
Soil nutrient, improved soil structure, improve chemical fertilizer utilization ratio；The middle trace element of Chelating state is reasonably combined, effectively facilitates crop
Volume increase, and improve soil environment.
In the present invention dispensing collaboration play a role, can effectively improved soil, loose hardened, balanced nutrient, increase fertility of land,
Activating soil composition, improve soil water-reataining fertilizer-keeping ability and promote crop yield.
Simple production process of the present invention, it is convenient that raw material obtains, and it is cheap that dispensing comes from agriculture and industry waste cost, to equipment
And site requirements is not high, safe operation, application effect is notable.Both can be mass produced with enterprise, again can personal or peasant household
Oneself prepares use.
Embodiment
According to following embodiments, the present invention may be better understood.It is however, as it will be easily appreciated by one skilled in the art that real
Apply the content described by example and be merely to illustrate the present invention, without should be also without limitation on sheet described in detail in claims
Invention.
Embodiment 1
A kind of microbial compound bacterial fertilizer, it is made up of the component of following weight：15 parts of complex microorganism, compound nutritional carrier
75 parts, 1 part of EDTA- metallo-chelates, 9 parts of diatomite, be well mixed；
Wherein, effective viable bacteria total content 7.0 × 10 in the composite bacterial fertilizer9cfu/g.Wherein contain bacillus subtilis
3.5×109Cfu/g, bacillusmusilaginosiengineering 2.5 × 109Cfu/g and bacillus amyloliquefaciens 1.0 × 109cfu/g。
The preparation method of mentioned microorganism composite bacterial fertilizer is as follows：
Step 1, the preparation of complex microorganism：
(1), inclined-plane culture：Respectively by bacillus subtilis XK-1, bacillusmusilaginosiengineering XK-2, bacillus amyloliquefaciens
XK-3 original strains are aseptically inoculated on slant medium, and 24-48h is cultivated under the conditions of 28 ± 2 DEG C；
(2), shaking table culture：The strain that step (1) is cultivated aseptically is inoculated in seed culture medium respectively,
PH 7.0-7.2, under the conditions of 28 ± 2 DEG C, 160-200r/min shaking table cultures 12-18h；
(3), fermentation tank culture：The strain that step (2) is cultivated aseptically is inoculated in liquid fermentation and culture respectively
In base, 0.02-0.03MPa is maintained at, under the conditions of 28 ± 2 DEG C in pH 7.0-7.2, throughput 0.8-1.0vvm, tank pressure, training
After supporting 48-72h, viable count is more than 1.0 × 109Cfu/mL, 80% thalline turn into gemma tank at present, obtain ferment product；
(4), by the zymotic fluid obtained in step (3) it is concentrated after, be prepared into each bacterium by proper proportion and carrier drying
Conidia powder；
Wherein, slant medium is LB culture mediums in step 1；
Seed culture based formulas is as follows：Glucose 10g/L, tryptone 15g/L, potassium dihydrogen phosphate 1g/L, magnesium sulfate
0.2g/L, pH 7.0-7.4,121 DEG C of high pressure steam sterilization 15min；
Liquid fermentation medium formula is as follows：Glucose 10g/L, ammonium sulfate 15g/L, beancake powder 5g/L, potassium dihydrogen phosphate
0.5g/L, magnesium sulfate 0.2g/L, pH 7.0-7.4,121 DEG C of high pressure steam sterilization 15min.
Step 2, the preparation of nutrition complex carrier：By edible fungus culturing waste material, chicken manure, sawdust, crop material, humic acid,
Powder after the sieving of konjaku flour drying and crushing presses 4：2：2：1：0.5：0.5 weight is than mixing, HTHP moist heat sterilization 1h,
It is dried for standby.
Step 3, the preparation of EDTA- metallo-chelates：By EDTA-Fe, EDTA-Cu, EDTA-Zn, EDTA-Mn and EDTA-
Mg is according to 3：2：3：1：1 weight is than mixing.
Step 4, the preparation of microbial compound bacterial fertilizer：Complex microorganism, nutrition complex carrier, silicon are weighed by weight proportion
Diatomaceous earth, EDTA- metallo-chelates, soil improvement composite bacteria agent is produced after mixing, keep composite bacteria agent pH in 5.0-7.0.Implement
Example 2
A kind of microbial compound bacterial fertilizer, it is made up of the component of following parts by weight：18 parts of composite bacteria agent, 75 parts of carrier, silicon
6 parts of diatomaceous earth, 1 part of EDTA- metallo-chelates.
Wherein, effective viable bacteria total content 6.0 × 10 in the composite bacterial fertilizer9cfu/g.Wherein contain bacillus subtilis
2.5×109Cfu/g, bacillusmusilaginosiengineering 1.5 × 109Cfu/g and bacillus amyloliquefaciens 2.0 × 109cfu/g。
Microbial compound bacterial fertilizer preparation method is the same as embodiment 1
Embodiment 3
Application of the microbial inoculum on fruit crop made of embodiment 1
Test site：Nanjing six directions high and new technology industrial development zone arable farming base
Test period：In March, 2015 in October, 2015
Test crop：Tomato
This product amount of application:15kg/ha
Processing method：This product 15kg/ha is mixed with common fertilizer and is manured into soil as base manure
Experiment is for examination soil main physical and chemical such as table 1
Table 1 is for examination soil main physical and chemical
Using the influence after this product to tomato biological character
Influence after the administration this product of table 2 to tomato biological character
Result of the test shows, this product is mixed with common fertilizer after being manured into soil, plant growth is vigorous, and plant is good for
Strong, leaf dark green, plant height and single fruit weight increase by 7.02%, 8% respectively；Pest and disease damage incidence reduces by 7.5%.Experiment shows this production
Product are obvious as auxiliary fertile effect of increasing production, can effectively strengthen resistivity of the tomato to pest and disease damage, improve Tomato Quality.
Embodiment 4
Application of the microbial inoculum on vegetable crop made of embodiment 1
Test site：Nanjing six directions high and new technology industrial development zone arable farming base
Test period：In April, 2015 in December, 2015
Test crop：Romaine lettuce
This product amount of application:15kg/ha
Processing method：This product 15kg/ha is mixed with common fertilizer and is manured into soil as base manure
Experiment is for examination soil main physical and chemical such as table 3
Table 3 is for examination soil main physical and chemical
Using such as table 4 of the influence after this product to romaine lettuce biological character
Influence after the administration this product of table 4 to romaine lettuce biological character
Result of the test shows, this product is mixed with common fertilizer after being manured into soil, plant growth is vigorous, and blade is dense
Green, plant height and root long increase by 11.6%, 13.9% respectively；Romaine lettuce fresh weight increase by 11.28%；Pest and disease damage incidence reduces by 8.3%,
It is right.Experiment shows that this product can remarkably promote the growth of romaine lettuce rhizome as auxiliary fertilizer, obvious to romaine lettuce effect of increasing production, while to disease
Bacterium has obvious inhibitory action, improves the quality of romaine lettuce.
Embodiment 5
As application of the base manure on cereal crops
Test site：Nanjing six directions high and new technology industrial development zone arable farming base
Test period：In May, 2015 in October, 2015
Test crop：Corn (Zheng Dan 958)
This product amount of application：80kg/ha.
Routine Management：Full nitrogen 10kg/ha, P2O57.5kg/ha, K2O 7.5kg/ha。
Experiment is for examination soil main physical and chemical such as table 1
Table 1 is for examination soil main physical and chemical
Table 2 changes after applying this product for examination soil main physical and chemical and crop yield
Result of the test shows, using this product as base manure after, soil organic matter content dramatically increases, n p k nutrition member
Element also a large amount of accumulation, while salt content and pH value are greatly lowered in soil.As a result prove, this product can effectively improved soil
Structure, increase soil fertility, keep soil moisture and nutrition, reduce soil pH value and salt content.After this product, make produce
Amount dramatically increases, than Routine Management volume increase 10.9%.
Claims (10)
1. a kind of microbial compound bacterial fertilizer, it is characterised in that it includes the component of following parts by weight：
2. microbial compound bacterial fertilizer according to claim 1, it is characterised in that described complex microorganism includes withered grass bud
Spore bacillus, bacillusmusilaginosiengineering and bacillus amyloliquefaciens；Wherein, the deposit number of bacillus subtilis is CCTCC M
2016264, the deposit number of bacillusmusilaginosiengineering is CCTCC M 2016265, and the deposit number of bacillus amyloliquefaciens is
CCTCC M 2016266。
3. microbial compound bacterial fertilizer according to claim 2, it is characterised in that in complex microorganism, bacillus subtilis
The total plate count of bacterium is 2.0 × 109~7.0 × 109Cfu/g, the total plate count of bacillusmusilaginosiengineering is 1.5 × 109~5.0
×109Cfu/g, the total plate count of bacillus amyloliquefaciens is 0.5 × 109~2.5 × 109cfu/g。
4. microbial compound bacterial fertilizer according to claim 1, it is characterised in that described compound nutritional carrier is by mass ratio
3~4：1~3：2~3：1~2：0.5~2：0.5~1 edible fungus culturing waste material, brid guano, sawdust, stalk, humic acid and konjaku
Powder is mixed to get after drying and crushing is sieved.
5. microbial compound bacterial fertilizer according to claim 1, it is characterised in that described EDTA- metallo-chelates by
EDTA-Fe, EDTA-Cu, EDTA-Zn, EDTA-Mn and EDTA-Mg are with 2~3：1~2：3~4：0.5~2：0.5~2 quality
Than mixing.
6. microbial compound bacterial fertilizer according to claim 1, it is characterised in that described diatomite crosses 200~400 mesh
Sieve；Wherein, in diatomite, SiO2Mass percent be 70~75%.
7. the preparation method of the microbial compound bacterial fertilizer described in claim 1, it is characterised in that it comprises the following steps：
(1) by Bacillus globigii spores powder, bacillusmusilaginosiengineering conidia powder and bacillus amyloliquefaciens conidia powder by formula
After amount mixing, complex microorganism is obtained；
(2) mixed after edible fungus culturing waste material, brid guano, sawdust, stalk, humic acid and konjaku flour drying and crushing being sieved by formula ratio
Close uniformly, compound nutritional carrier is obtained after sterilizing；
(3) obtained after being well mixed EDTA-Fe, EDTA-Cu, EDTA-Zn, EDTA-Mn and EDTA-Mg according to formula ratio
EDTA- metallo-chelates are standby；
(4) by the compound nutritional carrier being prepared in the complex microorganism being prepared in step (1), step (2), step
(3) after the diatomite after the EDTA- metallo-chelates being prepared in and sieving is well mixed by formula ratio, produces microorganism and answer
Close bacterial manure.
8. application of the microbial compound bacterial fertilizer in soil improvement and promotion crop growth described in claim 1.
9. application according to claim 8, it is characterised in that application process is：Microorganism described in claim 1 is answered
Bacterial manure is closed as crop Special basal fertilizer, dosage is 50~100kg/ mus.
10. application according to claim 8, it is characterised in that application process is：By the microorganism described in claim 1
Composite bacterial fertilizer aids in base manure as crop, and fiting chemical Fertilizer application, dosage is 10~15kg/ mus.
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Cited By (6)
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| CN108794187A (en) * | 2018-07-24 | 2018-11-13 | 南京轩凯生物科技有限公司 | A kind of microbial liquid microbial inoculum and its preparation method and application |
| CN108911843A (en) * | 2018-07-26 | 2018-11-30 | 山东土秀才生物科技有限公司 | A kind of biological organic fertilizer rich in humic acid |
| CN109294941A (en) * | 2018-09-10 | 2019-02-01 | 天津大学 | A kind of microbial compound bacterial fertilizer and the application in promotion plant absorption N P and K |
| CN110317754A (en) * | 2019-06-28 | 2019-10-11 | 轩凯生物科技(滁州)有限公司 | A kind of microbial liquid microbial inoculum and preparation method thereof containing polyglutamic acid |
| CN114133304A (en) * | 2021-12-21 | 2022-03-04 | 微生物肥料技术研究推广中心 | Water-retention and fertilizer-retention composite microbial fertilizer and preparation method thereof |
| CN115322049A (en) * | 2022-09-21 | 2022-11-11 | 井冈山大学 | Mineral biological compound bacterial fertilizer and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02135026A (en) * | 1988-11-14 | 1990-05-23 | Yasuo Akahori | New culture soil for growing tea and analog plant |
| CN105198672A (en) * | 2015-09-28 | 2015-12-30 | 湖南泰谷生物科技股份有限公司 | Phosphorus- and potassium-solubilizing microorganism fertilizer and preparation method and application thereof |
| CN105237110A (en) * | 2015-08-27 | 2016-01-13 | 青岛深蓝肥业有限公司 | Anti-disease composite microbial fertilizer specially-used for American ginseng and preparation method thereof |
| CN105565970A (en) * | 2015-12-15 | 2016-05-11 | 赤峰蒙鼎生物科技有限公司 | Novel multifunctional compound bio-fertilizer and preparation method thereof |
| CN105585379A (en) * | 2015-12-21 | 2016-05-18 | 云南省微生物发酵工程研究中心有限公司 | Biological compound fertilizer and application thereof |
-
2016
- 2016-08-12 CN CN201610665832.4A patent/CN107721713A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02135026A (en) * | 1988-11-14 | 1990-05-23 | Yasuo Akahori | New culture soil for growing tea and analog plant |
| CN105237110A (en) * | 2015-08-27 | 2016-01-13 | 青岛深蓝肥业有限公司 | Anti-disease composite microbial fertilizer specially-used for American ginseng and preparation method thereof |
| CN105198672A (en) * | 2015-09-28 | 2015-12-30 | 湖南泰谷生物科技股份有限公司 | Phosphorus- and potassium-solubilizing microorganism fertilizer and preparation method and application thereof |
| CN105565970A (en) * | 2015-12-15 | 2016-05-11 | 赤峰蒙鼎生物科技有限公司 | Novel multifunctional compound bio-fertilizer and preparation method thereof |
| CN105585379A (en) * | 2015-12-21 | 2016-05-18 | 云南省微生物发酵工程研究中心有限公司 | Biological compound fertilizer and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 王正银: "《肥料研制与加工 第2版》", 31 December 2015 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108794187A (en) * | 2018-07-24 | 2018-11-13 | 南京轩凯生物科技有限公司 | A kind of microbial liquid microbial inoculum and its preparation method and application |
| CN108911843A (en) * | 2018-07-26 | 2018-11-30 | 山东土秀才生物科技有限公司 | A kind of biological organic fertilizer rich in humic acid |
| CN109294941A (en) * | 2018-09-10 | 2019-02-01 | 天津大学 | A kind of microbial compound bacterial fertilizer and the application in promotion plant absorption N P and K |
| CN110317754A (en) * | 2019-06-28 | 2019-10-11 | 轩凯生物科技(滁州)有限公司 | A kind of microbial liquid microbial inoculum and preparation method thereof containing polyglutamic acid |
| CN114133304A (en) * | 2021-12-21 | 2022-03-04 | 微生物肥料技术研究推广中心 | Water-retention and fertilizer-retention composite microbial fertilizer and preparation method thereof |
| CN115322049A (en) * | 2022-09-21 | 2022-11-11 | 井冈山大学 | Mineral biological compound bacterial fertilizer and preparation method thereof |
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