CN104126406A - Method for recovering vegetation in Karst stony desertification region quickly through arbuscular mycorrhiza technology - Google Patents
Method for recovering vegetation in Karst stony desertification region quickly through arbuscular mycorrhiza technology Download PDFInfo
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- CN104126406A CN104126406A CN201410385551.4A CN201410385551A CN104126406A CN 104126406 A CN104126406 A CN 104126406A CN 201410385551 A CN201410385551 A CN 201410385551A CN 104126406 A CN104126406 A CN 104126406A
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Abstract
The invention discloses a method for recovering vegetation in the Karst stony desertification region quickly through an arbuscular mycorrhiza technology. The method comprises the following steps that (1) arbuscular mycorrhizal fungi suitable for growing in the Karst region is chosen, such as glomus mosseae and glomus etunicatum; (2) arbuscular mycorrhiza host plants in the Karst region are chosen, trees which can be chosen are camphor trees, locust trees and paper mulberry, shrubs which can be chosen are honeysuckle and pyracantha, and herbs which can be chosen are clover and bidens; (3) arbuscular mycorrhizal fungi pellet seeds are adopted for the herbs, and mycorrhizal seedlings processed through a root dipping inoculation method are adopted for the trees and the locust trees; (4) in the beginning of herb sowing and tree and shrub transplant, water and fertilizer management and pest control are conducted reasonably to guarantee the rate of survival. According to the method, the arbuscular mycorrhiza technology is utilized, the three-dimensional planting mode combining the trees, the shrubs and the herbs is adopted, and the vegetation in the Karst stony desertification region can be recovered quickly.
Description
Technical field
The invention belongs to ecological recovery technical field, be specifically related to a kind of method of applying the fast quick-recovery of arbuscular mycorrhiza technology Karst Rocky Desertification Region vegetation.
Background technology
Karst In China landforms are widely distributed, more than type, for the world rare.In China, as the material base of Karst Landform Process In Mid---carbonate rock (as lime stone, dolomite, gypsum and rock salt etc.) distributes very wide.According to incompletely statistics, the gross area reaches 2,000,000 square kilometres, and wherein approximately 1,300,000 square kilometres of exposed carbonate rock areas, account for 1/7 of national total area; Approximately 700,000 square kilometres of the In Carbonate Rock areas burying.In the whole nation, all there is distribution each provinces and regions to In Carbonate Rock, but distribute the widest with osmanthus, He Dian eastern region, Guizhou Province.The ground such as the western Hunan, west place in Hubei, Chuan Dong, Shandong, Shanxi, the area that In Carbonate Rock distributes is also wider.The karst features of karst uniqueness, cause earth's surface drought and water shortage, soil to be run off by discontinuous, soil depletion, rich calcium meta-alkali water and soil serious etc., these limiting factors cause vegetation habitat harsh, biomass is less than normal, water, soil, plant interaction process have obvious fragility, finally show as easily damaged and difficult recovery.
Karst Rocky Desertification Region revegetation process is the reverse process of vegetation deterioration, and this process both can realize by natural deduction, also can realize by human intervention succession.Natural deduction is long required cycle time, and suitable human intervention can effectively shorten at succession initial stage required time, for spreading of stony desertification of containment raced against time.Arbuscular mycorrhizal fungi (the Arbuscular mycorrhizal fungus) For hundreds of millions of years of having survived in terrestrial soil, form mutualistic symbiosis body with 90% vascular plant on the earth, arbuscular mycorrhiza have promote plant to water and mineral element absorption, raising plant photosynthetic rate, help the formation of soil aggregate and strengthen the characteristics such as plant resistance to environment stress and patience, in arbuscular mycorrhizal fungi Karst Rocky Desertification Region revegetation process, play important impetus.
Summary of the invention
The present invention is directed to the revegetation of Karst Regions In Southwest China Rocky Desertification Region, a kind of easy operating is provided, practical, a kind of effective method of applying the fast quick-recovery of arbuscular mycorrhiza technology Karst Rocky Desertification Region vegetation.Its object is that auxiliary plant adapts to the special living environment of Rocky Desertification Region, strengthens the absorbing capacity of plant to water and mineral element, improves plant photosynthetic rate, strengthens plant resistance to environment stress and patience, improves soil structure, and contribute to vegetation to recover rapidly.
The method of the fast quick-recovery of application arbuscular mycorrhiza technology of the present invention Karst Rocky Desertification Region vegetation, comprises the following steps:
(1) arbuscular mycorrhiza microbial inoculum is prepared: select arbuscular mycorrhiza indigenous species according to Karst ecosystem, Glomus mosseae (
glomus mosseae) and Glomus etunicatum (
glomus etunicatum); Concrete grammar: expand numerously using clover as host plant, expand river sand and rendzinas mixture (river sand: rendzinas mass ratio is 1:3) that numerous matrix is high-temperature sterilization; Arbuscular mycorrhiza microbial inoculum is the Rhizosphere Soil that contains arbuscular mycorrhizal fungi spore (>=10/g soil) mycelia and infect root segment;
(2) host plant is prepared:
Arbor is selected: camphor tree (
cinnamomum camphora), locust tree (
robinia pseudoacacia) and paper mulberry (
broussonetia kaempferi);
Shrub is selected: honeysuckle (
lonicera japonica), fiery sour jujube (
pyracantha fortuneana);
Draft is selected: clover (
trifolium repens), beggar-ticks (
bidens bipinnata);
(3) Mycorrhizal processing: tree and shrubs adopts the Va Mycorrhiza Seedling that dips in the processing of root inoculation method, draft adopts mycorhiza pellet seed; Concrete processing method is as follows:
Dip in root inoculation method: by arbuscular mycorrhiza microbial inoculum and clear water by volume 1:5 proportional arrangement become microbial inoculum suspension, stirring is dispersed in water microbial inoculum, add wherein again fine earth, limit edged stirs, become the mud (rare denseness of mud is depending on the tender degree of children of shoot root) of certain viscosity, then after the transplanting shoot root after pruning being dipped in to mud, move into planting hole, earthing, waters after compacting;
Arbuscular mycorrhiza pellet seed: get arbuscular mycorrhiza microbial inoculum and a certain amount of concave convex rod (a kind of adhesive, adhering to microbial inoculum the surface of the seed as degree), add water, mix and stir with seed, microbial inoculum and seed are rolled into coccoid, dry;
(4) mode that adopts tall filling grass to combine in Rocky Desertification Region is carried out revegetation, plants, and retain as far as possible original herbaceous plant between tall filling; The planting density of arbor strain is that every 100 square metres of 4-7 are capable, every row 5-8 strain; The planting density of shrub strain is that every 100 square metres of 9-11 are capable, every row 9-11 strain, and shrub is isosceles triangle and plants.
(5) the transplanting initial stage, conventional water and fertilizer management and the extermination of disease and insect pest often be carried out, to ensure survival rate.
The present invention is compared with prior art, there is obvious beneficial effect, from above scheme, arbuscular mycorrhiza have promote plant to water and mineral element absorption, raising plant photosynthetic rate, help the formation of soil aggregate and strengthen the characteristics such as plant resistance to environment stress and patience, Mycorrhizal Technology plays the object of utilizing fast soil nutrient and raising water utilization, can effectively promote the fast quick-recovery of vegetation.Adopt tall mode of filling with careless three combinations to carry out revegetation, avoided the unicity of species, can realize in a short time vegetation covering.In choice of tree species, consider the object that Legume nodule plant plays biological nitrogen fixation and increases soil fertility, configuration liana shrub reduces the effect of the exposed rate of rock for the climbing up by holding on to property of tendril.Adopt different Mycorrhizal measures for different plants, tree and shrubs adopts the Va Mycorrhiza Seedling that dips in the processing of root inoculation method, and draft adopts mycorhiza pellet seed.In a word, the present invention's application arbuscular mycorrhizal fungi technology, adopts tall solid growth mode of filling with careless three combinations, can realize fast Karst Rocky Desertification Region revegetation, and simple to operate, is easy to promote.
Embodiment
Following examples are to further illustrate of the present invention.Implementing location is Typical Karst location, Ling Bangui township, the Southern Pass, the west of Guizhou Province, and the exposed rate 70% of rock, selects two 200m
2test sample prescription, arranges 1 200m
2contrast sample prescription.
Embodiment 1:
Arbuscular mycorrhiza Inoculant preparation: clover is expanded numerous as host, expanding numerous matrix is through 0.14Mpa, the rendzinas of 124 ~ 126 DEG C of continuous moist heat sterilization 1h and river sand (rendzinas: river sand is 3:1), supplement Hoagland ' s nutrient solution according to host's upgrowth situation in good time, Inoculant for contain arbuscular mycorrhizal fungi spore (>=10/g), the outer mycelia of root and infect the Rhizosphere Soil of root segment.
Plant is selected: arbor is selected camphor tree, paper mulberry, locust tree, and shrub is selected honeysuckle, fiery sour jujube, and draft is selected clover, and Qiao fills with the three-dimensional collocation of grass and plants.Nursery stock specification before plantation: camphor tree seedling (plant height 25cm, leading thread 0.40cm), paper mulberry seedling (plant height 23.5cm, leading thread 0.35cm), locust tree seedling (plant height 28cm, leading thread 0.41cm), honeysuckle cuttage seeding (plant height 30cm, hat width 20cm × 20cm, leading thread 0.5cm), fiery sour jujube seedling (plant height 20cm, leading thread 0.35cm).
Dip in root inoculation method: by arbuscular mycorrhiza microbial inoculum and clear water by volume 1:5 proportional arrangement become microbial inoculum suspension, stirring is dispersed in water microbial inoculum, add wherein again fine earth, limit edged stirs, become the mud (rare denseness of mud is depending on the tender degree of children of shoot root) of certain viscosity, then after the transplanting shoot root after pruning being dipped in to mud, move into planting hole, earthing, waters after compacting.
(seeding row spacing is 2.0m × 3.0m to arbor within experimental field, planting hole size is 20 cm × 20, cm × 20 cm), (seeding row spacing is 1.0m × 1.0m to shrub, planting hole size is 20 cm × 20, cm × 20 cm), isosceles triangle interval plantation Mycorrhizal arbor and shrub seedling, and within experimental field, broadcast sowing mycorhiza pellet seed.Control group is except adopting non-bacteriumization seedling and seed, and other conditions are all consistent with experimental group.
After 3 months, test plot herbaceous layer clover has covered all soil, average height 5cm; Camphor tree height 25.1cm, leading thread 0.41cm; Paper mulberry seedling: high 23.6cm, leading thread 0.36cm; Locust tree seedling: high 28.1cm, leading thread 0.41cm; Honeysuckle: high 35cm, hat width 25cm × 25cm, leading thread 0.52cm; Control sample ground herbaceous layer clover average height: 3cm; Camphor tree height 25 cm, leading thread 0.4cm; Paper mulberry seedling: high 23.55cm, leading thread 0.36cm; Locust tree seedling: high 28.06 cm, leading thread 0.40cm; Honeysuckle: high 32cm, hat width 22.5cm × 22cm, leading thread 0.51cm.
Test plot herbaceous layer clover average height 8cm after 6 months, clover total cover-degree reaches 85%; And have other more herbaceous plant to invade as beggar-ticks, cover degree 15%, perennial ryegrass cover degree 13%, spot thatch cover degree 25%; Camphor tree height 27cm, leading thread 0.5cm; Paper mulberry seedling: high 26cm, leading thread 0.40cm; Locust tree seedling: high 30cm, leading thread 0.5cm; Honeysuckle: high 50cm, hat width 30cm × 30cm, leading thread 0.6cm; Control sample ground herbaceous layer clover average height: 4cm; Camphor tree height 25.5 cm, leading thread 0.45cm; Paper mulberry seedling: high 24cm, leading thread 0.37cm; Locust tree seedling: high 29cm, leading thread 0.45cm; Honeysuckle: high 35cm, hat width 25cm × 25cm, leading thread 0.53cm.
After 1 year, test plot vegetation coverage reaches 70%.Herbaceous layer Plant Diversity is obviously improved, clover average height 8.5cm, clover always reaches cover degree 70% herbaceous layer, other herbaceous plant comprise beggar-ticks, perennial ryegrass, spot thatch cover degree, turn round Huang Mao, lance leaf hispid arthraxon, lemongrass, green foxtail, Artemisia japonica, creeping oxalis etc., account for 30% of whole sample prescription herbaceous layer; Camphor tree height 40cm, leading thread 1.2cm; Paper mulberry seedling: high 40cm, leading thread 1.5cm; Locust tree seedling: high 50cm, leading thread 1.5cm; Honeysuckle: high 145cm, hat width 60cm × 50cm, leading thread 1.3cm, stem of Flos Lonicerae spreads growth to bare rock, and overlying rock surface has reduced the exposed rate of rock.Control sample ground vegetation coverage reaches 40%, herbaceous layer clover average height: 5cm; Camphor tree height 30 cm, leading thread 0.6cm; Paper mulberry seedling: high 28cm, leading thread 0.5cm; Locust tree seedling: high 35cm, leading thread 0.65cm; Honeysuckle: high 65cm, hat width 35cm × 35cm, leading thread 0.65cm.
The above, it is only preferred embodiment of the present invention, not the present invention is done to any pro forma restriction, any technical solution of the present invention content that do not depart from, any simple modification, equivalent variations and the modification above embodiment done according to technical spirit of the present invention, all still belong in the scope of technical solution of the present invention.
Claims (7)
1. a method of applying the fast quick-recovery of arbuscular mycorrhiza technology Karst Rocky Desertification Region vegetation, comprises the following steps:
(1) arbuscular mycorrhiza microbial inoculum is prepared: select arbuscular mycorrhiza indigenous species according to Karst ecosystem;
(2) host plant is prepared: comprise arbor selection; Shrub is selected; Draft is selected;
(3) Mycorrhizal processing: tree and shrubs adopts and dips in the Va Mycorrhiza Seedling that root inoculation method was processed, and draft adopts arbuscular mycorrhiza pellet seed;
(4) mode that adopts tall filling grass to combine in Rocky Desertification Region is carried out revegetation, between tall filling, plants, and retains original herbaceous plant; The planting density of arbor strain is that every 100 square metres of 4-7 are capable, every row 5-8 strain; The planting density of shrub strain is that every 100 square metres of 9-11 are capable, every row 9-11 strain, and shrub is isosceles triangle and plants.
2. the method for the fast quick-recovery of application arbuscular mycorrhiza technology as claimed in claim 1 Karst Rocky Desertification Region vegetation, is characterized in that: in described step (1) arbuscular mycorrhiza bacterial classification comprise Glomus mosseae (
glomus mosseae) and Glomus etunicatum (
glomus etunicatum).
3. the method for the fast quick-recovery of application arbuscular mycorrhiza technology as claimed in claim 1 or 2 Karst Rocky Desertification Region vegetation, it is characterized in that: in described step (1), arbuscular mycorrhiza microbial inoculum preparation method: expand numerously using clover as host plant, expand river sand and rendzinas mixture (river sand: rendzinas mass ratio is 1:3) that numerous matrix is high-temperature sterilization; Arbuscular mycorrhiza microbial inoculum is the Rhizosphere Soil that contains arbuscular mycorrhizal fungi spore (>=10/g soil) mycelia and infect root segment.
4. the method for the fast quick-recovery of application arbuscular mycorrhiza technology as claimed in claim 1 Karst Rocky Desertification Region vegetation, is characterized in that: in described step (2), arbor is selected: camphor tree (
cinnamomum camphora), locust tree (
robinia pseudoacacia), paper mulberry (
broussonetia kaempferi); Shrub is selected: honeysuckle (
lonicera japonica), fiery sour jujube (
pyracantha fortuneana); Draft is selected: clover (
trifolium repens), beggar-ticks (
bidens bipinnata).
5. the method for the fast quick-recovery of application arbuscular mycorrhiza technology as claimed in claim 1 Karst Rocky Desertification Region vegetation, it is characterized in that: in described step (3), dip in root inoculation method: by arbuscular mycorrhiza microbial inoculum and clear water by volume 1:5 proportional arrangement become microbial inoculum suspension, stirring is dispersed in water microbial inoculum, then adds fine earth wherein, and limit edged stirs, become the mud of certain viscosity, then after the transplanting shoot root after pruning being dipped in to mud, move into planting hole, earthing, waters after compacting.
6. the method for the fast quick-recovery of the application arbuscular mycorrhiza technology Karst Rocky Desertification Region vegetation as described in claim 1 or 5, it is characterized in that: in described step (3), arbuscular mycorrhiza pellet seed method: get arbuscular mycorrhiza microbial inoculum and a certain amount of concave convex rod mixes, add water, mix and stir with seed, microbial inoculum and seed are rolled into coccoid, dry.
7. the method for the fast quick-recovery of application arbuscular mycorrhiza technology as claimed in claim 1 Karst Rocky Desertification Region vegetation, is characterized in that: in described step (4), at the transplanting initial stage, carry out conventional water and fertilizer management and the extermination of disease and insect pest, to ensure survival rate.
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| CN104480020A (en) * | 2014-11-12 | 2015-04-01 | 新疆维吾尔自治区和田蚕桑科学研究所 | Xinjiang desert mulberry biological bacterial agent and production technology thereof |
| CN104604386A (en) * | 2015-02-09 | 2015-05-13 | 中国科学院南京土壤研究所 | Method for repairing farmland soil polluted by cadmium through using combination of arbuscular mycorrhizal fungi and sedum plumbizincicola |
| CN105505796A (en) * | 2016-01-08 | 2016-04-20 | 华南师范大学 | Method for reducing honeysuckle cadmium accumulation and increasing honeysuckle yield through glomus versiforme berch |
| CN105580688A (en) * | 2016-01-08 | 2016-05-18 | 华南师范大学 | Method for reducing cadmium accumulation amount and increasing yield of honeysuckle through glomus mosseae |
| CN105660098A (en) * | 2016-01-08 | 2016-06-15 | 华南师范大学 | Method for reducing honeysuckle cadmium accumulation and increasing yield of honeysuckle by means of glomus intraradices |
| CN105794456A (en) * | 2016-03-22 | 2016-07-27 | 广西科技大学鹿山学院 | Method for repairing rocky desertification mountain land through interplanting of cornus wilsoniana, raspberry trees and ivy |
| CN106538216A (en) * | 2016-11-16 | 2017-03-29 | 邱逸奎 | Stony desertification comprehensive control area tree-planting method |
| CN106613125A (en) * | 2016-11-08 | 2017-05-10 | 深圳市铁汉生态环境股份有限公司 | Method for promoting side slope green retrieval through mycorrhiza inoculation |
| CN107509539A (en) * | 2017-08-28 | 2017-12-26 | 昆明学院 | In the method that root system of plant Arbuscular Mycorrhizal Fungi improves Ecological Slope stability |
| CN107574126A (en) * | 2017-09-04 | 2018-01-12 | 中国环境科学研究院 | Application of the AMF in karst adaptive plant growth-promoting |
| CN109479663A (en) * | 2019-01-05 | 2019-03-19 | 安徽泓森高科林业股份有限公司 | A kind of method of stony desertification ecological region planting locust tree |
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| CN113373067A (en) * | 2021-07-28 | 2021-09-10 | 贵州省烟草公司遵义市公司 | Compound microbial agent capable of promoting plant growth and application thereof |
| CN114208464A (en) * | 2021-12-14 | 2022-03-22 | 华南农业大学 | Arbuscular mycorrhizal fungi application device and method capable of recycling fertilizer |
| CN116171808A (en) * | 2022-11-10 | 2023-05-30 | 云南大学 | Method for repairing degraded grassland by combining arbuscular mycorrhizal fungi and bacteria |
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| CN104480020A (en) * | 2014-11-12 | 2015-04-01 | 新疆维吾尔自治区和田蚕桑科学研究所 | Xinjiang desert mulberry biological bacterial agent and production technology thereof |
| CN104604386A (en) * | 2015-02-09 | 2015-05-13 | 中国科学院南京土壤研究所 | Method for repairing farmland soil polluted by cadmium through using combination of arbuscular mycorrhizal fungi and sedum plumbizincicola |
| CN105580688B (en) * | 2016-01-08 | 2019-05-28 | 华南师范大学 | A method of honeysuckle Cd accumulation is reduced using Glomus mosseae and improves its yield |
| CN105505796B (en) * | 2016-01-08 | 2019-05-28 | 华南师范大学 | A method of honeysuckle Cd accumulation is reduced using Glomus versiforme and improves its yield |
| CN105660098A (en) * | 2016-01-08 | 2016-06-15 | 华南师范大学 | Method for reducing honeysuckle cadmium accumulation and increasing yield of honeysuckle by means of glomus intraradices |
| CN105660098B (en) * | 2016-01-08 | 2019-05-28 | 华南师范大学 | A method of honeysuckle Cd accumulation is reduced using Glomus intraradices and improves its yield |
| CN105580688A (en) * | 2016-01-08 | 2016-05-18 | 华南师范大学 | Method for reducing cadmium accumulation amount and increasing yield of honeysuckle through glomus mosseae |
| CN105505796A (en) * | 2016-01-08 | 2016-04-20 | 华南师范大学 | Method for reducing honeysuckle cadmium accumulation and increasing honeysuckle yield through glomus versiforme berch |
| CN105794456A (en) * | 2016-03-22 | 2016-07-27 | 广西科技大学鹿山学院 | Method for repairing rocky desertification mountain land through interplanting of cornus wilsoniana, raspberry trees and ivy |
| CN106613125A (en) * | 2016-11-08 | 2017-05-10 | 深圳市铁汉生态环境股份有限公司 | Method for promoting side slope green retrieval through mycorrhiza inoculation |
| CN106538216A (en) * | 2016-11-16 | 2017-03-29 | 邱逸奎 | Stony desertification comprehensive control area tree-planting method |
| CN107509539A (en) * | 2017-08-28 | 2017-12-26 | 昆明学院 | In the method that root system of plant Arbuscular Mycorrhizal Fungi improves Ecological Slope stability |
| CN107574126A (en) * | 2017-09-04 | 2018-01-12 | 中国环境科学研究院 | Application of the AMF in karst adaptive plant growth-promoting |
| CN109479663A (en) * | 2019-01-05 | 2019-03-19 | 安徽泓森高科林业股份有限公司 | A kind of method of stony desertification ecological region planting locust tree |
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| CN110012769B (en) * | 2019-04-11 | 2021-11-19 | 广西壮族自治区林业科学研究院 | Teak mycorrhizal light-matrix container seedling raising method |
| CN113373067A (en) * | 2021-07-28 | 2021-09-10 | 贵州省烟草公司遵义市公司 | Compound microbial agent capable of promoting plant growth and application thereof |
| CN114208464A (en) * | 2021-12-14 | 2022-03-22 | 华南农业大学 | Arbuscular mycorrhizal fungi application device and method capable of recycling fertilizer |
| CN116171808A (en) * | 2022-11-10 | 2023-05-30 | 云南大学 | Method for repairing degraded grassland by combining arbuscular mycorrhizal fungi and bacteria |
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Application publication date: 20141105 |