CN106906162A - Agricultural microorganism bacterial manure and preparation method and application - Google Patents
Agricultural microorganism bacterial manure and preparation method and application Download PDFInfo
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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Abstract
The present invention relates to a kind of agricultural microorganism bacterial manure and preparation method and application, agricultural microorganism bacterial manure includes culture medium and thalline, and thalline includes：Bacillus subtilis, bacillus megaterium, bacillusmusilaginosiengineering, bacillus licheniformis, bacillus thuringiensis, bacillus laterosporus, colloid bacillus cereus, Streptomyces jingyangensis, azotobacter vinelandii, bacillus coagulans, aspergillus oryzae and Paecilomyces lilacinus.The agricultural microorganism bacterial manure that the present invention is provided, can be supplemented the nutrients, to soil with effectively preventing part pest and disease damage with improved soil effectively to solve the long-standing problem of soil hardening；And phosphorus and potassium for existing with chelate in soil etc. can be made effectively to discharge with phosphorus decomposing, potassium decomposing.
Description
Technical field
The present invention relates to microbial technology field, and in particular to a kind of agricultural microorganism bacterial manure and preparation method thereof with should
With.
Background technology
With the development of society, various environmental problems are increasingly severe, the dirt such as various industrial waste gases, waste water, chemical fertilizer, agricultural chemicals
Dye thing all enters in its final place body-soil, causes soil environment quality increasingly to decline, especially heavy metal pollution of soil.Weight
Metal ion is micro- necessary to be plant metabolism, but has suitable toxicity if they are excessive, causes already complete
Social and the great attention of the public.Because heavy-metal contaminated soil structure is poor, nutrient dificiency, heavy metal is stronger with toxicity
Form exist, so as to influence the growth of plant.Therefore, pollution source, form, biological effectiveness and controlled accordingly in soil
The research of reason means is extremely necessary.To administer the various pollutions of soil, various modifying agents are generally added to improve the thing of soil
Physicochemical property, promotes plant growth, strengthens the effect of phytoremediation.There is many modifying agents, but big portion in the prior art
Point there is improved effect not ideal enough, the improvement cycle is long, cures the symptoms, not the disease, it is small to administer area, should not widely use and wait deficiency, from
And limit promoting the use of for modifying agent.Accordingly, it would be desirable to develop new soil improvement material to avoid existing in the prior art
Shortcoming.
The content of the invention
The invention provides a kind of agricultural microorganism bacterial manure, including culture medium and thalline, the thalline includes：Withered grass gemma
Bacillus (Bacillus subtilis), bacillus megaterium (Bacillus megaterium), bacillusmusilaginosiengineering
(Bacillus mucilaginosus), bacillus licheniformis (Bacillus licheniformis), bacillus thuringiensis
(Bacillus thuringiensis) and bacillus laterosporus (Bacillus laterosporus), colloid bacillus cereus
(Bacillus mucilaginosus), Streptomyces jingyangensis (Streptomyces jingyangensis), azotobacter vinelandii
(Azotobactervinelandii), bacillus coagulans (Bacillus coagulans), aspergillus oryzae (Aspergillus
) and Paecilomyces lilacinus (Paecilomyces lilacinus) oryzae.
In agricultural microorganism bacterial manure, the viable count of bacillus subtilis is (0.5~5) × 108CFU/g, huge gemma
The viable count of bacillus is (0.5~5) × 108CFU/g, the viable count of bacillusmusilaginosiengineering is (0.5~5) × 108CFU/g, ground
The viable count of clothing bacillus is (1~3) × 108CFU/g, the viable count of bacillus thuringiensis is (1~5) × 108CFU/g,
The viable count of bacillus laterosporus is (1~3) × 108CFU/g, the viable count of colloid bacillus cereus is (1~3) × 108CFU/g,
The viable count of Streptomyces jingyangensis is (1~3) × 108CFU/g, the viable count of azotobacter vinelandii is (1~3) × 108CFU/g, condenses
The viable count of bacillus is (1~3) × 108CFU/g, the viable count of aspergillus oryzae is (1~5) × 108CFU/g, Paecilomyces lilacinus
Viable count be (1~3) × 108CFU/g。
According to the present invention, the species of culture medium can change in very large range, can be used in cultivating withered for various
Careless bacillus, bacillus megaterium, bacillusmusilaginosiengineering, bacillus licheniformis, bacillus thuringiensis, side spore gemma bar
The culture of bacterium, colloid bacillus cereus, Streptomyces jingyangensis, azotobacter vinelandii, bacillus coagulans, aspergillus oryzae and Paecilomyces lilacinus
Base, for example, can be one or more in following culture medium：(1) for cultivating bacillus licheniformis and bacillus subtilis
Nutrient culture medium, such as on the basis of the gross weight of the culture medium, the percentage composition of peptone can be in the culture medium
0.25%~1%, the percentage composition of NaCl can be 0.25%~1%, percentage composition of beef extract can for 0.2%~
0.5%, balance of sterilized water, the pH value of the culture medium is 7.0；(2) for cultivating the YPG culture mediums of bacillus coagulans, with
On the basis of the gross weight of the culture medium, the percentage composition of peptone can be 0.25%~1%, the hundred of glucose in the culture medium
Divide content be able to be 0.2%~0.8%, balance of sterilized water, the training for the percentage composition of 0.25%~1%, yeast extract
The pH value for supporting base is 7.2；(3) a culture medium is synthesized for cultivating the Gao Shi of Streptomyces jingyangensis, with the gross weight of the culture medium
On the basis of, the percentage composition of soluble starch can be 1%~5%, KNO in the culture medium3Percentage composition can be 0.1%
~0.5%, K2HPO4Percentage composition can be 0.25%~0.8%, NaCl percentage composition can for 0.01%~
0.05%, balance of sterilized water, the pH value of the culture medium is 7.2；(4) for cultivate bacillus megaterium, colloid bacillus cereus,
The culture medium of bacillusmusilaginosiengineering, azotobacter vinelandii, includes per 100g culture mediums：2~6g of sucrose, 5~15g of mannitol,
1~2g of ammonium sulfate, 1~2g of peptone, 2~4g of beef extract, 1~3g of sodium chloride, potassium dihydrogen phosphate 0.15g, ammonium molybdate 0.6g, carbon
Sour calcium 0.02g, frerrous chloride 0.02g, magnesium sulfate 0.02g, balance of water, regulation pH value is 7.3；(5) for cultivating Su Yunjin
The culture medium of bacillus：In the culture medium of 100g, starch 3.0%~4.0%, beancake powder 1.8%~2.4%, cottonseed cake
Powder 1.2%~1.8%, peptone 0.5%~0.9%, dusty yeast 0.4%, corn pulp 2.0%, epsom salt 0.04%, phosphorus
The 1/400 of acid dihydride potassium 0.2%, dipotassium hydrogen phosphate 0.2%, calcium carbonate 0.1% and AMS/content of starch, it is balance of
Water, the pH value of the culture medium is 7.2；(6) for cultivating the culture medium of bacillus laterosporus：In the culture medium of 100g, beef
Cream 0.5g, peptone 1g, sodium chloride 0.5g, balance of water, pH value is 7.3；(7) for cultivating the culture medium of aspergillus oryzae：
In the culture medium of 100g, glucose 0.5%~4%, soya-bean milk 30%~65%, corn pulp 0.2%~3.5%, magnesium sulfate
0.05%~0.3%, dipotassium hydrogen phosphate 0.2%, balance of water, the pH value of the culture medium is 7.3；(8) for cultivating pale purple plan
The culture medium of mould：In the culture medium of 1000g, NaNO3 3g、K2HPO4 1g、KCl 0.5g、MgSO4·7H2O 0.5g、
FeSO40.01g, sucrose 30g, balance of water, the pH value of the culture medium is 7.3.
In the present invention, bacillus subtilis (Bacillus subtilis), bacillus megaterium (Bacillus
Megaterium), bacillusmusilaginosiengineering (Bacillus mucilaginosus), bacillus licheniformis (Bacillus
Licheniformis), bacillus thuringiensis (Bacillus thuringiensis), bacillus laterosporus (Bacillus
Laterosporus), colloid bacillus cereus (Bacillus mucilaginosus), Streptomyces jingyangensis (Streptomyces
Jingyangensis), azotobacter vinelandii (Azotobactervinelandii), bacillus coagulans (Bacillus
Coagulans), the bacterium of aspergillus oryzae (Aspergillus oryzae) and Paecilomyces lilacinus (Paecilomyces lilacinus)
Planting to be commercially available, and bought such as by domestic and international Spawn incubation company, also can be such as domestic by biomaterial center
CGMCC and CCTCC be commercially available.In the present invention, Bacillus subtilis bacillus (Bacillus subtilis)
Buy in China General Microbiological culture presevation administrative center, preserving number CGMCC NO:1.3376, bacillus megaterium
(Bacillus megaterium) is bought in China General Microbiological culture presevation administrative center, preserving number CGMCC NO:
1.8569, bacillusmusilaginosiengineering (Bacillus mucilaginosus) is bought in Chinese agriculture Microbiological Culture Collection
The heart, preserving number ACCC NO：10168, bacillus licheniformis (Bacillus licheniformis) is bought in Chinese common micro- life
Thing culture presevation administrative center, preserving number CGMCC NO:1.10314, bacillus thuringiensis (Bacillus
Thuringiensis) buy in China General Microbiological culture presevation administrative center, preserving number CGMCC NO:1.1753, side spore
Bacillus (Bacillus laterosporus) is bought in Chinese agriculture Culture Collection, preserving number ACCC
NO：11079, colloid bacillus cereus (Bacillus mucilaginosus) are bought to be received in Beijing North and create the connection limited public affairs of biotechnology
Department, model BNCC183950, Streptomyces jingyangensis (Streptomyces jingyangensis) are bought in the micro- life of Chinese agriculture
Thing DSMZ, preserving number ACCC NO：40126, azotobacter vinelandii (Azotobactervinelandii) is bought in north
Jing Bei Na Chuanlian Bioisystech Co., Ltd, model BNCC337393, bacillus coagulans (Bacillus coagulans)
Buy in China General Microbiological culture presevation administrative center, preserving number CGMCC NO:1.3220, aspergillus oryzae (Aspergillus
Oryzae) buy in China General Microbiological culture presevation administrative center, preserving number CGMCC NO:3.4383, Paecilomyces lilacinus
(Paecilomyces lilacinus) is bought in China General Microbiological culture presevation administrative center, preserving number CGMCC NO:
3.5374.China General Microbiological culture presevation administrative center address：Section of Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 China
Institute of microbiology of institute；Chinese agriculture Culture Collection address：No.12 ,zhongguancun south street,Haidian District, Beijing；
Beijing North Na Chuanlian Bioisystech Co., Ltd address：Chaoyang District, Beijing City Shuan Hui gardens No. 5 building of first.
Present invention also offers the preparation method of agricultural microorganism bacterial manure, comprise the following steps：By bacillus subtilis, huge
Bacterium anthracoides, bacillusmusilaginosiengineering, bacillus licheniformis, bacillus thuringiensis, bacillus laterosporus, colloid gemma bar
Bacterium, Streptomyces jingyangensis, azotobacter vinelandii, bacillus coagulans, aspergillus oryzae and Paecilomyces lilacinus are trained in being inoculated in culture medium
Support, the viable count for making bacillus subtilis is (0.5~5) × 108CFU/g, the viable count of bacillus megaterium is (0.5~5)
×108CFU/g, the viable count of bacillusmusilaginosiengineering is (0.5~5) × 108CFU/g, the viable count of bacillus licheniformis is (1
~3) × 108CFU/g, the viable count of bacillus thuringiensis is (1~5) × 108CFU/g, the viable count of bacillus laterosporus is
(1~3) × 108CFU/g, the viable count of colloid bacillus cereus is (1~3) × 108CFU/g, the viable count of Streptomyces jingyangensis is (1
~3) × 108CFU/g, the viable count of azotobacter vinelandii is (1~3) × 108CFU/g, the viable count of bacillus coagulans for (1~
3)×108CFU/g, the viable count of aspergillus oryzae is (1~5) × 108CFU/g, the viable count of Paecilomyces lilacinus for (1~3) ×
108CFU/g。
Present invention also offers the preparation method of another agricultural microorganism bacterial manure, comprise the following steps：Each independent
Cultivating system in respectively culture obtain bacillus subtilis, bacillus megaterium, bacillusmusilaginosiengineering, lichens gemma bar
Bacterium, bacillus thuringiensis, bacillus laterosporus, colloid bacillus cereus, Streptomyces jingyangensis, azotobacter vinelandii, condensation gemma bar
Bacterium, aspergillus oryzae and Paecilomyces lilacinus, are then proportionally mixed, so as to get agricultural microorganism bacterial manure in：Withered grass gemma
The viable count of bacillus is (0.5~5) × 108CFU/g, the viable count of bacillus megaterium is (0.5~5) × 108CFU/g, jelly
The viable count of sample bacillus is (0.5~5) × 108CFU/g, the viable count of bacillus licheniformis is (1~3) × 108CFU/g,
The viable count of bacillus thuringiensis is (1~5) × 108CFU/g, the viable count of bacillus laterosporus is (1~3) × 108CFU/
G, the viable count of colloid bacillus cereus is (1~3) × 108CFU/g, the viable count of Streptomyces jingyangensis is (1~3) × 108CFU/g,
The viable count of azotobacter vinelandii is (1~3) × 108CFU/g, the viable count of bacillus coagulans is (1~3) × 108CFU/g, rice
The viable count of aspergillus is (1~5) × 108CFU/g, the viable count of Paecilomyces lilacinus is (1~3) × 108CFU/g。
It should be noted that in the present invention, bacillus subtilis, bacillus megaterium, bacillusmusilaginosiengineering, lichens bud
Spore bacillus, bacillus thuringiensis, bacillus laterosporus, colloid bacillus cereus, Streptomyces jingyangensis, azotobacter vinelandii, condensation bud
The independent cultural method of spore bacillus, aspergillus oryzae and Paecilomyces lilacinus is known to those skilled in the art, that is, corresponding bacterium
The conventional cultural method planted.The condition of described mixing has no particular limits, as long as the agricultural microorganism for enabling to
Bacterial manure meets following condition：The viable count of bacillus subtilis is (0.5~5) × 108CFU/g, bacillus megaterium
Viable count is (0.5~5) × 108CFU/g, the viable count of bacillusmusilaginosiengineering is (0.5~5) × 108CFU/g, lichens gemma
The viable count of bacillus is (1~3) × 108CFU/g, the viable count of bacillus thuringiensis is (1~5) × 108CFU/g, side spore bud
The viable count of spore bacillus is (1~3) × 108CFU/g, the viable count of colloid bacillus cereus is (1~3) × 108CFU/g, Jingyang chain
The viable count of mould is (1~3) × 108CFU/g, the viable count of azotobacter vinelandii is (1~3) × 108CFU/g, condenses gemma bar
The viable count of bacterium is (1~3) × 108CFU/g, the viable count of aspergillus oryzae is (1~5) × 108CFU/g, the viable bacteria of Paecilomyces lilacinus
Number is (1~3) × 108CFU/g。
Present invention also offers a kind of method of soil enrichment, comprise the following steps：Agricultural micro- life that the present invention is provided
Thing bacterial manure is inoculated in soil, turns uniform after fermentation, obtains getting fat soil.It should be noted that micro- life that the present invention is provided
Thing bacterial manure, it is adaptable to various soil, improved effect for salt-soda soil it is further preferred that.
Wherein, the inoculum concentration of agricultural microorganism bacterial manure can change in very large range, agricultural microorganism bacterial manure and described
The mass ratio of soil is preferably (0.05~0.2):100.The condition of fermentation can change in very large range, and the temperature of fermentation is excellent
Elect 20~30 DEG C as, fermentation time is preferably 3~5 days.
The present invention also protection agricultural microorganism bacterial manure is in the soil used by getting fat planting vegetable, fruit tree, paddy rice, corn
Using vegetables include cabbage heart, and cabbage heart includes four or nine cabbage hearts.
Agricultural microorganism bacterial manure that the present invention is provided and preparation method and application, with following beneficial effect：
(1) the agricultural microorganism bacterial manure that the present invention is provided fundamentally solves conventional fertilizers and only promotes plant growth, only
To soil absorption nutrition, and cannot for and soil nutrient shortcoming；
(2) the agricultural microorganism bacterial manure that the present invention is provided can improve any soil, can be with effectively preventing part disease
Insect pest, can supplement the nutrients to soil, effectively to solve the long-standing problem of soil hardening；
(3) the agricultural microorganism bacterial manure that the present invention is provided can be made in soil with chelate presence with fixed nitrogen, phosphorus decomposing, potassium decomposing
Nitrogen, phosphorus, potassium effectively discharged；
(4) in the agricultural microorganism bacterial manure that the present invention is provided：Bacillus subtilis can increase crop anti-adversity, fixed nitrogen；
Bacillus megaterium can be with phosphorus decomposing (phosphobacteria), organophosphor in the soil that has functions that to degrade well；Bacillusmusilaginosiengineering
The middle trace element such as solvable phosphorus potassium element and calcium, sulphur, magnesium, iron, zinc, molybdenum, manganese can be discharged with potassium decomposing；Bacillus licheniformis can
With disease-resistant, killing harmful bacteria；Bacillus thuringiensis can have spy with desinsection (including root-knot nematode) to arthropods such as Lepidopteras
The cytotoxicity of the opposite sex；Bacillus laterosporus can be with promoting root growth, sterilized and degraded heavy metal；Colloid bacillus cereus can have Soluble phosphorus,
Potassium release and fixed nitrogen function, secrete various enzymes, resistance of the enhancing crop to some diseases；Streptomyces jingyangensis can have enhancing soil
Earth fertility, the ability for stimulating plant growth；The nomadic nitrogen that azotobacter vinelandii can be fixed in air, volume increase；Bacillus coagulans
The pernicious gases such as ammonia, hydrogen sulfide in environment can be reduced.Improve the content of amino acid in fruit；Aspergillus oryzae can make in stalk
Organic matter turn into plant growth needed for nutrition, improve the soil organism, improve soil texture；Paecilomyces lilacinus：To various
Nematode prevents and treats efficiency, is the preventing and treating most promising biological prevention and control agent of root-knot nematode；
(5) the agricultural microorganism bacterial manure that the present invention is provided, is the demand for combining market, and breach is done from microorganism field,
Combined in terms of organic matter and trace element, water-solubleization of microorganism and organic matter and trace element is realized comprehensively, so that native
Earth obtains nutritional supplementation, root system of plant is obtained green, organic, environmentally friendly guarantee, be long-term use chemical fertilizer it is hardened, acidifying,
Alkali-affected soil is effectively supplemented, and gradually improved soil nutrition can be constructed, and the crop for eating our mankind is more healthy,
More ecosystem, it may be said that be the most basic method for solving food-safety problem, is also more advanced and science method.
Additional aspect of the invention and advantage will be set forth in part in the description, and will partly become from the following description
Obtain substantially, or recognized by practice of the invention.
Specific embodiment
Below in conjunction with the embodiment of the present invention, technical scheme is clearly and completely described.Hereinafter implement
Example is only used for clearly illustrating technical scheme, therefore is intended only as example, and can not limit this hair with this
Bright protection domain.
Experimental technique in following embodiments, unless otherwise specified, is conventional method.Examination used in following embodiments
Material is tested, unless otherwise specified, is what is be commercially available from routine biochemistry reagent shop.Quantitative test in following examples,
It is respectively provided with three repetitions to test, data are the average value or mean+SD of three repetition experiments.
Embodiment one
The present embodiment provides a kind of agricultural microorganism bacterial manure and preparation method thereof.
1st, nutrient medium culture bacillus licheniformis and bacillus subtilis is respectively adopted；With the total of the culture medium
On the basis of weight, the percentage composition of peptone is that the percentage composition of 0.5%, NaCl can be 0.5%, beef extract in the culture medium
Percentage composition can be 0.2%, balance of sterilized water, the pH value of the culture medium is 7.0；Cultural method is cellar culture side
Method, culture are until the viable count of bacillus licheniformis is (1~10) × 1010CFU/g, the viable count of bacillus subtilis for (1~
10)×1010CFU/g。
2nd, using YPG medium culture bacillus coagulanses；On the basis of the gross weight of the culture medium, egg in the culture medium
The percentage composition of peptone is that the percentage composition that the percentage composition of 0.25%, glucose is 0.25%, yeast extract is 0.2%, surplus in vain
It is sterilized water, the pH value of the culture medium is 7.2；Cultural method is conventional culture methods, and culture is until the work of bacillus coagulans
Bacterium number is (1~10) × 1010CFU/g。
3rd, a medium culture Streptomyces jingyangensis, on the basis of the gross weight of the culture medium, the training are synthesized using Gao Shi
The percentage composition for supporting soluble starch in base is 5%, KNO3Percentage composition be 0.1%, K2HPO4Percentage composition be
The percentage composition of 0.25%, NaCl is 0.05%, balance of sterilized water, and the pH value of the culture medium is 7.2；Cultural method is conventional
Cultural method, culture are until the viable count of Streptomyces jingyangensis is (1~10) × 1010CFU/g。
4th, culture bacillus megaterium, colloid bacillus cereus, bacillusmusilaginosiengineering, the culture of azotobacter vinelandii respectively
Base, every 100g culture mediums used include：Sucrose 2g, mannitol 5g, ammonium sulfate 1g, peptone 1g, beef extract 2g, sodium chloride
1g, potassium dihydrogen phosphate 0.15g, ammonium molybdate 0.6g, calcium carbonate 0.02g, frerrous chloride 0.02g, magnesium sulfate 0.02g, balance of water,
Regulation pH value is 7.3；Cultural method is conventional culture methods, culture until the viable count of bacillus megaterium is (1~10)
×1010CFU/g, the viable count of colloid bacillus cereus is (1~10) × 1010CFU/g, the viable count of bacillusmusilaginosiengineering is
(1~10) × 1010CFU/g, the viable count of azotobacter vinelandii is (1~10) × 1010CFU/g。
5th, culture culture bacillus thuringiensis, in the culture medium of every 100g used, starch 3.0g, beancake powder 1.8g,
The sub- powder 1.2g of cottonseed cake, peptone 0.5g, dusty yeast 0.4g, corn pulp 2.0g, epsom salt 0.04g, potassium dihydrogen phosphate 0.2g,
The 1/400 of dipotassium hydrogen phosphate 0.2g, calcium carbonate 0.1g and AMS/content of starch, balance of water, the pH value of the culture medium
It is 7.2；Cultural method is conventional culture methods, and culture is until the viable count of bacillus thuringiensis is (1~10) × 1010CFU/
g。
6th, bacillus laterosporus is cultivated, in the culture medium of every 100g used, beef extract 0.5g, peptone 1g, sodium chloride
0.5g, balance of water, pH value is 7.3；Cultural method is conventional culture methods, and culture is until the viable count of bacillus laterosporus is
(1~10) × 1010CFU/g。
7th, aspergillus oryzae is cultivated, in the culture medium of every 100g used, glucose 0.5g, soya-bean milk 30g, corn pulp 0.2g, sulphur
Sour magnesium 0.05g, dipotassium hydrogen phosphate 0.2g, balance of water, the pH value of the culture medium is 7.3；Cultural method is conventional culture methods,
Culture is until the viable count of aspergillus oryzae is (1~10) × 1010CFU/g。
8th, Paecilomyces lilacinus is cultivated, in the culture medium of every 1000g used, NaNO3 3g、K2HPO4 1g、KCl 0.5g、
MgSO4·7H2O 0.5g、FeSO40.01g, sucrose 30g, balance of water, the pH value of the culture medium is 7.3；Cultural method is normal
Rule cultural method, culture are until the viable count of Paecilomyces lilacinus is (1~10) × 1010CFU/g。
By above-mentioned bacillus subtilis, bacillus megaterium, bacillusmusilaginosiengineering, the lichens independently cultivated and obtain
Bacillus, bacillus thuringiensis, bacillus laterosporus, colloid bacillus cereus, Streptomyces jingyangensis, azotobacter vinelandii, condensation
Bacillus, aspergillus oryzae and Paecilomyces lilacinus liquid determine viable count respectively, each in last agricultural microorganism bacterial manure
The viable count content of strain, by calculating, is mixed (can finally be quantified using water) in appropriate proportion.The present embodiment
In the agricultural microorganism bacterial manure being mixed to get, by determining, shown in the viable count table 1 specific as follows of different strains：
The viable count of each strain in the agricultural microorganism bacterial manure of table 1
| Strain name | Latin title | The viable count of each strain |
| Bacillus subtilis | Bacillus subtilis | |
| Bacillus megaterium | Bacillus megaterium | |
| Bacillusmusilaginosiengineering | Bacillus mucilaginosus | |
| Bacillus licheniformis | Bacillus licheniformis | |
| Bacillus thuringiensis | Bacillus thuringiensis | |
| Bacillus laterosporus | Bacillus laterosporus | |
| Colloid bacillus cereus | Bacillus mucilaginosus | |
| Streptomyces jingyangensis | Streptomyces jingyangensis | |
| Azotobacter vinelandii | Azotobactervinelandii | |
| Bacillus coagulans | Bacillus coagulans | |
| Aspergillus oryzae | Aspergillus oryzae | |
| Paecilomyces lilacinus | Paecilomyces lilacinus |
Embodiment two
The present embodiment is used to illustrate that the agricultural microorganism bacterial manure of present invention offer to be used for getting fat method and its getting fat of soil
Effect.The soil that the present embodiment is selected is paddy rice soil property, and experiment place is in the good garden agricultural hairs of Growth in Meizhou City, Guangdong Mei County Nan Kouzhen
Exhibition Co., Ltd, the plant of experiment is four or nine cabbage hearts, and experimental unit is the experiment of arable land fertilizer master station of commission Guangdong Province.
Experimental technique：Wherein experimental group：The agricultural microorganism bacterial manure that embodiment one is prepared is inoculated in soil, agriculture
It is 0.2 with the mass ratio of microbial-bacterial fertilizer and the soil:100, uniform after fermentation is turned, the temperature of fermentation is 20 DEG C, fermentation
Time is 5 days, obtains getting fat soil.Then four or nine cabbage hearts are planted in getting fat soil, cultivated area is 5 mu of ground, implantation methods
With conventional implantation methods and the management method in field.
In order to preferably characterize the effect of the agricultural microorganism bacterial manure that the present invention is prepared, three controls are also provided with
Group, respectively control group one, control group two and control group three.
Wherein, control group one is that the agricultural microorganism bacterial manure for preparing the embodiment of the present invention one carries out inactivation treatment,
Then it is inoculated in soil, the mass ratio of agricultural microorganism bacterial manure and the soil is 0.2:100, uniform after fermentation is turned, send out
The temperature of ferment is 20 DEG C, and fermentation time is 5 days, soil after being processed.Then four or nine cabbage hearts are planted in soil after treatment, is planted
Plant area is 5 mu of ground, implantation methods and the same experimental group of management method.
Control group two is that commercially available microbial-bacterial fertilizer is inoculated in soil, the matter of agricultural microorganism bacterial manure and the soil
Amount is than being 0.2:100, uniform after fermentation is turned, the temperature of fermentation is 20 DEG C, and fermentation time is 5 days, soil after being processed.So
Plant four or nine cabbage hearts in soil after treatment afterwards, cultivated area is 5 mu of ground, implantation methods and the same experimental group of management method.
Control group three is not apply fertilizer, and after soil is turned uniformly, is maintained 5 days at 20 DEG C, soil after being processed.Then exist
Four or nine cabbage hearts are planted after treatment in soil, cultivated area is 5 mu of ground, implantation methods and the same experimental group of management method.
Experimental result：The concrete outcome of the yield for obtaining is as shown in table 2 below.
The Yield comparison of four or nine cabbage hearts of the different groups of table 2
| Contrast groups | Experimental group is than control group one | Experimental group is than control group two | Experimental group is than control group three |
| Rate of growth | 9.2% | 11.1% | 60.7% |
Agricultural microorganism bacterial manure that the present invention is provided and preparation method and application, with following beneficial effect：(1) originally
The agricultural microorganism bacterial manure that invention is provided fundamentally solves conventional fertilizers and only promotes plant growth, is only sought to soil absorption
Support, and cannot for and soil nutrient shortcoming；(2) the agricultural microorganism bacterial manure that the present invention is provided can improve any soil,
Can be supplemented the nutrients to soil with effectively preventing part pest and disease damage, effectively to solve the long-standing problem of soil hardening；
(3) present invention provide agricultural microorganism bacterial manure can with fixed nitrogen, phosphorus decomposing, potassium decomposing, make in soil with chelate exist nitrogen, phosphorus,
Potassium is effectively discharged；(4) in the agricultural microorganism bacterial manure that the present invention is provided：It is degeneration-resistant that bacillus subtilis can increase crop
Property, fixed nitrogen；Bacillus megaterium can be with phosphorus decomposing (phosphobacteria), organophosphor in the soil that has functions that to degrade well；Gel-shaped
Bacillus can discharge the middle trace element such as solvable phosphorus potassium element and calcium, sulphur, magnesium, iron, zinc, molybdenum, manganese with potassium decomposing；Lichens bud
Spore bacillus can be with disease-resistant, killing harmful bacteria；Bacillus thuringiensis can be with desinsection (including root-knot nematode), to segmental appendages such as Lepidopteras
Animal has specific cytotoxicity；Bacillus laterosporus can be with promoting root growth, sterilized and degraded heavy metal；Colloid bacillus cereus can be with
There are Soluble phosphorus, Potassium release and fixed nitrogen function, secrete various enzymes, resistance of the enhancing crop to some diseases；Streptomyces jingyangensis can have
There is enhancing soil fertility, stimulate the ability of plant growth；The nomadic nitrogen that azotobacter vinelandii can be fixed in air, volume increase；Condense
Bacillus can reduce the pernicious gases such as ammonia, the hydrogen sulfide in environment.Improve the content of amino acid in fruit；Aspergillus oryzae can be with
Nutrition needed for making the organic matter in stalk turn into plant growth, improves the soil organism, improves soil texture；It is pale purple to intend blue or green
It is mould：Efficiency is prevented and treated to various nematodes, is the preventing and treating most promising biological prevention and control agent of root-knot nematode；(5) agriculture that the present invention is provided
It is the demand for combining market with microbial-bacterial fertilizer, breach is done from microorganism field, is tied in terms of organic matter and trace element
Close, water-solubleization of microorganism and organic matter and trace element is realized comprehensively, so that soil obtains nutritional supplementation, make root system of plant
Obtain green, organic, environmentally friendly guarantee, be long-term use chemical fertilizer it is hardened, acidifying, alkali-affected soil effectively supplemented, can
Constructed with gradually improved soil nutrition, the crop for eating our mankind is more healthy, more ecosystem, it may be said that be to solve food
The most basic method of product safety problem, is also more advanced and science method.
It should be noted that unless otherwise indicated, technical term used in this application or scientific terminology should be this hair
The ordinary meaning that bright one of ordinary skill in the art are understood.Unless specifically stated otherwise, otherwise illustrate in these embodiments
The relative step of part and step, numerical expression and numerical value do not limit the scope of the invention.It is illustrated and described herein
In all examples, unless otherwise prescribed, any occurrence should be construed as merely exemplary, not as limitation, because
This, other examples of exemplary embodiment can have different values.
Finally it should be noted that：Various embodiments above is merely illustrative of the technical solution of the present invention, rather than its limitations；To the greatest extent
Pipe has been described in detail with reference to foregoing embodiments to the present invention, it will be understood by those within the art that：Its according to
The technical scheme described in foregoing embodiments can so be modified, or which part or all technical characteristic are entered
Row equivalent；And these modifications or replacement, the essence of appropriate technical solution is departed from various embodiments of the present invention technology
The scope of scheme, it all should cover in the middle of protection scope of the present invention.
Claims (10)
1. a kind of agricultural microorganism bacterial manure, it is characterised in that including culture medium and thalline, the thalline includes：
Bacillus subtilis (Bacillus subtilis), bacillus megaterium (Bacillus megaterium), gel-shaped
Bacillus (Bacillus mucilaginosus), bacillus licheniformis (Bacillus licheniformis), Su Yunjin
Bacillus (Bacillus thuringiensis), bacillus laterosporus (Bacillus laterosporus), colloid gemma
Bacillus (Bacillus mucilaginosus), Streptomyces jingyangensis (Streptomyces jingyangensis), brown fixed nitrogen
Bacterium (Azotobactervinelandii), bacillus coagulans (Bacillus coagulans), aspergillus oryzae (Aspergillus
) and Paecilomyces lilacinus (Paecilomyces lilacinus) oryzae.
2. agricultural microorganism bacterial manure according to claim 1, it is characterised in that：
In the agricultural microorganism bacterial manure, the viable count of bacillus subtilis is (0.5~5) × 108CFU/g, huge gemma bar
The viable count of bacterium is (0.5~5) × 108CFU/g, the viable count of bacillusmusilaginosiengineering is (0.5~5) × 108CFU/g, lichens
The viable count of bacillus is (1~3) × 108CFU/g, the viable count of bacillus thuringiensis is (1~5) × 108CFU/g, side
The viable count of spore bacillus is (1~3) × 108CFU/g, the viable count of colloid bacillus cereus is (1~3) × 108CFU/g, river rising in Ningxia and flowing into central Shaanxi
The viable count of positive streptomycete is (1~3) × 108CFU/g, the viable count of azotobacter vinelandii is (1~3) × 108CFU/g, condenses bud
The viable count of spore bacillus is (1~3) × 108CFU/g, the viable count of aspergillus oryzae is (1~5) × 108CFU/g, Paecilomyces lilacinus
Viable count is (1~3) × 108CFU/g。
3. the preparation method of the agricultural microorganism bacterial manure described in claim 2, it is characterised in that comprise the following steps：
By bacillus subtilis, bacillus megaterium, bacillusmusilaginosiengineering, bacillus licheniformis, bacillus thuringiensis, side
Spore bacillus, colloid bacillus cereus, Streptomyces jingyangensis, azotobacter vinelandii, bacillus coagulans, aspergillus oryzae and Paecilomyces lilacinus
It is inoculated in culture medium and is cultivated, the viable count for making bacillus subtilis is (0.5~5) × 108CFU/g, huge gemma bar
The viable count of bacterium is (0.5~5) × 108CFU/g, the viable count of bacillusmusilaginosiengineering is (0.5~5) × 108CFU/g, lichens
The viable count of bacillus is (1~3) × 108CFU/g, the viable count of bacillus thuringiensis is (1~5) × 108CFU/g, side
The viable count of spore bacillus is (1~3) × 108CFU/g, the viable count of colloid bacillus cereus is (1~3) × 108CFU/g, river rising in Ningxia and flowing into central Shaanxi
The viable count of positive streptomycete is (1~3) × 108CFU/g, the viable count of azotobacter vinelandii is (1~3) × 108CFU/g, condenses bud
The viable count of spore bacillus is (1~3) × 108CFU/g, the viable count of aspergillus oryzae is (1~5) × 108CFU/g, Paecilomyces lilacinus
Viable count is (1~3) × 108CFU/g。
4. the preparation method of the agricultural microorganism bacterial manure described in claim 2, it is characterised in that comprise the following steps：
Cultivated respectively in each independent cultivating system bacillus subtilis, bacillus megaterium, bacillusmusilaginosiengineering,
It is clothing bacillus, bacillus thuringiensis, bacillus laterosporus, colloid bacillus cereus, Streptomyces jingyangensis, azotobacter vinelandii, solidifying
Knot bacillus, aspergillus oryzae and Paecilomyces lilacinus；And will respectively cultivate bacillus subtilis, bacillus megaterium, the glue for obtaining
Freeze sample bacillus, bacillus licheniformis, bacillus thuringiensis, bacillus laterosporus, colloid bacillus cereus, Jingyang strepto-
Bacterium, azotobacter vinelandii, bacillus coagulans, aspergillus oryzae and Paecilomyces lilacinus are proportionally mixed, so as to get it is agricultural micro-
In bio-bacterial manure：The viable count of bacillus subtilis is (0.5~5) × 108CFU/g, the viable count of bacillus megaterium is
(0.5~5) × 108CFU/g, the viable count of bacillusmusilaginosiengineering is (0.5~5) × 108CFU/g, the work of bacillus licheniformis
Bacterium number is (1~3) × 108CFU/g, the viable count of bacillus thuringiensis is (1~5) × 108CFU/g, bacillus laterosporus
Viable count is (1~3) × 108CFU/g, the viable count of colloid bacillus cereus is (1~3) × 108CFU/g, the work of Streptomyces jingyangensis
Bacterium number is (1~3) × 108CFU/g, the viable count of azotobacter vinelandii is (1~3) × 108CFU/g, the viable bacteria of bacillus coagulans
Number is (1~3) × 108CFU/g, the viable count of aspergillus oryzae is (1~5) × 108CFU/g, the viable count of Paecilomyces lilacinus for (1~
3)×108CFU/g。
5. a kind of method of soil enrichment, it is characterised in that comprise the following steps：
Agricultural microorganism bacterial manure described in claim 1 or 2 is inoculated in soil, uniform after fermentation is turned, getting fat soil is obtained
Earth.
6. the method for soil enrichment according to claim 5, it is characterised in that：
The mass ratio of the agricultural microorganism bacterial manure and the soil is (0.05~0.2):100.
7. the method for soil enrichment according to claim 5, it is characterised in that：
The temperature of the fermentation is 20~30 DEG C.
8. the method for soil enrichment according to claim 5, it is characterised in that：
The fermentation time is 3~5 days.
9. the agricultural microorganism bacterial manure described in claim 1 or 2 is in the soil used by getting fat planting vegetable, fruit tree, paddy rice, corn
In application.
10. application according to claim 9, it is characterised in that：
The vegetables are cabbage heart, and the cabbage heart is four or nine cabbage hearts.
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| CN113789181B (en) * | 2021-10-29 | 2024-02-13 | 广东华茂水电生态集团有限公司 | Soil conditioner and preparation method thereof |
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