CN1322816C

CN1322816C - Method for establishing exogenous microorganism onto plant root system

Info

Publication number: CN1322816C
Application number: CNB2005100169383A
Authority: CN
Inventors: 王忠强
Original assignee: Northeast Normal University
Current assignee: Northeast Normal University
Priority date: 2005-06-29
Filing date: 2005-06-29
Publication date: 2007-06-27
Anticipated expiration: 2025-06-29
Also published as: CN1718002A

Abstract

The present invention belongs to the application area of microorganisms, particularly to a method for establishing exogenous microorganisms onto plant root systems. Chinese low-level nutrient-rich herbaceous peat is used as main raw materials; a proper number of acidity-basicity buffering agents and chemical fertilizer are added to the main raw materials; then, the raw materials are pressed into cylinders with seed holes and cutting holes by a forming machine; sterilized plant seeds after disinfection at high temperature are planted on peat bodies in a fixing shape; then, the exogenous microorganisms are added to the peat block bodies by the injection or the pouring method; the exogenous microorganisms after being cultivated for scores of days are moved to soil. The plants are tightly combined with the microorganisms by the present invention, which is favorable to the mutual promotion of the plants and the microorganisms. The cultivated plants of the peat bodies promote the development of plant root systems, provide a protection layer for plant root regions, are favorable to enhance resistance of the plants, enhance the survival rate and the growth ability of plants under various soil environments and avoid secondary pollution. The present invention can be used for soil improvement after being used for a long time. The present invention has the advantages of simple structure and convenient operation.

Description

The method that a kind of inoculating microbe is grown surely at root system of plant

Technical field

The invention belongs to the microorganism application, be specifically related to the method that inoculating microbe is grown surely at root system of plant

Technical background

Along with the development of microbial technique, in agricultural production, aspects such as environmental pollution reparation are widely used through the inoculating microbe of domestication and improvement.Inoculating microbe grown surely in the method for root system has become agricultural produce, the important means in soil pollutant place to go has been applied to various beneficial microbe biocontrol microorganisms, the slightly living bacterium of rhizosphere, rhizobium and mycorrhizal fungi etc. the crops root system and has promoted its disease resistance, anti-adversity ability to increase yield and quality in the agricultural; Grow in soil into multiple microbial degradation place to go pedo relict agricultural chemicals such as pseudomonad, spirillum and micrococcus luteus in the environment, organic pollutants such as oil are also obtained certain effect.The method of present rhizosphere colonization external source bacterium mainly contains bacterium liquid immersion seed and grows method, root fixed-point injection method and infected water irrigation method surely.But because soil and floristics differ greatly, the variation of soil temperature, humidity and the existence of indigenous microorganism have a strong impact on the breeding of inoculating microbe, also make the action effect of inoculating microbe not obvious, and the restriction inoculating microbe is further used.

The present invention is that the microorganism of rhizosphere provides and is beneficial to survival and expands numerous suitable space and nutrients according to secretions from plant roots, be that the outer rim microorganism grows the characteristics into best region, the characteristics such as C/N ratio, pH value, porosity and fiber content that are suitable for plant and growth of microorganism that have in conjunction with China's meadow peat, peat is prepared into the combination that is suitable for plant and growth of microorganism, closely contact with inoculating microbe by root system of plant, promote microorganism to grow fast and expand numerously, make it bring into play due effect

Summary of the invention

The present invention is the method that inoculating microbe is grown surely at root system of plant, adopting China's low level eutrophy meadow peat is primary raw material, with addition of an amount of soda acid blender, chemical fertilizer, be pressed into the cylinder shape peat culture that has kind of hole and cutting hole through forming machine, through behind the high temperature sterilization plant seed of sterilizing being planted on the solid shape peat culture, then inoculating microbe injection or pouring method are added the peat block.Along with the growth of plant, root exudates increases, and inoculating microbe adapts to root system environment and breeding fast gradually, moves into soil after 10-15 days cultivation.Because inoculating microbe become the root system of plant dominant population, and along with the growth and the extension of root system of plant, the expansion of rhizosphere area and further in the plant rhizosphere environment of soil, growing surely.

The used primary raw material of the present invention is a low level eutrophy meadow peat, requires fiber content 〉=30%, water content 45%-55%, and ash content≤60%, C/N are than 25-35: 1, N/P is than being 5-10: 1.

Filler is: perlite and soda acid blender slaked lime (white lime) or calcium sulphate, the ratio of perlite and soda acid blender is about 2: 1, the adding of soda acid blender and proportioning are determined by the pH value of peat raw material, guarantee that the peat pH value is 6.5-7.5, when peat raw material pH value is satisfactory, do not add this filler.

Concrete preparation method is: the particle that with the rough pulverizing of raw material peat is 2 millimeters-10 millimeters, determine the addition of soda acid blender white lime or calcium sulphate by the pH value of measuring the peat raw material, mix more than 10 minutes at agitator with corresponding perlite addition then, enter the preparation of granules that cracker is ground into 1 millimeter and become filler, banking up after agitator mixes 20 minutes with peat enters the forming machine briquet again more than 24 hours.Not filled satisfactory peat is briquet directly.

The specification of peat culture is: profile is a cylinder, high 5-10 centimetre, and diameter 5-10 centimetre; It is conical planting the hole, and the degree of depth is 3-8 centimetre, and diameter is 1 centimetre.

Using method of the present invention: will contain the peat block that bacteria culture fluid injects high-temperature sterilization with needle tubing, and broadcast into plant seed in kind of a hole then; Also sterilization back peat body can be placed on the plane of water-impervious, 2-3 centimetre at interval of every peat body, to contain bacteria culture fluid slowly pours into, the peat block can absorb it, then plant seed is broadcast kind hole into peat body, daily management notices that temperature remains on 28-30 ℃, block water content 50-60%.Cultivated 10-15 days, treat that plant root appears at around the peat body after, transplant to soil and get final product.

The present invention has following advantage:

1 the present invention can adjust C/N ratio flexibly according to the existence characteristics of microorganism, and N/P ratio and pH value are simple and easy to use;

2 the present invention combine closely plant and microorganism, can select the collocation of suitable plant and microorganism according to growth characteristic and interdependency each other, help both mutually promoting;

The cultivation plant of 3 peat bodies promotes the root system of plant prosperity, and the district provides protective layer for plant roots, helps plant resistance to environment stress and strengthens, and improves survival rate and the growth of plant in all kinds of soil environments, the survival of indirect promotion inoculating microbe and expand numerous;

It is organic mineral resources of natural cleaning that 4 the present invention adopt peat, can not cause the soil secondary pollution, and long-term use continuously can also be improved the soil;

5 preparations are simple, can satisfy production after being used to suppress the simple remodeling of equipment of moulded coal production.

Embodiment

1 to choose fiber content be 30%, water content 45%, and ash content 60%, the C/N ratio is 25: 1, the N/P ratio is 5: 1.PH value is standby after 5 kilograms of pulverizing of meadow peat of 6.0; Need adding calcium hydroxide 14.5 grams as calculated, perlite 29 grams.The two was mixed 10 minutes at agitator, enter the preparation of granules that cracker is ground into 1 millimeter and become filler, banking up after agitator mixes 20 minutes with peat, to enter forming machine briquet profile in 24 hours be that cylindrical shape is a cylinder again, high 5 centimetres, 5 centimetres of diameters; It is conical planting the hole, and the degree of depth is 3 centimetres, and diameter is 1 centimetre.

2 to choose fiber content be 40%, water content 50%, and ash content 50%, the C/N ratio is 28: 1, the N/P ratio is 6: 1.PH value is that 5 kilograms of 6.5 meadow peats are pulverized after forming machine compacting profile is a cylinder, and is high 8 centimetres, 8 centimetres of diameters; It is conical planting the hole, and the degree of depth is 6 centimetres, and diameter is 1 centimetre.

3 to choose fiber content be 50%, water content 55%, and ash content 45%, the C/N ratio is 30: 1, the N/P ratio is 8: 1.PH value is that 5 kilograms of 7.5 meadow peats are pulverized after forming machine compacting profile is a cylinder, and is high 10 centimetres, 10 centimetres of diameters; It is conical planting the hole, and the degree of depth is 8 centimetres, and diameter is 1 centimetre.

4 to choose fiber content be 60%, water content 55%, and ash content 40%, the C/N ratio is 35: 1, the N/P ratio is 10: 1.PH value is standby after 5 kilograms of pulverizing of meadow peat of 8.0; Need add calcium sulphate 18.5 grams as calculated, perlite 37 grams.The two was mixed 10 minutes at agitator, enter the preparation of granules that cracker is ground into 1 millimeter and become filler, bank up after agitator mixes 20 minutes with peat again that to enter forming machine briquet profile in 24 hours be cylinder, high 10 centimetres, 8 centimetres of diameters; It is conical planting the hole, and the degree of depth is 8 centimetres, and diameter is 1 centimetre.To contain the peat block that bacteria culture fluid injects high-temperature sterilization with needle tubing, broadcast into plant seed in kind of a hole then; Also sterilization back peat body can be placed on the plane of water-impervious, 2-3 centimetre at interval of every peat body, to contain bacteria culture fluid slowly pours into, the peat block can absorb it, then plant seed is broadcast kind hole into peat body, daily management notices that temperature remains on 28-30 ℃, block water content 50-60%.Cultivated 10-15 days, treat that plant root appears at around the peat body after, transplant to soil and get final product.

Claims

1, the inoculating microbe method of growing surely at root system of plant, it is characterized in that: adopting China's low level eutrophy meadow peat is primary raw material, with addition of an amount of acid-base buffer agent, chemical fertilizer, be pressed into the cylinder that has kind of hole and cutting hole through forming machine, through behind the high temperature sterilization plant seed of sterilizing being planted on the solid shape peat body, then inoculating microbe injection or pouring method are added the peat block, after cultivating, move into soil;

Filler is: perlite and soda acid blender slaked lime or calcium sulphate, the ratio of perlite and soda acid blender is 2: 1, the adding of soda acid blender and proportioning are determined by the pH value of peat raw material, guarantee that the peat pH value is 6.5-7.5, do not add this filler when peat raw material pH value is satisfactory;

The preparation method is: the particle that with the rough pulverizing of raw material peat is 2 millimeters-10 millimeters, determine the addition of soda acid mediator agent slaked lime or calcium sulphate by the pH value of measuring the peat raw material, mix more than 10 minutes at agitator with corresponding perlite then, enter the preparation of granules that cracker is ground into 1 millimeter and become filler, bank up after agitator mixes 20 minutes with peat and enter the forming machine briquet more than 24 hours, not filled satisfactory peat is briquet directly;

To contain the peat block that bacteria culture fluid injects high-temperature sterilization with needle tubing, broadcast into plant seed in kind of a hole then; Also sterilization back peat body can be placed on the plane of water-impervious, 2-3 centimetre at interval of every peat body, to contain bacteria culture fluid slowly pours into, then plant seed is broadcast kind hole into peat body, daily management notices that temperature remains on 28-30 ℃, block water content 50-60% cultivated 10-15 days, transplanted to soil to get final product.

2, the method for growing surely at root system of plant by the inoculating microbe of claim 1 is characterized in that primary raw material low level eutrophy meadow peat, fiber content 〉=30%, and water content 45%-55%, ash content≤60%, C/N are than 25-35: 1, N/P is than being 5-10: 1.

3, the method for growing surely at root system of plant by the inoculating microbe of claim 1 is characterized in that the specification of peat culture is: profile is a cylinder, high 5-10 centimetre, and diameter 5-10 centimetre; It is conical planting the hole, and the degree of depth is 3-8 centimetre, and diameter is 1 centimetre.