CN105475363B - A kind of composite bacteria agent for promoting cucumber growth and its application - Google Patents
A kind of composite bacteria agent for promoting cucumber growth and its application Download PDFInfo
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- CN105475363B CN105475363B CN201510861441.5A CN201510861441A CN105475363B CN 105475363 B CN105475363 B CN 105475363B CN 201510861441 A CN201510861441 A CN 201510861441A CN 105475363 B CN105475363 B CN 105475363B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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Abstract
The invention discloses a kind of composite bacteria agent for promoting cucumber growth and its application.The microbial inoculum provided by the present invention for promoting cucumber growth, its active component are made up of more spore trichodermas (Trichoderma polysporum) ACCC31791 and Jia meter Li series bacillus (Paenibacillus jamilae) ACCC03134.The microbial inoculum has obvious growth promoting function to cucumber seedling, and environmentally friendly, and cost is low, pollution-free, environment can effectively be improved, Agro-ecological System balance is maintained, is advantageous to the popularization of pollution-free food and organic agriculture, powerful backing is provided to walk agricultural sustainable development road.
Description
Technical field
The present invention relates to a kind of composite bacteria agent for promoting cucumber growth and its application.
Background technology
Trichoderma (Trichoderma.spp) is to belong to partly to know a bacterium, hyphomycetales, trichoderma, is generally existing in soil
A kind of bacterium, it is common in root system of plant or edaphophyte residuum more.The bacterium colony initial stage of trichoderma is circle, white, after cultivating four days
Green spores are produced since center, final whole bacterium colony all becomes green.
Jia meter Li series bacillus (Paenibacillus jamilae) belongs to bacillus genus
(Paenibacillus) it is, a kind of Gram-negative bacteria, produces gemma, bacteria colony white or light yellow is raised, thick, surface
Smooth moistening, translucent, single bacterium is shaft-like.Jia meter Li series bacillus is one separated from maize straw manure at first
The series bacillus of the extracellular polysaccharide of strain, and for handling waste water (the Aguilera M et of olive factory
al.International Journal of Systematic and Evolutionary Microbiology,2001,51:
1687-1692).Jia meter Li series bacillus main functions have absorption heavy metal (Morillo P é rez J A et al.2008,
World Journal of Microbiology and Biotechnology, 24:2699-2704), olive factory is handled
Waste water (Morillo P é rez J A et al.World Journal of Microbiology and Biotechnology,
2007,23：1705-1710).
Cucumber is the important vegetables of Curcurbitaceae Cucumis, and because its yield is high, knot melon is early, quick, supply phase length, economy effect
Benefit is good, is generally cultivated in China north and south various regions and world wide, is one of most important vegetable crop.In order to promote cucumber
Growth, cucumber yield and quality are improved, should reasonably be applied fertilizer, growth of the application of organic fertilizers top dressing for cucumber is that extremely have
Profit.Beneficial microbe in microbial bacterial agent can improve soil granular structure, strengthen the physical property of soil and reduce soil
The loss of earth particle, under certain conditions, moreover it is possible to participate in humus and formed, so soil thing can be improved using microbial manure
Rationality shape, be advantageous to increase soil fertility, the growth of cucumber can not only be effectively facilitated, and for ecological environment of soil
It is beneficial.
The content of the invention
The technical problems to be solved by the invention are how to promote the growth of cucumber seedling.
In order to solve the above technical problems, present invention firstly provides the microbial inoculum for promoting cucumber growth.
The microbial inoculum provided by the present invention for promoting cucumber growth, its active component is by more spore trichodermas and Jia meter Li class gemma bars
Bacterium forms.
In above-mentioned microbial inoculum, more spore trichodermas are more spore trichodermas (Trichoderma polysporum) ACCC31791, institute
It is Jia meter Li series bacillus (Paenibacillus jamilae) ACCC03134 to state Jia meter Li series bacillus；More spores
The number of colony forming units ratio of trichoderma and the Jia meter Li series bacillus is 1:1.
In above-mentioned microbial inoculum, the content of the active component is more spore trichodermas and the Jia meter Li described in every gram of microbial inoculum
The content sum of this two plants of bacterial strains of series bacillus, can be 2 × 108-8×108Cfu/g, concretely 4 × 108cfu/g；It is described
The content of more spore trichodermas can be 2 × 108-8×108Cfu/g, concretely 2 × 108cfu/g；The Jia meter Li series bacillus
Content can be 2 × 108-8×108Cfu/g, concretely 2 × 108cfu/g。
Above-mentioned microbial inoculum may also include carrier, preferably pesticide field it is conventional and be biologically inert carrier.It is described
Carrier can be solid carrier or liquid-carrier.The solid carrier can be mineral material, vegetable material or high-molecular compound；Institute
It can be at least one of clay, talcum, kaolin, montmorillonite, white carbon, zeolite, silica and diatomite to state mineral material；It is described
Vegetable material can be at least one of corn flour, bean powder and starch；The high-molecular compound can be polyvinyl alcohol and/or gather
Glycol.The liquid-carrier can be organic solvent, vegetable oil, mineral oil or water；The organic solvent can be decane and/or 12
Alkane.In the microbial inoculum, the active component can be with the filter for the living cells, the zymotic fluid of living cells, cell culture being cultured
The form of liquid or cell and the mixture of filtrate is present.The living cells can be spore, mycelia or the bacterium containing spore and mycelia
The form of filament.The formulation of the composition can be a variety of formulations, such as liquor, emulsion, suspending agent, pulvis, granule, wettable
Pulvis or water dispersible granules.
As needed, surfactant (such as polysorbas20, Tween 80), adhesive, stably can be also added in the microbial inoculum
Agent (such as antioxidant), pH adjusting agent.
In order to solve the above technical problems, present invention also offers the preparation method of above-mentioned microbial inoculum.
The preparation method of above-mentioned microbial inoculum provided by the present invention, including by more spore Trichodermas and the Jia meter Li classes bud
Spore bacillus obtains the microbial inoculum as active component.
In the above method, more spore trichodermas are more spore trichodermas (Trichoderma polysporum) ACCC31791；Institute
It is Jia meter Li series bacillus (Paenibacillus jamilae) ACCC03134 to state Jia meter Li series bacillus；More spores
The number of colony forming units ratio of trichoderma and the Jia meter Li series bacillus is 1:1.
In the above method, the microbial inoculum specifically can be by more spore trichodermas and the Jia meter Li series bacillus microbial inoculum
It is mixed to get, the mixed proportion of more spore trichodermas and the Jia meter Li series bacillus microbial inoculum can be 1:1(w/w).
In the above method, more spore trichodermas are prepared by following methods：More spore trichoderma zymotic fluids and auxiliary material are carried out
It is mixed to get more spore trichodermas；The content of more spore trichodermas described in more spore trichoderma zymotic fluids can be 8 × 108-2×
109Cfu/mL, concretely 1 × 109cfu/mL；In more spore trichodermas, the content of more spore trichodermas is (2 × 108-
8×108) cfu/g, concretely 4 × 108cfu/g。
In the above method, the Jia meter Li series bacillus microbial inoculum is prepared by following methods：By Jia meter Li series bacillus
Zymotic fluid carries out being mixed to get the Jia meter Li series bacillus microbial inoculum with the auxiliary material；The Jia meter Li series bacillus fermentation
The content of Jia meter Li series bacillus described in liquid can be 1 × 109-2×109Cfu/mL, concretely 2 × 109cfu/mL；Institute
The content for stating Jia meter Li series bacillus described in Jia meter Li series bacillus microbial inoculums is (2 × 108-8×109) cfu/g, specifically
Can be 4 × 108cfu/g。
Above, the diatomite that the auxiliary material concretely sterilizes.
In order to solve the above technical problems, present invention also offers the microbial organic fertilizer for promoting cucumber growth.
It is provided by the present invention promote cucumber growth microbial organic fertilizer, by above-mentioned promotion cucumber growth microbial inoculum and have
Machine fertilizer composition.
In mentioned microorganism organic fertilizer, the organic fertilizer can be cow dung and mushroom residue according to 3:After 1 (w/w) mixed fermentation
Product.
The microbial inoculum of above-mentioned promotion cucumber growth provided by the present invention or the microbial organic fertilizer containing the microbial inoculum are promoting
The application entered in cucumber growth falls within the scope of protection of the invention.
In above-mentioned application, during the microbial inoculum is manured into soil, the dosage of the microbial inoculum applies 5 × 10 for every gram of soil5-2×
106Microbial inoculum described in cfu, concretely 2 × 106Microbial inoculum described in cfu；Specifically, the dosage of the microbial inoculum, which is every gram of soil, applies 1
×106More spore trichoderma (Trichoderma polysporum) ACCC31791 and 1 × 10 described in cfu6Jia meter Li classes bud described in cfu
Spore bacillus (Paenibacillus jamilae) ACCC03134.
In above-mentioned microbial inoculum, mentioned microorganism organic fertilizer and above-mentioned application, it is described promote cucumber growth be a1-a4 in 4 kinds,
3 kinds, 2 kinds or a kind：
A1, the growth for promoting cucumber seedling；
A2, the growth for promoting cucumber seedling root system；
A3, the growth for promoting cucumber seedling stem；
A4, the biomass for increasing cucumber seedling.
The biomass is the weight of cucumber seedling ground and/or under ground portion.
Further, the growth of the promotion cucumber seedling root system is presented as all or part in b1-b5：
B1, the total root long for increasing cucumber seedling；
B2, the root system total surface area for increasing cucumber seedling；
B3, the root system cumulative volume for increasing cucumber seedling；
Root system overall length in b4, increase cucumber seedling difference root diameter range；
B5, the density for increasing cucumber seedling root system；
The growth for promoting cucumber seedling stem is presented as the stem height and/or stem diameter of increase cucumber seedling.
It is demonstrated experimentally that it is more spore trichodermas (Trichoderma polysporum) ACCC31791 and Jia meter with active component
In series bacillus (Paenibacillus jamilae) ACCC03134 microbial inoculum and the microbial inoculum group that is jointly processed by of organic fertilizer it is yellow
Melon seedling, relative to organic fertilizer group, has obvious facilitation to cucumber seedling after 14d is grown.The ground of microbial inoculum group cucumber seedling
Part fresh weight is (8.4 ± 0.16) g, and aerial part fresh weight (4.6 ± 0.57) g than organic fertilizer group cucumber seedling increases by 82.61%；
The under ground portion fresh weight of microbial inoculum group cucumber seedling is (0.80 ± 0.010) g, than the under ground portion fresh weight (0.5 of organic fertilizer group cucumber seedling
± 0.010) g increases by 60.00%；The aerial part dry weight of microbial inoculum group cucumber seedling is (1.22 ± 0.107) g, more yellow than organic fertilizer group
Aerial part dry weight (0.28 ± 0.052) g increases by 335.71% of melon seedling；The under ground portion dry weight of microbial inoculum group cucumber seedling is
(0.112 ± 0.0086) g, under ground portion dry weight (0.069 ± 0.0052) g than organic fertilizer group cucumber seedling increase by 62.32%.Bacterium
Total root long of agent group cucumber seedling is (172.4 ± 23.83) m, and total root long (61.5 ± 2.79) cm than organic fertilizer group cucumber seedling increases
Long 180.33%；The root system total surface area of microbial inoculum group cucumber seedling is (18.9 ± 0.89) cm2, than the root system of organic fertilizer group cucumber seedling
Total surface area (7.3 ± 0.22) cm2Increase by 158.90%；The root system cumulative volume of microbial inoculum group cucumber seedling is (0.12 ± 0.003) cm3,
Than root system cumulative volume (0.07 ± 0.001) cm of organic fertilizer group cucumber seedling3Increase 71.43%；Microbial inoculum treatment group is in following diameter
In the range of cucumber seedling root length also all be considerably longer than organic fertilizer group：0mm-0.5mm, 0.5mm-4.5mm and>4.5mm.Bacterium
The root system of agent group cucumber seedling is denser than organic fertilizer group, and root area coverage is big, and capillary radical amount is more.The plant height of microbial inoculum group cucumber seedling
Increase 50.58% for (25.9 ± 2.39) cm, plant height (17.2 ± 1.06) cm than organic fertilizer group cucumber seedling；Microbial inoculum group cucumber seedling
Stem diameter be (0.64 ± 0.030) cm, than stem diameter (0.51 ± 0.011) cm thickenings 25.49% of organic fertilizer group cucumber seedling.
The microbial inoculum of the promotion cucumber growth of the present invention is environmentally friendly, and cost is low, pollution-free, can effectively improve environment, dimension
Agro-ecological System balance is held, is advantageous to the popularization of pollution-free food and organic agriculture, is provided to walk agricultural sustainable development road
Powerful backing.
Brief description of the drawings
Fig. 1 is the root system scanned photograph of each treatment group cucumber seedling.Wherein, A is microbial inoculum group, and B is organic fertilizer group, and C is to add rice
In microbial inoculum group, D is more spore microbial inoculum groups, and E is blank control group.
Embodiment
The present invention is further described in detail with reference to embodiment, the embodiment provided is only for explaining
The bright present invention, the scope being not intended to be limiting of the invention.
Experimental method in following embodiments, it is conventional method unless otherwise specified.
Material used, reagent etc., unless otherwise specified, are commercially obtained in following embodiments.
Jia meter Li series bacillus (Paenibacillus jamilae) ACCC03134 and more spores in following embodiments
Trichoderma (Trichoderma polysporum) ACCC31791 was concealed in Chinese microorganism strain before the applying date of the application
Preservation administration committee agricultural microorganism center, also known as Chinese agriculture Microbiological Culture Collection administrative center (Agricultural
Culture Collection of China, abbreviation ACCC, address：No.12 ,zhongguancun south street,Haidian District, Beijing, Chinese agriculture
Industry academy of sciences agricultural resource and agricultural regionalization research institute, postcode 100081), Jia meter Li series bacillus (Paenibacillus
Jamilae) ACCC03134 collection day is on December 20th, 2007, more spore trichodermas (Trichoderma polysporum)
ACCC31791 collection day it is August in 2007 1, from collecting, the public can entrust from Chinese Microbiological Culture Collection management
The agriculture microorganism center of member's meeting obtains the two bacterial strains.
Cucumber variety in following embodiments is Zhongnong No.16, is the production of Vegetable ＆ Flower Inst., Chinese Academy of Agriculture Science
Product.
Embodiment 1, the microbial inoculum of cucumber growth is promoted to test the growth promotion of cucumber
First, the microbial inoculum of cucumber growth is promoted to prepare
The preparation method for the culture medium used in the present embodiment is as follows：
PDA culture medium：The potato 200.0g of skin is removed, is cut into small pieces, adds water 1000mL to be filtered after boiling 30min, is received
Collect filtrate；Filtrate is complemented into 1000mL, and adds glucose 20.0g, agar 15.0g, is dispensed after fusing, 121 DEG C of sterilizings
15min。
The fermentation medium of more spore trichodermas：Sucrose 10g, corn flour 12g, Soybean Meal 5g, NH4NO32g、MgSO4·7H2O
0.3g、KH2PO42g, NaCl 2.5g, 7.0,121 DEG C of sterilizing 15min of distilled water 1L, pH.
Beef extract culture medium：Glucose 20g, peptone 15g, sodium chloride 5g, beef extract 0.5g, agar 20g, distilled water
7.0,121 DEG C of sterilizing 15min of 1L, pH.
The fermentation medium of series bacillus：Sucrose 15g, dregs of beans 10g, (NH4)2SO44g、K2HPO40.5g,MgSO4·
7H20.1g, pH6.5,121 DEG C of sterilizing 15min of O.
1st, the preparation of more spore trichoderma (Trichoderma polysporum) microbial inoculums
More spore trichodermas (Trichoderma polysporum) ACCC31791 is inoculated on PDA culture plates, 25 DEG C of trainings
72h is supported, then the spore on a culture plate is scraped to the 500mL of fermentation medium of the access equipped with the more spore trichodermas of 100mL
In triangular flask, at 25 DEG C, 48h is cultivated under the conditions of 180rpm；Then the fermentation of more spore trichodermas is inoculated into by 2% (v/v) inoculum concentration
In the 15L seeding tanks of culture medium, after cultivating 24h under the conditions of 25 DEG C, 220rpm, pH7.0, throughput 0.6vvm, then by 5%
(v/v) inoculum concentration is inoculated into the 100L of the fermentation medium equipped with the more spore trichodermas of 70L fermentation tank, at 25 DEG C, 220rpm,
3d is cultivated under the conditions of pH7.0, throughput 0.7vvm, obtains more spore trichoderma zymotic fluids, more spore trichodermas in more spore trichoderma zymotic fluids
(Trichoderma polysporum) ACCC31791 content is about 1 × 109cfu/mL.By more spore trichoderma zymotic fluids with going out
The diatomite of good bacterium is mixed, and obtains more spore trichodermas, more spore trichoderma (Trichoderma in more spore trichodermas
Polysporum) ACCC31791 content is 4 × 108cfu/g。
2nd, the preparation of Jia meter Li series bacillus (Paenibacillus jamilae) microbial inoculum
Jia meter Li series bacillus (Paenibacillus jamilae) ACCC 03134 is inoculated in beef extract culture and put down
On plate, 37 DEG C of culture 72h, the spore on a culture plate is then scraped into fermentation of the access equipped with 200mL series bacillus
In the 500mL triangular flasks of culture medium, at 37 DEG C, 72h is cultivated under the conditions of 220rpm；Then it is inoculated into dress by 2% (v/v) inoculum concentration
Have in the 15L seeding tanks of the fermentation medium of 8L series bacillus, at 37 DEG C, 220rpm, pH7.0, throughput 0.8vvm conditions
After lower culture 24h, then it is inoculated into by 5% (v/v) inoculum concentration the 100L of the fermentation medium equipped with 70L series bacillus hair
In fermentation tank, 4d is cultivated under the conditions of 37 DEG C, 220rpm, pH7.0, throughput 1.0vvm, obtains the fermentation of Jia meter Li series bacillus
Liquid, Jia meter Li series bacillus (Paenibacillus jamilae) ACCC03134 in Jia meter Li series bacillus zymotic fluids
Content is 2 × 109cfu/mL.Jia meter Li series bacillus zymotic fluid is mixed with the diatomite for bacterium of having gone out, obtains adding rice
In series bacillus microbial inoculum, Jia meter Li series bacillus (Paenibacillus in the Jia meter Li series bacillus microbial inoculums
Jamilae) ACCC03134 content is 4 × 108cfu/g。
3rd, the preparation of the microbial inoculum of cucumber growth is promoted
Jia meter Li series bacillus microbial inoculums prepared by more spore trichodermas and step 2 prepared by step 1 are according to 1:1(w/
W) mixed, be promoted the microbial inoculum of cucumber growth, its active component is by more spore trichoderma (Trichoderma
Polysporum) ACCC31791 and Jia meter Li series bacillus (Paenibacillus jamilae) ACCC 03134 is formed,
More spore trichoderma (Trichoderma polysporum) ACCC31791 and Jia meter Li class gemma in the microbial inoculum of the promotion cucumber growth
Bacillus (Paenibacillus jamilae) ACCC03134 number of colony forming units ratio is 1:1, the promotion cucumber growth
In microbial inoculum, more spore trichoderma (Trichoderma polysporum) ACCC31791 content is 2 × 108Cfu/g microbial inoculums, add rice
In series bacillus (Paenibacillus jamilae) ACCC03134 content be 2 × 108Cfu/g microbial inoculums；The work of microbial inoculum
Property composition content be more spore trichoderma (Trichoderma polysporum) ACCC31791 and Jia meter Li class buds in every gram of microbial inoculum
The content sum of this two plants of bacterial strains of spore bacillus (Paenibacillus jamilae) ACCC03134, it is 4 × 108Cfu/g microbial inoculums,
Used after packing.
2nd, the microbial inoculum of cucumber growth is promoted to the growth promoting function of cucumber
Soil is Beijing moisture soil, and using potted plant method, basin size is 18cm × 15cm.Organic fertilizer is cow dung and mushroom residue
According to 3：Product after the fermentation of 1 ratio (N, P, K content are respectively 5.56%).Experiment is divided into following 5 groups：Microbial inoculum group is to load per basin
1kg soil, the organic fertilizer of 10% soil weight is added, add the microbial inoculum that 5g promotes cucumber growth；More spore microbial inoculum groups are to be filled per basin
Enter 1kg soil, add the organic fertilizer of 10% soil weight, add the more spore microbial inoculums of 5g；Jia meter Li microbial inoculums group is to load 1kg's per basin
Soil, the organic fertilizer of 10% soil weight is added, add 5g Jia meter Li series bacillus microbial inoculums；Organic fertilizer group is that will load per basin
1kg soil, add the organic fertilizer of 10.5% soil weight；Blank control group (referred to as CK) is addition and microbial inoculum group gross weight phase
Same soil.
The cucumber seeds kind of prior vernalization (was designated as 0 moment) into circular matrix, (occurred a piece of true after waiting growth 7d
Leaf), seedling similar in selection growing way is moved on in the flowerpot of above-mentioned each group, is symmetrically distributed in basin, per 2, basin, every group of basin of repetition 6,
(30 DEG C or so of daytime, 20 DEG C or so of night) is planted in greenhouse.
It is fresh to the weight of cucumber seedling, including aerial part/under ground portion of cucumber seedling after cucumber transplantation of seedlings grows 14d
Weight and aerial part/under ground portion dry weight carry out statistical analysis, as a result as shown in table 1：The weight of microbial inoculum group cucumber seedling is significantly high
In other each groups, the aerial part fresh weight of microbial inoculum group cucumber seedling is (8.4 ± 0.16) g, than the overground part of organic fertilizer group cucumber seedling
Divide fresh weight (4.6 ± 0.57) g increases by 82.61%, than aerial part fresh weight (6.0 ± 0.21) g of Jia meter Li microbial inoculum group cucumber seedlings
Increase by 40.00%, aerial part fresh weight (8.3 ± 0.64) g than more spore microbial inoculum group cucumber seedlings increase by 1.2%, compare blank control
Aerial part fresh weight (2.9 ± 0.51) g increases by 189.66% of group cucumber seedling；The under ground portion fresh weight of microbial inoculum group cucumber seedling is
(0.80 ± 0.010) g, under ground portion fresh weight (0.5 ± 0.010) g than organic fertilizer group cucumber seedling increases by 60.00%, than adding rice
In microbial inoculum group cucumber seedling under ground portion fresh weight (0.53 ± 0.058) g increase by 50.94%, than the ground of more spore microbial inoculum group cucumber seedlings
Lower part fresh weight (0.67 ± 0.028) g increase by 19.40%, than blank control group cucumber seedling under ground portion fresh weight (0.43 ±
0.058) g increases by 86.05%；The aerial part dry weight of microbial inoculum group cucumber seedling is (1.22 ± 0.107) g, than organic fertilizer group cucumber
Aerial part dry weight (0.28 ± 0.052) g increases by 335.71% of seedling, than the aerial part dry weight of Jia meter Li microbial inoculum group cucumber seedlings
(0.58 ± 0.019) g increases by 110.34%, than aerial part dry weight (0.79 ± 0.042) g increases of more spore microbial inoculum group cucumber seedlings
54.43%, aerial part dry weight (0.22 ± 0.087) g than blank control group cucumber seedling increases by 454.55%；Microbial inoculum group cucumber
The under ground portion dry weight of seedling is (0.112 ± 0.0086) g, than organic fertilizer group cucumber seedling under ground portion dry weight (0.069 ±
0.0052) g increases by 62.32%, than under ground portion dry weight (0.07 ± 0.0046) g increases of Jia meter Li microbial inoculum group cucumber seedlings
60.00%, lower part dry weight (0.102 ± 0.0068) g than more spore microbial inoculum group cucumber seedling ground increases by 9.80%, compares blank control
Under ground portion dry weight (0.042 ± 0.0021) g increases by 166.67% of group cucumber seedling.
The weight (g) of table 1, different disposal group cucumber seedling
Note:* represents significant difference, P<0.05.
The development degree of root system determines plant growth amount, and root system is more flourishing, and plant growth is better.Treat that cucumber seedling grows 14d
Afterwards, analysis is compared to the root growth situation of each treatment group, as a result as shown in table 2：Total root long of microbial inoculum group cucumber seedling, root
It is surface area and root system volume is all remarkably higher than other each groups, the average root diameter of each treatment group cucumber seedling is not significantly different.
Total root long of microbial inoculum group cucumber seedling is (172.4 ± 23.83) cm, than total root long (61.5 ± 2.79) cm of organic fertilizer group cucumber seedling
Increase by 180.33%, total root long (54.7 ± 5.91) cm than Jia meter Li microbial inoculum group cucumber seedlings increases by 215.17%%, than more spores
Total root long (117.1 ± 13.51) cm of microbial inoculum group cucumber seedling increases by 47.22%, than total root long of blank control group cucumber seedling
(28.3 ± 1.12) cm increases by 509.19%；The root system total surface area of microbial inoculum group cucumber seedling is (18.9 ± 0.89) cm2, than organic
Root system total surface area (7.3 ± 0.22) cm of fertilizer group cucumber seedling2Increase by 158.90%, than the root system of Jia meter Li microbial inoculum group cucumber seedlings
Total surface area (6.6 ± 0.47) cm2Increase by 186.36%, than more spore microbial inoculum group cucumber seedlings root system total surface area (12.0 ±
1.66)cm2Increase by 57.50%, than root system total surface area (3.3 ± 0.33) cm of blank control group cucumber seedling2Increase
472.73%；The root system cumulative volume of microbial inoculum group cucumber seedling is (0.12 ± 0.003) cm3, the root system than organic fertilizer group cucumber seedling is total
Volume (0.07 ± 0.001) cm3Increase 71.43%, than the root system cumulative volume (0.06 ± 0.005) of Jia meter Li microbial inoculum group cucumber seedlings
cm3Increase 100%, root system cumulative volume (0.12 ± 0.008) cm with more spore microbial inoculum group cucumber seedlings3Equally, it is more yellow than blank control group
Root system cumulative volume (0.03 ± 0.001) cm of melon seedling3Increase 300.00%.Cucumber of the microbial inoculum treatment group in following diameter range
The root length of seedling is also all significantly higher than other each treatment groups：0mm-0.5mm, 0.5mm-4.5mm and>4.5mm (table 3).In Fig. 1
As a result show, the root system of microbial inoculum group cucumber seedling is denser than other each treatment groups, and root area coverage is big, and capillary radical amount is more.
The root growth situation of table 2, different disposal group
Note:* represents significant difference, P<0.05.
Root length (cm) in the range of table 3, different-diameter
Note:* represents significant difference, P<0.05.
After table 4 is cucumber seedling growth 14d, the aerial part statistical result of each treatment group cucumber seedling, microbial inoculum group cucumber seedling
Plant height be significantly higher than other each treatment groups, footpath, which is enclosed, is significantly coarser than other each treatment groups, the number of blade then no significant difference.Microbial inoculum group
The plant height of cucumber seedling is (25.9 ± 2.39) cm, and plant height (17.2 ± 1.06) cm than organic fertilizer group cucumber seedling increases 50.58%,
Plant height (16.4 ± 1.19) cm than Jia meter Li microbial inoculum group cucumber seedlings increases 57.93%, than the plant height of more spore microbial inoculum group cucumber seedlings
(22.0 ± 1.48) cm increases 17.73%, and plant height (11.9 ± 1.10) cm than blank control group cucumber seedling increases 117.65%；
The stem diameter of microbial inoculum group cucumber seedling is (0.64 ± 0.030) cm, than stem diameter (0.51 ± 0.011) cm of organic fertilizer group cucumber seedling
Thickening 25.49%, than stem diameter (0.47 ± 0.031) cm thickenings 36.17% of Jia meter Li microbial inoculum group cucumber seedlings, with more spore microbial inoculums
Stem diameter (0.65 ± 0.087) cm of group cucumber seedling is consistent, than stem diameter (0.36 ± 0.040) cm of blank control group cucumber seedling
Thickening 77.78%.
Table 4, different disposal group cucumber seedling aerial growth situation
Note:* represents significant difference, P<0.05.
Claims (11)
1. microbial inoculum or application of the microbial organic fertilizer in cucumber growth is promoted containing the microbial inoculum, the activity of the microbial inoculum into
Divide and be made up of more spore trichodermas and Jia meter Li series bacillus；More spore trichodermas are more spore trichoderma (Trichoderma
Polysporum) ACCC31791, the Jia meter Li series bacillus are Jia meter Li series bacillus (Paenibacillus
Jamilae the number of colony forming units ratio of) ACCC03134, more spore trichodermas and the Jia meter Li series bacillus is 1:
1。
2. application according to claim 1, it is characterised in that：During the microbial inoculum is manured into soil, the dosage of the microbial inoculum is
Every gram of soil applies (5 × 105-2×106) microbial inoculum described in cfu.
3. application according to claim 1 or 2, it is characterised in that：It is described to promote cucumber growth as 4 kinds in a1-a4,3
Plant, 2 kinds or a kind：
A1, the growth for promoting cucumber seedling；
A2, the growth for promoting cucumber seedling root system；
A3, the growth for promoting cucumber seedling stem；
A4, the biomass for increasing cucumber seedling.
4. application according to claim 3, it is characterised in that：The growth for promoting cucumber seedling root system is presented as b1-b5
In all or part：
B1, the total root long for increasing cucumber seedling；
B2, the root system total surface area for increasing cucumber seedling；
B3, the root system cumulative volume for increasing cucumber seedling；
Root system overall length in b4, increase cucumber seedling difference root diameter range；
B5, the density for increasing cucumber seedling root system；
The growth for promoting cucumber seedling stem is presented as the stem height and/or stem diameter of increase cucumber seedling.
5. promote the microbial inoculum of cucumber growth, it is characterised in that：The active component of the microbial inoculum more spore wood described in claim 1
Jia meter Li series bacillus composition described in mould and claim 1.
6. microbial inoculum according to claim 5, it is characterised in that：It is described promote cucumber growth be a1-a4 in 4 kinds, 3 kinds, 2
Kind or a kind：
A1, the growth for promoting cucumber seedling；
A2, the growth for promoting cucumber seedling root system；
A3, the growth for promoting cucumber seedling stem；
A4, the biomass for increasing cucumber seedling.
7. microbial inoculum according to claim 6, it is characterised in that：The growth for promoting cucumber seedling root system is presented as b1-b5
In all or part：
B1, the total root long for increasing cucumber seedling；
B2, the root system total surface area for increasing cucumber seedling；
B3, the root system cumulative volume for increasing cucumber seedling；
Root system overall length in b4, increase cucumber seedling difference root diameter range；
B5, the density for increasing cucumber seedling root system；
The growth for promoting cucumber seedling stem is presented as the stem height and/or stem diameter of increase cucumber seedling.
8. the preparation method of any microbial inoculum in claim 5-7, including by more spore trichodermas and right described in claim 1
It is required that Jia meter Li series bacillus described in 1 obtains the microbial inoculum as active component.
9. promoting the microbial organic fertilizer of cucumber growth, it is made up of any described microbial inoculum and organic fertilizer in claim 5-7.
10. microbial organic fertilizer according to claim 9, it is characterised in that：The promotion cucumber growth is in a1-a4
4 kinds, 3 kinds, 2 kinds or a kind：
A1, the growth for promoting cucumber seedling；
A2, the growth for promoting cucumber seedling root system；
A3, the growth for promoting cucumber seedling stem；
A4, the biomass for increasing cucumber seedling.
11. microbial organic fertilizer according to claim 10, it is characterised in that：
The growth of the promotion cucumber seedling root system is presented as all or part in b1-b5：
B1, the total root long for increasing cucumber seedling；
B2, the root system total surface area for increasing cucumber seedling；
B3, the root system cumulative volume for increasing cucumber seedling；
Root system overall length in b4, increase cucumber seedling difference root diameter range；
B5, the density for increasing cucumber seedling root system；
The growth for promoting cucumber seedling stem is presented as the stem height and/or stem diameter of increase cucumber seedling.
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