CN102503666B - Horticultural crop composite biological nutrient medium and preparation method thereof - Google Patents

Horticultural crop composite biological nutrient medium and preparation method thereof Download PDF

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Publication number
CN102503666B
CN102503666B CN 201110321771 CN201110321771A CN102503666B CN 102503666 B CN102503666 B CN 102503666B CN 201110321771 CN201110321771 CN 201110321771 CN 201110321771 A CN201110321771 A CN 201110321771A CN 102503666 B CN102503666 B CN 102503666B
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China
Prior art keywords
soil
composite biological
chlorella
nutrient medium
matrix
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CN 201110321771
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Chinese (zh)
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CN102503666A (en
Inventor
李赟
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中国海洋大学
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Priority to CN 201110321771 priority Critical patent/CN102503666B/en
Publication of CN102503666A publication Critical patent/CN102503666A/en
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Abstract

The invention relates to a horticultural crop composite biological nutrient medium which comprises a substrate, and microalgae and a culture fluid thereof. The horticultural crop composite biological nutrient medium is characterized by comprising the following components in percentage by mass: 1.5-5.5% of sodium alginate, 0.5-3.5% of chitosan, 0.5-5.5% of polyacrylamide, 1-5% of sodium carboxymethyl cellulose, 5-20% of shell powder and 60.5-91.5% of clay. The microalgae are fresh water Chlorella which can be autotrophic, heterotrophic or mixedly cultured after being cultured to later exponential growth phase, the concentration is greater than or equal to 3.0*10<6>cell/ml, and the addition amount is preferably sufficient in the substrate without flowing. The invention has the effects of moisture retention, nutrient slow release and soil improvement; most materials are aquiculture byproducts, thereby changing wastes into valuable substances and having obvious environmental protection effect; and the nutrient medium can be completely degraded within 3-5 years, and can not have permanent damage on soil. The microalgae reserved in the soil can have an active effect on the soil, increase the soil fertility and improve the physical and chemical properties of the soil.

Description

A kind of horticultural crop composite biological nutrient medium and preparation method thereof
Technical field
The present invention relates to a kind of for promoting composite biological nutrient base that economic plants grows and preparation method thereof.
Background technology
The seed rearing of the contour economic worth plant of gardening is very important link, and good seed quality determines the quality of vine growth and development, and final directly related with economic yield.Just because of this, seed rearing has now adopted the proprietary culture facilities such as seedbed, nutrition pot to carry out.The factor that determines the seedling quality quality is a lot, is the nutrition base that mixes formation be used to the matrix of growing seedlings and with matrix and wherein be affected by human factors maximum.
It is formulated that the nutrition Quito that is used at present seed rearing is that the fertilizer by fermentation is aided with turfy soil.This nutrition base Main Problems is: moisture holding capacity is poor, and the nutrition base hardens easily, and air permeability is poor, is unfavorable for seedling development.
Summary of the invention
For the problems referred to above, one of purpose of the present invention provides a kind of horticultural crop composite biological nutrient medium with water conservation, nutrition slow-release, reclamation result, to satisfy the needs of high economic worth crop seedling and one-tenth bulk-growth.
Another object of the present invention provides the preparation method of above-mentioned nutrition base.
The objective of the invention is to be achieved through the following technical solutions: will grow to chlorella and nutrient solution and water-holding agent and the oyster shell whiting combination of exponential phase, the promoted plant root growth that utilizes chlorella to produce on the one hand, but another fermentation utilizes the ability of chlorella heterotrophism or mixed culture, keep its lasting biologos, and, can play with water-holding agent and oyster shell whiting acting in conjunction based on chlorella and secretory substance thereof and to improve soil physical and chemical property, promote the effect of the formation of soil aggregate; Simultaneously, utilize the crosslinked action of water-holding agent and oyster shell whiting, form the nutritive cube with definite shape, be convenient to practical application.
For reaching above-mentioned purpose, concrete technical scheme of the present invention is as follows:
A kind of horticultural crop composite biological nutrient medium comprises matrix and little algae and nutrient solution thereof, it is characterized in that described matrix comprises following mass component:
Sodium alginate: 1.5-5.5%;
Chitin: 0.5-3.5%;
Polyacrylamide: 0.5-5.5%;
Xylo-Mucine: 1-5%;
Oyster shell whiting: 5-20%;
Clay: 60.5-91.5%;
But described little algae is the limnetic chlorella of the autotrophy, heterotrophism and the mixed culture that are cultured to the exponential growth later stage, its concentration 〉=3.0 * 10 6Cell/ml, consumption do not trickle to pour into sufficient in the described matrix and are advisable.
The nutrient solution of described limnetic chlorella is the BG-1 substratum, and this medium component is respectively: NaNO 31.5g, K 2HPO 40.04g, MgSO 4.7H 2O 0.075g, CaCl 2.7H 2O 0.036g, Na 2CO 30.02g, citric acid 0.006g, ironic citrate 0.006g, trace element solution 1ml, distilled water 1000ml.
Described trace element solution composition is H 3BO 42.86g, MnCl 2.4 H 2O 1.81g, ZnSO 40.222g, Na 2MoO 40.39g, CuSO 4.5 H 2O 0.079g, Co (NO 3) 2.6 H 2O 49.4g is dissolved in the 1000ml distilled water.
The preparation method of above-mentioned composite biological nutrient base: determine the consumption of oyster shell whiting to add successively subsequently sodium alginate, chitin, Xylo-Mucine and polyacrylamide according to aforementioned proportion; After said components mixed, mix with the clay of filling up again, fully stir, be contained in as early as possible in the container that is added with liner (being generally old and useless newspaper or thieving paper etc.) compacting; Chlorella and nutrient solution thereof are poured in the above-mentioned matrix that mixes, consumption does not trickle with abundance and is advisable again; Placed 24 hours at shady and cool dry place, treat that nutrition primary surface moisture slightly has evaporation to use.
The invention has the beneficial effects as follows: chitin has anti-microbial activity, and sodium alginate can be combined with chitin and calcium salt and be formed cotton-shaped gel precipitation, and polyacrylamide and Xylo-Mucine are powerful water-holding agents, and common the use guaranteed lasting water-retentivity.The oyster shell whiting main component is calcium carbonate, is porousness, has good adsorptivity, is combined with above-mentioned four kinds of materials can play the absorption nutritive ingredient and the slowly effect of release.Cultivate that chlorella self contains or the various active materials such as towards periphery medium secretion amino acid, carbohydrate, VITAMIN, plant hormone, can keep for a long time validity.The macromolecular substance and nitrogen, phosphorus, potassium macroelement and the chitin three that secrete to the micro algae culturing liquid form the nutrition protective membrane jointly; neither affect Crop; can stop again the intrusion of extraneous germ, reduce disease and pest, guarantee that to greatest extent nutritive element is by plant absorbing simultaneously.Therefore, this nutrition base is combined in the aspects such as water conservation, nutrition slow-release, biologically active substance, and can to continue to supply growing of plant each period required, can improve soil physical and chemical property, promotes the formation of soil aggregate.Make nutritive cube, can conveniently be used for fruit tree, vegetables and flowers seed rearing, also can conveniently be used for the nutrition supply of bearing tree body.
The used most of material of the present invention is the aquaculture byproduct, can turn waste into wealth, and has obvious environmental protection effect; Made nutrition base can all be degraded in 3-5, can not cause nonvolatil injury to soil.The little algae that remains in the soil can produce lasting positive influence to soil, increases soil fertility, improves its physicochemical character.
Embodiment
The present invention is described in detail below by specific embodiment.
Embodiment 1:
The matrix of horticultural crop composite biological nutrient medium is comprised of following mass component:
Sodium alginate: 1.5%;
Chitin: 1.0%;
Polyacrylamide: 2.0%;
Xylo-Mucine: 1.5%;
Oyster shell whiting: 15%;
Clay: 79.0%.
Adopt the BG-1 substratum to cultivate through autotrophy, heterotrophism in bioreactor as nutrient solution and raise together with the chlorella of screening, the chlorella of this screening is cultured to the exponential growth later stage, its concentration can reach 3.0 * 10 7Cell/ml.
The preparation method of above-mentioned composite biological nutrient base: according to aforementioned proportion, determine the consumption of oyster shell whiting, add successively subsequently sodium alginate, chitin, Xylo-Mucine and polyacrylamide; After said components mixed, mix with the clay of filling up again, fully stir, be contained in as early as possible in the container that is added with liner (being generally old and useless newspaper or thieving paper etc.) compacting; Chlorella and nutrient solution thereof are poured in the above-mentioned matrix that mixes, consumption does not trickle with abundance and is advisable again; Placed 24 hours at shady and cool dry place, treat that nutrition primary surface moisture slightly has evaporation to use.
The water retention property of this nutrition base improves 30% than nutrition pot commonly used, and (20 days) can reduce and pour water 1 time between nursery stage.It is 92% that cultivation seedling transplanting survival rate reaches the contrast of 100%(nutrition pot).
Embodiment 2:
The matrix of horticultural crop composite biological nutrient medium is comprised of following mass component:
Sodium alginate: 5.0%;
Chitin: 0.5%;
Polyacrylamide: 3.0%;
Xylo-Mucine: 2.5%;
Oyster shell whiting: 20%;
Clay: 69%.
Adopt the BG-1 substratum to cultivate chlorella to the exponential growth later stage as nutrient solution, chlorella concentration reaches 3.0 * 10 6More than the cell/ml.
The preparation method of above-mentioned composite biological nutrient base: according to aforementioned proportion, determine the consumption of oyster shell whiting, add successively subsequently sodium alginate, chitin, Xylo-Mucine and polyacrylamide; After said components mixed, mix with the clay of filling up again, fully stir, be contained in as early as possible in the container that is added with liner (being generally old and useless newspaper or thieving paper etc.) compacting; Chlorella and nutrient solution thereof are poured in the above-mentioned matrix that mixes, consumption does not trickle with abundance and is advisable again; Placed 24 hours at shady and cool dry place, treat that nutrition primary surface moisture slightly has evaporation to use.This nutritive cube can be bestowed 20cm under the peripheral soil layer of the top fruit sprayer of growing up, and plays water conservation, fertilizer conservation, the effect of slowly-releasing.
Embodiment 3:
The matrix of horticultural crop composite biological nutrient medium is comprised of following mass component:
Sodium alginate: 5.5%;
Chitin: 3.5%;
Polyacrylamide: 5.5%;
Xylo-Mucine: 5%;
Oyster shell whiting: 20%;
Clay: 60.5%;
Adopt the BG-1 substratum to cultivate chlorella to the exponential growth later stage as nutrient solution, chlorella concentration reaches 3.0 * 10 6More than the cell/ml.
The preparation method of above-mentioned composite biological nutrient base: according to aforementioned proportion, determine the consumption of oyster shell whiting, add successively subsequently sodium alginate, chitin, Xylo-Mucine and polyacrylamide; After said components mixed, again with the clay mixing; The matrix that more than becomes the nutrition base be grouped into is bestowed soil 20cm place by 20% mass percent and mixing with soil.Fertilising place is watered to contain chlorella concentration and is reached 3.0 * 10 6The above nutrient solution of cell/ml (quality of nutrient solution be about apply nutrition based substrate quality 30%).

Claims (3)

1. a horticultural crop composite biological nutrient medium comprises matrix and little algae and nutrient solution thereof, it is characterized in that described matrix comprises following mass component:
Sodium alginate: 1.5-5.5%;
Chitin: 0.5-3.5%;
Polyacrylamide: 0.5-5.5%;
Xylo-Mucine: 1-5%;
Oyster shell whiting: 5-20%;
Clay: 60.5-91.5%;
But described little algae is the limnetic chlorella of the autotrophy, heterotrophism and the mixed culture that are cultured to the exponential growth later stage, its concentration 〉=3.0 * 10 6Cell/ml, consumption pour in the described matrix sufficient with chlorella and nutrient solution thereof and do not trickle and be advisable.
2. composite biological nutrient base according to claim 1, the nutrient solution that it is characterized in that described limnetic chlorella is the BG-1 substratum, this medium component is respectively: NaNO 31.5g, K 2HPO 40.04g, MgSO 4.7H 2O 0.075g, CaCl 2.7H 2O 0.036g, Na 2CO 30.02g, citric acid 0.006g, ironic citrate 0.006g, trace element solution 1ml, distilled water 1000ml; Described trace element solution composition is H 3BO 42.86g, MnCl 2.4 H 2O 1.81g, ZnSO 40.222g, Na 2MoO 40.39g, CuSO 4.5 H 2O 0.079g, Co (NO 3) 2.6 H 2O 49.4g is dissolved in the 1000ml distilled water.
3. the preparation method of composite biological nutrient base according to claim 1, it is characterized in that may further comprise the steps: according to the consumption of the described ratio-dependent oyster shell whiting of claim 1, add successively subsequently sodium alginate, chitin, Xylo-Mucine and polyacrylamide; After said components mixed, mix with the clay of filling up again, fully stir, be contained in as early as possible in the container that is added with liner compacting; Chlorella and nutrient solution thereof are poured in the above-mentioned matrix that mixes, consumption does not trickle with abundance and is advisable again; Placed 24 hours at shady and cool dry place, treat that nutrition primary surface moisture slightly has evaporation to use.
CN 201110321771 2011-10-21 2011-10-21 Horticultural crop composite biological nutrient medium and preparation method thereof CN102503666B (en)

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CN102992884A (en) * 2012-10-26 2013-03-27 张建新 Preparation method for novel nutrient soil
CN102992883A (en) * 2012-10-26 2013-03-27 张建新 Novel nutrient soil
CN103011974A (en) * 2012-12-26 2013-04-03 福建超大集团有限公司 Manufacture method of chitin biological fertilizer with slow-release function
CN104263376B (en) * 2014-09-22 2017-11-21 安徽大泽生物肥料科技有限公司 A kind of soil compound water retaining agent and its production and use
CN106106086A (en) * 2016-06-28 2016-11-16 阜阳市殿兴农业科技有限公司 A kind of drought-hit area cultivated strawberry substrate
CN106866211A (en) * 2017-03-09 2017-06-20 郑金秀 A kind of interior ornamental plant soil-less culturing nutrient material and preparation method thereof
CN106810379A (en) * 2017-03-14 2017-06-09 山东省联合农药工业有限公司 A kind of pepper seedling raising medium and preparation method and the method that pepper seedling raising is carried out using the matrix
CN109006432A (en) * 2018-08-01 2018-12-18 李慧 Application of the chlorella in cultivation technique without soil
CN109845609A (en) * 2019-04-12 2019-06-07 江苏里下河地区农业科学研究所 A kind of household balcony Chunlan cultural method
CN110079334A (en) * 2019-06-15 2019-08-02 苏州逸纪杰电子科技有限公司 A kind of dedicated renovation agent of salt-affected soil and preparation method thereof

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CN1539266A (en) * 2003-11-04 2004-10-27 北京千年美能科技有限责任公司 Ground substance with whole nourishment for cultivating seeding from tissue
CN1702061A (en) * 2004-05-24 2005-11-30 北京市大东流苗圃 High-activity plant culturing nutrient matrix and method for making same
CN1322816C (en) * 2005-06-29 2007-06-27 东北师范大学 Method for establishing exogenous microorganism onto plant root system
CN101429062B (en) * 2007-11-07 2011-05-25 浙江省林业科学研究院 Multifunctional additive for greening seedling growth substrate
CN102173917B (en) * 2011-01-06 2013-03-27 四川大学 Preparation method of afforestation matrices for sandstone rock slopes

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