CN107619308A - Prevent and treat root-knot nematode biological organic fertilizer and preparation method and application - Google Patents
Prevent and treat root-knot nematode biological organic fertilizer and preparation method and application Download PDFInfo
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Abstract
The invention provides one kind preventing and treating root-knot nematode biological organic fertilizer and preparation method and application；The biological organic fertilizer includes 5 30 parts of composite bacteria agent, 70 95 parts of carrier；The composite bacteria agent contains bacillus subtilis, Paecilomyces lilacinus, thick wall spore Pu Keniya bacterium and streptomycete.The carrier is chicken manure, mushroom slag, rape cake form by the rotten fermentation of microorganism heap.The composite bacteria agent has the effect of root-knot nematode in preventing and treating soil, the organic principle and inorganic elements enriched in carrier not only can be with improved soil, the nutritional ingredient of raising soil, and composite bacteria agent effectively colonizing in soil, enhancing and control of the composite bacteria agent to root-knot nematode in soil can be accelerated.Preventing and treating root-knot nematode biological organic fertilizer provided by the invention is powdery, is used as base fertilizer, to person poultry harmless, prevention effect is good, can significantly improve the quality and yield of crop.
Description
Technical field
The present invention relates to organic fertilizer field, and in particular to one kind preventing and treating root-knot nematode biological organic fertilizer and preparation method thereof should
With.
Background technology
Root-knot nematode is the important monoid of plant nematode, is the main soil-borne disease to harm the crops.Its species is various,
Host range is extensive, has had a strong impact on the production of crops, has all brought massive losses to crops every year.Root-knot nematode is led to
Infection crops root is crossed, influences growing for crop, reduces crop quality, crop failure is caused or even has no harvest.This
Outside, root-knot nematode has been further exacerbated by the propagation of vegetative bacteria and fungal disease by the parasitization between plant.
The main method of preventing and treating plant root-knot nematode is chemical agent at present, throughout the year using chemical agent, is not only brought to agricultural product
The hidden danger of the food securities such as residues of pesticides, return a series of problems, such as environment brings water and soil pollution, ecological disruption.
The defects of in terms of based on traditional root knot nematode control, by combining the biological control feature of current popular, research and development one
The new bio-organic fertilizer for being used to prevent and treat meloidogyne of kind, by the way that the microorganism for preventing and treating root-knot nematode is added to
In organic fertilizer, production is a kind of can to provide nutrient for plant growth, and can prevents and treats the biological organic fertilizer caused harm of root-knot nematode
Product, it is the research emphasis that current many is directed to agricultural biological product development enterprise.
The content of the invention
It is an object of the invention to provide a kind of biological organic fertilizer that can prevent and treat root-knot nematode in soil, to reduce agricultural chemicals and change
Fertilizer is applied, and is promoted the growth of crop and is improved crop yield.The present invention also provides the preventing and treating root-knot nematode biological organic fertilizer
Preparation method and application.
In order to realize the object of the invention, a kind of preventing and treating root-knot nematode biological organic fertilizer of the invention includes composite bacteria agent 5-30
Parts by weight, carrier 70-95 parts by weight；Wherein, bacillus subtilis (Bacillus subtilis) is contained in the composite bacteria agent
The cfu/g of 5-25 hundred million, the cfu/g of Paecilomyces lilacinus (Paecilomyces lilacinus) 0.5-5.0 hundred million, thick wall spore Pu Keniya bacterium
The cfu/g of (Pochonia chlamydosporia) 0.5-3.0 hundred million and cfu/g of streptomycete (Streptomycetaceae) 0.5-3.0 hundred million；
The carrier is chicken manure, mushroom slag, rape cake form by the rotten fermentation of microorganism heap, is preferably handled through drying and screening, is powder
Shape thing.
Preferably, the preventing and treating root-knot nematode biological organic fertilizer includes composite bacteria agent 10-25 parts by weight, carrier 75-90 weights
Measure part；Wherein, the cfu/g of bacillus subtilis 10-20 hundred million, Paecilomyces lilacinus 1.0-4.0 hundred million are contained in the composite bacteria agent
Cfu/g, the thick cfu/g of the wall spore Pu Keniya bacterium 1.0-2.5 hundred million and cfu/g of streptomycete 1.0-2.5 hundred million；The carrier be chicken manure,
Mushroom slag, rape cake form by the rotten fermentation of microorganism heap, are preferably handled through drying and screening, are powder.
It is highly preferred that the preventing and treating root-knot nematode biological organic fertilizer includes the parts by weight of composite bacteria agent 20, the parts by weight of carrier 80；
Contain the cfu/g of bacillus subtilis 1,500,000,000, the cfu/g of Paecilomyces lilacinus 300,000,000, thick wall spore Pu Keni in the composite bacteria agent
The sub- cfu/g of the bacterium 200,000,000 and cfu/g of streptomycete 200,000,000；The carrier is chicken manure, mushroom slag, rape cake by the rotten hair of microorganism heap
Ferment forms, and is preferably handled through drying and screening, is powder.
Aforementioned bearer is mixed in proportion by chicken manure, mushroom slag, rape cake, is formed by the rotten fermentation of microorganism heap；Wherein,
Preferably, microorganism (fermenting agent) needed for the rotten fermentation of the heap is bacillus subtilis and Trichoderma viride；It is further excellent
Selection of land, bacillus subtilis total bacteria count is 20,000,000,000 cfu/g in the leavening, and Trichoderma viride total bacteria count is 500,000,000 cfu/g,
Addition is 2.0kg/ ton dry materials；Preferably, the chicken manure, mushroom slag, the mass ratio of rape cake are 1:1:1.
Specifically, the preparation method of aforementioned bearer includes：
By chicken manure, mushroom slag, rape cake by proportioning mix, then add fermenting agent, be well mixed, regulation moisture to
50%-60%, the high 1.0-1.5 rice of heap, stack retting 20-30 days；Cover film when fermenting initial, treat that fermentation heap central temperature reaches
Film is removed during to more than 50 DEG C, is fitted when fermentation heap central temperature is more than 65 DEG C with turning machine turning, 10 days before fermentation
Work as moisturizing so that fermentation heap moisture maintains more than 50%；So operate repeatedly, treat that fermentation heap color becomes coffee-like or black,
Fermentation heap temperature is down to less than 35 DEG C simultaneously, terminates to ferment when water content is below 38%, the material fermented is dried,
Broken, sieving, produces carrier.
Preferably, preventing and treating root-knot nematode biological organic fertilizer of the present invention is pulvis.
Parts by weight of the present invention can be the unit of weight well known in the art such as μ g, mg, g, kg or its
Multiple, such as 1/10,1/100,10 times, 100 times.
The preparation method of the composite bacteria agent, comprises the following steps：
I. the preparation of bacillus subtilis bacterium powder：Bacillus subtilis original strain is aseptically subjected to inclined-plane successively
After culture, shaking table culture, fermentation tank culture, obtained zymotic fluid is mixed with cornstarch, prepared by being concentrated and dried
Into Bacillus globigii spores powder；
II. the preparation of Paecilomyces lilacinus conidia powder：Paecilomyces lilacinus strain is aseptically subjected to inclined-plane training successively respectively
Support, after shaking table culture, fermentation tank culture, solid fermentation production spore, obtained solid fermentation product is concentrated and dried and is prepared into
Paecilomyces lilacinus conidia powder；
III. the preparation of thick wall spore Pu Keniya bacterium conidia powders：By thick wall spore Pu Keniya bacterium original strain aseptically according to
It is after secondary progress inclined-plane culture, shaking table culture, fermentation tank culture, solid fermentation production spore, obtained solid fermentation product is dense
Contracting drying is prepared into thick wall spore Pu Keniya bacterium conidia powders；
IV. the preparation of streptomycete bacterium powder：Streptomycete original strain is aseptically carried out to inclined-plane culture, shaking table training successively
Support, after fermentation tank culture, obtained zymotic fluid is mixed with cornstarch, streptomycete bacterium powder is prepared into by concentrate drying；
V. by bacillus subtilis bacterium powder obtained above, Paecilomyces lilacinus conidia powder, thick wall spore Pu Keniya bacterium conidia powder,
Streptomycete bacterium powder mixes in proportion, produces composite bacteria agent.
Preparation process I, II, III, IV of above-mentioned composite bacteria agent are specifically included：
I. the preparation of bacillus subtilis mixing conidia powder：
A. inclined-plane culture：Bacillus subtilis original strain is aseptically inoculated on slant medium,
36-48 hours are cultivated under the conditions of 35 ± 2 DEG C；
B. shaking table culture：The strain that step a is cultivated aseptically is inoculated in seed culture medium,
PH6.5-7.0, under the conditions of 35 DEG C, 140-160r/min shaking table culture 12-18 hours；
C. fermentation tank culture：The strain that step b is cultivated aseptically is inoculated in liquid fermentation medium,
PH7.5-8.0, tank pressure 0.5kg, 35 DEG C, ventilation 1:Under the conditions of 0.8-1.1, after cultivating 48-56 hours, viable count is big
In 1.0 × 109Cfu/mL, 80% thalline turn into gemma tank at present, obtain zymotic fluid；
D. by the zymotic fluid obtained in step c by etc. weight than mixing cornstarch, be prepared into withered grass bud by concentrate drying
Spore bacillus mixes conidia powder；
Wherein, the formula of the slant medium used in step a is as follows：Glucose 15g, fish peptone 5g, yeast extract
5g, agar 15g and water 1000mL；The seed culture based formulas used in step b is as follows：Glucose 10g, beef
Cream 5g, dusty yeast 5g, starch 10g, beancake powder 5g, KH2PO4 0.5g、MgSO40.2g and water 1000mL；
The liquid fermentation medium formula used in step c is as follows：Corn flour 26kg, beancake powder 16kg, ammonium sulfate 4kg,
Glucose 8kg, dusty yeast 2.5kg, peptone 1.7kg and defoamer 50mL, add water to 600L；
II. the preparation of Paecilomyces lilacinus conidia powder：
E. Paecilomyces lilacinus original strain is aseptically inoculated on slant medium, cultivated under the conditions of 28 DEG C
72 hours；
F. shaking table culture：The strain that step e is cultivated aseptically is inoculated in seed culture medium,
PH6.0-6.5, under the conditions of 26 DEG C, 160r/min shaking table cultures 24 hours；
G. fermentation tank culture：The step f strains cultivated aseptically are inoculated in liquid fermentation medium respectively,
In pH6.0-7.0, tank pressure 0.5kg, 26 DEG C, ventilation 1:0.6-0.8, after culture 72 hours, mycelium accounts for always
Volume 20% when terminate fermentation, carry out solid fermentation production spore；
H. solid fermentation production spore：Mycelium of the step g after fermentation tank culture is inoculated on solid fermentation culture medium,
168-180 hours are cultivated, stop fermentation when Paecilomyces lilacinus 90% produces spore；
I. the solid fermentation product obtained in step h is concentrated and dried and is prepared into Paecilomyces lilacinus conidia powder；
Wherein, the formula of the slant medium used in step e is as follows：Glucose 20g, murphy juice 200g, agar 20g
It is natural with water 1000mL, pH；The seed culture based formulas used in step f is as follows：White sugar 20g, peptone 5g,
Potassium dihydrogen phosphate 0.2g, magnesium sulfate 0.2g, sodium chloride 2g and water 1000mL；The liquid fermentation training used in step g
It is as follows to support based formulas：Starch 12kg, corn flour 3kg, beancake powder 1.2kg, white sugar 12kg, peptone 3kg, sulfuric acid
Magnesium 0.12kg, sodium chloride 12kg, defoamer 50mL, add water to 600L；Solid fermentation culture medium in step h is matched somebody with somebody
Side is as follows：Wheat bran, corn flour and beancake powder press 7:2:1 weight adds water in the solid material than mixing composition, produces solid
The weight ratio of fermentation medium, solid material and water is 1:0.6；
III. the preparation of thick wall spore Pu Keniya bacterium conidia powders：
J. aseptically, thick wall spore Pu Keniya bacterium strains are inoculated on slant medium, cultivated at 28 DEG C
5-7 days；
K. shaking table culture：The strain that step j is cultivated aseptically is inoculated in seed culture medium, in 28 DEG C of conditions
Under, 160r/min shaking table cultures 72 hours；
L. fermentation tank culture：Aseptically, in strain access liquid fermentation medium step k cultivated, first
Beginning pH7.0-8.0, tank pressure 0.5kg, 28 DEG C, ventilation 1:0.5-1,48-60 hours are cultivated, terminate fermentation, consolidate
Body fermentation production spore；
M. solid fermentation production spore：Mycelium of the step l after fermentation tank culture is inoculated on solid fermentation culture medium,
Culture 8-10 days, stop fermentation when thick wall spore Pu Keniya bacterium 90% produce spore；
N. the solid fermentation product obtained in m is concentrated and dried and is prepared into thick wall spore Pu Keniya bacterium conidia powders；
Wherein, the formula of the slant medium used in step j is as follows：Sucrose 20g, murphy juice 200g, agar 20g
It is natural with water 1000mL, pH；The seed culture based formulas used in step k is as follows：Sucrose 30g, sodium nitrate 5g,
Potassium dihydrogen phosphate 1g, magnesium sulfate 0.5g, potassium chloride 0.5g, ferrous sulfate 0.02g and water 1000mL；Make in step l
Liquid fermentation medium formula is as follows：Corn flour 12kg, soy meal 3kg, starch 1.2kg, sucrose 30g, nitric acid
Sodium 5g, potassium dihydrogen phosphate 1g, magnesium sulfate 0.5g, potassium chloride 0.5g, ferrous sulfate 0.02g defoamer 50mL, add
Water is to 600L；Solid fermentation culture medium prescription in step m is as follows：Corn flour, soy meal and wheat bran press 7:2:1 weight
Than the solid material of mixing composition, 2% sucrose, 1% sodium nitrate, 1% sodium chloride are re-added by material, adds water to mix thoroughly, produces
The weight ratio of solid fermentation culture medium, solid material and water is 1:0.5.
IV. the preparation of streptomycete bacterium powder：
O. aseptically, streptomyces species are inoculated on slant medium, cultivated 5-6 days at 28 DEG C；
P. shaking table culture：The strain that step o is cultivated aseptically is inoculated in seed culture medium, in 28 DEG C of conditions
Under, 220r/min shaking table cultures 24 hours；
Q. fermentation tank culture：Aseptically, in strain access liquid fermentation medium step p cultivated, first
Beginning pH7.5, tank pressure 0.5kg, 28 DEG C, ventilation 1:0.5-1, after cultivating 48 hours, put tank and collect zymotic fluid, add jade
Conidia powder is made after the concentrated drying of rice starch；
Wherein, the formula of the slant medium used in step o is as follows：Soluble starch 20g, KNO31g、NaCl0.5g、
K2HPO40.5g、MgSO40.5g、FeSO40.1g, agar powder 18g and distilled water 1000mL, pH7.2-7.4；Step
The culture medium prescription used in rapid p, q is as follows：Cornstarch 20g, bean powder 10g, NaCl0.5g, K2HPO40.5g、
(NH4)2SO45g、CaCO33g, defoamer 0.5g and distilled water 1000mL, pH7.5.
The present invention also provides the preparation method of above-mentioned preventing and treating root-knot nematode biological organic fertilizer, comprises the following steps：
(1) preparation of carrier
(2) preparation of composite bacteria agent
(3) preparation of root-knot nematode biological organic fertilizer is prevented and treated：The carrier is mixed in proportion with composite bacteria agent, i.e.,
Root-knot nematode biological organic fertilizer must be prevented and treated.
The deposit number of foregoing bacillus subtilis is ACCC 11025, the deposit number of Paecilomyces lilacinus is ACCC
30673rd, the deposit number of thick wall spore Pu Keniya bacterium be ACCC 30601, the deposit number of streptomycete be ACCC
40460；It can be bought by ACCC.
ACCC represents Chinese agriculture Microbiological Culture Collection administrative center.
Mushroom slag of the present invention refers to remaining solid waste after various edible fungus culturings, commercially available to buy.
The present invention also provides application of the preventing and treating root-knot nematode biological organic fertilizer in crop-planting.
The preventing and treating root-knot nematode biological organic fertilizer method：
1. Special basal fertilizer, every mu of dosage 100-300kg can be used as；Also auxiliary base manure can be used as to use, every mu of dosage
20-40kg, can ditch spread, spread manuer in holes, ground is spread fertilizer over the fields；
2. particularly suitable make base manure；
3. using to be poured water in time after this product, fertilizer efficiency is more preferably；
4. using timely earthing or arable land is wanted after this product, this product is not set to be exposed under sunlight；
5. forbid directly to be used in mixed way with bactericide；
6. deposit at shady and cool dry.
The preventing and treating root-knot nematode biological organic fertilizer has water suction moisturizing, increases soil fertility, assists crop to absorb nutrition,
And have preventing and treating meloidogyne, crop disease prevention ability is improved, the effect of disease occurs is reduced, is remarkably improved and makees article
Matter and yield.
The present invention has advantages below：
(1) in preventing and treating root-knot nematode biological organic fertilizer of the invention, microbe species collocation is reasonable, Paecilomyces lilacinus
There is very strong prevention effect to the root-knot nematode in soil with thick wall spore Pu Keniya bacterium, streptomycete also there is certain desinsection to live
Property, bacillus subtilis can colonize rapidly to form dominant strain in crop root, and its metabolite is to the cause in soil
Germ has obvious inhibitory action.These microorganisms and organic fertilizer are reasonably combined, can improve well caused by continuous cropping plantation
The situation that soil environment deteriorates.In addition, carried present invention employs the chicken manure after fermentation maturity, mushroom slag, rape cake to be main
Body, to discarded object carried out recovery and effectively utilize, good economic benefit can be produced, by microbial fermentation it is decomposed after
The nitrogen, phosphorus, potassium of plant growth needs can be provided, the needed nutrient matter that can guarantee that plant growth is applied as base manure, it is long
Phase use can improve soil quality well.
(2) effective advanced science of bacterium preparation method of the invention, simple and feasible, spore count content is high, the time-to-live
It is long, it can quickly be colonized in soil, form dominant colony, promote the healthy growth of crop.
Embodiment
Following examples are not limited to the scope of the present invention to for illustrating the present invention.Unless otherwise specified, implement
The conventional meanses that technological means used is well known to those skilled in the art in example, raw materials used is commercial goods.
Mushroom slag described in following examples is purchased from Hunan Hua Tiansheng morals bio tech ltd.
Embodiment 1 prevents and treats root-knot nematode biological organic fertilizer and preparation method thereof
Prevent and treat raw material of the root-knot nematode biological organic fertilizer containing following parts by weight meter
80 parts of carrier；
20 parts of composite bacteria agent.
Effective viable bacteria total content is 2,200,000,000 cfu/g in the composite bacteria agent, is formed as follows：
The carrier is chicken manure, mushroom slag, rape cake by the rotten fermentation of microorganism heap, the powder of drying and screening processing.
The preparation method of above-mentioned preventing and treating root-knot nematode biological organic fertilizer, comprises the following steps：
(1) preparation of carrier
By chicken manure, mushroom slag, rape cake mass ratio 1 in parts by weight:1:1 mixes, and then adds fermenting agent, is well mixed,
Moisture is adjusted to 50%-60%, the high 1.0-1.5 rice of heap, stack retting 20-30 days；Bacillus subtilis in the fermenting agent
Total bacteria count is 20,000,000,000 cfu/g, and Trichoderma viride total bacteria count is 500,000,000 cfu/g, and addition is 2.0kg/ ton dry materials.
Cover film when fermenting initial, film is removed when fermentation heap central temperature reaches more than 50 DEG C, when fermentation heap center
With turning machine turning when temperature is more than 65 DEG C, appropriate moisturizing in 10 days before fermentation so that fermentation heap moisture maintains more than 50%；
So operate repeatedly, treat that fermentation heap color becomes coffee-like or black, while fermentation heap temperature is down to less than 35 DEG C, water contains
Terminate to ferment when amount is below 38%, by the material fermented drying, broken, sieving, produce carrier.
(2) preparation of composite bacteria agent
I. the preparation of bacillus subtilis bacterium powder：Bacillus subtilis original strain is aseptically subjected to inclined-plane successively
After culture, shaking table culture, fermentation tank culture, obtained zymotic fluid is mixed with cornstarch, prepared by being concentrated and dried
Into Bacillus globigii spores powder；
II. the preparation of Paecilomyces lilacinus conidia powder：Paecilomyces lilacinus strain is aseptically subjected to inclined-plane training successively respectively
Support, after shaking table culture, fermentation tank culture, solid fermentation production spore, obtained solid fermentation product is concentrated and dried and is prepared into
Paecilomyces lilacinus conidia powder；
III. the preparation of thick wall spore Pu Keniya bacterium conidia powders：By thick wall spore Pu Keniya bacterium original strain aseptically according to
It is after secondary progress inclined-plane culture, shaking table culture, fermentation tank culture, solid fermentation production spore, obtained solid fermentation product is dense
Contracting drying is prepared into thick wall spore Pu Keniya bacterium conidia powders；
IV. the preparation of streptomycete bacterium powder：Streptomycete original strain is aseptically carried out to inclined-plane culture, shaking table training successively
Support, after fermentation tank culture, obtained zymotic fluid is mixed with cornstarch, streptomycete bacterium powder is prepared into by concentrate drying；
V. by bacillus subtilis bacterium powder obtained above, Paecilomyces lilacinus conidia powder, thick wall spore Pu Keniya bacterium conidia powder,
Streptomycete bacterium powder mixes in proportion, produces composite bacteria agent.
(3) preparation of root-knot nematode biological organic fertilizer is prevented and treated：
The carrier of gained is mixed in proportion with composite bacteria agent, that is, prevents and treats root-knot nematode biological organic fertilizer.
Step I, II, III, IV are specially：
I. the preparation of bacillus subtilis mixing conidia powder：
A. inclined-plane culture：Bacillus subtilis original strain is aseptically inoculated on slant medium,
36-48 hours are cultivated under the conditions of 35 ± 2 DEG C；
B. shaking table culture：The strain that step a is cultivated aseptically is inoculated in seed culture medium,
PH6.5-7.0, under the conditions of 35 DEG C, 140-160r/min shaking table culture 12-18 hours；
C. fermentation tank culture：The strain that step b is cultivated aseptically is inoculated in liquid fermentation medium,
PH7.5-8.0, tank pressure 0.5kg, 35 DEG C, ventilation 1:Under the conditions of 0.8-1.1, after cultivating 48-56 hours, viable count is big
In 1.0 × 109Cfu/mL, 80% thalline turn into gemma tank at present, obtain zymotic fluid；
D. by the zymotic fluid obtained in step c by etc. weight than mixing cornstarch, be prepared into withered grass bud by concentrate drying
Spore bacillus mixes conidia powder；
Wherein, the formula of the slant medium used in step a is as follows：Glucose 15g, fish peptone 5g, yeast extract
5g, agar 15g and water 1000mL；The seed culture based formulas used in step b is as follows：Glucose 10g, beef
Cream 5g, dusty yeast 5g, starch 10g, beancake powder 5g, KH2PO4 0.5g、MgSO40.2g and water 1000mL；
The liquid fermentation medium formula used in step c is as follows：Corn flour 26kg, beancake powder 16kg, ammonium sulfate 4kg,
Glucose 8kg, dusty yeast 2.5kg, peptone 1.7kg and defoamer 50mL, add water to 600L；
II. the preparation of Paecilomyces lilacinus conidia powder：
E. Paecilomyces lilacinus original strain is aseptically inoculated on slant medium, cultivated under the conditions of 28 DEG C
72 hours；
F. shaking table culture：The strain that step e is cultivated aseptically is inoculated in seed culture medium,
PH6.0-6.5, under the conditions of 26 DEG C, 160r/min shaking table cultures 24 hours；
G. fermentation tank culture：The step f strains cultivated aseptically are inoculated in liquid fermentation medium respectively,
In pH6.0-7.0, tank pressure 0.5kg, 26 DEG C, ventilation 1:0.6-0.8, after culture 72 hours, mycelium accounts for always
Volume 20% when terminate fermentation, carry out solid fermentation production spore；
H. solid fermentation production spore：Mycelium of the step g after fermentation tank culture is inoculated on solid fermentation culture medium,
168-180 hours are cultivated, stop fermentation when Paecilomyces lilacinus 90% produces spore；
I. the solid fermentation product obtained in step h is concentrated and dried and is prepared into Paecilomyces lilacinus conidia powder；
Wherein, the formula of the slant medium used in step e is as follows：Glucose 20g, murphy juice 200g, agar 20g
It is natural with water 1000mL, pH；The seed culture based formulas used in step f is as follows：White sugar 20g, peptone 5g,
Potassium dihydrogen phosphate 0.2g, magnesium sulfate 0.2g, sodium chloride 2g and water 1000mL；The liquid fermentation training used in step g
It is as follows to support based formulas：Starch 12kg, corn flour 3kg, beancake powder 1.2kg, white sugar 12kg, peptone 3kg, sulfuric acid
Magnesium 0.12kg, sodium chloride 12kg, defoamer 50mL, add water to 600L；Solid fermentation culture medium in step h is matched somebody with somebody
Side is as follows：Wheat bran, corn flour and beancake powder press 7:2:1 weight adds water in the solid material than mixing composition, produces solid
The weight ratio of fermentation medium, solid material and water is 1:0.6；
III. the preparation of thick wall spore Pu Keniya bacterium conidia powders：
J. aseptically, thick wall spore Pu Keniya bacterium strains are inoculated on slant medium, cultivated at 28 DEG C
5-7 days；
K. shaking table culture：The strain that step j is cultivated aseptically is inoculated in seed culture medium, in 28 DEG C of conditions
Under, 160r/min shaking table cultures 72 hours；
L. fermentation tank culture：Aseptically, in strain access liquid fermentation medium step k cultivated, first
Beginning pH7.0-8.0, tank pressure 0.5kg, 28 DEG C, ventilation 1:0.5-1,48-60 hours are cultivated, terminate fermentation, consolidate
Body fermentation production spore；
M. solid fermentation production spore：Mycelium of the step l after fermentation tank culture is inoculated on solid fermentation culture medium,
Culture 8-10 days, stop fermentation when thick wall spore Pu Keniya bacterium 90% produce spore；
N. the solid fermentation product obtained in m is concentrated and dried and is prepared into thick wall spore Pu Keniya bacterium conidia powders；
Wherein, the formula of the slant medium used in step j is as follows：Sucrose 20g, murphy juice 200g, agar 20g
It is natural with water 1000mL, pH；The seed culture based formulas used in step k is as follows：Sucrose 30g, sodium nitrate 5g,
Potassium dihydrogen phosphate 1g, magnesium sulfate 0.5g, potassium chloride 0.5g, ferrous sulfate 0.02g and water 1000mL；Make in step l
Liquid fermentation medium formula is as follows：Corn flour 12kg, soy meal 3kg, starch 1.2kg, sucrose 30g, nitric acid
Sodium 5g, potassium dihydrogen phosphate 1g, magnesium sulfate 0.5g, potassium chloride 0.5g, ferrous sulfate 0.02g defoamer 50mL, add
Water is to 600L；Solid fermentation culture medium prescription in step m is as follows：Corn flour, soy meal and wheat bran press 7:2:1 weight
Than the solid material of mixing composition, 2% sucrose, 1% sodium nitrate, 1% sodium chloride are re-added by material, adds water to mix thoroughly, produces
The weight ratio of solid fermentation culture medium, solid material and water is 1:0.5.
IV. the preparation of streptomycete bacterium powder：
O. aseptically, streptomyces species are inoculated on slant medium, cultivated 5-6 days at 28 DEG C；
P. shaking table culture：The strain that step o is cultivated aseptically is inoculated in seed culture medium, in 28 DEG C of conditions
Under, 220r/min shaking table cultures 24 hours；
Q. fermentation tank culture：Aseptically, in strain access liquid fermentation medium step p cultivated, first
Beginning pH7.5, tank pressure 0.5kg, 28 DEG C, ventilation 1:0.5-1, after cultivating 48 hours, put tank and collect zymotic fluid, add jade
Conidia powder is made after the concentrated drying of rice starch；
Wherein, the formula of the slant medium used in step o is as follows：Soluble starch 20g, KNO31g、NaCl0.5g、
K2HPO40.5g、MgSO40.5g、FeSO40.1g, agar powder 18g and distilled water 1000mL, pH7.2-7.4；Step
The culture medium prescription used in rapid p, q is as follows：Cornstarch 20g, bean powder 10g, NaCl0.5g, K2HPO40.5g、
(NH4)2SO45g、CaCO33g, defoamer 0.5g and distilled water 1000mL, pH7.5.
Embodiment 2 prevents and treats root-knot nematode biological organic fertilizer and preparation method thereof
Prevent and treat raw material of the root-knot nematode biological organic fertilizer containing following parts by weight meter
75 parts of carrier；
25 parts of composite bacteria agent.
Effective viable bacteria total content is 2,900,000,000 cfu/g in the composite bacteria agent, is formed as follows：
The carrier is chicken manure, mushroom slag, rape cake by the rotten fermentation of microorganism heap, the powder of drying and screening processing, system
Preparation Method is the same as implementation column 1.
The preparation method of the preventing and treating root-knot nematode biological organic fertilizer is the same as implementation column 1.
Embodiment 3 prevents and treats root-knot nematode biological organic fertilizer and preparation method thereof
Prevent and treat raw material of the root-knot nematode biological organic fertilizer containing following parts by weight meter
90 parts of carrier；
10 parts of composite bacteria agent.
Effective viable bacteria total content is 1,400,000,000 cfu/g in the composite bacteria agent, is formed as follows：
The carrier is chicken manure, mushroom slag, rape cake by the rotten fermentation of microorganism heap, the powder of drying and screening processing, system
Preparation Method is the same as implementation column 1.The preparation method of the preventing and treating root-knot nematode biological organic fertilizer is the same as implementation column 1.
Embodiment 4 prevents and treats root-knot nematode biological organic fertilizer and preparation method thereof
Prevent and treat raw material of the root-knot nematode biological organic fertilizer containing following parts by weight meter
70 parts of carrier；
30 parts of composite bacteria agent.
Effective viable bacteria total content is 3,600,000,000 cfu/g in the composite bacteria agent, is formed as follows：
The carrier is chicken manure, mushroom slag, rape cake by the rotten fermentation of microorganism heap, the powder of drying and screening processing, system
Preparation Method is the same as implementation column 1.The preparation method of the preventing and treating root-knot nematode biological organic fertilizer is the same as implementation column 1.
Experimental example 1 prevents and treats root-knot nematode biological organic fertilizer field test
1. test objective：
Verify preventing and treating root-knot nematode biological organic fertilizer preventing and treating meloidogyne, improve crop yield and control nematode propagation
The effect of disease.
2. test material：
Plant：For the examination big Fructus Lycopersici esculenti of tomato variety (commercially available).
Fertilizer：The preventing and treating root-knot nematode biological organic fertilizer prepared in embodiment 1；Commercially available " Wei Hong " melon and fruit class is special organic
Fertile (being purchased from plentifulness bio tech ltd of Ningxiang), day, which reach, carries seed manure (reaching Biological Co., Ltd. purchased from Shandong day)；
Carbon ammonium, calcium superphosphate, potassium sulfate etc..
Testing site:Positioned at Liuyang City of Hunan Province Bei Sheng towns proving ground, 1 is shown in Table for the examination main agrochemical characteristic of soil, preceding stubble is made
Thing is rape.
3. experimental design
3.1 with reference to the field in microbial manure field experiment technical regulation and fertilizer efficiency evaluation guide (NY/T 1536-2007)
Between effect experiment design requirement.5 groups of experiment point, every group of cultivated area 30m2, every group is done 3 repetitions and tested.Plantation
Density is 4000 plants/acre, and insect pest preventing and controlling etc. are managed by Production of Large Fields requirement.
Experimental group A：Using the preventing and treating root-knot nematode biological organic fertilizer prepared in embodiment 1, every mu of dosage 100kg.
The preventing and treating root-knot nematode biological organic fertilizer prepared in Example 1 is by every mu of dosage 50kg.Appropriate base manure is added (" to defend
It is red " melon and fruit class fertilizer special for organic 100kg/ mus, carbon ammonium 12kg/ mus, calcium superphosphate 30kg/ mus, potassium sulfate 15kg/ mus)
Base manure administration is done in mixing；Biological organic fertilizer prepared by another Example 1 is same with other top dressings respectively by every mu of dosage 50kg
Apply, (tomato was colonized in one week, imposed once day up to carrying seed manure for other conventional fertilizer application amounts；First fringe fruit stone peach combines when big
Watering mu applies urea 6kg, potassium sulfate 10kg；Second and third, four fringe fruit expanding stages are when starting, each top dressing 1 time, mu is applied
Urea 5kg；Into fruiting period, the potassium dihydrogen phosphate of foliage-spray 0.5%) normally apply.
Experimental group B：Using the preventing and treating root-knot nematode biological organic fertilizer prepared in embodiment 1, every mu of dosage 100kg.
The preventing and treating root-knot nematode biological organic fertilizer prepared in Example 1 is applied by every mu of dosage 50kg as base manure；Separately
Biological organic fertilizer prepared by Example 1 does top dressing use by every mu of dosage 50kg, and other doses are with reference to experimental group A
The 80% of dose is applied.
Experimental group C:Except the preventing and treating root-knot nematode biological organic fertilizer sterilization treatment that will be prepared in embodiment 1, every mu of usage amount
Outside 100kg, dose and fertilizing method are the same as experimental group A.
Experimental group D:Only conventional fertilizer application, carried out with reference to experimental group A.
Experimental group E:Any fertilizer (blank control) is not applied.
3.2 experiments are implemented：
Performed by 3.1, and carry out field management, record, analysis and meter production work.
3.2.1 field management：
Each test group sowing, fertilising, watering, weeding, the prevention and control of plant diseases, pest control were completed in 1 day, and human error is reduced
To minimum degree.
A, fertilizing management：Carry out by local Fertilization Level and fertilizing method, according to weather and soil moisture situation, open up
Irrigation canals and ditches, carry out prevention waterlogging drought resisting and prepare；
B, the prevention and control of plant diseases, pest control：Rely mainly on prevention, carry out the unified processing that carried out disinfection to seed before sowing, strengthen cultivation management,
It was found that the state of an illness removes diseased plant in time.
3.2.2 test data sheet
A, for the kind of examination tomato；
B, test site, test period, method design, plot area, cell arrangement, number of repetition (use are indicated
The form of chart)；
C, experimental field landform, the soil texture, soil types, preceding crop species；
D, fertilization time, method, quantity and number etc.；
E, precipitation and irrigation quantity during testing；
F, prevention and control of plant diseases, pest control situation and other farming activities etc.；
G, the upgrowth situation field investigation of crop, including survival rate of transplanting seedlings, growing way, breeding time.And a situation arises for disease pest
Deng.
3.2.3 harvest and count production
3.2.4 the record such as crop economical character, soil fertility and disease generation
3.3 effect assessment
Crop prevention effect and yield comparison situation are shown in Table 2.
Take 10 plants of progress root knot condition surveys at random per cell when tomato is transplanted 60 days.Disease index, which calculates, uses 0~4
The grade scale of level carries out severity Scaling, counts disease index, and calculate preventive effect.With reference to Xiao Yannong etc. grade scale：
0 grade, no root knot；1 grade, the root system for having root knot accounts for the 1%~24% of full root system；2 grades, the root system for having root knot accounts for full root system
25%~49%；3 grades, the root system for having root knot accounts for the 50%~74% of full root system；4 grades, the root system for having root knot accounts for full root
The 75%~100% of system.
Prevention effect=[(disease index after blank control disease index-fertilizer treatment)/blank control disease index] × 100%
The volume variance prevent and treat between the processing of root-knot nematode biological organic fertilizer and other each processing is analyzed.Increase production significant difference
Horizontal experiment points reach more than 2/3 person of sum, judge that the product has effect of increasing production.
3.3.4 resistance effect assessment
Resistance includes suppressing pest and disease damage generation (state of an illness and incidence of disease record), drought resisting, overcomes continuous cropping obstacle etc..
Resistance index, which compares, correlates the positive effect for improving more than 20%.Tomato state of an illness situation is shown in Table 3.
3.3.4 reclamation result is evaluated
Determine the indexs such as microbial population and quantity, organic matter, rapid available phosphorus, available potassium, available nitrogen, the pH in soil.
Soil-like is taken before minute is applied for transplanting followed by monthly to be once measured until tomato harvesting terminates.
3.3.5 safety index is evaluated
Trial crops and soil are carried out with the measure of the poisonous and harmful substance contents such as residues of pesticides, heavy metal, it is anti-to evaluate
Control whether root-knot nematode biological organic fertilizer has degraded and transformation function to it.
Appendix A (normative appendix)
Matrix steriling test method
A.1 sample
The grab sample from matrix sample.
A.2 sample survey
A.2.1 culture medium is prepared
Tetra- kinds of culture mediums of A1, A9, A11, A13 are prepared according to the requirement in NY/T 1114-2006.
A.2.2 the preparation of bacteria suspension
Sample 10g (being accurate to 0.01g) is weighed, is added in the sterilized water of the 100mL with bead, in 200r/min
Fully vibration 30min, is made bacteria suspension in shaking table.
A.2.3 it is loaded and cultivates
0.1mL bacteria suspensions are taken to be added separately on four kinds of solid medium flat boards preparing, and with sterile spreading rod by bacterium
Suspension is equably coated on media surface, if 3 repetitions, 2d-7d is cultivated under appropriate temperature conditions.And with sterile
Water makees blank control.
A.2.4 sterilization effect is identified
Blank control is sterile to be dropped out now, and total plate count≤5 on other culture plates, then the sample can be used as matrix examination
Test.Conversely, it must sterilize again.Blank control has bacterium colony appearance, must reform steriling test.
4. result of the test
Experiment is carried out in strict accordance with testing program, and without seedling death phenomenon after transplanting, it is equal in addition to test group E processing to survive rear growing way
Act normally.
Main agrochemical feature of the table 1 for examination soil
Table 2 is respectively handled to tomato root-knot eelworm disease index, the influence of yield
The influence that respectively tomato crop field disease occurs for processing of table 3
Although above the present invention is described in detail with a general description of the specific embodiments, at this
On the basis of invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, belong to claimed model without departing from theon the basis of the spirit of the present invention
Enclose.
Claims (10)
1. one kind preventing and treating root-knot nematode biological organic fertilizer, it is characterised in that including composite bacteria agent 5-30 parts by weight, carrier
70-95 parts by weight；Wherein, the composite bacteria agent contains the cfu/g of bacillus subtilis 5-25 hundred million, Paecilomyces lilacinus 0.5-5.0
Hundred million cfu/g, the thick cfu/g of the wall spore Pu Keniya bacterium 0.5-3.0 hundred million and cfu/g of streptomycete 0.5-3.0 hundred million；The carrier be chicken manure,
The powder of mushroom slag, rape cake by the rotten fermentation of microorganism heap into drying and screening processing.
2. preventing and treating root-knot nematode biological organic fertilizer according to claim 1, it is characterised in that including composite bacteria agent
10-25 parts by weight, carrier 75-90 parts by weight；Wherein, the composite bacteria agent contain the cfu/g of bacillus subtilis 10-20 hundred million,
The cfu/g of Paecilomyces lilacinus 1.0-4.0 hundred million, the thick cfu/g of the wall spore Pu Keniya bacterium 1.0-2.5 hundred million and cfu/g of streptomycete 1.0-2.5 hundred million.
3. preventing and treating root-knot nematode biological organic fertilizer according to claim 1, it is characterised in that including composite bacteria agent
20 parts by weight, the parts by weight of carrier 80；Wherein, the composite bacteria agent contains the cfu/g of bacillus subtilis 1,500,000,000, pale purple
The cfu/g of Paecilomyces varioti 300,000,000, the thick cfu/g of wall spore Pu Keniya bacterium 200,000,000 and the cfu/g of streptomycete 200,000,000.
4. the preventing and treating root-knot nematode biological organic fertilizer according to claim any one of 1-3, it is characterised in that described
Carrier is by chicken manure, mushroom slag, rape cake example 1 in mass ratio:1:1 mixing, formed by the rotten fermentation of fermenting agent heap；Its
In, it is preferable that the fermenting agent is bacillus subtilis and Trichoderma viride mixed fermenting agent；It is further preferred that
Bacillus subtilis total bacteria count is 20,000,000,000 cfu/g in the fermenting agent, and Trichoderma viride total bacteria count is 500,000,000 cfu/g, is added
Dosage is 2.0kg/ ton dry materials.
5. preventing and treating root-knot nematode biological organic fertilizer according to claim 4, it is characterised in that the system of the carrier
Preparation Method includes：Chicken manure, mushroom slag, rape cake are mixed by proportioning, then add fermenting agent, is well mixed, regulation
Moisture is to 50%-60%, the high 1.0-1.5 rice of heap, stack retting 20-30 days；Cover film when fermenting initial, is treated in fermentation heap
Heart temperature removes film when reaching 50 DEG C, when fermentation heap central temperature is more than 65 DEG C with turning machine turning, 10 before fermentation
Its appropriate moisturizing so that fermentation heap moisture maintains more than 50%；So operate repeatedly, treat fermentation heap color become it is coffee-like or
Black, while fermentation heap temperature is down to less than 35 DEG C, terminates to ferment when water content is below 38%, the thing that will be fermented
Matter drying, broken, sieving, produce carrier.
6. the preventing and treating root-knot nematode biological organic fertilizer according to claim any one of 1-5, it is characterised in that described
The preparation method of composite bacteria agent, comprises the following steps：
I. the preparation of bacillus subtilis bacterium powder：Bacillus subtilis original strain is aseptically subjected to inclined-plane successively
After culture, shaking table culture, fermentation tank culture, obtained zymotic fluid is mixed with cornstarch, prepared by being concentrated and dried
Into Bacillus globigii spores powder；
II. the preparation of Paecilomyces lilacinus conidia powder：Paecilomyces lilacinus strain is aseptically subjected to inclined-plane training successively respectively
Support, after shaking table culture, fermentation tank culture, solid fermentation production spore, obtained solid fermentation product is concentrated and dried and is prepared into
Paecilomyces lilacinus conidia powder；
III. the preparation of thick wall spore Pu Keniya bacterium conidia powders：By thick wall spore Pu Keniya bacterium original strain aseptically according to
After carrying out inclined-plane culture, shaking table culture, fermentation tank culture, solid fermentation production spore, obtained solid fermentation product is concentrated
Drying is prepared into thick wall spore Pu Keniya bacterium conidia powders；
IV. the preparation of streptomycete bacterium powder：Streptomycete original strain is aseptically carried out to inclined-plane culture, shaking table training successively
Support, after fermentation tank culture, obtained zymotic fluid is mixed with cornstarch, streptomycete bacterium powder is prepared into by concentrate drying；
V. by bacillus subtilis bacterium powder obtained above, Paecilomyces lilacinus conidia powder, thick wall spore Pu Keniya bacterium conidia powder,
Streptomycete bacterium powder mixes in proportion, produces composite bacteria agent.
7. preventing and treating root-knot nematode biological organic fertilizer according to claim 6, it is characterised in that the composite bacteria agent
Preparation process I, II, III, IV specifically include：
I. the preparation of bacillus subtilis mixing conidia powder：
A. inclined-plane culture：Bacillus subtilis original strain is aseptically inoculated on slant medium,
36-48 hours are cultivated under the conditions of 35 ± 2 DEG C；
B. shaking table culture：The strain that step a is cultivated aseptically is inoculated in seed culture medium,
PH6.5-7.0, under the conditions of 35 DEG C, 140-160r/min shaking table culture 12-18 hours；
C. fermentation tank culture：The strain that step b is cultivated aseptically is inoculated in liquid fermentation medium,
PH7.5-8.0, tank pressure 0.5kg, 35 DEG C, ventilation 1:Under the conditions of 0.8-1.1, after cultivating 48-56 hours, viable count is big
In 1.0 × 109Cfu/mL, 80% thalline turn into gemma tank at present, obtain zymotic fluid；
D. by the zymotic fluid obtained in step c by etc. weight than mixing cornstarch, be prepared into withered grass bud by concentrate drying
Spore bacillus mixes conidia powder；
Wherein, the formula of the slant medium used in step a is as follows：Glucose 15g, fish peptone 5g, yeast extract
5g, agar 15g and water 1000mL；The seed culture based formulas used in step b is as follows：Glucose 10g, beef
Cream 5g, dusty yeast 5g, starch 10g, beancake powder 5g, KH2PO4 0.5g、MgSO40.2g and water 1000mL；
The liquid fermentation medium formula used in step c is as follows：Corn flour 26kg, beancake powder 16kg, ammonium sulfate 4kg,
Glucose 8kg, dusty yeast 2.5kg, peptone 1.7kg and defoamer 50mL, add water to 600L；
II. the preparation of Paecilomyces lilacinus conidia powder：
E. Paecilomyces lilacinus original strain is aseptically inoculated on slant medium, cultivated under the conditions of 28 DEG C
72 hours；
F. shaking table culture：The strain that step e is cultivated aseptically is inoculated in seed culture medium,
PH6.0-6.5, under the conditions of 26 DEG C, 160r/min shaking table cultures 24 hours；
G. fermentation tank culture：The step f strains cultivated aseptically are inoculated in liquid fermentation medium respectively,
In pH6.0-7.0, tank pressure 0.5kg, 26 DEG C, ventilation 1:0.6-0.8, after culture 72 hours, mycelium accounts for always
Volume 20% when terminate fermentation, carry out solid fermentation production spore；
H. solid fermentation production spore：Mycelium of the step g after fermentation tank culture is inoculated on solid fermentation culture medium,
168-180 hours are cultivated, stop fermentation when Paecilomyces lilacinus 90% produces spore；
I. the solid fermentation product obtained in step h is concentrated and dried and is prepared into Paecilomyces lilacinus conidia powder；
Wherein, the formula of the slant medium used in step e is as follows：Glucose 20g, murphy juice 200g, agar 20g
It is natural with water 1000mL, pH；The seed culture based formulas used in step f is as follows：White sugar 20g, peptone 5g,
Potassium dihydrogen phosphate 0.2g, magnesium sulfate 0.2g, sodium chloride 2g and water 1000mL；The liquid fermentation training used in step g
It is as follows to support based formulas：Starch 12kg, corn flour 3kg, beancake powder 1.2kg, white sugar 12kg, peptone 3kg, sulfuric acid
Magnesium 0.12kg, sodium chloride 12kg, defoamer 50mL, add water to 600L；Solid fermentation culture medium in step h is matched somebody with somebody
Side is as follows：Wheat bran, corn flour and beancake powder press 7:2:1 weight adds water in the solid material than mixing composition, produces solid
The weight ratio of fermentation medium, solid material and water is 1:0.6；
III. the preparation of thick wall spore Pu Keniya bacterium conidia powders：
J. aseptically, thick wall spore Pu Keniya bacterium strains are inoculated on slant medium, cultivated at 28 DEG C
5-7 days；
K. shaking table culture：The strain that step j is cultivated aseptically is inoculated in seed culture medium, in 28 DEG C of conditions
Under, 160r/min shaking table cultures 72 hours；
L. fermentation tank culture：Aseptically, in strain access liquid fermentation medium step k cultivated, first
Beginning pH7.0-8.0, tank pressure 0.5kg, 28 DEG C, ventilation 1:0.5-1,48-60 hours are cultivated, terminate fermentation, consolidate
Body fermentation production spore；
M. solid fermentation production spore：Mycelium of the step l after fermentation tank culture is inoculated on solid fermentation culture medium,
Culture 8-10 days, stop fermentation when thick wall spore Pu Keniya bacterium 90% produce spore；
The solid fermentation product obtained in m is concentrated and dried and is prepared into thick wall spore Pu Keniya bacterium conidia powders by n；
Wherein, the formula of the slant medium used in step j is as follows：Sucrose 20g, murphy juice 200g, agar 20g
It is natural with water 1000mL, pH；The seed culture based formulas used in step k is as follows：Sucrose 30g, sodium nitrate 5g,
Potassium dihydrogen phosphate 1g, magnesium sulfate 0.5g, potassium chloride 0.5g, ferrous sulfate 0.02g and water 1000mL；Make in step l
Liquid fermentation medium formula is as follows：Corn flour 12kg, soy meal 3kg, starch 1.2kg, sucrose 30g, nitric acid
Sodium 5g, potassium dihydrogen phosphate 1g, magnesium sulfate 0.5g, potassium chloride 0.5g, ferrous sulfate 0.02g defoamer 50mL, add
Water is to 600L；Solid fermentation culture medium prescription in step m is as follows：Corn flour, soy meal and wheat bran press 7:2:1 weight
Than the solid material of mixing composition, 2% sucrose, 1% sodium nitrate, 1% sodium chloride are re-added by material, adds water to mix thoroughly, produces
The weight ratio of solid fermentation culture medium, solid material and water is 1:0.5；
IV. the preparation of streptomycete bacterium powder：
O. aseptically, streptomyces species are inoculated on slant medium, cultivated 5-6 days at 28 DEG C；
P. shaking table culture：The strain that step o is cultivated aseptically is inoculated in seed culture medium, in 28 DEG C of conditions
Under, 220r/min shaking table cultures 24 hours；
Q. fermentation tank culture：Aseptically, in strain access liquid fermentation medium step p cultivated, first
Beginning pH7.5, tank pressure 0.5kg, 28 DEG C, ventilation 1:0.5-1, after cultivating 48 hours, put tank and collect zymotic fluid, add jade
Conidia powder is made after the concentrated drying of rice starch；
Wherein, the formula of the slant medium used in step o is as follows：Soluble starch 20g, KNO3 1g、NaCl0.5g、
K2HPO4 0.5g、MgSO4 0.5g、FeSO40.1g, agar powder 18g and distilled water 1000mL, pH7.2-7.4；Step
The culture medium prescription used in rapid p, q is as follows：Cornstarch 20g, bean powder 10g, NaCl0.5g, K2HPO4 0.5g、
(NH4)2SO4 5g、CaCO33g, defoamer 0.5g and distilled water 1000mL, pH7.5.
8. the preventing and treating root-knot nematode biological organic fertilizer according to claim any one of 1-7, it is characterised in that described
The deposit number of bacillus subtilis is ACCC 11025, the deposit number of Paecilomyces lilacinus is ACCC 30673, thick
The deposit number of wall spore Pu Keniya bacterium is ACCC 30601, the deposit number of streptomycete is ACCC 40460.
9. the preparation method of any one of the claim 1-8 preventing and treating root-knot nematode biological organic fertilizers, comprises the following steps：
(1) preparation of carrier；
(2) preparation of composite bacteria agent；
(3) preparation of root-knot nematode biological organic fertilizer is prevented and treated：The carrier is mixed in proportion with composite bacteria agent, i.e.,
Root-knot nematode biological organic fertilizer must be prevented and treated.
10. preventing and treating root-knot nematode biological organic fertilizer or claim 9 methods described system described in claim any one of 1-8
Application of the standby preventing and treating root-knot nematode biological organic fertilizer in crop-planting；
Preferably, the preventing and treating root-knot nematode biological organic fertilizer is applied as base manure, every mu of dosage 100-300kg；
Or used as auxiliary base manure, every mu of dosage 20-40kg.
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| CN112063536B (en) * | 2020-09-04 | 2022-07-05 | 青岛和协生物科技有限公司 | Pochonia chlamydosporia for preventing and treating root knot nematode disease, composite powder and application |
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| CN112314639A (en) * | 2020-11-03 | 2021-02-05 | 广东省农业科学院茶叶研究所 | Biological pesticide fertilizer for preventing and treating tea seedling root knot nematode disease and preparation method thereof |
| CN112458017A (en) * | 2020-12-01 | 2021-03-09 | 鹤山市新的生物制品有限公司 | Bacillus subtilis XDK06 and application thereof |
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