CN108358695A - A kind of cabbage formula fertilizer special and preparation method thereof - Google Patents
A kind of cabbage formula fertilizer special and preparation method thereof Download PDFInfo
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- CN108358695A CN108358695A CN201810195796.9A CN201810195796A CN108358695A CN 108358695 A CN108358695 A CN 108358695A CN 201810195796 A CN201810195796 A CN 201810195796A CN 108358695 A CN108358695 A CN 108358695A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/30—Layered or coated, e.g. dust-preventing coatings
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/10—Solid or semi-solid fertilisers, e.g. powders
- C05G5/12—Granules or flakes
Abstract
The invention belongs to agricultural fertilizer production technical fields, are related to a kind of cabbage formula fertilizer special, including the following raw material in parts by weight：60 80 parts of amino acid, 60 80 parts of humic acid, 5 10 parts of insect nuclear polyhedrosis virus, 40 60 parts of dregs of beans, 208 255 parts of urea, 108 174 parts of industrial grade monoammonium phosphate, 80 110 parts of potassium sulfate, 0.5 1 parts of complex microorganism bacterium solution；In fertilizer, organic matter 20% 40%, the sum of N, P, K three 19% 40%, composite microbial bacteria >=0.2 hundred million/g.The present invention also provides a kind of preparation methods of above-mentioned cabbage formula fertilizer special, and the measurement of physicochemical property is carried out to planting ground soil, and cabbage breeding time regulation of fertilizer requirement calculates the magnitude of recruitment of N, P, K needed for cabbage；It is mixed to prepare fertiliser granulates after raw material in addition to complex microorganism bacterium solution and dregs of beans is crushed respectively；Complex micro organism fungicide obtained and coating oil are sprayed on fertiliser granulates surface after mixing.The cabbage formula fertilizer special of the present invention can reduce cost, increase yield, improve quality.
Description
Technical field
The invention belongs to agricultural fertilizer production technical fields, and in particular to a kind of cabbage formula fertilizer special and its preparation
Method.
Background technology
Cabbage is generally planted on fertile sandy loam to the soil of clay loam, it is fertilizer-favorable plant, is sought to soil
The uptake for supporting element is more than general vegetables, often produces 1000 kilograms of wild cabbages, needs to absorb purity nitrogen 4.1-6.5 public affairs from soil
Jin, 0.5-0.8 kilograms of phosphorus pentoxide, 4.1-5.7 kilograms of potassium oxide, 3.5-4.5 kilograms of calcium, sulphur and boron send out the growth of wild cabbage
It educates and also plays an important role.The inorganic fertilizer that cultivation wild cabbage uses at present is mostly chemical fertilizer of simple substance and matches unreasonable composite fertilizer,
Need fertilising multiple during growth, manipulation strength is big, and the composite fertilizer that chemical fertilizer of simple substance is unreasonable with proportioning, nutrient are single
Or proportioning is uncoordinated, the demand of wild cabbage growth is much not achieved in fertilizer efficiency.
China is agricultural production big country, very high with the utilization of fertilizer, and the requirement to fertilizer is also constantly promoted, currently,
Inorganic fertilizer is largely used, the harmful substance for seriously resulting in crops in this way increases, poor quality, hypotrophy, economic benefit
Difference, Rate of Organic Fertilizer is less in current vegetable growing, and chemical dosage is more, and improper fertilization will produce fertilizer damage, not only reduce yield
With quality, can also pest and disease damage be made to increase, destroy soil texture, or even pollution environment.The fertilising of vegetables there are the problem of it is more tight
Weight, is mainly manifested in the following aspects：Organic fertilizer is insufficient, Application Ratio is improper, dose is improper, fertilizing time is unreasonable,
Fertilizing method it is improper it is equal blindly apply fertilizer, caused by yield decline, pest and disease damage is serious.In consideration of it, while ensureing yield, more answer
Using rational formulated fertilization method, the production cost of fertilising is reduced, is increased economic efficiency, maintained its sustainable and stable development, carry
High utilization rate of fertilizer, is effectively improved ecological environment of soil, and microbial organic/inorganic fertilizer is arranged as a kind of important agricultural technology
The effect applied in developing an agriculture featuring high yields, fine quality and high efficiency is increasingly recognized by people, this to its exploitation, popularization, using carrying
Sizable development space is supplied.
Invention content
In order to overcome above-mentioned the shortcomings of the prior art, the purpose of the present invention is overcome in production to apply due to unreasonable
Fertilizer applies the deficiencies of reasons such as chemical nitrogen fertilizer cause yield reduction, quality decline partially, provides a kind of special with cabbage nutrient demand
The cabbage formula fertilizer special and preparation method thereof that property matches, can improve yield and improve quality.
To achieve the above object, the present invention provides a kind of cabbage formula fertilizer special, including in parts by weight as follows
Raw material：60-80 parts of amino acid, 60-80 parts of humic acid, 5-10 parts of insect nuclear polyhedrosis virus, 40-60 parts of dregs of beans, urea
208-255 parts, 108-174 parts of industrial grade monoammonium phosphate, 80-110 parts of potassium sulfate, 0.5-1 parts of complex microorganism bacterium solution；Fertilizer
In, the mass content of organic matter is 20%-40%, and the mass content of the sum of N, P, K three is 19%-40%, composite microbial bacteria >=
0.2 hundred million/g.
Further, including in parts by weight the following raw material：60 parts of amino acid, 70 parts of humic acid, insect caryogram are polygonal
5 parts of precursor virus, 40 parts of dregs of beans, 208 parts of urea, 108 parts of industrial grade monoammonium phosphate, 80 parts of potassium sulfate, complex microorganism bacterium solution 0.5
Part；In fertilizer, the mass content of organic matter is 30%-40%, and the mass content of the sum of N, P, K three is 26%-40%, composite microbial
Object bacterium >=0.2 hundred million/g.
Further, the complex microorganism bacterium solution includes bacillus subtilis bacterium solution, bacillus megaterium bacterium solution and glue
Matter bacillus bacterium solution；Bacillus subtilis in the complex microorganism bacterium solution, bacillus megaterium and colloid bacillus cereus
Mass ratio is 1-1.2：1-1.4：1-1.6.
Preferably, bacillus subtilis in the complex microorganism bacterium solution, bacillus megaterium and colloid bacillus cereus
Mass ratio is 1：1.4：1.6.
Further, the weight ratio of N, P, K are 11-20 in special fertilizer：2-10：6-16.
Preferably, the weight ratio of N, P, K are 14 in special fertilizer：4：8.
Further, the mass ratio of the complex microorganism bacterium solution and dregs of beans is 1-2：8-10.
Bacillus subtilis is used in the complex microorganism bacterium solution of the present invention（Bacillussubtilis）, the life of strain
Object characteristic is that thalline is rod-shaped, and to produce gemma aerobic bacteria, gemma is resistant to high fever（It remains to survive within 30 minutes at 100 DEG C）, and
It can be grown in withered grass leachate, gemma is oval or long barrel shape, 0.6~0.9 × 1.0~1.5 μm；In sporangium center or
Nearly central portion is formed；Generally germinate in loins；Rod-shaped 0.3~0.7 × 2~3 μm of vegetative cell isolates or at short chain, rod end half
It is round；Bacterium colony is invasiveness, scabriculous or folding, greyish white, yellowish or even yellow；When growing in liquid medium, wrinkle is commonly formed
Mould；Available protein, a variety of sugar and starch, decompose tryptophan and form indoles；20-55 DEG C of growth temperature, optimum growth temp
37 DEG C, optimal pH 7.1.
Bacillus megaterium is also used in the complex microorganism bacterium solution of the present invention（Bacillusmegaterium), bud is produced
Spore, rod-shaped, end circle.Individually or in short chain arrange.1.2~1.5 × 2.0~4.0 μm.1.0~1.2 × 1.5~2.0 μ of gemma
M, ellipse, middle life or the life of secondary end.
Colloid bacillus cereus is also used in the complex microorganism bacterium solution of the present invention
（BacillusmucilaginosusKrassilnikov）, thick elongated rod shape, end is round, and size is 1 ~ 1.2 × 4 ~ 7 μm.Culture
Later stage forms coarse long ellipse brood cell in long rod thalline, and size is 1.5 ~ 1.8 × 3 ~ 3.5 microns, raw during gemma is more
Or it is not in fusiformis that proximal end, which is given birth to after cyst expands,.
The present invention also provides a kind of preparation methods of above-mentioned cabbage formula fertilizer special, include the following steps：
1）Carry out the measurement of physicochemical property to the soil of cabbage planting site, including but not limited to soil alkali-hydrolyzable nitrogen, rapid available phosphorus,
Available potassium, pH, EC value, boron, calcium；
2）According to the measurement result of above-mentioned physicochemical property and cabbage breeding time regulation of fertilizer requirement, calculated by Stanford Formula
Go out the magnitude of recruitment of N, P, K needed for cabbage；
3）Bacillus subtilis bacterium solution, bacillus megaterium bacterium solution and colloid bacillus cereus bacterium solution are prepared respectively；
4）Bacillus subtilis bacterium solution, bacillus megaterium bacterium solution and colloid bacillus cereus bacterium solution are proportionally carried out mixing
To complex microorganism bacterium solution；
5）Complex microorganism bacterium solution is uniformly mixed with the dregs of beans of sterilized postcooling, low temperature drying obtains composite microbial bacteria
Agent；
6）After raw material in addition to complex microorganism bacterium solution and dregs of beans is crushed respectively, mix in proportion and through low temperature,
Drying, cooling, screening are granulated, and obtain fertiliser granulates；
7）By step 5）In complex micro organism fungicide obtained and coating oil be sprayed on step 6 after mixing）In fertilizer obtained
Expect particle surface, obtains target cabbage formula fertilizer special.
Further, the step 3）Middle bacillus subtilis bacterium solution, bacillus megaterium bacterium solution and colloid bacillus cereus
Bacterium solution is prepared according to following other method：
First bacterial strain is crossed access solid slope culture medium, 32 DEG C of -37 DEG C of biochemical cultivation case stationary cultures 36 hours；Use sterile water
Inclined-plane is rinsed, bacteria suspension is formed；1 volume bacteria suspension is accessed in the fluid nutrient medium of 50 volumes, 32 DEG C -37 DEG C aerobic cultures are extremely
Cell concentration is 1010A/ml orders of magnitude stop culture, obtain bacterium solution；Wherein solid slope culture medium：Peptone 5g/L, chlorination
Sodium 5g/L, beef extract 5g/L, agar 1.5%-2.0%, PH are adjusted to 6.8-7.2；Fluid nutrient medium：Without agar, other compositions and
Content is consistent with solid slope culture medium, and PH is adjusted to 6.5-7.5.
Compared with prior art, the invention has the advantages that：
（1）Cabbage formula fertilizer special provided by the invention is special for the growth characteristics and nutritional need of leaf vegetables cabbage
Property and a biologic organic, inorganic formula fertilizer prepared, including complex microorganism bacterium solution, organic fertilizer and inorganic fertilizer；According to
The Features of Nutrient Absorption and Soil Available Nutrition of wild cabbage are determined best by the statistical analysis of field test results
Formula and formula rate, have both microbial-bacterial fertilizer, organic fertilizer, the triple function of inorganic fertilizer；
（2）Cabbage formula fertilizer special preparation method provided by the invention determines that raw material is matched according to soil nutrient test result
Than new microbial bacterial agent being prepared using new cultural method, then unclassified stores is crushed respectively, is mixed in proportion
It closes, mixing and after low temperature, drying, cooling, screening are granulated is added above-mentioned compound micro- made of fermented absorption using coating oil
The cabbage specific complex microbial organic/inorganic fertilizer of the present invention is made in bacteria agent；
（3）Insect nuclear polyhedrosis virus in the cabbage formula fertilizer special of the present invention can not only be provided to cabbage
Organic nutrient, moreover it is possible to improve the ability of disease and insect resistance；
（4）The cabbage formula fertilizer special of the present invention can reduce cost, increase yield, improve quality, similar to improve
The market competitiveness of product.
Specific implementation mode
The following is a clear and complete description of the technical scheme in the embodiments of the invention, it is clear that described embodiment
Only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, the common skill in this field
All other embodiment that art personnel are obtained without making creative work belongs to the model that the present invention protects
It encloses.
The embodiment of the present invention provides a kind of cabbage formula fertilizer special, including the following raw material in parts by weight：Amino
60-80 parts sour, 60-80 parts of humic acid, 5-10 parts of insect nuclear polyhedrosis virus, 40-60 parts of dregs of beans, 208-255 parts of urea, work
108-174 parts of industry grade monoammonium phosphate, 80-110 parts of potassium sulfate, 0.5-1 parts of complex microorganism bacterium solution；The technical indicator of fertilizer is：
The mass content of organic matter is 20%-40%, and the mass content of the sum of N, P, K three is 19%-40%, composite microbial bacteria >=0.2
Hundred million/g.
By adding humic acid, amino acid and dregs of beans these natural materials, complex microorganism bacterium solution and our unit's research and development
The insect nuclear polyhedrosis virus of production, nutrient that can be in effective activation soil significantly improve the resistance of crop and anti-
The ability of pest and disease damage enhances crop plant cells vigor, accelerates the growth and development of rhizobium and root system, promotes crop root flourishing；
The physiological diseases such as root system is small, root nodule is few, plant is short and small, withered and yellow, tipburn caused by continuous cropping, nutritional deficiency and other diseases to wild cabbage
There is extremely strong preventive effect；Can effective solution be currently used for existing for the fertilizer of cabbage that chemical fertilizer application is excessive, needle
It, nutrient unbalanced, existing microbial manure application effect unstable the problems such as poor to property, can apparent increasing both production and income after.
Further, the complex microorganism bacterium solution includes bacillus subtilis bacterium solution, bacillus megaterium bacterium solution and glue
Matter bacillus bacterium solution；Bacillus subtilis in the complex microorganism bacterium solution, bacillus megaterium and colloid bacillus cereus
Mass ratio is（1-1.2）：（1-1.4）：（1-1.6）.The complex microorganism bacterium solution is by bacillus subtilis
（Bacillussubtitles）, bacillus megaterium（Bacillusmegaterium）, colloid bacillus cereus
（BacillusmucilaginosusKrassilnikov）It is fermented respectively that corresponding microbial inoculum is made, according still further to certain
Weight ratio mixing after form.
Preferably, bacillus subtilis in the complex microorganism bacterium solution, bacillus megaterium and colloid bacillus cereus
Optimum quality ratio is 1：1.4：1.6, the effect of fertilizer under the ratio is best, and the quality of cabbage is best.
The bacillus megaterium contained in the complex microorganism bacterium solution is phosphorus-solubilizing bacteria, and with breeding, quick, vitality is strong, peace
The features such as Atoxic, dissolves and is not easy the compounds such as the calcium phosphorus absorbed, iron phosphorus, aluminium phosphorus in soil crop, promotes invalid phosphorus in soil
Dissolving and utilization；It can also grow and generate a large amount of protease, amino acid, basic element of cell division etc., enhance crop plant cells vigor,
Accelerate crop metabolism, increases Hua Zengguo, Fruit is fast, in good taste, increases substantially the yield and agricultural product grade of crop；
It secretes a large amount of high activity chitinases, quickly removes pathogenic bacteria, accelerates the growth and development of rhizobium and root system, promotes root of the crop
System is flourishing, to crop, root system is small, root nodule is few, plant is short and small caused by continuous cropping and other diseases, withered and yellow, floral leaf, fallen leaves, fallen flowers
Equal physiological diseases have extremely strong preventive effect；Promote crop leaf greening, thicken, robust plant, take root depth, cold-resistant jelly, drought resisting,
Resistant to lodging, premature senescence resistance, anti-continuous cropping；Iturin, polymyxins, the mildew making generated in bacillus subtilis growth course
The polypeptide active substances such as element, bacitracin and surfactin etc. have apparent inhibiting effect to pathogenic bacteria；And colloid bud
Spore bacillus can decomposite potassium, silicon in the mineral such as feldspar, mica, can also decomposite the phosphorus in apatite, have Soluble phosphorus, Potassium release
With fixed nitrogen function, while organic acid, amino acid, polysaccharide, hormone etc. can be generated during growth and breeding and be conducive to plant absorption
With the substance utilized.Endobacillary potassium is after thalline death, separate out, and can be absorbed and used by plants；As microorganism fertilizer
Soil available nitrogen, phosphorus content can be improved in a kind of critical function bacterium in material, improves the multi-effects such as crop yield and quality.It is logical
It crosses laboratory antagonism Inhibition test and field trial is obtained when bacillus subtilis, bacillus megaterium, colloid bacillus cereus
Three kinds of strains have the function of cooperateing with mutually when mixing, more preferable than the effect of single use.
Further, the weight ratio of N, P, K are 11-20 in special fertilizer：2-10：6-16.Preferably, the weight ratio of N, P, K
It is 14：4：8.
Further, the mass ratio of the complex microorganism bacterium solution and dregs of beans is 1-2：8-10.Preferably, complex microorganism
The mass ratio of bacterium solution and dregs of beans is 1:10.
The present invention also provides a kind of preparation methods of above-mentioned cabbage formula fertilizer special, include the following steps：
1）Carry out the measurement of physicochemical property to the soil of cabbage planting site, including but not limited to soil alkali-hydrolyzable nitrogen, rapid available phosphorus,
Available potassium, pH, EC value, boron, calcium etc., then analyze physicochemical property, pointedly supplement the nutrient needed for wild cabbage,
Realize various nutrient balance supplies.Show that soil effective element Plentiful-lack index is as follows by a large amount of data analysis measurement：
The big moderate-element effective content Plentiful-lack index of 1 soil of table（mg/kg）
2）According to the measurement result of above-mentioned physicochemical property and cabbage breeding time regulation of fertilizer requirement, calculated by Stanford Formula
Go out nutrient i.e. N, P, the K supplemented needed for cabbage（Nitrogen, phosphorus, potassium）Magnitude of recruitment be N11-20kg, P2O52-10kg, K2O6-
16kg；
By studying entire wild cabbage breeding time regulation of fertilizer requirement and obtaining applying for different output and different plot using statistics
Fertilizer amount is as follows：
2 cabbage fertilizing recommendation tables of table
We preferentially select yield level to be illustrated in the sprouting broccoli kind of 4500-5500kg herein：In the table of comparisons 1
" the big moderate-element effective content Plentiful-lack index of soil " can be calculated by Stanford Formula and be supplemented into growing area soil
Pure N is 13-15kg, P2O54-6kg, K2O8-10kg。
3）Bacillus subtilis bacterium solution, bacillus megaterium bacterium solution and colloid bacillus cereus bacterium solution are prepared respectively；
Access that bacillus subtilis, bacillus megaterium and colloid bacillus cereus bacterial strain in freezing pipe is crossed respectively is oblique
Face culture medium（A solid mediums）, 37 DEG C of biochemical cultivation case stationary culture cultures 36 hours；It is rinsed with 5ml sterile salines
Inclined-plane forms bacteria suspension；1ml bacteria suspensions are accessed in 250ml triangular flasks, the triangular flask fluid nutrient medium containing 50ml（B liquid is trained
Support base）, using constant-temperature table in 37 DEG C, 150 ~ 200rpm is cultivated 24 hours or more, thalline content is detected, until cell concentration is
1010A/ml orders of magnitude stop culture, obtain corresponding bacterium solution；Wherein solid slope culture medium：Peptone 5g/L, sodium chloride
5g/L, beef extract 5g/L, agar 1.5%-2.0%, PH are adjusted to 6.8-7.2；Fluid nutrient medium：Without agar, other compositions and contain
Amount is consistent with solid slope culture medium, and PH is adjusted to 6.5-7.5.
4）Bacillus subtilis bacterium solution, bacillus megaterium bacterium solution and colloid bacillus cereus bacterium solution are proportionally mixed
Conjunction obtains complex microorganism bacterium solution；Preferably, bacillus subtilis bacterium solution, bacillus megaterium and colloid bacillus cereus bacterium solution is mixed
It is 1 to close weight ratio：1.4：1.6；
5）By the dregs of beans of complex microorganism bacterium solution and sterilized postcooling according to mass ratio 1:10 are uniformly mixed, and low temperature drying obtains
Complex micro organism fungicide；
6）After raw material in addition to complex microorganism bacterium solution and dregs of beans is crushed respectively, mix in proportion and through low temperature,
Drying, cooling, screening are granulated, and obtain fertiliser granulates；
7）By step 5）In complex micro organism fungicide obtained and coating oil be sprayed on step 6 after mixing）In fertilizer obtained
Expect particle surface, obtains target cabbage formula fertilizer special.
Cabbage formula fertilizer special prepared by the present invention is detected with the soil organism, nutrition and microorganism species and is tied
The uptake characters of fruit and wild cabbage are foundation, and a biologic organic, inorganic formula fertilizer targetedly prepared has both microbial-bacterial fertilizer,
Organic fertilizer, the triple function of inorganic fertilizer.
In order to keep the technology in complex microorganism organic and inorganic fertilizer of the present invention definitely clear, technical advantage is more prominent
Go out, spy carries out cell manure trial, is to verify the dedicated fertilizer of the present invention for cabbage planting effect.
It tests plot and is experimental field located at the rich Mao Nongyeshengtaiyuan in Wuhan City, Hubei Province Caidian District, Wuhan subtropical zone monsoon directly under the jurisdiction of a municipal government
Property moist climate region, have that abundant rainfall, sunshine are sufficient, make a clear distinction between the four seasons, summer high temperature, precipitation are concentrated, the spies such as winter slightly cool moistening
Point.In 1 year, 1 monthly mean temperature is minimum, is 3.0 DEG C；7 monthly mean temperature highests are 29.3 DEG C, and summer is up to 135 days；Spring and autumn
Respectively about 60 days two seasons；Early summer plum rain season rainfall is relatively concentrated, and annual precipitation is 1205 millimeters.Test plot basic physical and chemical such as
Shown in table 3, experiment is ox shape head cabbage varieties for examination head cabbage varieties.It is as shown in table 3 to the analysis of physical and chemical property of soil.
3 Labile soil organic carbon of table
There is nutrient effect content Plentiful-lack index to show that this area's soil alkali-hydrolyzable nitrogen lacks by 1 soil of table, rapid available phosphorus is medium, in available potassium
Deng yield is in 4500-5500kg/ mus of wild cabbages, 13-15kg/ mus of purity nitrogen, pure phosphorus（P2O5）4-6kg/ mus, pure potassium 8-10kg/ mus, lead to
It crosses Stanford Formula and show that the area needs purity nitrogen fertilizer 14kg, pure phosphate fertilizer 4kg, pure potash fertilizer 8kg, N+P+K（14:4:8）≥26%；
This place agllutination cabbage complex microorganism organic-inorganic special fertilizer is obtained by analyzing above, and this product is former in parts by weight
Material is：60 parts of amino acid, 70 parts of humic acid, 5 parts of insect nuclear polyhedrosis virus, 40 parts of dregs of beans, 208 parts of urea, technical grade
108 parts of monoammonium phosphate, 80 parts of potassium sulfate, 0.5 part of complex microorganism bacterium solution, this formula technique index are：The quality of organic matter contains
Amount is 30%-40%, and the mass content of the sum of N, P, K three is 26%-40%, composite microbial bacteria >=0.2 hundred million/g.
3 experiments are arranged in the present embodiment, are repeated 3 times, plot area 16m2, cell is according to RANDOMIZED BLOCK DESIGN；Except fertilizer
Outside, other conditions are consistent.
Experiment 1：100kg/ mus of organic fertilizer；
Experiment 2：100kg/ mus of cabbage formula fertilizer special of the present invention；
Experiment 3：100kg/ mus of fragrant plant field 18-8-18 compound fertilizers.
Experimental result is with analysis as shown in table 4 and table 5.
Influence of 4 different fertilizer of table to wild cabbage survival rate and yield
Influence of 5 different fertilizer of table to Cabbage Quality（Per 100g wild cabbage contents）
It can be obtained by table 4,5：Experiment 2 either seedling percent or cell production and each important indicator of product quality
It will be better than experiment 1 and experiment 3, be verified by field trial, it can be deduced that such complex microorganism compound organic and inorganic fertilizer can
Nutrient in effective activation soil significantly improves the resistance of crop and the ability of disease and insect resistance, enhances crop plant cells
Vigor accelerates the growth and development of rhizobium and root system, promotes crop root flourishing；Can effective solution be currently used for cabbage
Fertilizer existing for chemical fertilizer application is excessive, specific aim is poor, nutrient is unbalanced, existing microbial manure application effect is unstable
The problems such as, the yield and quality of wild cabbage can be significantly improved after.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
With within principle, any modification, equivalent replacement, improvement and so on should all be included in the protection scope of the present invention god.
Claims (9)
1. a kind of cabbage formula fertilizer special, which is characterized in that including the following raw material in parts by weight：Amino acid 60-80
Part, 60-80 parts of humic acid, 5-10 parts of insect nuclear polyhedrosis virus, 40-60 parts of dregs of beans, 208-255 parts of urea, technical grade phosphorus
Sour monoammonium 108-174 parts, 80-110 parts of potassium sulfate, 0.5-1 parts of complex microorganism bacterium solution；In fertilizer, the mass content of organic matter
Mass content for 20%-40%, the sum of N, P, K three is 19%-40%, composite microbial bacteria >=0.2 hundred million/g.
2. a kind of cabbage formula fertilizer special as described in claim 1, which is characterized in that as follows including in parts by weight
Raw material：60 parts of amino acid, 70 parts of humic acid, 5 parts of insect nuclear polyhedrosis virus, 40 parts of dregs of beans, 208 parts of urea, technical grade phosphorus
108 parts of sour monoammonium, 80 parts of potassium sulfate, 0.5 part of complex microorganism bacterium solution；In fertilizer, the mass content of organic matter is 30%-40%,
N, the mass content of the sum of P, K three is 26%-40%, composite microbial bacteria >=0.2 hundred million/g.
3. a kind of cabbage formula fertilizer special as described in claim 1, it is characterised in that：The complex microorganism bacterium solution packet
Include bacillus subtilis bacterium solution, bacillus megaterium bacterium solution and colloid bacillus cereus bacterium solution；It is withered in the complex microorganism bacterium solution
The mass ratio of careless bacillus, bacillus megaterium and colloid bacillus cereus is 1-1.2：1-1.4：1-1.6.
4. a kind of cabbage formula fertilizer special as claimed in claim 3, it is characterised in that：In the complex microorganism bacterium solution
The mass ratio of bacillus subtilis, bacillus megaterium and colloid bacillus cereus is 1：1.4：1.6.
5. a kind of cabbage formula fertilizer special as described in claim 1, it is characterised in that：The weight of N, P, K in special fertilizer
Than for 11-20：2-10：6-16.
6. a kind of cabbage formula fertilizer special as described in claim 1, it is characterised in that：The weight of N, P, K in special fertilizer
Than being 14：4：8.
7. a kind of cabbage formula fertilizer special as described in claim 1, it is characterised in that：The complex microorganism bacterium solution with
The mass ratio of dregs of beans is 1-2：8-10.
8. a kind of preparation method of cabbage formula fertilizer special as described in claim 1-7 any one, which is characterized in that
Include the following steps：
1）Carry out the measurement of physicochemical property to the soil of cabbage planting site, including but not limited to soil alkali-hydrolyzable nitrogen, rapid available phosphorus,
Available potassium, pH, EC value, boron, calcium；
2）According to the measurement result of above-mentioned physicochemical property and cabbage breeding time regulation of fertilizer requirement, calculated by Stanford Formula
Go out the magnitude of recruitment of N, P, K needed for cabbage；
3）Bacillus subtilis bacterium solution, bacillus megaterium bacterium solution and colloid bacillus cereus bacterium solution are prepared respectively；
4）Bacillus subtilis bacterium solution, bacillus megaterium bacterium solution and colloid bacillus cereus bacterium solution are proportionally carried out mixing
To complex microorganism bacterium solution；
5）Complex microorganism bacterium solution is uniformly mixed with the dregs of beans of sterilized postcooling, low temperature drying obtains composite microbial bacteria
Agent；
6）After raw material in addition to complex microorganism bacterium solution and dregs of beans is crushed respectively, mix in proportion and through low temperature,
Drying, cooling, screening are granulated, and obtain fertiliser granulates；
7）By step 5）In complex micro organism fungicide obtained and coating oil be sprayed on step 6 after mixing）In fertilizer obtained
Expect particle surface, obtains target cabbage formula fertilizer special.
9. the preparation method of cabbage formula fertilizer special as claimed in claim 8, which is characterized in that the step 3）In it is withered
Careless bacillus bacterium solution, bacillus megaterium bacterium solution and colloid bacillus cereus bacterium solution are prepared according to following other method：
First bacterial strain is crossed access solid slope culture medium, 32 DEG C of -37 DEG C of biochemical cultivation case stationary cultures 36 hours；Use sterile water
Inclined-plane is rinsed, bacteria suspension is formed；1 volume bacteria suspension is accessed in the fluid nutrient medium of 50 volumes, 32 DEG C -37 DEG C aerobic cultures are extremely
Cell concentration is 1010A/ml orders of magnitude stop culture, obtain bacterium solution；
Wherein solid slope culture medium：Peptone 5g/L, sodium chloride 5g/L, beef extract 5g/L, agar 1.5%-2.0%, PH are adjusted to
6.8-7.2；Fluid nutrient medium：Without agar, other compositions and content are consistent with solid slope culture medium, and PH is adjusted to 6.5-7.5.
Priority Applications (1)
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Cited By (4)
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| CN110590411A (en) * | 2019-10-21 | 2019-12-20 | 武汉楚强生物科技有限公司 | Amino acid hydrolysate based on insect residues with viruses and preparation method and application thereof |
| CN112048307A (en) * | 2020-09-03 | 2020-12-08 | 甘肃省科学院生物研究所 | Soil improvement matrix for relieving cabbage planting continuous cropping obstacle and preparation method thereof |
| CN112358354A (en) * | 2020-11-09 | 2021-02-12 | 宁夏大学 | Special bud-protecting fertilizer for water-fertilizer integrated planting of day lily and preparation method thereof |
| CN116158246A (en) * | 2022-12-30 | 2023-05-26 | 宁夏农林科学院固原分院 | Fertilizing method for losing weight, enhancing efficiency, improving quality and increasing yield of cabbage |
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| CN112048307A (en) * | 2020-09-03 | 2020-12-08 | 甘肃省科学院生物研究所 | Soil improvement matrix for relieving cabbage planting continuous cropping obstacle and preparation method thereof |
| CN112358354A (en) * | 2020-11-09 | 2021-02-12 | 宁夏大学 | Special bud-protecting fertilizer for water-fertilizer integrated planting of day lily and preparation method thereof |
| CN116158246A (en) * | 2022-12-30 | 2023-05-26 | 宁夏农林科学院固原分院 | Fertilizing method for losing weight, enhancing efficiency, improving quality and increasing yield of cabbage |
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