CN101569284A - Preparation method of mycorrhizal tobacco seedling by taking sand as ground substance - Google Patents

Abstract

The invention discloses a preparation method of mycorrhizal tobacco seedling by taking sand as ground substance, which belongs to the field of fire-cured tobacco seedling culture and aims at providing the preparation method of mycorrhizal tobacco seedlings with low cost and without pollution to the environment. The technical proposal is as follows: sand with certain grain size is selected to be sterilized at high temperature, after the sand is cooled, microbial inoculum is added and evenly mixed with the sand, and the mixture is put into a seedling tray; after being planted, a floating seedling culture tray is placed in Hoagland nutrient fluid for culture of seedling according to the conventional method, and the distance between the fluid level of the nutrient fluid and the top of the seedling tray is 2-4cm; Hoagland nutrient fluid with different intensities is adopted according to different periods in the culture of seedling. The invention has the advantages of wide source of raw materials, environmental protection, small dosage of strains, high quality of tobacco seedling, high infection ratio of tobacco seedling mycorrhizal fungi, large proportion of infection, low cost, strong adaptability, easy popularization and the like; the invention is a fire-cured tobacco seedling culture method which is very beneficial to the environment.

Description

With sand is the production method of matrix Mycorrhizal cigarette seedling

Technical field:

The present invention relates to a kind of flue-cured tobacco seedling-cultivating field, relate in particular to a kind of production method of Mycorrhizal cigarette seedling.

Background technology:

As everyone knows, arbuscular mycorrhizal fungi (Arbuscular mycorrhizalfungus) distributes extremely extensive at occurring in nature, it can form mycorhiza with the plant of land more than 80%, can be by different way or approach influence many metabolic processes of plant, not only growth and development of plant, nutrition condition, resistance and yield and quality etc. are had important function, and the structure etc. of can improving the soil to a certain extent.

Can tobacco be one of arbuscular mycorrhizal fungi aulophyte, in cured tobacco production, can reduce the cigarette seedling of chemical fertilizer and applications of pesticide amount after providing healthy and strong under the situation about reducing cost, transplanting, and will directly influence the economic benefit of cured tobacco production process.Arbuscular mycorrhizal fungi and tobacco root form after the mycorhiza, can not only improve tobacco to the absorption of mineral elements such as N.P.K, improve tobacco resistance, enhance disease resistance, improve the tobacco rhizosphere microenvironment, and can improve its output and improve quality (Liu Jiang etc. the arbuscular mycorrhiza mycorrhizal fungi is flat down to the influence [J] of tobacco production and value amount at different phosphate rich water. Yunnan University's journal (natural science edition), 1999,21 (3): 239-242. Liu Yan honor etc. the screening of good arbuscular mycorrhiza mycorrhizal fungi [J] on the tobacco. Shandong Agricultural University's newspaper, 1997,28 (3): 269-273.).Studies show that according to relevant: transplant mycorhiza cigarette seedling, can become the outer mycelia of a large amount of roots, nutrition status of the plant and biomass all are significantly higher than the cigarette seedling of inoculation when transplanting and do not inoculate cigarette seedling (Jiang Long etc., the growth and the effect study thereof of mycorhiza after the transplantation of seedlings of mycorhiza cigarette, the biological journal of mountain farming, 2009,28 (1): 1-4,2009).

At present, the matrix that tobacco seedling adopted on producing is mainly mixed according to a certain percentage by the peat composed of rotten mosses, vermiculite and perlite; And the peat composed of rotten mosses is as a kind of non-renewable resource, cost height not only, and also a large amount of exploitation also can cause the heavy damage of ecotope.For this reason, researcher has carried out a large amount of explorations; For example, publication number discloses a kind of prescription of Mycorrhizal cigarette seedling matrix and the nutrient solution prescription of Mycorrhizal cigarette seedling respectively for the patent application of " CN101297632A " and " CN101213928A ", but above-mentioned two used matrix of patent application are not still left peat; In addition, publication number for " CN101422122A " and " CN1799308A " though patent application disclose and utilize sand grains to carry out flue-cured tobacco seedling-cultivating for matrix, it is not the mycorhiza seedling that the cigarette seedling is educated by institute, certain feature that does not possess the mycorhiza seedling.At present, sand low with cost, wide material sources are matrix, and the technology of utilizing arbuscular mycorrhizal fungi to carry out the production of cigarette seedling there is no report both at home and abroad.

Summary of the invention:

At the above-mentioned defective that exists in the prior art, the present invention aims to provide that a kind of cost is low, what do not destroy environment is the production method of matrix Mycorrhizal cigarette seedling with sand.

To achieve these goals, the present invention by the following technical solutions: it comprises matrix treatments, microbial inoculum inoculation, nutrient management; Concrete grammar is as follows:

1) gets 180 ℃ in the sand sterilization two hours that particle diameter is 2～4mm, cooling;

2) cooled sand 5.5Kg is evenly mixed the floating seedlings dish in 520 * 330 * 60mm that packs into, 160 holes with microbial inoculum 20g;

3) sow 2～3 pelleted seeds in each hole, carry out capping with the sand of particle diameter≤2mm;

4) place the Hoagland nutrient solution to grow seedlings according to a conventional method on the floating seedlings dish, control nutrient solution liquid level is apart from seedling dish top 2～4cm; Wherein, from sowing the Hoagland nutrient solution that adopts 30% intensity to thinning, adopt the Hoagland nutrient solution of 50% intensity from thinning to leaf-cutting for the first time, from the leaf-cutting first time to transplanting the Hoagland nutrient solution that adopts 100% intensity.

Described microbial inoculum expands numerous forming by Glomus mosseae (Glomus mosseae).

In technique scheme, Glomus mosseae (Glomus mosseae) is respectively from Inst. of Plant Nutrition ﹠. Resource, Beijing City Academy of Agricultural ﹠. Fo's (numbering: BGCGZ01A is called for short GM01) and Guizhou Province Tabacco Scientific Research Institute (GM02).The grow seedlings nutrient solution prescription such as the table 1 of different times:

The nutrient solution concentration of the table 1:Hoagland nutrient solution and the different times of growing seedlings

Annotate: Hoagland nutrient solution in the table (Liu Runjin etc., mycorhiza is learned, Science Press, 2007.)

Compared with the prior art, the present invention has been owing to replaced peat with sand, and adopted technique scheme, therefore has following advantage:

(1) extensive, the environmental friendliness of raw material sources;

(2) few, the cigarette seedling quality height of bacterial classification dosage; Cigarette seedling mycorrhizal fungi infection rate 〉=76.67% infects ratio 〉=90%;

(3) with low cost, adaptability strong, be convenient to promote.

Facts have proved that adopt the inventive method to educate the cigarette seedling, the mean value of its each technical indicator all is better than nonvaccinated cigarette seedling.Below be to get 10 strain cigarette seedlings at random to carry out the biological character survey data:

Table 2: mycorrhizal fungi is to the influence of cigarette seedling economical character

As can be seen from Table 2, adopt the inventive method to educate the cigarette seedling, its average root cap score you can well imagine high 15.37% and 14.42%, and on average stem girth has improved 14.61% and 11.69% respectively, and all the other every technology item indexs also have raising by a relatively large margin.

Accompanying drawing is said name

Fig. 1 is that the cigarette seedling becomes the spore figure of cigarette shoot root portion in seedling stage;

Fig. 2 is that the cigarette seedling becomes cigarette shoot root portion mycelia in seedling stage.

Embodiment:

The invention will be further described below in conjunction with specific embodiment:

1) getting particle diameter is that the sand of 2～4mm removes mud and foreign material, 180 ℃ of sterilizations two hours, cooling;

2) cooled sand 5.5Kg is evenly mixed the floating seedlings dish in 520 * 330 * 60mm that packs into, 160 holes with microbial inoculum 20g; Pier tight sand body, make it closely knit so that moisture absorption is wipeed card off with batten then;

3) sow 2～3 pelleted seeds in each hole, the surface with the sand of particle diameter≤2mm evenly spreads the Gai Zaimiao dish guarantees smooth, the health in seedbed;

4) place the Hoagland nutrient solution to grow seedlings according to a conventional method on the floating seedlings dish, control nutrient solution liquid level is apart from seedling dish top 2～4cm; Wherein, approximately need 30 days, adopt the Hoagland nutrient solution of 30% intensity during this period from being seeded into thinning; Approximately need 25 days from thinning to leaf-cutting for the first time, adopt the Hoagland nutrient solution of 50% intensity during this period; Need 25 days from the leaf-cutting first time to transplanting approximately, adopt the Hoagland nutrient solution of 100% intensity during this period.From being seeded into transplanting about altogether 80 days, seedling stage, temperature influence was approximately 55～65 days.From Fig. 1, Fig. 2 as can be seen: become seedling stage, can observe arbuscular mycorrhizal fungi spore and a large amount of mycelia formation at the root of cigarette seedling.

Claims (2)

1. one kind is the production method of matrix Mycorrhizal cigarette seedling with sand, comprises matrix treatments, microbial inoculum inoculation, nutrient management; It is characterized in that concrete grammar is as follows:

1) gets 180 ℃ in the sand sterilization two hours that particle diameter is 2～4mm, cooling;

2) cooled sand 5.5Kg is evenly mixed the floating seedlings dish in 520 * 330 * 60mm that packs into, 160 holes with microbial inoculum 20g;

3) sow 2～3 pelleted seeds in each hole, carry out capping with the sand of particle diameter≤2mm;

4) place the Hoagland nutrient solution to grow seedlings according to a conventional method on the floating seedlings dish, control nutrient solution liquid level is apart from seedling dish top 2～4cm; Wherein, from sowing the Hoagland nutrient solution that adopts 30% intensity to thinning, adopt the Hoagland nutrient solution of 50% intensity from thinning to leaf-cutting for the first time, from the leaf-cutting first time to transplanting the Hoagland nutrient solution that adopts 100% intensity.

2. according to claim 1 is the production method of matrix Mycorrhizal cigarette seedling with sand, it is characterized in that: described microbial inoculum expands numerous forming by Glomus mosseae (Glomus mosseae).

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