CN105198672A - Phosphorus- and potassium-solubilizing microorganism fertilizer and preparation method and application thereof - Google Patents
Phosphorus- and potassium-solubilizing microorganism fertilizer and preparation method and application thereof Download PDFInfo
- Publication number
- CN105198672A CN105198672A CN201510626796.6A CN201510626796A CN105198672A CN 105198672 A CN105198672 A CN 105198672A CN 201510626796 A CN201510626796 A CN 201510626796A CN 105198672 A CN105198672 A CN 105198672A
- Authority
- CN
- China
- Prior art keywords
- fermentation
- microorganism fertilizer
- cake
- aspergillus niger
- parts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000003337 fertilizer Substances 0.000 title claims abstract description 75
- 244000005700 microbiome Species 0.000 title claims abstract description 55
- 238000002360 preparation method Methods 0.000 title claims description 34
- 238000000855 fermentation Methods 0.000 claims abstract description 70
- 230000004151 fermentation Effects 0.000 claims abstract description 70
- 229910052700 potassium Inorganic materials 0.000 claims abstract description 66
- 229910052698 phosphorus Inorganic materials 0.000 claims abstract description 61
- 239000000843 powder Substances 0.000 claims abstract description 43
- 241000228245 Aspergillus niger Species 0.000 claims abstract description 35
- 239000002689 soil Substances 0.000 claims abstract description 35
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims abstract description 32
- 239000011591 potassium Substances 0.000 claims abstract description 32
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims abstract description 28
- 239000011574 phosphorus Substances 0.000 claims abstract description 28
- 241000194107 Bacillus megaterium Species 0.000 claims abstract description 17
- 230000000813 microbial effect Effects 0.000 claims abstract description 17
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 claims abstract description 16
- 238000001035 drying Methods 0.000 claims abstract description 14
- 240000002791 Brassica napus Species 0.000 claims abstract description 9
- 235000004977 Brassica sinapistrum Nutrition 0.000 claims abstract description 9
- 238000012216 screening Methods 0.000 claims abstract description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 43
- 239000002131 composite material Substances 0.000 claims description 31
- 241000233866 Fungi Species 0.000 claims description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 23
- 238000002156 mixing Methods 0.000 claims description 16
- 210000003608 fece Anatomy 0.000 claims description 13
- 239000010871 livestock manure Substances 0.000 claims description 13
- 239000007787 solid Substances 0.000 claims description 13
- 239000000725 suspension Substances 0.000 claims description 12
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 9
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 9
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 9
- 239000000463 material Substances 0.000 claims description 7
- 241000194108 Bacillus licheniformis Species 0.000 claims description 5
- 241000223259 Trichoderma Species 0.000 claims description 5
- 239000004546 suspension concentrate Substances 0.000 claims description 4
- 229910004298 SiO 2 Inorganic materials 0.000 claims description 3
- 239000011248 coating agent Substances 0.000 claims description 3
- 238000000576 coating method Methods 0.000 claims description 3
- 235000012054 meals Nutrition 0.000 claims description 3
- 230000003020 moisturizing effect Effects 0.000 claims description 3
- 239000012141 concentrate Substances 0.000 claims description 2
- 230000001131 transforming effect Effects 0.000 claims description 2
- 239000000126 substance Substances 0.000 abstract description 5
- 244000063299 Bacillus subtilis Species 0.000 abstract description 4
- 235000014469 Bacillus subtilis Nutrition 0.000 abstract description 4
- 241000881860 Paenibacillus mucilaginosus Species 0.000 abstract description 4
- 230000035558 fertility Effects 0.000 abstract description 4
- 238000010521 absorption reaction Methods 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 244000000231 Sesamum indicum Species 0.000 abstract description 2
- 235000003434 Sesamum indicum Nutrition 0.000 abstract description 2
- 239000002054 inoculum Substances 0.000 abstract 3
- 235000019764 Soybean Meal Nutrition 0.000 abstract 1
- 239000007633 bacillus mucilaginosus Substances 0.000 abstract 1
- 229910000278 bentonite Inorganic materials 0.000 abstract 1
- 239000000440 bentonite Substances 0.000 abstract 1
- 238000000354 decomposition reaction Methods 0.000 abstract 1
- 235000016709 nutrition Nutrition 0.000 abstract 1
- 230000035764 nutrition Effects 0.000 abstract 1
- 239000004455 soybean meal Substances 0.000 abstract 1
- 239000002609 medium Substances 0.000 description 18
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 16
- 235000002566 Capsicum Nutrition 0.000 description 14
- 238000012360 testing method Methods 0.000 description 12
- 241000894006 Bacteria Species 0.000 description 11
- 230000001580 bacterial effect Effects 0.000 description 11
- 239000007788 liquid Substances 0.000 description 10
- 238000000034 method Methods 0.000 description 10
- 240000008574 Capsicum frutescens Species 0.000 description 9
- 229910019142 PO4 Inorganic materials 0.000 description 9
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 9
- 239000010452 phosphate Substances 0.000 description 9
- 239000001390 capsicum minimum Substances 0.000 description 8
- 238000011218 seed culture Methods 0.000 description 8
- 150000001875 compounds Chemical class 0.000 description 7
- 239000001963 growth medium Substances 0.000 description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 6
- 244000068988 Glycine max Species 0.000 description 6
- 235000010469 Glycine max Nutrition 0.000 description 6
- 239000006002 Pepper Substances 0.000 description 6
- 241000722363 Piper Species 0.000 description 6
- 235000016761 Piper aduncum Nutrition 0.000 description 6
- 235000017804 Piper guineense Nutrition 0.000 description 6
- 235000008184 Piper nigrum Nutrition 0.000 description 6
- 229930006000 Sucrose Natural products 0.000 description 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 6
- 229940041514 candida albicans extract Drugs 0.000 description 6
- 239000008103 glucose Substances 0.000 description 6
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 6
- 235000019341 magnesium sulphate Nutrition 0.000 description 6
- 210000000582 semen Anatomy 0.000 description 6
- 239000005720 sucrose Substances 0.000 description 6
- 239000012138 yeast extract Substances 0.000 description 6
- 230000008569 process Effects 0.000 description 5
- 239000002994 raw material Substances 0.000 description 5
- GNFTZDOKVXKIBK-UHFFFAOYSA-N 3-(2-methoxyethoxy)benzohydrazide Chemical compound COCCOC1=CC=CC(C(=O)NN)=C1 GNFTZDOKVXKIBK-UHFFFAOYSA-N 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- FGUUSXIOTUKUDN-IBGZPJMESA-N C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 Chemical compound C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 FGUUSXIOTUKUDN-IBGZPJMESA-N 0.000 description 4
- 239000001888 Peptone Substances 0.000 description 4
- 108010080698 Peptones Proteins 0.000 description 4
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 4
- 229920002472 Starch Polymers 0.000 description 4
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- 239000013530 defoamer Substances 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 229910052618 mica group Inorganic materials 0.000 description 4
- 235000019319 peptone Nutrition 0.000 description 4
- 239000011343 solid material Substances 0.000 description 4
- 239000008107 starch Substances 0.000 description 4
- 235000019698 starch Nutrition 0.000 description 4
- 238000009423 ventilation Methods 0.000 description 4
- 241000723346 Cinnamomum camphora Species 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 229910052500 inorganic mineral Inorganic materials 0.000 description 3
- 239000010445 mica Substances 0.000 description 3
- 235000010755 mineral Nutrition 0.000 description 3
- 239000011707 mineral Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 230000000050 nutritive effect Effects 0.000 description 3
- 239000004576 sand Substances 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 2
- 235000002568 Capsicum frutescens Nutrition 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 241000726221 Gemma Species 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 230000001186 cumulative effect Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 2
- 230000002538 fungal effect Effects 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 239000002686 phosphate fertilizer Substances 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 235000009566 rice Nutrition 0.000 description 2
- 239000011435 rock Substances 0.000 description 2
- 235000015099 wheat brans Nutrition 0.000 description 2
- 208000019025 Hypokalemia Diseases 0.000 description 1
- 235000019779 Rapeseed Meal Nutrition 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 229940058344 antitrematodals organophosphorous compound Drugs 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 239000002361 compost Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000005059 dormancy Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 239000010433 feldspar Substances 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 238000009533 lab test Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 235000019691 monocalcium phosphate Nutrition 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 150000002903 organophosphorus compounds Chemical class 0.000 description 1
- 208000007645 potassium deficiency Diseases 0.000 description 1
- 229910001577 potassium mineral Inorganic materials 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 239000004456 rapeseed meal Substances 0.000 description 1
- 238000002407 reforming Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000003381 solubilizing effect Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- GOLXNESZZPUPJE-UHFFFAOYSA-N spiromesifen Chemical compound CC1=CC(C)=CC(C)=C1C(C(O1)=O)=C(OC(=O)CC(C)(C)C)C11CCCC1 GOLXNESZZPUPJE-UHFFFAOYSA-N 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- 229910000391 tricalcium phosphate Inorganic materials 0.000 description 1
- 235000019731 tricalcium phosphate Nutrition 0.000 description 1
- 229940078499 tricalcium phosphate Drugs 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides phosphorus- and potassium-solubilizing microorganism fertilizer. The phosphorus- and potassium-solubilizing microorganism fertilizer is composed of, by weight, 5-25 parts of complex inoculant, 65-90 parts of carrier and 5-10 parts of bentonite. The complex inoculant contains Bacillus subtilis, Bacillus megatherium, Bacillus mucilaginosus and Aspergillus niger, and the carrier is powder formed by rapeseed cake, soybean meal cake and sesame cake through fermentation and decomposition through microbial inoculant for fermentation and drying and screening. Slightly soluble or insoluble phosphorus and potassium substances in soil can be converted into quick-action phosphorus and potassium which can be absorbed and utilized by crops, absorption, to other nutrition elements, of the crops is promoted, crop yield is increased, crop quality is improved, environment pollution is reduced, physiochemical property of soil is improved, soil fertility is improved, and production cost is lowered.
Description
Technical field
The present invention relates to biological fertilizer field, specifically, relate to microorganism fertilizer of a kind of P and K decomposing and preparation method thereof and application.
Background technology
Although China's Soil Phosphorus, potassium mineral standing stock are very abundant, per kilogram soil is about containing full phosphorus 400 ~ 200mg, in 20cm arable layer, every mu about contains 1500 ~ 4500 kilograms, potassium, but the phosphorus that crop can directly utilize only has wherein 5%, and the potassium overwhelming majority is present in feldspar, mica group primary mineral, directly can not be utilized by crop.At present, China has the arable land of 74% to lack phosphorus, the arable land potassium deficiency of about 60%.In soil, the phosphorus of 95% is invalid form, and crop is difficult to directly absorb, and wherein insoluble organophosphorus accounts for 20% ~ 50% of total Phosphorus In Soil, accounts for 10% ~ 85% of insoluble soil phosphorus total amount.The phosphate fertilizer applied utilising efficiency in this is 5% ~ 25% in season, and major part and phosphate fertilizer accumulate in soil as invalid state or indissoluble state.Equally, can not be accounted for 95% of general potassium amount by the mineral K be present in potassium felspar sand, mica of crop utilization, the available potassium of energy utilization absorbed by crops only accounts for 2% of potassium total amount.Meanwhile, soil effective K content continuous decrease, causes Nutrient Elements in Soil out of proportion, has had a strong impact on agriculture Sustainable development.
Long-term application of P fertilizer, the phosphorus storehouse content making most of agricultural land soil potential increases, and provides the phosphorus of crop growth very little, and soil lacks phosphorus and is " genetics lacks phosphorus " but not " soil science lacks phosphorus ".How to excavate Potential soil phosphorus storehouse, potassium base resource, reduce that chemical fertilizer is excessive uses the environmental pollution caused, development green agriculture is produced, and becomes a hot issue of current agricultural research.
The exploitation of microorganisms with specific functions or multifunctional microbial group, facilitates the development of microbial fertilizer industry.In national achievement key popularization plan, in the nineties, is listed microbial fertilizer as agricultural high-tech product by China.Phosphate solubilizing microorganism is dissolved insoluble in soil or insoluble phosphorus element, and silicate-dissolving microbe can dissolve potassium felspar sand, mica, phosphatic rock, makes potassium element free out, absorbs for crop.Therefore, the microbial fertilizer utilizing P and K decomposing microorganism to make has great development and application values.
Summary of the invention
In order to solve problems of the prior art, the object of this invention is to provide microorganism fertilizer of a kind of P and K decomposing and preparation method thereof and application.
In order to realize the object of the invention, first the present invention provides a kind of microorganism fertilizer of P and K decomposing, and it is made up of the component of following weight part: composite fungus agent 5 ~ 25 parts, 65 ~ 90 parts, carrier, wilkinite 5 ~ 10 parts;
Wherein, subtilis (Bacillussubtilis), bacillus megaterium (Bacillusmegaterium), bacillusmusilaginosiengineering (Paenibacillusmucilaginosus) and aspergillus niger (Aspergillusniger) is contained in described composite fungus agent; Described carrier is rapeseed cake, dregs of beans cake and sesame-send cake by fermentation with after microbiobacterial agent fermentation, becoming thoroughly decomposed, the meal after drying and screening.
As preferably, it is made up of the component of following weight part: composite fungus agent 10 ~ 20 parts, 75 ~ 90 parts, carrier, wilkinite 5 ~ 8 parts form;
More preferred, it is made up of the component of following weight part: composite fungus agent 15 parts, 80 parts, carrier, wilkinite 5 parts form.
Further, subtilis (Bacillussubtilis) 2.0 ~ 8.0 hundred million cfu/g, bacillus megaterium (Bacillusmegaterium) 1.5 ~ 6.0 hundred million cfu/g, bacillusmusilaginosiengineering (Paenibacillusmucilaginosus) 1.5 ~ 6.0 hundred million cfu/g and aspergillus niger (Aspergillusniger) 0.5 ~ 2.0 hundred million cfu/g is contained in described composite fungus agent;
As preferably, containing subtilis 4.5 hundred million cfu/g, bacillus megaterium 3.5 hundred million cfu/g, bacillusmusilaginosiengineering 3.5 hundred million cfu/g and aspergillus niger 0.5 hundred million cfu/g in described composite fungus agent.
As preferably, described carrier by rapeseed cake, dregs of beans cake, sesame-send cake and fermentation microbiobacterial agent by 5: 3: 2: 0.1 weight ratio mix, ferment.
This ratio can be fermented faster, decomposes the protein ingredient of dregs of rice class material simultaneously, discharges total free aminoacids, improves crop quality.
As preferably, described fermentation microbiobacterial agent comprises subtilis, yeast saccharomyces cerevisiae, trichoderma harziarum and Bacillus licheniformis, and the ratio of the viable count of above-mentioned four kinds of microorganisms in fermentation microbiobacterial agent is 4:1:0.5:4.This ratio is conducive to carrying out fast of compost.
As preferably, described wilkinite crosses 300 mesh sieves, wherein SiO
2content is 70%.
300 object fineness, 70%SiO
2content makes microorganism can not go to utilize the phosphorus in fertilizer and potassium, can better dormancy preserve.
Further, described subtilis (Bacillussubtilis), bacillus megaterium (Bacillusmegaterium), bacillusmusilaginosiengineering (Paenibacillusmucilaginosus) and aspergillus niger (Aspergillusniger) are Chinese agriculture Microbiological Culture Collection administrative center (AgriculturalCultureCollectionofChina english abbreviation ACCC) preservation of bacteria strain.The deposit number of described subtilis is ACCC10118, the deposit number of bacillus megaterium is ACCC10010, the deposit number of bacillusmusilaginosiengineering is ACCC10012, the deposit number of aspergillus niger is ACCC30785.
Further, described microorganism fertilizer is pulvis.
Invention further provides the preparation method of described microorganism fertilizer, comprise the following steps:
(1) preparation of composite fungus agent
I. the preparation of microbial spores powder: after respectively subtilis, bacillus megaterium, bacillusmusilaginosiengineering original strain aseptically being carried out successively slant culture, shaking table cultivation, fermentor cultivation, the fermentation liquor obtained is crossed concentrate drying and is prepared into microbial spores powder;
II. the preparation of aspergillus niger spore powder: after aspergillus niger original strain aseptically being carried out successively slant culture, shaking table cultivation, fermentor cultivation, solid fermentation product spore, the substratum producing spore completely obtained is immersed in clear water respectively and makes spore suspension, thus obtain aspergillus niger spore suspension, then the aspergillus niger spore suspension concentrate drying obtained is prepared into aspergillus niger spore powder;
III. by above-mentioned obtained microbial spores powder, the mixing of aspergillus niger spore powder, composite fungus agent is obtained;
(2) preparation of carrier
Rapeseed cake, dregs of beans cake, sesame-send cake and fermentation are pressed the weight ratio mixing of 5: 3: 2: 0.1 with microbiobacterial agent, regulate moisture to 50% ~ 60%, pile high 1.0 ~ 1.5 meters, stack retting 15 ~ 20 days;
Coating film when fermenting initial, removes film when fermentation heap core temperature is more than 50 DEG C, uses stack turner turning when fermentation heap core temperature is more than 65 DEG C, and suitable moisturizing in first 10 days of fermenting, makes fermentation heap moisture maintain 50% ~ 60%; Repeatable operation like this, treat that fermentation heap color becomes coffee-like or black, fermentation heap temperature is down to 40 DEG C simultaneously, and water-content terminates fermentation below 30%, by the cake fertilizer fermented drying, pulverizes, sieves, obtain carrier; Wherein, subtilis 400,000,000 cfu/g, yeast saccharomyces cerevisiae 100,000,000 cfu/g, trichoderma harziarum 0.5cfu hundred million/g and Bacillus licheniformis 400,000,000 cfu/g is contained in fermentation microbiobacterial agent;
(3) preparation of P and K decomposing microorganism fertilizer: take composite fungus agent, carrier, wilkinite in proportion, namely obtains P and K decomposing microorganism fertilizer after mixing.
Further, in step (1), I, II are specially:
I. the preparation of microbial spores powder:
A. slant culture: subtilis, bacillus megaterium, bacillusmusilaginosiengineering original strain are aseptically inoculated on slant medium respectively, cultivates 24 ~ 36 hours under 30 ± 2 DEG C of conditions;
B. shaking table is cultivated: be aseptically inoculated in respectively in seed culture medium by the bacterial classification that step a cultivates, and under pH7.0 ~ 7.5,30 DEG C of conditions, 160 ~ 180r/min shaking table is cultivated 12 ~ 18 hours;
C. fermentor cultivation: the bacterial classification that step b cultivates aseptically is inoculated in liquid fermentation medium respectively, in pH7.0 ~ 8.0, tank pressure 0.5kg, 30 DEG C, under the condition of ventilation 1:0.8 ~ 1.1, cultivate after 48 ~ 72 hours, viable count is greater than 1.0 × 10
9cfu/mL, 80% thalline becomes gemma tank at present, obtains fermented liquid;
D., after the fermentation liquor obtained in step c being concentrated, each microbial spores powder is prepared into by suitable proportion and carrier chaff powder combination drying;
Wherein, the formula of the slant medium used in step a is as follows: glucose 15g, peptone 5g, yeast extract paste 5g, agar 15g and water 1000mL; The seed culture based formulas used in step b is as follows: glucose 10g, extractum carnis 5g, yeast powder 5g, starch 10g, soybean cake powder 5g, potassium primary phosphate 0.5g, anhydrous magnesium sulfate 0.2g and water 1000mL; The liquid fermentation medium formula used in step c is as follows: Semen Maydis powder 26kg, soybean cake powder 16kg, ammonium sulfate 4kg, glucose 8kg, yeast powder 2.5kg, peptone 1.7kg, potassium primary phosphate 0.3kg, anhydrous magnesium sulfate 0.12kg and defoamer 50mL, add water to 600L;
II. the preparation of aspergillus niger spore powder:
E. aspergillus niger original strain is aseptically inoculated on slant medium, cultivates 72 hours under 28 DEG C of conditions;
F. shaking table is cultivated: be aseptically inoculated in seed culture medium by the bacterial classification that step e cultivates, under pH6.5 ~ 6.8,30 DEG C of conditions, 150r/min shaking table cultivates 72 hours;
G. fermentor cultivation: the bacterial classification that step f cultivates aseptically is inoculated in liquid fermentation medium respectively, in pH6.5 ~ 6.8, tank pressure 0.5kg, 27 ~ 28 DEG C, ventilation is 1:0.6 ~ 0.8, after 48 hours of incubation, mycelium stops fermentation when accounting for 20% of cumulative volume, carries out solid fermentation and produces spore;
H.. solid fermentation produces spore: be inoculated on solid fermentation substratum by the mycelium after step g by fermentation tank cultivation, cultivate 72 ~ 96 hours, when aspergillus niger 90% produces spore, stop fermentation;
I. the fermentation materials producing spore in step h is completely immersed in clear water and makes spore suspension, thus obtain aspergillus niger spore suspension;
J. the aspergillus niger spore suspension concentrate drying obtained in above-mentioned steps i is made spore powder;
Wherein, the formula of the slant medium used in step e is as follows: sucrose 12g, murphy juice 200g, agar 20g and water 1000mL, pH nature; The seed culture based formulas used in step f is as follows: sucrose 10g, yeast extract paste 0.5g, starch 20g, potassium primary phosphate 0.5g, magnesium sulfate 0.2g, sodium-chlor 0.2g and water 1000mL; The liquid fermentation medium formula used in step g is as follows: soybean cake powder 1.2kg, Semen Maydis powder 3kg, sucrose 9kg, yeast extract paste 0.3kg, magnesium sulfate 0.12kg, sodium-chlor 0.12kg, defoamer 50mL, add water to 600L; Solid fermentation culture medium prescription in step h is as follows: cotton seed hulls, Semen Maydis powder and wheat bran add water by the solid material of 5:2:13 weight ratio mixing composition, then potassium primary phosphate 1.0g/L is added, magnesium sulfate 0.5g/L, obtains solid fermentation substratum, and the weight ratio of solid material and water is 1:0.6.
III. above-mentioned obtained microbial spores powder, fungal spore powder are mixed in proportion, obtain composite fungus agent.
Invention further provides described microorganism fertilizer transforming insoluble in soil or insoluble phosphorus, potassium material be can the rapid available phosphorus of utilization absorbed by crops, application in potassium.
Application process:
1. can be used as Special basal fertilizer, every mu of consumption 50 ~ 100kg; Also can be used as auxiliary base manure, coordinate the base manure of Different Crop to use, every mu of consumption 10 ~ 15kg, can ditch spread, spread manuer in holes, ground spreads fertilizer over the fields;
2. will pour water in time after using this product, fertilizer efficiency is better;
3. timely earthing or arable land is wanted, under not making this product be exposed to sunlight after using this product;
4. forbid with sterilant directly used in combination;
5. shady and cool dry place is deposited in.
Beneficial effect of the present invention is:
The invention provides a kind of microorganism fertilizer of P and K decomposing, it can by insoluble in soil or insoluble phosphorus, potassium material, being converted into can rapid available phosphorus, the potassium of utilization absorbed by crops, promote that crop is to the absorption of other nutritive elements, improve crop yield, improve crop quality, reduce environmental pollution, improve soil physico-chemical property, increase soil fertility, reduce production cost.
P and K decomposing microorganism fertilizer provided by the invention, microbe species collocation rationally, can be decomposed the phosphatic rock in soil, tricalcium phosphate and organo phosphorous compounds, discharge available phosphorus, absorb for crop; Equally, the primary minerals such as this bacterial manure decomposable asymmetric choice net potassium felspar sand, mica, by crop utilization in release soluble potassium to soil.In addition, present invention employs the rapeseed cake after fermentation maturity, dregs of beans cake, sesame-send cake be main carriers, carry out reclaiming and effectively utilizing to waste, good economic benefit can be produced, three cake fertilizer after being become thoroughly decomposed by fermentable can be discharged can for nitrogen, phosphorus, the potassium of Crop, use the nutritive substance that can ensure needed for plant growth as base manure, life-time service can improve soil physico-chemical property preferably.
Microorganism fertilizer production technique provided by the invention is simple, and composite bacteria microbial inoculum miospore number content is high, and the survival time is long, can in soil Fast-propagation, form dominant colony, insoluble phosphorus in soil, potassium changed into phosphorus, the potassium element of the utilization of crop Absorbable rod; Meanwhile, promote that crop is to the absorption of other nutritive elements, promotes plant growth.
The effect of P and K decomposing microorganism fertilizer provided by the invention in field is good.Tested by capsicum field planting, result shows: comparatively control group is good for each economical character of capsicum of use this product; Per mu yield exceeds 17.53% than conventional fertilizer application method.
Embodiment
Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.If do not specialize, the conventional means that technique means used in embodiment is well known to those skilled in the art, is raw materials usedly commercial goods.
Embodiment 1 P and K decomposing microorganism fertilizer and preparation method thereof
P and K decomposing microorganism fertilizer is containing the raw material of following parts by weight:
Composite fungus agent 15 parts;
80 parts, carrier;
Wilkinite 5 parts.
In described composite fungus agent, effective viable bacteria total content is 1,200,000,000 cfu/g, is constructed as follows:
The preparation method of above-mentioned P and K decomposing microorganism fertilizer, comprises the steps:
(1) preparation of composite fungus agent
I. the preparation of microbial spores powder:
A. slant culture: respectively subtilis, bacillus megaterium, bacillusmusilaginosiengineering original strain are aseptically inoculated on slant medium, cultivate 24-36 hour under 30 ± 2 DEG C of conditions;
B. shaking table is cultivated: be aseptically inoculated in respectively in seed culture medium by the bacterial classification that step a cultivates, and under pH7.0-7.5,30 DEG C of conditions, 12-18 hour cultivated by 160-180r/min shaking table;
C. fermentor cultivation: the bacterial classification that step b cultivates aseptically is inoculated in liquid fermentation medium respectively, pH7.0-8.0, tank pressure 0.5kg, 30 DEG C, under ventilation 1:0.8-1.1 condition, cultivate after 48-72 hour, viable count is greater than 1.0 × 10
9cfu/mL, 80% thalline becomes gemma tank at present, obtains fermented liquid;
D., after the fermentation liquor obtained in step c being concentrated, each microbial spores powder is prepared into by suitable proportion and carrier chaff powder combination drying;
Wherein, the formula of the slant medium used in step a is as follows: glucose 15g, peptone 5g, yeast extract paste 5g, agar 15g and water 1000mL; The seed culture based formulas used in step b is as follows: glucose 10g, extractum carnis 5g, yeast powder 5g, starch 10g, soybean cake powder 5g, potassium primary phosphate 0.5g, anhydrous magnesium sulfate 0.2g and water 1000mL; The liquid fermentation medium formula used in step c is as follows: Semen Maydis powder 26kg, soybean cake powder 16kg, ammonium sulfate 4kg, glucose 8kg, yeast powder 2.5kg, peptone 1.7kg, potassium primary phosphate 0.3kg, anhydrous magnesium sulfate 0.12kg and defoamer 50mL, add water to 600L;
II. the preparation of aspergillus niger spore powder:
E. aspergillus niger original strain is aseptically inoculated on slant medium, cultivates 72 hours under 28 DEG C of conditions;
F. shaking table is cultivated: be aseptically inoculated in seed culture medium by the bacterial classification that step e cultivates, under pH6.5 ~ 6.8,30 DEG C of conditions, 150r/min shaking table cultivates 72 hours;
G. fermentor cultivation: the bacterial classification that step f cultivates aseptically is inoculated in liquid fermentation medium respectively, be 1:0.6-0.8 in pH6.5-6.8, tank pressure 0.5kg, 27-28 DEG C, ventilation, after 48 hours of incubation, mycelium stops fermentation when accounting for 20% of cumulative volume, carries out solid fermentation and produces spore;
H.. solid fermentation produces spore: be inoculated on solid fermentation substratum by the mycelium after step g by fermentation tank cultivation, cultivate 72 ~ 96 hours, when aspergillus niger 90% produces spore, stop fermentation;
I. the fermentation materials producing spore in step h is completely immersed in clear water and makes spore suspension, thus obtain aspergillus niger spore suspension;
J. the aspergillus niger spore suspension concentrate drying obtained in above-mentioned steps i is made spore powder;
Wherein, the formula of the slant medium used in step e is as follows: sucrose 12g, murphy juice 200g, agar 20g and water 1000mL, pH nature; The seed culture based formulas used in step f is as follows: sucrose 10g, yeast extract paste 0.5g, starch 20g, potassium primary phosphate 0.5g, magnesium sulfate 0.2g, sodium-chlor 0.2g and water 1000mL; The liquid fermentation medium formula used in step g is as follows: soybean cake powder 1.2kg, Semen Maydis powder 3kg, sucrose 9kg, yeast extract paste 0.3kg, magnesium sulfate 0.12kg, sodium-chlor 0.12kg, defoamer 50mL, add water to 600L; Solid fermentation culture medium prescription in step h is as follows: cotton seed hulls, Semen Maydis powder and wheat bran add water by the solid material of 5:2:13 weight ratio mixing composition, then potassium primary phosphate 1.0g/L is added, magnesium sulfate 0.5g/L, obtains solid fermentation substratum, and the weight ratio of solid material and water is 1:0.6.
III. above-mentioned obtained microbial spores powder, fungal spore powder are mixed in proportion, obtain composite fungus agent.
(2) preparation of carrier
Rapeseed cake, dregs of beans cake, sesame-send cake and fermentation are pressed the weight ratio mixing of 5: 3: 2: 0.1 with microbiobacterial agent, regulate moisture to 60%, pile high 1.2 meters, stack retting 60 days.
Coating film when fermenting initial, removes film when fermentation heap core temperature is more than 50 DEG C, uses stack turner turning when fermentation heap core temperature is more than 65 DEG C, and suitable moisturizing in first 25 days of fermenting, makes fermentation heap moisture maintain 60%; Repeatable operation like this, treat that fermentation heap color becomes coffee-like or black, fermentation heap temperature is down to 40 DEG C simultaneously, and water-content terminates fermentation below 30%, by the cake fertilizer fermented drying, pulverizes, sieves, obtain carrier; Wherein, 0.5cfu hundred million/g and Bacillus licheniformis about 400,000,000 cfu/g is about containing subtilis about 400,000,000 cfu/g, yeast saccharomyces cerevisiae about 100,000,000 cfu/g, trichoderma harziarum in fermentation microbiobacterial agent.
(3) preparation of P and K decomposing microorganism fertilizer: take composite fungus agent, carrier, wilkinite in proportion, namely obtains P and K decomposing microorganism fertilizer after mixing.
Embodiment 2 P and K decomposing microorganism fertilizer and preparation method thereof
P and K decomposing microorganism fertilizer is containing the raw material of following parts by weight:
Composite fungus agent 5 parts;
90 parts, carrier;
Wilkinite 5 parts.
Effective viable bacteria total content Wei≤5.5 hundred million cfu/g in described composite fungus agent, are constructed as follows:
All the other are with embodiment 1.
Embodiment 3 P and K decomposing microorganism fertilizer and preparation method thereof
P and K decomposing microorganism fertilizer is containing the raw material of following parts by weight:
Composite fungus agent 25 parts;
65 parts, carrier;
Wilkinite 10 parts.
Effective viable bacteria total content Wei≤2,200,000,000 cfu/g in described composite fungus agent, are constructed as follows:
All the other are with embodiment 1.
Embodiment 4 P and K decomposing microorganism fertilizer and preparation method thereof
P and K decomposing microorganism fertilizer is containing the raw material of following parts by weight:
Composite fungus agent 10 parts;
85 parts, carrier;
Wilkinite 5 parts.
Effective viable bacteria total content Wei≤5.5 hundred million cfu/g in described composite fungus agent, are constructed as follows:
All the other are with embodiment 1.
The field efficacy of embodiment 5 P and K decomposing microorganism fertilizer
1. test objective:
The unusual effect that checking P and K decomposing microorganism fertilizer promotes chili growth and soil quality.
2. test materials:
Plant: supply examination capsicum variety to be camphor tree port, Xiangyin capsicum.
Fertilizer: the P and K decomposing microorganism fertilizer of embodiment 1,2,3,4, compound fermented rape cake (rapeseed meal+dregs of beans+sesame seed meal fermentation is made, and ratio is 5: 3: 2), Nitrogen, Phosphorus and Potassium, calcium superphosphate.
Test point: in camphor tree port town, Xiangyin County of Hunan Province.Camphor tree town soil plough horizon is deep, physical features is scalariform arrangement, soil is granular structure, physicochemical character is good, and organic content is higher, and soil property is in table 1, warmer climate is moistening, annual frostless season average out to 195 days, be greater than the total effective temperature average out to 3100 DEG C of 10 DEG C, be adapted to very much the growth of capsicum.Preceding crop is paddy rice.
Test period: in by the end of February, 2014 to late July.Start the transplanting carrying out Hot Pepper Seedling by the end of February, early and middle ten days started first batch and gathered May, nearly 45 days of cycle of gathering.
Table 1 is for the main Agrochemical characters of examination soil
3. test design
3.1 experiments divide 3 groups, often organize cultivated area 66.7m
2, each group does 3 parallel laboratory tests.
Experimental group A: the P and K decomposing microorganism fertilizer 1Kg often organizing preparation in Example 1 respectively adds the compound fermented rape cake mixing of 19Kg makees base manure and uses.Other conventional fertilizer applications are normally used by local pepper planting guidance amount.
Experimental group B: the P and K decomposing microorganism fertilizer 1Kg often organizing preparation in Example 2 respectively adds the compound fermented rape cake mixing of 19Kg makees base manure and uses.Other conventional fertilizer applications are normally used by local pepper planting guidance amount
Experimental group C: the P and K decomposing microorganism fertilizer 1Kg often organizing preparation in Example 3 respectively adds the compound fermented rape cake mixing of 19Kg makees base manure and uses.Other conventional fertilizer applications are normally used by local pepper planting guidance amount
Experimental group D: the P and K decomposing microorganism fertilizer 1Kg often organizing preparation in Example 4 respectively adds the compound fermented rape cake mixing of 19Kg makees base manure and uses.Other conventional fertilizer applications are normally used by local pepper planting guidance amount.
Experimental group E: the P and K decomposing microorganism fertilizer 1Kg of often group respectively preparation in Example 1, make base manure use adding the compound fermented rape cake mixing of 19Kg after bacterial manure sterilizing.Other conventional fertilizer applications are normally used by local pepper planting guidance amount.
Experimental group F: often organize and get the compound fermented rape cake of 20Kg respectively and make base manure and use.Other conventional fertilizer applications are normally used by local pepper planting guidance amount.This group is CK group.
Capsicum conventional fertilizer application instructs applied amount in table 2.
Table 2 capsicum conventional fertilizer application instructs applied amount
3.2 tests are implemented:
Perform by 3.1, and carry out field management, record, analysis and the work of meter product.
3.2.1 field management and trial sheet:
The control measures of three every process of group should be consistent, and carry out trial sheet.
A) test site, test period, method design, plot area, community arrangement, multiplicity (adopting the form of chart) is indicated;
B) experimental field landform, the soil texture, soil type, preceding crop kind;
C) fertilization time, method, quantity and number of times etc.;
D) quantity of precipitation of duration of test and irrigation quantity;
E) prevention and control of plant diseases, pest control situation and other farming activities etc.;
F) the upgrowth situation field investigation of crop, comprises surviving rate of transplanting seedlings, growing way, breeding time etc.
3.2.2 gather in the crops and count product
Gradation results (results are carried out according to the guidance of plantation family, locality opportunity), meter produce, and finally add up.
3.2.3 the record such as crop quality, soil fertility and resistance
Record P and K decomposing microorganism fertilizer promotes yield of hot pepper, the lifting of soil fertility.
3.3 effect assessment
3.3.1 output results evaluation
Carry out the process of P and K decomposing microbial-bacterial fertilizer and other respectively process between economical character and volume analysis.Output condition is in table 3.
Table 3 is process economical character and volume analysis table respectively
Appendix A (normative appendix)
Matrix steriling test method
A.1 sample
Random sampling from matrix sample.
A.2 sample survey
A.2.1 medium preparing
Prepare A1, A9, A11, A13 tetra-kinds of substratum in NY/T1114-2006 respectively.
A.2.2 the preparation of bacteria suspension
Take sample 10g (being accurate to 0.01g), add in the sterilized water of the 100mL of band granulated glass sphere, leave standstill 20min, on rotary shaker, 200r/min fully vibrates 30min, makes bacteria suspension.
A.2.3 application of sample and cultivation
Previously prepared four kinds of good solid medium flat boards add 0.1mL bacteria suspension respectively, and with sterile glass scraper, bacteria suspension is applied to culture medium flat plate surface equably, repeat 3 times, under appropriate temperature conditions, cultivate 2d-7d.Blank is made with sterilized water.
A.2.4 sterilising effect qualification
Blank is aseptic to be dropped out now, and total number of bacterial colony≤5 on other culture plate, then this sample can be used as based assays.Otherwise, must sterilizing again.Blank has bacterium colony to occur, steriling test of must reforming.
4. test-results
Test is carried out in strict accordance with testing program, without seedling death phenomenon after transplanting, survives rear growing way and all acts normally.Test-results is as shown in table 3, and test proves that this product effectively can improve capsicum per mu yield value.
Although above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (10)
1. a microorganism fertilizer for P and K decomposing, is characterized in that, it is made up of the component of following weight part: composite fungus agent 5 ~ 25 parts, 65 ~ 90 parts, carrier, wilkinite 5 ~ 10 parts;
Wherein, subtilis, bacillus megaterium, bacillusmusilaginosiengineering and aspergillus niger is contained in described composite fungus agent; Described carrier is rapeseed cake, dregs of beans cake and sesame-send cake by fermentation with after microbiobacterial agent fermentation, becoming thoroughly decomposed, the meal after drying and screening.
2. microorganism fertilizer according to claim 1, is characterized in that, it is made up of the component of following weight part: composite fungus agent 10 ~ 20 parts, 75 ~ 90 parts, carrier, wilkinite 5 ~ 8 parts form;
As preferably, it is made up of the component of following weight part: composite fungus agent 15 parts, 80 parts, carrier, wilkinite 5 parts form.
3. microorganism fertilizer according to claim 1 and 2, it is characterized in that, containing subtilis 2.0 ~ 8.0 hundred million cfu/g, bacillus megaterium 1.5 ~ 6.0 hundred million cfu/g, bacillusmusilaginosiengineering 1.5 ~ 6.0 hundred million cfu/g and aspergillus niger 0.5 ~ 2.0 hundred million cfu/g in described composite fungus agent;
As preferably, containing subtilis 4.5 hundred million cfu/g, bacillus megaterium 3.5 hundred million cfu/g, bacillusmusilaginosiengineering 3.5 hundred million cfu/g and aspergillus niger 0.5 hundred million cfu/g in described composite fungus agent.
4. microorganism fertilizer according to claim 1 and 2, is characterized in that, described carrier, is fermented by the weight ratio mixing of 5: 3: 2: 0.1 by rapeseed cake, dregs of beans cake, sesame-send cake and fermentation microbiobacterial agent.
5. microorganism fertilizer according to claim 4, it is characterized in that, described fermentation microbiobacterial agent comprises subtilis, yeast saccharomyces cerevisiae, trichoderma harziarum and Bacillus licheniformis, and the ratio of the viable count of above-mentioned four kinds of microorganisms in fermentation microbiobacterial agent is 4:1:0.5:4.
6. microorganism fertilizer according to claim 1 and 2, is characterized in that, described wilkinite crosses 300 mesh sieves, wherein SiO
2content is 70%.
7. microorganism fertilizer according to claim 1 and 2, it is characterized in that, the deposit number of described subtilis is ACCC10118, the deposit number of bacillus megaterium is ACCC10010, the deposit number of bacillusmusilaginosiengineering is ACCC10012, the deposit number of aspergillus niger is ACCC30785.
8. the microorganism fertilizer according to any one of claim 1 ~ 7, is characterized in that, described microorganism fertilizer is pulvis.
9. the preparation method of microorganism fertilizer described in any one of claim 1 ~ 8, is characterized in that, comprise the following steps:
(1) preparation of composite fungus agent
I. the preparation of microbial spores powder: after respectively subtilis, bacillus megaterium, bacillusmusilaginosiengineering original strain aseptically being carried out successively slant culture, shaking table cultivation, fermentor cultivation, the fermentation liquor obtained is crossed concentrate drying and is prepared into microbial spores powder;
II. the preparation of aspergillus niger spore powder: after aspergillus niger original strain aseptically being carried out successively slant culture, shaking table cultivation, fermentor cultivation, solid fermentation product spore, the substratum producing spore completely obtained is immersed in clear water respectively and makes spore suspension, thus obtain aspergillus niger spore suspension, then the aspergillus niger spore suspension concentrate drying obtained is prepared into aspergillus niger spore powder;
III. by above-mentioned obtained microbial spores powder, the mixing of aspergillus niger spore powder, composite fungus agent is obtained;
(2) preparation of carrier
Rapeseed cake, dregs of beans cake, sesame-send cake and fermentation are pressed the weight ratio mixing of 5: 3: 2: 0.1 with microbiobacterial agent, regulate moisture to 50% ~ 60%, pile high 1.0 ~ 1.5 meters, stack retting 15 ~ 20 days;
Coating film when fermenting initial, removes film when fermentation heap core temperature is more than 50 DEG C, uses stack turner turning when fermentation heap core temperature is more than 65 DEG C, and suitable moisturizing in first 10 days of fermenting, makes fermentation heap moisture maintain 50% ~ 60%; Repeatable operation like this, treat that fermentation heap color becomes coffee-like or black, fermentation heap temperature is down to 40 DEG C simultaneously, and water-content terminates fermentation below 30%, by the cake fertilizer fermented drying, pulverizes, sieves, obtain carrier; Wherein, subtilis 400,000,000 cfu/g, yeast saccharomyces cerevisiae 100,000,000 cfu/g, trichoderma harziarum 0.5cfu hundred million/g and Bacillus licheniformis 400,000,000 cfu/g is contained in fermentation microbiobacterial agent;
(3) preparation of P and K decomposing microorganism fertilizer: take composite fungus agent, carrier, wilkinite in proportion, namely obtains P and K decomposing microorganism fertilizer after mixing.
10. the microorganism fertilizer described in any one of claim 1 ~ 7 transforming insoluble in soil or insoluble phosphorus, potassium material be can the rapid available phosphorus of utilization absorbed by crops, application in potassium, it is characterized in that, it can be used as Special basal fertilizer, every mu of consumption 50 ~ 100kg; Also can be used as auxiliary base manure, coordinate the base manure of Different Crop to use, every mu of consumption 10 ~ 15kg.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510626796.6A CN105198672A (en) | 2015-09-28 | 2015-09-28 | Phosphorus- and potassium-solubilizing microorganism fertilizer and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510626796.6A CN105198672A (en) | 2015-09-28 | 2015-09-28 | Phosphorus- and potassium-solubilizing microorganism fertilizer and preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105198672A true CN105198672A (en) | 2015-12-30 |
Family
ID=54946683
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510626796.6A Pending CN105198672A (en) | 2015-09-28 | 2015-09-28 | Phosphorus- and potassium-solubilizing microorganism fertilizer and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105198672A (en) |
Cited By (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106399157A (en) * | 2016-08-30 | 2017-02-15 | 上海绿乐生物科技有限公司 | Microbial agent with facility agricultural soil remediation function, and preparation and application of microbial agent |
| CN106670227A (en) * | 2016-12-09 | 2017-05-17 | 天长市天龙泵阀成套设备厂 | Method for synergistically degrading soil organic pollution by combining aqueous extract solution of agaricus bisporus culture waste with microorganisms and plants |
| CN106670226A (en) * | 2016-12-09 | 2017-05-17 | 天长市天龙泵阀成套设备厂 | Biological remediation method for organic pollution of soil |
| CN106811433A (en) * | 2017-02-22 | 2017-06-09 | 广州聚禅现代农业研究院有限公司 | A kind of liquid microbe fertilizer and preparation method with fixing nitrogen, dissolving phosphor and dissolving potassium effect |
| CN107602222A (en) * | 2017-09-21 | 2018-01-19 | 湖南泰谷生物科技股份有限公司 | A kind of tealeaves special fertilizer and preparation method thereof |
| CN107619308A (en) * | 2016-07-13 | 2018-01-23 | 湖南泰谷生物科技股份有限公司 | Prevent and treat root-knot nematode biological organic fertilizer and preparation method and application |
| CN107721713A (en) * | 2016-08-12 | 2018-02-23 | 南京轩凯生物科技有限公司 | A kind of microbial compound bacterial fertilizer and preparation method and application |
| CN108794115A (en) * | 2018-08-14 | 2018-11-13 | 潍坊市信得生物科技有限公司 | A kind of pulvis microbial manure |
| CN108865952A (en) * | 2018-07-24 | 2018-11-23 | 湖南泰谷生态工程有限公司 | A kind of Soluble phosphorus drop cadmium microbial bacterial agent and preparation method thereof |
| CN109797123A (en) * | 2019-03-25 | 2019-05-24 | 湖南泰谷生态工程有限公司 | Microbial bacterial agent and its preparation method and application |
| CN111253948A (en) * | 2020-02-27 | 2020-06-09 | 中国农业科学院农业资源与农业区划研究所 | Method for promoting salt leaching of saline-alkali soil |
| CN112279734A (en) * | 2020-05-18 | 2021-01-29 | 新疆德福优品生物科技有限公司 | Water-retaining biological protein fertilizer suitable for irrigating grey desert soil and preparation method thereof |
| CN112661579A (en) * | 2020-12-24 | 2021-04-16 | 沈阳农业大学 | Phosphate solubilizing microbial preparation capable of improving saline-alkali soil and preparation method thereof |
| CN113372146A (en) * | 2021-05-28 | 2021-09-10 | 淮北师范大学 | Biological organic-inorganic ternary compound fertilizer containing plant growth-promoting bacteria and preparation method thereof |
| CN113929536A (en) * | 2021-11-09 | 2022-01-14 | 贺州戎威农业开发有限责任公司 | Organic fertilizer special for organic tea and capable of improving yield and application method thereof |
| CN114149287A (en) * | 2021-10-27 | 2022-03-08 | 哈尔滨师范大学 | A kind of preparation method and application of solid microbial fertilizer |
| CN114773132A (en) * | 2022-03-18 | 2022-07-22 | 广东华扬环保科技股份有限公司 | A kind of high-efficiency special compound base fertilizer for eucalyptus |
| CN114933501A (en) * | 2022-05-25 | 2022-08-23 | 河北萌帮生物科技有限公司 | Microbial agent granules and preparation method thereof |
| CN114958383A (en) * | 2022-04-26 | 2022-08-30 | 刘佳佩 | Composite soil remediation agent and preparation method thereof |
| CN115536469A (en) * | 2022-10-26 | 2022-12-30 | 四川中烟工业有限责任公司 | Preparation method of special organic fertilizer for cigar core leaves |
| CN118184458A (en) * | 2024-04-08 | 2024-06-14 | 山东金沂蒙生态肥业有限公司 | A nutritious composite bacterial fertilizer for increasing crop yield and a preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103739317A (en) * | 2013-12-30 | 2014-04-23 | 邵素英 | Microecological compound fertilizer |
| CN103773719A (en) * | 2013-12-30 | 2014-05-07 | 邵素英 | Preparation method of microbial compound fertilizer |
| CN103819237A (en) * | 2014-02-07 | 2014-05-28 | 湖南泰谷生物科技股份有限公司 | Microbial fertilizer special for tobaccos, and preparing method thereof |
| CN103819267A (en) * | 2014-02-07 | 2014-05-28 | 湖南泰谷生物科技股份有限公司 | Bacterial manure for peanut planting and preparation method of bacterial manure |
-
2015
- 2015-09-28 CN CN201510626796.6A patent/CN105198672A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103739317A (en) * | 2013-12-30 | 2014-04-23 | 邵素英 | Microecological compound fertilizer |
| CN103773719A (en) * | 2013-12-30 | 2014-05-07 | 邵素英 | Preparation method of microbial compound fertilizer |
| CN103819237A (en) * | 2014-02-07 | 2014-05-28 | 湖南泰谷生物科技股份有限公司 | Microbial fertilizer special for tobaccos, and preparing method thereof |
| CN103819267A (en) * | 2014-02-07 | 2014-05-28 | 湖南泰谷生物科技股份有限公司 | Bacterial manure for peanut planting and preparation method of bacterial manure |
Non-Patent Citations (3)
| Title |
|---|
| 乌栽新，王毓洪，张硕主编: "《生态农业链复合微生物肥料》", 31 October 2012, 中国农业科学技术出版社 * |
| 梁利宝编著: "《微生物肥料与农药在现代农业生产中的应用》", 31 July 2012, 中国农业科学技术出版社 * |
| 陈一定主编: "《新型肥料使用手册》", 31 August 2008, 浙江科学技术出版社 * |
Cited By (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107619308A (en) * | 2016-07-13 | 2018-01-23 | 湖南泰谷生物科技股份有限公司 | Prevent and treat root-knot nematode biological organic fertilizer and preparation method and application |
| CN107721713A (en) * | 2016-08-12 | 2018-02-23 | 南京轩凯生物科技有限公司 | A kind of microbial compound bacterial fertilizer and preparation method and application |
| CN106399157A (en) * | 2016-08-30 | 2017-02-15 | 上海绿乐生物科技有限公司 | Microbial agent with facility agricultural soil remediation function, and preparation and application of microbial agent |
| CN106670227A (en) * | 2016-12-09 | 2017-05-17 | 天长市天龙泵阀成套设备厂 | Method for synergistically degrading soil organic pollution by combining aqueous extract solution of agaricus bisporus culture waste with microorganisms and plants |
| CN106670226A (en) * | 2016-12-09 | 2017-05-17 | 天长市天龙泵阀成套设备厂 | Biological remediation method for organic pollution of soil |
| CN106811433A (en) * | 2017-02-22 | 2017-06-09 | 广州聚禅现代农业研究院有限公司 | A kind of liquid microbe fertilizer and preparation method with fixing nitrogen, dissolving phosphor and dissolving potassium effect |
| CN107602222A (en) * | 2017-09-21 | 2018-01-19 | 湖南泰谷生物科技股份有限公司 | A kind of tealeaves special fertilizer and preparation method thereof |
| CN108865952B (en) * | 2018-07-24 | 2020-12-04 | 湖南泰谷生态工程有限公司 | Phosphorus-dissolving cadmium-reducing microbial agent and preparation method thereof |
| CN108865952A (en) * | 2018-07-24 | 2018-11-23 | 湖南泰谷生态工程有限公司 | A kind of Soluble phosphorus drop cadmium microbial bacterial agent and preparation method thereof |
| CN108794115A (en) * | 2018-08-14 | 2018-11-13 | 潍坊市信得生物科技有限公司 | A kind of pulvis microbial manure |
| CN109797123A (en) * | 2019-03-25 | 2019-05-24 | 湖南泰谷生态工程有限公司 | Microbial bacterial agent and its preparation method and application |
| CN111253948A (en) * | 2020-02-27 | 2020-06-09 | 中国农业科学院农业资源与农业区划研究所 | Method for promoting salt leaching of saline-alkali soil |
| CN112279734A (en) * | 2020-05-18 | 2021-01-29 | 新疆德福优品生物科技有限公司 | Water-retaining biological protein fertilizer suitable for irrigating grey desert soil and preparation method thereof |
| CN112661579A (en) * | 2020-12-24 | 2021-04-16 | 沈阳农业大学 | Phosphate solubilizing microbial preparation capable of improving saline-alkali soil and preparation method thereof |
| CN113372146A (en) * | 2021-05-28 | 2021-09-10 | 淮北师范大学 | Biological organic-inorganic ternary compound fertilizer containing plant growth-promoting bacteria and preparation method thereof |
| CN114149287A (en) * | 2021-10-27 | 2022-03-08 | 哈尔滨师范大学 | A kind of preparation method and application of solid microbial fertilizer |
| CN113929536A (en) * | 2021-11-09 | 2022-01-14 | 贺州戎威农业开发有限责任公司 | Organic fertilizer special for organic tea and capable of improving yield and application method thereof |
| CN114773132A (en) * | 2022-03-18 | 2022-07-22 | 广东华扬环保科技股份有限公司 | A kind of high-efficiency special compound base fertilizer for eucalyptus |
| CN114773132B (en) * | 2022-03-18 | 2022-11-22 | 广东华扬环保科技股份有限公司 | Efficient compound base fertilizer special for eucalyptus |
| CN114958383A (en) * | 2022-04-26 | 2022-08-30 | 刘佳佩 | Composite soil remediation agent and preparation method thereof |
| CN114933501A (en) * | 2022-05-25 | 2022-08-23 | 河北萌帮生物科技有限公司 | Microbial agent granules and preparation method thereof |
| CN115536469A (en) * | 2022-10-26 | 2022-12-30 | 四川中烟工业有限责任公司 | Preparation method of special organic fertilizer for cigar core leaves |
| CN118184458A (en) * | 2024-04-08 | 2024-06-14 | 山东金沂蒙生态肥业有限公司 | A nutritious composite bacterial fertilizer for increasing crop yield and a preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105198672A (en) | Phosphorus- and potassium-solubilizing microorganism fertilizer and preparation method and application thereof | |
| CN103819237B (en) | Tobacco special microorganism is fertile and preparation method thereof | |
| CN103964946B (en) | A kind of paddy rice Special compound microbial fertilizer and preparation method thereof | |
| CN102876576B (en) | Acidic soil conditioner, and preparation method and application thereof | |
| CN102432399B (en) | Biological organic fertilizer for improving stress resistance and output of paddy rice and preparation method thereof | |
| CN103819267B (en) | For the microbial-bacterial fertilizer and preparation method thereof of peanut cultivation | |
| CN103966150B (en) | A kind of agricultural crop straw decomposing microbial inoculum and using method thereof and effect | |
| CN102199049B (en) | Microbial organic manure and preparation method thereof | |
| CN104761379A (en) | Novel multifunctional compound microbial fertilizer and preparation method thereof | |
| CN101717315B (en) | Special peanut organic and inorganic compound fertilizer for biologically preventing and treating root knot nematode disease and preparation method thereof | |
| CN103848698A (en) | Biological organic fertilizer prepared by aerobic fermentation of biogas residue and preparation method thereof | |
| CN104276882A (en) | Combined fertilizer synergist containing polyglutamic acid, phosphate solubilizing bacteria and potassium bacteria and preparation method and application of combined fertilizer synergist | |
| CN103518781B (en) | Core bio-control bacteria and organic fertilizer containing the core bio-control bacteria | |
| CN109652329A (en) | A kind of preparation and application of bacillus solid microbial | |
| CN102584366A (en) | Microbial organic fertilizer as well as preparation method and application thereof | |
| CN106495951A (en) | A kind of high concentration microorganism microbial inoculum containing polyglutamic acid and preparation method and application | |
| CN107619308A (en) | Prevent and treat root-knot nematode biological organic fertilizer and preparation method and application | |
| CN105198509A (en) | Special biological organic fertilizer for cotton and preparation method of special biological organic fertilizer | |
| CN105732136A (en) | Special bio-organic fertilizer for pepper and preparation method thereof | |
| CN106068746B (en) | A kind of salt-soda soil biological modification method | |
| CN104962489A (en) | Liquid straw decomposition agent and preparation and application methods thereof | |
| CN102731213A (en) | Fertilizer for cultivation of tobacco, preparation method and application thereof | |
| CN106242829A (en) | A kind of overcome the soil remediation fertilizer of Radix Salviae Miltiorrhizae continuous cropping obstacle, preparation method and its usage | |
| CN106278654A (en) | A kind of overcome the soil remediation fertilizer of Rhizoma Zingiberis Recens continuous cropping obstacle, preparation method and its usage | |
| CN107285925A (en) | A kind of method of utilization microorganism formulation stalk stack retting returning to the field |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information | ||
| CB02 | Change of applicant information |
Address after: 410000 Hunan Changsha national hi tech Industrial Development Zone, 27 Yuan Lu Yu Yuan Pioneer Building 1506, 1507 Applicant after: Taigu ecological science and technology group Limited by Share Ltd Address before: 410205 A 1403, Lulu business center, No. 199 Lulong Road, Changsha high tech Zone, Hunan Applicant before: Hunan Taigu Biotechnology Co., Ltd. |
|
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20151230 |