CN108160685A - A kind of method that plant-microorganism joint repairs coal mining area soil - Google Patents
A kind of method that plant-microorganism joint repairs coal mining area soil Download PDFInfo
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- CN108160685A CN108160685A CN201810000526.8A CN201810000526A CN108160685A CN 108160685 A CN108160685 A CN 108160685A CN 201810000526 A CN201810000526 A CN 201810000526A CN 108160685 A CN108160685 A CN 108160685A
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C2101/00—In situ
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Soil Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Botany (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention belongs to geobiont repair technical fields, and in particular to the method for repairing coal mining area soil.A kind of method that phytomicroorganism joint repairs coal mining area soil, Trisetum bifidum seed by being exposed to the sun, soaking seed, germinateing by this method, the grass seeds after germination is seeded in emergence, nursery in the hole tray for be loaded with seedling medium again, its is after-applied by Acidithiobacillus ferrooxidans strain GF, rainbow conk keyhole bacterium, the secondary hat mould mix bacterium agent formed of sacculus, after contaminated soil in mining areas is smooth, improvement, seedling is embedded in ditch after ditching and to return soil and cover seedling, through field management, growth is gathered in after 90 120 days.By this method, after one or many plantations, the physical and chemical index of coal mining area soil can significantly improve, and reach arm's length standard, and the soil remediation for vast coal mining area provides new approach, has broad application prospects.
Description
Technical field
The invention belongs to geobiont repair technical fields, and in particular to the method for repairing coal mining area soil.
Background technology
Coal has higher accounting always in the energy-consuming in China, although with Green Development theory and 2016
Year coal industry actively dissolves the implementation of the policies such as superfluous production capacity so that coal is continuous 5 years in primary energy consumes accounting
It is on a declining curve, 62.3% in 2016 is down to from 70.2% in 2010, still, within following quite long-term, China is still
It is the energy resource structure based on coal.During the utilization of coal, inevitably ecology of mining areas is generated safely
It destroys, is in particular in the pollution of soil and damage, the pollution of water body and damage, atmosphere pollution, noise pollution, living resources
Damage, the damage of mining area ecological environment etc., the heavy metal pollution of soil is exactly one of them main aspect.Coal field soil
Heavy metal in earth is mainly derived from 3 aspects：（1）The dust migration sedimentation generated in process of coal mining, acts in wind-force
Under, Mine Dust is made to be re-distributed in coal mine surrounding soil, is entered in soil, is increased to surrounding by leaching diafiltration
The pollution of soil；（2）Containing the higher heavy metal of concentration in the gangue that coal mining generates, big in gangue stacking process
Gas precipitation is washed away and enters in soil with rainwash under eluviation or air is suspended in the form of airborne dust through eroding
In, in the final soil to land around Gangue；（3）Have to ore and country rock acid compared with highly dissoluble and rodent coal mine
Waste water can aggravate the dissolving of heavy metal in ore and country rock, and carry the harmful chemicals such as plurality of heavy metal and enter water body, dirty
Contaminate irrigation soils.For the big province of the producing coal in China, such as Shanxi Province, Shaanxi Province, Inner Mongolia Autonomous Region etc., coal mine surrounding area
Environment, soil regime are all received and are seriously affected, and heavy metal content in soil is high, type is more, and soil almost loses production capacity, has
Soil allow to Planting Crops, also due to heavy metal is penetrated into and is caused in crops containing plurality of heavy metal ingredient, such as
Fruit enters human body by food chain, and harm is inconceivable for caused by health, and the life and health of nearby residents is caused
Great threat.Therefore, coal mining area soil reparation and administer it is imperative.
Existing coal field soil remediation administering method mainly has physical chemistry reparation, biological prosthetic, further includes in grinding
Study carefully the clay reparation in stage.Physical chemistry reparation mainly elutes soil, chemosetting or electro reclamation etc., these
Technology is not only of high cost, should not carry out on a large scale, and can bring the reduction of soil activation, although preventing heavy metal pollution
Sprawling, but do not play a role in improving to the recovery of soil, therefore application is also few.And in recent years, it is biological prosthetic to be ground
The favor for the person of studying carefully.Bioremediation technology mainly improves soil physical chemistry using the biology in system or the metabolic activity of microorganism
Property improves biological activity of soil, so as to which ecological environment of soil be made to be restored to the process of reset condition partially or completely.Specifically
To for the reparation of coal mining area soil, mainly by planting the strong plant of some Metal uptake abilities, microorganism in dirt
Contaminate in area so that heavy metal is detached with soil, so as to reduce pollution, restore soil, though this measure is effective, often it is time-consuming compared with
It is long.Patent CN106607454A discloses a kind of Subsidence Land in Coal Mining Area chromium-polluted soil restorative procedure, first using grid multipoint method
Collecting soil sample is carried out, then the chromium content of the pedotheque to being acquired is measured, and is measured according to soil Cr content
As a result by soil divided rank, the soil on chromium-polluted soil surface layer is then turned over, is applied pair further according to the soil degree divided
The microbial bacterial agent of application amount and soil improvement particle, finally irrigate pollution of chromium soil, and keep relative moisture of the soil
Up to more than 85% 10~20 day.And this method remediation efficiency height, at low cost, non-secondary pollution are pointed out in the literature,
Adaptation to local conditions more precisely can quickly repair Subsidence Land in Coal Mining Area chromium-polluted soil, effectively chromium be prevented to migrate in the soil, and
Nutrient is provided for soil for a long time in soil remediation process, improves the use value of soil.But this method is only
It is to employ single microorganism remediation technology, remediation efficiency by experimental verification, does not lack persuasion.Also, this side
Method is effective only for the chromium in heavy metal pollution of soil object, other Main Heavy Metals, such as copper, lead, mercury, arsenic are still
It cannot so be effectively treated, soil pollution situation can not thoroughly be improved, and also limit the practical application of this method.
Invention content
It is biological prosthetic using two kinds of different types present invention aims at a kind of method for repairing coal mining area soil of offer
The synergistic effect generated between means realizes that single creature repairs the repairing effect that means are unable to reach, while can be directed to more
The different heavy metal of kind carries out bio-absorbable, thoroughly improves soil quality, improves the utility value of coal mining area soil.
The technical solution adopted in the present invention is：A kind of method that plant-microorganism joint repairs coal mining area soil, is pressed
It is carried out according to following steps：
Step 1: according to mass percent, by the Trisetum bifidum seed of water content 12-15% in the stone that mass percent concentration is 1%
It pulls the cotton gauze covering evenly laid out, then use moistens after being impregnated 1-2 hours in buck out, 20-30 DEG C of temperature is kept to pour daily
Water 2-4 times, single irrigation amount is subject to cotton gauze cannot accommodate water again, until Trisetum bifidum germination；
Step 2: by seedling culture hole plate of the Trisetum bifidum seed implantation equipped with seedling medium of germination, 1.5-2cm, each cave are flowed or blow over and cover completely
Disk is implanted into 2-3, keeps 22-32 DEG C of temperature, and seedling medium relative humidity is 65-85%, watering 2-3 times daily, single irrigation amount
Being subject to hole tray bottom starts water seepage, until the seedling strain of Trisetum bifidum drills out ground；
Step 3: after seedling strain drills out ground, control seedling culture hole plate temperature is 25-35 DEG C, and seedling medium relative humidity is 60%-
80%, watering 2-3 times daily, single irrigation amount is subject to seedling culture hole plate bottom and starts water seepage, normal except sunlight is received daytime
Irradiation is outer, and daily 3:30-5:The blue light progress light filling processing that 30 use intensities of illumination are 2200-2300Lx, 21:30-23:30
Light filling processing is carried out using the green light that intensity of illumination is 1800-2000Lx, when seedling strain is long to when exposing 3 centimetres, removal is extra
Seedling strain, retain a young plant strain in each seedling culture hole plate, fill the gaps with seedlings the cavities for not having emergence in seedling culture hole plate, while is artificial
Clear up weeds；
Step 4: after completing step 3, Mixed Microbes dilution is applied in the seedling culture hole plate with seedling strain, applied amount is with nursery
Until hole tray bottom starts to ooze out Mixed Microbes dilution, the configuration process of Mixed Microbes dilution is by acidophilus ferrous oxide sulphur bar
Bacterium, rainbow conk keyhole bacterium, the secondary hat mould solid fungicide of sacculus are according to mass ratio (5-7):(2-3):(1-2) is mixed, and obtains mix bacterium agent,
Then mix bacterium agent is diluted 100 times with distilled water, obtains Mixed Microbes dilution；
Step 5: by the seedling transplant to the contaminated soil in mining area by pretreatment for applying Mixed Microbes dilution, it is raw
Irregularly watering ensures that soil moisture is not less than the 50% of field capacity to growth process, after growth is more than 30 centimetres, prescinds Trisetum bifidum
Stalk, whether detection soil physico-chemical index is up to standard, and if not up to standard, repetition is planted primary or multiple.
As a kind of preferred embodiment：The configuration process of seedling medium is in step 2, by mass ratio by rural area soil 25-35
Part, 9-13 parts of humus, 4-6 parts of charring rice husk, 3-5 parts of chicken manure are uniformly mixed, and are then sprayed carbendazim and are carried out disinfection, carbendazim
Fountain height for every 1 cubic metre sprinkling 5 liters of 500 times of liquid carbendazim.
As a kind of preferred embodiment：Acidithiobacillus ferrooxidans strain GF, rainbow conk keyhole bacterium, the secondary hat mould solid of sacculus in step 4
Microbial inoculum is 5 according to mass ratio:3:1.
As a kind of preferred embodiment：The preprocessing process of the contaminated soil in mining area is in step 5, and mining area is contaminated
Soil carry out it is smooth, then soil surface apply humic acid, thickness be 2-3 centimetres, then by 30 lis below upper soll layer
The thick soil of rice is uniformly mixed with humic acid by turning over, ditching after 20 days, 0.25 meter of ditch depth, 0.6-0.8 meters of line-spacing.
As a kind of preferred embodiment：Migration process in step 5 is to involve the seedling for applying Mixed Microbes dilution together in a criminal case
For attached soil from seedling culture hole plate transplanting in ditch, it is 10-20 centimetres to return native cladding thickness, and planting density is per 800-1200 plants of square meter.
The biological prosthetic involved plant of the present invention, the basic condition of microorganism kind are as follows：
Trisetum bifidum（Trisetumbifidum）Alias crab hook grass, belongs to grass family, and herbaceos perennial is grown on height above sea level 490-
At 2500 meters of hillside roadside, the Lin Yin and wet meadow of limes marginis.Be more common in China Gansu, Tibet, Shaanxi, Henan, Jiangsu, Anhui,
The provinces and regions such as Zhejiang, Fujian, Jiangxi, Hubei, Hunan, Sichuan, Guizhou, Yunnan, Guangxi, Guangdong.Suitable in fertile loam or sand
It is grown on loam, is relatively resistant to acid soil.Early spring sprouts, and growth is very fast, and May blooms, and June is solid, easy to fall off after seed maturation,
Main row seminal propagation.
Acidithiobacillus ferrooxidans strain GF（Acidithiobacillusferrooxins）Preferably energy of oxidation autotrophic microbe,
With the CO in air2For carbon source, by the Fe in oxidation matrix2+For Fe3+With inorganic sulfur energy is obtained for sulfate radical.This characteristic
Make it that there is potential application value in fields such as bacterial metallurgy, acid waste water processing, flue gas desulfurization and coal desulfurizations.
Rainbow conk keyhole bacterium（Trametes versicolor）Belong to Basidiomycota wood-rotting fungi, also known as Coriolus Versicolor, color suede leather
Lid bacterium, Coriolus Versicolor, rainbow conk mushroom etc..Research find its containing steroid, triterpenes, organic acid, alkaloids, glucose alcohols,
The compounds such as protein-based, carbohydrate, glycopeptide class.The bacterium is chiefly used in health products, only in wastewater treatment and improvement in field of environment protection
It is applied in terms of soil nickel contamination.
Pair hat sacculus is mould（Glomus coronatum）Belonging to one kind of arbuscular mycorrhizal fungi, spore list is born in soil,
Spherical or hemispherical or irregular shape, 80~150 × 95~230um.It is orange to yellowish-brown.Two layers of conidial cell wall, outer wall are transparent
Wall, thickness are 1.5 ~ 3.5um, this layer of wall is easily ripe with spore and comes off therefore is not easy to observe；Yellow to brown inner wall thickness
Up to 4~6um, even point is roomy for mycelia, and up to 30~42um, typical funnel-form connects spore mycelia and spore junction Chang Youyi
A bending every separate, possess some special knowledge and apply in terms of as plant bacterial manure at present.
The mechanism of action that the present invention repairs coal mining area soil is as follows：
Plant-microorganism combined remediation technology is the compound system that is formed using plant and microorganism come common degradation of contaminant,
Remove a kind of pollution treatment technology of environmental contaminants.The method using soil, microorganism, plant Coexistence, fully send out
Plant and microorganism remediation technology respectively advantage are waved, is covered the shortage, and then improves the phytoremediation efficiency of pollutant in soil, most
Achieve the purpose that thoroughly to repair soil eventually.Microorganism is combined to form homobium with plant, such as mycorhiza, it is possible to reduce plant roots
It is the absorption to heavy metal, promotes the rapid field planting of plant, therefore very necessary in ecology of mining areas reparation.
Arbuscular mycorrhizal fungi, can be with land as a kind of beneficial symbiotic microorganism for being prevalent in terrestrial plant rhizosphere
Upper 80% plant forms homobium, mineral can be supported to improve plant by its huge mycelia network under obigotrophic condition
The absorbability divided；Extreme environment, by nutrition improvement enhance the preventing from heavy metal of plant, drought-resistant ability, antibacterial ability with
And carbon sequestration capacity, finally significantly improve the microhabitat of soil.
Trisetum bifidum is stronger for the adaptability of growing environment, is resistant to acid soil environment, when being colonized in coal mining area
After soil, root system can absorb the various heavy in soil, such as copper, zinc, lead, chromium, mercury, and growth period is only 3 months
Left and right, can in the short term repeated growth, harvesting, improve soil remediation efficiency.Secondary hat sacculus in mix bacterium agent is mould not only
Homobium can be formed with plant, improve the ability of plant absorption heavy metal, it can also be with Acidithiobacillus ferrooxidans strain GF and cloud
Stable micro-ecological environment is collectively formed in sesame keyhole bacterium, decomposes the organic pollution in soil.What is more important, using three
The composite bacteria agent of composition, can be than only further significantly improving Trisetum bifidum for heavy metal using single arbuscular mycorrhizal fungi
Absorption efficiency and tolerance improve the repairing effect of coal mining area contaminated soil so that this technology has practical popularization
The feasibility of application.
Beneficial effects of the present invention are：
（1）The present invention to shorter herbaceous plant of a variety of growth periods by carrying out field trial, it is determined that Trisetum bifidum is as reparation
Plant, and realize expected repairing effect.Trisetum bifidum is merely possible to herding forage grass in research before a little answer
With, and the reparation for being applied to heavy metal soil still belongs to the first time.
（2）The present invention is final to determine by the way that a variety of microorganisms for being applied to soil remediation are screened and matched
Acidithiobacillus ferrooxidans strain GF, rainbow conk keyhole bacterium, secondary hat sacculus this mould combination.Three kinds of bacterium are in the inventive solutions
Play the role of differentiation, and cooperate, more there is synergy compared with using single culture, it is above-mentioned micro-
Biology can not only effectively facilitate the field planting of Trisetum bifidum and improve the ability that Trisetum bifidum root system absorbs heavy metal ion, can also live
Change the heavy metal in soil, improve biological effectiveness.
（3）The present invention recycles seedling culture hole plate to cultivate seedling, finally buries seedling particular by grass seeds germination is first promoted
The field planting of Trisetum bifidum is realized in the mode of mining soil growth, this mode directly sows grass-seed in the soil compared with common,
The survival rate of Trisetum bifidum can be greatly improved.And in nursery by dividing at times after optimizing the proportioning of seedling medium, emerging
Wavelength carries out light filling processing, promotes the growth and development of plant seedlings, and improves the seedling after the field planting of coal mining area soil
Tolerance, upgrowth situation, which has, after Seeding planting significantly improves.
（4）The present invention applies humic acid to coal mining area soil and reacts, so as to improve the physicochemical property of soil, profit
In the field planting and growth of Trisetum bifidum seedling.Rational planting density and field management have also for the maximization of final repairing effect
The influence that can not ignore.
Specific embodiment
The present invention is described in further detail with reference to embodiment.
Coal mining area soil remediation is carried out using Trisetum bifidum and mix bacterium agent：
（1）Under conditions of climate temperature is higher than 10 DEG C, Trisetum bifidum seed is laid in the ground covered with plastic film, is utilized
Sunlight is exposed to the sun, and the cumulative time of being exposed to the sun is 24 hours, is then soaked seed using a concentration of 1% limewash, is dragged for after impregnating 2 hours
Go out grass seeds, covered using the cotton gauze of wetting on sandy soil by grass seeds is evenly laid out, keep 25 DEG C of temperature, water 3 daily
Secondary, single irrigation amount, which is subject to, soaks cotton gauze, until grass seeds germinates；
（2）Proportioning according to 25 parts of rural area soil, 13 parts of humus, 5 parts of charring rice husk, 4 parts of chicken manure is configured to seedling medium, then
Sprinkling carbendazim carries out disinfection, and fountain height sprays the carbendazim of 500 times of liquid of 5L for every 1 cubic metre of seedling medium, then by nursery
Matrix is contained in seedling culture hole plate, then the grass seeds after germination is seeded in seedling culture hole plate, and control nursery temperature is 28 DEG C, matrix
Relative humidity is 80%, daily watering 3 times, and single irrigation amount is advisable with hole tray bottom exudation little water, until emergence；
（3）After emergence, control nursery temperature is 28 DEG C, matrix relative humidity is 75%, daily watering 3 times, and single irrigation amount is with cave
Tray bottom exudation little water is advisable, and daily 3:30-5:30 are carried out using the blue light that intensity of illumination is 2200Lx at light filling
Reason, 21:30-23:30 carry out light filling processing using the green light that intensity of illumination is 2000 Lx, when seedling strain is long to up to 3 centimetres
When, seedling extra in cavities is removed, ensures that single cavities only retains one plant of seedling, and to not having emergence in seedling culture hole plate
Cavities is filled the gaps with seedlings, while manual cleaning weeds；
（4）The mould source of acid oxidase ferrous iron Thiobacillus, rainbow conk keyhole bacterium, secondary hat sacculus is as follows：Acidithiobacillus ferrooxidans strain GF is purchased
It buys in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number is CGMCC No.3265；Rainbow conk bolt
Pore fungi is bought in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number CGMCC
No.12241；For the mould purchase of pair hat sacculus in Arbuscular Mycorrhizal Fungi In China germplasm resource bank, deposit number is BGC NM06A.
In use, the previously prepared secondary hat mould solid fungicide of sacculus, using the river sand of sieving as matrix, steams through high pressure at 121 DEG C
It after vapour sterilizing, places 8 days under natural conditions, then fills 2 Kg and be put into basin, the inoculation 100g pair hat mould original seeds of sacculus add after mixing thoroughly
Enter the tap water of matrix weight 25%, sow sand sagebrush (Artemisia filifolia) seed, keep normal growth, can obtain after five to six months and ball is preced with by pair
The host plant that capsule mould is infected and the matrix containing mycelia outside secondary hat sacculus mycotic spore, root；Sand sagebrush (Artemisia filifolia) aerial part is cut off, and is cut
Broken root segment, and with the matrix mixing containing mycelia outside secondary hat sacculus mycotic spore, root, obtain the secondary hat mould solid fungicide of sacculus.
Acid oxidase ferrous iron Thiobacillus, rainbow conk keyhole bacterium are respectively connected in different shaking flask fluid nutrient mediums, shaken in shaking flask
Constant temperature incubation in device is swung, collects seed liquor, is then separately added into the small-sized fermentation tank equipped with different culture media and continues to expand training
It supports, the quantity of viable count is made to reach 1 × 108A/ml.Culture medium used in acid oxidase ferrous iron Thiobacillus includes per 1L：
FeSO4·7H2O44.7g, (NH4)2SO43.0g, K2HPO40.5g, MgSO4·7H2O0.5g, KCl0.1g, Ca (NO3) 20.01g,
The pH value of culture medium is 2.Culture medium used in rainbow conk keyhole bacterium includes per 1L：KH2PO41.0g, Na2HPO40.2g,
MgSO4·7H2O 0.5g, VB1 0.1mg, CaCl20.1mg, FeSO4·7H2O 0.1mg, ZnSO4·7H2O 0.01mg,
CuSO4·5H2O 0.2mg, glucose 1.0g, ammonium tartrate 0.1g, the pH value of culture medium is 5.
By Acidithiobacillus ferrooxidans strain GF, the isolated thalline of rainbow conk keyhole bacterium after expanding and cultivating, obtained with prior
The secondary hat mould solid fungicide of sacculus according to mass ratio with Acidithiobacillus ferrooxidans strain GF：Rainbow conk keyhole bacterium：The pair hat mould solid of sacculus
Microbial inoculum=5:3:1 mixing, obtains mix bacterium agent, is then diluted 100 times with distilled water, obtain Mixed Microbes dilution, will mix
Bacterium dilution is applied in each cavities with seedling, and single applied amount oozes out a little dilution with hole tray bottom to be advisable；
（5）Certain state-owned coal mines positioned at Shanxi Province Xinzhou City Ningwu County are selected, delineation trial stretch is coal mining region
Waste land in 5 kilometers of center radius, the contaminated soil progress in mining area is smooth, then apply humic acid in soil surface
（Humic acid original powder is bought from Shanxi Jin Wo humic acid Science and Technology Ltd., and active constituent content is：Humic acid >=50%, organic matter
>=80%, moisture content≤25% of moisture content≤20%）Thickness is 2.5 centimetres, then by the soil of 30 cm thicks below upper soll layer and corruption
It is uniformly mixed by turning over to grow acid, reacts 20 days, then according to 0.7 meter of line-spacing ditching, 0.25 meter of ditch depth will apply mixing
The Trisetum bifidum seedling of bacterium dilution is taken out together with attached soil from the cavities of seedling culture hole plate, is embedded in ditch, is then returned soil and is covered seedling
Lid, it is 15 centimetres to return native cladding thickness, and planting density is per 1000 plants of square meter；
（6）Field management is carried out to the Trisetum bifidum of plantation, irregularly watering ensures soil moisture according to soil moisture actual conditions
Not less than the 50% of field capacity, Trisetum bifidum is gathered in after growth 100 days.
Meanwhile the contaminatedground in delineation trial stretch is repaired according to identical experimental condition plantation Trisetum bifidum,
It differs only in step（4）The middle single secondary hat mould replacement mix bacterium agent of sacculus, other be related to strain expand culture and
The parameters for applying microbial inoculum are constant, and reference examples are repaired as plant and single microbial inoculum.
Heavy metal content in soil variation measures and the harvested heavy metal adsorption of Trisetum bifidum measures：
Content of beary metal in soil measures with the following method：Remove the sundries such as rubble, the plant undesirable root in pedotheque, wind
It is sieved with 100 mesh sieve after dry spare.Using HNO3:HF:HClO4(v:v=4:4:2) mix acid liquor resolution, with general analysis TAS-900 atoms
Absorption spectrophotometer measures the content of copper, zinc, lead, chromium, mercury, arsenic.
Content of beary metal after harvesting in Trisetum bifidum plant measures with the following method：By Trisetum bifidum plant together with root system
Finish 30 min at 105 DEG C, with drying to constant weight and crushes after 75 DEG C, sieves with 100 mesh sieve.Then use HClO4:HNO3（v:v=
2:5）Mix acid liquor resolution, measure copper, zinc, lead, chromium, mercury, arsenic with general analysis TAS-900 atomic absorption spectrophotometers and contain
Amount.
Using said determination method, measure respectively original in certain state-owned coal mines trial stretch of Shanxi Province Xinzhou City Ningwu County
Heavy metal copper in soil, zinc, lead, chromium, mercury, arsenic content（Sample 1）, through Trisetum bifidum repair a cycle after soil in weight
Metallic copper, zinc, lead, chromium, mercury, arsenic content（Sample 2）, heavy metal in the Trisetum bifidum gathered in after the completion of a repairing efficiency
Copper, zinc, lead, chromium, mercury, arsenic content（Sample 3）And the plant gathered in after the completion of a repairing efficiency is repaiied with single microbial inoculum
The content of heavy metal copper, zinc, lead, chromium, mercury, arsenic in multiple reference examples Trisetum bifidum（Sample 4）, every group of sample do three it is parallel, it is every
Test index is shown in Table 1-4.
The content of heavy metal copper, zinc, lead, chromium, mercury, arsenic in 1 initial soil of table（mg/Kg）
Table 2 through Trisetum bifidum repair a cycle after heavy metal copper in soil, zinc, lead, chromium, mercury, arsenic content（mg/Kg）
The content of heavy metal copper, zinc, lead, chromium, mercury, arsenic in the Trisetum bifidum gathered in after the completion of 3 one repairing efficiencies of table（mg/Kg）
The plant gathered in after the completion of 4 one repairing efficiencies of table and single microbial inoculum repair heavy metal copper in reference examples Trisetum bifidum,
Zinc, lead, chromium, mercury, arsenic content（mg/Kg）
Trisetum bifidum is can be seen that as coal mining area soil repairing plant from above-mentioned experimental result, it can be in a growth cycle
It inside allows in soil more than copper, zinc, lead, chromium, mercury, the arsenic these types content of beary metal of I grades of standards of national soil environment quality
Index is remarkably decreased, other several contents of heavy metal elements in addition to lead be fully lowered into standard maximum limit value it
Under, obtain good repairing effect.Also, it for the reference examples for only applying the secondary hat mould dilution of sacculus, is applied with mixed
The Trisetum bifidum for closing bacterium dilution is equal for copper, zinc, lead, chromium, mercury, the absorbability of arsenic these types heavy metal within growth period
It is significantly increased, Mixed Microbes dilution plays considerable effect for the Metal uptake ability of Trisetum bifidum, so as to make
Obtain the prospect that this geobiont repair method more has promotion and implementation.
Although the present invention is made that by generality explanation, specific embodiment and experiment in embodiment detailed
Description, but on the basis of without departing from core of the present invention, the modification or improvement that can still make belong to that the present invention claims guarantors
The range of shield.
Claims (5)
1. a kind of method that plant-microorganism joint repairs coal mining area soil, it is characterised in that：It carries out in accordance with the following steps：
Step 1: according to mass percent, by the Trisetum bifidum seed of water content 12-15% in the stone that mass percent concentration is 1%
It pulls the cotton gauze covering evenly laid out, then use moistens after being impregnated 1-2 hours in buck out, 20-30 DEG C of temperature is kept to pour daily
Water 2-4 times, single irrigation amount is subject to cotton gauze cannot accommodate water again, until Trisetum bifidum germination；
Step 2: by seedling culture hole plate of the Trisetum bifidum seed implantation equipped with seedling medium of germination, 1.5-2cm, each cave are flowed or blow over and cover completely
Disk is implanted into 2-3, keeps 22-32 DEG C of temperature, and seedling medium relative humidity is 65-85%, watering 2-3 times daily, single irrigation amount
Being subject to hole tray bottom starts water seepage, until the seedling strain of Trisetum bifidum drills out ground；
Step 3: after seedling strain drills out ground, control seedling culture hole plate temperature is 25-35 DEG C, and seedling medium relative humidity is 60%-
80%, watering 2-3 times daily, single irrigation amount is subject to seedling culture hole plate bottom and starts water seepage, normal except sunlight is received daytime
Irradiation is outer, and daily 3:30-5:The blue light progress light filling processing that 30 use intensities of illumination are 2200-2300Lx, 21:30-23:30
Light filling processing is carried out using the green light that intensity of illumination is 1800-2000Lx, when seedling strain is long to when exposing 3 centimetres, removal is extra
Seedling strain, retain a young plant strain in each seedling culture hole plate, fill the gaps with seedlings the cavities for not having emergence in seedling culture hole plate, while is artificial
Clear up weeds；
Step 4: after completing step 3, Mixed Microbes dilution is applied in the seedling culture hole plate with seedling strain, applied amount is with nursery
Until hole tray bottom starts to ooze out Mixed Microbes dilution, the configuration process of Mixed Microbes dilution is by acidophilus ferrous oxide sulphur bar
Bacterium, rainbow conk keyhole bacterium, the secondary hat mould solid fungicide of sacculus are according to mass ratio (5-7):(2-3):(1-2) is mixed, and obtains mix bacterium agent,
Then mix bacterium agent is diluted 100 times with distilled water, obtains Mixed Microbes dilution；
Step 5: by the seedling transplant to the contaminated soil in mining area by pretreatment for applying Mixed Microbes dilution, it is raw
Irregularly watering ensures that soil moisture is not less than the 50% of field capacity to growth process, after growth is more than 30 centimetres, prescinds Trisetum bifidum
Stalk, whether detection soil physico-chemical index is up to standard, and if not up to standard, repetition is planted primary or multiple.
2. the method that a kind of plant-microorganism joint according to claim 1 repairs coal mining area soil, feature exist
In：The configuration process of seedling medium is in step 2, by mass ratio by 25-35 parts of rural area soil, 9-13 parts of humus, charring rice husk
4-6 parts, 3-5 parts of chicken manure be uniformly mixed, then spray carbendazim and carry out disinfection, the fountain height of carbendazim is every 1 cubic metre sprinkling 5
Rise the carbendazim of 500 times of liquid.
3. the method that a kind of plant-microorganism joint according to claim 1 repairs coal mining area soil, feature exist
In：Acidithiobacillus ferrooxidans strain GF, rainbow conk keyhole bacterium, the secondary hat mould solid fungicide of sacculus are 5 according to mass ratio in step 4:3:1.
4. the method that a kind of plant-microorganism joint according to claim 1 repairs coal mining area soil, feature exist
In：The preprocessing process of the contaminated soil in mining area is in step 5, and the contaminated soil progress in mining area is smooth, then in soil
Earth surface apply humic acid, thickness be 2-3 centimetres, then by the soil of 30 cm thicks below upper soll layer with humic acid by turning over
It ploughs and is uniformly mixed, ditching after 20 days, 0.25 meter of ditch depth, 0.6-0.8 meters of line-spacing.
5. the method that a kind of plant-microorganism joint according to claim 4 repairs coal mining area soil, feature exist
In：Migration process in step 5 is to involve the seedling for applying Mixed Microbes dilution in a criminal case and transplanted with attached soil from seedling culture hole plate in ditch
In, it is 10-20 centimetres to return native cladding thickness, and planting density is per 800-1200 plants of square meter.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109168391A (en) * | 2018-09-20 | 2019-01-11 | 鞍钢集团矿业有限公司 | A kind of method of iron tailings soil |
| CN109197783A (en) * | 2018-09-25 | 2019-01-15 | 山西大学 | A kind of production method of pure natural ceromel |
| CN112090954A (en) * | 2020-08-31 | 2020-12-18 | 山西大学 | Activated and degraded coagulated beads, preparation method thereof and degradation method of polycyclic aromatic hydrocarbon-polluted soil |
| CN112588800A (en) * | 2020-12-01 | 2021-04-02 | 天津市滨海新区环境创新研究院 | Remediation of ionic rare earth waste tailing area soil by using AM fungi and hybrid pennisetum alopecuroides |
| CN115443879A (en) * | 2022-10-13 | 2022-12-09 | 中国矿业大学 | Preparation method of matrix coal gangue and seedling raising matrix based on coal gangue |
| CN117408499A (en) * | 2023-12-15 | 2024-01-16 | 国家林业和草原局产业发展规划院 | Method and system for supervising remote sensing information of forest and grass resource monitoring |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102577826A (en) * | 2012-03-19 | 2012-07-18 | 河南科技大学 | Method for using arbuscular mycorrhizal fungi and organic fertilizer to reduce residue of lead and cadmium in tobacco |
| CN103350105A (en) * | 2013-06-26 | 2013-10-16 | 深圳清华大学研究院 | Method for associating plants and microorganisms to accumulate heavy metal cadmium in soil and application thereof |
| CN105312310A (en) * | 2015-02-04 | 2016-02-10 | 四川农业大学 | Method for bioremediation of heavy metal contaminated soil |
| KR20160092536A (en) * | 2015-01-27 | 2016-08-05 | 화이젠 주식회사 | Growth Inhancing Agent for Microorganism and The Method of Phytoremediation on Soil Contaminated with Heavy Metal Using The Same |
| CN106433673A (en) * | 2016-09-13 | 2017-02-22 | 山西潞安集团司马煤业有限公司 | Preparation method and use method of soil conditioner for coal mining area |
| CN106607454A (en) * | 2016-12-20 | 2017-05-03 | 平顶山学院 | Remediation method for chrome-polluted soil in coal mine collapse region |
-
2018
- 2018-01-02 CN CN201810000526.8A patent/CN108160685B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102577826A (en) * | 2012-03-19 | 2012-07-18 | 河南科技大学 | Method for using arbuscular mycorrhizal fungi and organic fertilizer to reduce residue of lead and cadmium in tobacco |
| CN103350105A (en) * | 2013-06-26 | 2013-10-16 | 深圳清华大学研究院 | Method for associating plants and microorganisms to accumulate heavy metal cadmium in soil and application thereof |
| KR20160092536A (en) * | 2015-01-27 | 2016-08-05 | 화이젠 주식회사 | Growth Inhancing Agent for Microorganism and The Method of Phytoremediation on Soil Contaminated with Heavy Metal Using The Same |
| CN105312310A (en) * | 2015-02-04 | 2016-02-10 | 四川农业大学 | Method for bioremediation of heavy metal contaminated soil |
| CN106433673A (en) * | 2016-09-13 | 2017-02-22 | 山西潞安集团司马煤业有限公司 | Preparation method and use method of soil conditioner for coal mining area |
| CN106607454A (en) * | 2016-12-20 | 2017-05-03 | 平顶山学院 | Remediation method for chrome-polluted soil in coal mine collapse region |
Non-Patent Citations (3)
| Title |
|---|
| 任义芳，贾明强，刘润进: "《菌根真菌与其他生物联合修复污染与退化土壤的效应与机制》", 《青岛农业大学学报（自然科学版）》 * |
| 孙金华等: "《接种ＡＭ 菌对西部黄土区采煤沉陷地柠条生长和土壤的修复效应》", 《生态学报》 * |
| 黄占斌，孙朋成: "《矿区重金属污染土壤的修复》", 《科学》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109168391A (en) * | 2018-09-20 | 2019-01-11 | 鞍钢集团矿业有限公司 | A kind of method of iron tailings soil |
| CN109197783A (en) * | 2018-09-25 | 2019-01-15 | 山西大学 | A kind of production method of pure natural ceromel |
| CN112090954A (en) * | 2020-08-31 | 2020-12-18 | 山西大学 | Activated and degraded coagulated beads, preparation method thereof and degradation method of polycyclic aromatic hydrocarbon-polluted soil |
| CN112588800A (en) * | 2020-12-01 | 2021-04-02 | 天津市滨海新区环境创新研究院 | Remediation of ionic rare earth waste tailing area soil by using AM fungi and hybrid pennisetum alopecuroides |
| CN115443879A (en) * | 2022-10-13 | 2022-12-09 | 中国矿业大学 | Preparation method of matrix coal gangue and seedling raising matrix based on coal gangue |
| CN117408499A (en) * | 2023-12-15 | 2024-01-16 | 国家林业和草原局产业发展规划院 | Method and system for supervising remote sensing information of forest and grass resource monitoring |
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