CN112588800A - Remediation of ionic rare earth waste tailing area soil by using AM fungi and hybrid pennisetum alopecuroides - Google Patents
Remediation of ionic rare earth waste tailing area soil by using AM fungi and hybrid pennisetum alopecuroides Download PDFInfo
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- 239000002689 soil Substances 0.000 title claims abstract description 105
- 229910052761 rare earth metal Inorganic materials 0.000 title claims abstract description 48
- 150000002910 rare earth metals Chemical class 0.000 title claims abstract description 44
- 239000002699 waste material Substances 0.000 title claims abstract description 40
- 241000233866 Fungi Species 0.000 title claims abstract description 36
- 244000062720 Pennisetum compressum Species 0.000 title claims description 9
- 238000005067 remediation Methods 0.000 title description 11
- 241000209046 Pennisetum Species 0.000 claims abstract description 31
- 238000000034 method Methods 0.000 claims abstract description 26
- 244000064817 Sorghum halepense var. sudanense Species 0.000 claims abstract description 17
- 235000015503 Sorghum bicolor subsp. drummondii Nutrition 0.000 claims abstract description 15
- 238000005065 mining Methods 0.000 claims abstract description 14
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 12
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 11
- 238000009331 sowing Methods 0.000 claims abstract description 11
- 239000000126 substance Substances 0.000 claims abstract description 8
- 239000002344 surface layer Substances 0.000 claims abstract description 7
- 230000007613 environmental effect Effects 0.000 claims abstract description 5
- 230000035558 fertility Effects 0.000 claims abstract description 4
- 230000004720 fertilization Effects 0.000 claims abstract description 4
- -1 ion rare earth Chemical class 0.000 claims abstract description 4
- 230000000644 propagated effect Effects 0.000 claims abstract description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 20
- 241000196324 Embryophyta Species 0.000 claims description 18
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims description 12
- 230000012010 growth Effects 0.000 claims description 12
- 229910052757 nitrogen Inorganic materials 0.000 claims description 10
- 230000000694 effects Effects 0.000 claims description 9
- 235000015097 nutrients Nutrition 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 238000005516 engineering process Methods 0.000 claims description 7
- 230000008635 plant growth Effects 0.000 claims description 6
- 230000004083 survival effect Effects 0.000 claims description 6
- 230000000593 degrading effect Effects 0.000 claims description 5
- 230000002538 fungal effect Effects 0.000 claims description 4
- XSOHCQBMTDEBAD-UHFFFAOYSA-N azane Chemical compound N.N.N XSOHCQBMTDEBAD-UHFFFAOYSA-N 0.000 claims description 3
- 230000015572 biosynthetic process Effects 0.000 claims description 3
- 239000003344 environmental pollutant Substances 0.000 claims description 3
- 238000012165 high-throughput sequencing Methods 0.000 claims description 3
- 239000002054 inoculum Substances 0.000 claims description 3
- 231100000719 pollutant Toxicity 0.000 claims description 3
- 241000894007 species Species 0.000 claims description 3
- 238000009396 hybridization Methods 0.000 claims description 2
- 239000011159 matrix material Substances 0.000 claims description 2
- 230000001954 sterilising effect Effects 0.000 claims description 2
- 238000004659 sterilization and disinfection Methods 0.000 claims description 2
- 241001558929 Sclerotium <basidiomycota> Species 0.000 claims 2
- 238000005406 washing Methods 0.000 claims 1
- 230000008439 repair process Effects 0.000 abstract description 8
- 230000008929 regeneration Effects 0.000 abstract description 3
- 238000011069 regeneration method Methods 0.000 abstract description 3
- 230000018109 developmental process Effects 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 239000002028 Biomass Substances 0.000 description 2
- 241000272834 Cairina moschata Species 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000002386 leaching Methods 0.000 description 2
- ATRRKUHOCOJYRX-UHFFFAOYSA-N Ammonium bicarbonate Chemical compound [NH4+].OC([O-])=O ATRRKUHOCOJYRX-UHFFFAOYSA-N 0.000 description 1
- 229910000013 Ammonium bicarbonate Inorganic materials 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 235000012538 ammonium bicarbonate Nutrition 0.000 description 1
- 239000001099 ammonium carbonate Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 230000000855 fungicidal effect Effects 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000020477 pH reduction Effects 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 244000112814 pipewort Species 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C2101/00—In situ
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- Mycology (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Soil Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Cultivation Of Plants (AREA)
- Soil Conditioners And Soil-Stabilizing Materials (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention discloses a method for repairing ionic rare earth waste tailing area soil by using AM fungi and hybrid pennisetum, which comprises the following steps: (1) firstly, investigating AM fungi in the soil of the ionic rare earth waste tailing area; (2) detecting the physical and chemical properties of the soil in the ionic rare earth waste tailing area, discussing the soil fertility and main environmental influence factors, and evaluating the quality of the soil in the waste rare earth mining area; (3) carrying out propagation culture on AM fungus, namely Moxidou tube sacculus mildew by using Sudan grass; (4) ploughing and leveling the soil of the waste ion rare earth tailings mining area, and applying a propagated Moxidouguan sacculus mildew agent on the surface layer; (5) sowing hybrid pennisetum seeds on the soil applied with the microbial inoculum, and then covering soil; (6) and carrying out normal field management such as moisture management, fertilization management and the like. The invention can improve soil, increase vegetation coverage, promote resource regeneration and utilization rate and reduce repair cost.
Description
Technical Field
The invention relates to the field of ecology, and relates to a method for degrading ammonia nitrogen content in ionic rare earth waste ore area soil and improving pH value by utilizing the symbiotic action of AM fungi and hybrid pennisetum, in particular to a method for repairing ionic rare earth waste ore area soil by utilizing AM fungi and hybrid pennisetum.
Background
The ionic rare earth ore is an important strategic resource in China and is mainly distributed in Jiangxi, Guangdong, Fujian, Hunan, Guangxi and other areas in the south. For a long time, the ionic rare earth ore is mostly mined by adopting an in-situ ore leaching process such as ammonium sulfate, ammonium bicarbonate and the like, a large area of rare earth ore waste tailing land is left after mining, land resources are occupied, normal vegetation in a mining area and the periphery is damaged, the soil ammonia nitrogen content is high, the pH value is low, soil acidification and desertification are serious, the water and fertilizer retention capacity is weak, serious water and soil and nutrient loss is easily caused, the phenomenon of immortal weeds is caused, and the agricultural and economic development is seriously restricted. In addition, the content of mineral leaching agents such as ammonium sulfate and the like in the tailings land is high, and the surrounding water body and the farmland are polluted along with water and soil loss, surface runoff and other ways, so that the ecological environment and the human health are threatened. Therefore, the ecological restoration of the tailing land is not slow enough, and in order to accelerate the soil reconstruction and vegetation restoration of the tailing land, manual intervention must be introduced for restoring and treating the soil of the mining area.
At present, the soil remediation method mainly comprises a physical method, a chemical method and a biological method. The physical repair cost is high, the engineering quantity is large, the disturbance effect on soil is large, and the repair is not thorough; chemical agents such as catalysts used for chemical remediation are easy to pollute underground water and the like, and the consumed time is long; bioremediation can act by means of its own substances and energy, and does not carry a large amount of foreign substances into the environmental system, which is considered as the most economical and thorough ecological remediation technology in the remediation method. In the present day, on-site remediation by plants, microorganisms and natural remediation is the main method used for remediation of contaminated soil. Arbuscular mycorrhizal fungi (AM fungi) can form a symbiotic relationship with most plants, so that the plants can absorb nutrients such as nitrogen and phosphorus, and the AM fungi are combined with plant repair to serve as an economic and efficient repair technology and become a current research hotspot. On the basis, the hybrid pennisetum promotes the absorption of ammonia nitrogen in the ionic rare earth abandoned mining area soil through the AM fungal growth promotion effect, so that the ammonia nitrogen content in the degraded soil is achieved, the soil acidity is reduced, and the vegetation recovery of the rare earth tailing area soil is realized.
Disclosure of Invention
The invention aims to improve and repair the soil of ionic rare earth abandoned mining areas by utilizing a method for jointly repairing perennial gramineous plant hybrid pennisetum and AM fungus-Muscovy vessel sacculus mildew based on a plant and microorganism combined repair technology, improve the pH value of the soil, degrade the ammonia nitrogen content in the soil, further improve the vegetation coverage and restore the ecological structure. The method can accelerate the soil improvement period and reduce the cost under the growth promotion effect of the moccasin in Moxidou, has good restoration effect and no negative influence on the environment, and the harvested pennisetum alopecuroides can be used for papermaking and used as feed and can also be used as energy grass to provide biomass energy, so that the method not only can improve the soil and increase the vegetation coverage, but also can promote the resource regeneration and utilization rate and reduce the restoration cost.
In order to achieve the purpose, the invention provides the following technical scheme: the AM fungus and the hybrid pennisetum are utilized to repair the soil in the ionic rare earth waste tailing area:
the method for repairing the soil in the ionic rare earth waste tailing area by using AM fungi and hybrid pennisetum comprises the following steps:
(1) firstly, investigating AM fungi in the soil of the ionic rare earth waste tailing area;
(2) detecting the physical and chemical properties of the soil in the ionic rare earth waste tailing area, discussing the soil fertility and main environmental influence factors, and evaluating the quality of the soil in the waste rare earth mining area;
(3) carrying out propagation culture on AM fungus, namely Moxidou tube sacculus mildew by using Sudan grass;
(4) ploughing and leveling the soil of the waste ion rare earth tailings mining area, and applying a propagated Moxidouguan sacculus mildew agent on the surface layer;
(5) sowing hybrid pennisetum seeds on the soil applied with the microbial inoculum, and then covering soil;
(6) and carrying out normal field management such as moisture management, fertilization management and the like.
Preferably, the diversity of the AM fungi in the soil of the ionic rare earth waste tailing area is detected by utilizing a high-throughput sequencing technology in the step (1), dominant species with the largest proportion are obtained, and a larger amount of microbial inoculum is obtained by propagation culture.
Preferably, the sudangrass is used as a host plant in the step (3) to inoculate the madreponsia mossambica, a symbiotic relationship is established between the sudangrass and the sudangrass, and rhizosphere soil containing arbuscular mycorrhizal fungal spores, external root hyphae and root segments of the sudangrass is collected as an inoculant after three months of growth.
Preferably, the soil in the waste tailings area of the ionic rare earth is ploughed or repeatedly raked and smashed by a heavy rake so as to facilitate seed implantation, 170 kilograms of the expanded Muscovy tunica microbial inoculum is applied to the surface layer.
Preferably, the seeds of the hybrid pennisetum alopecuroides sowed in the step (5) are covered with 2 cm of soil, and 20-40 g or 200 seeds are sowed per square meter, so that more seeds can be sowed for ensuring the survival rate.
Preferably, watering is carried out 6-7 o' clock in the morning and evening after planting in the step (6), the maximum water holding capacity of the field is kept at 80%, watering is carried out once every 3-5 days after the seedlings come out of the earth, and watering is carried out once every month in the beginning and middle ten days after 3 months of growth; in order to enable the hybrid pennisetum to grow normally, Hoagland nutrient solution diluted by 10 times is sprayed after sowing, the Hoagland nutrient solution is sprayed once every other week, seedlings are sprayed at the root after emerging, AM fungi are inoculated at the roots of the hybrid pennisetum to promote the formation of inorganic nitrogen ammonium nitrogen in soil, nitrogen in the soil is converted into ammonium nitrogen which can be directly utilized by plant growth, nitrogen required by growth and development is provided for host plants, the effect of degrading nitrogen pollutants in the soil is further achieved, the pH value of the soil is finally increased to 6-7, the hybrid pennisetum is promoted to absorb the ammonium nitrogen in the soil, the plant growth is further promoted, and the vegetation coverage of ionic rare earth waste tailing areas is improved.
Preferably, the hybrid pennisetum seeds are sowed on the tailing ores with the applied microbial inoculum in the step (5), 20-40 g or 200 seeds are sowed per square meter, and more seeds can be sowed for ensuring the survival rate, and then 1 cm of soil is covered.
Preferably, the Sudan grass seeds are sown in the sterilized soil in the step (3), the seedlings are taken out after 1-2 weeks, the roots are washed clean, the Moxidouqinanensis sacculus mycorrhiza fungicide is inoculated to the roots of the seedlings, the seedlings are planted in a sterilized substrate for propagation culture for three months to obtain a rich culture, and rhizosphere soil containing Moxidouqinanensis sacculus mycosporium spores, ectorhizomyces and infected host plant root segments is taken as an inoculating agent to be stored in a refrigerator at 4 ℃.
Compared with the prior art, the invention has the beneficial effects that:
the method is based on a plant and microorganism combined remediation technology, and is used for improving and remedying the ionic rare earth abandoned mining area soil by utilizing a method of combining remediation of perennial gramineous hybrid pennisetum and AM fungi-Muxidou Tubulus Tulobus, improving the pH of the soil, degrading the ammonia nitrogen content in the soil, further improving the vegetation coverage and restoring the ecological structure. The method can accelerate the soil improvement period and reduce the cost under the growth promotion effect of the moccasin in Moxidou, has good restoration effect and no negative influence on the environment, and the harvested pennisetum alopecuroides can be used for papermaking and used as feed and can also be used as energy grass to provide biomass energy, so that the method not only can improve the soil and increase the vegetation coverage, but also can promote the resource regeneration and utilization rate and reduce the restoration cost.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides a technical scheme that: utilize AM fungus and hybridization chinese pennisetum to restore ion type rare earth abandonment tailing district soil, its characterized in that: the method comprises the following steps:
(1) firstly, investigating AM fungi in the soil of the ionic rare earth waste tailing area;
(2) detecting the physical and chemical properties of the soil in the ionic rare earth waste tailing area, discussing the soil fertility and main environmental influence factors, and evaluating the quality of the soil in the waste rare earth mining area;
(3) carrying out propagation culture on AM fungus, namely Moxidou tube sacculus mildew by using Sudan grass;
(4) ploughing and leveling the soil of the waste ion rare earth tailings mining area, and applying a propagated Moxidouguan sacculus mildew agent on the surface layer;
(5) sowing hybrid pennisetum seeds on the soil applied with the microbial inoculum, and then covering soil;
(6) and carrying out normal field management such as moisture management, fertilization management and the like.
Specifically, the diversity of the AM fungi in the soil of the ionic rare earth waste tailing area is detected by utilizing a high-throughput sequencing technology in the step (1), dominant species with the largest proportion are obtained, and a larger amount of microbial inoculum is obtained by propagation culture.
Specifically, the Sudan grass is used as a host plant to inoculate the Mossi pipewort, a symbiotic relationship is established between the Sudan grass and the host plant, and rhizosphere soil containing arbuscular mycorrhizal fungal spores, external root hyphae and root segments of the Sudan grass plant is collected as an inoculant after three months of growth.
Specifically, the soil in the waste tailings area of the ionic rare earth is ploughed or repeatedly raked and smashed by a heavy rake to facilitate seed nidation, and 170 kilograms of the expanded Muscovy tunica mycoides is applied to each mu of the surface layer.
Specifically, the seeds of the hybrid pennisetum alopecuroides sowed in the step (5) are covered with 2 cm of soil, and 20-40 g or 200 seeds are sowed per square meter, so that more seeds can be sowed for ensuring the survival rate.
Specifically, watering is carried out 6-7 o' clock in the morning and evening after planting in the step (6), the maximum water holding capacity of the field is kept at 80%, watering is carried out once every 3-5 days after the seedlings come out of the earth, and watering is carried out once every month in the beginning and middle ten days after 3 months of growth; in order to enable the hybrid pennisetum to grow normally, Hoagland nutrient solution diluted by 10 times is sprayed after sowing, the Hoagland nutrient solution is sprayed once every other week, seedlings are sprayed at the root after emerging, AM fungi are inoculated at the roots of the hybrid pennisetum to promote the formation of inorganic nitrogen ammonium nitrogen in soil, nitrogen in the soil is converted into ammonium nitrogen which can be directly utilized by plant growth, nitrogen required by growth and development is provided for host plants, the effect of degrading nitrogen pollutants in the soil is further achieved, the pH value of the soil is finally increased to 6-7, the hybrid pennisetum is promoted to absorb the ammonium nitrogen in the soil, the plant growth is further promoted, and the vegetation coverage of ionic rare earth waste tailing areas is improved.
Specifically, the hybrid pennisetum seeds are sowed on the tailing ores with the applied microbial inoculum in the step (5), 20-40 g or 200 seeds are sowed per square meter, and in order to ensure the survival rate, more seeds can be sowed, and then 1 cm of soil is covered.
Specifically, the Sudan grass seeds are sown in the sterilized soil in the step (3), the seedlings are taken out after 1-2 weeks, the roots are washed clean, the Moxidouqinanensis sacculus mycorrhizal fungi agent is inoculated to the roots of the seedlings, the seedlings are planted in a sterilization matrix for propagation culture for three months to obtain a rich culture, and rhizosphere soil containing Moxidouqinanensis sacculus mycosporium spores, ectorhizomyces and infected host plant root segments is taken as an inoculation agent and stored in a refrigerator at 4 ℃.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. Utilize AM fungus and hybridization chinese pennisetum to restore ion type rare earth abandonment tailing district soil, its characterized in that: the method comprises the following steps:
(1) firstly, investigating AM fungi in the soil of the ionic rare earth waste tailing area;
(2) detecting the physical and chemical properties of the soil in the ionic rare earth waste tailing area, discussing the soil fertility and main environmental influence factors, and evaluating the quality of the soil in the waste rare earth mining area;
(3) carrying out propagation culture on AM fungus, namely Moxidou tube sacculus mildew by using Sudan grass;
(4) ploughing and leveling the soil of the waste ion rare earth tailings mining area, and applying a propagated Moxidouguan sacculus mildew agent on the surface layer;
(5) sowing hybrid pennisetum seeds on the soil applied with the microbial inoculum, and then covering soil;
(6) and carrying out normal field management such as moisture management, fertilization management and the like.
2. The method for repairing the soil in the ionic rare earth waste tailing area by using the AM fungus and the hybrid pennisetum according to claim 1, is characterized in that: the method comprises the following steps of (1) detecting the diversity of AM fungi in the soil of the ionic rare earth waste tailing area by using a high-throughput sequencing technology to obtain dominant species with the largest proportion, and performing propagation culture to obtain a large amount of microbial inoculum.
3. The method for repairing the soil in the ionic rare earth waste tailing area by using the AM fungus and the hybrid pennisetum according to claim 1, is characterized in that: and (3) inoculating the Sclerotium moellendorfii hieron by using the Sudan grass as a host plant, establishing a symbiotic relationship between the Sclerotium moellendorfii hieron and the Sudan grass, and collecting rhizosphere soil containing arbuscular mycorrhizal fungal spores, external root hyphae and root segments of the Sudan grass plant as an inoculant after three months of growth.
4. The method for repairing the soil in the ionic rare earth waste tailing area by using the AM fungus and the hybrid pennisetum according to claim 1, is characterized in that: and (4) ploughing the soil of the waste tailings area of the ionic rare earth or repeatedly raking and raking the soil by using a heavy rake to facilitate seed nidation, applying the expanded-propagation Tunica moccasia agent on the surface layer, and applying 170 kg of the expanded-propagation Tunica agent per mu.
5. The method for repairing the soil in the ionic rare earth waste tailing area by using the AM fungus and the hybrid pennisetum according to claim 1, is characterized in that: and (5) covering soil for 2 cm after the seeds of the hybrid pennisetum alopecuroides are sowed, sowing 20-40 g or 200 seeds per square meter, and sowing more seeds for ensuring the survival rate.
6. The method for repairing the soil in the ionic rare earth waste tailing area by using the AM fungus and the hybrid pennisetum according to claim 1, is characterized in that: watering at 6-7 points in the morning and evening after planting in the step (6) once a day to keep the maximum water holding capacity of the field at 80%, watering the seedlings once every 3-5 days after the seedlings emerge, and watering the seedlings once in the early and middle months after 3 months of growth; in order to enable the hybrid pennisetum to grow normally, Hoagland nutrient solution diluted by 10 times is sprayed after sowing, the Hoagland nutrient solution is sprayed once every other week, seedlings are sprayed at the root after emerging, AM fungi are inoculated at the roots of the hybrid pennisetum to promote the formation of inorganic nitrogen ammonium nitrogen in soil, nitrogen in the soil is converted into ammonium nitrogen which can be directly utilized by plant growth, nitrogen required by growth and development is provided for host plants, the effect of degrading nitrogen pollutants in the soil is further achieved, the pH value of the soil is finally increased to 6-7, the hybrid pennisetum is promoted to absorb the ammonium nitrogen in the soil, the plant growth is further promoted, and the vegetation coverage of ionic rare earth waste tailing areas is improved.
7. The method for repairing the soil in the ionic rare earth waste tailing area by using the AM fungus and the hybrid pennisetum according to claim 1, is characterized in that: and (5) sowing hybrid pennisetum seeds on the tailing ores applied with the microbial inoculum, wherein 20-40 g or 200 seeds are sown per square meter, and the seeds can be sown more than one time in order to ensure the survival rate, and then covered with 1 cm of soil.
8. The method for repairing the soil in the ionic rare earth waste tailing area by using the AM fungus and the hybrid pennisetum according to claim 1, is characterized in that: and (3) sowing Sudan grass seeds in the sterilized soil, taking out seedlings after 1-2 weeks, washing the roots clean, inoculating the Moxidoudouqinanensis sacculus fungus agent to the roots of the seedlings, planting the seedlings in a sterilization matrix, performing propagation culture for three months to obtain a rich culture, and taking rhizosphere soil containing Moxidouqinanensis sacculus fungus spores, ectorhizomyces and infected host plant root segments as an inoculating agent to be stored in a refrigerator at 4 ℃.
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| CN112930743A (en) * | 2021-02-18 | 2021-06-11 | 江西省生态环境科学研究与规划院 | Method for restoring soil of ionic rare earth abandoned mining area |
| CN113560333A (en) * | 2021-07-06 | 2021-10-29 | 天津市滨海新区环境创新研究院 | Method for restoring ionic rare earth tailings soil |
| WO2022174564A1 (en) * | 2021-02-18 | 2022-08-25 | 江西省生态环境科学研究与规划院 | Repair material and repair method of ionic rare earth mine tailing area, and application |
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