CN101805216B - Method for locally culturing and producing arbuscular mycorrhiza fungus biological agent fertilizer in farmlands - Google Patents
Method for locally culturing and producing arbuscular mycorrhiza fungus biological agent fertilizer in farmlands Download PDFInfo
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- CN101805216B CN101805216B CN2010101345951A CN201010134595A CN101805216B CN 101805216 B CN101805216 B CN 101805216B CN 2010101345951 A CN2010101345951 A CN 2010101345951A CN 201010134595 A CN201010134595 A CN 201010134595A CN 101805216 B CN101805216 B CN 101805216B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
Abstract
The invention provides a method for locally culturing and producing arbuscular mycorrhiza fungus biological agent fertilizer in farmlands. The method is characterized by comprising the following steps of: 1, preparing a farmland culturing pond and a culture medium; 2, transplanting and culturing arbuscular mycorrhiza fungi; 3, absorbing and converting biological fertilizer; and 4, collecting biological fertilizer. Compared with the prior art, the technique adopted in the method has the advantages that: 1, the raw materials are readily available, the culture of the raw materials is easy, and the cost is low; 2, the culture technique and the production technique are conveniently popularized in rural areas; and 3, the production technique is green and environment friendly, which facilitates the development of modern agriculture. In addition, by using the fertilizer of the invention, the used amount of the quick-acting fertilizer is reduced and the used amount of partial fertilizer is reduced, so that the pollution to soil, water resources and air is lowered, and the fertilizer is particularly suitable to be used for planting seedlings of farm crops and medicinal plants. Tests show that the yield of the farm crops cultured by using the fertilizer of the method is improved by over 50 percent compared with that of the farm crops cultured by using the common fertilizer.
Description
Technical field
The invention belongs to the production technical field of bio-feritlizer, be specifically related to a kind of farmland method of cultivating and producing bush mycorrhizal fungi bacteria agent fertilizer on the spot.
Background technology
(arbuscular mycorrhizal fungi is ancient and symbiote widely has symbiotic relationship with the plant of land more than 80% AMF) to bush mycorrhizal fungi.In this cogeneration system, plant can flow to the endophyte silk (generally being hexose) of AM fungi through photosynthesis synthetic carbohydrate, and the endophyte silk flows to external mycelia to the synthetic lipid of hexose, for the growth and breeding of this bacterium again.The AM fungi stretches mycelia in the soil, thereby has enlarged the absorption surface of plant, can improve the absorption to the soil nutrient material, and like materials such as P, N, S, Zn and water, enhancement of plant is to the resistibility and the drought-resistant ability of disease.It is narrow spectrum that but the AM fungi is symbiosis, must be under the condition that has the roots of plants host to exist could growth and breeding, thereby accomplish its life working cycle, so the production of its microbial inoculum difficulty then, the application in actual production is restricted always.
The AM fungi is a most widespread mycorhiza type in the world, and its parasitic scope is much more extensive than ectomycorrhiza.Current biological technology of mycorrhiza research direction at first is a large amount of breeding problems of mycorhiza inoculum, particularly AM fungi.China possesses AM fungal inoculant production technology basically, yet, owing to lack the input of Infrastructure and equipment, add transportation and the use cost height applied in the farmland, do not carry out large-scale industrialized production so far.
And AM fungal organism active fertilizer technology is cultivated in the development farmland on the spot, goes for the production application of green and organic farm products, reduces use cost greatly, and is easy to utilize.Give birth to the AM fungi in utilizing and effectively to absorb organic farm manure (animal excrement) and other different types of nitrogenous source (monosodium glutamate waste liquid and protein hydrolyte), like NO
3 -, NH
4 +, amino acid and soluble protein, the absorption that also can improve other fertilizer (phosphorus P).The active microbial inoculum fertilizer of AM fungal organism has the effect of slow-release nitrogen fertilizer through absorbing the utilising efficiency that inorganic and organonitrogen can improve these common nitrogenous fertilizer, reduces the nitrate content in the river, lake.
In addition, current bio-feritlizer mainly concentrates on bacterium, like root nodule bacterium, and vinelandii, silicate bacteria, phosphorus decomposing and potassium solubilizing bacteria also do not have symbiosis Mycophyta bio-feritlizer.
Summary of the invention
To the problem that prior art exists, the objective of the invention is to design provides a kind of farmland technical scheme of cultivating and producing bush mycorrhizal fungi bacteria agent fertilizer on the spot, and this method is simple, and is with low cost, and the utilization and extention in the rural area is convenient in environmental protection.
Described farmland is the method for cultivating and producing bush mycorrhizal fungi bacteria agent fertilizer on the spot, it is characterized in that may further comprise the steps:
1) making of farmland cultivation pool and culture medium
In the farmland, surround cultivation pool with polyethylene plastic film and timber, in cultivation pool, add by showing tremendous enthusiasm gather 5-15 part vermiculite 40-60 part, the culture medium that humic properties soil 20-30 part mixes then;
2) transplant bush mycorrhizal fungi symbiote and cultivation thereof
With the symbiote of cultured host plant and bush mycorrhizal fungi, transplant to cultivation pool and cultivate, between during cultivation; Each week is watered nutritive medium once in culture medium; In every strain symbiote, pour into simultaneously root again and leach stoste, compound amino-acid nutrition liquid and ammonium humate solution
Described nutrient solution prescription is: KNO
3300-500mg/L, Ca (NO
3)
2520-720mg/L, MgSO
4160-360mg/L, H
3BO
33-6mg/L, MnCl
2.4H
2O 1-5mg/L, ZnSO
4.7H
2O 1-5mg/L, CuSO
4.5H
2O 0.05-1mg/L, H
2MoO
4.H
2O 0.05-1mg/L,
Described leaching stoste is: the liquid that the Radix Dauci Sativae root tissue that cultivation Ri T-DNA transforms obtains,
Described compound amino-acid nutrition liquid is: the mixing solutions of 17 kinds of standard amino acids;
3) bio-feritlizer absorbs and transforms
The symbiote of host plant and bush mycorrhizal fungi is cultivated 6-8 after week in cultivation pool; With 300-500 order nylon wire cultivation pool is being divided into two Room from host plant 10-15cm place; In not having a Room cultivation pool of symbiote; Add animal excrement, monosodium glutamate waste liquid or protein hydrolyte are so that pass the absorption conversion that the bush mycorrhizal fungi mycelia of nylon wire carries out bio-feritlizer;
4) collection of bio-feritlizer
After the absorption of bio-feritlizer transforms 4-8 week, remove leaf and the stem of host plant, collect root and the bush mycorrhizal fungi mycelia of host plant, and and cultivation pool in the culture medium stirring, promptly obtain bush mycorrhizal fungi bacteria agent fertilizer.
Described farmland is the method for cultivating and producing bush mycorrhizal fungi bacteria agent fertilizer on the spot, it is characterized in that culture medium contains showing tremendous enthusiasm gather 10-15 part in the described step 1), vermiculite 50-60 part, humic properties soil 25-30 part.
Described farmland is the method for cultivating and producing bush mycorrhizal fungi bacteria agent fertilizer on the spot; It is characterized in that described step 2) described in the host plant be onion or paspalum notatum, described bush mycorrhizal fungi is mould, the Glomus mosseae of sacculus in the root, children's cover sacculus is mould or one or more mixing thalline of native country bush mycorrhizal fungi.
Described farmland is the method for cultivating and producing bush mycorrhizal fungi bacteria agent fertilizer on the spot; It is characterized in that described step 2) described in host plant and the symbiote of bush mycorrhizal fungi reach 50-90% for host's root infection rate, spore count reaches the symbiote of 10-20/g.
Described farmland is the method for cultivating and producing bush mycorrhizal fungi bacteria agent fertilizer on the spot; It is characterized in that described step 2) in each week water the 15-25ml nutritive medium once; In every strain symbiote, add root simultaneously again and leach stoste 10-25ml; Compound amino-acid nutrition liquid 15-25ml, concentration is the ammonium humate solution 2-5ml of 1-3%
Described nutritive medium contains: KNO
3350-450mg/L, Ca (NO
3)
2550-600mg/L, MgSO
4200-300mg/L, H
3BO
34-5mg/L, MnCl
2.4H
2O 2-4mg/L, ZnSO
4.7H
2O 2-4mg/L, CuSO
4.5H
2O 0.1-0.5mg/L, H
2MoO
4.H
2O 0.1-0.5mg/L.
Described farmland is the method for cultivating and producing bush mycorrhizal fungi bacteria agent fertilizer on the spot, it is characterized in that nylon wire is 400 orders in the described step 3).
Described farmland is the method for cultivating and producing bush mycorrhizal fungi bacteria agent fertilizer on the spot; When it is characterized in that described step 3) mycelia carries out the absorption conversion of bio-feritlizer; Blade through cutting off part host plant or be 0.5% amino acid foliage fertilizer to host's plant leaf spray concentration, and water and execute the choline dichloride diluent.
Described farmland is the method for cultivating and producing bush mycorrhizal fungi bacteria agent fertilizer on the spot, adds concentration in the bush mycorrhizal fungi bacteria agent fertilizer that it is characterized in that obtaining in the described step 4) and is 1.0% glucose solution and leach stoste with root and be prepared into the farmland with cultivating fertilizer.
Described farmland is the method for cultivating and producing bush mycorrhizal fungi bacteria agent fertilizer on the spot, it is characterized in that described bush mycorrhizal fungi bacteria agent fertilizer, glucose solution and root leaching stoste three's weight ratio is 100: 0.5-5: 0.05-0.5.
Give birth to the AM hypha,hyphae in the present invention utilizes and absorb the ability of inorganic, organonitrogen and other nutritive substance; And mycelia stores and carry N to give the mechanism of plant; Use special culture medium and plant host, cultivate the symbiote (mycelia and spore) of good this bacterium, cultivate this symbiote in large quantity at cultivated land by peasant oneself again; Simultaneously can apply fertilizer and chemical nitrogen fertilizer, even can use waste gourmet powder fermenting liquor (to contain high density (NH
4)
2SO
4And multiple amino acids), for after thalline fully absorbs, N mainly with l-arginine (Arg) stores in thalline, be made into the root that bacteria agent fertilizer is seeded in farm crop again, come increasing both production and income.
It is main composite microbiological fertilizer or biological nitrogen fertilizer that this bacteria agent fertilizer is different from vinelandii; But with the propagulum (spore of AM fungi; Mycelia) is microbial inoculum; Be stored in endobacillary nitrogen as biological nitrogen (mainly be l-arginine) from the culture medium absorption is inorganic with organonitrogen with it, flow to plant symbiotic to endobacillary biological nitrogen again, accomplish the process that absorbs of nitrogen with it.This biological nitrogen fertilizer microbial inoculum is strong not as root nodule bacterium to host specificity, and most plant all can be infected by the AM fungi.Except having the effect of nitrogenous fertilizer, also have the activating soil nutrient, improve the crop mineral matter nutritional; Improve plant drought and resistance against diseases; Promote growth and development of plants, increase output and improve biological effect such as quality.
Compared with prior art there is following beneficial effect in the above-mentioned farmland method of cultivating and producing bush mycorrhizal fungi bacteria agent fertilizer on the spot: 1) draw materials easily, cultivate simple, with low cost; 2) culture technique and production technology are convenient to apply in the rural area; 3) production technology environmental protection helps developing modern agriculture.
And the fertilizer that the present invention obtains can reduce the consumption of quick-acting chemical fertilizer, reduces the amount of application of part agricultural chemicals, thereby can be particularly useful for the cultivation of farm crop seedling and medicinal plant to alleviate soil, water resources and atmospheric pollution.Test shows that the farm crop of using fertilizer of the present invention reach more than 50% than the increasing crop yield of common cultivation.
Embodiment
Below further specify the present invention through embodiment.
Embodiment 1
1) making of farmland cultivation pool and culture medium
In the farmland; Surround cultivation pool with polyethylene plastic film and timber, the spacing of timber is 60cm * 60cm, and around the cultivation pool and bottom surrounds with plastic film; The volume of cultivation pool is 60cm * 60cm * 20cm; Volume according to cultivation pool adds in cultivation pool by 15 parts of showing tremendous enthusiasm gathers then, 50 parts of vermiculites, 25 parts of culture mediumes that mix of humic properties soil; Showing tremendous enthusiasm gather is that farmland earth is pulverized behind rice straw burning and formed, and vermiculite is bought from market, and humic properties soil is piled up by earth and the residual branch of leaf and obtained after rotting;
2) transplant arbuscular mycorrhiza symbiote and cultivation thereof
The symbiote of host plant and bush mycorrhizal fungi: make culture vessel, get the stainless steel keg, size is 6.0 * 1.1 * 22.5cm
3(back cut diameter * lower port diameter * height), the bottom is porose, and the hole is blocked with hospital gauze, and the dark and hollow out of keg helps fungi mycorhiza root system like this and grow downwards, and the bottom can ponding.Every barreled 160g sand; Selecting the over-ground part height is 4-7cm, and the long growing way that two tender leafs are arranged preferably host's paspalum notatum or onion seedling replanting to keg, 2 strain/buckets; With the about 10-30 of a bush mycorrhizal fungi spore inoculating to the root of host's seedling; Bush mycorrhizal fungi can be the mould Glomusintraradices of sacculus in the root, Glomus mosseae Glomus mosseae, the mixing thalline of one or more of children cover mould Glomus etunicatum of sacculus or native country bush mycorrhizal fungi.After the transplanting, add 2ml/ bucket Hoagland nutritive medium weekly, watered 1 time water in per two days.
Cultivating 6-8 after week, host's root infection rate reaches more than 50%, when spore count reaches 10/g, cultivates in the cultivation pool of transplanting symbiote to step 1), and each cultivation pool is transplanted the cultured symbiote of 5 strains, and the spacing of symbiote plant is 20cm * 20cm.Between during cultivation; Each week is watered the 20ml nutritive medium once, in culture medium around every strain symbiote, pours into root simultaneously again and leaches stoste 15ml, compound amino-acid nutrition liquid 20ml; With concentration be 3% the every strain of ammonium humate solution 4ml/, these nutritional factor help breeding and the expansion of mycelia.
Described nutrient solution prescription is: KNO
3400mg/L, Ca (NO
3)
2620mg/L, MgSO
4260mg/L, H
3BO
34.96mg/L, MnCl
2.4H
2O 2.8mg/L, ZnSO
4.7H
2O 2.22mg/L, CuSO
4.5H
2O0.18mg/L, H
2MoO
4.H
2O 0.19mg/L does not add phosphoric acid salt.
Described making method that leaches stoste: the Radix Dauci Sativae root that Ri T-DNA transforms is cultivated in the petridish that contains the M solid medium; Carefully root is transplanted in the liquid M substratum that contains sucrose 24 ℃ of 4 weeks of growth; Collect at last and obtain root and leach stoste, be stored in-20 ℃ subsequent use.
Described compound amino-acid nutrition liquid (concentration is about 0.1mmol) is: contain 17 kinds of standard amino acid mixing solutionss.Aspartic acid, Threonine, Serine, L-glutamic acid, glycocoll, L-Ala, Gelucystine, Xie Ansuan, methionine(Met), Isoleucine, leucine, tyrosine, phenylalanine(Phe), lysine hydrochloride, Histidine, l-arginine and proline(Pro).
3) bio-feritlizer absorbs and transforms
The symbiote of host plant and bush mycorrhizal fungi cultivated for 6 weeks in cultivation pool after; With 400 order nylon wires cultivation pool is being divided into two Room from host plant 15cm place; In not having a Room cultivation pool of symbiote, add animal excrement, monosodium glutamate waste liquid or protein hydrolyte; Bush mycorrhizal fungi passes nylon wire and reaches in another chamber cultivation pool, and the absorption of carrying out bio-feritlizer transforms;
4) storage of biological nitrogen
When the absorption that mycelia carries out bio-feritlizer transforms, the blade through cutting off part host plant or be 0.5% amino acid foliage fertilizer, consumption 20ml/ strain to host's plant leaf spray concentration; And water by every strain 30-50 milliliter with the choline dichloride diluent of 500 mg/litre and to execute;
5) collection of bio-feritlizer
After the absorption of bio-feritlizer transformed for 6 week, remove leaf and the stem of host plant, collect root and the bush mycorrhizal fungi mycelia of host plant, and with cultivation pool in the culture medium stirring, promptly obtain bush mycorrhizal fungi bacteria agent fertilizer; With MPN method detection of biological fertilizer bacterial agent product, contain biological nitrogen in the about 2.5mg/100mg of l-arginine (mycorhiza propagulum), the dried microbial inoculum fertilizer of active sporogenesis body 80-100/restrain.
6) bio-feritlizer is used
The bush mycorrhizal fungi bacteria agent fertilizer that obtains is the weight ratio 100: 1: 0.5 that 1% glucose solution and root leach the stoste three by bush mycorrhizal fungi bacteria agent fertilizer, concentration, makes the farmland with cultivation fertilizer, is used for the farmland and cultivates seedling.
Embodiment 2
Adopt 5 parts of showing tremendous enthusiasm gathers in the step 1), 40 parts of vermiculites, 20 parts in humic properties soil; Step 2) adopt host's root infection rate to reach more than 80% in; Spore count reaches the symbiote of 20/g, and between during cultivation, each week is watered the 15ml nutritive medium once; In culture medium around every strain symbiote, pour into root simultaneously again and leach stoste 10ml; Compound amino-acid nutrition liquid 15ml and concentration are 2% the every strain of ammonium humate solution 5ml/, and the nutrient solution prescription of employing is: KNO
3500mg/L, Ca (NO
3)
2720mg/L, MgSO
4360mg/L, H
3BO
36mg/L, MnCl
2.4H
2O 5mg/L, ZnSO
4.7H
2O 5mg/L, CuSO
4.5H
2O 1mg/L, H
2MoO
4.H
2O 1mg/L does not add phosphoric acid salt; The symbiote of host plant and bush mycorrhizal fungi is cultivated 6-8 after week in the step 3) in cultivation pool, with 500 order nylon wires cultivation pool is being divided into two Room from host plant 12cm place; Bio-feritlizer absorbs and transformed for 4 weeks in the step 4); Other steps are identical with embodiment 1; Obtain having the bush mycorrhizal fungi bacteria agent fertilizer of same effect at last with embodiment 1; The weight ratio that leaches the stoste three by bush mycorrhizal fungi bacteria agent fertilizer, glucose solution and root then is to carry out proportioning at 100: 0.5: 0.05; Process the farmland and be used for arviculture, also can obtain at last the technique effect identical with embodiment 1 with cultivation fertilizer.
Embodiment 3
Adopt 10 parts of showing tremendous enthusiasm gathers in the step 1), 60 parts of vermiculites, 25 parts in humic properties soil; Step 2) adopt host's root infection rate to reach more than 70% in; Spore count reaches the symbiote of 15/g, and between during cultivation, each week is watered the 25ml nutritive medium once; In culture medium around every strain symbiote, pour into root simultaneously again and leach stoste 25ml; Compound amino-acid nutrition liquid 25ml and concentration are 3% the every strain of ammonium humate solution 2ml/, and the nutrient solution prescription of employing is: KNO
3300mg/L, Ca (NO
3)
2520mg/L, MgSO
4160mg/L, H
3BO
33mg/L, MnCl
2.4H
2O 1mg/L, ZnSO
4.7H
2O 1mg/L, CuSO
4.5H
2O 0.05mg/L, H
2MoO
4.H
2O 0.05mg/L does not add phosphoric acid salt; After the symbiote of host plant and bush mycorrhizal fungi cultivated for 7 weeks in the step 3) in cultivation pool, with 400 order nylon wires cultivation pool is being divided into two Room from host plant 15cm place; Bio-feritlizer absorbs and transformed for 6 weeks in the step 4); Other steps are identical with embodiment 1; Obtain having the bush mycorrhizal fungi bacteria agent fertilizer of same effect at last with embodiment 1; The weight ratio that leaches the stoste three by bush mycorrhizal fungi bacteria agent fertilizer, glucose solution and root then is to carry out proportioning at 100: 2: 0.3; Process the farmland and be used for arviculture, also can obtain at last the technique effect identical with embodiment 1 with cultivation fertilizer.
Claims (9)
1. the farmland method of cultivating and producing bush mycorrhizal fungi bacteria agent fertilizer on the spot is characterized in that may further comprise the steps:
1) making of farmland cultivation pool and culture medium
In the farmland; Surround cultivation pool with polyethylene plastic film and timber, in cultivation pool, add then by showing tremendous enthusiasm gather 5-15 part, vermiculite 40-60 part; The culture medium that humic properties soil 20-30 part mixes, described showing tremendous enthusiasm gather are that farmland earth is pulverized behind rice straw burning and formed;
2) transplant bush mycorrhizal fungi symbiote and cultivation thereof
With the symbiote of cultured host plant and bush mycorrhizal fungi, transplant to cultivation pool and cultivate, between during cultivation; Each week is watered nutritive medium once in culture medium; In every strain symbiote, pour into simultaneously root again and leach stoste, compound amino-acid nutrition liquid and ammonium humate solution
Described nutrient solution prescription is: KNO
3300-500mg/L, Ca (NO
3)
2520-720mg/L, MgSO
4160-360mg/L, H
3BO
33-6mg/L, MnCl
2.4H
2O 1-5mg/L, ZnSO
4.7H
2O 1-5mg/L, CuSO
4.5H
2O 0.05-1mg/L, H
2MoO
4.H
2O 0.05-1mg/L,
Described leaching stoste is: the liquid that the Radix Dauci Sativae root tissue that cultivation Ri T-DNA transforms obtains,
Described compound amino-acid nutrition liquid is: the mixing solutions of 17 kinds of standard amino acids;
3) bio-feritlizer absorbs and transforms
The symbiote of host plant and bush mycorrhizal fungi is cultivated 6-8 after week in cultivation pool; With 300-500 order nylon wire cultivation pool is being divided into two Room from host plant 10-15cm place; In not having a Room cultivation pool of symbiote; Add animal excrement, monosodium glutamate waste liquid or protein hydrolyte are so that pass the absorption conversion that the bush mycorrhizal fungi mycelia of nylon wire carries out bio-feritlizer;
4) collection of bio-feritlizer
After the absorption of bio-feritlizer transforms 4-8 week, remove leaf and the stem of host plant, collect root and the bush mycorrhizal fungi mycelia of host plant, and and cultivation pool in the culture medium stirring, promptly obtain bush mycorrhizal fungi bacteria agent fertilizer.
2. farmland as claimed in claim 1 is the method for cultivating and producing bush mycorrhizal fungi bacteria agent fertilizer on the spot, it is characterized in that culture medium contains showing tremendous enthusiasm gather 10-15 part in the described step 1), vermiculite 50-60 part, humic properties soil 25-30 part.
3. farmland as claimed in claim 1 is the method for cultivating and producing bush mycorrhizal fungi bacteria agent fertilizer on the spot; It is characterized in that described step 2) described in the host plant be onion or paspalum notatum, described bush mycorrhizal fungi is mould, the Glomus mosseae of sacculus in the root, children's cover sacculus is mould or one or more mixing thalline of native country bush mycorrhizal fungi.
4. farmland as claimed in claim 1 is the method for cultivating and producing bush mycorrhizal fungi bacteria agent fertilizer on the spot; It is characterized in that described step 2) described in host plant and the symbiote of bush mycorrhizal fungi reach 50-90% for host's root infection rate, spore count reaches the symbiote of 10-20/g.
5. farmland as claimed in claim 1 is the method for cultivating and producing bush mycorrhizal fungi bacteria agent fertilizer on the spot; It is characterized in that described step 2) in each week water the 15-25ml nutritive medium once; In every strain symbiote, add root simultaneously again and leach stoste 10-25ml; Compound amino-acid nutrition liquid 15-25ml, concentration is the ammonium humate solution 2-5ml of 1-3%
Described nutritive medium contains: KNO
3350-450mg/L, Ca (NO
3)
2550-600mg/L, MgSO
4200-300mg/L, H
3BO
34-5mg/L, MnCl
2.4H
2O 2-4mg/L, ZnSO
4.7H
2O 2-4mg/L, CuSO
4.5H
2O 0.1-0.5mg/L, H
2MoO
4.H
2O 0.1-0.5mg/L.
6. farmland as claimed in claim 1 is the method for cultivating and producing bush mycorrhizal fungi bacteria agent fertilizer on the spot, it is characterized in that nylon wire is 400 orders in the described step 3).
7. farmland as claimed in claim 1 is the method for cultivating and producing bush mycorrhizal fungi bacteria agent fertilizer on the spot; When it is characterized in that described step 3) mycelia carries out the absorption conversion of bio-feritlizer; Blade through cutting off part host plant or be 0.5% amino acid foliage fertilizer to host's plant leaf spray concentration, and water and execute the choline dichloride diluent.
8. farmland as claimed in claim 1 is the method for cultivating and producing bush mycorrhizal fungi bacteria agent fertilizer on the spot, adds concentration in the bush mycorrhizal fungi bacteria agent fertilizer that it is characterized in that obtaining in the described step 4) and is 1.0% glucose solution and leach stoste with root and be prepared into the farmland with cultivating fertilizer.
9. farmland as claimed in claim 8 is the method for cultivating and producing bush mycorrhizal fungi bacteria agent fertilizer on the spot, it is characterized in that described bush mycorrhizal fungi bacteria agent fertilizer, glucose solution and root leaching stoste three's weight ratio is 100: 0.5-5: 0.05-0.5.
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| CN108739319A (en) * | 2018-05-24 | 2018-11-06 | 南京农业大学 | A kind of method of molded substrates culturing vegetable |
| CN109906893A (en) * | 2019-03-18 | 2019-06-21 | 浙江师范大学 | A kind of method that Mycorrhizal higher yield of crops in farmland is high-quality |
| CN109906893B (en) * | 2019-03-18 | 2022-02-01 | 浙江师范大学 | Method for high yield and high quality of farmland mycorrhizal crops |
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| CN101805216A (en) | 2010-08-18 |
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