CN108165272A - A kind of microorganism agricultural land soil renovation agent and its restorative procedure - Google Patents

A kind of microorganism agricultural land soil renovation agent and its restorative procedure Download PDF

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Publication number
CN108165272A
CN108165272A CN201711440745.XA CN201711440745A CN108165272A CN 108165272 A CN108165272 A CN 108165272A CN 201711440745 A CN201711440745 A CN 201711440745A CN 108165272 A CN108165272 A CN 108165272A
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soil
renovation agent
microorganism
agricultural land
agent
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CN201711440745.XA
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Inventor
谢正宇
李文峰
储文涛
朱长虹
杨静
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Beijing Shanghua Ecological Environmental Protection & Technology Stock Co
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Beijing Shanghua Ecological Environmental Protection & Technology Stock Co
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Priority to CN201711440745.XA priority Critical patent/CN108165272A/en
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    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K17/00Soil-conditioning materials or soil-stabilising materials
    • C09K17/14Soil-conditioning materials or soil-stabilising materials containing organic compounds only
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01BSOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
    • A01B79/00Methods for working soil
    • A01B79/02Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K2101/00Agricultural use

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Soil Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Mechanical Engineering (AREA)
  • Environmental Sciences (AREA)
  • General Life Sciences & Earth Sciences (AREA)
  • Materials Engineering (AREA)
  • Organic Chemistry (AREA)
  • Processing Of Solid Wastes (AREA)
  • Soil Conditioners And Soil-Stabilizing Materials (AREA)

Abstract

The invention discloses a kind of microorganism agricultural land soil renovation agent and its restorative procedure, renovation agent includes culture medium and microorganism;Culture medium includes brown sugar or white granulated sugar, sterile water and nutritive salt.Microorganism is mixed by one or more of actinomyces, lactic acid bacteria, saccharomycete, phosphate solubilizing bacteria, potassium solubilizing bacteria, nitrogen-fixing bacteria, bacillus subtilis, bacillus megaterium, bacillusmusilaginosiengineering and bacillus thuringiensis.The method that soil remediation is carried out using mentioned microorganism agricultural land soil renovation agent, the native condition after acquisition including pedotheque and detection, the ingredient allotment of soil-repairing agent, the using and use of soil-repairing agent are kept.Active ingredient in soil-repairing agent can be in curing soil activated state heavy metal, its toxic action to crops is reduced, the growth for crops provides good environment, realizes the plantation of the crops in the greenhouse of heavy metal pollution region, pass through the comparison of field trial, significant effect.

Description

A kind of microorganism agricultural land soil renovation agent and its restorative procedure
Technical field
The present invention relates to technical field of soil remediation, and in particular to a kind of microorganism agricultural land soil renovation agent and its reparation side Method.
Background technology
The mankind multiply and live on soil for generations, are provided by being cultivated on soil to obtain endlessly existence Source.At present, chemical fertilizer and pesticide are used in large quantities in order to increase production and improve productivity in farmland, cause heavy metal in soil content exceeded, Soil hardening is serious, and eventually leading to crop production reduction can not even grow.Soil receives the pollution of heavy metal, the agriculture of cultivation Field soil environment is gradually deteriorated.Heavy metal can be finally enriched to by food chain in mankind's body, to the health band of eater Carry out security risk.The pollution of cultivated soil and the underproduction of grain all hinder the development of agriculture health, it is therefore desirable to effective and feasible Soil remediation method.
The administering method of farmland soil heavy metals pollution spatially has in-situ immobilization and showering to divide, by processing method There are engineering measure, chemical measure, microorganism measure to divide.Soil remediation method cuts both ways:Showering compares more thoroughly but grasps Make difficulty it is big, it is with high costs and be not suitable for large area farmland reparation.Engineering measure is digged including heavy metal pollution of soil, soil replacement method, deep ploughing Method, curing, stabilization method, Electroremediation method etc., engineering measure have the advantages that stablize, are quick, but there are quantities Greatly, the shortcomings of investment cost height, secondary pollution hidden danger, it is not suitable for the improvement of pollution in wide area soil.Chemical harnessing measure includes Leaching method, using modifying agent the methods of, can reduce the toxicity and biological effectiveness of heavy metal in soil in a short time, but because Artificial applies chemical agent into soil, and chemical agent excessively launches the secondary pollution for easily causing soil.
Invention content
In view of the above-mentioned problems, the present invention provides a kind of microorganism agricultural land soil renovation agent and its restorative procedure, to solve agriculture Field heavy metal pollution of soil, crop growth are bad, soil remediation is of high cost, quantities is big, time-consuming, are excessively thrown there are medicament Put the technical problems such as soil secondary pollution.
One aspect of the invention provides a kind of microorganism agricultural land soil renovation agent, and the renovation agent includes culture medium and micro- life Object;The culture medium includes brown sugar or white granulated sugar, sterile water and nutritive salt.
Further, the microorganism be actinomyces, lactic acid bacteria, saccharomycete, phosphate solubilizing bacteria, potassium solubilizing bacteria, nitrogen-fixing bacteria, withered grass gemma One or more of bacillus, bacillus megaterium, bacillusmusilaginosiengineering and bacillus thuringiensis;Each microbial strains Mass percent is:Actinomyces 1.1%~1.3%, lactic acid bacteria 0.8%~1.0%, saccharomycete 0.9%~1.1%, phosphate solubilizing bacteria 1.1%~1.2, potassium solubilizing bacteria 1.1%~1.2, nitrogen-fixing bacteria 0.8%~1.6%, bacillus subtilis 0.7%~1.3% is huge Bacillus 1%~1.5%, bacillusmusilaginosiengineering 0.5%~1% and bacillus thuringiensis 0.7%~1.2%.
Further, the mass percent of brown sugar or white granulated sugar is 2%~3% in the culture medium, the quality of nutritive salt Percentage is 0.1%~0.35%.
Further, viable count is not less than 2.5 hundred million/ml in the renovation agent.
Another aspect of the present invention provides the method that soil remediation is carried out using mentioned microorganism agricultural land soil renovation agent, described After the acquisition of method including pedotheque and detection, the ingredient allotment of soil-repairing agent, the using and use of soil-repairing agent Native condition keep, specifically carry out in the steps below:
Step 1 acquires pedotheque and detects heavy metal component and activated state content;
Step 2 targetedly matches soil-repairing agent according to detection data;
Step 3 measures soil moisture content;
Step 4 in pending upper soll layer organic fertilizer, will be sprayed at pending soil after soil remediation dilution agent Earth surface layer, plowed soils after completion;
Step 5, earthing heat preservation, canopy film lowering, into soil remediation process.
Further, the step 3 measures soil moisture content, and by water content control 30%~50%.
Further, the fowl and animal excrement be chicken manure, one or more of sheep manure and cow dung.
Further, ploughed depth 20cm~40cm.
Further, temperature of shed is 20 DEG C~40 DEG C in step 5.
Further, the renovation agent is covered on the soil repaired, and the dosage of microorganism renovation agent is per acre 10~30L.
Further, the step 4 soil-repairing agent makes to be diluted with water, dilution ratio 1: 80~100.
In some preferred embodiments, microorganism agricultural land soil renovation agent is by 2% brown sugar, 0.25% nutrition Salt, 1.2% actinomyces, 1.1% potassium solubilizing bacteria, 0.9% lactic acid bacteria, 1% saccharomycete, 1.1% phosphate solubilizing bacteria and nothing Bacterium water forms.
Beneficial effects of the present invention are embodied in:
(1) a kind of microorganism agricultural land soil renovation agent of the present invention and its restorative procedure, the restorative procedure are repaiied based on microorganism Recovering technology specifically being used and being used by the ingredient allotment of the acquisition of pedotheque and detection, soil-repairing agent, soil-repairing agent The sport technique segments such as native condition holding later are formed.Active ingredient in soil-repairing agent can be in curing soil an activated state huge sum of money Belong to, reduce its toxic action to crops, the growth for crops provides good environment, realizes in heavy metal pollution area The plantation of crops in the greenhouse of domain, passes through the comparison of field trial, significant effect.
(2) microorganism agricultural land soil renovation agent provided by the invention, proportioning is scientific and reasonable and can targetedly optimize The microorganism species of soil improve soil physico-chemical property, enhance the microbial activity of soil.Edaphic condition after reparation is good, Quality is loose, and organic substance utilization rate increases.User can make contaminated soil gradually by constantly adjusting renovation agent component Restore purification function and pooling feature.Arable soil after reparation can promote plant growth, inhibit soil-borne disease.
(3) a kind of microorganism agricultural land soil renovation agent of the present invention and its restorative procedure, have at low cost, quick, easy behaviour The advantages that work, effect stability, non-secondary pollution, the present invention have the function of to cast a brick to attract jade, and will make microorganism in situ soil remediation Technology gradually becomes the main R&D direction of agricultural land soil reparation.
Microorganism agricultural land soil renovation agent and its restorative procedure provided by the invention can be widely applied to agricultural, forestry and The fields such as soil improvement, to solve the problems, such as that heavy metal-polluted soil harms the crops growth.
Other features and advantages of the present invention will be illustrated in the following description, and partly becomes from specification It is clear that understood by implementing the present invention.The main object of the present invention and further advantage can be by specification, power Specifically noted scheme is realized and is obtained in sharp claim.
Specific embodiment
As used herein, term "comprising", " comprising ", " containing ", " having " meaning be non-limiting, you can add in Other steps of result and other ingredients are not influenced.Terms above cover term " by ... form " and " substantially by ... group Into ".Unless otherwise specified, material, equipment, reagent are commercially available.
The present invention provides a kind of microorganism agricultural land soil renovation agent, and the renovation agent includes culture medium and microorganism;It is described Culture medium includes brown sugar or white granulated sugar, sterile water and nutritive salt.
Wherein, the microorganism is by actinomyces, lactic acid bacteria, saccharomycete, phosphate solubilizing bacteria, potassium solubilizing bacteria, nitrogen-fixing bacteria, bacillus subtilis One or more of bacterium, bacillus megaterium, bacillusmusilaginosiengineering and bacillus thuringiensis mix;Each microorganism The mass percent of bacterial strain is:Actinomyces 1.1%~1.3%, lactic acid bacteria 0.8%~1.0%, saccharomycete 0.9%~1.1%, Phosphate solubilizing bacteria 1.1%~1.2, potassium solubilizing bacteria 1.1%~1.2, nitrogen-fixing bacteria 0.8%~1.6%, bacillus subtilis 0.7%~ 1.3%th, bacillus megaterium 1%~1.5%, bacillusmusilaginosiengineering 0.5%~1% and bacillus thuringiensis 0.7%~ 1.2%.
The mass percent of brown sugar or white granulated sugar is 2%~3% in the culture medium, and the mass percent of nutritive salt is 0.1%~0.35%, viable count is not less than 2.5 hundred million/ml in the microbial inoculum.
The present invention also provides a kind of method that soil remediation is carried out using mentioned microorganism agricultural land soil renovation agent, the sides After the acquisition of method including pedotheque and detection, the ingredient allotment of soil-repairing agent, the using and use of soil-repairing agent Native condition is kept, and is specifically carried out in the steps below:
Step 1 acquires pedotheque and detects heavy metal component and activated state content (referring to table 3);Specifically, it utilizes Cutting ring formula original-state soil sampler layouts method acquisition pedotheque (using snake if greenhouse is more long and narrow using checkerboard type Shape is layouted method), 5/mu of pedotheque quantity (increases and decreases) in proportion according to real area, and acquisition utilizes GB15618- after completing 1995 methods detection content of beary metal (testing agency can be transferred to detect);
Step 2, the detection data provided according to table 3 targetedly match soil-repairing agent (Examples 1 to 4, proportioning Refer to table 1);
Step 3 measures soil moisture content, and by water content control 30%~50%.If moisture deficit needs to water Moisturizing.
Step 4 is normal by crops by the organic fertilizer of fowl and animal excrement (fowl and animal excrement can be chicken manure, sheep manure, cow dung etc.) Growth aequum is uniformly applied in pending soil surface, pending soil table will be sprayed at after soil remediation dilution agent Layer completes later plowed soils immediately, ploughed depth 20cm~40cm;Soil-repairing agent makes to be diluted with water, and ratio 1: 80~ 100。
Step 5, after Soil tillage is completed, by temperature control at 20 DEG C~40 DEG C, canopy film lowering is kept for earthing heat preservation Anaerobic environment in greenhouse, soil enter repair process.If the processing time is summer, timing is needed to open canopy mouth aeration-cooling.Institute It states Step 3: step 4 and step 5 needs were completed in 24 hours.After soil-repairing agent, 5~8 days are microorganism soil During which the reparation phase of earth renovation agent cannot carry out farming operations and must not use any fertilizer and pesticide.Soil is repaired according to current The data that earth obtains, renovation agent are covered on the soil repaired, and dosage is 10~30L per acre.Renovation agent used in amounts It to be set according to the amount of organic fertilizer.Each ton of organic fertilizer averagely matches 10L bacterium solutions.
It should be noted that microorganism agricultural land soil renovation agent provided by the invention is only capable of and using fowl and animal excrement class as raw material Organic fertilizer be used cooperatively.
Examples 1 to 4 is the experiment that inventor carries out in Qingzhou City village of Shandong Province, and local farmers due to largely making for a long time With fertilizer and pesticide so that heavy metal pollution of soil problem is very prominent, has seriously affected the income of local farmers, has limited locality The development of agricultural.The method that we use check experiment in locality compared traditional mode with using at two kinds of soil-repairing agent By the time (5~6 months or so) of sharp one Growing season of green pepper under reason mode, the variation before and after heavy metal content in soil.
It is as shown in table 1 below by after extracting pedotheque and measuring its content of beary metal, preparing renovation agent:
The component proportion of 1 Examples 1 to 4 of table
When carrying out soil remediation construction, the soil remediation response parameter of Examples 1 to 4 is as shown in table 2 below:
2 soil remediation response parameter of table
Table 3:The forward and backward each contents of heavy metal elements variation of control group processing
Brief summary:
As can be seen from Table 3, traditional planting patterns is due to the use of chemical fertilizer, and heavy metal pollution of soil is serious after harvesting.Make Soil remediation is carried out with microorganism renovation agent provided by the invention, and the different degrees of reduction of the heavy metal in soil, explanation is matched Close a kind of microorganism agricultural land soil renovation agent of present invention offer has apparent inhibiting effect to activated state content of beary metal in soil.
Based on above-described embodiment 1~4, a kind of microorganism agricultural land soil renovation agent provided by the invention, contaminated soil sampling Science, proportioning is reasonable, and method is effective.Active ingredient in soil-repairing agent can targetedly in curing soil activated state Heavy metal, reduces its toxic action to crops, and the growth for crops provides good environment, has a extensive future.
The foregoing is merely the preferable specific embodiment of the present invention, but protection scope of the present invention is not limited thereto, The change or replacement that any one skilled in the art is expected in the technical scope disclosed by the present invention should all be contained Lid is within protection scope of the present invention.

Claims (10)

1. a kind of microorganism agricultural land soil renovation agent, which is characterized in that the renovation agent includes culture medium and microorganism;The training It supports base and includes brown sugar or white granulated sugar, sterile water and nutritive salt.
2. microorganism agricultural land soil renovation agent as described in claim 1, which is characterized in that the microorganism is actinomyces, newborn Sour bacterium, saccharomycete, phosphate solubilizing bacteria, potassium solubilizing bacteria, nitrogen-fixing bacteria, bacillus subtilis, bacillus megaterium, bacillusmusilaginosiengineering and Soviet Union One or more of cloud gold bacillus;The mass percent of each microbial strains is:Actinomyces 1.1%~1.3%, lactic acid Bacterium 0.8%~1.0%, saccharomycete 0.9%~1.1%, phosphate solubilizing bacteria 1.1%~1.2, potassium solubilizing bacteria 1.1%~1.2, nitrogen-fixing bacteria 0.8%~1.6%, bacillus subtilis 0.7%~1.3%, bacillus megaterium 1%~1.5%, bacillusmusilaginosiengineering 0.5%~1% and bacillus thuringiensis 0.7%~1.2%.
3. microorganism agricultural land soil renovation agent as described in claim 1, which is characterized in that brown sugar or white in the culture medium The mass percent of granulated sugar is 2%~3%, and the mass percent of nutritive salt is 0.1%~0.35%.
4. microorganism agricultural land soil renovation agent as described in claim 1, which is characterized in that viable count is not low in the renovation agent In 2.5 hundred million/ml.
5. the method for soil remediation is carried out using microorganism agricultural land soil renovation agent as described in claim 1, which is characterized in that Ingredient allotment, soil-repairing agent the method includes acquisition and detection, the soil-repairing agent of pedotheque using and using Native condition later is kept, and is specifically carried out in the steps below:
Step 1 acquires pedotheque and detects heavy metal component and activated state content;
Step 2 targetedly matches soil-repairing agent according to detection data;
Step 3 measures soil moisture content;
Step 4 in pending upper soll layer organic fertilizer, will be sprayed at pending soil table after soil remediation dilution agent Layer, plowed soils after completion;
Step 5, earthing heat preservation, canopy film lowering, into soil remediation process.
6. the method that microorganism agricultural land soil renovation agent as claimed in claim 5 carries out soil remediation, which is characterized in that described Step 3 measures soil moisture content, and by water content control 30%~50%.
7. the method that microorganism agricultural land soil renovation agent as claimed in claim 5 carries out soil remediation, which is characterized in that described Fowl and animal excrement is chicken manure, one or more of sheep manure and cow dung.
8. the method that microorganism agricultural land soil renovation agent as claimed in claim 5 carries out soil remediation, which is characterized in that turn over Depth 20cm~40cm.
9. the method that microorganism agricultural land soil renovation agent as claimed in claim 5 carries out soil remediation, which is characterized in that step Temperature of shed is 20 DEG C~40 DEG C in five.
10. the method that microorganism agricultural land soil renovation agent as claimed in claim 5 carries out soil remediation, which is characterized in that institute It states renovation agent to cover on the soil repaired, the dosage of microorganism renovation agent is 10~30L per acre.
CN201711440745.XA 2017-12-26 2017-12-26 A kind of microorganism agricultural land soil renovation agent and its restorative procedure Pending CN108165272A (en)

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Cited By (6)

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Publication number Priority date Publication date Assignee Title
CN110180888A (en) * 2019-04-15 2019-08-30 沈阳大学 A kind of method that blood meal enhancement microbiological repairs DDTs polluted farmland soil
CN110355196A (en) * 2019-07-12 2019-10-22 重庆丰衣谷农业科技有限公司 A kind of microbe soil restoration agent and its rehabilitating soil method
CN111777469A (en) * 2020-07-17 2020-10-16 盘锦鑫叶农业科技有限公司 Greenhouse soil remediation agent based on reed leaves and spikes and soil remediation method thereof
CN111849824A (en) * 2020-07-29 2020-10-30 郑州浩创农业科技有限公司 Quality-improving microbial agent and preparation method thereof
CN112898989A (en) * 2021-01-22 2021-06-04 郭芳利 Remediation agent for farmland soil pollution and preparation method thereof
CN114600584A (en) * 2022-02-28 2022-06-10 河南城建学院 Method for rapidly repairing hardening obstacle of reclamation cultivated land of damaged land in mining area

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110180888A (en) * 2019-04-15 2019-08-30 沈阳大学 A kind of method that blood meal enhancement microbiological repairs DDTs polluted farmland soil
CN110355196A (en) * 2019-07-12 2019-10-22 重庆丰衣谷农业科技有限公司 A kind of microbe soil restoration agent and its rehabilitating soil method
CN111777469A (en) * 2020-07-17 2020-10-16 盘锦鑫叶农业科技有限公司 Greenhouse soil remediation agent based on reed leaves and spikes and soil remediation method thereof
CN111849824A (en) * 2020-07-29 2020-10-30 郑州浩创农业科技有限公司 Quality-improving microbial agent and preparation method thereof
CN112898989A (en) * 2021-01-22 2021-06-04 郭芳利 Remediation agent for farmland soil pollution and preparation method thereof
CN114600584A (en) * 2022-02-28 2022-06-10 河南城建学院 Method for rapidly repairing hardening obstacle of reclamation cultivated land of damaged land in mining area

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Application publication date: 20180615