CN110268917A - Applying Ectomycorrhizal Fungi composite bacteria agent - Google Patents
Applying Ectomycorrhizal Fungi composite bacteria agent Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/10—Mycorrhiza; Mycorrhizal associations
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The invention discloses a kind of Applying Ectomycorrhizal Fungi composite bacteria agents, including compound Applying Ectomycorrhizal Fungi 4-8 parts, phosphorus decomposing, 2-6 parts of B. mucilaginocus, 2-6 parts of compound fungus, 20-30 parts of rice straw powder, 20-30 parts of coconut palm suede, 5-10 parts of ammonium molybdate, 2-6 parts of boric acid, 3-8 parts of polyvinyl alcohol, 10-15 parts of hydroxypropyl-β-cyclodextrin, 10-15 parts of chitosan, -5 parts of growth hormone 1,10-20 parts of olive pomace；The compound Applying Ectomycorrhizal Fungi is the mixture of colored acne Lasiosphaera fenzlii, delicious lactarius and suillusbovinus, and the phosphorus decomposing, B. mucilaginocus are bacillus megaterium and colloid bacillus cereus, and the compound fungus is the mixture of long handle trichoderma and Streptomyces jingyangensis；A variety of microbial inoculum synergistic effects, play group's joint effect, ultimately form a complicated composition, stable structure, the extensive microbiologic population of function, can promote plant and root growth, improve nutrient and moisture absorption, the diversification that same host plant is infected from different bacterium is realized, the nutrient absorption of host plant has obtained very big reinforcement, and biomass is significantly improved.
Description
Technical field
The present invention relates to a kind of composite bacteria agent, in particular to a kind of ectotrophic mycorrhiza composite bacteria agent for breeding and afforestation.
Background technique
Each process of the ecosystem along with various microorganisms activity, most important one functional group first is that bacterium
Mycorrhiza fungi (VA Mycorrhizal Fungi).VA Mycorrhizal Fungi is that nature most plants are survived most basic component part, land about 90% or more
Higher plant can form mycorhiza, wherein 73.9% xylophyta can form exotrophic mycorrhiza, Applying Ectomycorrhizal Fungi can pass through
Absorption, the fixing effect of itself, reduce heavy metal-polluted soil to the validity and toxicity of plant.Meanwhile Applying Ectomycorrhizal Fungi is also
Beneficial to improving arboreal growth situation, enhances its anti-(resistance to) heavy metal ability, improve the afforestation survival under the conditions of heavy metal stress
Rate.The mycelium of these mycorrhizal fungis forms mycorhiza in conjunction with root system of plant, promotes plant growth, while can form huge bacterium
Wire network makes variety classes root system of plant transmit information and mass exchange by mycelia network.The huge mycelia spread in soil
Body is one of main absorption organ of mycorrhizal plants, and after plant forms mycorhiza, absorption root density be will increase, and Root morphology changes,
The inaccessiable soil of former root system of plant can be deep into, in rock fine crack, largely expand the suction of plant moisture, nutrient
It receives range (especially to the absorption of P elements and utilization), to improve the biomass of plant.Mycorrhizal fungi can also activating soil
In mineral element, promote absorption of the root system of plant to soil mineral nutrient, especially to mineral matter elements such as zinc and copper
Absorption.The formation of mycorhiza can reduce the permanent wilting point of plant, improve plant drought resistance and water use efficiency.In addition, right
Forest ectomycorrhizal fungi-inoculated, can also enhance its resistance in adverse circumstance environment, improve plant percent, enhancing impoverishment tolerant,
Cold-resistant, saline-alkali tolerant ability, can also greatly reduce plant disease, especially soil-borne disease fungal pathogens and cyst nematode, root-knot nematode
Equal insect pest of the plant.Mycorrhizal fungi can the outer glycoprotein of secretory cell be conducive to the shape of soil aggregate structure and in conjunction with mycelia net
At soil stability can be improved, keep soil porosity, guarantee good tillage condition and aeration status, in fixing soil
Organic matter improves the carbon deposit in soil.Exotrophic mycorrhiza mycorhiza also can reduce by heavy metal, organic pollutant, radioactive element
It is adversely affected Deng the pollution caused by soil environment and caused by nursery stock, using the plant of Arbuscular Mycorrhizal Fungi than non-Arbuscular Mycorrhizal Fungi
Plant can absorb more heavy metals and organic pollutant without being poisoned, small molecular organic acid (oxalic acid, lemon of secretion
Acid etc.) complexing pollutant, to weaken its toxicity, while reducing transport of the pollutant from root to overground part, is solid in soil
The important bunker for determining pollutant has huge value in contaminant degradation.Existing mycorhiza agent ingredient is single, therefore function
It can single, bad adaptability.
Summary of the invention
In view of this, a variety of microbial inoculum collaborations are made the purpose of the present invention is to provide a kind of Applying Ectomycorrhizal Fungi composite bacteria agent
With performance group's joint effect ultimately forms a complicated composition, stable structure, the extensive microbiologic population of function can promote
Plant and root growth improve nutrient and moisture absorption, realize the diversification that same host plant is infected from different bacterium, host
The nutrient absorption of plant has obtained very big reinforcement, and biomass is significantly improved.
Applying Ectomycorrhizal Fungi composite bacteria agent of the invention, raw material includes following components by weight: compound mycorrhizal fungi 4-8
Part, 2-6 parts of dissolving phosphor and dissolving potassium bacterium, 2-6 parts of compound fungus, 20-30 parts of rice straw powder, 20-30 parts of coconut palm suede, 5-10 parts of ammonium molybdate, boron
2-6 parts sour, 3-8 parts of polyvinyl alcohol, 10-15 parts of hydroxypropyl-β-cyclodextrin, 10-15 parts of chitosan, -5 parts of growth hormone 1, oil
10-20 parts of olive pomace；The compound Applying Ectomycorrhizal Fungi is the mixture of colored acne Lasiosphaera fenzlii, delicious lactarius and suillusbovinus,
The phosphorus decomposing, B. mucilaginocus be bacillus megaterium and colloid bacillus cereus mixture, the compound fungus be long handle trichoderma and
The mixture of Streptomyces jingyangensis；
Further, raw material includes following components by weight: 6 parts of compound exotrophic mycorrhiza mycorrhizal fungi, phosphorus decomposing B. mucilaginocus 4
Part, 4 parts of compound fungus, 25 parts of rice straw powder, 25 parts of coconut palm suede, 8 parts of ammonium molybdate, 4 parts of boric acid, 6 parts of polyvinyl alcohol, hydroxy propyl-Beta-ring
12 parts of dextrin, 12 parts of chitosan, 3 parts of growth hormone, 15 parts of olive pomace；
Further, acne Lasiosphaera fenzlii colored in mass ratio: delicious lactarius: suillusbovinus=1:1:1；
Further, by quality bacillus megaterium: colloid bacillus cereus=1:2；；
Further, long handle trichoderma in mass ratio: Streptomyces jingyangensis=2:1；
Further, the growth hormone is the mixture of methyl α-naphthyl acetate and indole-3-acetic acid；
Further, methyl α-naphthyl acetate in mass ratio: indole-3-acetic acid=2:1；
Further, the chitosan has three-dimensional porous reticular structure；
Further, above-mentioned raw materials are uniformly mixed to prepare to VA Mycorrhizal Fungi composite bacteria agent.
Beneficial effects of the present invention: ectotrophic mycorrhiza composite bacteria agent of the invention, a variety of microbial inoculum synergistic effects play group
Joint effect, ultimately forms a complicated composition, stable structure, and the extensive microbiologic population of function can promote plant and root system
Growth improves nutrient and moisture absorption, realizes the diversification that same host plant is infected from different bacterium, the nutrition of host plant
Absorption has obtained very big reinforcement, and biomass is significantly improved.By huge mycelium, it can promote root system of plant and absorb acquisition
More nutrients and moisture；It can promote absorption of the plant to inorganic organic minerals by enzyme；Antibiotic is secreted, is improved
The resistivity of plant for disease improves soil physico-chemical characteristic, enhances the biologos of soil system.
Specific embodiment
The exomycorhiza bacterium composite bacteria agent of the present embodiment, raw material includes following components by weight: compound Applying Ectomycorrhizal Fungi
4-8 parts, phosphorus decomposing, 2-6 parts of B. mucilaginocus, 2-6 parts of compound fungus, 20-30 parts of rice straw powder, 20-30 parts of coconut palm suede, 5-10 parts of ammonium molybdate,
2-6 parts of boric acid, 3-8 parts of polyvinyl alcohol, 10-15 parts of hydroxypropyl-β-cyclodextrin, 10-15 parts of chitosan, -5 parts of growth hormone 1, oil
10-20 parts of olive pomace；The ectotrophic mycorrhiza composite bacteria agent is colored acne Lasiosphaera fenzlii: delicious lactarius: the mixing of suillusbovinus
Object, the dissolving phosphor and dissolving potassium bacterium are the mixture of bacillus megaterium and colloid bacillus cereus.The compound fungus is
The mixture of long handle trichoderma and Streptomyces jingyangensis；It is multiple without antagonism between compound mycorrhizal fungi, dissolving phosphor and dissolving potassium bacterium and compound fungus
Closing has interaction between Applying Ectomycorrhizal Fungi and compound fungus, the specificity with height can cooperate with improvement plant nutrient
And growth effects, promote plant growth, enhance biocontrol effect, cooperate with rehabilitating soil, activating soil nutrient promotes plant nutrient to inhale
It receives and utilizes；In compound fungus, long handle trichoderma and Streptomyces jingyangensis are without mutual inhibiting effect, compatibility with higher, and energy
Synergy promotes the morphogenesis of root and overground part, promotes the growth of root system and stem, leaf, dramatically increases underground part root system body
Product, root system total surface area, root long and root average diameter and overground part stem height, stem girth, leaf length, leaf width and biomass, have
Significant growth-promoting efficiency；Colored acne Lasiosphaera fenzlii in compound Applying Ectomycorrhizal Fungi: delicious lactarius: suillusbovinus mutually without antagonism, and
There is synergy.It is acted synergistically using ammonium molybdate, boric acid and compound mycorrhizal fungi, the viable count of generation is high, high viable bacteria
Number duration is long；Coconut palm suede, rice straw powder and chitosan work and hydroxypropyl-β-cyclodextrin with three-dimensional porous reticular structure
Enough growing spaces are provided as multi-faceted, the absorption of solid space and coated carrier, and to effective bacterium, promote effective viable bacteria
Several growths improves survival rate.
In the present embodiment, raw material includes following components by weight: 6 parts of compound mycorrhizal fungi, is answered at 4 parts of phosphorus decomposing B. mucilaginocus
Close 4 parts of mould, 25 parts of rice straw powder, 25 parts of coconut palm suede, 8 parts of ammonium molybdate, 4 parts of boric acid, 6 parts of polyvinyl alcohol, hydroxypropyl-β-cyclodextrin 12
Part, 12 parts of chitosan, 3 parts of growth hormone, 15 parts of olive pomace；For preferred embodiment.
In the present embodiment, acne Lasiosphaera fenzlii colored in mass ratio: delicious lactarius: suillusbovinus=1:1:1；Compound mycorrhizal fungi it
Between ratio decide its interaction effect.
In the present embodiment, by quality bacillus megaterium: colloid bacillus cereus=1:2, the ratio between bacterium decide
Its effect to interact.
In the present embodiment, long handle trichoderma and Streptomyces jingyangensis 2:1 in mass ratio；Ratio between mould decides it mutually
The effect of effect.
In the present embodiment, the growth hormone is the mixture of methyl α-naphthyl acetate and indole-3-acetic acid；Methyl α-naphthyl acetate in mass ratio:
Indole-3-acetic acid=2:1；The ingredient and proportion of growth hormone decide growth and the time-to-live of effective bacterium.
In the present embodiment, the chitosan has three-dimensional porous reticular structure；Multi-azimuth tridimensional absorption is carried out, while being given again
Effective bacterium retains enough growing spaces.
In the present embodiment, above-mentioned raw materials are uniformly mixed to prepare to VA Mycorrhizal Fungi composite bacteria agent；It is simple to operate.
Embodiment one
The VA Mycorrhizal Fungi composite bacteria agent of the present embodiment, raw material includes following components by weight: compound Applying Ectomycorrhizal Fungi 4
Part, phosphorus decomposing, 2 parts of B. mucilaginocus, 2 parts of compound fungus, 20 parts of rice straw powder, 20 parts of coconut palm suede, 5 parts of ammonium molybdate, 2 parts of boric acid, polyvinyl alcohol 3
Part, 10 parts of hydroxypropyl-β-cyclodextrin, 10 parts of chitosan, growth hormone 1 part, 10 parts of olive pomace.
Embodiment two
The ectotrophic mycorrhiza composite bacteria agent of the present embodiment, raw material includes following components by weight: compound mycorrhizal fungi 8
Part, phosphorus decomposing, 6 parts of B. mucilaginocus, 6 parts of compound fungus, 30 parts of rice straw powder, 30 parts of coconut palm suede, 10 parts of ammonium molybdate, 6 parts of boric acid, polyvinyl alcohol
8 parts, 15 parts of hydroxypropyl-β-cyclodextrin, 15 parts of chitosan, 5 parts of growth hormone, 20 parts of olive pomace.
Embodiment three
The ectotrophic mycorrhiza composite bacteria agent of the present embodiment, raw material includes following components by weight: compound exotrophic mycorrhiza is true
8 parts of bacterium, phosphorus decomposing, 2 parts of B. mucilaginocus, 6 parts of compound fungus, 20 parts of rice straw powder, 30 parts of coconut palm suede, 5 parts of ammonium molybdate, 2 parts of boric acid, polyethylene
8 parts of alcohol, 10 parts of hydroxypropyl-β-cyclodextrin, 15 parts of chitosan, growth hormone 1 part, 20 parts of olive pomace.
Example IV
The ectotrophic mycorrhiza composite bacteria agent of the present embodiment, raw material includes following components by weight: compound exotrophic mycorrhiza is true
8 parts of bacterium, phosphorus decomposing, 2 parts of B. mucilaginocus, 6 parts of compound fungus, 20 parts of rice straw powder, 30 parts of coconut palm suede, 5 parts of ammonium molybdate, 6 parts of boric acid, polyethylene
3 parts of alcohol, 15 parts of hydroxypropyl-β-cyclodextrin, 10 parts of chitosan, 5 parts of growth hormone, 10 parts of olive pomace.
Embodiment five
The ectotrophic mycorrhiza composite bacteria agent of the present embodiment, raw material includes following components by weight: compound exotrophic mycorrhiza is true
6 parts of bacterium, 2 parts of phosphorus decomposing B. mucilaginocus, 6 parts of compound fungus, 25 parts of rice straw powder, 30 parts of coconut palm suede, 5 parts of ammonium molybdate, 4 parts of boric acid, polyethylene
3 parts of alcohol, 15 parts of hydroxypropyl-β-cyclodextrin, 12 parts of chitosan, 3 parts of growth hormone, 15 parts of olive pomace.
Embodiment six
The ectotrophic mycorrhiza composite bacteria agent of the present embodiment, raw material includes following components by weight: compound exotrophic mycorrhiza is true
6 parts of bacterium, phosphorus decomposing, 4 parts of B. mucilaginocus, 4 parts of compound fungus, 25 parts of rice straw powder, 25 parts of coconut palm suede, 8 parts of ammonium molybdate, 4 parts of boric acid, polyethylene
6 parts of alcohol, 12 parts of hydroxypropyl-β-cyclodextrin, 12 parts of chitosan, 3 parts of growth hormone, 15 parts of olive pomace.
In above-described embodiment, acne Lasiosphaera fenzlii colored in mass ratio: delicious lactarius: suillusbovinus Pseudomonas aurantica: solution starch
Bacillus: bacillus megaterium=1::1:1；By quality bacillus megaterium: colloid bacillus cereus=1:2；It is long in mass ratio
Handle trichoderma and river rising in Ningxia and flowing into central Shaanxi, positive streptomycete=2::1；；The growth hormone is the mixture of methyl α-naphthyl acetate and indole-3-acetic acid；In mass ratio
Methyl α-naphthyl acetate: indole-3-acetic acid=2:1；The chitosan has three-dimensional porous reticular structure；It is used in above-described embodiment
Each bacterium during compound Applying Ectomycorrhizal Fungi, phosphorus decomposing, B. mucilaginocus, compound fungus are equal can directly buy from the market, then
VA Mycorrhizal Fungi composite bacteria agent is uniformly mixed to prepare with other raw materials.
It will the VA Mycorrhizal Fungi composite bacteria agent of above-described embodiment one and pouring or spraying masson pine after water 1:50 dilution by volume
Seedling root, as a result as follows:
The horse hair after transplanting will be poured after the 1:30 dilution by volume of the VA Mycorrhizal Fungi composite bacteria agent of above-described embodiment and water
The root Song Miao, as a result as follows:
| Processing | Survival rate | Height of seedling (m) | Root diameter ratio | Mycorhiza Infection Rate % |
| Embodiment 1 | 96.19 | 3.15a | 2.15a | 81.13a |
| Embodiment 2 | 95.98 | 3.33a | 1.89a | 84.21a |
| Embodiment 3 | 96.67 | 3.21a | 1.94a | 82.15a |
| Embodiment 4 | 95.89 | 3.08a | 2.01a | 83.68a |
| Embodiment 5 | 96.68 | 3.23a | 1.98a | 80.98a |
| Embodiment 6 | 97.54 | 3.5a | 2.11a | 84.35a |
| Clear water (control group) | 64.2 | 1.57c | 0.69c | 10.56c |
Finally, it is stated that the above examples are only used to illustrate the technical scheme of the present invention and are not limiting, although referring to compared with
Good embodiment describes the invention in detail, those skilled in the art should understand that, it can be to skill of the invention
Art scheme is modified or replaced equivalently, and without departing from the objective and range of technical solution of the present invention, should all be covered at this
In the scope of the claims of invention.
Claims (9)
1. a kind of Applying Ectomycorrhizal Fungi composite bacteria agent, it is characterised in that: raw material includes following components by weight: compound external bacterium
4-8 parts of mycorrhiza fungi, 2-6 parts of dissolving phosphor and dissolving potassium bacterium, 2-6 parts of compound fungus, 20-30 parts of rice straw powder, 20-30 parts of coconut palm suede, ammonium molybdate
5-10 parts, 2-6 parts of boric acid, 3-8 parts of polyvinyl alcohol, 10-15 parts of hydroxypropyl-β-cyclodextrin, 10-15 parts of chitosan, growth hormone
1-5 parts, 10-20 parts of olive pomace；The compound Applying Ectomycorrhizal Fungi is colored acne Lasiosphaera fenzlii, delicious lactarius and suillusbovinus
Mixture, the dissolving phosphor and dissolving potassium bacterium are the mixture of bacillus megaterium and colloid bacillus cereus, and the compound fungus is length
The mixture of handle trichoderma and Streptomyces jingyangensis.
2. VA Mycorrhizal Fungi composite bacteria agent according to claim 1, it is characterised in that: raw material includes following components by weight:
6 parts of compound Applying Ectomycorrhizal Fungi, phosphorus decomposing, 4 parts of B. mucilaginocus, 4 parts of compound fungus, 25 parts of rice straw powder, 25 parts of coconut palm suede, 8 parts of ammonium molybdate,
4 parts of boric acid, 6 parts of polyvinyl alcohol, 12 parts of hydroxypropyl-β-cyclodextrin, 12 parts of chitosan, 3 parts of growth hormone, olive pomace 15
Part.
3. ectotrophic mycorrhiza composite bacteria agent according to claim 1, it is characterised in that: acne Lasiosphaera fenzlii colored in mass ratio: pine
Newborn mushroom: suillusbovinus=1:1:1.
4. Applying Ectomycorrhizal Fungi composite bacteria agent according to claim 3, it is characterised in that: gemma bar huge in mass ratio
Bacterium: colloid bacillus cereus=1:2.
5. Applying Ectomycorrhizal Fungi composite bacteria agent according to claim 4, it is characterised in that: long handle trichoderma in mass ratio: river rising in Ningxia and flowing into central Shaanxi
Positive streptomycete=2:1.
6. ectotrophic mycorrhiza composite bacteria agent according to claim 4, it is characterised in that: the growth hormone be methyl α-naphthyl acetate and
The mixture of indole-3-acetic acid.
7. ectotrophic mycorrhiza composite bacteria agent according to claim 5, it is characterised in that: methyl α-naphthyl acetate in mass ratio: indoles -3-
Acetic acid=2:1.
8. ectotrophic mycorrhiza composite bacteria agent according to claim 6, it is characterised in that: the chitosan has three-dimensional porous
Reticular structure.
9. ectotrophic mycorrhiza composite bacteria agent according to claim 7, it is characterised in that: be uniformly mixed to prepare above-mentioned raw materials
VA Mycorrhizal Fungi composite bacteria agent.
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| IT202000021661A1 (en) * | 2020-09-14 | 2022-03-14 | Cosvitec Scarl | "Use of pomace for the preparation of formulations for agriculture based on beneficial fungal microorganisms" |
| CN114504001A (en) * | 2022-01-11 | 2022-05-17 | 贵州大学 | Seed coating agent of masson pine ectomycorrhizal fungi, preparation and use method thereof |
| CN116622553A (en) * | 2023-04-07 | 2023-08-22 | 四川省食用菌研究所 | Lactarius deliciosus mycorrhiza growth promoting bacterium, and preparation method and application thereof |
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