RU2437864C1 - Method of microbiological processing of bird droppings - Google Patents

Abstract

FIELD: agriculture.

SUBSTANCE: method includes adding a microbial culture Pseudomonas sp. 114, deposited in VKPM under No. B-5060, into bird droppings, mixing. In 5 days a microbial culture Azotobacter chroococcum B 35 is added, deposited in VKPM under No. B-6010, and again mixed. Titre of added microbial cultures for Pseudomonas sp. makes 114-10⁸ kl/ml, and for Azotobacter chroococcum B 35-10⁸ kl/ml. Volume ratio of added cultures is 2:1 accordingly with 45 ml per 1 kg of bird droppings in case of birds keeping without litters. In case of birds keeping with litters, Pseudomonas sp. 114 and Azotobacter chroococcum B 35, taken as 2:1, are added in amount of 15 ml per 1 kg of droppings. Prior to addition of microbial cultures, each of them is dissolved with water in the ratio of 1:2 accordingly. Microbiological treatment of bird droppings is done for 15 days.

EFFECT: invention helps to accelerate bioconversion process and to simultaneously increase biological activity of the processing product, and to ensure its ecological safety.

4 cl, 11 tbl, 2 ex

Description

The invention relates to agriculture and can be used in the processing of waste poultry farms.

Poultry farms are a significant source of environmental pollution, flies and unpleasant odors spreading over long distances from the litter yard worsen the socio-ecological living and working conditions of poultry workers, as well as the health of animals forced to breathe ammonia vapor and other harmful fumes from settling tanks and prefabricated pits. The problem of recycling poultry farms is also relevant because a large number of arable land is occupied for their storage. Poultry farms are forced to pay heavy fines for environmental violations.

A known method of processing bird droppings, including the processing of bird droppings with mineral acids (RF patent No. 2357944, IPC C05F 3/00 (2006.01)). Fresh bird droppings are mixed with a solution of sulfuric or phosphoric acid, the concentration of a solution of sulfuric or phosphoric acid is from 25% to 35%, while reaching a pH of 5.0 to 6.5. The invention provides a reduction in negative environmental impacts arising from the storage of poultry manure and the production of fertilizer, the reduction of processing time of poultry manure, the possibility of processing a large volume of litter, and the cost of the final product. The disadvantages of the method include its large material consumption, and high concentrations of acids used make it dangerous.

A known method of preparing fertilizers from organic waste of livestock, poultry and crop production (RF patent No. 2371425, IPC (2006.01) C05F 3/00). For this, the biomass is separated into fractions by separation, and disinfection is carried out with the simultaneous detoxification of the liquid fraction in the electrolyzer with insoluble electrodes, and the solid fraction by treatment with an ozone-air mixture and ultraviolet radiation. The technical solution is aimed at increasing the efficiency of the technology for disinfecting and detoxifying manure and poultry manure to ensure sanitary and hygienic working conditions in production facilities and reducing harmful emissions into the environment when preparing organic fertilizers from livestock, poultry and plant growing wastes.

The disadvantages of the method include its high energy intensity and, as a consequence, its high cost.

The most widespread methods using microorganisms.

A known method of processing bird droppings (RF patent No. 2055823, IPC (6) C05F 11/08, C12P 39/00), comprising introducing into the bird droppings with humidity 80-90% of a consortium of bacteria Streptococcus thermophilus, Streptococcus bovis, Lactobacillus salivarius var salicinicus, Lactobacus salivarius var. salivarius, Lactobacillus acidophilus, deposited in VKPM under N B-5972, in an amount of 0.01-4.0%. The mixture is fermented under natural conditions, then a moisture-absorbing material is introduced into the fermented mixture, which can be used peat or solid-phase litter. Then the mixture is fermented at 60-80 ° C, with aeration and stirring in the presence of larvae of synanthropic flies until the temperature drops to 25-30 ° C, then the above consortium is added in an amount of 0.01-8.0% and fermented again at a temperature the environment. The result is a product that can be used both as a fertilizer and as a feed additive.

A known method of biological processing of bird droppings, involving the mixing of bird droppings with moisture-absorbing material, followed by aerobic fermentation of the mixture in the presence of microorganisms with stirring until the temperature of the fermentation mixture naturally drops to 25-30 ° C. Moreover, as microorganisms, a consortium of strains of Bacillus subtilis B-168, Bacillus mycoides B-691, Bacillus mycoides B-46, Streptococcus thermophilus B-907, Candida tropicalis Y-1520, Candida utilis Y-2441 (RF patent No. 232322727, IPC ( 2006.01) C05F11 / 08, (2006.01) C12N1 / 20). An advantageous implementation of the method of biological processing of bird droppings when a consortium of strains of Bacillus subtilis B-168, Bacillus mycoides B-691, Bacillus mycoides B-46, Streptococcus thermophilus B-907, Candida tropicalis Y-1520, Candida utilis Y- is used as a consortium of microorganisms 2441 in equal proportions and in the amount of 1 · 10 ⁸ -1 · 10 ⁹ cells in 1 ml per 1 ton of bird droppings.

Thus, the claimed method allows to obtain a cheaper high-performance fertilizer by reducing the cost of its processing by simplifying the process and reducing the consumption of a consortium of strains.

The vital products of effective microorganisms (EM) were used to create various biological products aimed at the fast, efficient processing of organic waste into high-quality organic fertilizer; for washing away sewage, spraying equipment and stall premises for farm animals and poultry; treatment of sewage collection tanks for the purpose of disinfection by colonizing putrefactive, pyogenic and pathogenic microflora with microbial antagonists; to reduce the concentration of ammonia and other harmful unpleasant fumes in the air, for example, a biological product from effective microorganisms for the degradation of organic waste (RF patent No. 2347808, IPC C12N 1/20 (2006.01), A01N 63/00 (2006.01). The line of biological products of the EM series - it is a living community of 86 carefully selected beneficial soil microorganisms known worldwide as “effective microorganisms” (http://www.agrotehcom.ru/retailment/renaissance.html).

The disadvantages of all of the above methods for the biological processing of bird droppings, as well as the applied biological products of the EM series, are their multicomponent nature, and as a result, their claimed versatility. The use of these drugs is based on the principle of creating an artificial biocenosis, where there are both bacillary, bacterial and yeast microorganisms, as well as microalgae and protozoa.

The closest analogue to the claimed technical solution is a method of processing poultry manure using the biological product "Tamir" containing active saprophytic microorganisms (Zvezdin V.V. et al. Accelerated utilization of chicken manure and obtaining high-quality fertilizers based on it by biological treatment. Achievements EM- technology in Russia, Issues of the practical application of microbiological preparations Baikal EM 1, Tamir and EM-Kurunga. Collection of works. - M .: 2004). At the exit from the poultry farm, the Tamir biological product was added to the litter, which is taken into the pits, and mixed with the preparation 2 times during the day. The pit is filled for 3 days, and natural mixing occurs. The cost of introducing and mixing the EM product in this case is less than when making it into the field using a tractor and a barrel. When filling trolleys with manure using an excavator, additional mixing takes place. Mixing also continues during transport in carts. Mixed with Tamir biological product, chicken droppings were taken out to the arable field and spread across the field with a thin layer. A week later, this arable land was cultivated, and another two weeks later sowed grain. It has been proven that such technology is applicable year-round. Concentrations and quantities required for the application of the drug "Tamir" are shown in table No. 1 (http://www.em-technology.com.ua/ecology.html)

Table number 1 The scheme of the integrated use of EM to solve environmental problems Processing object A drug Concentration Working solution consumption 1. Wastewater and sewage treatment Tamir, Baikal-EM-1 1:10 10 l / m ³ 2. Organic waste processing, 30-60 days Tamir, Baikal-EM-1 1:10 10 l / t 3. Elimination of bad smell Tamir, Baikal-EM-1 1: 250 1 l / m ² 4. Reducing the content of hydrogen sulfide and nitrogen dioxins in the air Tamir, Baikal-EM-1 1: 250 1 l / m ² 5. Urban soil rehabilitation Tamir, Baikal-EM-1 1: 100 200 l / ha 6. Disinfection of water in reservoirs of recreational and decorative value Tamir, Baikal-EM-1 1: 1000 0.01% of the reservoir volume 7. Sod cultivation Tamir, Baikal-EM-1 1: 1000 200 l / ha 8. Protection of green spaces along highways from the negative impact of the environment Tamir, Baikal-EM-1 1: 1000 200 l / ha 9. Reclamation of city landfills Tamir, Baikal-EM-1 1: 100 10 l / t

The disadvantages of the closest analogue include its duration, as well as all the same disadvantages that are inherent in analogues of microbiological processing of bird droppings, namely multicomponent, versatility, as a result of which the natural biocenosis of bird droppings, which contains all groups of microorganisms introduced with a biological product, is not taken into account "Tamir", which is impractical and unjustified.

The technical task of the proposed method of microbiological processing of bird droppings is to accelerate the bioconversion process with a simultaneous increase in the biological activity of the processed product and environmental safety.

To solve the technical problem, a method for microbiological processing of bird droppings is proposed, which consists in introducing the microbial culture of Pseudomonas sp.114, deposited in VKPM under No. B-5060, into the bird droppings, followed by stirring, and then after 5 days the microbial culture of Azotobacter chroococcum B 35 is introduced deposited in VKPM under No. B - 6010, and again mixed. The titer of the introduced microbial cultures was for Pseudomonas sp.114 - 10 ⁸ cells / ml and for Azotobacter chroococcum B 35 - 10 ⁸ cells / ml. The volumetric ratio of the introduced crops is 2: 1, respectively, at the rate of 45 ml per 1 kg of bird droppings with unprotected bird maintenance. With the litter content of poultry Pseudomonas sp.114 and Azotobacter chroococcum B 35 taken in a ratio of 2: 1, add 15 ml per 1 kg of litter. Before introducing microbial cultures, each of them is diluted with water in a ratio of 1: 2, respectively. After 15 days from the start of processing of bird droppings, an environmentally safe product is obtained with reduced pathogenic microflora and without helminth infection.

In the course of experimental studies, at the first stage, we studied the effect of the Pseudomonas sp.114 culture on the biodegradation of litter of non-litter chickens taken from the Krasnodar poultry farm in an amount of 3 kg.

An active microbial culture of Pseudomonas sp.114 was obtained by growing in a fermenter on glucose-peptone medium (composition in g / l: 3.2 g Na ₂ HPO ₄ × 12H ₂ O, 0.3 g KH ₂ PO ₄ , 0.5 g NaCl, 0.5 g MgSO ₄ , 2 g peptone, 20 g glucose) to a titer of 10 ⁸ cells / ml.

100 ml of the microbial culture of Pseudomonas sp.114, previously diluted in water in a ratio of 1: 2, respectively, was added, mixed and kept for 30 days. Every 5 days, samples were taken and analyzed for the total microbial number (seeding for meat and peptone agar) and for the content of ammonium nitrogen (Petersburg A.V. Workshop on agronomic chemistry. M: Publishing House of Agricultural Literature, - 1963. - 592 p.) The research results are presented in table No. 2.

Table number 2 The dependence of the bioconversion of chicken litter on the processing time of the culture Pseudomonas sp.114 Time from the beginning of sowing, day INDICATORS The total microbial number in chicken manure, cells / ml Ammonium nitrogen, mg / l 0 10 ⁶ 250 5 10 ^{7 g} 195 10 10 ⁹ 165 fifteen 10 ¹⁰ 110 twenty 10 ¹⁰ 115 thirty 10 ¹⁰ 122

According to the test results, it was noted that the greatest number of microbial cells of bird droppings was reached by 15 days after the introduction of the microbial culture of Pseudomonas sp.114.

By 20 and 30 days, the amount of microbial mass did not increase, due to the cessation of the enzyme complex of the culture Pseudomonas sp.114.

To reduce the ammonium nitrogen content, a decrease in this indicator by 15-20 days from the start of treatment was noted, which is consistent with the dynamics of an increase in the total microbial number.

Based on the results obtained, 15 days were selected as the optimal time for keeping bird droppings after the action of the proteolytic culture of Pseudomonas sp.114.

It is known to use microorganisms of the genus Azotobacter to increase soil fertility, biological remediation of soils and enrich it with nitrogen compounds, however, in the presence of ammonia, the nitrogen fixation process stops and the microbial culture of the genus Azotobacter begins to utilize free ammonia, which is rich in bird droppings, which gives a high deodorizing effect (G.N. .Zaitseva. Biochemistry of Azotobacter. M.: Publishing House "Science", 1965, 304 S.).

At the next stage of the experimental studies, we searched for the optimal ratio of microbial cultures of Pseudomonas sp.114 and Azotobacter chroococcum B 35 when processing litter of non-litter chickens taken from the Krasnodar poultry farm in the amount of 3 kg for each of the selected ratios.

Used microbial cultures:

- Pseudomonas sp. 114, grown as described above;

- Azotobacter chroococcum B 35 grown on Ashby's medium (G.N. Zaitseva. Azotobacter biochemistry. M.: Nauka Publishing House, 1965, 304 s).

The microbial culture titer for Pseudomonas sp. 114 was 10 ⁸ cells / ml and

for Azotobacter chroococcum B 35-10 ⁸ cells / ml.

The treatment of chicken litter was carried out initially by the microbial culture of Pseudomonas sp.114, and after 5 days, the microbial culture of Azotobacter chroococcum B 35 was taken, taken in different volume ratios with their total amount equal to 135 ml, previously diluted in water in a ratio of 1: 2, respectively (see Table No. 2-4). After each application of microbial cultures, mixing was carried out. Fifteen days after the start of treatment, analytical and microbiological monitoring of bird droppings was carried out on the dynamics of ammonium nitrogen (Petersburg A.V. Workshop on agronomic chemistry. M: Publishing House of Agricultural Literature, 1963. - 592 pp.) And in terms of the total microbial number (Ashmarin I.I. Practical medical microbiology. M .: Medicine, 1966, - 329 p.).

Table number 3 The effect of the ratio of microbial cultures of Pseudomonas sp.114 and Azotobacter chroococcum B 35 on the effectiveness of bird droppings bioconversion after 15 days for the utilization of ammonium nitrogen and the growth of the microbial number The ratio of cultures of Pseudomonas sp. 114: Azotobacter chroococcum B 35 INDICATORS Ammonium nitrogen, mg / l Total microbial number, cells / ml Before processing After 15 days Before processing After 15 days 1: 1 250 110 10 ⁶ 10 ⁹ 1: 2 250 120 10 ⁶ 10 ¹¹ 1: 3 250 130 10 ⁶ 10 ¹¹ 2: 1 250 90 10 ⁶ 10 ¹⁰ 3: 1 250 140 10 ⁶ 10 ¹⁰

In addition, we determined the number of viable larvae of flies according to MU No. 3.5.2.1759-03 dated September 28, 2003 "Methods for determining the effectiveness of insecticides and acaricides used in medical pest control" and helminthological insemination of bird droppings according to MUK 4.2.796-99 dated 03.22.2002 "Methods sanitary - parasitological studies. " The research results are shown in tables No. 4, 5.

Table number 4 The effect of the ratio of microbial cultures of Pseudomonas sp.114 and Azotobacter chroococcum B 35 on the effectiveness of bird droppings bioconversion after 15 days by changes in the number of coliform bacteria and parasatological contamination The ratio of cultures of Pseudomonas sp. 114: Azotobacter chroococcum B 35 INDICATORS Coliform bacteria Parasitology Before processing After 15 days Before treatment, the number of cysts After 15 days, the number of cysts 1: 1 10 ³ 10 5 2 1: 2 10 ³ 10 ² 5 3 1: 3 10 ³ 10 ² 5 Not found 2: 1 10 ³ 10 ² 5 Not found 3: 1 10 ³ 10 ³ 5 Not found

Table number 5 The effect of the ratio of microbial cultures of Pseudomonas sp.114 and Azotobacter chroococcum B 35 on the effectiveness of bird droppings bioconversion after 15 days on the change in the number of pathogenic microflora and the number of fly larvae The ratio of cultures of Pseudomonas sp. 114: Azotobacter chroococcum B 35 INDICATORS Pathogenic microflora (Salmonella) Larvae of flies Before processing After 15 days Before treatment, in 100 ml After 15 days, in 100 ml 1: 1 10 ² Not found 66 35 1: 2 10 ² Not found 66 28 1: 3 10 ² Not found 66 32 2: 1 10 ² Not found 66 eighteen 3: 1 10 ² Not found 66 36

As follows from the table. No. 3-5 is the optimal ratio of microbial cultures of Pseudomonas sp.114 and Azotobacter chroococcum B 35 taken in a ratio of 2: 1, respectively.

Example 1

In the period from December 2 to December 17, 2009 Microbiotech LLC on the basis of the Krasnodar poultry farm (Loris village) organized and carried out a pilot processing of 25 tons of uncontaminated poultry manure at the poultry farm litter.

The experimental collar of the litter storage was treated with the microbial culture Pseudomonas sp.114, grown on glucose-peptone medium with a titer of 10 ⁸ cells / ml and diluted with water in a ratio of 1: 2, in an amount of 800 l. The existing equipment of the Krasnodar poultry farm was used. For the introduction of the biological product, a spraying plant of the poultry factory design (modified sprayer for gardening) was used, a bucket loader was used for mixing. After 5 days using the same equipment, the experimental collar was treated with the microbial culture Azotobacter chroococcum B 35 grown on molasses medium (composition per 1 l: 45 g beet molasses, 2 g KH ₂ PO ₄ , 0.02 g yeast extract) with a titer 10 ⁸ cells / ml and diluted with water in a ratio of 1: 2, in an amount of 400 l, December 7, 2009

During the tests, the temperature and humidity of the shoulders, the biological component were controlled, the physicochemical analysis of the initial litter was carried out, and then the physicochemical analysis of the final product. The control of the biological component was carried out once a week. They noted what happens with the litter, how the pathogenic microflora decreases and the beneficial microflora increases. Visual monitoring of the appearance of bird droppings was also conducted. During the tests, a gradual change in the color and state of aggregation of the contents of the shoulders and a decrease in ammonia odor were observed.

Samples were taken on the first day of application, after 6 days and 15 days from the start of treatment and analytical, microbiological and parasitological control was carried out in accordance with the methods described above. As a result of the application of the proposed method, the following results are obtained, which are reflected in tables No. 6, 7.

Table number 6 Results on the change in the total microbial number, ammonium nitrogen, coliform bacteria and pathogenic microflora The time from the start of processing day Indicators Total microbial number, cells / ml Ammonium nitrogen, mg / l Coliform microorganisms CFU, cells / ml Pathogenic microflora (Salmonella), cells / ml To start 10 ⁶ 330 10 ³ 300 6 days 10 ⁸ 250 10 ² 24 15 days 10 ¹¹ 120 10 ² Not found

Table number 7 The results of changes in the number of larvae of flies and parasitological infection The time from the start of processing, day Indicators Parasitology number, cysts in 1 l Larvae of flies, In 100 ml To start 12 45 6 days 6 21 15 days 0 fourteen

In addition to the results shown in tables No. 6.7, it should be noted that there was a decrease in the content of harmful gases in the air: hydrogen sulfide, ammonia, methane, and laboratory tests and bioassays established a hazard class of 5 (4) processed bird droppings.

Bird droppings, not processed in accordance with the claimed method and located next to the litter storage, did not undergo any changes, remained a sticky mass of brown-green color with an unpleasant odor.

Example 2

In the period from October 17, 2009 to November 1, 2009, 40 kg of litter of hens from the litter of the Adler poultry farm was organized and processed. On October 17, 2009, the litter of litter chickens was treated with the microbial culture Pseudomonas sp.114, grown on glucose peptone medium with a titer of 10 ⁸ cells / ml and diluted with water in a ratio of 1: 2, followed by stirring. After 5 days, the litter was treated with the microbial culture Azotobacter chroococcum B 35 grown on molasses medium (composition per 1 l: 45 g beet molasses, 2 g KH ₂ PO ₄ , 0.02 g yeast extract) with a titer of 10 ⁸ cells / ml and diluted with water in a ratio of 1: 2, followed by stirring. In the course of the experiment, the quantities of microbial cultures, both previously selected and smaller, were used, due to the composition of the litter, which is characterized by a high content of cellulose-containing components (straw, sunflower husk). Under the influence of the culture of Pseudomonas sp.114, the development of cellulose-destroying microorganisms (Cellulumonas, Trichoderma) is stimulated, and therefore, a sufficient amount of sugar hydrolyzed from cellulose is formed, which is an additional stimulating effect for the development of native bird droppings. After 15 days, i.e. On November 1, 2009, in accordance with the above methods, analytical, microbiological and parasitological control was carried out.

Table number 8 The effect of the number of introduced microbial cultures of Pseudomonas sp.114 and Azotobacter chroococcum B 35 on the efficiency of bioconversion of bird droppings in the litter of chickens after 15 days for the utilization of ammonia nitrogen and the growth of the microbial number The number of introduced cultures of Pseudomonas sp.114 and Azotobacter chroococcum in 35 ml / kg Indicators Total microbial number, cells / ml Ammonium nitrogen, mg / l Before processing After 15 days Before processing After 15 days 30 + 15 ml / kg 10 ⁶ 10 ¹¹ 350 55 20 + 10 ml / kg 10 ⁶ 10 ¹¹ 350 60 10 + 5 ml / kg 10 ⁶ 10 ¹¹ 350 60 5 + 2.5 ml / kg 10 ⁶ 10 ⁸ 350 95

Table number 9 The effect of the number of introduced microbial cultures of Pseudomonas sp.114 and Azotobacter chroococcum B 35 on the efficiency of bioconversion of bird droppings in the litter content of chickens after 15 days in terms of the decrease in pathogenic microflora and the content in the litter of fly larvae The number of introduced cultures of Pseudomonas sp.114 and Azotobacter chroococcum B 35, ml / kg INDICATORS Pathogenic microflora (Salmonella) Larvae of flies Before processing After 15 days Before treatment, in 100 ml After 15 days, in 100 ml 30 + 15 ml / kg Not found Not found 42 16 20 + 10 ml / kg Not found Not found 42 fourteen 10 + 5 ml / kg Not found Not found 42 eleven 5 + 2.5 ml / kg Not found Not found 42 12

Table number 10 The effect of the number of introduced microbial cultures of Pseudomonas sp.114 and Azotobacter chroococcum B 35 on the efficiency of bioconversion of bird droppings in the litter of chickens after 15 days in terms of coliform bacteria and parasitological infection The number of introduced cultures of Pseudomonas sp.114 and Azotobacter chroococcum B 35, ml / kg INDICATORS Coliform bacteria Parasitology Before processing After 15 days Before treatment, 1 liter cyst After 15 days, cyst in 1 l 30 + 15 ml / kg 10 ³ 10 ² Not found Not found 20 + 10 ml / kg 10 ³ 10 ² Not found Not found 10 + 5 ml / kg 10 ³ 10 ² Not found Not found 5 + 2.5 ml / kg 10 ³ 10 ² Not found Not found

As the analysis of the data obtained in tables No. 8-10 shows, despite the reduced number of introduced crops (in the case of litter of litter), it was revealed that the stimulating effect for native microflora persists even with such a quantity of two microbial cultures.

Comparing the results obtained with the processing of chicken droppings of various contents by microbial cultures, it should be noted that when processing bird droppings, the quantitative ratios of the microbial cultures of Pseudomonas sp.114 and Azotobacter chroococcum B 35 in all cases should be 2: 1, and their total number should be in three times less when processing bird droppings of litter of hens (see table. 11).

Table number 11 Comparative characteristics of the bioconversion of chicken litter with litter and bedding kept by microbial cultures of Pseudomonas sp.114 and Azotobacter chroococcum B 35 15 days after treatment The number of introduced cultures of Pseudomonas sp.114 and Azotobacter chroococcum B 35, ml / kg Indicators The increase in the total microbial number, cells / ml Ammonium nitrogen reduction, mg / l Coliform bacteria reduction Reduced flies Litterless litter, 30 + 15 ml / kg Up to 10 ¹¹ Up to 90 Up to 10 ² 3 times Litter, 10 + 5 ml / kg Up to 10 ¹¹ Up to 60 Up to 10 ² 4 times

Patent information studies have shown that the proposed combination of essential features has novelty, inventive step and is industrially applicable. The technical task is solved. The proposed method of microbiological processing of bird droppings can be recognized as an invention.

Claims (4)

1. The method of microbiological processing of bird droppings using microbial cultures diluted in water in certain proportions and introduced into the bird droppings, followed by mixing, for a certain period, characterized in that Pseudomonas sp. 114, deposited in VKPM under No. B-5060, and Azotobacter chroococcum B 35, deposited in VKPM under No. B-6010, taken in a 2: 1 ratio with each titer of 10 ⁸ cells / ml, each of which was previously diluted in water in a ratio of 1: 2, respectively, moreover, microbial cultures are introduced into bird droppings sequentially, starting with the introduction of Pseudomonas sp. 114 followed by stirring, after 5 days - Azotobacter chroococcum B 35 and mix. 2. The method according to claim 1, characterized in that the microbial culture of Pseudomonas sp. 114 and Azotobacter chroococcum B 35 are added in an amount of 45 ml per 1 kg of droppings with non-laying poultry. 3. The method according to claim 1, characterized in that the microbial culture of Pseudomonas sp. 114 and Azotobacter chroococcum B 35 are added in an amount of 15 ml per 1 kg of litter with litter content of the bird. 4. The method according to any one of claims 1 to 3, characterized in that the microbiological processing of bird droppings is carried out for 15 days.