CN110463564A - A kind of photosynthetic planting soil of raising nursery stock and its application in zelkova schneideriana cultivating seedlings - Google Patents
A kind of photosynthetic planting soil of raising nursery stock and its application in zelkova schneideriana cultivating seedlings Download PDFInfo
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- CN110463564A CN110463564A CN201910898351.1A CN201910898351A CN110463564A CN 110463564 A CN110463564 A CN 110463564A CN 201910898351 A CN201910898351 A CN 201910898351A CN 110463564 A CN110463564 A CN 110463564A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract
The invention discloses a kind of photosynthetic planting soil of raising nursery stock and its applications in zelkova schneideriana cultivating seedlings.Planting soil includes soil matrix and exotrophic mycorrhiza bacterial manure；Wherein, the pH and active principle of soil matrix pass through special regulation, its water and fertilizer condition is controlled in proper level, it is matched with exotrophic mycorrhiza bacterial manure, provides the most suitable growth environment of nursery stock；Exotrophic mycorrhiza bacterial manure includes delicious lactarius, Pisolithus tinctorius and slippery jack, and the volume ratio of delicious lactarius, Pisolithus tinctorius and slippery jack is 1:1~3:1~3.Exotrophic mycorrhiza and root system of plant form mycorhiza structure, it increases host plant root absorption area, improve zelkova schneideriana nursery stock to moisture and Nutrient Absorption, the photosynthesis of plant leaf blade can be greatly improved, promote growth, enhance nursery stock production benefit, is cultivated for rare tree zelkova schneideriana, popularization provides technical support and theoretical foundation.
Description
Technical field
The invention belongs to phytoecology technical fields, and in particular to a kind of photosynthetic planting soil of raising nursery stock and its
Application in zelkova schneideriana cultivating seedlings.
Background technique
Mycorrhizal fungi (Mycorrhiza) be a kind of soil fungi and higher plant root formed there is specific modality structure
With the homobium of function, in plant and mycorrhizal fungi syntaxial system, plant provides carbohydrate for mycorrhizal fungi, and mycorhiza is true
It is the more mineral nutrients of plant absorption and moisture that bacterium, which then passes through its powerful mycelia network,.In recent years, mycorrhizal fungi is applied
To in forest seedling growth and afforestation practice.In trees during introducing a fine variety, nursery stock can be promoted using mycorhiza soil inoculation Introduced Tree Species
Growth and development, improve transplanting survival rate.Exotrophic mycorrhiza (Ecomycorrhizae) is one of seven big fungi types, by infecting
The feeding root of host plant not yet suberification, and colonize to be formed between bacterium set (Mantle) and intrusion cortical cell tissue in root surface
Structures such as Hartig net (Hartig's net) are formed, absorption of the host plant to moisture and mineral nutrient is greatly improved.Nursery stock
Mycorrhizal can enhance nursery stock production benefit.
Zelkova schneideriana (Zelkova schneideriana) belongs to Ulmaceae (Ulmaceae) Zelkova (Zelkova) fallen leaves megaphanerophyte,
It is long with the age of tree, wind resistance, drought resisting, the features such as pest and disease damage is few, material is excellent.But seedling growth is slow, the age of tree is long, the surrival rate of afforestation
Low, available resources are very in short supply.
Mycorrhizal fungi is applied, is improved zelkova schneideriana seedling growth quality (photosynthetic rate), and then improves plant percent.However,
Mycorhiza inoculation effect is mainly limited by Soil Micro-environment.On the one hand, water and fertilizer condition is very good, and mycorrhizal fungi infection rate can decline,
Because many researchs have shown that mycorrhizal fungi infection rate and environment water and fertilizer condition negative correlation；On the other hand, liquid manure item
Part is too poor, and will affect nursery stock normal growth.Therefore a kind of planting soil with reasonable Soil Micro-environment technical indicator is found, is
The effective means for improving zelkova schneideriana transplanting survival rate of nursery stocks, promoting zelkova schneideriana seedling growth rate.
Summary of the invention
For the above-mentioned prior art, the present invention provides a kind of photosynthetic planting soil of raising nursery stock and its in zelkova schneideriana nursery stock
Application in cultivation, to realize the purpose for improving zelkova schneideriana transplanting survival rate of nursery stocks, promoting zelkova schneideriana seedling growth rate.
In order to achieve the above object, the technical scheme adopted by the invention is that: it is photosynthetic to provide a kind of raising nursery stock
Planting soil, the present invention in planting soil include soil matrix and exotrophic mycorrhiza bacterial manure, the body of soil matrix and exotrophic mycorrhiza bacterial manure
Product is than being 10~15:1；The pH of soil matrix is 5.7~6.1, and the content of organic matter is 64g/kg~66g/kg, and full C content is
2.5%~3.0%, full N content be 0.3%~0.4%, active potassic content be 0.1~0.15mg/g, full K content be 18~
20mg/g, available P content are 70~75mg/kg, and full P content is 0.4~0.5mg/g；Exotrophic mycorrhiza bacterial manure includes delicious lactarius, coloured silk
Color beans Lasiosphaera fenzlii and slippery jack, and the volume ratio of delicious lactarius, Pisolithus tinctorius and slippery jack is 1:1~3:1
~3.
Based on the above technical solution, the present invention can also be improved as follows.
Further, soil matrix and the volume ratio of exotrophic mycorrhiza bacterial manure are 11:1.
Further, the pH of soil matrix is 6.0, content of organic matter 64.2g/kg, and full C content is 2.7%, full N content
It is 0.36%, active potassic content is 0.11mg/g, and full K content is 19.2mg/g, and available P content is 73.2mg/kg, and full P content is
0.47mg/g。
Further, the volume ratio of delicious lactarius, Pisolithus tinctorius and slippery jack is 1:1:1 in exotrophic mycorrhiza bacterial manure.
Further, in the present invention ectotrophic mycorrhiza fertilizer the preparation method comprises the following steps: first carry out purifying culture to three kinds of microbial inoculums respectively,
Purifying culture carries out according to a conventional method；Then by microbial inoculum after purification pass through 1~2 generation secondary culture, then be forwarded to vermiculite,
The Medium Culture further expansion that bog moss volume ratio is 11:1 is bred, and expanding numerous required culture medium is MMN fluid nutrient medium, cultivates ring
Border is 25 DEG C of dark surrounds, and cultivation cycle is 1 month；After the numerous culture of expansion in 1 month, by three kinds of microbial inoculums by 1:1~3:1~
3 volume ratio uniformly mixes up to exotrophic mycorrhiza bacterial manure.
Planting soil in the present invention can be used for the cultivation of a variety of trees seedling, can improve the shifting of nursery stock to a certain extent
Survival rate is planted, and promotes the growth rate of nursery stock.Planting soil in the present invention is particularly evident to the cultivate effect of zelkova schneideriana nursery stock, because
This can be used as the dedicated planting soil of zelkova schneideriana cultivating seedlings.When carrying out zelkova schneideriana cultivating seedlings with the planting soil in the present invention, including with
Lower step:
S1: preparing soil matrix, and the 1~2h that sterilizes under conditions of 120~140 DEG C, 0.14MPa；
S2: the exotrophic mycorrhiza bacterial manure of formula ratio is added to soil matrix, mixes thoroughly, obtains planting soil；
S3: taking annual zelkova schneideriana nursery stock, and with sterile water wash root system 3~5 times, and it is 0.2 that root system, which is then immersed concentration,
In~0.3% formalin, soaking time is 8~10min；
S4: the zelkova schneideriana nursery stock after immersion is transplanted by the specification of 1 plant/basin into planting pot, routinely zelkova schneideriana cultivating seedlings
Management means is managed, until zelkova schneideriana nursery stock, which grows to, needs height.
The beneficial effects of the present invention are: content of the present invention by each element in regulation planting soil, by the liquid manure of planting soil
Condition is controlled in medium level, and configures the ectotrophic mycorrhiza under planting soil parameter system with zelkova schneideriana nursery stock with symbiosis feature
Fertilizer can greatly improve the photosynthesis of plant leaf blade, promote growth, enhance nursery stock production benefit, cultivated for rare tree zelkova schneideriana,
Popularization provides technical support and theoretical foundation.
Specific embodiment
Below with reference to embodiment, specific embodiments of the present invention will be described in detail.
Embodiment one
A kind of planting soil, including soil matrix and exotrophic mycorrhiza bacterial manure, the volume ratio of soil matrix and exotrophic mycorrhiza bacterial manure
For 11:1.The pH that soil matrix is adjusted in the present embodiment is 6.0, and content of organic matter 64.2g/kg, full C content is 2.7%, entirely
N content is 0.36%, and active potassic content is 0.11mg/g, and full K content is 19.2mg/g, and available P content is 73.2mg/kg, full P
Content is 0.47mg/g.
The selected exotrophic mycorrhiza bacterial manure of this implementation is delicious lactarius, Pisolithus tinctorius, slippery jack volume ratio are 1:
The mixture of three kinds of microbial inoculums of 1:1.The preparation of exotrophic mycorrhiza bacterial manure is each microbial inoculum first to be purified culture respectively, then purifying is cultivated
Extended end-plate connection afterwards pass through 1~2 generation secondary culture, then be forwarded to vermiculite, bog moss volume ratio be 11:1 Medium Culture into
One step expanding propagation, expanding numerous required culture medium is MMN fluid nutrient medium, and culture environment is 25 DEG C of dark surrounds, cultivation cycle 1
A month；By three kinds of microbial inoculums by volume it is that 1:1:1 is uniformly mixed after 1 month, obtains exotrophic mycorrhiza bacterial manure.
The present embodiment investigates planting soil to transplanting survival rate of nursery stocks and growth rate using annual zelkova schneideriana nursery stock as object
It influences.The transplanting of zelkova schneideriana sapling carries out as follows:
S1: soil matrix, and the 2h that sterilizes under conditions of 120 DEG C, 0.14MPa are prepared；
S2: exotrophic mycorrhiza bacterial manure is added to soil by the dosage of 1.1L soil matrix application 100mL exotrophic mycorrhiza bacterial manure
Matrix is mixed thoroughly, and planting soil is obtained；
S3: taking annual zelkova schneideriana nursery stock, and with sterile water wash root system 4 times, and it is 0.2% that root system, which is then immersed concentration,
In formalin, soaking time 10min；
S4: the zelkova schneideriana nursery stock after immersion is transplanted by the specification of 1 plant/basin into planting pot, routinely zelkova schneideriana cultivating seedlings
Management means is managed, until zelkova schneideriana nursery stock, which grows to, needs height.
Embodiment two
A kind of planting soil, including soil matrix and exotrophic mycorrhiza bacterial manure, the volume ratio of soil matrix and exotrophic mycorrhiza bacterial manure
For 10:1.The pH that soil matrix is adjusted in the present embodiment is 5.7, and content of organic matter 66.1g/kg, full C content is 2.5%, entirely
N content is 0.4%, and active potassic content is 0.14mg/g, and full K content is 18.3mg/g, and available P content is 74.8mg/kg, and full P contains
Amount is 0.41mg/g.
The selected exotrophic mycorrhiza bacterial manure of this implementation is delicious lactarius, Pisolithus tinctorius, slippery jack volume ratio are 1:
The mixture of three kinds of microbial inoculums of 3:1.The preparation of exotrophic mycorrhiza bacterial manure is each microbial inoculum first to be purified culture respectively, then purifying is cultivated
Extended end-plate connection afterwards pass through 1~2 generation secondary culture, then be forwarded to vermiculite, bog moss volume ratio be 11:1 Medium Culture into
One step expanding propagation, expanding numerous required culture medium is MMN fluid nutrient medium, and culture environment is 25 DEG C of dark surrounds, cultivation cycle 1
A month；By three kinds of microbial inoculums by volume it is that 1:3:1 is uniformly mixed after 1 month, obtains exotrophic mycorrhiza bacterial manure.
The present embodiment investigates planting soil to transplanting survival rate of nursery stocks and growth rate using annual zelkova schneideriana nursery stock as object
It influences.The transplanting of zelkova schneideriana sapling carries out as follows:
S1: soil matrix, and the 1.5h that sterilizes under conditions of 130 DEG C, 0.14MPa are prepared；
S2: exotrophic mycorrhiza bacterial manure is added to soil base by the dosage of 1L soil matrix application 100mL exotrophic mycorrhiza bacterial manure
Matter is mixed thoroughly, and planting soil is obtained；
S3: taking zelkova schneideriana nursery stock, and with sterile water wash root system 5 times, and it is molten that root system is then immersed the formaldehyde that concentration is 0.3%
In liquid, soaking time 8min；
S4: the zelkova schneideriana nursery stock after immersion is transplanted by the specification of 1 plant/basin into planting pot, routinely zelkova schneideriana cultivating seedlings
Management means is managed, until zelkova schneideriana nursery stock, which grows to, needs height.
Embodiment three
A kind of planting soil, including soil matrix and exotrophic mycorrhiza bacterial manure, the volume ratio of soil matrix and exotrophic mycorrhiza bacterial manure
For 15:1.The pH that soil matrix is adjusted in the present embodiment is 6.1, and content of organic matter 64.1g/kg, full C content is 3.0%, entirely
N content is 0.3%, and active potassic content is 0.12mg/g, and full K content is 19.9mg/g, and available P content is 70.2mg/kg, and full P contains
Amount is 0.5mg/g.
The selected exotrophic mycorrhiza bacterial manure of this implementation is delicious lactarius, Pisolithus tinctorius, slippery jack volume ratio are 1:
The mixture of three kinds of microbial inoculums of 1:3.The preparation of exotrophic mycorrhiza bacterial manure is each microbial inoculum first to be purified culture respectively, then purifying is cultivated
Extended end-plate connection afterwards pass through 1~2 generation secondary culture, then be forwarded to vermiculite, bog moss volume ratio be 11:1 Medium Culture into
One step expanding propagation, expanding numerous required culture medium is MMN fluid nutrient medium, and culture environment is 25 DEG C of dark surrounds, cultivation cycle 1
A month；By three kinds of microbial inoculums by volume it is that 1:1:3 is uniformly mixed after 1 month, obtains exotrophic mycorrhiza bacterial manure.
The present embodiment investigates planting soil to transplanting survival rate of nursery stocks and growth rate using annual zelkova schneideriana nursery stock as object
It influences.The transplanting of zelkova schneideriana sapling carries out as follows:
S1: soil matrix, and the 1h that sterilizes under conditions of 140 DEG C, 0.14MPa are prepared；
S2: exotrophic mycorrhiza bacterial manure is added to soil by the dosage of 1.5L soil matrix application 100mL exotrophic mycorrhiza bacterial manure
Matrix is mixed thoroughly, and planting soil is obtained；
S3: taking zelkova schneideriana nursery stock, and with sterile water wash root system 5 times, and it is molten that root system is then immersed the formaldehyde that concentration is 0.3%
In liquid, soaking time 8min；
S4: the zelkova schneideriana nursery stock after immersion is transplanted by the specification of 1 plant/basin into planting pot, routinely zelkova schneideriana cultivating seedlings
Management means is managed, until zelkova schneideriana nursery stock, which grows to, needs height.
Comparative example one
Zelkova schneideriana seedling is cultivated with commonly transplanting soil, and adds exotrophic mycorrhiza bacterial manure not in cultivating soil.Zelkova schneideriana
Sapling transplants process with embodiment one.
Comparative example two
Zelkova schneideriana seedling is cultivated with commonly transplanting soil, and adds ectotrophic mycorrhiza in cultivating soil, exotrophic mycorrhiza
Bacterial manure is the mixture of delicious lactarius, Pisolithus tinctorius, three kinds of microbial inoculums that slippery jack volume ratio is 1:1:1.Zelkova schneideriana sapling
Transplanting process is the same as embodiment one.
Comparative example three
The cultivation of zelkova schneideriana seedling is carried out with the soil matrix deployed in the present invention, the pH of soil matrix is 6.0, and the content of organic matter is
64.2g/kg, full C content are 2.7%, and full N content is 0.36%, and active potassic content is 0.11mg/g, and full K content is 19.2mg/
G, available P content are 73.2mg/kg, and full P content is 0.47mg/g；Exotrophic mycorrhiza bacterial manure is added not in cultivating soil.Zelkova schneideriana
Sapling transplants process with embodiment one.
Interpretation of result
Investigate photosynthetic rate (μm ol CO of zelkova schneideriana nursery stock during the growth process in each embodiment and comparative example2m-2), stomata
Degree of leading (mol H2O m-2s-1), intercellular CO2Concentration (μm ol CO2mol-1), transpiration rate (mmol H2O m s-1), water utilization
Efficiency (μm ol CO2/mmol H2O) situations such as, it is as a result listed in table 1, every group of investigation zelkova schneideriana nursery stock quantity is 6 plants.
The control of 1 Photosynthetic Index of table
From table 1 it follows that being cultivated using the planting soil in embodiment to zelkova schneideriana nursery stock, photosynthetic rate and moisture
Utilization rate is cultivated to have and significantly be promoted relative to the cultivation soil used in comparative example, is shown using the plantation in the present invention
Soil carries out stock growing, and the cultivation of especially progress zelkova schneideriana nursery stock can increase substantially the photosynthesis of plant leaf blade, promote
Plant survives in a short time, promotes nursery stock fast-growth.
Plant height (cm), ground diameter (mm) and the branch amount (a) in each experimental group after zelkova schneideriana seedling growth 1 year have been investigated simultaneously
Etc. indexs (being selected before plant with a collection of zelkova schneideriana sapling, height is essentially identical), be as a result listed in table 2.
The control of 2 growth indexes of table
From Table 2, it can be seen that carrying out zelkova schneideriana stock growing using the planting soil in the present invention, zelkova schneideriana seedling can be dramatically increased
The plant height of wood, and ground diameter and branch amount have corresponding promotion, show that the planting soil in the present invention can dramatically increase zelkova schneideriana seedling
The growth rate of wood shortens training seedling period, remarkable in economical benefits.
Although be described in detail to a specific embodiment of the invention in conjunction with the embodiments, should not be construed as to this
The restriction of the protection scope of patent.In range described by claims, those skilled in the art are without creative work
The various modifications and deformation that can make still belong to the protection scope of this patent.
Claims (6)
1. a kind of photosynthetic planting soil of raising nursery stock, it is characterised in that: described including soil matrix and exotrophic mycorrhiza bacterial manure
Soil matrix and the volume ratio of exotrophic mycorrhiza bacterial manure are 10~15:1；The pH of the soil matrix is 5.7~6.1, and organic matter contains
Amount is 64g/kg~66g/kg, and full C content is 2.5%~3.0%, and full N content is 0.3%~0.4%, and active potassic content is 0.1
~0.15mg/g, full K content are 18~20mg/g, and available P content is 70~75mg/kg, and full P content is 0.4~0.5mg/g；
The exotrophic mycorrhiza bacterial manure includes delicious lactarius, Pisolithus tinctorius and slippery jack, and delicious lactarius, Pisolithus tinctorius and brown
The volume ratio of ring Suillus bovines is 1:1~3:1~3.
2. the photosynthetic planting soil of raising nursery stock according to claim 1, it is characterised in that: the soil matrix and outer
The volume ratio of raw mycorhiza bacterial manure is 11:1.
3. the photosynthetic planting soil of raising nursery stock according to claim 1, it is characterised in that: the pH of the soil matrix
It is 6.0, content of organic matter 64.2g/kg, full C content is 2.7%, and full N content is 0.36%, and active potassic content is 0.11mg/
G, full K content are 19.2mg/g, and available P content is 73.2mg/kg, and full P content is 0.47mg/g.
4. the photosynthetic planting soil of raising nursery stock according to claim 1, it is characterised in that: the exotrophic mycorrhiza bacterial manure
The volume ratio of middle delicious lactarius, Pisolithus tinctorius and slippery jack is 1:1:1.
5. such as application of the described in any item planting soils of Claims 1 to 4 in zelkova schneideriana cultivating seedlings.
6. application according to claim 5, which comprises the following steps:
S1: preparing soil matrix, and the 1~2h that sterilizes under conditions of 120~140 DEG C, 0.14MPa；
S2: the exotrophic mycorrhiza bacterial manure of formula ratio is added to soil matrix, mixes thoroughly, obtains planting soil；
S3: taking annual zelkova schneideriana nursery stock, and with sterile water wash root system 3~5 times, then by root system immerse concentration be 0.2~
In 0.3% formalin, soaking time is 8~10min；
S4: the zelkova schneideriana nursery stock after immersion is transplanted into the routinely zelkova schneideriana cultivating seedlings management into planting pot by the specification of 1 plant/basin
Means are managed, until zelkova schneideriana nursery stock, which grows to, needs height.
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