CN104604386A - Method for repairing farmland soil polluted by cadmium through using combination of arbuscular mycorrhizal fungi and sedum plumbizincicola - Google Patents
Method for repairing farmland soil polluted by cadmium through using combination of arbuscular mycorrhizal fungi and sedum plumbizincicola Download PDFInfo
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- CN104604386A CN104604386A CN201510065503.1A CN201510065503A CN104604386A CN 104604386 A CN104604386 A CN 104604386A CN 201510065503 A CN201510065503 A CN 201510065503A CN 104604386 A CN104604386 A CN 104604386A
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Abstract
The invention discloses a method for repairing farmland soil polluted by cadmium through using a combination of arbuscular mycorrhizal fungi and sedum plumbizincicola. The method comprises the steps that expanding propagation is conducted on arbuscular mycorrhizal fungi inoculant, and repair is conducted through inoculating the farmland soil with the inoculant to strengthen the sedum plumbizincicola. According to the method for repairing the farmland soil polluted by the cadmium through using the combination of the arbuscular mycorrhizal fungi and the sedum plumbizincicola, it is proved in the experiment that in the mode of normal field management, the growth of the sedum plumbizincicola can be promoted through inoculating the arbuscular mycorrhizal fungi inoculant, repair on the cadmium of the soil by the sedum plumbizincicola is improved, and the total amount of the extraction of the cadmium can be increased by 75% to a maximum.
Description
Technical field
The invention belongs to the restorative procedure in heavy metal cadmium farmland, be specifically related to utilize arbuscular mycorrhizal fungi to combine the biological reinforcing method of the restoring cadmium polluted agricultural land soil of companion's ore deposit red-spotted stonecrop.
Background technology
Cadmium (Cd) is source of heavy metal pollution maximum in current soil, and after environment is subject to cadmium pollution, cadmium can enrichment in vivo, as " cadmium rice ", enter human body by food chain, damage renal tubule and can the illnesss such as osteoporosis be caused, serious harm human health and ecological safety.Therefore, how to reduce heavy metal in soil cadmium content, ensure that food grain security becomes focus in recent years.Present cadmium pollution soil has multiple restorative procedure, comprising: physical measure, chemical measure, biological control measure, Ecological Measures etc.But utilize hyperaccumulative plant to carry out biological prosthetic feature with low cost with it, not spoiled soil ecology and non-secondary pollution, be subject to extensive concern gradually.
Companion ore deposit red-spotted stonecrop finds a kind of new zinc Cd hyperaccumulator in the recent period, and happiness is grown on is rich in Cd, the mining area of Zn.Its biomass is large, and fast growth is considered to have potential application prospect in reparation Cd contaminated soil.There are some researches show, in the soil of companion's ore deposit red-spotted stonecrop continuous repair 6 times, ammonium acetate bound residue cadmium and full dose cadmium are all decreased significantly.In order to improve the efficiency that companion's ore deposit red-spotted stonecrop heavy metal is repaired further, adopting the means of microorganism-plant combined, introducing arbuscular mycorrhizal fungi (Arbuscular mycorrhizal fungi is called for short AMF).AMF is the peculiar microorganism that a class is distributed widely in soil, it can with the root growth mycorhiza of majority of plants, promote the absorption of host plant mineral matter nutritional, be conducive to plant growth, improve Aboveground Biomass of Young, be applied to repair the more cadmium element of simultaneously stability being expected to reduce heavy metal on plants murder by poisoning.The reparation plantation that the present invention will select suitable mycorrhizal fungi to be applied to companion ore deposit red-spotted stonecrop, strengthening companion ore deposit red-spotted stonecrop, to cadmium remediation efficiency, provides technological service for Cd contaminated soil is biological prosthetic.Chinese invention patent (201410197384.0) has invented a kind of method utilizing mycorrhizal fungi Glomus versiforme to combine black nightshade repairing heavy metal pollution, but Solanum nigrum and root edible or be used as medicine, there is certain limitation as rehabilitation plant, use corn to prepare the microbial inoculum cycle long simultaneously.The reparation plantation that the present invention will select multiple mycorrhizal fungi to be applied to companion ore deposit red-spotted stonecrop, strengthening companion ore deposit red-spotted stonecrop, to cadmium remediation efficiency, provides new technological service for Cd contaminated soil is biological prosthetic.
Summary of the invention
In order to overcome the deficiencies in the prior art, the present invention is directed to heavy metal cadmium agricultural land soil, providing a kind of method utilizing arbuscular mycorrhizal fungi to combine the restoring cadmium polluted agricultural land soil of companion's ore deposit red-spotted stonecrop.The inventive method further increases cadmium over-accumulated plant companion ore deposit red-spotted stonecrop to the extraction of soil heavy metal cadmium, enhances its repairing effect to Cd-polluted farmland soil, utilizes the method can accelerate the Productivity restoration of Cd-polluted farmland soil.
Technical solution of the present invention specifically describes as follows.
The invention provides a kind of method utilizing arbuscular mycorrhizal fungi to combine the restoring cadmium polluted agricultural land soil of companion's ore deposit red-spotted stonecrop, the numerous strengthening with field soil inoculation microbial inoculum of expansion specifically comprising bush mycorrhizal fungi preparation accompanies ore deposit red-spotted stonecrop to repair step; Wherein: described bush mycorrhizal fungi preparation be light wall Acaulospora (
acaulospora laevis34), nectarine Acaulospora (
acaulospora mellea40) or Glomus mosseae (
glomus mosseae90107) any one in.
In the present invention, the expansion of described bush mycorrhizal fungi preparation is numerous, concrete steps are: river sand, soil, vermiculite, zeolite and the microbial inoculum ratio according to weight ratio 2:1:1:1:1 mixed, with clover or Sudan grass for host plant, Routine Management 2 ~ 3 months, collect plant roots, be cut into root and have no progeny and expand numerous matrix and mix, obtain bush mycorrhizal fungi preparation.
In the present invention, the concrete implementation step of described field soil inoculation microbial inoculum strengthening companion ore deposit red-spotted stonecrop reparation is: the soil region selecting cuttage companion ore deposit red-spotted stonecrop, opening 10 ~ 15cm is dark, 45 ~ 55g bush mycorrhizal fungi preparation is sprinkled in cave, cuttage enters the long companion ore deposit red-spotted stonecrop of 8 ~ 12cm, earthing compacting, field normal management 1.5 ~ 2.5 months, collects plant.
In the present invention, river sand, soil, vermiculite, zeolite all need high-temperature sterilization before using, and moist heat sterilization condition is 121 DEG C of sterilizing 40 ~ 60min, and after 18 ~ 24 hours, sterilizing is once again at interval.
In the present invention, companion red-spotted stonecrop soil cuttage region, ore deposit is: plantation wide 1.5 ~ 2 m in Gansu Province, Gansu Province long 3 ~ 4 m, plant line space 0.15 ~ 0.25 m, spacing in the rows 0.12 ~ 0.18 m.
In the present invention, soil region field normal management mode is: fertilizer is urea 12.5 kg/ mu.
The present invention has following beneficial effect:
Arbuscular mycorrhizal fungi can improve the absorption of host plant to soil nutrient, special phosphorus element; certain protective role can be played to host plant under heavy metal stress effect simultaneously; therefore; Arbuscular Mycorrhizal Fungi is of value to the upperground part biomass improving cadmium over-accumulated plant companion ore deposit red-spotted stonecrop; improve the extraction of the total cadmium of soil, thus reach the effect of fortification of plants reparation.
Accompanying drawing explanation
Fig. 1 Arbuscular Mycorrhizal Fungi is on the impact of companion ore deposit red-spotted stonecrop Aboveground Biomass of Young.
Fig. 2 Arbuscular Mycorrhizal Fungi is on the impact of cadmium concentration in the red-spotted stonecrop plant of companion ore deposit.
Fig. 3 arbuscular mycorrhizal fungi is on the impact of Cd uptake amount in the red-spotted stonecrop individual plants of companion ore deposit.
Embodiment
The present invention utilizes arbuscular mycorrhizal fungi to combine companion's ore deposit red-spotted stonecrop to carry out strengthening reparation to Cd-polluted farmland soil, and its concrete grammar is as follows:
(1) expansion of bush mycorrhizal fungi preparation is numerous
Bush mycorrhizal fungi preparation be light wall Acaulospora (
acaulospora laevis34), nectarine Acaulospora (
acaulospora mellea40) or Glomus mosseae (
glomus mosseae90107) any one in, is stored in Nanjing Soil Inst., Chinese Academy of Sciences; Expanding numerous host plant for bush mycorrhizal fungi preparation is clover (winter) or Sudan grass (other seasons).Expand numerous soil collecting from xiangtan, hunan province county heavy metal cadmium agricultural land soil for bush mycorrhizal fungi preparation, its cadmium pollution concentration is 0.68 mg/kg, exceedes national secondary environmental quality standards value.Its basic physical and chemical is pH 4.9, the soil organic matter 32 g/kg, total Phosphorus In Soil 0.51 g/kg, full nitrogen 1.48 g/kg, full potassium 11.2 g/kg, rapid available phosphorus 3.48 mg/kg, available nitrogen 91 mg/kg, available potassium 55 mg/kg, cation exchange capacity 10.4 cmol (+)/kg.2 mm sieves are crossed after soil specimen natural air drying, for subsequent use.
River sand, soil, vermiculite and zeolite etc. are carried out high-temperature heat sterilization, and sterilising conditions is 121 DEG C of sterilizings 1 hour, and after 24 hours, sterilizing is once, for subsequent use again at interval.The ratio of above-mentioned material according to mass ratio 2:1:1:1 is fully mixed, expands numerous matrix as microbial inoculum.In this mixed-matrix, access the microbial inoculum that 10%-15% contains arbuscular mycorrhizal fungi brood body, the water adding matrix weight about 20% after mixing thoroughly fills with through soil earth, sowing host plant seed.Normal management makes plant growth, can obtain the host plant that infected by arbuscular mycorrhizal fungi and the matrix containing arbuscular mycorrhizal fungi spore, the outer mycelia of root after 2 ~ 3 months.Cut off host plant acrial part, take out plant root segment and shred, root segment is mixed with soil matrix, be i.e. obtained bush mycorrhizal fungi preparation.
(2) field soil inoculation microbial inoculum strengthening companion ore deposit red-spotted stonecrop is repaired
Field trial is selected in heavy metal cadmium farmland, xiangtan, hunan province county, and (wide about 1.5 m in Gansu Province, Gansu Province is about 4.0 m), and in each community, according to planting distance, (about 0.2 m, spacing in the rows about 0.15 m) farmland to be divided into plantation community
Cuttage companion ore deposit red-spotted stonecrop (soil opening about 10 ~ 15cm is dark, chooses the companion ore deposit red-spotted stonecrop that length is about 10cm and carries out cuttage, earthing compacting).Simultaneously, the region of Arbuscular Mycorrhizal Fungi is set in community, separate with PVC round tube (diameter about 30 cm) and Soil Surrounding, Arbuscular Mycorrhizal Fungi microbial inoculum (being sprinkled into 50g bush mycorrhizal fungi preparation in each soil opening cave) in pvc pipe draws the line interior soil, then cuttage companion ore deposit red-spotted stonecrop.Earthing compacting, field normal management, about collects plant after 2 months, namely completes a repair process.
Daily field management: suitably water, fertilising: urea 12.5 kg/ mu.
(3) result of the test: on the result of the impact of biomass as shown in Figure 1, be vaccinated with for examination bush mycorrhizal fungi preparation, improve the growth of companion ore deposit red-spotted stonecrop, its upperground part biomass increases 67%(light wall Acaulospora respectively), 125%(nectarine Acaulospora) and 61%(Glomus mosseae); Meanwhile, inoculation nectarine Acaulospora significantly reduces the content of beary metal (reducing 22%) of plant, inoculates other two strain bush mycorrhizal fungi preparations also effective, but not as inoculation nectarine Acaulospora obvious (Fig. 2); Comprehensive, inoculate this three kinds of bush mycorrhizal fungi preparations, all make companion ore deposit red-spotted stonecrop can pluck part generally to be promoted (improve 47 ~ 75%) total extracted amount of Cadmium in Soil, in strengthening companion ore deposit red-spotted stonecrop cadmium reparation, there is potential using value (Fig. 3).
Claims (6)
1. utilize arbuscular mycorrhizal fungi to combine a method for the restoring cadmium polluted agricultural land soil of companion's ore deposit red-spotted stonecrop, its feature exists
In: specifically comprise the numerous and field soil inoculation microbial inoculum strengthening companion ore deposit red-spotted stonecrop of the expansion of bush mycorrhizal fungi preparation and repair step; Wherein: described bush mycorrhizal fungi preparation be light wall Acaulospora (
acaulospora laevis34), nectarine Acaulospora (
acaulospora mellea40) or Glomus mosseae (
glomus mosseae90107) any one in.
2., according to the method described in claim 1, it is characterized in that the expansion of described bush mycorrhizal fungi preparation is numerous,
Step is: river sand, soil, vermiculite, zeolite and the microbial inoculum ratio according to weight ratio 2:1:1:1:1 mixed, with clover or Sudan grass for host plant, Routine Management 2 ~ 3 months, collects plant roots, be cut into root have no progeny and expand numerous matrix and mix, obtain bush mycorrhizal fungi preparation.
3. according to the method described in claim 2, before it is characterized in that river sand, soil, vermiculite, zeolite use
All need high-temperature sterilization, moist heat sterilization condition is 121 DEG C of sterilizing 50 ~ 60min, and after 18 ~ 24 hours, sterilizing is once again at interval.
4., according to the method described in claim 1, it is characterized in that described field soil inoculation microbial inoculum strengthening companion
The concrete implementation step of ore deposit red-spotted stonecrop reparation is: the soil region selecting cuttage companion ore deposit red-spotted stonecrop, and opening 10 ~ 15cm is dark, is sprinkled in cave by 45 ~ 55g bush mycorrhizal fungi preparation, cuttage enters the long companion ore deposit red-spotted stonecrop of 8 ~ 12cm, earthing compacting, field normal management 1.5 ~ 2.5 months, collects plant.
5., according to the method described in claim 4, it is characterized in that companion red-spotted stonecrop soil cuttage region, ore deposit is: companion red-spotted stonecrop soil cuttage region, ore deposit is: plantation wide 1.5 ~ 2 m in Gansu Province, Gansu Province long 3 ~ 4 m, plant line space 0.15 ~ 0.25 m, spacing in the rows about 0.12 ~ 0.18 m.
6., according to the method described in claim 4, it is characterized in that soil region field normal management mode is:
Fertilizer is urea 12.5 kg/ mu.
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| CN105580688A (en) * | 2016-01-08 | 2016-05-18 | 华南师范大学 | Method for reducing cadmium accumulation amount and increasing yield of honeysuckle through glomus mosseae |
| CN106922243A (en) * | 2017-03-13 | 2017-07-07 | 兰州大学 | A kind of method that utilization AMF and flower slag improve soil quality |
| CN107127209A (en) * | 2017-07-17 | 2017-09-05 | 中国环境科学研究院 | A kind of method of antimicrobial plant renovation of heavy metal polluted soil with combined |
| CN108496599A (en) * | 2018-03-26 | 2018-09-07 | 贵州省生物研究所 | A kind of compound arbuscular mycorrhiza cuttage breeding method of trachelospermum jasminoide |
| CN109006242A (en) * | 2018-07-24 | 2018-12-18 | 贵州理工学院 | Between the practice joint AM fungi method that reduces cadmium, Zn content in capsicum and soil |
| CN109197385A (en) * | 2018-09-20 | 2019-01-15 | 浙江世佳科技有限公司 | A kind of arbuscular mycorrhizal fungi culture substrate |
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| CN105580688B (en) * | 2016-01-08 | 2019-05-28 | 华南师范大学 | A method of honeysuckle Cd accumulation is reduced using Glomus mosseae and improves its yield |
| CN105580688A (en) * | 2016-01-08 | 2016-05-18 | 华南师范大学 | Method for reducing cadmium accumulation amount and increasing yield of honeysuckle through glomus mosseae |
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| CN107127209A (en) * | 2017-07-17 | 2017-09-05 | 中国环境科学研究院 | A kind of method of antimicrobial plant renovation of heavy metal polluted soil with combined |
| CN108496599A (en) * | 2018-03-26 | 2018-09-07 | 贵州省生物研究所 | A kind of compound arbuscular mycorrhiza cuttage breeding method of trachelospermum jasminoide |
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| CN109845501A (en) * | 2019-01-31 | 2019-06-07 | 贵州省茶叶研究所 | A method of improving tea shoot field Mycorrhizal cutting propagation effect |
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| CN111618082B (en) * | 2020-04-22 | 2022-03-11 | 江苏省农业科学院 | Plant-microorganism synergistic shallow polluted soil treatment method and device |
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Application publication date: 20150513 |