CN102965319B - Microbial agent and bio-organic fertilizer special for winter jujube and preparation methods thereof - Google Patents
Microbial agent and bio-organic fertilizer special for winter jujube and preparation methods thereof Download PDFInfo
- Publication number
- CN102965319B CN102965319B CN2012105015324A CN201210501532A CN102965319B CN 102965319 B CN102965319 B CN 102965319B CN 2012105015324 A CN2012105015324 A CN 2012105015324A CN 201210501532 A CN201210501532 A CN 201210501532A CN 102965319 B CN102965319 B CN 102965319B
- Authority
- CN
- China
- Prior art keywords
- winter jujube
- promoting
- subtilis
- liquid
- organic fertilizer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 230000000813 microbial effect Effects 0.000 title claims abstract description 30
- 239000003895 organic fertilizer Substances 0.000 title claims abstract description 28
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 21
- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- 240000008866 Ziziphus nummularia Species 0.000 title 1
- 241001247821 Ziziphus Species 0.000 claims abstract description 109
- 239000007788 liquid Substances 0.000 claims abstract description 63
- 239000003337 fertilizer Substances 0.000 claims abstract description 38
- 230000000694 effects Effects 0.000 claims abstract description 28
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 24
- 238000000855 fermentation Methods 0.000 claims abstract description 8
- 230000004151 fermentation Effects 0.000 claims abstract description 8
- 241000894006 Bacteria Species 0.000 claims description 45
- 244000005700 microbiome Species 0.000 claims description 23
- 241001468157 Lactobacillus johnsonii Species 0.000 claims description 20
- 239000012752 auxiliary agent Substances 0.000 claims description 19
- 239000002131 composite material Substances 0.000 claims description 18
- 239000002068 microbial inoculum Substances 0.000 claims description 17
- 238000012360 testing method Methods 0.000 claims description 17
- 239000002609 medium Substances 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 10
- 210000003608 fece Anatomy 0.000 claims description 8
- 239000010871 livestock manure Substances 0.000 claims description 8
- 238000011218 seed culture Methods 0.000 claims description 8
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 claims description 7
- 235000015097 nutrients Nutrition 0.000 claims description 7
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 5
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 5
- 239000006229 carbon black Substances 0.000 claims description 5
- 238000009736 wetting Methods 0.000 claims description 5
- 229920001285 xanthan gum Polymers 0.000 claims description 5
- 239000000230 xanthan gum Substances 0.000 claims description 5
- 229940082509 xanthan gum Drugs 0.000 claims description 5
- 235000010493 xanthan gum Nutrition 0.000 claims description 5
- 241000287828 Gallus gallus Species 0.000 claims description 4
- 238000009264 composting Methods 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 claims description 4
- 239000011368 organic material Substances 0.000 claims description 4
- 238000010564 aerobic fermentation Methods 0.000 claims description 3
- 239000001888 Peptone Substances 0.000 claims description 2
- 108010080698 Peptones Proteins 0.000 claims description 2
- 229920002472 Starch Polymers 0.000 claims description 2
- 229940041514 candida albicans extract Drugs 0.000 claims description 2
- 239000002054 inoculum Substances 0.000 claims description 2
- 235000019319 peptone Nutrition 0.000 claims description 2
- 239000008107 starch Substances 0.000 claims description 2
- 235000019698 starch Nutrition 0.000 claims description 2
- 239000012138 yeast extract Substances 0.000 claims description 2
- 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 claims 1
- 230000001580 bacterial effect Effects 0.000 abstract description 13
- 239000000203 mixture Substances 0.000 abstract description 9
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 abstract description 4
- 238000002156 mixing Methods 0.000 abstract description 4
- 230000001737 promoting effect Effects 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 3
- 244000199866 Lactobacillus casei Species 0.000 abstract description 2
- 235000013958 Lactobacillus casei Nutrition 0.000 abstract description 2
- 229940017800 lactobacillus casei Drugs 0.000 abstract description 2
- 244000063299 Bacillus subtilis Species 0.000 abstract 2
- 235000014469 Bacillus subtilis Nutrition 0.000 abstract 2
- 230000000087 stabilizing effect Effects 0.000 abstract 2
- 239000002689 soil Substances 0.000 description 7
- 238000011160 research Methods 0.000 description 5
- 238000010790 dilution Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- 238000011081 inoculation Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- 235000015816 nutrient absorption Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011321 prophylaxis Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000003900 soil pollution Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000002352 surface water Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
Images
Landscapes
- Fertilizers (AREA)
Abstract
The invention discloses microbial composition bacterial liquid comprising the components of winter jujube rhizosphere growth promoting bacillus subtilis DZ1, saccharomyces cerevisiae and lactobacillus casei. The invention also discloses a microbial agent special for winter jujube and a preparation method thereof. The microbial agent is formed in a way of mixing the microbial composition bacterial liquid and an effect stabilizing assistant according to the weight ratio of 100: (4-7). The invention additionally discloses a bio-organic fertilizer special for the winter jujube which is formed in way of mixing the microbial composition bacterial liquid and an organic fertilizer, wherein the ratio of the organic fertilizer to the microbial composition bacterial liquid is 10: (1-2)(W/V). Proved by experiments, compared with a winter jujube seedling which is singly inoculated by using three kinds of bacterial strain fermentation liquor or promotion stabilizing assistants, a winter jujube seedling which is inoculated by using the microbial agent disclosed by the invention has the advantages that the root weight, the ground diameter and the seedling height of the winter jujube are remarkably increased; and compared with a winter jujube fruiting tree which is applied by a bio-fertilizer formed in a way of inoculating the winter jujube rhizosphere growth promoting bacillus subtilis DZ1 into the organic fertilizer or the organic fertilizer, a winter jujube fruiting tree which is applied by the bio-organic fertilizer disclosed by the invention has the advantages that the yield is improved by 10-20% and the quality is obviously improved.
Description
Technical field
The present invention relates to a kind of winter jujube special microorganism microbial inoculum, biological organic fertilizer and preparation method thereof.
Background technology
The production management process of winter jujube is very complicated, and fertilising is one of them very important link.Fertilization Effect is except the impact that is subject to soil, the nutritive property of plant own and rate of fertilizer application etc., also relevant with fertilizer variety etc.The fertilizer that present stage uses in the winter jujube, the organic fertilizer that compound manure, the farm manure that is mainly the chemical fertilizer combination is the master etc., compound manure and organic fertilizer compounding application have improved yield of winter jujubes and quality.Because winter jujube root system is undeveloped, poor growth, capillary radical amount is few, and poor nutrient absorption capability is used composite fertilizer in a large number, and the fertilizer nutrient leaching loss is serious, has caused the eutrophication of surface water and the pollution of underground water; Though the farm manure of take is main organic fertilizer and can improves soil physico-chemical property, the germ that organic character wherein is very different, contain and sick worm's ovum etc. easily cause soil pollution.Microbial fertilizer can not only be improved soil, improves the ecotope of soil fauna, can also effectively improve plant absorbing soil nutrient.Therefore, seek the new-type fertilizer that is applicable to the winter jujube, especially microbial fertilizer, receive much attention with the research that reduces fertilizer amount, lifting fertilizer effect.
The domestic report of the research about winter jujube microbial fertilizer is less.CN102643144A discloses a kind of winter jujube special active biological medicine fertilizer, main as winter jujube foliage spray, there is the jujube growth of the winter of promotion, have to increase and spend, improve setting percentage, disinsection prophylaxis, the effect that the degraded agriculture is residual, joint props up volume increase, improves winter jujube quality, have diseases prevention and getting fat double effects concurrently.Jujube special active biological medicine fertilizer of disclosed winter of this patent is foliar nutrient, for the improvement of soil and the not effect of improvement of rhizosphere environment.
Research shows: the interaction of functional microorganism and crop is often also unstable, and this unstable comes from the varying environment condition microorganism growth is impacted, thereby restricts its effect.This just requires the functional microorganism in microbial fertilizer should have more specific aim.The at present domestic research report of producing for the winter jujube about microbial fertilizer is less, does not see the research report about winter jujube special microorganism composite bacterial agent.
For the winter jujube, development special microorganism composite bacterial agent and bio-organic fertilizer special, stablize the soil microenvironment that improves continued, and with the output that improves the winter jujube, the quality of improving the winter jujube, is the active demand that current winter jujube is produced.
The inventor has filtered out a strain winter jujube rhizosphere growth-promoting subtilis DZ1 from winter jujube Rhizosphere sampling, and deposit number is CGMCC No.5318, can obviously promote that the winter jujube grows, and improves winter jujube rhizosphere environment.For this bacterial strain and application thereof, the inventor has applied for national inventing patent " a strain winter jujube rhizosphere growth-promoting subtilis and application " thereof, number of patent application 201110376966.1.Patent discloses the application of winter jujube rhizosphere growth-promoting subtilis DZ1: cultivate this bacterial strain, obtain bacteria suspension, bacteria suspension is as liquid fertilizer; Bacterial suspension inoculation, in fertilizer, is made to the Winter jujube biological fertilizer.These application are only preliminary, are in particular in: the microbial fertilizer that (1) above-mentioned application is made, only containing winter jujube rhizosphere growth-promoting subtilis DZ1, needs to add multiple specified microorganisms population, to realize the cluster growgh promoting effects of microorganism; (2) fermentation condition of winter jujube rhizosphere growth-promoting subtilis DZ1 needs further to optimize, to realize suitability for industrialized production; (3) bacteria suspension is directly as liquid fertilizer, and its quality guaranteed period is shorter, need add suitable stablizer and protective material, with the survival of protection viable bacteria, extends the shelf life.
Summary of the invention
The object of the present invention is to provide a kind of microbial composite bacterium liquid, antagonism not between each bacterial strain, collaborative mutually, in order to prepare winter jujube special microorganism microbial inoculum, biological organic fertilizer.
Another object of the present invention is to provide a kind of winter jujube special microorganism microbial inoculum that contains mentioned microorganism composition bacterium liquid and preparation method thereof.
Another object of the present invention is to provide a kind of winter jujube bio-organic fertilizer special material that contains mentioned microorganism composition bacterium liquid and preparation method thereof.
For achieving the above object, the invention provides following technical scheme:
A kind of microbial composite bacterium liquid, comprise winter jujube rhizosphere growth-promoting subtilis DZ1, S. cervisiae, lactobacillus johnsonii.Test shows: between three bacterial strains without antagonistic action.
The ratio of the cfu of described winter jujube rhizosphere growth-promoting subtilis DZ1, described S. cervisiae, described lactobacillus johnsonii is: 10:1～2:2～3, microbial composite bacterium liquid is containing viable count>3 * 10
8cfu/ml.
A kind of microbiobacterial agent that is exclusively used in the winter jujube that the present invention provides in addition, to be mixed by weight 100:4～7 by mentioned microorganism composition bacterium liquid and steady effect auxiliary agent, the ratio of the cfu of the liquid medium of described winter jujube rhizosphere growth-promoting subtilis DZ1, S. cervisiae, lactobacillus johnsonii is: 10:1～2:2～3, microbial composite bacterium liquid is containing viable count>3 * 10
8cfu/ml.
Microbial composite bacterium liquid contains total living bacteria count with the mixed microbiobacterial agent of steady effect auxiliary agent>2.5 * 10
8cfu/ml.
Described steady effect auxiliary agent is comprised of the component of following weight proportion: 3 ~ 5 parts, calcium carbonate, 0.2 ~ 0.5 part of xanthan gum, 0.2 ~ 0.3 part of wetting ability White Carbon black, 0.3 ~ 0.6 part, sodium-chlor, Xylo-Mucine 0.3-0.6 part.
This is exclusively used in the preparation method of the microbiobacterial agent of winter jujube, and step is as follows:
(1) get winter jujube rhizosphere growth-promoting subtilis DZ1, S. cervisiae, lactobacillus johnsonii and cultivated respectively to obtain fermented liquid;
(2) by fermented liquid, by the ratio of cfu, be: 10:1～2:2～3 are mixed to get mixed bacteria liquid, and mixed bacteria liquid is mixed with steady effect auxiliary agent, obtain.
The cultural method of described winter jujube rhizosphere growth-promoting subtilis DZ1 is (below be weight percentage):
(1) test tube seed culture
The seed culture based formulas is, in the gross weight of seed culture medium: extractum carnis 0.3%, peptone 1%, sodium-chlor 0.5%, pH7.0-7.2, surplus is water;
Winter jujube rhizosphere growth-promoting subtilis DZ1 is inoculated on seed culture medium, and 30～35 ℃ of constant temperature culture 36 hours, obtain the test tube kind.
(2) liquid fermentation and culture
The liquid fermentation medium formula is, in the total amount of liquid fermentation medium: Zulkovsky starch 1%, yeast extract paste 1%, K
2hPO
40.15%, MgSO
47H
2o0.02%, CaCl
20.01%, surplus is water, pH6.5-7.5.
The test tube seed is inoculated in 500ml triangular flask liquid nutrient medium to inoculum size 10%(volume ratio), every bottled 200ml, in 30～35 ℃ of shaking tables, cultivate, rotating speed 180r/min, cultivate 12 hours, and in fermented liquid, winter jujube rhizosphere growth-promoting subtilis DZ1 bacteria containing amount can reach 40 * 10
8cfu/ml.
The present invention also provides a kind of biological organic fertilizer that is exclusively used in the winter jujube, that liquid medium and fertilizer by winter jujube rhizosphere growth-promoting subtilis DZ1, S. cervisiae, lactobacillus johnsonii mixes, described fertilizer: microbial composite bacterium liquid=10:1 ~ 2(W/V).
Described biological organic fertilizer is containing total living bacteria count>=0.25 * 10
8cfu/g.
The making of described fertilizer: chicken manure and bacterium chaff are mixed by weight 9:1, inoculate the organic material composting microbial inoculum, carry out aerobic fermentation, become thoroughly decomposed after air-dry and pulverized 1～2mm sieve, water content≤20%, organic content>=30%, total nutrient N+P
2o
5+ K
2o>=5%.Chicken manure, bacterium chaff and organic material composting microbial inoculum all can from market purchasing to.
S. cervisiae (saccharomyces cerevisiae), lactobacillus johnsonii (Lactobacillus casei) that the present invention uses derive from China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), the S. cervisiae bacterium numbering is 1.0580, and the lactobacillus johnsonii bacterium numbering is 2.0666.Can buy by commercial sources, the explanation that their cultivation and growth conditions provide by culture presevation unit is carried out.
The present invention compared with prior art, has following effect and advantage:
(1) to take winter jujube rhizosphere growth-promoting subtilis DZ1 be main ingredient in the present invention, and optimum combination other function yeast, realized the cluster growgh promoting effects of many bacterial strains to the winter jujube, make the microbial fertilizer product there is stronger specific aim and validity for the winter jujube.
(2) the invention provides the optimization cultural method of winter jujube rhizosphere growth-promoting subtilis DZ1, in the situation that production cost does not increase, in fermented liquid, bacteria containing amount reaches 40 * 10
8cfu/ml, productivity effect is significantly improved.
(3) in microbiobacterial agent of the present invention, the interpolation of stablizer has guaranteed activity and the stability of function yeast.
(4) evidence, winter jujube seedling inoculation microbiobacterial agent of the present invention is than the winter jujube rhizosphere growth-promoting subtilis DZ1 fermented liquid of single inoculation, yeast saccharomyces cerevisiae fermented liquid, lactobacillus johnsonii fermented liquid or surely during short auxiliary agent, and winter jujube root is heavy, leading thread and height of seedling all significantly increase; Winter jujube bearing-age tree is used biological organic fertilizer of the present invention and is inoculated into bio-feritlizer or the fertilizer made in fertilizer and compares with using winter jujube rhizosphere growth-promoting subtilis DZ1, and output improves 10～20%, and quality is obviously improved.
The accompanying drawing explanation
Fig. 1 is process flow sheet of the present invention;
Fig. 2 is the living bacteria count test-results figure of winter jujube special microorganism microbial inoculum of the present invention.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated.
Embodiment 1 is exclusively used in the microbiobacterial agent of winter jujube, the preparation of biological organic fertilizer
Cultural method by aforementioned winter jujube rhizosphere growth-promoting subtilis DZ1 prepares winter jujube rhizosphere growth-promoting subtilis DZ1 fermented liquid.
The cultural method provided by culture presevation unit prepares the fermented liquid of yeast saccharomyces cerevisiae fermented liquid, lactobacillus johnsonii.
Quantity ratio by the gained fermented liquid by contained viable count mixes: ratio=10:1.5:2.5 of the cfu of winter jujube rhizosphere growth-promoting subtilis DZ1, described S. cervisiae, described lactobacillus johnsonii.Microbial composite bacterium liquid is containing total viable count 3.36 * 10
8cfu/ml.
Steady effect auxiliary agent forms according to following weight proportion component: 3.5 parts, calcium carbonate, 0.35 part of xanthan gum, 0.2 part of wetting ability White Carbon black, 0.5 part, sodium-chlor, 0.5 part of Xylo-Mucine.Microbial composite bacterium liquid and steady effect auxiliary agent are made winter jujube special microorganism microbial inoculum after mixing and mix by weight 100:5.05, and total viable count is 3.10 * 10
8cfu/ml.
Chicken manure and bacterium chaff are mixed by weight 9:1, inoculate the organic material composting microbial inoculum, carry out aerobic fermentation, become thoroughly decomposed after air-dry and pulverized the 2mm sieve, make water content≤20%, organic content>=30%, total nutrient N+P
2o
5+ K
2the fertilizer of O>=5%.
According to fertilizer: microbial composite bacterium liquid=10:1 ratio is fully adsorbed and is mixed, and makes winter jujube bio-organic fertilizer special.In fertilizer, viable count is 0.28 * 10
8cfu/g.
Embodiment 2 is exclusively used in the microbiobacterial agent of winter jujube
Cultural method by aforementioned winter jujube rhizosphere growth-promoting subtilis DZ1 prepares winter jujube rhizosphere growth-promoting subtilis DZ1 fermented liquid.
The cultural method provided by culture presevation unit prepares the fermented liquid of yeast saccharomyces cerevisiae fermented liquid, lactobacillus johnsonii.
Quantity ratio by the gained fermented liquid by contained viable count mixes: ratio=10:1.3:2.3 of the cfu of winter jujube rhizosphere growth-promoting subtilis DZ1, described S. cervisiae, described lactobacillus johnsonii.Microbial composite bacterium liquid is containing total viable count 3.64 * 10
8cfu/ml.
Steady effect auxiliary agent forms according to following weight proportion component: 4.0 parts, calcium carbonate, 0.30 part of xanthan gum, 0.2 part of wetting ability White Carbon black, 0.6 part, sodium-chlor, 0.4 part of Xylo-Mucine.Microbial composite bacterium liquid and steady effect auxiliary agent are made winter jujube special microorganism microbial inoculum after mixing and mix by weight 100:5.5, and total viable count is 3.41 * 10
8cfu/ml.
Embodiment 3 winter jujube special microorganism microbial inoculum application test
Test arrangement is in test nursery, forest-science academy, Shandong Province.Choose the consistent annual winter jujube seedling of growth, field planting is at high 20cm, in the plastic tub of diameter 30cm, gets for the winter jujube special microorganism microbial inoculum that embodiment 1 makes 10 times of tap water dilutions, gets microbial inoculum (the total living bacteria count 40 * 10 after the 100ml dilution
8cfu) evenly water around winter jujube shoot root section, grow 6 months the time and measure overground part dry weight, underground part dry weight, gross dry weight (experimental group), and with pouring clear water 100ml(control group 1), winter jujube rhizosphere growth-promoting subtilis DZ1 fermented liquid (the total living bacteria count 40 * 10 after the pouring dilution only
8cfu) the 100ml(control group 2), only water yeast saccharomyces cerevisiae fermented liquid (the total living bacteria count 40 * 10 after diluting
8cfu) 100ml(control group, 3), fermented liquid (total living bacteria count 40 * 10 of the lactobacillus johnsonii after the pouring dilution only
8cfu) the 100ml(control group 4) contrasted.The results are shown in Table 1.
As can be seen from Table 1: the biomass indexes such as the overground part dry weight of experimental group processing winter jujube nursery stock, underground part dry weight, gross dry weight are all higher than control group, and difference all reaches conspicuous level.Show: winter jujube special microorganism microbial inoculum has obvious promoter action to winter jujube growth.
The growth-promoting functions of table 1 microbiobacterial agent to winter jujube nursery stock
Embodiment 4 winter jujube bio-organic fertilizer special field test
Winter jujube biological fertilizer field test is arranged in Accessories during Binzhou forestry bureau winter jujube Demonstration Garden, and the age of tree is 8 years.Use the winter jujube bio-organic fertilizer special (experimental group) that embodiment 1 makes, with the fertilizer made with embodiment 1 (control group 1), the biological organic fertilizer (0.28 * 10 made from the fertilizer absorption winter jujube rhizosphere growth-promoting subtilis DZ1 fermented liquid made by embodiment 1
8cfu/g) (control group 2) contrasted.In application in two dressings in 2011: it is fertile or contrast fertile 5kg that every tree is used test by the end of June, and it is fertile or contrast fertile 10kg that at the beginning of 9 months, every tree is used test, adopts the south and north flanks ditch spread, and Physicochemical fertilizer is normally used everywhere.Measure the yield and quality of winter jujube on October 9th, 2012, the results are shown in Table 2.
As can be seen from the test results, use the fruit quality of winter jujube bio-organic fertilizer special and output all higher than control group, difference all reaches conspicuous level.
The impact of table 2 biological organic fertilizer on the winter-jujube fruit yield and quality
The living bacteria count test of embodiment 5 winter jujube special microorganism microbial inoculum
Adopt the microbiobacterial agent that is exclusively used in the winter jujube in embodiment 2, with only add steady effect auxiliary agent in calcium carbonate (control group 1), only add xanthan gum (control group 2) in steady effect auxiliary agent, only add wetting ability White Carbon black (control group 3) in steady effect auxiliary agent, only add sodium-chlor (control group 4) in steady effect auxiliary agent, only add the Xylo-Mucine (control group 5) in steady effect auxiliary agent and do not add any steady effect auxiliary agent (control group 6) and contrasted, measure the viable count in microbial inoculum after natural storage 90d, result as shown in Figure 2.Can find out, the living bacteria count in winter jujube microbiobacterial agent significantly is greater than the test of adding the steady effect of part auxiliary agent and processes.
Claims (7)
1. a microbial composite bacterium liquid that is exclusively used in the winter jujube, it is characterized in that, comprise winter jujube rhizosphere growth-promoting subtilis DZ1, S. cervisiae, lactobacillus johnsonii, wherein, the ratio of the cfu of described winter jujube rhizosphere growth-promoting subtilis DZ1, described S. cervisiae, described lactobacillus johnsonii is: 10:1～2:2～3, microbial composite bacterium liquid is containing viable count>3 * 10
8cfu/ml; Described winter jujube rhizosphere growth-promoting subtilis DZ1 Classification And Nomenclature is bacillus sp., on October 11st, 2011, is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and its deposit number is CGMCC No.5318.
2. a microbiobacterial agent that is exclusively used in the winter jujube, it is characterized in that, to be mixed by weight 100:4～7 by the liquid medium of winter jujube rhizosphere growth-promoting subtilis DZ1, S. cervisiae, lactobacillus johnsonii and steady effect auxiliary agent, the ratio of the cfu of the liquid medium of described winter jujube rhizosphere growth-promoting subtilis DZ1, S. cervisiae, lactobacillus johnsonii is: 10:1～2:2～3, microbial composite bacterium liquid is containing viable count>3 * 10
8cfu/ml;
Described steady effect auxiliary agent is comprised of the component of following weight proportion: 3～5 parts, calcium carbonate, 0.2～0.5 part of xanthan gum, 0.2～0.3 part of wetting ability White Carbon black, 0.3～0.6 part, sodium-chlor, Xylo-Mucine 0.3-0.6 part.
3. the microbiobacterial agent that is exclusively used in the winter jujube according to claim 2, is characterized in that, described microbiobacterial agent is containing total living bacteria count>=2.5 * 10
8cfu/ml.
4. the preparation method who is exclusively used in the microbiobacterial agent of winter jujube claimed in claim 2, is characterized in that, step is as follows:
(1) get winter jujube rhizosphere growth-promoting subtilis DZ1, S. cervisiae, lactobacillus johnsonii and cultivated respectively to obtain fermented liquid;
(2) by fermented liquid, by the ratio of the cfu of winter jujube rhizosphere growth-promoting subtilis DZ1, S. cervisiae, lactobacillus johnsonii, be: 10:1～2:2～3 are mixed to get mixed bacteria liquid, and mixed bacteria liquid is mixed with steady effect auxiliary agent, obtain.
5. the preparation method who is exclusively used in the microbiobacterial agent of winter jujube claimed in claim 4, is characterized in that, the cultural method of described winter jujube rhizosphere growth-promoting subtilis DZ1 is below to be weight percentage:
(1) test tube seed culture
The seed culture based formulas is, in the gross weight of seed culture medium: extractum carnis 0.3%, peptone 1%, sodium-chlor 0.5%, pH7.0-7.2, surplus is water;
Winter jujube rhizosphere growth-promoting subtilis DZ1 is inoculated on seed culture medium, and 30～35 ℃ of constant temperature culture 36 hours, obtain the test tube kind;
(2) liquid fermentation and culture
The liquid fermentation medium formula is, in the total amount of liquid fermentation medium: Zulkovsky starch 1%, yeast extract paste 1%, K
2hPO
40.15%, MgSO
47H
2o0.02%, CaCl
20.01%, surplus is water, pH6.5-7.5;
The test tube seed is inoculated in 500ml triangular flask liquid nutrient medium, and inoculum size is 10% by volume, every bottled 200ml, in 30～35 ℃ of shaking tables, cultivate, rotating speed 180r/min, cultivate 12 hours, and in fermented liquid, winter jujube rhizosphere growth-promoting subtilis DZ1 bacteria containing amount can reach 40 * 10
8cfu/ml.
6. a biological organic fertilizer that is exclusively used in the winter jujube, it is characterized in that, that liquid medium and fertilizer by winter jujube rhizosphere growth-promoting subtilis DZ1, S. cervisiae, lactobacillus johnsonii mixes, described fertilizer: microbial composite bacterium liquid=10:1～2(W/V); Described biological organic fertilizer is containing total living bacteria count>=0.25 * 10
8cfu/g.
7. the biological organic fertilizer that is exclusively used in the winter jujube according to claim 6, it is characterized in that, the making of described fertilizer: chicken manure and bacterium chaff are mixed by weight 9:1, inoculate the organic material composting microbial inoculum, carry out aerobic fermentation, become thoroughly decomposed after air-dry and pulverized 1～2mm sieve, water content≤20%, organic content>=30%, total nutrient N+P
2o
5+ K
2o>=5%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012105015324A CN102965319B (en) | 2012-11-30 | 2012-11-30 | Microbial agent and bio-organic fertilizer special for winter jujube and preparation methods thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012105015324A CN102965319B (en) | 2012-11-30 | 2012-11-30 | Microbial agent and bio-organic fertilizer special for winter jujube and preparation methods thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102965319A CN102965319A (en) | 2013-03-13 |
| CN102965319B true CN102965319B (en) | 2013-12-11 |
Family
ID=47795755
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012105015324A Expired - Fee Related CN102965319B (en) | 2012-11-30 | 2012-11-30 | Microbial agent and bio-organic fertilizer special for winter jujube and preparation methods thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102965319B (en) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104649847B (en) * | 2015-02-27 | 2017-05-31 | 中国热带农业科学院椰子研究所 | A kind of betel nut flower-promoting and fruit protecting special fertilizer and preparation method thereof |
| CN104788177B (en) * | 2015-03-31 | 2016-03-02 | 山东省林业科学研究院 | Special water conservation biological organic fertilizer of blueberry and preparation method thereof |
| CN104726103B (en) * | 2015-03-31 | 2016-03-09 | 山东省林业科学研究院 | A kind of drought mountain microorganism combining bacteria liquid and gel preparation thereof |
| CN106631546A (en) * | 2016-09-08 | 2017-05-10 | 西北农林科技大学 | Bio-organic fertilizer and preparation method thereof |
| CN106912310A (en) * | 2017-03-15 | 2017-07-04 | 山东省林业科学研究院 | A kind of utilization root system pruning and rhizosphere nutrition regulation and control mitigate the method that winter jujube opens first intensity |
| CN108101612B (en) * | 2018-02-05 | 2021-01-01 | 山东省林业科学研究院 | Selenium-rich bio-organic fertilizer special for winter jujubes and preparation and application methods thereof |
| CN113185346A (en) * | 2020-01-10 | 2021-07-30 | 广西泥微生物科技有限公司 | Organic fertilizer special for jujube and preparation method thereof |
| CN112279693A (en) * | 2020-09-23 | 2021-01-29 | 黑龙江省农业科学院畜牧研究所 | Biological organic fertilizer and preparation method thereof |
| CN113667617A (en) * | 2021-08-06 | 2021-11-19 | 甘肃农业大学 | Special microbial agent for natural high-cold grassland and preparation method and application thereof |
| CN115777489B (en) * | 2022-11-23 | 2023-06-02 | 山东省林业科学研究院 | Garden engineering biological greening method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102379374A (en) * | 2011-11-02 | 2012-03-21 | 王和松 | Microbial high-activity preparation, high-activity microbial protein forage and preparation method thereof |
| CN102409014A (en) * | 2011-11-23 | 2012-04-11 | 山东省林业科学研究院 | Ziziphus jujube mill photosphere rhizosphere bacillus sp. capable of promoting growth |
| CN102432350A (en) * | 2011-09-16 | 2012-05-02 | 北京平安福生物技术研究所有限公司 | Biological organic fertilizer used for fruit trees and preparation method thereof |
| CN102432399A (en) * | 2011-09-16 | 2012-05-02 | 北京平安福生物技术研究所有限公司 | Biological organic fertilizer for improving stress resistance and output of paddy rice and preparation method thereof |
-
2012
- 2012-11-30 CN CN2012105015324A patent/CN102965319B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102432350A (en) * | 2011-09-16 | 2012-05-02 | 北京平安福生物技术研究所有限公司 | Biological organic fertilizer used for fruit trees and preparation method thereof |
| CN102432399A (en) * | 2011-09-16 | 2012-05-02 | 北京平安福生物技术研究所有限公司 | Biological organic fertilizer for improving stress resistance and output of paddy rice and preparation method thereof |
| CN102379374A (en) * | 2011-11-02 | 2012-03-21 | 王和松 | Microbial high-activity preparation, high-activity microbial protein forage and preparation method thereof |
| CN102409014A (en) * | 2011-11-23 | 2012-04-11 | 山东省林业科学研究院 | Ziziphus jujube mill photosphere rhizosphere bacillus sp. capable of promoting growth |
Non-Patent Citations (2)
| Title |
|---|
| 刘方春等人.生物肥对冬枣根际土壤微环境特征的影响.《北京林业大学学报》.2012,第34卷(第5期),100-104. |
| 生物肥对冬枣根际土壤微环境特征的影响;刘方春等人;《北京林业大学学报》;20120930;第34卷(第5期);100-104 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102965319A (en) | 2013-03-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102965319B (en) | Microbial agent and bio-organic fertilizer special for winter jujube and preparation methods thereof | |
| CN103964946B (en) | A kind of paddy rice Special compound microbial fertilizer and preparation method thereof | |
| CN103848698B (en) | A kind of biological organic fertilizer utilizing natural pond slag aerobic fermentation to prepare and preparation method thereof | |
| CN102344812B (en) | Microbiological preparation for improving alkaline land, its preparation method and its application | |
| CN101538173B (en) | Method for producing microbial compound bacterial fertilizer | |
| CN102584366B (en) | Microbial organic fertilizer as well as preparation method and application thereof | |
| CN103992184B (en) | A kind of biological organic fertilizer | |
| CN101463334B (en) | Fermentation liquor composition for preparing biological organic fertilizer and preparation and use thereof | |
| CN104447095B (en) | A kind of biological activity organic medical fertilizer and its preparation method and application | |
| CN104447038A (en) | Biological organic/inorganic compound fertilizer as well as preparation method and application of fertilizer | |
| CN101941851A (en) | Technology and process for preparing biochemical humic acid by using kitchen waste | |
| CN105130685A (en) | Preparation method for organic fertilizer containing Nu-polyglutamic acid | |
| CN100497267C (en) | Process of fermenting fruit dregs and stalk to produce fulvic acid bacterial manure | |
| CN104130050B (en) | Multielement composite microbe fertilizer and its production method | |
| CN103194410B (en) | Paenibacillus mucilaginosus and method for producing compound microorganism bacterium agent by utilizing same | |
| CN105859474A (en) | Rice-straw antibacterial soil amendment and preparation method therefor | |
| CN102942420B (en) | Special microbial agent and bio-organic fertilizer for sweet cherry and preparation methods of special microbial agent | |
| CN107580818A (en) | A kind of soil conditioning and the integrated approach repaired | |
| CN104744182A (en) | Tobacco water retention biological organic fertilizer and preparation method thereof | |
| CN105646015A (en) | Composite microelement fertilizer containing chlorella and paenibacillus, preparation and applications thereof | |
| CN112573970A (en) | Solid-state compound microbial foliar fertilizer for improving saline-alkali soil and application thereof | |
| CN106380269A (en) | Soil remediation organic fertilizer for overcoming watermelon replantation obstacle, preparation method thereof and application thereof | |
| CN103351188B (en) | Preparation method of agricultural organic fertilizer by treating pure straws as raw material | |
| CN105315068A (en) | Composition for promoting crop growth and preparation method and fertilization method thereof | |
| CN108456651A (en) | A kind of composite bacteria agent of fermented stalk and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| ASS | Succession or assignment of patent right |
Owner name: SHUNDONG AGRICULTURAL UNIV. DEZHOU CHUANGDI MICROB Effective date: 20131113 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| C53 | Correction of patent of invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: Ma Hailin Inventor after: Xing Shangjun Inventor after: Liu Fangchun Inventor after: Ding Yanqin Inventor after: Du Binghai Inventor after: Liu Youdong Inventor after: Ma Bingyao Inventor after: Chen Bo Inventor before: Ma Hailin Inventor before: Xing Shangjun Inventor before: Liu Fangchun Inventor before: Ding Yanqin Inventor before: Du Binghai Inventor before: Liu Youdong Inventor before: Ma Bingyao Inventor before: Chen Bo |
|
| TA01 | Transfer of patent application right |
Effective date of registration: 20131113 Address after: 250014 Ji'nan Province, Lixia District, Shandong Province Cultural Road, No. 42 Applicant after: SHANDONG FOREST SCIENCE Research Institute Applicant after: Shandong Agricultural University Applicant after: DEZHOU CHUANGDI MICROBIAL RESOURCES CO.,LTD. Address before: 250014 Ji'nan Province, Lixia District, Shandong Province Cultural Road, No. 42 Applicant before: SHANDONG FOREST SCIENCE Research Institute |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131211 |