CN109644818A - A kind of Tree Drought Resistance growth-promoting colloid type matrix and its application - Google Patents
A kind of Tree Drought Resistance growth-promoting colloid type matrix and its application Download PDFInfo
- Publication number
- CN109644818A CN109644818A CN201910010995.2A CN201910010995A CN109644818A CN 109644818 A CN109644818 A CN 109644818A CN 201910010995 A CN201910010995 A CN 201910010995A CN 109644818 A CN109644818 A CN 109644818A
- Authority
- CN
- China
- Prior art keywords
- colloid type
- type matrix
- promoting
- drought
- tree
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention provides a kind of Tree Drought Resistance growth-promoting colloid type matrix and its using seedling medium.The Tree Drought Resistance growth-promoting colloid type matrix is mixed by solid fungicide, turf, perlite and vermiculite；Wherein, the solid fungicide is that drought resisting antimicrobial composition bacterium solution is uniformly inoculated into colloid type fermentation material to form solid fungicide；Drought resisting antimicrobial composition bacterium solution is compounded by bacillus subtilis GE3, bacillus cereus Z77 and Lactobacillus casei 1.0580.The drought resisting growth-promoting activity of bacillus subtilis GE3 can not only be given full play to, it can also be made to play the collaboration synbiosis between bacterial strain in drought environment simultaneously, washing cow dung and cow dung biogas residue mixed fermentation product is added in matrix simultaneously, it further increases microorganism and colonizes survival performance, the recycling to waste materials such as cow dungs is realized simultaneously, the value with good practical application.
Description
Technical field
The invention belongs to seedling medium and forestry microorganisms technical fields, and in particular to a kind of Tree Drought Resistance growth-promoting colloid type
Matrix and its application.
Background technique
Disclosing the information of the background technology part, it is only intended to increase understanding of the overall background of the invention, without certainty
It is considered as recognizing or implying in any form that information composition has become existing skill well known to persons skilled in the art
Art.
Matrix is the important component of nursery, it is the needs according to growth of seedling, utilizes organic and inorganic material and micro-
The artificial soil that biological agent is formulated.External seedling medium mostly uses greatly the formula of the 1960s and 1970s, such as U.S.
The composite interstitial substance A and B of Cornell University.At present, peat is best seedling medium generally acknowledged both at home and abroad, and the country is at this stage
Nursery is mostly using two parts of peat, the seedling medium of a vermiculite.This seedling medium has good educate in most plants
Seedling effect, but turf belongs to non-renewable resources, and nutrient is easy the watering with matrix and is lost, and later period nutrition is difficult to pass through work
The mode of factory is supplemented, while water demand is larger during nursery, causes biggish water resource waste.
In recent years, with milk cow production scale, intensive rapid development, caused by cattle farm excrement concentrates discharge
Problem of environmental pollution is increasingly prominent.National cow population 1218.9 ten thousand in 2007, cow dung day excretion are up to 20~300,000
T, especially large-scale milch cow farms, if lacking the means that are effectively treated, a large amount of cow dung accumulations be easy to cause serious pollution.With other
Animal wastes are compared, and cattle manure anaerobic fermentation total solids content methane production is low, and cow dung crude fiber content is more, account for dry matter percentage
Close to 40%, lignocellulose degradation is slow.It how to be efficiently one of difficult point of cultivation industry using cattle farm excrement.
One free-revving engine of forest seedling growth is exactly the surrival rate of afforestation in order to improve drought mountain.Currently, utilizing micro- life
The drought tolerance that the technological means of object improves forest becomes a new research topic.However there has been no about comprehensive ox so far
Field excrement and microbial technique prepare the report of Tree Drought Resistance growth-promoting seedling medium.
Summary of the invention
In view of the deficienciess of the prior art, the present invention provides a kind of Tree Drought Resistance growth-promoting colloid type matrix, in the matrix
By compounding a variety of different function active microorganisms, the drought resisting growth-promoting that can not only give full play to bacillus subtilis GE3 is active,
Simultaneously can also make its in drought environment play bacterial strain between collaboration synbiosis, while in matrix be added washing cow dung and
Cow dung biogas residue mixed fermentation product, further increases microorganism and colonizes survival performance, while realizing to salvage properties such as cow dungs
The recycling in source, the value with good practical application.
An object of the present invention is to provide a kind of Tree Drought Resistance growth-promoting colloid type matrix.
The second object of the present invention is to provide the application of above-mentioned Tree Drought Resistance growth-promoting colloid type matrix.
For achieving the above object, the present invention relates to following technical schemes:
The first aspect of the invention provides a kind of Tree Drought Resistance growth-promoting colloid type matrix, the Tree Drought Resistance growth-promoting glue
Figure matrix contains bacillus subtilis (Bacillus subtilis) GE3, during which has been preserved on January 7th, 2016
State's Microbiological Culture Collection administration committee common micro-organisms center (abbreviation CGMCC, address are as follows: Chaoyang District, Beijing City North Star west
The institute 3 of road 1), deposit number is CGMCC No.11966.
Bacillus subtilis (Bacillus subtilis) GE3 is that the plant rhizosphere of one plant of adaptation Drought Stress promotees
Endophytic bacteria, bacterium colony is rounded irregular on LB culture medium, and bacterium colony size is about 5mm, dry tack free tarnish side for 24 hours for culture
Edge is irregular, and bacterium colony is in brown color and intermediate color is deeper, opaque, there is gemma.
Further, the Tree Drought Resistance growth-promoting colloid type matrix contains one group of drought resisting microorganism combining bacteria, the drought resisting
Microorganism combining bacteria is compounded by bacillus subtilis GE3, bacillus cereus Z77 and Lactobacillus casei.Further
, the ratio between CFU of the bacillus subtilis GE3, bacillus cereus Z77 and Lactobacillus casei is 10:1~2:1~2；
The ratio between the CFU of three bacterial strains is determined through manure trial.
Wherein, bacillus cereus (Bacillus cereus) Z77 is preserved in China on December 31st, 2012
Microbiological Culture Collection administration committee common micro-organisms center, deposit number are CGMCC No.7070, and are recorded in Chinese special
Benefit " one plant of bacillus cereus and its improving the application in plant drought ability " is in (CN 103667132A).
The Lactobacillus casei (Lactobacillus casei) derives from Chinese microorganism strain preservation conservator
Meeting common micro-organisms center (CGMCC), can be bought by commercial sources；Further, the Lactobacillus casei preservation is compiled
Number be 1.0580.
Further, the Tree Drought Resistance growth-promoting colloid type matrix by solid fungicide, turf, perlite and vermiculite mix and
At.
Wherein, the solid fungicide is uniformly to be inoculated into drought resisting antimicrobial composition bacterium solution in colloid type fermentation material to be formed admittedly
Body microbial inoculum, inoculative proportion are colloid type fermentation material: drought resisting antimicrobial composition bacterium solution composition=5kg:1~2L (W/V)；
Wherein, drought resisting antimicrobial composition bacterium solution is by bacillus subtilis GE3, bacillus cereus Z77 and cheese lactic acid bar
Bacterium 1.0580 compounds, and the ratio between CFU of three bacterial strains is 10:1~2:1~2；Containing viable count in bacterium solution is 6~8 × 109cfu/
ml；
The preparation method of colloid type fermentation material is to mix cow dung biogas residue and washing cow dung according to 1:1 ratio, using a pile
Mixed material is carried out decomposed, pile heap height > 1m by the mode of fermentation, is wider than 3m, and length > 20m increases laggard to heap temperature
Row turning, fermentation time is 4~8d, spare after air-drying.
Further, the addition mass ratio of the solid fungicide, turf, perlite and vermiculite is 20-30:30-40:10-
15:5-10.
The second aspect of the invention provides above-mentioned Tree Drought Resistance growth-promoting colloid type matrix and is improving forest drought tolerance
Property, and then improve drought mountain afforestation survival rate and retention rate in application.
Wherein, the forest includes but is not limited to Chinese wax, arbor-vitae.
Compared with prior art, the present invention having the following effects that and advantage:
1, the present invention is using bacillus subtilis GE3 as main component, and optimum organization bacillus cereus Z77 and cheese
Bacillus acidi lactici realizes multi-functional bacterial strain combination, has given full play to the collaboration synbiosis between bacterial strain, answered in Drought Stress
With forest drought tolerance can be improved.
2, cow dung biogas residue and washing cow dung mixed fermentation are not only realized the recycling of waste material by the present invention, and
Tunning has certain gel characteristics, and good water absorption, water-retaining property is strong, is suitable for the microorganism in drought resisting antimicrobial composition microbial inoculum
Colonize survival.
3, drought resisting growth-promoting colloid type matrix of the invention, when nursery, can save moisture, reduce disease, and can after nursery stock afforestation
Water use efficiency is improved, the afforestation survival rate and retention rate of drought mountain is improved.
Detailed description of the invention
Fig. 1 is colonial morphology figure of bacillus subtilis (Bacillus subtilis) GE3 on LB culture medium flat plate；
Fig. 2 is bacillus subtilis (Bacillus subtilis) GE3 light microscopic figure；
Fig. 3 is 2 experimental group of embodiment and control group arbor-vitae container shoot survival percent and storage rate histogram.
Specific embodiment
It is noted that following detailed description is all illustrative, it is intended to provide further instruction to the application.Unless another
It indicates, all technical and scientific terms used herein has usual with the application person of an ordinary skill in the technical field
The identical meanings of understanding.
It should be noted that term used herein above is merely to describe specific embodiment, and be not intended to restricted root
According to the illustrative embodiments of the application.As used herein, unless the context clearly indicates otherwise, otherwise singular
Also it is intended to include plural form, additionally, it should be understood that, when in the present specification using term "comprising" and/or " packet
Include " when, indicate existing characteristics, step, operation, device, component and/or their combination.
In conjunction with specific example, the present invention is further illustrated, and following instance is not right merely to the explanation present invention
Its content is defined.If the experiment actual conditions being not specified in embodiment, usually according to normal condition, or it is public according to sale
Take charge of recommended condition；It is not particularly limited in the present invention, can be commercially available by commercial sources.
As background technique is introduced, in the prior art, there has been no about comprehensive cattle farm excrement and microorganism skill so far
Art prepares the report of Tree Drought Resistance growth-promoting seedling medium.
In view of this, providing a kind of Tree Drought Resistance growth-promoting colloid type matrix, institute in the specific embodiment of the present invention
It states Tree Drought Resistance growth-promoting colloid type matrix and contains bacillus subtilis (Bacillus subtilis) GE3, the bacterial strain is in 2016
It is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (abbreviation CGMCC, address are as follows: north on January 7, in
The institute 3 of the Chaoyang District Jing Shi North Star West Road 1), deposit number is CGMCC No.11966.
Bacillus subtilis (Bacillus subtilis) GE3 is that the plant rhizosphere of one plant of adaptation Drought Stress promotees
Endophytic bacteria, bacterium colony is rounded irregular on LB culture medium, and bacterium colony size is about 5mm, dry tack free tarnish side for 24 hours for culture
Edge is irregular, and bacterium colony is in brown color and intermediate color is deeper, opaque, there is gemma.
Its physiological and biochemical property is as follows:
It is as follows that its growth-promoting bacterium produces protease assay result:
The 16s rDNA sequence (see SEQ ID No.1) of bacterial strain GE3 is sequenced, and combines morphological features, life
Biochemical character is managed, bacterial strain GE3 is accredited as bacillus subtilis (Bacillus subtilis).
In still another embodiment of the invention, the Tree Drought Resistance growth-promoting colloid type matrix contains one group of micro- life of drought resisting
Object combination strain, the drought resisting microorganism combining bacteria is by bacillus subtilis GE3, bacillus cereus Z77 and Lactobacillus casei
It compounds.The ratio between CFU of the bacillus subtilis GE3, bacillus cereus Z77 and Lactobacillus casei is 10:1~2:
1~2；The ratio between the CFU of three bacterial strains is determined through manure trial.
Wherein, bacillus cereus (Bacillus cereus) Z77 is preserved in China on December 31st, 2012
Microbiological Culture Collection administration committee common micro-organisms center, deposit number are CGMCC No.7070, and are recorded in Chinese special
Benefit " one plant of bacillus cereus and its improving the application in plant drought ability " is in (CN 103667132A).
The Lactobacillus casei (Lactobacillus casei) derives from Chinese microorganism strain preservation conservator
Meeting common micro-organisms center (CGMCC), can be bought by commercial sources；
In still another embodiment of the invention, the Lactobacillus casei deposit number is 1.0580.
In still another embodiment of the invention, the Tree Drought Resistance growth-promoting colloid type matrix by solid fungicide, turf,
Perlite and vermiculite mix.
In still another embodiment of the invention, the solid fungicide is uniformly to be inoculated with drought resisting antimicrobial composition bacterium solution
Solid fungicide is formed into colloid type fermentation material, inoculative proportion is colloid type fermentation material: drought resisting antimicrobial composition bacterium solution composition
=5kg:1~2L (W/V)；
In still another embodiment of the invention, drought resisting antimicrobial composition bacterium solution is by bacillus subtilis GE3, waxy bud
Spore bacillus Z77 and Lactobacillus casei 1.0580 compound, and the ratio between CFU of three bacterial strains is 10:1~2:1~2；Contain in bacterium solution
Viable count is 6~8 × 109cfu/ml；
In still another embodiment of the invention, the preparation method of colloid type fermentation material is by cow dung biogas residue and washing ox
Excrement is mixed according to 1:1 ratio, and mixed material is carried out decomposed be made by the way of pile fermentation；Specifically, pile heap height >
1m is wider than 3m, and length > 20m carries out turning after heap temperature raising, and fermentation time is 4~8d, spare after air-drying.
In still another embodiment of the invention, the solid fungicide, turf, perlite and vermiculite addition mass ratio
For 20-30:30-40:10-15:5-10.
In still another embodiment of the invention, it is dry in raising forest to provide above-mentioned Tree Drought Resistance growth-promoting colloid type matrix
Application in the afforestation survival rate and retention rate of non-irrigated tolerance and then raising drought mountain.
Wherein, the forest includes but is not limited to Chinese wax, arbor-vitae.
Technical solution of the present invention is further elaborated combined with specific embodiments below.Wherein, cheese lactic acid in embodiment
Bacillus derives from China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), deposit number 1.0580.
Embodiment 1
It is adopted as the LKY-1 type non-woven fabrics seedling forming machine filling matrix of Shandong Forest Science Academy's development.Matrix
It is cylindrical, high 12cm, diameter 9cm after molding.5 different tests processing are set, is not inoculated with respectively microbial inoculum (CK), is inoculated with withered
Careless bacillus DZ1, bacillus subtilis GE3, bacillus cereus Z77, Lactobacillus casei and GE3, Z77 and cheese cream
The composite bacterial solution of acidfast bacilli.Wherein bacillus subtilis DZ1 is preserved in Chinese microorganism strain preservation on October 14th, 2011
Administration committee's common micro-organisms center preservation, deposit number are CGMCC No.5318.It is sowed after presprouting of seeds.Every processing
30 containers for plant growth, the 15d after emergence, final singling one in each container for plant growth.Wherein 15 nursery are measured after sowing 90d to hold
The height of seedling of Chinese wax, ground diameter and fresh weight in device.Remaining nursery stock and natural drought measure leaf r elative water content and relatively electric after 20d
Conductance.As a result it is as shown in the table.As can be seen that the promotion Chinese wax growth that composite bacterial solution is different degrees of, and it is opposite to improve blade
Water content reduces blade relative conductivity, this illustrates that composite bacterial solution to nursery tree growth is promoted, improves drought resistance of seedlings tool
There is positive effect.
The relative water content and relative conductivity of 1 Chinese wax growing way of table and blade
| Processing | Height of seedling/cm | Ground diameter/mm | Total fresh weight/g | Leaf r elative water content (%) | Blade relative conductivity (%) |
| DZ1 | 68.54±2.54 | 5.13±0.23 | 38.64±1.75 | 76.53±2.53 | 57.35±2.36 |
| GE3 | 71.66±2.42 | 5.94±0.13 | 41.77±1.32 | 80.54±2.66 | 54.26±1.89 |
| Z77 | 69.17±1.96 | 5.44±0.19 | 39.13±1.63 | 76.28±1.85 | 58.63±3.26 |
| Lactobacillus casei | 67.67±2.37 | 5.04±0.20 | 39.04±1.23 | 75.96±1.93 | 56.93±2.14 |
| Composite bacterial solution | 73.66±2.17 | 6.72±0.17 | 43.37±1.25 | 82.15±2.74 | 52.17±2.81 |
Embodiment 2
Arbor-vitae container seedling is cultivated using drought resisting growth-promoting colloid type matrix of the invention and conventional vessel seedling medium.In Laiwu
The Beishan Mountain Niu Wangquan of city, the town Miao Shan, the Laicheng district mountain village Bei Miao arranges afforestation experiment, in arrangement on July 12nd, 2016 afforestation experiment.It makes
The arbor-vitae container seedling that woods nursery stock selects drought resisting growth-promoting colloid type matrix and conventional vessel seedling medium of the invention to cultivate respectively.It makes
Woods current year investigation survival rate, and survival rate is investigated in next year, as a result as shown in Figure 3.As can be seen that the seedling of matrix breeding of the present invention
Wood afforestation, afforestation survival rate and retention rate, which has, is significantly higher than conventional seedbed system matrix.
It should be noted that above example is only used to illustrate the technical scheme of the present invention rather than is limited.Although ginseng
It is described the invention in detail according to given example, but those skilled in the art can be as needed to this hair
Bright technical solution is modified or replaced equivalently, without departing from the spirit and scope of the technical solution of the present invention.
SEQUENCE LISTING
<110>Shandong Forest Science Academy
<120>a kind of Tree Drought Resistance growth-promoting colloid type matrix and its application
<130>
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 1417
<212> DNA
<213>artificial synthesized
<400> 1
gcggctggct cctaaaaggt tacctcaccg acttcgggtg ttacaaactc tcgtggtgtg 60
acgggcggtg tgtacaaggc ccgggaacgt attcaccgcg gcatgctgat ccgcgattac 120
tagcgattcc agcttcacgc agtcgagttg cagactgcga tccgaactga gaacagattt 180
gtgggattgg cttaacctcg cggtttcgct gccctttgtt ctgtccattg tagcacgtgt 240
gtagcccagg tcataagggg catgatgatt tgacgtcatc cccaccttcc tccggtttgt 300
caccggcagt caccttagag tgcccaactg aatgctggca actaagatca agggttgcgc 360
tcgttgcggg acttaaccca acatctcacg acacgagctg acgacaacca tgcaccacct 420
gtcactctgc ccccgaaggg gacgtcctat ctctaggatt gtcagaggat gtcaagacct 480
ggtaaggttc ttcgcgttgc ttcgaattaa accacatgct ccaccgcttg tgcgggcccc 540
cgtcaattcc tttgagtttc agtcttgcga ccgtactccc caggcggagt gcttaatgcg 600
ttagctgcag cactaagggg cggaaacccc ctaacactta gcactcatcg tttacggcgt 660
ggactaccag ggtatctaat cctgttcgct ccccacgctt tcgctcctca gcgtcagtta 720
cagaccagag agtcgccttc gccactggtg ttcctccaca tctctacgca tttcaccgct 780
acacgtggaa ttccactctc ctcttctgca ctcaagttcc ccagtttcca atgaccctcc 840
ccggttgagc cgggggcttt cacatcagac ttaagaaacc gcctgcgagc cctttacgcc 900
caataattcc ggacaacgct tgccacctac gtattaccgc ggctgctggc acgtagttag 960
ccgtggcttt ctggttaggt accgtcaagg taccgcccta ttcgaacggt acttgttctt 1020
ccctaacaac agagctttac gatccgaaaa ccttcatcac tcacgcggcg ttgctccgtc 1080
agactttcgt ccattgcgga agattcccta ctgctgcctc ccgtaggagt ctgggccgtg 1140
tctcagtccc agtgtggccg atcaccctct caggtcggct acgcatcgtt gccttggtga 1200
gccgttacct caccaactag ctaatgcgcc gcgggtccat ctgtaagtgg tagccgaagc 1260
caccttttat gtttgaacca tgcggttcaa acaaccatcc ggtattagcc ccggtttccc 1320
ggagttatcc cagtcttaca ggcaggttac ccacgtgtta ctcacccgtc cgccgctaac 1380
atcagggagc aagctcccat ctgtccgctc gactgca 1417
Claims (10)
1. a kind of Tree Drought Resistance growth-promoting colloid type matrix, which is characterized in that the Tree Drought Resistance growth-promoting colloid type matrix contains withered
Careless bacillus (Bacillus subtilis) GE3, the bacterial strain have been preserved in Chinese microorganism strain guarantor on January 7th, 2016
Administration committee's common micro-organisms center is hidden, deposit number is CGMCC No.11966.
2. a kind of Tree Drought Resistance growth-promoting colloid type matrix as described in claim 1, which is characterized in that the Tree Drought Resistance growth-promoting
Colloid type matrix contains one group of drought resisting microorganism combining bacteria, and the drought resisting microorganism combining bacteria is by bacillus subtilis GE3, waxy
Bacillus Z77 and Lactobacillus casei compound.
3. a kind of Tree Drought Resistance growth-promoting colloid type matrix as claimed in claim 2, which is characterized in that the bacillus subtilis
The ratio between CFU of GE3, bacillus cereus Z77 and Lactobacillus casei is 10:1~2:1~2.
4. a kind of Tree Drought Resistance growth-promoting colloid type matrix as claimed in claim 2, which is characterized in that the bacillus cereus
(Bacillus cereus) Z77 has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and preservation is compiled
Number be CGMCC No.7070.
5. a kind of Tree Drought Resistance growth-promoting colloid type matrix as claimed in claim 2, which is characterized in that it is characterized in that, described
Lactobacillus casei (Lactobacillus casei) derives from China Committee for Culture Collection of Microorganisms's common micro-organisms
Center, it is preferred that the Lactobacillus casei deposit number is 1.0580.
6. a kind of Tree Drought Resistance growth-promoting colloid type matrix, which is characterized in that it is mixed by solid fungicide, turf, perlite and vermiculite
It is even to form；
Wherein, the solid fungicide is that drought resisting antimicrobial composition bacterium solution is uniformly inoculated into colloid type fermentation material to form solid bacterium
Agent；
Drought resisting antimicrobial composition bacterium solution is answered by bacillus subtilis GE3, bacillus cereus Z77 and Lactobacillus casei 1.0580
With forming.
7. Tree Drought Resistance growth-promoting colloid type matrix as claimed in claim 6, which is characterized in that inoculative proportion is colloid type fermentation
Material: drought resisting antimicrobial composition bacterium solution composition=5kg:1~2L；
The ratio between CFU of bacillus subtilis GE3, bacillus cereus Z77 and Lactobacillus casei 1.0580 be 10:1~2:1~
2；Containing viable count in bacterium solution is 6~8 × 109cfu/ml。
8. Tree Drought Resistance growth-promoting colloid type matrix as claimed in claims 6 or 7, which is characterized in that the system of colloid type fermentation material
Preparation Method is to mix cow dung biogas residue and washing cow dung according to 1:1 ratio, is carried out mixed material by the way of pile fermentation
It is decomposed to be made；
Preferably, the addition mass ratio of the solid fungicide, turf, perlite and vermiculite is 20-30:30-40:10-15:5-
10。
9. any one of the claim 1-8 Tree Drought Resistance growth-promoting colloid type matrix is improving forest drought tolerance, Jin Erti
Application in the afforestation survival rate and retention rate of high drought mountain.
10. application as claimed in claim 9, which is characterized in that the forest includes Chinese wax, arbor-vitae.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910010995.2A CN109644818B (en) | 2019-01-07 | 2019-01-07 | Drought-resistant growth-promoting colloidal matrix for forest trees and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910010995.2A CN109644818B (en) | 2019-01-07 | 2019-01-07 | Drought-resistant growth-promoting colloidal matrix for forest trees and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109644818A true CN109644818A (en) | 2019-04-19 |
| CN109644818B CN109644818B (en) | 2020-12-29 |
Family
ID=66118912
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910010995.2A Active CN109644818B (en) | 2019-01-07 | 2019-01-07 | Drought-resistant growth-promoting colloidal matrix for forest trees and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109644818B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115777489A (en) * | 2022-11-23 | 2023-03-14 | 山东省林业科学研究院 | Biological greening method for garden engineering |
| CN117069544A (en) * | 2023-08-21 | 2023-11-17 | 山东省林业科学研究院 | Method for improving water fertilizer utilization and drought resistance of poplar in sandy soil |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102627504A (en) * | 2012-04-20 | 2012-08-08 | 山东光大肥业科技有限公司 | Culture substrate special for organic herbal fruits and preparation method thereof |
| CN103609397A (en) * | 2013-10-12 | 2014-03-05 | 江苏徐淮地区淮阴农业科学研究所 | Seedling-cultivating substrate capable of preventing and treating soil-borne diseases and production method thereof |
| CN103650977A (en) * | 2013-12-06 | 2014-03-26 | 山东省林业科学研究院 | Method for improving survival rate of afforestation of non-woven fabric container seedlings in arid mountains |
| CN104745514A (en) * | 2015-04-01 | 2015-07-01 | 山东省林业科学研究院 | Blueberry rhizosphere growth-promoting bacillus cereus as well as composite microbial agent and application thereof |
| CN104844311A (en) * | 2015-03-31 | 2015-08-19 | 山东省林业科学研究院 | Substrate and method for improving survival rate of afforestation in drought and barren mountainous regions |
| CN105198583A (en) * | 2015-09-24 | 2015-12-30 | 临汾市力澜生物科技有限公司 | Nutrient medium for soilless culture |
| CN107056487A (en) * | 2017-05-26 | 2017-08-18 | 北京工商大学 | A kind of soil conditioner and preparation method thereof |
| CN107384840A (en) * | 2017-09-07 | 2017-11-24 | 北京农业生物技术研究中心 | One plant of drought resisting growth-promoting composite bacteria agent and its application |
| CN108668830A (en) * | 2018-04-04 | 2018-10-19 | 苏斌 | A kind of disease-resistant pest-resistant organic vegetable planting matrix |
-
2019
- 2019-01-07 CN CN201910010995.2A patent/CN109644818B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102627504A (en) * | 2012-04-20 | 2012-08-08 | 山东光大肥业科技有限公司 | Culture substrate special for organic herbal fruits and preparation method thereof |
| CN103609397A (en) * | 2013-10-12 | 2014-03-05 | 江苏徐淮地区淮阴农业科学研究所 | Seedling-cultivating substrate capable of preventing and treating soil-borne diseases and production method thereof |
| CN103650977A (en) * | 2013-12-06 | 2014-03-26 | 山东省林业科学研究院 | Method for improving survival rate of afforestation of non-woven fabric container seedlings in arid mountains |
| CN104844311A (en) * | 2015-03-31 | 2015-08-19 | 山东省林业科学研究院 | Substrate and method for improving survival rate of afforestation in drought and barren mountainous regions |
| CN104745514A (en) * | 2015-04-01 | 2015-07-01 | 山东省林业科学研究院 | Blueberry rhizosphere growth-promoting bacillus cereus as well as composite microbial agent and application thereof |
| CN105198583A (en) * | 2015-09-24 | 2015-12-30 | 临汾市力澜生物科技有限公司 | Nutrient medium for soilless culture |
| CN107056487A (en) * | 2017-05-26 | 2017-08-18 | 北京工商大学 | A kind of soil conditioner and preparation method thereof |
| CN107384840A (en) * | 2017-09-07 | 2017-11-24 | 北京农业生物技术研究中心 | One plant of drought resisting growth-promoting composite bacteria agent and its application |
| CN108668830A (en) * | 2018-04-04 | 2018-10-19 | 苏斌 | A kind of disease-resistant pest-resistant organic vegetable planting matrix |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115777489A (en) * | 2022-11-23 | 2023-03-14 | 山东省林业科学研究院 | Biological greening method for garden engineering |
| CN115777489B (en) * | 2022-11-23 | 2023-06-02 | 山东省林业科学研究院 | Garden engineering biological greening method |
| CN117069544A (en) * | 2023-08-21 | 2023-11-17 | 山东省林业科学研究院 | Method for improving water fertilizer utilization and drought resistance of poplar in sandy soil |
| CN117069544B (en) * | 2023-08-21 | 2024-05-10 | 山东省林业科学研究院 | Method for improving water fertilizer utilization and drought resistance of poplar in sandy soil |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109644818B (en) | 2020-12-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104789226B (en) | Charcoal base microbe soil conditioner and preparation method thereof | |
| CN102199562B (en) | Composite bacterial preparation as well as preparation method and application thereof | |
| CN100425690C (en) | Fast harmless biological treating process of animal excrement and manure water | |
| CN102286376B (en) | Microbial inoculum for high-efficiency fermenting bed and preparation method thereof | |
| CN103194407B (en) | Straw-decomposition composite microbial preparation and preparation method thereof | |
| CN105524858B (en) | A kind of high temperature resistant decomposing microbial inoculum of organic waste and its application | |
| CN110577911B (en) | Bacillus pumilus and application thereof | |
| CN109762765B (en) | Decomposed solid fermentation microbial inoculum and application thereof in agricultural wastes | |
| CN103484396B (en) | New strain of streptomyces thermocarboxydus and application thereof | |
| CN101880182A (en) | Low-temperature fast fermentation agent for animal dung in cold regions as well as preparation method and application thereof | |
| CN107176891A (en) | It is a kind of to promote the biological agent and its production technology of stalk fast degradation | |
| CN109762767B (en) | Sewage composite microbial treatment agent and preparation method and application thereof | |
| CN105543149B (en) | One plant of new bacillus megaterium and its application | |
| CN108018233A (en) | One plant of Siam bacillus and its preparation and application of microbial bacterial agent | |
| CN105754888B (en) | Bacillus licheniformis and microbial bacterial agent and their applications in fermentation bed cultivation | |
| CN109644818A (en) | A kind of Tree Drought Resistance growth-promoting colloid type matrix and its application | |
| CN103074228B (en) | Composite fermentation inoculant for processing animal wastes, and preparation method and application thereof | |
| CN107628894A (en) | Composite bacteria agent increase soil fertility and its preparation method and application | |
| CN102598978A (en) | Method for improving chromium resistance and cadmium resistance of Festuca arundinacea with compost fermentation liquid | |
| CN102598977B (en) | Method for improving chromium resistance and cadmium resistance of ryegrass with compost fermentation liquid | |
| CN101993832A (en) | Composite bacterial agent for pig manure compost treatment and application method | |
| CN103497925B (en) | The genetic engineering bacterium KT-puts2 of one strain degraded sulfonylurea herbicide and application thereof | |
| CN113025507B (en) | Compound microbial agent, preparation thereof and application thereof in deodorization and purification of high-concentration wastewater in livestock and poultry farms | |
| CN114045232B (en) | Organic material rapid fermentation synergistic microbial agent and application thereof | |
| CN107788230A (en) | It is a kind of to improve earthworm growth rate and the cultivation matrix of reproductive performance |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |