CN110012769B - Teak mycorrhizal light-matrix container seedling raising method - Google Patents

Teak mycorrhizal light-matrix container seedling raising method Download PDF

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CN110012769B
CN110012769B CN201910287331.0A CN201910287331A CN110012769B CN 110012769 B CN110012769 B CN 110012769B CN 201910287331 A CN201910287331 A CN 201910287331A CN 110012769 B CN110012769 B CN 110012769B
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cup
teak
seeds
water
light
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CN110012769A (en
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姜英
刘雄盛
李娟�
刘菲
戴菱
韦铄星
蒋燚
黄荣林
林建勇
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Guangxi Zhuang Autonomous Region Forestry Research Institute
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/40Cultivation of spawn
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • A01G24/12Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
    • A01G24/15Calcined rock, e.g. perlite, vermiculite or clay aggregates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/22Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
    • A01G24/25Dry fruit hulls or husks, e.g. chaff or coir
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/28Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B1/00Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
    • C05B1/02Superphosphates

Abstract

The invention discloses a teak mycorrhization light matrix container seedling culture method which comprises the specific steps of inoculation microbial inoculum propagation culture, seed collection, germination acceleration, light matrix cup preparation, cup inoculation, nursery stock cultivation and management, wherein after the teak seeds are subjected to germination acceleration, the obtained seedlings are planted in non-woven fabric three-dimensional cups filled with light matrixes, meanwhile, fungus inoculation is carried out to realize the mycorrhization of the nursery stocks, and then the high-quality teak container seedlings are obtained through the nursery stock cultivation and management. The method is favorable for enhancing the propagation and cultivation of teak seeds, and the mycorrhiza is favorable for enhancing the water and mineral absorption capacity of the teak root system, and improving the drought resistance and afforestation survival rate of plants.

Description

Teak mycorrhizal light-matrix container seedling raising method
Technical Field
The invention belongs to the technical field of plant seedling culture, and particularly relates to a teak mycorrhizal light matrix container seedling culture method.
Background
Teak wood (Tectorta grandisL, f.) is a semi-deciduous arbour of the genus Naringus of the family Verbenaceae, is one of the most famous and precious tree species in the world, has excellent material, straight and delicate texture, strong decay resistance, is not damaged by worms, is not easy to catch fire and is easy to process, thus being a high-grade material for manufacturing industries such as wharfs, ship manufacturing, buildings, roof trusses, carriages, bridges, furniture and the like. The teak is originally produced in India, Burma, Thailand, Laos and the like, is introduced and cultivated in provinces such as Hainan, Yunnan, Guangxi, Guangdong, Fujian and Taiwan in China, and is also used as an important stone mountain tree species for cultivation in Guangxi. However, at present, the cultivation scale of teak in China is small, a large amount of teak logs are imported from abroad every year, and in the international market, the teak becomes one of the protected commodity materials, so that a teak commodity forest must be vigorously developed to meet the increasing demand of the teak. However, the teak has the characteristics of high self-incompatibility rate, low free pollination seed setting rate and the like, so that the seed yield is low, the teak seed forest harvesting resources in China are very limited, how to breed seedlings by using limited teak seeds and build the forest needs to seek an efficient teak seedling culture method which is beneficial to improving the survival rate of afforestation, and the mycorrhization seedling culture technology can be just used for solving the problem.
Mycorrhiza is a union formed by mycorrhizal fungi hypha in soil and a nursery stock nutrition root system, and is a common plant symbiosis phenomenon in nature. The principle of action of this symbiotic system is that higher plants synthesize carbohydrates under photosynthesis for themselves and fungi, which absorb nutrients from the soil, including water and inorganic salts, to be transported to the host plants for use. The mycorrhiza not only participates in various physiological activities and biochemical processes of host plants, but also improves the physical and chemical properties of soil, enhances the air permeability of the soil and promotes the growth of plants through the growth and spread of exogenous or epitaxial hypha in the soil. The mycorrhization technology is a method for making nursery stock mycorrhizally by artificial inoculation, so that the beneficial effects of mycorrhiza on nursery stock are fully utilized, the method is favorable for enhancing the water and mineral absorption capacity of plant root systems, improving the drought resistance of plants, improving the survival rate of afforestation and the like, but related reports of applying the mycorrhization technology to the cultivation of teak seedlings are not found in the prior art.
Disclosure of Invention
Aiming at the defects, the invention provides a teak mycorrhizal light matrix container seedling culture method which is simple and convenient to operate, low in cost, good in quality of cultured teak seedlings, high in afforestation survival rate and easy to popularize.
The invention is realized by adopting the following technical scheme:
a teak mycorrhizal light matrix container seedling raising method comprises the following specific steps:
(1) and (3) inoculating microbial inoculum propagation culture: filling a sterilized substrate to 2/3 parts of a plastic basin, uniformly spreading 10-20 g of a pre-expanded strain sample on the sterilized substrate, then planting clover bulbs, covering the sterilized substrate with a thickness of 2cm, watering thoroughly, moving to an illumination room or a greenhouse for culturing for 3-5 months, cutting off the overground part and the main stem of the clover, reserving fine roots, cutting the fine roots, uniformly mixing the cut fine roots with the sterilized substrate, and airing to serve as an inoculation microbial inoculum for later use; the sterilization matrix is obtained by sterilizing crude river sand by water vapor at 120 ℃ for 20 minutes;
(2) seed collection: completely immersing the fruit of teak in lime water for 8-10 days, removing floating seeds, mashing the peel of the fruit subjected to embolization, taking out the seeds, but not breaking the seeds, and continuously immersing the fruit which is not subjected to embolization in the lime water for 1-2 days until the fruit is subjected to embolization, and taking out the seeds; the lime water is obtained by adding 1-2 g of quicklime into each liter of water and uniformly mixing;
(3) accelerating germination: in the month of 5, soaking and disinfecting the seeds collected in the step (2) for 30min by using potassium permanganate with the mass concentration of 0.5%, then washing for 2-3 min by using running water, uniformly sowing the seeds on a sand bed, compacting by using a wood board, covering fine yellow soil with the thickness of 1-2 cm on the seeds, watering thoroughly by using a fine sprayer, then using bamboo chips as arches, and covering a thin film on the bamboo chips for heat preservation; spraying water once in the morning and at night every day, germinating the seeds after half a month to obtain bud seedlings, and transplanting the bud seedlings into a cup after the bud seedlings grow to be 3-5 cm;
(4) preparing a light matrix cup: uniformly mixing turf, chaff, perlite and vermiculite according to a volume ratio of 3:3:2:2 to obtain a mixture A, uniformly mixing calcium superphosphate and the mixture A according to a weight ratio of 1% to obtain a light matrix, and filling the light matrix to 2/3 of a non-woven fabric three-dimensional cup to obtain a seedling container cup; spraying 800 times of carbendazim on the light matrix in the seedling container cup one day before the bud seedlings are transplanted into the cup for disinfection, and then covering a clean film for sealing for more than 12 hours for standby;
(5) cup feeding and inoculation: mixing 10-30-mesh yellow core soil with water to obtain pasty yellow core soil, pouring the inoculation microbial inoculum obtained in the step (1) into the pasty yellow core soil, and uniformly stirring and mixing the pasty yellow core soil and the inoculation microbial inoculum according to the volume ratio of 2:1 to obtain a mixture B; the bud seedlings in the step (3) are stained with the mixture B, and each bud seedling is stained with 4-8 g of the mixture B; punching a circular hole in the center of the seedling container cup sterilized in the step (4) by using a thin wood stick, wherein the size of the hole is just large enough to plant the bud seedlings into the hole; the bud seedlings are planted into the holes, light substrates are backfilled, the light substrates around the bud seedlings are lightly compacted, and the bud seedlings are tightly combined with the light substrates;
(6) seedling cultivation and management: watering thoroughly in the morning and evening respectively once in the first two days after inoculation, and then watering thoroughly when the water loss of the light matrix in the seedling container cup reaches 40-60% of the original water content; spraying fertilizer for the first time after inoculating in a cup for half a month, spraying 0.02g of conventional compound fertilizer aqueous solution on each sprout, spraying fertilizer for each half a month, increasing the fertilizer spraying amount by 0.005 g/time each time, and spraying thoroughly water and cleaning after spraying fertilizer for 15 minutes each time; and (5) when the bud seedlings grow to 45-55 cm, the bud seedlings can be outplanted for forestation.
Further, the pre-expanded strain in the step (1) is obtained by mixing the glomus minor species and the glomus mosseae species in equal volume.
Further, the specification of the plastic basin in the step (1) is that the diameter is 15-21 cm, and the height is 13.5-19 cm.
Further, the specification of the non-woven fabric three-dimensional cup in the step (4) is 20 × 20 cm.
Compared with the prior art, the technical scheme has the following beneficial effects:
1. the method adopts the mycorrhization technology to cultivate the teak container seedlings, is favorable for strengthening the propagation and cultivation of the tree seeds, and the mycorrhiza is favorable for enhancing the water and mineral absorption capacity of the teak root system, improving the drought resistance of plants and improving the survival rate of afforestation.
2. The light matrix prepared by the grass peat, the chaff, the perlite, the vermiculite and the calcium superphosphate is used for raising seedlings of teak, and the grass peat is rich in organic matters and has strong water holding capacity and water retention capacity; the husk contains nitrogen, phosphorus, potassium, calcium, etc.; the calcium superphosphate can adjust the pH value of the light matrix to adapt to the growth of teak seedlings, and simultaneously adjust the concentration of calcium elements in the light matrix, so that the absorption of major elements of nitrogen, phosphorus, potassium and magnesium and trace elements of iron, manganese, zinc, boron and copper in grass peat and rice husks by the teak seedlings can be promoted, therefore, the light matrix prepared by mixing the grass peat, the rice husks, perlite, vermiculite and the calcium superphosphate can supplement nutrients for the growth of the teak, the growth of the teak seedlings is effectively promoted, the prepared matrix is light in weight, the transportation of afforestation seedlings in rocky mountain areas is facilitated, the labor intensity is reduced, and the afforestation efficiency is improved.
3. According to the method, 4-8 g of a mixture of loess and an inoculation microbial inoculum is dipped in the root system of each teak bud seedling and then planted in a light matrix cup, so that each bud seedling can be fully inoculated with the microbial inoculum, the process of teak mycorrhization is accelerated, and the formation of teak mycorrhization is promoted.
4. The method scientifically manages and protects the teak seedlings, monitors the water content of the light matrix in the seedling container cup, and drenches when the water loss reaches 40-60% of the original water content, so that the humidity can be kept and the diseases caused by excessive wetting of the teak can be prevented; the regular and quantitative fertilization of teak seedlings is beneficial to promoting the robust growth of the seedlings.
Detailed Description
The invention is further illustrated by the following examples, which are not to be construed as limiting the invention thereto. The specific experimental conditions and methods not indicated in the following examples are generally conventional means well known to those skilled in the art.
Example 1:
a teak mycorrhizal light matrix container seedling raising method comprises the following specific steps:
(1) and (3) inoculating microbial inoculum propagation culture: filling a sterilized substrate to 2/3 parts of a plastic basin, uniformly spreading 10g of a pre-expanded strain sample on the sterilized substrate, then planting clover bulbs, covering the sterilized substrate with the thickness of 2cm, watering thoroughly, moving to an illumination room or a greenhouse for culturing for 3 months, cutting off the overground part and the main stem of the clover, keeping fine roots, cutting the fine roots, uniformly mixing the cut fine roots with the sterilized substrate, and airing to serve as an inoculation microbial agent for later use; the sterilization matrix is obtained by sterilizing crude river sand by water vapor at 120 ℃ for 20 minutes; the specification of the plastic basin is that the diameter is 18cm, and the height is 15.5 cm; the pre-expanded strain is obtained by mixing young sacculus japonicus species and mosisis sacculus fungus species in equal volume;
(2) seed collection: completely immersing the fruit of teak in lime water for 9 days, removing floating seeds, mashing the peel of the fruit subjected to embolization, taking out the seeds, but not breaking the seeds, and continuously immersing the fruit which is not subjected to embolization in the lime water for 1 day until the fruit subjected to embolization can be taken out; the lime water is obtained by adding 1g of quicklime into each liter of water and uniformly mixing;
(3) accelerating germination: in the month of 5, soaking and disinfecting the seeds collected in the step (2) for 30min by using potassium permanganate with the mass concentration of 0.5%, then washing for 2min by using running water, uniformly sowing the seeds on a sand bed, compacting by using a wood board, covering fine yellow soil with the thickness of 1cm on the seeds, watering thoroughly by using a fine sprayer, then using bamboo chips as arches, and covering a thin film on the bamboo chips for heat preservation; spraying water once in the morning and at night every day, germinating the seeds after half a month to obtain bud seedlings, and transplanting the bud seedlings into cups after the bud seedlings grow to 4 cm;
(4) preparing a light matrix cup: uniformly mixing turf, chaff, perlite and vermiculite according to a volume ratio of 3:3:2:2 to obtain a mixture A, uniformly mixing calcium superphosphate and the mixture A according to a weight ratio of 1% to obtain a light matrix, and filling the light matrix to 2/3 of a non-woven fabric three-dimensional cup to obtain a seedling container cup; spraying 800 times of carbendazim on the light matrix in the seedling container cup one day before the bud seedlings are transplanted into the cup for disinfection, and then covering a clean film for sealing for more than 12 hours for standby; the specification of the non-woven fabric three-dimensional cup is 20 multiplied by 20 cm;
(5) cup feeding and inoculation: mixing 10-30-mesh yellow core soil with water to obtain pasty yellow core soil, pouring the inoculation microbial inoculum obtained in the step (1) into the pasty yellow core soil, and uniformly stirring and mixing the pasty yellow core soil and the inoculation microbial inoculum according to the volume ratio of 2:1 to obtain a mixture B; staining the sprouts in the step (3) with the mixture B, wherein each sprout is stained with 4g of the mixture B; punching a circular hole in the center of the seedling container cup sterilized in the step (4) by using a thin wood stick, wherein the size of the hole is just large enough to plant the bud seedlings into the hole; the bud seedlings are planted into the holes, light substrates are backfilled, the light substrates around the bud seedlings are lightly compacted, and the bud seedlings are tightly combined with the light substrates;
(6) seedling cultivation and management: watering thoroughly in the morning and evening respectively for one time in the first two days after inoculation, and then watering thoroughly when the water loss of the light matrix in the seedling container cup reaches 60% of the original water content; spraying fertilizer for the first time after inoculating in a cup for half a month, spraying 0.02g of conventional compound fertilizer aqueous solution on each sprout, spraying fertilizer for each half a month, increasing the fertilizer spraying amount by 0.005 g/time each time, and spraying thoroughly water and cleaning after spraying fertilizer for 15 minutes each time; when the bud seedlings grow to 45cm, the seedlings can be outplanted for forestation.
The teak seedlings cultivated by the method are observed, mycorrhiza is formed after 3 months of cup inoculation of the bud seedlings, the requirements of outplanting and forestation are met after half a year, and the survival rate of the teak seedlings after transplantation reaches more than 98%.
Example 2:
a teak mycorrhizal light matrix container seedling raising method comprises the following specific steps:
(1) and (3) inoculating microbial inoculum propagation culture: filling a sterilized substrate to 2/3 parts of a plastic basin, uniformly spreading 20g of a pre-expanded strain sample on the sterilized substrate, then planting clover bulbs, covering the sterilized substrate with the thickness of 2cm, watering thoroughly, moving to an illumination room or a greenhouse for culturing for 4 months, cutting off the overground part and the main stem of the clover, keeping fine roots, cutting the fine roots, uniformly mixing the cut fine roots with the sterilized substrate, and airing to serve as an inoculation microbial agent for later use; the sterilization matrix is obtained by sterilizing crude river sand by water vapor at 120 ℃ for 20 minutes; the specification of the plastic basin is that the diameter is 21cm, and the height is 19 cm; the pre-expanded strain is obtained by mixing young sacculus japonicus species and mosisis sacculus fungus species in equal volume;
(2) seed collection: completely immersing the fruit of teak in lime water for 8 days, removing floating seeds, mashing the pericarp of the fruit subjected to embolization, taking out the seeds, but not breaking the seed kernels, and continuously soaking the fruit which is not subjected to embolization in the lime water for 2 days until the fruit subjected to embolization can be taken out; the lime water is obtained by adding 1.5g of quicklime into each liter of water and uniformly mixing;
(3) accelerating germination: in the month of 5, soaking and disinfecting the seeds collected in the step (2) for 30min by using potassium permanganate with the mass concentration of 0.5%, then washing for 3min by using running water, uniformly sowing the seeds on a sand bed, compacting by using a wood board, covering fine yellow soil with the thickness of 1.5cm on the seeds, watering thoroughly by using a fine sprayer, then using bamboo chips as arches, and covering a film on the bamboo chips for heat preservation; spraying water once in the morning and at night every day, germinating the seeds after half a month to obtain bud seedlings, and transplanting the bud seedlings into cups after the bud seedlings grow to 5 cm;
(4) preparing a light matrix cup: uniformly mixing turf, chaff, perlite and vermiculite according to a volume ratio of 3:3:2:2 to obtain a mixture A, uniformly mixing calcium superphosphate and the mixture A according to a weight ratio of 1% to obtain a light matrix, and filling the light matrix to 2/3 of a non-woven fabric three-dimensional cup to obtain a seedling container cup; spraying 800 times of carbendazim on the light matrix in the seedling container cup one day before the bud seedlings are transplanted into the cup for disinfection, and then covering a clean film for sealing for more than 12 hours for standby; the specification of the non-woven fabric three-dimensional cup is 20 multiplied by 20 cm;
(5) cup feeding and inoculation: mixing 10-30-mesh yellow core soil with water to obtain pasty yellow core soil, pouring the inoculation microbial inoculum obtained in the step (1) into the pasty yellow core soil, and uniformly stirring and mixing the pasty yellow core soil and the inoculation microbial inoculum according to the volume ratio of 2:1 to obtain a mixture B; staining the sprouts in the step (3) with the mixture B, wherein each sprout is stained with 6g of the mixture B; punching a circular hole in the center of the seedling container cup sterilized in the step (4) by using a thin wood stick, wherein the size of the hole is just large enough to plant the bud seedlings into the hole; the bud seedlings are planted into the holes, light substrates are backfilled, the light substrates around the bud seedlings are lightly compacted, and the bud seedlings are tightly combined with the light substrates;
(6) seedling cultivation and management: watering thoroughly in the morning and evening respectively for one time in the first two days after inoculation, and then watering thoroughly when the water loss of the light matrix in the seedling container cup reaches 40% of the original water content; spraying fertilizer for the first time after inoculating in a cup for half a month, spraying 0.02g of conventional compound fertilizer aqueous solution on each sprout, spraying fertilizer for each half a month, increasing the fertilizer spraying amount by 0.005 g/time each time, and spraying thoroughly water and cleaning after spraying fertilizer for 15 minutes each time; when the bud seedlings grow to 50cm, the bud seedlings can be outplanted for forestation.
The teak seedlings cultivated by the method are observed, mycorrhiza is formed after 3 months of cup inoculation of the bud seedlings, the requirements of outplanting and forestation are met after half a year, and the survival rate of the teak seedlings after transplantation reaches more than 98%.
Example 3:
a teak mycorrhizal light matrix container seedling raising method comprises the following specific steps:
(1) and (3) inoculating microbial inoculum propagation culture: loading a sterilized substrate to 2/3 parts of a plastic basin, uniformly spreading 15g of a pre-expanded strain sample on the sterilized substrate, then planting clover bulbs, covering the sterilized substrate with the thickness of 2cm, watering thoroughly, moving to an illumination room or a greenhouse for culturing for 5 months, cutting off the overground part and the main stem of the clover, keeping fine roots, cutting the fine roots, uniformly mixing the cut fine roots with the sterilized substrate, and airing to serve as an inoculation microbial agent for later use; the sterilization matrix is obtained by sterilizing crude river sand by water vapor at 120 ℃ for 20 minutes; the specification of the plastic basin is that the diameter is 15cm, and the height is 13.5 cm; the pre-expanded strain is obtained by mixing young sacculus japonicus species and mosisis sacculus fungus species in equal volume;
(2) seed collection: completely immersing the fruit of teak in lime water for 10 days, removing floating seeds, mashing the peel of the fruit subjected to embolization, taking out the seeds, but not breaking the seeds, and continuously soaking the fruit which is not subjected to embolization in the lime water for 2 days until the fruit subjected to embolization can be taken out; the lime water is obtained by adding 2g of quicklime into each liter of water and uniformly mixing;
(3) accelerating germination: in the month of 5, soaking and disinfecting the seeds collected in the step (2) for 30min by using potassium permanganate with the mass concentration of 0.5%, then washing for 2min by using running water, uniformly sowing the seeds on a sand bed, compacting by using a wood board, covering fine yellow soil with the thickness of 2cm on the seeds, watering thoroughly by using a fine sprayer, then using bamboo chips as arches, and covering a thin film on the bamboo chips for heat preservation; spraying water once in the morning and at night every day, germinating the seeds after half a month to obtain bud seedlings, and transplanting the bud seedlings into cups after the bud seedlings grow to be 3 cm;
(4) preparing a light matrix cup: uniformly mixing turf, chaff, perlite and vermiculite according to a volume ratio of 3:3:2:2 to obtain a mixture A, uniformly mixing calcium superphosphate and the mixture A according to a weight ratio of 1% to obtain a light matrix, and filling the light matrix to 2/3 of a non-woven fabric three-dimensional cup to obtain a seedling container cup; spraying 800 times of carbendazim on the light matrix in the seedling container cup one day before the bud seedlings are transplanted into the cup for disinfection, and then covering a clean film for sealing for more than 12 hours for standby; the specification of the non-woven fabric three-dimensional cup is 20 multiplied by 20 cm;
(5) cup feeding and inoculation: mixing 10-30-mesh yellow core soil with water to obtain pasty yellow core soil, pouring the inoculation microbial inoculum obtained in the step (1) into the pasty yellow core soil, and uniformly stirring and mixing the pasty yellow core soil and the inoculation microbial inoculum according to the volume ratio of 2:1 to obtain a mixture B; staining the sprouts in the step (3) with the mixture B, wherein each sprout is stained with 8g of the mixture B; punching a circular hole in the center of the seedling container cup sterilized in the step (4) by using a thin wood stick, wherein the size of the hole is just large enough to plant the bud seedlings into the hole; the bud seedlings are planted into the holes, light substrates are backfilled, the light substrates around the bud seedlings are lightly compacted, and the bud seedlings are tightly combined with the light substrates;
(6) seedling cultivation and management: watering thoroughly in the morning and evening respectively for one time in the first two days after inoculation, and then watering thoroughly when the water loss of the light matrix in the seedling container cup reaches 50% of the original water content; spraying fertilizer for the first time after inoculating in a cup for half a month, spraying 0.02g of conventional compound fertilizer aqueous solution on each sprout, spraying fertilizer for each half a month, increasing the fertilizer spraying amount by 0.005 g/time each time, and spraying thoroughly water and cleaning after spraying fertilizer for 15 minutes each time; when the bud seedlings grow to 55cm, the bud seedlings can be outplanted for forestation.
The teak seedlings cultivated by the method are observed, mycorrhiza is formed after 3 months of cup inoculation of the bud seedlings, the requirements of outplanting and forestation are met after half a year, and the survival rate of the teak seedlings after transplantation reaches more than 98%.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims (2)

1. A teak mycorrhizal light matrix container seedling raising method is characterized by comprising the following steps: the method comprises the following specific steps:
(1) and (3) inoculating microbial inoculum propagation culture: filling a sterilized substrate to 2/3 parts of a plastic basin, uniformly spreading 10-20 g of a pre-expanded strain sample on the sterilized substrate, then planting clover bulbs, covering the sterilized substrate with a thickness of 2cm, watering thoroughly, moving to an illumination room or a greenhouse for culturing for 3-5 months, cutting off the overground part and the main stem of the clover, reserving fine roots, cutting the fine roots, uniformly mixing the cut fine roots with the sterilized substrate, and airing to serve as an inoculation microbial inoculum for later use; the sterilization matrix is obtained by sterilizing crude river sand by water vapor at 120 ℃ for 20 minutes; the pre-expanded strain is obtained by mixing young sacculus japonicus species and mosisis sacculus fungus species in equal volume;
(2) seed collection: completely immersing the fruit of teak in lime water for 8-10 days, removing floating seeds, mashing the peel of the fruit subjected to embolization, taking out the seeds, but not breaking the seeds, and continuously immersing the fruit which is not subjected to embolization in the lime water for 1-2 days until the fruit is subjected to embolization, and taking out the seeds; the lime water is obtained by adding 1-2 g of quicklime into each liter of water and uniformly mixing;
(3) accelerating germination: in the month of 5, soaking and disinfecting the seeds collected in the step (2) for 30min by using potassium permanganate with the mass concentration of 0.5%, then washing for 2-3 min by using running water, uniformly sowing the seeds on a sand bed, compacting by using a wood board, covering fine yellow soil with the thickness of 1-2 cm on the seeds, watering thoroughly by using a fine sprayer, then using bamboo chips as arches, and covering a thin film on the bamboo chips for heat preservation; spraying water once in the morning and at night every day, germinating the seeds after half a month to obtain bud seedlings, and transplanting the bud seedlings into a cup after the bud seedlings grow to be 3-5 cm;
(4) preparing a light matrix cup: uniformly mixing turf, chaff, perlite and vermiculite according to a volume ratio of 3:3:2:2 to obtain a mixture A, uniformly mixing calcium superphosphate and the mixture A according to a weight ratio of 1% to obtain a light matrix, and filling the light matrix to 2/3 of a non-woven fabric three-dimensional cup to obtain a seedling container cup; spraying 800 times of carbendazim on the light matrix in the seedling container cup one day before the bud seedlings are transplanted into the cup for disinfection, and then covering a clean film for sealing for more than 12 hours for standby; the specification of the non-woven fabric three-dimensional cup is 20 multiplied by 20 cm;
(5) cup feeding and inoculation: mixing 10-30-mesh yellow core soil with water to obtain pasty yellow core soil, pouring the inoculation microbial inoculum obtained in the step (1) into the pasty yellow core soil, and uniformly stirring and mixing the pasty yellow core soil and the inoculation microbial inoculum according to the volume ratio of 2:1 to obtain a mixture B; the bud seedlings in the step (3) are stained with the mixture B, and each bud seedling is stained with 4-8 g of the mixture B; punching a circular hole in the center of the seedling container cup sterilized in the step (4) by using a thin wood stick, wherein the size of the hole is just large enough to plant the bud seedlings into the hole; the bud seedlings are planted into the holes, light substrates are backfilled, the light substrates around the bud seedlings are lightly compacted, and the bud seedlings are tightly combined with the light substrates;
(6) seedling cultivation and management: watering thoroughly in the morning and evening respectively once in the first two days after inoculation, and then watering thoroughly when the water loss of the light matrix in the seedling container cup reaches 40-60% of the original water content; spraying fertilizer for the first time after inoculating in a cup for half a month, spraying 0.02g of conventional compound fertilizer aqueous solution on each sprout, spraying fertilizer for each half a month, increasing the fertilizer spraying amount by 0.005 g/time each time, and spraying thoroughly water and cleaning after spraying fertilizer for 15 minutes each time; and (5) when the bud seedlings grow to 45-55 cm, the bud seedlings can be outplanted for forestation.
2. The teak mycorrhizal light matrix container seedling raising method according to claim 1, characterized in that: the specification of the plastic basin in the step (1) is that the diameter is 15-21 cm, and the height is 13.5-19 cm.
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