CN110229757A - One plant effectively facilitates the tangerine green trichoderma JS84 of plant growth and its biological organic fertilizer of development - Google Patents

One plant effectively facilitates the tangerine green trichoderma JS84 of plant growth and its biological organic fertilizer of development Download PDF

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Publication number
CN110229757A
CN110229757A CN201910545892.6A CN201910545892A CN110229757A CN 110229757 A CN110229757 A CN 110229757A CN 201910545892 A CN201910545892 A CN 201910545892A CN 110229757 A CN110229757 A CN 110229757A
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organic fertilizer
trichoderma
biological organic
tangerine green
green trichoderma
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CN201910545892.6A
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CN110229757B (en
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沈其荣
沈宗专
朱佳芯
李�荣
薛超
商美妮
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Nanjing Agricultural University
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Nanjing Agricultural University
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00Other organic fertilisers
    • C05F11/08Organic fertilisers containing added bacterial cultures, mycelia or the like
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/145Fungal isolates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/645Fungi ; Processes using fungi
    • C12R2001/885Trichoderma

Abstract

The invention discloses one plant to effectively facilitate the tangerine green trichoderma JS84 of plant growth and its biological organic fertilizer of development.A kind of tangerine green trichoderma JS84, is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and deposit number is CGMCC NO.17466, which being capable of a variety of Fusarium oxysporums such as antagonism banana, cucumber.The biological organic fertilizer is added obtained by function stem JS84 in common organic fertilizer, and viable count is preferably >=2.0 × 107CFU/g organic fertilizer dry weight.It is good to the growth-promoting effect of cucumber at seedling stage using the product, the high quality seedling that rhizosphere has a large amount of active function microorganisms can be brought out, the growth and yield after promoting kind of transplantation of seedlings.

Description

One plant effectively facilitates the tangerine green trichoderma JS84 of plant growth and its biology of development and has Machine fertilizer
Technical field
The invention belongs to field of agricultural microorganism, and in particular to one plant of tangerine green trichoderma JS84 for effectively facilitating plant growth and It is applied.
Background technique
Plant growth-promoting rhizobacteria (Plant Growth-promoting Rhizobacteria, PGPR), which refers to, is present in plant In object rhizosphere surface and root system surrounding soil microenvironment, micro- life of plant growth can be remarkably promoted by direct or indirect mode Object (Ahmad et al., Microbiological Research, 2008,163 (2): 173-181).Trichoderma is as PGPR's One kind can be effectively facilitated plant growth, at the same have it is low in cost, using safe, lasting effect is good, volume increase is stable, to ring The features such as border and food safety.Therefore, influence of the trichoderma to crop yield and growth traits is explored, can be Trichoderma preparation in green It is reasonable using scientific basis is provided in pollution-free vegetable production, there is important meaning to saving Fertilizer resources, Development of Organic Agriculture Justice.
Tangerine green trichoderma Trichoderma is disclosed in more documents (CN101637187A, CN105219650A) Citrinoviride can prevent and treat fungal diseases of plants, but there is not yet its growth-promoting functions relevant report.
Summary of the invention
It is an object of the invention to provide one plant and effectively facilitate crop life for the practical problem and demand in production practices Long tangerine green trichoderma JS84.
It is a further object of the present invention to provide the applications of the fungi.
It is yet another object of the invention to provide the bio-feritlizer of fungi preparation and its applications.
The purpose of the present invention can be achieved through the following technical solutions:
One plant, for promoting the growth-promoting bacterial strain JS84 of plant growth, is isolated from the agricultural land soil of Jiangsu, classification naming is tangerine Green trichoderma Trichoderma citrinoviride, is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms Center, the deposit date is on April 12nd, 2019, deposit number was CGMCC No.17466.
Bacterial strain JS84 is grown comparatively fast on PDA plate, radially grows mycelia, big volume production spore, and green spores produce spore cluster For white to green.The phylogenetic tree of bacterial strain ITS rDNA sequence construct analysis shows, bacterial strain JS84 is located at Trichoderma In citrinoviride branch, JS84 is accredited as tangerine green trichoderma in conjunction with physiological and biochemical test.
Tangerine green trichoderma JS84 of the present invention is promoting the application in plant growth.
Application of the tangerine green trichoderma JS84 of the present invention in the bio-feritlizer that preparation promotes plant growth.
The bio-feritlizer prepared by tangerine green trichoderma JS84 of the present invention.
The bio-feritlizer preferably mainly passes to lower section method preparation: the green wood of tangerine for being CGMCC No.17466 by deposit number Mould JS84 is inoculated into PDA liquid medium, is carried out liquid fermentation, and fermentation temperature is 28 DEG C, and mixing speed is 170 revs/min, Fermentation time is 96h, is fermented under illumination condition more excellent, and bacteria containing amount >=1 × 10 in fermentation liquid are made6A/mL.Every dry weight 100g stalk It is mixed with 200ml water, is added to 6.6ml amino acid and mixes thoroughly.115 DEG C of sterilizing 1h.After sterilization and cooling, 20% trichoderma of access fermentation Liquid is mixed thoroughly, is uniformly spread out in turnover box, after 28 DEG C are cultivated 4-5 days, is fabricated to trichoderma strain, is put in 4 DEG C of refrigerators and is saved. By the trichoderma strain of addition 5%~15% in organic fertilizer, production becomes trichoderma as biological organic fertilizer.PDA culture solution used is matched Method processed is, for preparing 1L culture medium: it is cut into small pieces to be put into water after being peeled with 200g potato and boils, 20min is boiled after boiling, Add 20g common glucose sugar in filtrate after filtering, is settled to 1000ml, pH value is naturally, 115 DEG C of sterilizing 30min.
Bio-feritlizer of the present invention is promoting the application in plant growth.
The utility model has the advantages that
The present invention mainly using the application for separating the tangerine green trichoderma that rhizosphere is effectively colonized, significantly improves crops seedling stage The speed of growth, promote crop nutrient in soil is absorbed and utilized, to promote plant growth.Promoting bacteria JS84 of the present invention exists The stabilization growth-promoting ability shown in more season pot experiments.Bio-feritlizer of the present invention is obvious using rear growth-promoting effect, is suitble to vegetables Crop producing region large area uses.
Detailed description of the invention
The phylogenetic tree that Fig. 1 uses adjacent method to establish based on the ITS rDNA gene order of bacterial strain JS84
Fig. 2 first season cucumber pot test effect figure
Fig. 3 second season cucumber pot test effect figure
Biomaterial preservation information
Bacterial strain JS84, classification naming are tangerine green trichoderma Trichoderma citrinoviride, are preserved in China Microbiological Culture presevation administration committee common micro-organisms center, preservation address are Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, China Institute of microbiology, the academy of sciences, the deposit date is on April 12nd, 2019, deposit number was CGMCC No.17466.
Specific embodiment
The separation and identification of embodiment 1, function stem
5g soil sample is placed in the triangular flask for filling 45ml sterile water, soil is made in 28 DEG C of oscillation 30min (170r/min) Suspension.It draws 0.1mL soil supension to be placed in trichoderma selectivity plate and be coated with, is placed in 28 DEG C of incubators and cultivates 48h, observe Bacterium colony growing state on plate selects the purifying of difference bacterium colony, 4 DEG C will be placed in strain inoculated to PDA culture medium after purification Refrigerator saves stand-by.
The trichoderma strain selected is inoculated in PDA culture medium, is respectively placed in 28 DEG C, 37 DEG C and 40 DEG C incubators, It is observed after culture 5d, filtering out can be in the high temperature trichoderma of 40 DEG C of normal growths.
It is named as JS84 by screening finally one plant of trichoderma of acquisition, bacterial strain JS84 bacterial strain JS84 on PDA plate is flat in PDA It is grown comparatively fast on plate, radially grows mycelia, big volume production spore, green spores, producing spore cluster is white to green.Bacterial strain ITS The phylogenetic tree of rDNA sequence construct analysis shows, bacterial strain JS84 is located in Trichoderma citrinoviride branch, JS84 is classified as Trichoderma citrinoviride, is preserved in China Committee for Culture Collection of Microorganisms Common micro-organisms center, deposit number are CGMCC No.17466.
The generation of embodiment 2, function bacterium bio-feritlizer
Bacterial strain JS84 (CGMCC No.17466) is seeded to liquid fermentation in PDA culture solution, the condition of fermenting and producing are as follows: Fermentation temperature is 28 DEG C, and mixing speed is 170 revs/min, and fermentation time 96h makes bacteria containing amount >=1 × 10 in fermentation liquid6A/ mL.Every dry weight 100g stalk and the mixing of 200ml water, are added to 6.6ml acidolysis aminoacids solution and (disclose in ZL201410042218.3 Liquid amino acid compound) mix thoroughly.115 DEG C of sterilizing 1h.After sterilization and cooling, 20% trichoderma fermentation liquid is accessed, is mixed thoroughly, is uniformly spread It is opened in turnover box, after 28 DEG C are cultivated 4-5 days, is fabricated to trichoderma strain, be put in 4 DEG C of refrigerators and save.By addition 5%~15% Trichoderma strain in organic fertilizer, production become trichoderma as biological organic fertilizer.
Wherein PDA used culture liquid making method is, for preparing 1L culture medium: being cut into after being peeled with 200g potato small Block is put into water and boils, and 20min is boiled after boiling, adds 20g common glucose sugar in filtrate after filtering, is settled to 1000ml, pH value is certainly So, 115 DEG C of sterilizing 30min.
Embodiment 3, potting growth-promoting effect test
Application effect of the 3.1 growth-promoting bio-fertilizers on cucumber (first season, the second season)
Growth-promoting pot experiment carries out in national organic fertilizer Engineering Technical Research Centre (Yixing) Greenhouse, for examination soil Earth is the healthy sandy soil without pathogen, is the rich cucumber of dew without pathogen for examination cucumber variety.Cucumber seeds are immersed in 1h in normal-temperature water is placed on wet gauze in 30 DEG C of incubators vernalization 12~for 24 hours, after the sprouting of 75% or more seed, It chooses the seed to show money or valuables one carries unintentionally and moves to seedlings nursing plate nursery, selected after growing 2 true leaves in the consistent seedling replanting basin of growing way.Test setting Two processing: 1) CK does not apply any fertilizer;2) OF is handled, common organic fertilizer is applied;3) JS84 is handled, trichoderma as biological is applied Organic fertilizer.2% common organic fertilizer is added in 6 basins of each processing, every basin dress 1.5kg soil, OF, and 2% trichoderma as biological is added in JS84 Organic fertilizer, Routine Management, the physical signs of the rear measurement plant of 30d or so.
First season cucumber growth-promoting effect such as Fig. 2, biomass situation are as shown in table 1, after potting culture 30d, application JS84 wood Plant height in cucumber plant of mould biological organic fertilizer processing (JS84), application of organic fertilizers (OF) processing and (CK) processing of not applying fertilizer, stem Slightly, there is significant difference in terms of overground part fresh weight and overground part dry weight.Compared with (CK) processing of not applying fertilizer, JS84 trichoderma is applied Biological organic fertilizer handles (JS84), and plant height, stem thickness, chlorophyll, overground part fresh weight and the overground part dry weight of cucumber plant increase respectively Add 17%, 8%, 5%, 18% and 36%.Compared with OF, apply JS84 trichoderma as biological organic fertilizer processing plant height, stem thickness, Chlorophyll, overground part fresh weight and overground part dry weight have increased separately 5%, 6%, 2%, 9% and 16%.
Second season cucumber growth-promoting effect such as Fig. 3, biomass situation are as shown in table 2, after potting culture 30d, application JS84 wood Plant height in cucumber plant of mould biological organic fertilizer processing (JS84), application of organic fertilizers (OF) processing and (CK) processing of not applying fertilizer, stem Slightly, there is significant difference in terms of chlorophyll, overground part fresh weight and overground part dry weight.Compared with CK, JS84 trichoderma as biological is applied The processing of organic fertilizer in terms of plant height, stem thickness, chlorophyll, overground part fresh weight and overground part dry weight, increased separately 60%, 30%, 11%, 58% and 41%.Compared with OF, apply JS84 trichoderma as biological organic fertilizer processing plant height, stem thickness, chlorophyll, In terms of overground part fresh weight and overground part dry weight, 30%, 11%, 3%, 23% and 18% has been increased separately.
To sum up the potting of two seasons the result shows that, with do not apply fertilizer (CK) processing and organic fertilizer (OF) handle cucumber in plant height, stem Slightly, difference is obvious between chlorophyll, overground part fresh weight and overground part dry weight, illustrates that the growth-promoting effect of JS84 bacterial strain is obvious.To sum up The potting of two seasons the result shows that, application JS84 trichoderma as biological organic fertilizer (JS84) processing cucumber plant growing way is not better than applying fertilizer at (CK) Reason and common organic fertilizer (OF) processing, and reach significant in terms of plant height, stem thickness, chlorophyll, overground part fresh weight, overground part dry weight Sex differernce shows that JS84 bacterial strain has apparent growth-promoting effect.
Table 1, first season cucumber growth-promoting pot experiment biomass situation
Table 2, second season cucumber potting growth-promoting test organism amount situation

Claims (7)

1. a kind of tangerine green trichoderma (Trichoderma citrinoviride) JS84 for effectively facilitating plant growth, it is preserved in State's Microbiological Culture Collection administration committee common micro-organisms center, preservation address are Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Number, Institute of Microorganism, Academia Sinica, the deposit date is on April 12nd, 2019, deposit number was CGMCC NO.17466.
2. tangerine green trichoderma JS84 described in claim 1 answering in the functional biological organic fertilizer that preparation promotes plant growth With.
3. a kind of functional biological organic fertilizer for effectively facilitating plant growth, it is characterised in that add right in common organic fertilizer It is required that obtained by the trichoderma solid spawn of tangerine green trichoderma JS84 described in 1.
4. the functional biological organic fertilizer of tangerine green trichoderma JS84 according to claim 3, it is characterised in that the function Viable count >=2.0 × 10 tangerine green trichoderma JS84 described in type trichoderma as biological organic fertilizer7CFU/g organic fertilizer dry weight, organic matter >= 45%, total nutrient (N+P2O5+K2O) > 5%, moisture≤30%.
5. functional biological organic fertilizer according to claim 3, it is characterised in that the tangerine green trichoderma JS84 solid bacterium Kind is prepared by following fermentation process:
The tangerine green trichoderma JS84 that deposit number is CGMCC NO.17466 is inoculated into PDA culture solution, production is carried out liquid fermentation, 28 DEG C of fermentation temperature range, mixing speed be 170 revs/min, fermentation time 96h, make bacteria containing amount >=1.0 in fermentation liquid × 108A/ml;Every dry weight 100g stalk and the mixing of 200ml water, are added to 6.6ml aminoacids solution and mix thoroughly, 115 DEG C of sterilizing 1h.Sterilizing After cooling, 20% trichoderma fermentation liquid is accessed, mixes thoroughly, uniformly spreads out in turnover box, after 28 DEG C are cultivated 4-5 days, is fabricated to wood Mould species are put in 4 DEG C of refrigerators and save.
6. functional biological organic fertilizer according to claim 3, it is characterised in that the crop is cucumber.
7. the preparation method of functional biological organic fertilizer as claimed in claim 3, it is characterised in that comprise the steps of:
(1) the tangerine green trichoderma JS84 trichoderma solid spawn that deposit number is CGMCC NO.17466 is prepared;
(2) common organic fertilizer is added by the 5%~15% of organic fertilizer dry weight in the trichoderma strain of step (1) preparation to be uniformly mixed Functional biological organic fertilizer makes wherein viable count >=2.0 × 107CFU/g organic fertilizer dry weight.
CN201910545892.6A 2019-06-23 2019-06-23 Trichoderma citrinoviride JS84 capable of effectively promoting crop growth and bio-organic fertilizer developed by trichoderma citrinoviride JS84 Active CN110229757B (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112159764A (en) * 2020-10-15 2021-01-01 西藏自治区农牧科学院农业质量标准与检测研究所 Trichoderma citrinoviride strain XZ0509 and application thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101637187A (en) * 2008-10-23 2010-02-03 中国农业科学院农业资源与农业区划研究所 Microbial inoculum for preventing and curing fungal diseases of plants
US20150368673A1 (en) * 2012-12-28 2015-12-24 Rengin ELTEM Production of trichoderma citrinoviride micropropagules as a biocontrol agent by means of an economical process
CN107142211A (en) * 2017-05-11 2017-09-08 沈阳农业大学 The tangerine green trichoderma Snef1910 and metabolite of a kind of killing root-knot nematode and application

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101637187A (en) * 2008-10-23 2010-02-03 中国农业科学院农业资源与农业区划研究所 Microbial inoculum for preventing and curing fungal diseases of plants
US20150368673A1 (en) * 2012-12-28 2015-12-24 Rengin ELTEM Production of trichoderma citrinoviride micropropagules as a biocontrol agent by means of an economical process
CN107142211A (en) * 2017-05-11 2017-09-08 沈阳农业大学 The tangerine green trichoderma Snef1910 and metabolite of a kind of killing root-knot nematode and application

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112159764A (en) * 2020-10-15 2021-01-01 西藏自治区农牧科学院农业质量标准与检测研究所 Trichoderma citrinoviride strain XZ0509 and application thereof

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