CN115777489B - Garden engineering biological greening method - Google Patents
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Abstract
The invention belongs to the field of landscaping, and provides a biological greening method for garden engineering, which mainly comprises preparation technologies of a microbial composite microbial agent suitable for improving raw soil, root surrounding soil, growth soil and the like. The method can effectively improve the raw soil, improve the microbial community structure of the raw soil, increase the aggregate structure of the raw soil, improve the planting survival rate of the seedlings for greening the raw soil and promote the growth of the seedlings for greening the garden of the raw soil.
Description
Technical Field
The invention belongs to the field of landscaping, and particularly relates to a biological greening method for garden engineering.
Background
The disclosure of this background section is only intended to increase the understanding of the general background of the invention and is not necessarily to be construed as an admission or any form of suggestion that this information forms the prior art already known to those of ordinary skill in the art.
Along with the continuous progress of society, people's requirements for living environment are continuously promoted, and many greening projects are in relatively barren areas such as by roads, abandoned mines, landfill sites, river ditches and the like, and the areas are not cultivated, and the soil is mostly raw soil, so that the greening difficulty is high. Raw soil refers to soil which is not cultivated and cured, and is generally firm in soil quality, low in organic matter content, poor in soil aggregate structure, low in water absorption and fertilizer retention performance, poor in physical and chemical properties, weak in microbial activity, unsuitable for normal growth of crops, and required to be improved to become mature soil before being suitable for cultivation. The raw soil can be utilized only after being modified or cured. At present, landscaping under a raw soil environment mostly adopts a soil-alien method, namely, raw soil is covered with delicately soil. The method comprises the steps of transporting the mature soil in the farmland to a raw soil area needing greening, covering the well-flattened raw soil with the mature soil, achieving the purpose of improving the raw soil, and then greening and the like. The method has high transportation cost and large workload, and if the amount of the mature soil is insufficient, the growth restriction of the greening seedlings in the later period is larger.
The improvement of the raw soil and the utilization of the in-situ raw soil as much as possible are effective methods for landscaping engineering in these areas. At present, the soil improvement mainly adopts measures such as in-situ sand paving, soil improvement agent application, organic improvement materials such as organic fertilizer and the like, and can play a certain role in soil improvement. For example, a raw soil curing formula and method (patent number: 202110572830 1) are disclosed, which adopts raw soil, yellow sand, turf, organic fertilizer, coconut husk, microbial inoculant and soil conditioner to carry out raw soil improvement, and a curing agent and application thereof in promoting raw soil curing of a mining area dumping site (patent number: 2021102297101) are disclosed, wherein the curing agent comprises trichoderma viride (Trichoderma viride) and gulonic acid mother liquor, and has remarkable curing effect on raw soil of the mining area dumping site. However, the existing soil improvement method or formula has poor pertinence, and cannot be directly applied to the landscaping work of raw soil.
Disclosure of Invention
In order to solve the problems, the invention aims to provide a method for biologically improving the soil of landscaping engineering, which mainly comprises the preparation technology of a microbial composite microbial agent, root surrounding soil, growth soil and the like suitable for improving the soil. The method can effectively improve the raw soil, improve the microbial community structure of the raw soil, increase the aggregate structure of the raw soil, improve the planting survival rate of the seedlings for greening the raw soil and promote the growth of the seedlings for greening the garden of the raw soil.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
in a first aspect of the present invention, there is provided a method for greening a garden engineering organism, comprising:
turf, wormcast and weathered coal are mixed according to a volume ratio of 5: 1-2: mixing the materials according to the proportion of 0.2 to 0.3 to form the rhizobia agent adsorption carrier;
bacillus subtilis Bacillus subtilis GE2: bacillus aryasis Bacillus aryabhattai BPR088 and lactobacillus casei according to viable count 4:2 to 4: mixing the materials in a proportion of 0.2 to 0.5 to form a rhizosphere liquid microbial composite microbial agent;
inoculating the rhizosphere liquid composite microbial agent into a rhizosphere microbial agent adsorption carrier according to the proportion of 5% -6% mL/g to form a rhizosphere solid microbial agent;
the method comprises the steps of (1) mixing raw soil, a root-surrounding solid microbial inoculum, turf and decomposed chicken manure according to a volume ratio of 5:1 to 1.5: 3-4: 1-2, and forming root surrounding soil;
in addition, the decomposed chicken manure, wormcast and weathered coal are mixed according to the volume ratio of 5: 2-3: mixing in the ratio of 0.5-1 to form a growing microbial agent adsorption carrier;
bacillus aryasis Bacillus aryabhattai BPR088 and lactobacillus casei according to viable count 4: 3-4 to form a growth liquid microorganism composite microbial agent;
inoculating the growth liquid microorganism composite microbial inoculum into a growth microbial inoculum adsorption carrier according to the proportion of 6% -10% mL/g to form a growth solid microbial inoculum;
raw soil is prepared by the steps of: organic fertilizer: the solid microbial inoculum grows according to the volume ratio of 5: 3-4: uniformly mixing the materials in a proportion of 0.5 to 1, and then adding a graft copolymerization humic acid and bentonite super absorbent composite material with a weight ratio of 0.3 to 0.6 per mill to form growth soil;
when planting the greening seedlings, surrounding the root systems of the seedlings by using non-woven fabrics, filling root surrounding soil in the non-woven fabrics, filling growth soil between the non-woven fabrics and tree holes, planting the greening seedlings, and watering and managing to obtain the greening seedlings.
Aiming at the current situation of improving the raw soil, the invention provides a method for biologically improving the raw soil in the landscaping engineering.
In a second aspect, the invention provides an application of the method in landscaping with earth.
The beneficial effects of the invention are that
(1) The invention utilizes biological method to improve raw soil for landscaping, and aims at survival and later growth of greening seedlings, and the greening seedlings are backfilled by root surrounding soil and growth soil, thereby reducing transportation cost of alien soil and greatly reducing labor.
(2) The rhizosphere liquid microbial composite microbial agent with the bacillus subtilis GE2 and the bacillus aryaensis BPR088 as main components is suitable for barren soil environment, can survive in the soil environment, is beneficial to rooting of greening seedlings and improves the survival rate.
(3) The growth liquid microorganism composite microbial agent taking the bacillus aryabhattai BPR088 and the lactobacillus casei as main modifiers can improve the microbial community structure of raw soil, improve the aggregate structure of the soil, improve the preservation rate of greening seedlings and promote the growth of the seedlings.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention.
FIG. 1 is a schematic diagram of the invention for planting green seedlings.
Detailed Description
It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the invention. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
A garden engineering biological greening method, comprising:
turf: wormcast: weathered coal according to the volume ratio of 5: 1-2: mixing the materials in a proportion of 0.2 to 0.3 to form the rhizobia agent adsorption carrier.
Bacillus subtilis (Bacillus subtilis) GE2: bacillus aryasis (Bacillus aryabhattai) BPR088 and lactobacillus casei according to viable count=4: 2 to 4: mixing the materials in a proportion of 0.2 to 0.5 to form the root canal liquid microbial composite microbial agent.
The bacillus subtilis (Bacillus subtilis) GE2 is preserved in China general microbiological culture Collection center (address: north Chen West Lu No.1, 3 of the Korean area of Beijing, china) for 1 month and 7 days in 2016, and the preservation number is CGMCC No.11965.
The Bacillus aryasis (Bacillus aryabhattai) BPR088 is preserved in China general microbiological culture Collection center (address: north Chen West Lu No.1, 3 of the Korean area of Beijing, china) with a preservation number of CGMCC No.11972 in the 1 st 7 th year of 2016.
The lactobacillus casei (Lactobacillus casei) used in the invention is derived from China general microbiological culture collection center (CGMCC), and the strain number is 2.0666. Commercially available, their culture and growth conditions are carried out according to instructions provided by the culture collection unit.
Inoculating the rhizosphere liquid compound microbial agent into a rhizosphere microbial agent adsorption carrier according to the proportion of 5% -6% (mL/g) to form the rhizosphere solid microbial agent.
According to raw soil: root canal solid microbial inoculum: turf: rotten chicken manure = 5:1 to 1.5: 3-4: mixing evenly in a proportion of 1-2 to form the root surrounding soil (volume ratio).
Decomposing chicken manure: wormcast: weathered coal according to the volume ratio of 5: 2-3: mixing in the ratio of 0.5-1 to form the adsorbent carrier for the growth microbial inoculum.
Bacillus aryasis (Bacillus aryabhattai) BPR088 and lactobacillus casei according to viable count=4: 3-4 to form the growth liquid microorganism composite microbial agent.
Inoculating the growth liquid composite microbial agent into a growth microbial agent adsorption carrier according to the proportion of 6% -10% (mL/g) to form a growth solid microbial agent.
According to raw soil: organic fertilizer: growth solid microbial agent = 5: 3-4: mixing evenly in a proportion of 0.5 to 1, and then adding graft copolymerization humic acid and bentonite super absorbent composite material with a weight ratio of 0.3 per mill to 0.6 per mill to form the growth soil.
The graft copolymerization humic acid and bentonite super absorbent composite material is prepared by a patent 'graft copolymerization humic acid and bentonite super absorbent composite material and a manufacturing method thereof' (patent number: 2007101145506).
When planting the garden greening nursery stock, dig circular tree pit, its height and degree of depth are according to transplanting the size of nursery stock and decide. The raw soil in the tree pit is preferably dug out more than half a year in advance, and is fully aired and turned over for a plurality of times during the period. When planting the greening seedlings, enclosing the root system of the seedlings by using degradable non-woven fabrics, filling root enclosing soil in the non-woven fabrics, filling growth soil between the non-woven fabrics and tree pits, planting the greening seedlings according to a conventional method, and watering and managing.
The invention will now be described in further detail with reference to the following specific examples, which should be construed as illustrative rather than limiting.
EXAMPLE 1 Effect of root and growth soil
The preparation method of the root surrounding soil comprises the following steps: turf: wormcast: weathered coal according to the volume ratio of 5:1.5: mixing in the ratio of 0.2 to form the rhizobia agent adsorption carrier. Bacillus subtilis GE2: bacillus aryasis BPR088 and lactobacillus casei according to viable count=4: 2: mixing in a proportion of 0.5 to form the rhizosphere liquid microbial composite microbial agent. Inoculating the rhizosphere liquid compound microbial agent into a rhizosphere microbial agent adsorption carrier according to the proportion of 5% (mL/g) to form the rhizosphere solid microbial agent. According to raw soil: root canal solid microbial inoculum: turf: rotten chicken manure volume ratio = 5:1.5:3:1, and uniformly mixing to form the root surrounding soil.
The preparation method of the growth soil comprises the following steps:
decomposing chicken manure: wormcast: weathered coal according to the volume ratio of 5:3: mixing in a proportion of 0.5 to form a growing microbial agent adsorption carrier; bacillus aryasis BPR088 and lactobacillus casei according to viable count=4: 4, mixing in proportion to form a growth liquid microorganism composite microbial agent; inoculating the growth liquid composite microbial agent into a growth microbial agent adsorption carrier according to the proportion of 10% (mL/g) to form the growth solid microbial agent. According to raw soil: organic fertilizer: growth solid microbial agent = 5:4: mixing evenly in a proportion of 0.5, and then adding graft copolymerization humic acid and bentonite super absorbent composite material with a weight ratio of 0.3 per mill to form the growth soil.
Three treatments were set up using potting experiments, one for each of the plants as (1) experimental group: according to the technical scheme of the invention, root surrounding soil and growth soil are respectively filled; (2) control group 1: the root surrounding soil and the growth soil are not separated during planting, and the root surrounding soil is added; (3) control group 2: the planting is carried out without dividing root surrounding soil and growth soil, and the growth soil is added. The experimental soil for potting comes from the raw soil on both sides of Wen river in Jinan province, the seedlings adopt white wax seedlings, the white wax seedlings with basically consistent growth vigor are selected for transplanting in the period of 31 days of 4 months in 2021, the pot height is 25cm, the pot width is 36cm, 30 pots are treated each time, 3 times are repeated, the survival rate in the current year is calculated, the plant height and the ground diameter are measured, and the next year preservation rate is calculated in the period of 26 days of 8 months in 2022. As can be seen from Table 1, the invention can remarkably improve the survival rate and the next year preservation rate of the white wax nursery stock and remarkably promote the growth of the plant height and the ground diameter of the nursery stock.
TABLE 1 survival and growth of different treatments of white waxes
EXAMPLE 2 action of the Strain of the present invention
The preparation method of the root surrounding soil and the growth soil of the experimental group comprises the following steps:
turf: wormcast: weathered coal according to the volume ratio of 5:2: mixing in the ratio of 0.3 to form the rhizobia agent adsorption carrier. Bacillus subtilis GE2: bacillus aryasis BPR088 and lactobacillus casei according to viable count=4: 4: mixing in the ratio of 0.2 to form the rhizosphere liquid microbial composite microbial agent. Inoculating the rhizosphere liquid compound microbial agent into a rhizosphere microbial agent adsorption carrier according to the proportion of 5% (mL/g) to form the rhizosphere solid microbial agent. According to raw soil: root canal solid microbial inoculum: turf: rotten chicken manure volume ratio = 5:1:3:2, uniformly mixing to form the root surrounding soil.
Decomposing chicken manure: wormcast: weathered coal according to the volume ratio of 5:2: mixing in the ratio of 0.8 to form a growing microbial agent adsorption carrier; bacillus aryasis BPR088 and lactobacillus casei according to viable count=4: 4, mixing in proportion to form a growth liquid microorganism composite microbial agent; inoculating the growth liquid composite microbial agent into a growth microbial agent adsorption carrier according to the proportion of 8% (mL/g) to form a growth solid microbial agent. According to raw soil: organic fertilizer: growth solid microbial agent = 5:3:1, and then adding 0.5 per mill of graft copolymerization humic acid and bentonite super absorbent composite material to form the growth soil.
The preparation method of the root surrounding soil and the growth soil of the control group 1 comprises the following steps:
bacillus subtilis GE2 was replaced with Bacillus subtilis GE1, bacillus aryabhattai BPR088 was replaced with Bacillus aryabhattai JIA2, and the root canal soil and the growth soil were prepared in the same manner as in treatment 1.
Bacillus subtilis GE1 and bacillus aryaensis JIA2 are strains screened by the inventor and are preserved in the common microorganism center of China Committee for culture Collection of microorganisms (address: north Chen West Lu No.1, 3 of the Korean area of Beijing, china), and the preservation numbers are CGMCC No.11964 and CGMCC No.11971, and the preparation method of the root soil and the growth soil of the control group 2 comprises the following steps:
bacillus subtilis GE2 and Bacillus aryabhattai BPR088 were replaced with Bacillus fusiformis L106, and the root canal soil and the growth soil were prepared in the same manner as in treatment 1.
Bacillus fusiformis L106 is a strain screened by the inventor and is preserved in China general microbiological culture Collection center (address: north Chen West Lu No.1, no. 3 in the Korean region of Beijing city, china) with a preservation number of CGMCC No.7666.
And (3) adopting a potting experiment, wherein the potting experiment soil is from the soil on both sides of the Wenshen in the south of the Ji, and the seedlings adopt white wax seedlings. White wax seedlings with basically consistent growth vigor are selected for transplanting at the time of 30 days of 4 months of 2021, and the pot height is 25cm and the pot width is 36cm. During transplanting, adding root surrounding soil and growing according to experimental designs related to respective treatments, repeating for 3 times for 30 pots per treatment, calculating the survival rate in the same year, and measuring plant height and ground diameter. As can be seen from Table 2, compared with the control group 1 and the control group 2, the technical scheme of the invention can obviously improve the survival rate of the white wax seedlings and obviously promote the growth of the plant height and the ground diameter of the seedlings.
TABLE 2 survival and growth of different treatments of white waxes
EXAMPLE 3 microbial agent adsorption Carrier
The preparation method of the root surrounding soil and the growth soil of the experimental group comprises the following steps:
turf: wormcast: weathered coal according to the volume ratio of 5:1: mixing in the ratio of 0.2 to form the rhizobia agent adsorption carrier. Bacillus subtilis GE2: bacillus aryasis BPR088 and lactobacillus casei according to viable count=4: 3: mixing in a proportion of 0.5 to form the rhizosphere liquid microbial composite microbial agent. Inoculating the rhizosphere liquid compound microbial agent into a rhizosphere microbial agent adsorption carrier according to the proportion of 5% (mL/g) to form the rhizosphere solid microbial agent. According to raw soil: root canal solid microbial inoculum: turf: rotten chicken manure volume ratio = 5:1.5:3:2, uniformly mixing to form the root surrounding soil.
Decomposing chicken manure: wormcast: weathered coal according to the volume ratio of 5:3: mixing in the ratio of 0.8 to form a growing microbial agent adsorption carrier; bacillus aryasis BPR088 and lactobacillus casei according to viable count=4: 3, mixing in proportion to form a growth liquid microorganism composite microbial agent; inoculating the growth liquid composite microbial agent into a growth microbial agent adsorption carrier according to the proportion of 10% (mL/g) to form the growth solid microbial agent. According to raw soil: organic fertilizer: growth solid microbial agent = 5:4:1, and then adding 0.3 per mill of graft copolymerization humic acid and bentonite super absorbent composite material to form the growth soil.
The preparation method of the root surrounding soil and the growth soil of the control group comprises the following steps:
and replacing the rhizosphere microbial agent adsorption carrier and the growth microbial agent adsorption carrier of the experimental group with turf, and only preparing rhizosphere soil and growth soil according to the same steps.
And (3) arranging greening experiments on the bases of comprehensive treatment projects on two sides of the Wen river in the great south China, wherein the seedlings adopt shrubalthea. Transplanting is carried out at the period of 26 days 3 and 3 in 2022, the root surrounding soil and the growth soil are filled according to the experimental design, the survival rate is investigated at the period of 9 months 2022, and the bacterial quantity in the root surrounding soil of the experimental group and the control group is measured. The results showed that the bacterial count of the experimental group was 5.1X10 5 CFU/g, the number of bacteria in the control group was only 5.7X10 4 The survival rate of the CFU/g of the experimental group reaches 92.4 percent, and the survival rate of the control group is 83.7 percent, so that the carrier is considered to be beneficial to the growth and propagation of microorganisms, further the number of the microorganisms is increased, the effect is better exerted, and the survival rate of the seedlings is increased.
The above description is only of the preferred embodiments of the present invention and is not intended to limit the present invention, but various modifications and variations can be made to the present invention by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (7)
1. A garden engineering biological greening method, which is characterized by comprising the following steps:
turf, wormcast and weathered coal are mixed according to a volume ratio of 5: 1-2: mixing the materials according to a proportion of 0.2-0.3 to form a rhizobia agent adsorption carrier;
bacillus subtilis is preparedBacillus subtilisGE2: bacillus aryabhattaiBacillus aryabhattai BPR088 and Lactobacillus caseiLactobacilluscaseiAccording to the viable count of 4: 2-4: mixing the materials in a proportion of 0.2-0.5 to form a root canal liquid microorganism composite microbial agent;
the bacillus subtilisBacillus subtilisGE2, which has been preserved in the China general microbiological culture Collection center of China Committee for culture Collection of microorganisms for 1 month and 7 days, has a preservation number of CGMCC No.11965, and is designated as Hospital No. 3 in North Chen West Lu of Kogyang area of Beijing, china;
the bacillus aryabhattaiBacillus aryabhattaiBPR088 is preserved in China general microbiological culture Collection center (China general microbiological culture Collection center) for 1 month and 7 days in 2016, and has a preservation number of CGMCC No.11972, namely, no. 3 of North Chen West Lu 1, the Korean region of Beijing, china;
the lactobacillus caseiLactobacilluscaseiIs derived from China general microbiological culture Collection center (CGMCC) with a preservation number of CGMCC No.2.0666;
inoculating the rhizosphere liquid composite microbial agent into a rhizosphere microbial agent adsorption carrier according to the proportion of 5% -6%, so as to form a rhizosphere solid microbial agent;
the method comprises the steps of (1) mixing raw soil, a root-surrounding solid microbial inoculum, turf and decomposed chicken manure according to a volume ratio of 5: 1-1.5: 3-4: mixing uniformly in a proportion of 1-2 to form root surrounding soil;
in addition, the decomposed chicken manure, wormcast and weathered coal are mixed according to the volume ratio of 5: 2-3: mixing in a proportion of 0.5-1 to form a growing microbial inoculum adsorption carrier;
bacillus aryabhattaiBacillus aryabhattaiBPR088 and lactobacillus casei according to viable count 4: 3-4, and forming a growth liquid microorganism composite microbial agent;
inoculating the growth liquid microorganism composite microbial inoculum into a growth microbial inoculum adsorption carrier according to the proportion of 6% -10%, so as to form a growth solid microbial inoculum;
raw soil is prepared by the steps of: organic fertilizer: the solid microbial inoculum grows according to the volume ratio of 5: 3-4: uniformly mixing the materials in a proportion of 0.5 to 1, and then adding a graft copolymerization humic acid and bentonite super absorbent composite material in a weight ratio of 0.3 to 0.6 per mill to form growth soil;
when planting the greening seedlings, surrounding the root systems of the seedlings by using non-woven fabrics, filling root surrounding soil in the non-woven fabrics, filling growth soil between the non-woven fabrics and tree holes, planting the greening seedlings, and watering and managing to obtain the greening seedlings.
2. The method for greening garden engineering according to claim 1, wherein when the garden greening seedlings are planted, a circular tree pit is dug, and the height and depth of the pit are determined according to the size of the transplanted seedlings.
3. The method for greening engineering garden according to claim 1, wherein the raw soil in the tree pit is excavated more than half a year in advance, and the tree pit is fully aired and turned for a plurality of times.
4. The method for greening engineering garden according to claim 1, wherein said nonwoven is degradable nonwoven.
5. The method for greening engineering plants according to claim 1, wherein said seedlings are white wax seedlings.
6. Use of the method of any one of claims 1-5 in landscaping with raw soil.
7. The use of claim 6, wherein the greening project site comprises: roadside, abandoned mines, landfill sites and beside ditches.
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