CN106670226A - Biological remediation method for organic pollution of soil - Google Patents
Biological remediation method for organic pollution of soil Download PDFInfo
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- CN106670226A CN106670226A CN201611127338.9A CN201611127338A CN106670226A CN 106670226 A CN106670226 A CN 106670226A CN 201611127338 A CN201611127338 A CN 201611127338A CN 106670226 A CN106670226 A CN 106670226A
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- Prior art keywords
- bacterium solution
- soil
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- complex microorganism
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
Abstract
The invention discloses a biological remediation method for organic pollution of soil. The method includes the steps of 1) mixing vegetable waste and crop straw according to a mass ratio being 80-90 to 10-20 and then carrying out stack retting fermentation to obtain fermented and decomposed materials; inoculating the fermented and decomposed materials with composite microbial strains to obtain the propagated composite microbial strains, and mixing the propagated composite microbial strains with corn steep liquor and soybean meal according to the weight ratio being 80-90 to 5-10 to 5-10 to obtain a composite microbial agent; and 2) applying the composite microbial agent to the soil to be restored, wherein the application amount per mu is 100-200 kg, then scattering carrot seeds and sowing the carrot seeds according to a density being 10-15 thousand plants per mu, and after a seed breaking period, spraying mist of the composite microbial strains into the soil according to the usage amount being 100-200ml per mu every 6-8 days until harvest. Due to the synergistic effect of functional microorganisms and plants, the content of organic matter in the soil can be reduced.
Description
Technical field
Present invention relates particularly to a kind of biological renovation method of soil organic contamination.
Background technology
Soil organic contamination is caused by the administration of production activity, farmland Pesticides and insecticide in chemical plant etc., its
In the main pollution for referring to chemical pesticide, first, it can make composition of the soil and physicochemical property change, then destroy soil
The normal composition of earth, can finally pass through the process such as the absorption of plant and the accumulation of food chain, and then constitute danger to health
Evil,.
Spend compared to physics, the large-engineering amount of chemical repair method and high reparation, take bioremediation technology to administer soil
The obvious economical advantage of earth organic contamination, biological restoration includes phytoremediation technology and microorganism remediation technology, and plant is repaiied
Recovering technology has green, energy-conservation, low cost, can directly process etc. in situ advantage, and get more and more people's extensive concerning.It is from original
For in reason, it is divided into 2 classes, a class is plant extract, i.e., organic pollution is absorbed storage in vivo, degraded or by steaming by plant
The effect of rising evaporate into pollutant in air from leaf surfaces, so as to remove or reduce soil pollutant；Two is foundation degraded, i.e.,
Plant roots secrete foundation enzyme, such as digestive enzyme, laccase, remove chlB4, then directly participate in organic pollutant degradation.Microorganism remediation
Being can be by its metabolic activity, by organic pollutant degradation.But microorganism at present and the organic dirt of plant combined application rehabilitating soil
The case of dye is also few, most or adopt single microorganism, is so often affected strongly by the change of environment.
The content of the invention
A kind of biological renovation method of soil organic contamination of offer of the present invention.
In order to solve the above problems, the technical solution adopted in the present invention is a kind of such, life of soil organic contamination
Thing restorative procedure, comprises the steps,
1）By vegetable castoff and crop material 80-90 in mass ratio：After 10-20 mixing, pile fermentation is carried out, it is every to enter within 7-10 days
Turning of row, after fermenting 50-60 days, obtains fermentation maturity material；The inoculating complex microorganism strain in fermentation maturity material,
Inoculum concentration is 2-4% in mass ratio, and daily turning, fermentation 4-5d terminates fermentation, obtains the good complex microorganism bacterial classification of expanding propagation, will expand
Numerous good complex microorganism bacterial classification is with Semen Maydis pulp and bean cake by weight 80-90：5-10：5:After 10 mixing, complex microorganism is obtained
Microbial inoculum；Described complex microorganism bacterial classification is bacillus azotobacter bacterium solution, bacillus megaterium bacterium solution, jelly bacillus cereuss bacterium
Liquid, pseudomonass bacterium solution, arthrobacter bacterium solution and Aspergillus niger spores suspension；
2）Complex micro organism fungicide is applied in soil to be repaired, then every mu of amount of application about 100-200kg is sprinkled into carrot seed
Son, according to the density sowing of every mu of ten thousand plants of 1-1.5, after breaking the tripe phase, every mu of 100- was pressed every 6-8 days by complex microorganism bacterial classification
200mL sprayings are applied in soil until harvesting.
Wherein, the concentration of bacillus azotobacter bacterium solution is 2-3 × 10 8Individual/mL, the concentration of bacillus megaterium bacterium solution is 2
-3×108Individual/mL, the concentration of jelly bacillus cereuss bacterium solution is 2-3 × 108Individual/mL, the concentration of pseudomonass bacterium solution is 3-5
×10 8Individual/mL, the concentration of arthrobacter bacterium solution is 4-5 × 108Individual/mL, the concentration of Aspergillus niger spores suspension is 5-8 × 108
Individual/mL.
Bacillus azotobacter bacterium solution, bacillus megaterium bacterium solution, jelly bacillus cereuss bacterium solution, pseudomonass bacterium solution, section are thin
Bacterium bacterium solution and Aspergillus niger spores suspension 1-3 by volume：1-3：1-3：1-3：1-3：1-3 is mixed into complex microorganism bacterial classification.
Carrot Seed is placed in the warm water of 28-30 degree and soaks 10-12 hours before sowing seeds.
Described Semen Maydis pulp is the by-product of corn starch processed, is that niblet first uses sulfurous acid when corn starch is manufactured
Immersion, gained soak is concentrated to obtain filemot liquid, wherein, containing abundant soluble protein, auxin and some
Precursor substance, can rapidly promote microbial reproduction.
Described vegetable castoff is vegetable melon and fruit vines, old leaf, dead leaf, sick leaf, disease fruit, diseased plant etc..
Beneficial effect：
1st, the function of functional microbial strain of the invention has two aspects, one be can degradation of organic substances, promote soil organic
The degraded of pollutant, improves soil microenvironment, recovers soil fertility, builds up fertility, and increases the tolerance of Radix Dauci Sativae, so as to promote
Make Radix Dauci Sativae fast-growth；Two is have preferable potassium decomposing, degraded soil indissoluble Phos and nitrogen fixation, is the life of Radix Dauci Sativae
It is long that nutrient is provided, quick growth of plant can be made, the absorption storage capacity to organic pollutant is increased, so as to improve phytoremediation
Efficiency.
2nd, the present invention on this basis, with the addition of functional microbial microbial inoculum using fowl and animal excrement and the thing that becomes thoroughly decomposed of distiller grains,
By secondary fermentation so that the viable count of the functional microbial microbial inoculum in final products is increased so as to can be more preferably
Ground adapted soil environment, after being manured into soil, functional microbial is spread rapidly in soil with geometrical progression procreation, with plant association
Same-action, reduces the content of organics in soil.
Specific embodiment
In order to deepen the understanding of the present invention, below in conjunction with embodiment, the invention will be further described, the embodiment
It is only used for explaining the present invention, does not constitute limiting the scope of the present invention.
A kind of biological renovation method of soil organic contamination of embodiment 1, comprises the steps,
1）By vegetable castoff and crop material in mass ratio 80：After 20 mixing, pile fermentation is carried out, it is every to carry out once within 7-10 days
Turning, after fermenting 60 days, obtains fermentation maturity material；The inoculating complex microorganism strain in fermentation maturity material, inoculum concentration is pressed
Mass ratio 2% is carried out, daily turning, and ferment 4-5d, terminates fermentation, obtains the good complex microorganism bacterial classification of expanding propagation, answers expanding propagation is good
Microorganism fungus kind is closed with Semen Maydis pulp and bean cake by weight 80： 10：After 10 mixing, complex micro organism fungicide is obtained；
Wherein, described complex microorganism bacterial classification is bacillus azotobacter bacterium solution（Concentration is 2-3 × 10 8Individual/mL）, huge bud
Spore bacillus bacterium solution（Concentration is 2-3 × 108Individual/mL）, jelly bacillus cereuss bacterium solution（2 -3×108Individual/mL）, pseudomonass bacterium
Liquid（Concentration is 3-5 × 108Individual/mL）, arthrobacter bacterium solution（4-5×108Individual/mL）, and Aspergillus niger spores suspension（Concentration is
5-8 ×108Individual/mL）, bacillus azotobacter bacterium solution, bacillus megaterium bacterium solution, jelly bacillus cereuss bacterium solution, pseudomonass
Bacterium solution, arthrobacter bacterium solution and Aspergillus niger spores suspension by volume 1：1：1：1：1：1 is mixed into complex microorganism bacterial classification.
2）By Nanjing Organic Chemical Plant inner region soil A（Long * width=30*5）After being turned over, complex microorganism is applied
Microbial inoculum, every mu of amount of application about 200kg, is then sprinkled into Carrot Seed（Before sowing seeds, it is placed in the warm water of 28-30 degree and soaks 10
- 12 hours）, sow according to 1.5 ten thousand plants per mu of density, after breaking the tripe phase, complex microorganism bacterial classification is pressed into per mu every 6-8 days
200mL sprayings are applied in soil until harvesting.
Land in Regional Land A is not carried out before biological restoration, and after testing, soil organic pollutant is mainly 4-methyl-2 pentanone,
Toluene, chlorobenzene, ethylbenzene, dimethylbenzene, 1,2- dichloro-benzenes, content is respectively 4.2mg/kg, 1 μ g/ kg, 0.8 μ g/ kg, 0.5 μ g/
Kg, 1.7 μ g/ kg, 2.5 mg/kg；After being processed as described in Example 1,4-methyl-2 pentanone, toluene, chlorobenzene, second
Benzene, dimethylbenzene, the content of 1,2- dichloro-benzenes is respectively 1.8mg/kg, 0.05 μ g/ kg, 0.08 μ g/ kg, 0.02 μ g/ kg,
0.05 μ g/ kg, 0.5 mg/kg.
A kind of biological renovation method of soil organic contamination of embodiment 2, comprises the steps,
1）By vegetable castoff and crop material in mass ratio 80：After 20 mixing, pile fermentation is carried out, it is every to carry out once within 7-10 days
Turning, after fermenting 60 days, obtains fermentation maturity material；The inoculating complex microorganism strain in fermentation maturity material, inoculum concentration is pressed
Mass ratio 2% is carried out, daily turning, and ferment 4-5d, terminates fermentation, obtains the good complex microorganism bacterial classification of expanding propagation, answers expanding propagation is good
Microorganism fungus kind is closed with Semen Maydis pulp and bean cake by weight 80： 10：After 10 mixing, complex micro organism fungicide is obtained；
Wherein, described complex microorganism bacterial classification is bacillus azotobacter bacterium solution（Concentration is 2-3 × 10 8Individual/mL）, huge bud
Spore bacillus bacterium solution（Concentration is 2-3 × 108Individual/mL）, jelly bacillus cereuss bacterium solution（2 -3×108Individual/mL）, pseudomonass bacterium
Liquid（Concentration is 3-5 × 108Individual/mL）, arthrobacter bacterium solution（4-5×108Individual/mL）, and Aspergillus niger spores suspension（Concentration is
5-8 ×108Individual/mL）, bacillus azotobacter bacterium solution, bacillus megaterium bacterium solution, jelly bacillus cereuss bacterium solution, pseudomonass
Bacterium solution, arthrobacter bacterium solution and Aspergillus niger spores suspension by volume 3： 3： 3：1：1：1 is mixed into complex microorganism bacterial classification.
2）By Nanjing Organic Chemical Plant inner region soil B（Long * width=30*5）After being turned over, complex microorganism is applied
Microbial inoculum, every mu of amount of application about 100-200kg, is then sprinkled into Carrot Seed（Before sowing seeds, it is placed in the warm water of 28-30 degree and soaks
Bubble 10-12 hours）, according to the density sowing of every mu of ten thousand plants of 1-1.5, after breaking the tripe phase, every 6-8 days by complex microorganism bacterial classification
It is applied in soil until harvesting by every mu of 100-200mL spraying.
Land in Regional Land B is not carried out before biological restoration, and after testing, soil organic pollutant is mainly 4-methyl-2 pentanone,
Toluene, chlorobenzene, ethylbenzene, dimethylbenzene, 1,2- dichloro-benzenes, content is respectively 4.2mg/kg, 1 μ g/ kg, 0.8 μ g/ kg, 0.5 μ g/
Kg, 1.7 μ g/ kg, 2.2 mg/kg；After being processed as described in Example 1,4-methyl-2 pentanone, toluene, chlorobenzene, second
Benzene, dimethylbenzene, the content of 1,2- dichloro-benzenes is respectively 1.6mg/kg, 0.06 μ g/ kg, 0.08 μ g/ kg, 0.01 μ g/ kg,
0.04 μ g/ kg, 0.5 mg/kg.
A kind of biological renovation method of soil organic contamination of embodiment 3, comprises the steps,
1）By vegetable castoff and crop material in mass ratio 85：After 15 mixing, pile fermentation is carried out, it is every to carry out once within 7-10 days
Turning, after fermenting 60 days, obtains fermentation maturity material；The inoculating complex microorganism strain in fermentation maturity material, inoculum concentration is pressed
Mass ratio 3% is carried out, daily turning, and ferment 4-5d, terminates fermentation, obtains the good complex microorganism bacterial classification of expanding propagation, answers expanding propagation is good
Microorganism fungus kind is closed with Semen Maydis pulp and bean cake by weight 85： 10：After 5 mixing, complex micro organism fungicide is obtained；
Wherein, described complex microorganism bacterial classification is bacillus azotobacter bacterium solution（Concentration is 2-3 × 10 8Individual/mL）, huge bud
Spore bacillus bacterium solution（Concentration is 2-3 × 108Individual/mL）, jelly bacillus cereuss bacterium solution（2 -3×108Individual/mL）, pseudomonass bacterium
Liquid（Concentration is 3-5 × 108Individual/mL）, arthrobacter bacterium solution（4-5×108Individual/mL）, and Aspergillus niger spores suspension（Concentration is
5-8 ×108Individual/mL）, bacillus azotobacter bacterium solution, bacillus megaterium bacterium solution, jelly bacillus cereuss bacterium solution, pseudomonass
Bacterium solution, arthrobacter bacterium solution and Aspergillus niger spores suspension by volume 1：1：1：2：2：2 are mixed into complex microorganism bacterial classification.
2）By Nanjing Organic Chemical Plant inner region soil C（Long * width=30*5）After being turned over, complex microorganism is applied
Microbial inoculum, every mu of amount of application about 150kg, is then sprinkled into Carrot Seed（Before sowing seeds, it is placed in the warm water of 28-30 degree and soaks 10
- 12 hours）, sow according to 1.2 ten thousand plants per mu of density, after breaking the tripe phase, complex microorganism bacterial classification is pressed into per mu every 6-8 days
150mL sprayings are applied in soil until harvesting.
Land in Regional Land C is not carried out before biological restoration, and after testing, soil organic pollutant is mainly 4-methyl-2 pentanone,
Toluene, chlorobenzene, ethylbenzene, dimethylbenzene, 1,2- dichloro-benzenes, content is respectively 4.2mg/kg, 1 μ g/ kg, 0.8 μ g/ kg, 0.5 μ g/
Kg, 1.7 μ g/ kg, 2.5 mg/kg；After being processed as described in Example 1,4-methyl-2 pentanone, toluene, chlorobenzene, second
Benzene, dimethylbenzene, the content of 1,2- dichloro-benzenes is respectively 1.5mg/kg, 0.02 μ g/ kg, 0.05 μ g/ kg, 0.02 μ g/ kg,
0.05 μ g/ kg, 0.4 mg/kg.
In above example, Land in Regional Land A, B, C is adjacent.
Presently preferred embodiments of the present invention is the foregoing is only, not to limit the present invention, all essences in the present invention
Within god and principle, any modification, equivalent substitution and improvements made etc. should be included within the scope of the present invention.
Claims (4)
1. a kind of biological renovation method of soil organic contamination, it is characterised in that comprise the steps,
1）By vegetable castoff and crop material 80-90 in mass ratio：After 10-20 mixing, pile fermentation is carried out, it is every to enter within 7-10 days
Turning of row, after fermenting 50-60 days, obtains fermentation maturity material；The inoculating complex microorganism strain in fermentation maturity material,
Inoculum concentration is 2-4% in mass ratio, and daily turning, fermentation 4-5d terminates fermentation, obtains the good complex microorganism bacterial classification of expanding propagation, will expand
Numerous good complex microorganism bacterial classification is with Semen Maydis pulp and bean cake by weight 80-90：5-10：5:After 10 mixing, complex microorganism is obtained
Microbial inoculum；Described complex microorganism bacterial classification is bacillus azotobacter bacterium solution, bacillus megaterium bacterium solution, jelly bacillus cereuss bacterium
Liquid, pseudomonass bacterium solution, arthrobacter bacterium solution and Aspergillus niger spores suspension；
2）Complex micro organism fungicide is applied in soil to be repaired, then every mu of amount of application about 100-200kg is sprinkled into carrot seed
Son, according to the density sowing of every mu of ten thousand plants of 1-1.5, after breaking the tripe phase, every mu of 100- was pressed every 6-8 days by complex microorganism bacterial classification
200mL sprayings are applied in soil until harvesting.
2. a kind of biological renovation method of soil organic contamination according to claim 1, it is characterised in that fixed nitrogen spore bar
The concentration of bacterium bacterium solution is 2-3 × 10 8Individual/mL, the concentration of bacillus megaterium bacterium solution is 2-3 × 108Individual/mL, jelly spore bar
The concentration of bacterium bacterium solution is 2-3 × 108Individual/mL, the concentration of pseudomonass bacterium solution is 3-5 × 108Individual/mL, arthrobacter bacterium solution
Concentration be 4-5 × 108Individual/mL, the concentration of Aspergillus niger spores suspension is 5-8 × 108Individual/mL.
3. a kind of biological renovation method of soil organic contamination according to claim 2, it is characterised in that fixed nitrogen spore bar
Bacterium bacterium solution, bacillus megaterium bacterium solution, jelly bacillus cereuss bacterium solution, pseudomonass bacterium solution, arthrobacter bacterium solution and Aspergillus niger spores
Suspension 1-3 by volume：1-3：1-3：1-3：1-3：1-3 is mixed into complex microorganism bacterial classification.
4. a kind of biological renovation method of soil organic contamination according to claim 1, it is characterised in that Carrot Seed
Before sowing seeds, it is placed in the warm water of 28-30 degree and soaks 10-12 hours.
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| CN201611127338.9A CN106670226A (en) | 2016-12-09 | 2016-12-09 | Biological remediation method for organic pollution of soil |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109877151A (en) * | 2019-03-08 | 2019-06-14 | 广东宜瑞环境科技有限公司 | A kind of restorative procedure of organic polluted soil |
| CN115254944A (en) * | 2022-06-07 | 2022-11-01 | 福建永强岩土股份有限公司 | Soil remediation method based on application of microorganisms and remediation device thereof |
| CN115338242A (en) * | 2022-08-16 | 2022-11-15 | 生态环境部南京环境科学研究所 | Method for repairing polluted soil by applying wormcast and probiotics in matched manner |
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Application publication date: 20170517 |