CN102577826A - Method for using arbuscular mycorrhizal fungi and organic fertilizer to reduce residue of lead and cadmium in tobacco - Google Patents
Method for using arbuscular mycorrhizal fungi and organic fertilizer to reduce residue of lead and cadmium in tobacco Download PDFInfo
- Publication number
- CN102577826A CN102577826A CN201210071205XA CN201210071205A CN102577826A CN 102577826 A CN102577826 A CN 102577826A CN 201210071205X A CN201210071205X A CN 201210071205XA CN 201210071205 A CN201210071205 A CN 201210071205A CN 102577826 A CN102577826 A CN 102577826A
- Authority
- CN
- China
- Prior art keywords
- tobacco
- amf
- cadmium
- fertilizer
- microbial inoculum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
A method for using arbuscular mycorrhizal fungi and organic fertilizer to reduce residue of lead and cadmium in tobacco includes: preparing arbuscular mycorrhizal fungi inoculant, culturing mycorrhizal tobacco seedlings, and transplanting and managing the mycorrhizal seedlings. Tests show that arbuscular mycorrhizal fungi and cow dung added to soil coordinate to promote plant growth, increase phosphorus content of vegetation, lower poison and pollution of tobacco by lead and cadmium and promote tobacco growth, thereby reducing the residue of lead and cadmium in the tobacco.
Description
Technical field
The present invention relates to a kind of tobacco growing, plumbous, residual method of cadmium of reduction tobacco of promoting, a kind of specifically AMF and fertilizer of utilizing reduces tobacco lead, the residual method of cadmium.
Background technology
Tobacco is the important economic crops of China; Tobacco is again a kind of Special food simultaneously; China's smoking is populous, so its quality safety extensively paid attention to, in recent years; The report of quality security problems such as tobacco heavy metals exceeding standard is often arranged, and this certainly will have influence on the competitiveness and the sound development of China's tobacco industry.China, the cultivated area of tobacco, yield of flue-cured tobacco and output all occupy first place in the world, and the heavy metal in the tobacco can get into human body through burning with main flume in aspiration procedure, human body is caused potential hazard.Increasing research confirms; Smoking has become one of some main sources of heavy metal of smoker; The too high soil of being grown with tobacco of heavy-metal residual has certain relation in the tobacco, and the content of beary metal of tobacco that also can cause lack of standardization of fertilizer and agricultural chemicals is too high simultaneously, and wherein cadmium content is the highest.
At present; For the method that reduces heavy-metal residual in the tobacco; Except reducing the tobacco planting environmental pollution; Inorganic amendments such as lime commonly used, fused calcium magnesium phosphate, clay mineral are used for reducing the tobacco heavy-metal residual, and Chinese invention patent (CN101724405A) has been invented a kind of soil conditioner that reduces crops cadmium, lead pollution, adopts Ca (OH)
2, Na
2SiO
7Be used for reducing the problem of heavy-metal residual in the tobacco Deng inorganic component, but existing in complex process, increased production cost, can not better application in tobacco industry.
Summary of the invention
The present invention is directed to the deficiency of the problems referred to above, provide and utilize AMF and fertilizer to reduce that tobacco is plumbous, the residual method of cadmium, its technology is simple, and is easy to operate, effectively reduces the residual of cadmium in the tobacco, reduces production costs.
The present invention addresses the above problem the technical scheme that is adopted to be: utilize AMF and fertilizer to reduce tobacco lead, the residual method of cadmium, may further comprise the steps:
The preparation of step 1, AMF microbial inoculum
1) to get 3 parts of particle diameters by volume be 1mm river sand and 1 part of peat that particle diameter is 1mm mix, and form mixture, after with mixture under 121 ℃ of conditions, sterilize 50-60 minutes, taking-up is cooled to 30-40 ℃, makes mixed-matrix, and is subsequent use;
2) in the ratio that adds 7.8g urea and 33g superphosphate in every 100kg mixed-matrix, add urea and superphosphate in the mixed-matrix in step 1) after the sterilization, mix, form base fertilizer, subsequent use;
3) get 95-97% base fertilizer and 3-5% initial AMF microbial inoculum by weight percentage, mix, form culture mix; With culture mix weight is benchmark, in culture mix, adds 15-25% water, back sowing clover grass seed; By the conventional cultural method of clover grass, cultivated 3-4 months, obtain clover grass that is infected by AMF and the matrix that contains the outer mycelia of AMF spore and root; The root of clover grass is cut into the segment of 0.5-1mm; Mix with the matrix that contains the outer mycelia of AMF spore and root then, make the AMF microbial inoculum, subsequent use;
The cultivation of step 2, Mycorrhizal cigarette seedling
In the seedling-cultivating tray of floatation of foam hole, add the AMF microbial inoculum that makes in the step 1, sowing tobacco seed in back immerses in the water 1/3 place of seedling-cultivating tray bottom; Treat thinning behind the seed sprouting, make and stay 1 tobacco seedling in every hole, and change water into low-phosphorous nutrient solution; Supply the moisture that reduces in the low-phosphorous nutrient solution every day, per 2-3 weeks are changed once low-phosphorous nutrient solution, grow to 50-60 days after; Get infection rate greater than 30% Mycorrhizal cigarette seedling, subsequent use;
The transplanting and the management of step 3, Mycorrhizal cigarette seedling
In field soil, dig and transplant the cave, the Mycorrhizal cigarette seed that obtains in the step 2 is implanted in transplants in the cave, adopt the method for spreading fertilizer over the fields to apply fertilizer then in the field; Applied amount adds by 15000-22500 kg/ha; Mycorrhizal cigarette seedling is cultivated, added chemical fertilizer in the nurturing period, the addition of chemical fertilizer applies by the convention amount of tobacco planting; Carry out field management such as moisture, weeds simultaneously, make the tobacco normal growth to results;
Described fertilizer is the cow dung of becoming thoroughly decomposed;
The bacterial classification of described AMF microbial inoculum is that Scotland sacculus is mould
Glomus caledonium90036, it is mould that nectarine does not have the stalk capsule
Acaulospora melleaZZ, Glomus mosseae
Glomus mosseaeSacculus is mould in BEG167 or the root
Glomus intraradicesAmong the BEG141 any one.
The constituent of described low-phosphorous nutrient solution: the KH that contains 11.3 mg in every liter of low-phosphorous nutrient solution
2PO
4, 126.3 mg KNO
3, 295 mg Ca (NO
3)
2, 123.3 mg MgSO
4, 0.715 mg H
3BO
3, 0.453 mg MnCl
24H
2The H of O, 0.005 mg
2MoO
4H
2The ZnSO of O, 0.055 mg
47H
2The CuSO of O and 0.02 mg
45H
2O wherein also adds in low-phosphorous nutrient solution by 1.863 mg EDTA and 1.393 mg FeSO
47H
2The chelating attitude Fe-EDTA that O constitutes.
Used tobacco seed in technique scheme is general commercially available prod, and used among the present invention is tobacco K326, buys cigarette seed Co., Ltd in Qingdao.
Used AMF bacterial classification in technique scheme,, nectarine mould for Scotland sacculus do not have that the stalk capsule is mould, sacculus any one in mould in Glomus mosseae or the root, all can on market, buy to obtain.
Beneficial effect
1, in the present invention, plumbous, cadmium is two stronger heavy metal elements of phytotoxicity, can suppress root system development and to nutrient absorbing, thus the growth of the whole strain plant of influence.Add AMF and cow dung among the present invention, can significantly reduce the murder by poisoning of lead, cadmium, promote tobacco growing.
2, apply cow dung and AMF in the present invention, collaborative promote plant growing, improve phosphorus content in the vegetation jointly, reduce murder by poisoning and the pollution of plumbous, cadmium tobacco.Cow dung is except supplying with the nutrient to soil; Also increase the shock-absorbing capacity and the adsorption capacity of soil; And by effects such as absorption, complexing, reduction, volatilizations; Reduce its biological effectiveness and plant poisoning; Promote the plant growth; The phytoremediation that helps heavy-metal contaminated soil supplies the plant absorbing utilization for tobacco provides the phosphorus source; AMF can pass through the mobility that chelation, the effect of mycelia fixing etc. suppress heavy metal; By improve the plant nutrient situation, change the rhizosphere physicochemical character, change Root morphology, the number of ways such as synthetic of regulating some expression of gene and heavy metal inducible protein improves plant patience; But use separately; Effect is relatively poor; Among the present invention; Add cow dung and AMF simultaneously; Can better impel organic matter and plumbous, cadmium that various physical and chemical effects take place, reduce its biological effectiveness and phytotoxicity.
3, tentative confirmation of the present invention the inoculation AMF and apply cow dung can improve yield of tobacco, reduce plumbous, cadmium is residual, the two has synergy, this production for nuisanceless tobacco leaf in the heavy-metal contaminated soil is significant.Simultaneously, tobacco has stronger absorption, accumulation capability as the high-biomass crop to cadmium, lead, copper, zinc, nickel, mercury heavy metal, also can be applied to the phytoremediation of heavy-metal contaminated soil.
4, promotion tobacco growing provided by the invention, reduce tobacco heavy metal lead, the residual method of cadmium, its technology is simple, and is simple to operate, can effectively reduce the residual of plumbous in the tobacco, cadmium, improves tobacco quality safety, reduces health risk.
Embodiment
Utilize AMF and fertilizer to reduce tobacco lead, the residual method of cadmium, may further comprise the steps:
The preparation of step 1, AMF microbial inoculum
1) to get 3 parts of particle diameters by volume be 1mm river sand and 1 part of peat that particle diameter is 1mm mix, and form mixture, after with mixture under 121 ℃ of conditions, sterilize 50-60 minutes, taking-up is cooled to 30-40 ℃, makes mixed-matrix, and is subsequent use;
2) in the ratio that adds 7.8g urea and 33g superphosphate in every 100kg mixed-matrix, add urea and superphosphate in the mixed-matrix in step 1) after the sterilization, mix, form base fertilizer, subsequent use;
3) get 95-97% base fertilizer and 3-5% initial AMF microbial inoculum by weight percentage, mix, form culture mix; With culture mix weight is benchmark, in culture mix, adds 15-25% water, back sowing clover grass seed; By the conventional cultural method of clover grass, cultivated 3-4 months, obtain clover grass that is infected by AMF and the matrix that contains the outer mycelia of AMF spore and root; The root of clover grass is cut into the segment of 0.5-1mm; Mix with the matrix that contains the outer mycelia of AMF spore and root then, make the AMF microbial inoculum, subsequent use;
The cultivation of step 2, Mycorrhizal cigarette seedling
In the seedling-cultivating tray of floatation of foam hole, add the AMF microbial inoculum that makes in the step 1, sowing tobacco seed in back immerses in the water 1/3 place of seedling-cultivating tray bottom; Treat thinning behind the seed sprouting, make and stay 1 tobacco seedling in every hole, and change water into low-phosphorous nutrient solution; Supply the moisture that reduces in the low-phosphorous nutrient solution every day, per 2-3 weeks are changed once low-phosphorous nutrient solution, grow to 50-60 days after; Get infection rate greater than 30% Mycorrhizal cigarette seedling, subsequent use;
The transplanting and the management of step 3, Mycorrhizal cigarette seedling
In field soil, dig and transplant the cave, the Mycorrhizal cigarette seed that obtains in the step 2 is implanted in transplants in the cave, adopt the method for spreading fertilizer over the fields to apply fertilizer then in the field; Applied amount adds by 15000-22500 kg/ha; Mycorrhizal cigarette seedling is cultivated, added chemical fertilizer in the nurturing period, the addition of chemical fertilizer applies by the convention amount of tobacco planting; Carry out field management such as moisture, weeds simultaneously, make the tobacco normal growth to results;
Described fertilizer is the cow dung of becoming thoroughly decomposed;
The bacterial classification of described AMF microbial inoculum is that Scotland sacculus is mould
Glomus caledonium90036, it is mould that nectarine does not have the stalk capsule
Acaulospora melleaZZ, Glomus mosseae
Glomus mosseaeSacculus is mould in BEG167 or the root
Glomus intraradicesAmong the BEG141 any one;
The constituent of described low-phosphorous nutrient solution: the KH that contains 11.3 mg in every liter of low-phosphorous nutrient solution
2PO
4, 126.3 mg KNO
3, 295 mg Ca (NO
3)
2, 123.3 mg MgSO
4, 0.715 mg H
3BO
3, 0.453 mg MnCl
24H
2The H of O, 0.005 mg
2MoO
4H
2The ZnSO of O, 0.055 mg
47H
2The CuSO of O and 0.02 mg
45H
2O wherein also adds in low-phosphorous nutrient solution by 1.863 mg EDTA and 1.393 mg FeSO
47H
2The chelating attitude Fe-EDTA that O constitutes;
Embodiment one
Utilize AMF and fertilizer to reduce the plumbous residual method of tobacco, may further comprise the steps:
The preparation of step 1, AMF microbial inoculum
1) to get 3 parts of particle diameters by volume be 1mm river sand and 1 part of peat that particle diameter is 1mm mix, and form mixture, after with mixture under 121 ℃ of conditions, sterilize 50 minutes, taking-up is cooled to 30 ℃, makes mixed-matrix, and is subsequent use;
2) in the ratio that adds 7.8g urea and 33g superphosphate in every 100kg mixed-matrix, add urea and superphosphate in the mixed-matrix in step 1) after the sterilization, mix, form base fertilizer, subsequent use;
3) get 95-97% base fertilizer and 3-5% initial AMF microbial inoculum by weight percentage, mix, form culture mix; With culture mix weight is benchmark, in culture mix, adds 15-25% water, back sowing clover grass seed; By the conventional cultural method of clover grass, cultivated 3-4 months, obtain clover grass that is infected by AMF and the matrix that contains the outer mycelia of AMF spore and root; The root of clover grass is cut into the segment of 0.5-1mm; Mix with the matrix that contains the outer mycelia of AMF spore and root then, make the AMF microbial inoculum, subsequent use;
The cultivation of step 2, Mycorrhizal cigarette seedling
In the seedling-cultivating tray of floatation of foam hole, add the AMF microbial inoculum that makes in the step 1, sowing tobacco seed in back immerses in the water 1/3 place of seedling-cultivating tray bottom, treats thinning behind the seed sprouting; Make and stay 1 tobacco seedling in every hole, and change water into low-phosphorous nutrient solution, supply the moisture that reduces in the low-phosphorous nutrient solution every day; Per 2 weeks are changed once low-phosphorous nutrient solution, grow to 50 days after, obtain infection rate greater than 30% Mycorrhizal cigarette seedling; Replace the AMF microbial inoculum with the sterilization river sand then, the sowing tobacco seed is according to the cultivation method of Mycorrhizal cigarette seedling; Cultivation obtains non-Mycorrhizal cigarette seedling, and non-Mycorrhizal cigarette seedling is as control treatment, subsequent use;
The transplanting and the management of step 3, Mycorrhizal cigarette seedling
Get field soil, crushing screening is used the formaldehyde fumigation sterilization treatment, and is subsequent use after air-dry 5 days; Sneak into an amount of plumbi nitras in the soil after air-dry, form the soil that the lead pollution level is respectively four kinds of different pollution levels of 0,350,500,1000 mg/kg, represent pollution-free, slight pollution, intermediate pollution, serious pollution respectively; In the soil of different pollution levels, apply corresponding nitrate of lime respectively, make the nitrate nitrogen content under each pollution level identical; The soil of every kind of pollution level is divided into 4 components to be contained in the different basins; Each lead pollution level is made as 4 processing; Be respectively 1 non-Mycorrhizal cigarette seedling and do control treatment (not inoculum, do not add cow dung); 1 Mycorrhizal cigarette seedling is handled, and 1 non-Mycorrhizal cigarette seedling applies cow dung processing, and 1 Mycorrhizal cigarette seedling applies cow dung processing.Concrete operations during the dress basin are: in 1.8 liters plastic basin; Pollution or untainted soil 1.5 kg that every basin dress configures; Every basin is only adorned the soil of a kind of pollution level; Wherein to apply the addition of cow dung in the processing of cow dung be 1% of soil quality for two needs, i.e. 15 g are with cow dung and whole basin soil mixing.4 lead pollution levels, each pollution level are provided with 4 processing, amount to 16 processing, and each processing is made 4 basins and repeated, and every potted plant cigarette seedling 1 strain amounts to 64 basins; Plastic basin randomized arrangement in greenhouse by solar heat is cultivated, and adds chemical fertilizer in the nurturing period, and the addition of chemical fertilizer applies by the convention amount of tobacco planting, and the cigarette seedling carries out pest management simultaneously, waters running water, keeps water capacity about 70%; Transplant results mensuration biological tobacco amount and lead content after 70 days;
The result shows: from table 1, obtain, under all lead pollution levels, in the processed group of inoculation AMF and cow dung, all can promote tobacco growing; Average plant dry weight in three experimental group is 2.5,2.3 and 2.5 times of control group, and the average weight of root system in three experimental group is 2.6,2.7 and 2.7 times of control group, under uncontaminated level; Promote in the control group that the tobacco growing effect is best; And under pollution level, promote the tobacco growing most pronounced effects among the experimental group C
Obtain from table 2, in lead-contaminated soil, apply and reduced in the tobacco plant plumbous residually in the processed group of microbial inoculum and cow dung mixture, but in not having contaminated soil, apply and all can reduce plumbous residual in the tobacco plant in the processed group of microbial inoculum and cow dung mixture.
Table 1 tobacco plant part dry weight (gram)
The lead content (mg/kg) of
table 2 tobacco plant part
embodiment two
Utilize AMF and fertilizer to reduce the residual method of tobacco cadmium, may further comprise the steps:
The preparation of step 1, AMF microbial inoculum
Compound method with step 1 among the embodiment 1;
The cultivation of step 2, Mycorrhizal cigarette seedling
Cultural method with the step 2 among the embodiment 1;
The transplanting and the management of step 3, Mycorrhizal cigarette seedling
Method for transplanting according to step 3 among the embodiment 1; Plumbi nitras is replaced with cadmium nitrate; Form the soil that the cadmium pollution level is respectively four kinds of different pollution levels of 0,1,10,100 mg/kg; Represent pollution-free, slight pollution, intermediate pollution, serious pollution respectively; Under different cadmium pollution levels, apply corresponding calcium nitrate respectively; The back obtains transplanting biomass and the cadmium content in the tobacco after 70 days according to the management method of the step 3 among the embodiment 1;
The result shows: from table 3, obtain; Under all cadmium pollution levels; In the processed group of inoculation AMF and cow dung, all can promote tobacco growing, the average plant dry weight in three experimental group is 2.8-6.0 times of control group, and the average plant dry weight of experimental group C is 5.2 times of control group; Under pollution level, experimental group C promotes the tobacco growing most pronounced effects;
From table 4, obtain; Under the same cadmium pollution level; Inoculation microbial inoculum processed group and apply microbial inoculum with cow dung mixture process group in all can reduce tobacco plant cadmium content partly; Plant part cadmium content in the experimental group in the processed group of inoculation microbial inoculum is 34-89% of a control group, and the plant part cadmium content that applies in the experimental group in microbial inoculum and the cow dung mixture process group is 40-78% of a control group.
Table 3 tobacco plant part dry weight (gram)
The lead content (mg/kg) of
table 4 tobacco plant part
Utilize AMF and fertilizer to reduce tobacco lead, the residual method of cadmium, may further comprise the steps:
The preparation of step 1, AMF microbial inoculum
With the step 1 among the embodiment 1;
The cultivation of step 2, Mycorrhizal cigarette seedling
With the step 2 among the embodiment 1;
The transplanting and the management of step 3, Mycorrhizal cigarette seedling
Method for transplanting according to step 3 among the embodiment 1; In air-dry soil, add plumbi nitras and cadmium nitrate; Form that lead/cadmium pollution level is respectively 0/0,350/1,500/10, the soil of four kinds of different pollution levels of 1000/100mg/kg; Represent pollution-free, slight pollution, intermediate pollution, serious pollution respectively; Under different lead/cadmium pollution level, apply corresponding calcium nitrate respectively; The back obtains transplanting biomass, lead content and the cadmium content in the tobacco after 70 days according to the management method of the step 3 among the embodiment 1;
The result shows: from table 5, obtain; Under all plumbous cadmium combined pollution level conditions; The inoculation microbial inoculum, using cow dung and use microbial inoculum and the cow dung mixture all promotes tobacco growing, is respectively 3.1,1.9 and 4.0 times of control group in the average plant dry weight of each experimental group;
Obtain from table 6; Under same pollution level; The inoculation microbial inoculum, apply cow dung and use microbial inoculum and the cow dung mixture all makes the lead content of tobacco plant part reduce, in experimental group C, apply the lead content that applies cow dung in cow dung, inoculation microbial inoculum and the inoculation microbial inoculum and reduce more than 50%.
Obtain from table 7, under same pollution level, apply cow dung, inoculate microbial inoculum and use microbial inoculum and the cow dung mixture all makes the cadmium content of tobacco plant part reduce, the reduction cadmium residual effect of control group is higher than other three groups;
Table 5 tobacco plant part dry weight (gram)
The lead content (mg/kg) of
table 7 tobacco plant part
The constituent of used low-phosphorous nutrient solution in technique scheme: the KH that contains 11.3 mg in every liter of low-phosphorous nutrient solution
2PO
4, 126.3 mg KNO
3, 295mg Ca (NO
3)
2, 123.3 mg MgSO
4, 0.715 mg H
3BO
3, 0.453 mg MnCl
24H
2The H of O, 0.005 mg
2MoO
4H
2The ZnSO of O, 0.055 mg
47H
2The CuSO of O and 0.02 mg
45H
2O wherein also adds in low-phosphorous nutrient solution by 1.863 mg EDTA and 1.393 mg FeSO
47H
2The chelating attitude Fe-EDTA that O constitutes;
Used tobacco seed in technique scheme is general commercially available prod, and used among the present invention is tobacco K326, buys cigarette seed Co., Ltd in Qingdao;
Used AMF bacterial classification in technique scheme,, nectarine mould for Scotland sacculus do not have that the stalk capsule is mould, sacculus any one in mould in Glomus mosseae or the root, all can on market, buy.
No matter can be found out by above embodiment, be in lead pollution, cadmium pollution or plumbous cadmium combined pollution soil, inoculation AMF microbial inoculum with apply that cow dung all can promote tobacco growing, to reduce plumbous cadmium residual.Even in untainted soil; Inoculate the AMF microbial inoculum and apply cow dung and also can significantly promote tobacco growing; No matter show whether there is heavy metal pollution in the soil, AMF microbial inoculum and cow dung among the present invention all can be applied in the planting process of tobacco.
It should be noted last that: above embodiment is only in order to explanation; And unrestricted technical scheme of the present invention; Although the present invention is specified with reference to the foregoing description; Those of ordinary skill in the art is to be understood that: still can make amendment or be equal to replacement the present invention, and not break away from any modification or the local replacement of the spirit and scope of the present invention, it all should be encompassed in the middle of the claim scope of the present invention.
Claims (2)
1. utilize AMF and fertilizer to reduce tobacco lead, the residual method of cadmium, it is characterized in that: may further comprise the steps:
The preparation of step 1, AMF microbial inoculum
1) to get 3 parts of particle diameters by volume be 1mm river sand and 1 part of peat that particle diameter is 1mm mix, and form mixture, after with mixture under 121 ℃ of conditions, sterilize 50-60 minutes, taking-up is cooled to 30-40 ℃, makes mixed-matrix, and is subsequent use;
2) in the ratio that adds 7.8g urea and 33g superphosphate in every 100kg mixed-matrix, add urea and superphosphate in the mixed-matrix in step 1) after the sterilization, mix, form base fertilizer, subsequent use;
3) get 95-97% base fertilizer and 3-5% initial AMF microbial inoculum by weight percentage, mix, form culture mix; With culture mix weight is benchmark, in culture mix, adds 15-25% water, back sowing clover grass seed; By the conventional cultural method of clover grass, cultivated 3-4 months, obtain clover grass that is infected by AMF and the matrix that contains the outer mycelia of AMF spore and root; The root of clover grass is cut into the segment of 0.5-1mm; Mix with the matrix that contains the outer mycelia of AMF spore and root then, make the AMF microbial inoculum, subsequent use;
The cultivation of step 2, Mycorrhizal cigarette seedling
In the seedling-cultivating tray of floatation of foam hole, add the AMF microbial inoculum that makes in the step 1, sowing tobacco seed in back immerses in the water 1/3 place of seedling-cultivating tray bottom; Treat thinning behind the seed sprouting, make and stay 1 tobacco seedling in every hole, and change water into low-phosphorous nutrient solution; Supply the moisture that reduces in the low-phosphorous nutrient solution every day, per 2-3 weeks are changed once low-phosphorous nutrient solution, grow to 50-60 days after; Get infection rate greater than 30% Mycorrhizal cigarette seedling, subsequent use;
The transplanting and the management of step 3, Mycorrhizal cigarette seedling
In field soil, dig and transplant the cave, the Mycorrhizal cigarette seed that obtains in the step 2 is implanted in transplants in the cave, adopt the method for spreading fertilizer over the fields to apply fertilizer then in the field; Applied amount adds by 15000-22500 kg/ha; Mycorrhizal cigarette seedling is cultivated, added chemical fertilizer in the nurturing period, the addition of chemical fertilizer applies by the convention amount of tobacco planting; Carry out field management such as moisture, weeds simultaneously, make the tobacco normal growth to results;
Described fertilizer is the cow dung of becoming thoroughly decomposed;
The bacterial classification of described AMF microbial inoculum is that Scotland sacculus is mould
Glomuscaledonium90036, nectarine do not have the stalk capsule mould
AcaulosporamelleaZZ, Glomus mosseae
GlomusmosseaeBEG167Or sacculus is mould in the root
GlomusintraradicesBEG141In any one.
2. AMF and the fertilizer of utilizing according to claim 1 reduces that tobacco is plumbous, the residual method of cadmium, it is characterized in that: the constituent of described low-phosphorous nutrient solution: the KH that contains 11.3mg in every liter of low-phosphorous nutrient solution
2PO
4, 126.3mg KNO
3, 295mg Ca (NO
3)
2, 123.3mg MgSO
4, 0.715mg H
3BO
3, 0.453mg MnCl
24H
2The H of O, 0.005mg
2MoO
4H
2The ZnSO of O, 0.055mg
47H
2The CuSO of O and 0.02mg
45H
2O wherein also adds by 1.863mgEDTA and 1.393mgFeSO in low-phosphorous nutrient solution
47H
2The chelating attitude Fe-EDTA that O constitutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210071205 CN102577826B (en) | 2012-03-19 | 2012-03-19 | Method for using arbuscular mycorrhizal fungi and organic fertilizer to reduce residue of lead and cadmium in tobacco |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210071205 CN102577826B (en) | 2012-03-19 | 2012-03-19 | Method for using arbuscular mycorrhizal fungi and organic fertilizer to reduce residue of lead and cadmium in tobacco |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102577826A true CN102577826A (en) | 2012-07-18 |
| CN102577826B CN102577826B (en) | 2013-11-06 |
Family
ID=46467517
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201210071205 Expired - Fee Related CN102577826B (en) | 2012-03-19 | 2012-03-19 | Method for using arbuscular mycorrhizal fungi and organic fertilizer to reduce residue of lead and cadmium in tobacco |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102577826B (en) |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102994394A (en) * | 2012-08-28 | 2013-03-27 | 中节能六合天融环保科技有限公司 | Fungal strain LP-18-3 and application of fungal strain LP-18-3 in lead-containing water body treatment |
| CN103891502A (en) * | 2014-03-13 | 2014-07-02 | 贵州中烟工业有限责任公司 | Method for reducing cadmium content in tobacco leaves |
| CN103990647A (en) * | 2014-05-12 | 2014-08-20 | 华南师范大学 | Method of enhancing cadmium absorption of black nightshade from soil by utilization of arbuscular mycorrhizal fungi |
| CN105557112A (en) * | 2015-12-25 | 2016-05-11 | 中国矿业大学(北京) | Method for improving soil and increasing fertilizer through synergy of covering grass and inoculation |
| CN105580688A (en) * | 2016-01-08 | 2016-05-18 | 华南师范大学 | Method for reducing cadmium accumulation amount and increasing yield of honeysuckle through glomus mosseae |
| CN105660098A (en) * | 2016-01-08 | 2016-06-15 | 华南师范大学 | Method for reducing honeysuckle cadmium accumulation and increasing yield of honeysuckle by means of glomus intraradices |
| CN108160685A (en) * | 2018-01-02 | 2018-06-15 | 山西大学 | A kind of method that plant-microorganism joint repairs coal mining area soil |
| CN109906893A (en) * | 2019-03-18 | 2019-06-21 | 浙江师范大学 | A kind of method that Mycorrhizal higher yield of crops in farmland is high-quality |
| CN109956713A (en) * | 2019-05-13 | 2019-07-02 | 辽宁大学 | A kind of plant cover concrete and its preparation method and application |
| CN110467924A (en) * | 2019-09-09 | 2019-11-19 | 山东胜伟盐碱地科技有限公司 | A kind of alkaline land soil mercury, cadmium ion remover and preparation method thereof |
| CN111758528A (en) * | 2020-06-19 | 2020-10-13 | 中国农业科学院农业资源与农业区划研究所 | Seedling raising substrate and preparation method and application thereof |
| CN113973672A (en) * | 2021-11-23 | 2022-01-28 | 济南大学 | Ecological planting method of American ginseng |
| CN114316997A (en) * | 2021-12-18 | 2022-04-12 | 四川大学 | Reagent for circularly repairing cadmium pollution in rice field and preparation method thereof |
| CN114747437A (en) * | 2022-04-12 | 2022-07-15 | 浙江省农业科学院 | Method for relieving cadmium toxicity of tomatoes by using arbuscular mycorrhizal fungi and application |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1482106A (en) * | 2002-09-10 | 2004-03-17 | 上海百成生物科技有限公司 | Compound biological bacterium fertilizer |
| CN101947545A (en) * | 2010-09-27 | 2011-01-19 | 河南科技大学 | Method for biologically restoring organic phosphorus pesticide polluted soil |
| CN102047808A (en) * | 2010-10-29 | 2011-05-11 | 河南科技大学 | Method for reducing heavy metal residues in tobacco by use of arbuscular mycorrhizal fungi |
| CN102057826A (en) * | 2010-10-18 | 2011-05-18 | 河南科技大学 | Method for reducing vegetable phoxim residue by utilizing glomus mosseae or glomus intraradices |
-
2012
- 2012-03-19 CN CN 201210071205 patent/CN102577826B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1482106A (en) * | 2002-09-10 | 2004-03-17 | 上海百成生物科技有限公司 | Compound biological bacterium fertilizer |
| CN101947545A (en) * | 2010-09-27 | 2011-01-19 | 河南科技大学 | Method for biologically restoring organic phosphorus pesticide polluted soil |
| CN102057826A (en) * | 2010-10-18 | 2011-05-18 | 河南科技大学 | Method for reducing vegetable phoxim residue by utilizing glomus mosseae or glomus intraradices |
| CN102047808A (en) * | 2010-10-29 | 2011-05-11 | 河南科技大学 | Method for reducing heavy metal residues in tobacco by use of arbuscular mycorrhizal fungi |
Non-Patent Citations (3)
| Title |
|---|
| 王发园等: "丛枝菌根-植物修复重金属污染土壤研究中的热点", 《生态环境》 * |
| 王茂胜等: "烟草丛枝菌根(AM)研究进展", 《中国土壤与肥料》 * |
| 胡文友等: "无公害蔬菜生产中重金属含量的控制技术", 《农业环境科学学报》 * |
Cited By (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102994394B (en) * | 2012-08-28 | 2014-12-31 | 中节能六合天融环保科技有限公司 | Fungal strain LP-18-3 and application of fungal strain LP-18-3 in lead-containing water body treatment |
| CN102994394A (en) * | 2012-08-28 | 2013-03-27 | 中节能六合天融环保科技有限公司 | Fungal strain LP-18-3 and application of fungal strain LP-18-3 in lead-containing water body treatment |
| CN103891502A (en) * | 2014-03-13 | 2014-07-02 | 贵州中烟工业有限责任公司 | Method for reducing cadmium content in tobacco leaves |
| CN103990647A (en) * | 2014-05-12 | 2014-08-20 | 华南师范大学 | Method of enhancing cadmium absorption of black nightshade from soil by utilization of arbuscular mycorrhizal fungi |
| CN105557112A (en) * | 2015-12-25 | 2016-05-11 | 中国矿业大学(北京) | Method for improving soil and increasing fertilizer through synergy of covering grass and inoculation |
| CN105580688A (en) * | 2016-01-08 | 2016-05-18 | 华南师范大学 | Method for reducing cadmium accumulation amount and increasing yield of honeysuckle through glomus mosseae |
| CN105660098A (en) * | 2016-01-08 | 2016-06-15 | 华南师范大学 | Method for reducing honeysuckle cadmium accumulation and increasing yield of honeysuckle by means of glomus intraradices |
| CN105580688B (en) * | 2016-01-08 | 2019-05-28 | 华南师范大学 | A method of honeysuckle Cd accumulation is reduced using Glomus mosseae and improves its yield |
| CN105660098B (en) * | 2016-01-08 | 2019-05-28 | 华南师范大学 | A method of honeysuckle Cd accumulation is reduced using Glomus intraradices and improves its yield |
| CN108160685B (en) * | 2018-01-02 | 2020-02-14 | 山西大学 | Method for repairing coal mining area soil by combining plants and microorganisms |
| CN108160685A (en) * | 2018-01-02 | 2018-06-15 | 山西大学 | A kind of method that plant-microorganism joint repairs coal mining area soil |
| CN109906893A (en) * | 2019-03-18 | 2019-06-21 | 浙江师范大学 | A kind of method that Mycorrhizal higher yield of crops in farmland is high-quality |
| CN109906893B (en) * | 2019-03-18 | 2022-02-01 | 浙江师范大学 | Method for high yield and high quality of farmland mycorrhizal crops |
| CN109956713A (en) * | 2019-05-13 | 2019-07-02 | 辽宁大学 | A kind of plant cover concrete and its preparation method and application |
| CN110467924A (en) * | 2019-09-09 | 2019-11-19 | 山东胜伟盐碱地科技有限公司 | A kind of alkaline land soil mercury, cadmium ion remover and preparation method thereof |
| CN111758528A (en) * | 2020-06-19 | 2020-10-13 | 中国农业科学院农业资源与农业区划研究所 | Seedling raising substrate and preparation method and application thereof |
| CN113973672A (en) * | 2021-11-23 | 2022-01-28 | 济南大学 | Ecological planting method of American ginseng |
| CN114316997A (en) * | 2021-12-18 | 2022-04-12 | 四川大学 | Reagent for circularly repairing cadmium pollution in rice field and preparation method thereof |
| CN114747437A (en) * | 2022-04-12 | 2022-07-15 | 浙江省农业科学院 | Method for relieving cadmium toxicity of tomatoes by using arbuscular mycorrhizal fungi and application |
| CN114747437B (en) * | 2022-04-12 | 2023-08-25 | 浙江省农业科学院 | Method for relieving toxic action of cadmium in tomatoes by using arbuscular mycorrhizal fungi and application |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102577826B (en) | 2013-11-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102577826B (en) | Method for using arbuscular mycorrhizal fungi and organic fertilizer to reduce residue of lead and cadmium in tobacco | |
| CN102047808B (en) | Method for reducing heavy metal residues in tobacco by use of arbuscular mycorrhizal fungi | |
| CN105859479A (en) | Tobacco field soil improving method | |
| CN103125251A (en) | Application method of arbuscular mycorrhizal fungus in large-scale tobacco cultivation | |
| CN105028123A (en) | Organic matter-enriched brackish water irrigation method for saline-alkali soil and application of irrigation method in planting of silphium perfoliatum | |
| CN101683064B (en) | Soilless culture substrate and application thereof | |
| CN105940893B (en) | The fast breeding method of fruit of Rangoon creeper's seedling | |
| CN101194585A (en) | Raise seedling method of jatropha mycorhiza | |
| CN107353116A (en) | A kind of selenium-rich water east leaf mustard implantation methods | |
| CN108738442B (en) | Tea-oil camellia forest soil curing system and manufacturing method thereof | |
| CN112974510A (en) | Application of ciliate desert-grass in repairing heavy metal arsenic pollution of soil in mine | |
| CN1322816C (en) | Method for establishing exogenous microorganism onto plant root system | |
| CN107211820A (en) | A kind of Cuiguan pear cuttage planting method for improving yield | |
| CN111771446A (en) | Method for promoting improvement of agilawood planting soil | |
| CN102077763A (en) | Biologic culture medium of seedlings | |
| CN101569284A (en) | Preparation method of mycorrhizal tobacco seedling by taking sand as ground substance | |
| CN101218929A (en) | Application of earthworm faeces, compost or earthworm faeces-compost leaching liquor soaked seeds in lawn plant | |
| CN110583460A (en) | Soilless culture method for okra | |
| CN112521231A (en) | Soil improvement bacterial fertilizer | |
| CN110036839A (en) | A kind of control method of tomato verticillium wilt | |
| KR100430761B1 (en) | Method for producing high-density antagonistic microbes base material | |
| CN109328959A (en) | A kind of cultural method of cuckoo new varieties | |
| CN107926508A (en) | The fast breeding method of tuber of pinellia seedling | |
| CN107466774A (en) | A kind of method for fast growing of podocarpus | |
| CN109287416B (en) | Drought-resisting training planting method for rice |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131106 Termination date: 20140319 |