CN105176866A - Composite microbial flora and enzyme product thereof, and method of applying composite microbial flora and enzyme product thereof in soil restoration - Google Patents
Composite microbial flora and enzyme product thereof, and method of applying composite microbial flora and enzyme product thereof in soil restoration Download PDFInfo
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Abstract
The invention provides a composite microbial flora and an enzyme product thereof, and a method of applying the composite microbial flora and the enzyme product thereof in soil restoration. The composite microbial flora is composed of a microbial flora and a bioactive enzyme, wherein the microbial flora is composed of bacillus subtilis, bacillus mucilaginosus, bacillus amyloliquefaciens, pediococcus pentosaceus, pichia farinose and pediococcus acidilactici, and the bioactive enzyme is composed of phytase, protein hydrolase, cellulose hydrolase and urease. Compared with the prior art, an effective microbial community and a microecological environment are constructed to realize biological catalysis and improve the content of organic matters and beneficial microbial flora in soil, and thus, the fertility of the soil is improved; biochemical degradation rate of soil pollutant can be substantially accelerated, digestion and biodegradation of the pollutant can be accelerated, and final inertial products like carbon dioxide, water and nitrogen are obtained; and toxic intermediate produced in the process of biochemical degradation can be rapidly removed, odor is eliminated, and emission of organic volatile matters (VOC) is reduced.
Description
Technical field
The present invention relates to microbial soil conditioner technical field, particularly relate to complex microbial community and enzyme product thereof and the application method in rehabilitating soil.
Background technology
Modern agriculture production requires the Sustainable development of agricultural.Content with the soil organism while agriculture Sustainable development requires soil fertility to improve constantly increases, and the sustainability of agricultural is alternatively the sustainability of soil.The sustainability index of soil comprises soil nutrient elements, diversity of soil microorganism, biomass, biological fitness, biologically stable, enzyme multiplicity etc.
Soil is made up of mineral substance, organic matter and microorganism three parts, is the basis of crop growthing development.Beneficial microorganism in soil participates in the conversion of matter and energy in soil directly, a series of soil fertility such as formation Sum decomposition, the release of nutrient, the fixing of nitrogen of soil ulmin is formed and growth course.Adopt artificial mode to increase quantity of useful microbe in soil, just can strengthen the activity of Soil Microorganism, thus improve the fertility of soil.Bio-bacterial manure is now emerging a kind of goods based on living microorganisms, due to the characteristic of its green, safety, environmental protection, is conducive to the Sustainable development of modern agriculture, in modern agricultural production, more and more receives publicity.Bio-bacterial manure contains a large amount of microorganisms, by the vital movement of microorganism in soil, improves the nutritional condition of crop.Particularly the microorganism active of farm crop rhizosphere soil micro-ecosystem system is then even more important to plant root nutrition, therefore, use microbial fertilizer at plant rhizosphere, just can increase quantity and the activity of the probiotics in rhizosphere soil, cause the enhancing of soil fertility, improve crop growth environment.
Conventional agriculture has caused very havoc to soil.One, the pest management of nonstandardized technique, make a part of peasant when crop pest, treat the head when the head aches, pin pain cure pin, allow a large amount of agricultural chemicals is unconfined enters soil, the eubiosis (as profitable strain, beneficial organism) of soil is destroyed, for even more serious disease hides some dangers for while allowing crop pest obtain temporarily control; Its two, nonstandard fertilizer application, makes a lot of agriculture practitioner in soil, drop into the macroelement such as the directly related nitrogen of a large amount of volume increase, phosphorus, potassium, make fertilizer efficiency from every kilogram volume increase 15-25Kg taper to 5-8Kg, soil fertility degradation.A large amount of uses of these traditional chemical agricultural chemicals and chemical fertilizer, serious destruction is caused to Agricultural Soil Environment for a long time, make the quality of agricultural land soil occur sharply declining, the problem such as the soil salinization, pesticide residue, heavy metal contamination and then highlight, widespread pollution from the overuse of fertilizers and pesticides in rural area problem is day by day serious.
The agrochemicals applied in modern agriculture, be all generally " specially preventing specially controlling ", this just makes agricultural apply a large amount of, various chemical at each growth phase of crop, guarantees that crop obtains comprehensively protect with this.But, as this method will make a large amount of chemical enter into agricultural land soil, serious destruction is caused to soil, is unfavorable for the Sustainable development of agricultural, but also can cause that pesticide residue exceed standard, a series of problem of degradation under agricultural product quality.Thus, the exploitation of multipotency composite farm product, also becomes the active demand of modern agriculture.
Summary of the invention
For above-mentioned defect and the problem of soil quality decline and serious environmental pollution in prior art, the object of the embodiment of the present invention is to provide a kind of complex microbial community of better reliability and enzyme product thereof and the application method in rehabilitating soil, repair contaminated soil, improve soil, increase soil fertility and further coordinate plant growth, raising plant biomass, control plant pest, raising disease resistance of plant.
In order to achieve the above object, the embodiment of the present invention provides following technical scheme:
A kind of complex microbial community and enzyme product thereof, is characterized in that, be made up of microorganism species and bioactive enzyme:
Microorganism species is made up of subtilis, bacillusmusilaginosiengineering, bacillus amyloliquefaciens, Pediococcus pentosaceus, pichia farinose and pediococcus acidilactici; Bioactive enzyme is made up of phytase, proteolytic enzyme, cellulolytic enzyme and urase.
Preferred as technique scheme, in described microorganism species, with total viable count for benchmark, wherein, the 10%-35% that the viable count of subtilis is the 10%-35% of total viable count, the viable count of bacillusmusilaginosiengineering is the 10%-35% of total viable count, the viable count of pichia farinose is the 10%-35% of total viable count, the viable count of bacillus amyloliquefaciens is the 10%-35% of total viable count, the viable count of Pediococcus pentosaceus is the 10%-35% of total viable count, the viable count of pediococcus acidilactici is total viable count.
Preferred as technique scheme, in described microorganism species, with total viable count for benchmark, wherein, the viable count of subtilis is 35% of total viable count, the viable count of bacillusmusilaginosiengineering is 15% of total viable count, the viable count of pichia farinose is 10% of total viable count, the viable count of bacillus amyloliquefaciens is 10% of total viable count, the viable count of Pediococcus pentosaceus is 15% of total viable count, the viable count of pediococcus acidilactici is 15% of total viable count.
Preferred as technique scheme, by weight percentage, its ratio value is the ratio of the phytase of described bioactive enzyme, proteolytic enzyme, cellulolytic enzyme and urase: phytase: proteolytic enzyme: cellulolytic enzyme: urase is 2-5:1-3:2-4:1.5-3.5; Be preferably 4:1:3:2.
Preferred as technique scheme, described microorganism species by subtilis, bacillusmusilaginosiengineering, bacillus amyloliquefaciens, Pediococcus pentosaceus, pichia farinose and pediococcus acidilactici after fermentation culture, or directly to use or the concentrated viable bacteria goods using or make through carrier adsorption.
Preferred as technique scheme, the content range of the activeconstituents of each microbial bacteria of described microorganism species is 1 × 10
7cfu/g-1 × 10
8cfu/g, optimal way is, bacillus subtilis bacterial content is 6 × 10
7the content of cfu/g, bacillusmusilaginosiengineering is 2 × 10
7the content of cfu/g, pichia farinose is 5 × 10
7the content of cfu/g, bacillus amyloliquefaciens is 6 × 10
7the content of cfu/g, Pediococcus pentosaceus is 4 × 10
7the content of cfu/g, pediococcus acidilactici is 8 × 10
7cfu/g.
Preferred as technique scheme, described microorganism species exists with the form of the fermented liquid of viable cell, viable cell by cultivating, the filtrate of cell culture or the mixture of cell and filtrate.
Preferred as technique scheme, described complex microbial community and enzyme product thereof also comprise carrier, carrier be solid carrier and or liquid vehicle: described solid carrier can be mineral material, biomaterial and or macromolecular compound; Described mineral material is at least one in clay, talcum, kaolin, montmorillonite, white carbon, zeolite, silica, turfy soil and diatomite; Clay take attapulgite as the clay mineral of main component, and biomaterial is at least one in the ight soil of the stalk of all kinds of crop, loose shell, straw, Pericarppium arachidis hypogaeae, Semen Maydis powder, bean powder, starch, the peat composed of rotten mosses and animal; Macromolecular compound is polyvinyl alcohol and/or polyglycol; Described liquid vehicle is organic solvent, vegetables oil, mineral oil, clay or water; Described organic solvent is decane and or dodecane.
Preferred as technique scheme, described complex microbial community and enzyme product thereof also comprise tensio-active agent, tackiness agent, stablizer and antioxidant, pH adjusting agent; Tensio-active agent is polysorbas20 or tween 80.
Preferred as technique scheme, described complex microbial community and enzyme product thereof are soil-repairing agent or microbial fertilizer, are applied to preparation and repair polluted soil and or improvement soil and or increasing soil fertility and or Promoting plant growth.
Meanwhile, the invention provides the preparation method of the pulvis of complex microbial community and enzyme product thereof, it is characterized in that, step is as follows:
Q1: the activation of bacterium and enlarged culturing:
Activation: be seeded in respectively on substratum by each microbial bacteria of microorganism species, be placed in 28 DEG C of incubators and cultivate 5 days, obtains the microorganism species activated;
Enlarged culturing: the fermentor tank microorganism species of activation being accessed respectively substratum, all carries out static enlarged culturing step by step at 28 DEG C, and in enlarged culturing process, inoculum size is volume percent content 5%-10%; Obtain the fermented liquid 1 × 108cfu/g of microorganism species respectively;
Q2: the preparation of pulvis: by the fermented liquid of the microorganism species that Q1 step obtains, carries out being mixed to get fermentation mixed solution in colony forming units cfu number ratio; In fermentation mixed solution, the viable count of subtilis is 35% of total viable count, the viable count of bacillusmusilaginosiengineering is 15% of total viable count, the viable count of pichia farinose is 10% of total viable count, the viable count of bacillus amyloliquefaciens is 10% of total viable count, the viable count of Pediococcus pentosaceus is 15% of total viable count, the viable count of pediococcus acidilactici is 15% of total viable count;
Mix according to weight ratio 2:3:2:2:1 with turfy soil, diatomite, Semen Maydis powder, starch, attapulgite clay, obtained microbial bacteria agent carrier;
Q3: with phytase: proteolytic enzyme: cellulolytic enzyme: urase mixes according to weight ratio 4:1:3:2, add Q2 step the microbial bacteria agent carrier obtained and the mixed solution that ferments, keep total water content to be 70%, 30 DEG C of quiescent culture 3 days during mixing, 35 DEG C of naturally ventilated dryings obtain composite microbial bacteria enzyme system pulvis.
Preferred as technique scheme, the application method of pulvis in rehabilitating soil of complex microbial community and enzyme product thereof, it is characterized in that, method steps is as follows:
Q1: be blended into by composite microbial bacteria enzyme system pulvis in warm water and leave standstill to activate medicament, the time is 12-24 hour;
Q2: the pulvis aqueous solution after activating is divided into 3 parts, a part evenly spray arrives soil table; Wholely, fertilising soil layer is ploughed under below earth's surface, to protect microbial inoculum not by excessive illumination effect;
Q3: on field planting same day, the pulvis aqueous solution after the activation of part 2 is evenly sprayed soil table; Wholely, fertilising soil layer is ploughed under below earth's surface, to protect microbial inoculum not by excessive illumination effect;
Q4: after field planting the 4th day, the 27th day, use the aqueous solution of the composite microbial bacteria enzyme system pulvis of dilution 200 times;
Q4: after field planting 29 days, the pulvis aqueous solution after the activation of the 3rd part is applied by plants stems stalk lower end mulch film opening part.
The complex microbial community that the embodiment of the present invention provides and enzyme product thereof and the application method in rehabilitating soil, compared with prior art, can by set up profitable strain, build micro-ecological environment realize biocatalysis, improve soil organic matter content and beneficial microbe colony, and then increase soil fertility.Significantly can accelerate the biochemical degradation speed of soil pollutant, clearing up and biological degradation of pollutent can be accelerated, to final inert products, as carbonic acid gas, water, nitrogen etc.; And the toxic intermediate produced in biochemical degradation process can be removed fast, the release of deodorizing and minimizing organic volatile (VOC).
Beneficial effect of the present invention:
(1) exploitation that the complex microbial community developed of the present invention and enzyme product thereof are multiple-microorganism bacterial manure provides basis, produces provide possibility for diversified bacterial manure.
(2) complex microbial community developed of the present invention and enzyme product thereof have multiple effect, can be crop and provide multiple-protection, can reduce the amount of application of agrochemicals.
(3) complex microbial community developed of the present invention and enzyme product thereof are 100% pure natural organic preparation, harmless, free from environmental pollution, can solve the environmental problem that farm artificial Pollution by Chemicals brings.
Can field planting survive after complex microbial community of the present invention and enzyme product thereof are manured into soil, rehabilitating soil environment, has positive effect to promotion plant growth.
Accompanying drawing explanation
In order to be illustrated more clearly in the embodiment of the present invention or technical scheme of the prior art, be briefly described to the accompanying drawing used required in embodiment or description of the prior art below, apparently, accompanying drawing in the following describes is only some embodiments of the present invention, for those of ordinary skill in the art, under the prerequisite not paying creative work, other accompanying drawing can also be obtained according to these accompanying drawings.
Fig. 1 is that the complex microbial community of the complex microbial community of the embodiment of the present invention and the different concns of enzyme product and the application in rehabilitating soil thereof and enzyme product thereof are to the degradation effect schematic diagram of agricultural chemicals.
Fig. 2 is the degradation effect figure that the complex microbial community of the embodiment of the present invention 2 and enzyme product thereof make to 2,4-D in rehabilitating soil after bacterial manure.
Embodiment
Be clearly and completely described technical scheme of the present invention below, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
In one embodiment of the invention, described subtilis, described bacillusmusilaginosiengineering, described pichia farinose, described solution starch gemma bar, described Pediococcus pentosaceus and described pediococcus acidilactici can exist with the form of the fermented liquid of viable cell, viable cell by cultivating, the filtrate of cell culture or the mixture of cell and filtrate.
Subtilis specifically can choose common bacterial classification, also can choose the preservation of bacteria strain etc. of China General Microbiological preservation administrative center, such as, be numbered the subtilis (Bacillussubtilis) of CGMCCNo.1.1630; Bacillusmusilaginosiengineering specifically can be the bacillusmusilaginosiengineering (Bacillusmucilaginosus) being numbered CGMCCNo.1.232; Bacillus amyloliquefaciens specifically can be the bacillus amyloliquefaciens (Bacillusamyloliquefacicns) being numbered CGMCCNo.8341; Pediococcus pentosaceus specifically can be the Pediococcus pentosaceus (Pediococcuspentosaceus) being numbered CGMCCNo.7049; Pediococcus acidilactici specifically can be the pediococcus acidilactici (Pediococcusacidilactici) being numbered CGMCCNo.9441; Pichia farinose specifically can be the pichia farinose (Pichiafarinosa) being numbered CGMCCNo.4008.
Cfu is colony-forming unit.
The preparation of the pulvis of embodiment 1 complex microbial community and enzyme product thereof
1, the activation of bacterium and enlarged culturing
Activation: be seeded in by subtilis on No. 1 substratum, be placed in 28 DEG C of incubators and cultivate 5 days, obtains the subtilis activated; No. 1 substratum is corn agar medium; Such as the substratum of China General Microbiological culture presevation administrative center (being called for short CGMCC) is numbered the corn agar medium of 2006;
Bacillusmusilaginosiengineering is seeded on No. 2 substratum, is placed in 28 DEG C of incubators and cultivates 5 days, obtain the bacillusmusilaginosiengineering activated; No. 2 substratum are A Shi nitrogen-free agar, and such as the substratum of China General Microbiological culture presevation administrative center (being called for short CGMCC) is numbered the A Shi nitrogen-free agar of 0073;
Pichia farinose is seeded on No. 3 substratum, is placed in 28 DEG C of incubators and cultivates 5 days, obtain the pichia farinose activated; No. 3 substratum are LB substratum, and such as the substratum of China General Microbiological culture presevation administrative center (being called for short CGMCC) is numbered the LB substratum of 0033;
Bacillus amyloliquefaciens is seeded on No. 1 substratum, is placed in 28 DEG C of incubators and cultivates 5 days, obtain the bacillus amyloliquefaciens activated; No. 1 substratum is corn agar medium, and such as the substratum of China General Microbiological culture presevation administrative center (being called for short CGMCC) is numbered the corn agar medium of 2006;
Pediococcus pentosaceus is seeded on No. 4 substratum, is placed in 28 DEG C of incubators and cultivates 5 days, obtain the Pediococcus pentosaceus activated; Pediococcus acidilactici is seeded on No. 4 substratum, is placed in 28 DEG C of incubators and cultivates 5 days, obtain the pediococcus acidilactici activated.No. 4 substratum are MRS substratum, and such as the substratum of China General Microbiological culture presevation administrative center (being called for short CGMCC) is numbered the MRS substratum of 0006.
Enlarged culturing: the pediococcus acidilactici of the Pediococcus pentosaceus of the bacillus amyloliquefaciens of the pichia farinose of the bacillusmusilaginosiengineering of the subtilis of above-mentioned activation, activation, activation, activation, activation, activation is accessed the fermentor tank that 6 are equipped with corresponding substratum respectively, all carry out static enlarged culturing step by step at 28 DEG C, in enlarged culturing process, inoculum size is 5%-10% (volume percent content), obtains fermentation of bacillus subtilis liquid (1 × 10 respectively
8cfu/g), the fermented liquid (1 × 10 of bacillusmusilaginosiengineering
8cfu/g), the fermented liquid (1 × 10 of pichia farinose
8cfu/g), the fermented liquid (1 × 10 of bacillus amyloliquefaciens
8cfu/g), the fermented liquid (1 × 10 of Pediococcus pentosaceus
8cfu/g), the fermented liquid (1 × 10 of pediococcus acidilactici
8cfu/g).
2, the preparation of composite microbial bacteria enzyme system pulvis
Colony-forming unit (cfu) the number ratio of the fermented liquid of the fermentation of bacillus subtilis liquid obtained in above-mentioned steps, bacillusmusilaginosiengineering fermented liquid, pichia farinose fermented liquid, bacillus amyloliquefaciens, Pediococcus pentosaceus fermented liquid and pediococcus acidilactici fermented liquid is carried out being mixed to get fermentation mixed solution; In this fermentation mixed solution, bacillus subtilis bacterial content is 6 × 10
7the content of cfu/g, bacillusmusilaginosiengineering is 2 × 10
7the content of cfu/g, pichia farinose is 5 × 10
7the content of cfu/g, bacillus amyloliquefaciens is 6 × 10
7the content of cfu/g, Pediococcus pentosaceus is 4 × 10
7the content of cfu/g, pediococcus acidilactici is 8 × 10
7cfu/g.
By absorption carrier, (this absorption carrier is turfy soil, diatomite, Semen Maydis powder, starch, attapulgite clay mix with weight ratio 2:3:2:2:1, obtained microbial bacteria agent carrier) and above-mentioned fermentation mixed solution and bioactive enzyme (phytase: proteolytic enzyme: cellulolytic enzyme: urase is the mixed solution of the ratio of 4:1:3:2 according to weight ratio) mix, total water content is kept to be 70%, 30 DEG C of quiescent culture 3 days, after fully adsorbing, 35 DEG C of naturally ventilated dryings obtain the pulvis of complex microbial community and enzyme product thereof.
The application of the pulvis of embodiment 2 complex microbial community and enzyme product thereof, to soil remediation effect measuring
(1) reagent agent
The complex microbial community stated obtained according to the embodiment of the present invention 1 and the pulvis of enzyme product thereof, with being dissolved in warm water to activate bacterial classification before using, obtain the bio-bacterial manure medicament that complex microbial community and enzyme product thereof are made.
(2) background is tested
Current soil pollution problem is more and more serious, and current soil remediation scheme or with high costs, or maximum acceptable restriction (50 mgs/kg) is reached by regularly upset in 9 to 12 months within a long period.In the face of current problem, for measuring bio-bacterial manure preparation that complex microbial community of the present invention and enzyme product thereof make to the repairing effect of polluted soil, spy carries out following test.
(3) test design
A) ethoprophos is got, phorate, diazinon, Malathion, methidathion, anilofos, triazophos liquid 50 μ L, pesticide standard liquid concentration is respectively add the bio-bacterial manure medicament that the complex microbial community of 250 μ L6 kind concentration and enzyme product thereof make in 10mg/Kg liquid of the same race (concentration is respectively 0, 25, 50, 100, 200, 400mg/Kg), add 200 μ L water again, after reaction 24h, add 450 μ L acetonitriles and make enzyme deactivation, carry out micro-extraction organism and extract operation, 300 μ L supernatant liquids are extracted out with liquid-transfering gun after stratification, add 300 μ L dilution in acetonitrile one glass again, sample introduction, each sample concentration is analyzed with high performance liquid chromatograph.
B) take the average soil containing 2, the 4-D pollutents of 8000 mgs/kg of 10Kg to do to accelerate to revise test.Polluted soil and water are mixed into 100ppm solution (every cubic metre containing 100g), and add nitrogen, nitrogen nutrient source (increasing fertilizer fast) abundant needed for microorganism by 2000 mgs/kg, ensure that moisture maintains 40%-50% at duration of test.When bio-bacterial manure spray solution is after soil, all material all thoroughly mixes.To guarantee in test that moisture content is greater than 40%.
(4) test-results
After reaction 24h, different concns complex microorganism zymin is different to the degradation effect of each former medicine.Experimental result is shown in Fig. 1 and the following table data of Figure of description:
The cylindricality often organized in Fig. 1 on agricultural chemicals is the degradation effect that enzyme concn is followed successively by 400ppm-0ppm.The ordinate zou of Fig. 1 be to the degraded of each former agricultural chemicals after pesticide concentration value, X-coordinate is each agricultural chemicals.Result obviously shows, and the concentration value being applied with the agricultural chemicals of the embodiment of the present invention obviously reduces.
Note: ppm is mg/Kg
The effect of sampling display removal 2,4-D pollutent is as shown in following table and Fig. 2, and this bacterial fertilizer products can use in position, quick removal pollutent, and application program is simple, use safety, effectively can reduce the use of labour and machine, thus reduce cost, obtain high cost income:
Fig. 2 is the degradation effect figure that the complex microbial community of the embodiment of the present invention 2 and enzyme product thereof make to 2,4-D in rehabilitating soil after bacterial manure; In figure, X-coordinate is the time, and left ordinate zou is 2,4-D concentration in soil, and right ordinate zou is that 2,4-D clears up rate, demonstrates complex microorganism-zymin to 2, the fast degradation effect of 4-D.
| Start | 8000mg/kg |
| 4th week | 350mg/kg |
| 6th week | 33.9mg/kg |
Embodiment 3 complex microbial community and enzyme product thereof, to the manure trial of tomato in plastic greenhouse
(1) reagent agent
The pulvis made according to composite microbial bacteria enzyme system of the present invention and aqua.
(2) test design
In a warmhouse booth, the tomato plant of the quantitative seat number of random selecting is as experimental group and control group, and experimental group is divided into two groups, manages with identical field management measure.Experimental group adopts two kinds of application programs, when application program 1 is for using bacterial manure at every turn, adds 50g nitrogenous fertilizer; Application program 2, for normally to use bacterial manure, applies the water of equivalent at control group.Bacterial manure administration records is as follows:
First 4 days of field planting, experimental group and control group organic fertilizer, and plough;
First 2 days of field planting, experimental group 39#-50# seat:
150g pulvis being shifted to an earlier date (12 hours) is blended in 60L warm water standing to activate medicament; The pulvis aqueous solution after activating evenly is sprayed soil table;
Wholely, fertilising soil layer is ploughed under below earth's surface, to protect microbial inoculum not by excessive illumination effect;
Field planting same day, experimental group 26#-38# seat:
150g pulvis being shifted to an earlier date (12 hours) is blended in 60L warm water standing to activate medicament;
The pulvis aqueous solution after activating evenly is sprayed soil table;
Wholely, fertilising soil layer is ploughed under below earth's surface, to protect microbial inoculum not by excessive illumination effect;
After field planting 4 days, experimental group used 53mL1 aqua (dilute 200 times, use after fully stirring);
After field planting 27 days, experimental group used 53mL aqua (dilute 200 times, use after fully stirring);
After field planting 29 days (bearing fruit), experimental group 26#-50# seat:
100g pulvis being shifted to an earlier date (12 hours) is blended in 60L warm water standing to activate medicament;
The pulvis aqueous solution after activating is applied by plants stems stalk lower end mulch film opening part.
Result viewing
When application program 1 is for using bacterial manure at every turn, add 50g nitrogenous fertilizer; Application program 2, for normally to use bacterial manure, applies the water of equivalent at control group;
The indexs such as, bear fruit number, annesl fruit number thick to tomato stem have carried out observing statistics, and data logging sees the following form:
Following table is the data logging form that the pulvis made of composite microbial bacteria enzyme system and aqua affect Growth of Tomato Seedling:
To be the pulvis made of composite microbial bacteria enzyme system and aqua to bear fruit quantity statistics form to tomato Sansui fruit following table:
Data results shows, within 69 days after field planting, choose plant first fringe fruit and carry out annesl when observing, the overall annesl of experimental group plant first fringe fruit is more than half, and control group first fringe fruit does not also start annesl.Tracking investigation is carried out to annesl situation, finds control group annesl average leg about about 5 days.
Added up by experimental observation, on the plant of the experimental group used at microbial inoculum, compared to control group plant, have following advantage:
Root: experimental group comparatively control group plant root has considerable change, root amount is larger, and side root is flourishing;
Stem: experimental group base of the plant increases thick 18.3%, and significant difference is obvious;
Bear fruit: experimental group Sansui, the 4th fringe fruit amount of bearing fruit improve more than 27.97%;
Annesl: experimental group first fringe fruit plant annesl comparatively control group about 5 days in advance.
In sum, can plant root growth be promoted according to the bio-bacterial manure that composite microbial bacteria enzyme system of the present invention processes, also prove that bio-bacterial manure is to the improved effect of soil, and truly have the effect promoting vine growth and development.
The above; be only the specific embodiment of the present invention, but protection scope of the present invention is not limited thereto, is anyly familiar with those skilled in the art in the technical scope that the present invention discloses; change can be expected easily or replace, all should be encompassed within protection scope of the present invention.Therefore, protection scope of the present invention should described be as the criterion with the protection domain of claim.
Claims (10)
1. complex microbial community and an enzyme product thereof, is characterized in that, is made up of microorganism species and bioactive enzyme:
Microorganism species is made up of subtilis, bacillusmusilaginosiengineering, bacillus amyloliquefaciens, Pediococcus pentosaceus, pichia farinose and pediococcus acidilactici; Bioactive enzyme is made up of phytase, proteolytic enzyme, cellulolytic enzyme and urase.
2. a kind of complex microbial community according to claim 1 and enzyme product thereof, is characterized in that, in described microorganism species, with total viable count for benchmark, wherein, the viable count of subtilis is the 10%-35% of total viable count, the viable count of bacillusmusilaginosiengineering is the 10%-35% of total viable count, the viable count of pichia farinose is the 10%-35% of total viable count, the viable count of bacillus amyloliquefaciens is the 10%-35% of total viable count, the viable count of Pediococcus pentosaceus is the 10%-35% of total viable count, the viable count of pediococcus acidilactici is the 10%-35% optimal way of total viable count is that the viable count of subtilis is 35% of total viable count, the viable count of bacillusmusilaginosiengineering is 15% of total viable count, the viable count of pichia farinose is 10% of total viable count, the viable count of bacillus amyloliquefaciens is 10% of total viable count, the viable count of Pediococcus pentosaceus is 15% of total viable count, the viable count of pediococcus acidilactici is 15% of total viable count.
3. a kind of complex microbial community according to claim 1 and enzyme product thereof, it is characterized in that, by weight percentage, its ratio value is the ratio of the phytase of described bioactive enzyme, proteolytic enzyme, cellulolytic enzyme and urase: phytase: proteolytic enzyme: cellulolytic enzyme: urase is 2-5:1-3:2-4:1.5-3.5; Be preferably 4:1:3:2.
4. preferred as technique scheme, described microorganism species by subtilis, bacillusmusilaginosiengineering, bacillus amyloliquefaciens, Pediococcus pentosaceus, pichia farinose and pediococcus acidilactici after fermentation culture, or directly to use or the concentrated viable bacteria goods using or make through carrier adsorption.
5. a kind of complex microbial community according to claim 1 and 2 and enzyme product thereof, is characterized in that, the content range of the activeconstituents of each microbial bacteria of described microorganism species is 1 × 10
7cfu/g-1 × 10
8cfu/g, optimal way is, bacillus subtilis bacterial content is 6 × 10
7the content of cfu/g, bacillusmusilaginosiengineering is 2 × 10
7the content of cfu/g, pichia farinose is 5 × 10
7the content of cfu/g, bacillus amyloliquefaciens is 6 × 10
7the content of cfu/g, Pediococcus pentosaceus is 4 × 10
7the content of cfu/g, pediococcus acidilactici is 8 × 10
7cfu/g.
6. a kind of complex microbial community according to claim 1 and enzyme product thereof, is characterized in that, described microorganism species exists with the form of the fermented liquid of viable cell, viable cell by cultivating, the filtrate of cell culture or the mixture of cell and filtrate.
7. a kind of complex microbial community according to claim 1 and enzyme product thereof, is characterized in that, described complex microbial community and enzyme product thereof also comprise carrier, tensio-active agent, tackiness agent, stablizer and antioxidant, pH adjusting agent; Tensio-active agent is polysorbas20 or tween 80; Carrier be solid carrier and or liquid vehicle: described solid carrier can be mineral material, biomaterial and or macromolecular compound; Described mineral material is at least one in clay, talcum, kaolin, montmorillonite, white carbon, zeolite, silica, turfy soil and diatomite; Clay take attapulgite as the clay mineral of main component, and biomaterial is at least one in the ight soil of the stalk of all kinds of crop, loose shell, straw, Pericarppium arachidis hypogaeae, Semen Maydis powder, bean powder, starch, the peat composed of rotten mosses and animal; Macromolecular compound is polyvinyl alcohol and/or polyglycol; Described liquid vehicle is organic solvent, vegetables oil, mineral oil, clay or water; Described organic solvent is decane and or dodecane.
8. a kind of complex microbial community according to claim 1 and enzyme product thereof, it is characterized in that, described complex microbial community and enzyme product thereof are soil-repairing agent or microbial fertilizer, are applied to preparation and repair polluted soil and or improve soil and or increase soil fertility and or Promoting plant growth.
9. the preparation method of the pulvis of a kind of complex microbial community according to claim 1 and enzyme product thereof, it is characterized in that, step is as follows:
Q1: the activation of bacterium and enlarged culturing:
Activation: be seeded in respectively on substratum by each microbial bacteria of microorganism species, be placed in 28 DEG C of incubators and cultivate 5 days, obtains the microorganism species activated;
Enlarged culturing: the fermentor tank microorganism species of activation being accessed respectively substratum, all carries out static enlarged culturing step by step at 28 DEG C, and in enlarged culturing process, inoculum size is volume percent content 5%-10%; Obtain the fermented liquid 1 × 108cfu/g of microorganism species respectively;
Q2: the preparation of pulvis: by the fermented liquid of the microorganism species that Q1 step obtains, carries out being mixed to get fermentation mixed solution in colony forming units cfu number ratio; In fermentation mixed solution, the viable count of subtilis is 35% of total viable count, the viable count of bacillusmusilaginosiengineering is 15% of total viable count, the viable count of pichia farinose is 10% of total viable count, the viable count of bacillus amyloliquefaciens is 10% of total viable count, the viable count of Pediococcus pentosaceus is 15% of total viable count, the viable count of pediococcus acidilactici is 15% of total viable count.
10. mix according to weight ratio 2:3:2:2:1 with turfy soil, diatomite, Semen Maydis powder, starch, attapulgite clay, obtained microbial bacteria agent carrier; Q3: with phytase: proteolytic enzyme: cellulolytic enzyme: urase mixes according to weight ratio 4:1:3:2, add Q2 step the microbial bacteria agent carrier obtained and the mixed solution that ferments, keep total water content to be 70%, 30 DEG C of quiescent culture 3 days during mixing, 35 DEG C of naturally ventilated dryings obtain composite microbial bacteria enzyme system pulvis.The application method of pulvis in rehabilitating soil of a kind of complex microbial community according to claim 1 and enzyme product thereof, it is characterized in that, method steps is as follows:
Q1: be blended into by composite microbial bacteria enzyme system pulvis in warm water and leave standstill to activate medicament, the time is 12-24 hour;
Q2: the pulvis aqueous solution after activating is divided into 3 parts, a part evenly spray arrives soil table; Wholely, fertilising soil layer is ploughed under below earth's surface, to protect microbial inoculum not by excessive illumination effect;
Q3: on field planting same day, the pulvis aqueous solution after the activation of part 2 is evenly sprayed soil table; Wholely, fertilising soil layer is ploughed under below earth's surface, to protect microbial inoculum not by excessive illumination effect;
Q4: after field planting the 4th day, the 27th day, use the aqueous solution of the composite microbial bacteria enzyme system pulvis of dilution 200 times;
Q5: after field planting 29 days, the pulvis aqueous solution after the activation of the 3rd part is applied by plants stems stalk lower end mulch film opening part.
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