CN104789226B - Charcoal base microbe soil conditioner and preparation method thereof - Google Patents

Charcoal base microbe soil conditioner and preparation method thereof Download PDF

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CN104789226B
CN104789226B CN201510111933.2A CN201510111933A CN104789226B CN 104789226 B CN104789226 B CN 104789226B CN 201510111933 A CN201510111933 A CN 201510111933A CN 104789226 B CN104789226 B CN 104789226B
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CN104789226A (en
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陈家辉
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Disco Technology Group (Yichang) Limited
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    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K17/00Soil-conditioning materials or soil-stabilising materials
    • C09K17/40Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K2101/00Agricultural use
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
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Abstract

The invention discloses a kind of charcoal base microbe soil conditioner, it is made of following component:80~85 parts by weight charcoals, 10~13 parts by weight complex microbial communities and 1~2 parts by weight oxidized starch adhesive, strain includes in complex microbial community:Photosynthetic bacteria, actinomyces, lactic acid bacteria, saccharomycete, bacillus subtilis and bacillus thuringiensis.The invention also discloses the preparation method of this charcoal base microbe soil conditioner.It alleviates soil acidification, reduces the soil weight, increase soil permeability and water content by the synergy of charcoal base and microorganism;By promoting the soil organism to be formed, the validity of mineral element is improved;By cultivating the abundance of rhizosphere microorganisms of crops, improve the rhizospheric environment of plant, pathogenic microorganism is difficult to growth and breeding so as to reduce the generation of crop disease and insect, slow down and polluted with repairing heavy metal in soil.

Description

Charcoal base microbe soil conditioner and preparation method thereof
Technical field
The present invention relates to agricultural fertilizer, particularly a kind of charcoal base microbe soil conditioner, the invention further relates to this The preparation method of kind charcoal base microbe soil conditioner.
Background technology
2014《Chinese arable land credit rating is investigated and evaluation》The result shows that China excellently, highly, mediumly and Low ground area accounts for 2.9%, 26.5%, 52.9% and the 17.7% of the whole nation arable land evaluation gross area respectively.Wherein, in, it is low Ground cultivated area accounts for the 70.6% of arable land evaluation area, illustrates that China's farmland quality is totally relatively low.Comprehensive research result also table Bright, Chinese arable land the overall level of quality tends to decline.Zhao Qiguo thinks that the soil quality of Chinese arable land is on a declining curve:The whole nation is ploughed The ground content of organic matter averagely has dropped down to 1%, hence it is evident that less than the level of American-European countries 2.5~4.0%.Remained shock state points out Chinese arable land Quality declines main cause:First, non-agricultural construction covers good farmland, and open up wasteland compensation is then ground inferior, causes total constitution of ploughing Amount declines;Second, arable land " use ", " foster " are improper, farmland quality is caused to decline;Third, cultivated land degradation aggravation causes farmland quality not It is disconnected to decline;Fourth, arable land property right system imperfection, management is not in place, and it is pre- to manage no long-term, good profit of ploughing Phase, causes predation formula to utilize or arbitrarily let a piece of farmland go to waste and exacerbates the decline of farmland quality.Meanwhile industrial wastewater, waste residue, exhaust gas are largely arranged Put and mechanization of agriculture, the unreasonable use of pesticide, chemical fertilizer, agricultural film, so as to cause soil pollution, soil hardening, pesticide is residual Serious, the problems such as agricultural non-point source pollution expands is stayed, these have been further exacerbated by the decline of soil quality.
China's biology carbon feedstock is sufficient, and stalk resource is extremely abundant.The utilization of stalk resource, had both been related to agriculture life Material Efficient Conversion and energy efficient circulation in production system, become the important realization means of circular agriculture, also relate to whole Soil fertility, water and soil conservation, Environmental security and renewable resource in Agro-ecological System such as efficiently utilize at the sustainable development Problem, is China's planting industry and the important substance of aquaculture sustainable development and rural novel energy basis.Stalk resource Quantity survey (surveying) is the basis of stalk resource comprehensive utilization.The result of study of Wang Xiao jade 2008-2010 shows that East China is average annual Stalk total amount is 199.5 million tons, and the average annual stalk total amount in ALFISOL IN CENTRAL is 213.9 million tons, the field crop year of southwest Equal 93.3 million tons of stalk total amount.Finish in cloud result of study it is further noted that vegetable byproducts i.e. vegetables tendril and residue with And the stock number such as agricultural product primary processed side product rice husk, corncob, bagasse, megasse is also quite huge, stalk money is enriched The available scope in source.
Charcoal is that a kind of biomass height aromatization that thermal cracking produces in the case of completely or partially anoxic is difficult Dissolubility solid matter.Charcoal has soil good improving effect, and for most of charcoal in alkalescence, acid can be improved by being manured into soil Property soil pH value.Charcoal has complicated multi-pore structure and larger specific surface area, and being manured into soil, it is penetrating to increase soil Property, improve soil agreegate, adsorb more moisture and nutrient ions, improve soil water capacity and nutrient sticking capacity.Biology Contain a certain amount of easy decomposing organic compounds in matter charcoal, edaphon can be as carbon source, it is possible to increase soil is micro- The biomass and activity of biology, biomass carbon have complicated pore structure, can be as microorganism and its load of available nutrient Body, is conducive to Soil Microorganism community growth.
Biomass carbon has complicated pore structure, can be conducive to soil as microorganism and its carrier of available nutrient Rang Zhong microbiologic populations grow.
Photosynthetic bacteria is prevalent in nature, has the prokaryotes of luminous energy synthetic system, is that one kind is not formed The Gram-negative bacteria of gemma ability, be one kind using light as the energy, can under anaerobism illumination or aerobic dark condition utilize oneself Organic matter, sulfide, ammonia in right boundary etc. carry out photosynthetic microorganism as hydrogen donor and carbon source.It can promote animals and plants Growth, improves premunition, plays a role to animals and plants vital movement and growth and development.
Actinomyces are a kind of mainly in mycelial growth and with the more powerful prokaryotes of the terrestrial of sporogenesis.Leather is blue Family name is positive, is the important producer of some row bioactive substances such as antibiotic, has in terms of diseases and pests of agronomic crop biological control Play an important role.Can be with a variety of useful metabolites such as synthetic starch enzyme, cellulase, amino acid urgency vitamins.In soil Promote Zinc fractions crumb structure in earth so as to improve soil.
Lactobacillus-fermented carbohydrate primary product is a kind of general name without gemma, gram-positive bacterium of lactic acid.For Prokaryotes.Aerobic respiration is carried out using sugar under aerobic conditions, energy is obtained during sugar is decomposed, synthetic cell material, In anaerobic environment, the growth of corruptibility microorganism in growing environment can be suppressed by producing lactic acid.
Saccharomycete is some unicellular fungis, and aerobe, is important nutrient functional bacterium, can to including soil, Available nutrient carries out rational conversion and efficient absorption in the various matrix environments such as water body;Also ferment decomposition, A variety of B family vitamins and physiological activator can be formed in growth metabolism, strengthens the activity of effective bacterium.
Bacillus subtilis is one kind of Bacillus, is widely distributed in soil and the organic matter of corruption, easily withered Bred in grass leaching juice.Subtilin, polymyxins, nystatin, the gramicidins isoreactivity thing produced during thalli growth Matter, these active materials have obvious inhibitory action to the conditioned pathogen of pathogenic bacteria or autogenous infection.
Bacillus thuringiensis abbreviation Bt, is a kind of production crystal bacillus for including many mutation.Be current production rate most Greatly, using most wide biological insecticides.Its main active is one or several kinds of insecticidal crystal proteins, also known as δ-endogenous toxic material Element, to insects such as Lepidoptera, coleoptera, Diptera, Hymenoptera, Homopteras, and the arthropod such as animals and plants nematode, acarian is all Have a specific cytotoxicity, and to nontarget organism safety therefore, Bt insecticides have it is single-minded, efficiently and to safety of human and livestock etc. Advantage.
The content of the invention
The first object of the present invention is the shortcoming for overcoming above-mentioned existing background technology, and provides a kind of charcoal base Microbe soil conditioner.
The second object of the present invention is the preparation method for providing this charcoal base microbe soil conditioner.
The first object of the present invention is reached by following measure:Charcoal base microbe soil conditioner, by such as Lower component composition:80~85 parts by weight charcoals, 10~13 parts by weight complex microbial communities and 1~2 parts by weight oxidized starch Adhesive;
Strain is by photosynthetic bacteria, actinomyces, lactic acid bacteria, saccharomycete, bacillus subtilis and Soviet Union in complex microbial community Cloud gold bacillus forms.The content of each strain is respectively:Photosynthetic bacteria content is not low not less than 10,000,000,000/ml, unwrapping wire bacterial content In 15,000,000,000/ml, content of lactic acid bacteria not less than 15,000,000,000/ml, yeast bacterial content not less than 2,000,000,000/ml, bacillus subtilis bacterial content It is not less than 2,000,000,000/ml not less than 2,000,000,000/ml and bacillus thuringiensis content;
The viscosity of oxidized starch adhesive is 25~30mPa ﹒ s, and the oxidized starch adhesive is by 100~150 parts by weight Starch, 3~5 weight account polyethylene alcohol, 1~3 parts by weight glycerine, 8~10 parts by weight, 30% hydrogen peroxide, 1~2 parts by weight boron Sand forms.
In the above-mentioned technical solutions, charcoal base microbe soil conditioner, is preferably, is made of following component:85 weights Measure part charcoal, 13 parts by weight complex microbial communities and 2 parts by weight oxidized starch adhesives;
The oxidized starch adhesive is preferably that viscosity is 30mPa ﹒ s, and the oxidized starch adhesive is by 150 weight Part starch, 3.5 weight account polyethylene alcohol, 2 parts by weight glycerine, 9 parts by weight, 30% hydrogen peroxide and 2 parts by weight boraxs composition.
The second object of the present invention is reached by following measure:Prepare charcoal base microbe soil conditioner Method, includes the following steps:
Step 1:Prepare charcoal
Step 1.1:By stalk in air-drying processing under natural conditions, it is spare to be all milled to particle diameter 2mm with pulverizer, obtains Stalk;
Step 1.2:By the stalk after being ground in step 1.1, carbonizing apparatus hopper is sent into by conveyer belt, and utilize Raw material is sent to progress fertilizer separation in the separator on retort top by high pressure positive blower, and raw material puts live charcoal after entering retort Change, carbonization temperature is 500 DEG C, and heating rate is 5 DEG C/min, when charing 2.5~3 is small, up to charcoal;
Step 2:Prepare complex microbial community
Step 2.1:Prepare single bacterium solution:
Prepare photosynthetic bacteria liquid:The cultured photosynthetic bacteria slant strains of picking are inoculated into by 1000 parts by weight water, 3 ~5 parts by weight yeast extracts, 2~3 parts by weight acetic acid sodium, 0.5~1 parts by weight sal-ammoniac, 0.5~1 parts by weight of phosphoric acid potassium dihydrogen and On the culture medium of 0.3~0.5 parts sulfuric acid magnesium composition, culture, obtains photosynthetic bacteria liquid;
Prepare actinomyces bacterium solution:The cultured actinomyces slant strains of picking are inoculated into by 1000 parts by weight water, 15~20 Parts by weight soluble starch, 0.5~1 parts by weight, three water dipotassium hydrogen phosphate, 0.5~1 parts by wt NaCl, 0.5~1 parts by weight seven On the culture medium of water magnesium sulfate, 0.5~1 parts by weight ferrous sulfate heptahydrate and 1~2 parts by weight of potassium nitrate composition, culture, is put Line bacterium bacterium solution;
Prepare lactic acid bacterial liquid:The cultured lactic acid bacteria slant strains of picking are inoculated into by 1000 parts by weight water, 5~10 Parts by weight peptone, 5~10 parts by weight beef extracts, 3~5 parts by weight yeast extracts, 15~20 parts by weight glucose, 1~2 parts by weight Lemon acid diamine, 3~5 parts by weight sodium acetates, 1~2 parts by weight Tween 80,0.3~0.5 parts by weight epsom salt, 0.1~ On the culture medium of 0.3 parts by weight, four water manganese sulfate and 1~2 parts by weight of phosphoric acid hydrogen dipotassium composition, culture, obtains lactic acid bacterial liquid;
Prepare saccharomycete bacterium solution:The cultured saccharomycete slant strains of picking are inoculated into by 1000 parts by weight water, 0.5~1 Parts by weight dusty yeast, 15~20 parts by weight glucose, 3~5 parts sulfuric acid ammonia, 1~2 parts by weight of phosphoric acid potassium dihydrogen and 0.5~1 On the culture medium of parts by weight epsom salt composition, culture, obtains saccharomycete bacterium solution;
Prepare bacillus subtilis and bacillus thuringiensis bacterium solution:The cultured bacillus subtilis of picking and Su Yunjin Bacillus slant strains are inoculated into by 1000 parts by weight water, 10~15 parts by weight peptones, 15~20 parts by weight glucose, 3 On the culture medium of~5 parts by wt NaCl and 1~2 parts by weight of phosphoric acid potassium dihydrogen composition, culture, obtains bacillus subtilis and Soviet Union Cloud gold bacillus bacterium solution;
Step 2.2:Prepare complex microbial community:First by fermentation cylinder for fermentation culture medium and fermentation tank in 121 DEG C of sterilizings 20min, then photosynthetic bacteria liquid, actinomyces bacterium solution, lactic acid bacterial liquid, saccharomycete bacterium solution, withered grass gemma will be obtained in step 2.1 Bacillus and bacillus thuringiensis bacterium solution are inoculated into fermentation tank according to percentage by weight for 0.5%~1% inoculum concentration respectively to be sent out Ferment culture, up to complex microbial community;
Step 3:Prepare charcoal base microbe soil conditioner:Using comminutor to 80~85 parts by weight charcoals into Row is granulated, and is then sprayed into comminutor 10~13 parts by weight complex microorganism bacterium solutions with sprayer, is made charcoal to compound micro- Biological bacterium solution is fully adsorbed, while adds 1~2 parts by weight oxidized starch adhesive, up to charcoal base microbe soil improvement Agent.
In the above-mentioned technical solutions, in step 2.2 fermentation cylinder for fermentation culture medium by 1000 parts by weight water, 20~25 weight Part corn flour, 5~25 parts by weight bean powderes, 10~15 parts by weight glucose, 3~5 parts by weight of phosphoric acid hydrogen dipotassiums, 0.5~1 parts by weight Magnesium sulfate and 0.1~0.2 weight parts of calcium carbonate composition.
Fermentation cylinder for fermentation culture medium is preferably by 1000 parts by weight water, 22 parts by weight Corn powder, 20 weight in step 2.2 Part bean powder, 15 parts by weight glucose, 3 parts by weight of phosphoric acid hydrogen dipotassiums, 0.5 parts sulfuric acid magnesium and 0.1 weight parts of calcium carbonate composition.
In the above-mentioned technical solutions, fermentation temperature is 35~38 DEG C in step 2.2, and fermentation pH controls are 6.0~7.0.
The advantage of the invention is that:First, the improving effect to soil physical properties:Containing very in the ash content of charcoal base More base cations such as calcium, magnesium, potassium, sodium etc., can exchange reduces soil hydrogen ion level, so as to dramatically increase the pH of soil Value.Meanwhile soil organic carbon can be improved, beneficial to the formation of soil agreegate, larger porosity and specific surface area are formed, The soil weight is reduced, increases soil aeration and water-holding capacity.
Second, the improving effect to soil chemical properties:Charcoal base can adsorb soil organic molecule, be urged by surface Changing activity promotes small organic molecule to polymerize to form the soil organism.Charcoal is transformed into by humus by microbial action Carbon, beneficial to the formation of humus united.There is the basic body of charcoal exhibiting high surface negative electrical charge and high charge density can increase The absorption of strong soil ion exchange capacity and increase to nutrient in soil, drastically increases the adsorption capacity and nutrient of nutrient Validity, reduces Nutrient Leaching, improves fertilizer nutrient utilization.Microorganism species can accelerate the decomposition of soil organic matter to turn Change, increase soil organic nutrient and fertility.
Third, the improving effect to edaphon colony:Edaphon abundance is improved, especially plant rhizosphere is micro- Biotic component is enriched, and improves biological activity of soil and buffer capacity.Contain vitamin, plant growth in microorganism secretion material Plant growth is greatly facilitated in hormone etc..Bacillus subtilis and bacillus thuringiensis form advantage life in crop root Thing flora, its energy metabolism secreting bacteria element, lipopeptide compound, organic acid substance etc. in growth course, these metabolism productions Thing can effectively suppress growth or the dissolving pathogen of pathogen, so that killing germ, highly resistance continuous cropping.Greatly reduce pest and disease damage hair It is raw.
Fourth, the improving effect to soil environment:By the way that charcoal base is stored in soil, it can conscientiously lock and reduce Atmospheric CO2Concentration, part carbon in biomass is fixed in soil, so as to form carbon base in soil inert fraction, is stored And remain in soil, form soil carbon sink.The administration of charcoal base can significantly affect the form of heavy metal in soil and move Divide a word with a hyphen at the end of a line as charcoal can reduce the Acid extractable content of Pb, Cd in soil, thus reduce the biological effectiveness of heavy metal, right Heavy metal shows good fixed effect.
Brief description of the drawings
Fig. 1 is not add 5 charcoal base microbe soil conditioner (T of the embodiment of the present invention1), addition the embodiment of the present invention 5 charcoal base microbe soil conditioners and dry ground mass ratio are 1% (T2), addition the 5 micro- life of charcoal base of the embodiment of the present invention Thing soil conditioner and dry ground mass ratio are 2% (T3) and addition 5 charcoal base microbe soil conditioner of the embodiment of the present invention It is 4% (T with dry ground mass ratio4) influence comparison diagram to the soil weight, porosity and water content;
Fig. 2 is not add 5 charcoal base microbe soil conditioner (T of the embodiment of the present invention1), addition the embodiment of the present invention 5 charcoal base microbe soil conditioner 1thm-2(T2), addition 5 charcoal base microbe soil improvement of the embodiment of the present invention Agent 5thm-2(T3) and 5 charcoal base microbe soil conditioner 10thm of the addition embodiment of the present invention-2(T4) to soil without The influence comparison diagram of machine nitrogen content, soil organic matter content and yield.
Embodiment
With reference to embodiment and the attached drawing performance that the present invention will be described in detail, but they are not formed to the present invention's Limit, it is only for example.At the same time by illustrating that advantages of the present invention will become clearer and be readily appreciated that.
Embodiment 1
Charcoal base microbe soil conditioner, is made of following component:80 parts by weight charcoals, 10 parts by weight are compound micro- Biological flora and 2 parts by weight oxidized starch adhesives;
Strain includes in complex microbial community:Photosynthetic bacteria, actinomyces, lactic acid bacteria, saccharomycete, bacillus subtilis and Bacillus thuringiensis, the content of each strain are respectively:Photosynthetic bacteria content is not less than not less than 10,000,000,000/ml, unwrapping wire bacterial content 15000000000/ml, content of lactic acid bacteria are not less than 15,000,000,000/ml, yeast bacterial content not less than 2,000,000,000/ml, bacillus subtilis bacterial content not It is not less than 2,000,000,000/ml less than 2,000,000,000/ml and bacillus thuringiensis content;
The viscosity of oxidized starch adhesive is 25mPa ﹒ s, and the oxidized starch adhesive is by 100 weight starch, 3 weights Measure part polyvinyl alcohol, 1 parts by weight glycerine, 8 parts by weight, 30% hydrogen peroxide, 1 parts by weight borax composition.
The method for preparing charcoal base microbe soil conditioner, includes the following steps:
Step 1:Prepare charcoal
Step 1.1:By stalk in air-drying processing under natural conditions, it is spare to be all milled to particle diameter 2mm with pulverizer, obtains Stalk;
Step 1.2:By the stalk after being ground in step 1.1, carbonizing apparatus hopper is sent into by conveyer belt, and utilize Raw material is sent to progress fertilizer separation in the separator on retort top by high pressure positive blower, and raw material puts live charcoal after entering retort Change, carbonization temperature is 500 DEG C, and heating rate is 5 DEG C/min, when charing 2.5 is small, up to charcoal;
Step 2:Prepare complex microbial community
Step 2.1:Prepare single bacterium solution:
Prepare photosynthetic bacteria liquid:The cultured photosynthetic bacteria slant strains of picking are inoculated into by 1000 parts by weight water, 3 Parts by weight yeast extract, 2 parts by weight acetic acid sodium, 0.5 parts by weight sal-ammoniac, 0.5 parts by weight of phosphoric acid potassium dihydrogen and 0.3 parts sulfuric acid On the culture medium of magnesium composition, culture, obtains photosynthetic bacteria liquid;
Prepare actinomyces bacterium solution:The cultured actinomyces slant strains of picking are inoculated into by 1000 parts by weight water, 15 weight Part soluble starch, 0.5 parts by weight, three water dipotassium hydrogen phosphate, 0.5 parts by wt NaCl, 0.5 parts by weight epsom salt, 0.5 On the culture medium of parts by weight ferrous sulfate heptahydrate and 1 parts by weight of potassium nitrate composition, culture, obtains actinomyces bacterium solution;
Prepare lactic acid bacterial liquid:The cultured lactic acid bacteria slant strains of picking are inoculated into by 1000 parts by weight water, 5 weight Part peptone, 5 parts by weight beef extracts, 3 parts by weight yeast extracts, 15 parts by weight glucose, 1 parts by weight of lemon acid diamine, 3 weight Part sodium acetate, 1 parts by weight Tween 80,0.3 parts by weight epsom salt, 0.1 parts by weight, four water manganese sulfate and 1 parts by weight of phosphoric acid hydrogen On the culture medium of dipotassium composition, culture, obtains lactic acid bacterial liquid;
Prepare saccharomycete bacterium solution:The cultured saccharomycete slant strains of picking are inoculated into by 1000 parts by weight water, 0.5 weight Measure part dusty yeast, 15 parts by weight glucose, seven water sulfuric acid of 3 parts sulfuric acid ammonia, 1 parts by weight of phosphoric acid potassium dihydrogen and 0.5 parts by weight On the culture medium of magnesium composition, culture, obtains saccharomycete bacterium solution;
Prepare bacillus subtilis and bacillus thuringiensis bacterium solution:The cultured bacillus subtilis of picking and Su Yunjin Bacillus slant strains are inoculated into by 1000 parts by weight water, 10 parts by weight peptones, 15 parts by weight glucose, 3 parts by weight chlorine On the culture medium for changing sodium and 1 parts by weight of phosphoric acid potassium dihydrogen composition, culture, obtains bacillus subtilis and bacillus thuringiensis bacterium Liquid;
Step 2.2:Prepare complex microbial community:First by fermentation cylinder for fermentation culture medium and fermentation tank in 121 DEG C of sterilizings 20min, then photosynthetic bacteria liquid, actinomyces bacterium solution, lactic acid bacterial liquid, saccharomycete bacterium solution, withered grass gemma will be obtained in step 2.1 Bacillus and bacillus thuringiensis bacterium solution are inoculated into fermentation cylinder for fermentation training according to percentage by weight for 0.5% inoculum concentration respectively Support, up to complex microbial community;
Step 3:Prepare charcoal base microbe soil conditioner:80 parts by weight charcoals are made using comminutor Grain, then 10 parts by weight complex microorganism bacterium solutions are sprayed into comminutor, make charcoal to complex microorganism bacterium solution with sprayer Fully absorption, while 2 parts by weight oxidized starch adhesives are added, up to charcoal base microbe soil conditioner.
In step 2.2 fermentation cylinder for fermentation culture medium by 1000 parts by weight water, 20 parts by weight Corn powder, 5 parts by weight bean powderes, 10 parts by weight glucose, 3 parts by weight of phosphoric acid hydrogen dipotassiums, 0.5 parts sulfuric acid magnesium and 0.1 weight parts of calcium carbonate composition.
Fermentation temperature is 35 DEG C in step 2.2, and fermentation pH controls are 6.0.
Embodiment 2
Charcoal base microbe soil conditioner, is made of following component:85 parts by weight charcoals, 13 parts by weight are compound micro- Biological flora and 1 parts by weight oxidized starch adhesive;
In the above-mentioned technical solutions, strain includes in complex microbial community:Photosynthetic bacteria, actinomyces, lactic acid bacteria, yeast Bacterium, bacillus subtilis and bacillus thuringiensis, the content of each strain are respectively:Photosynthetic bacteria content not less than 10,000,000,000/ Ml, unwrapping wire bacterial content not less than 15,000,000,000/ml, content of lactic acid bacteria not less than 15,000,000,000/ml, yeast bacterial content not less than 2,000,000,000/ Ml, bacillus subtilis bacterial content are not less than 2,000,000,000/ml and bacillus thuringiensis content is not less than 2,000,000,000/ml;
The viscosity of oxidized starch adhesive is 30mPa ﹒ s, and the oxidized starch adhesive is by 150 weight starch, 5 weights Measure part polyvinyl alcohol, 3 parts by weight glycerine, 10 parts by weight, 30% hydrogen peroxide, 2 parts by weight boraxs composition.
The method for preparing charcoal base microbe soil conditioner, includes the following steps:
Step 1:Prepare charcoal
Step 1.1:By stalk in air-drying processing under natural conditions, it is spare to be all milled to particle diameter 2mm with pulverizer, obtains Stalk;
Step 1.2:By the stalk after being ground in step 1.1, carbonizing apparatus hopper is sent into by conveyer belt, and utilize Raw material is sent to progress fertilizer separation in the separator on retort top by high pressure positive blower, and raw material puts live charcoal after entering retort Change, carbonization temperature is 500 DEG C, and heating rate is 5 DEG C/min, when charing 3 is small, up to charcoal;
Step 2:Prepare complex microbial community
Step 2.1:Prepare single bacterium solution:
Prepare photosynthetic bacteria liquid:The cultured photosynthetic bacteria slant strains of picking are inoculated into by 1000 parts by weight water, 5 Parts by weight yeast extract, 3 parts by weight acetic acid sodium, 1 parts by weight sal-ammoniac, 1 parts by weight of phosphoric acid potassium dihydrogen and 0.5 parts sulfuric acid magnesium On the culture medium of composition, culture, obtains photosynthetic bacteria liquid;
Prepare actinomyces bacterium solution:The cultured actinomyces slant strains of picking are inoculated into by 1000 parts by weight water, 20 weight Part soluble starch, 1 parts by weight, three water dipotassium hydrogen phosphate, 1 parts by wt NaCl, 1 parts by weight epsom salt, 1 parts by weight seven On the culture medium of aqueous ferrous sulfate and 2 parts by weight of potassium nitrate composition, culture, obtains actinomyces bacterium solution;
Prepare lactic acid bacterial liquid:The cultured lactic acid bacteria slant strains of picking are inoculated into by 1000 parts by weight water, 10 weight Part peptone, 10 parts by weight beef extracts, 5 parts by weight yeast extracts, 20 parts by weight glucose, 2 parts by weight of lemon acid diamines, 5 weight Part sodium acetate, 2 parts by weight Tween 80s, 0.5 parts by weight epsom salt, 0.3 parts by weight, four water manganese sulfate and 2 parts by weight of phosphoric acid hydrogen On the culture medium of dipotassium composition, culture, obtains lactic acid bacterial liquid;
Prepare saccharomycete bacterium solution:The cultured saccharomycete slant strains of picking are inoculated into by 1000 parts by weight water, 1 weight Part dusty yeast, 20 parts by weight glucose, 5 parts sulfuric acid ammonia, 2 parts by weight of phosphoric acid potassium dihydrogens and 1 parts by weight epsom salt group Into culture medium on, culture, obtain saccharomycete bacterium solution;
Prepare bacillus subtilis and bacillus thuringiensis bacterium solution:The cultured bacillus subtilis of picking and Su Yunjin Bacillus slant strains are inoculated into by 1000 parts by weight water, 15 parts by weight peptones, 20 parts by weight glucose, 5 parts by weight chlorine On the culture medium for changing sodium and 2 parts by weight of phosphoric acid potassium dihydrogens composition, culture, obtains bacillus subtilis and bacillus thuringiensis bacterium Liquid;
Step 2.2:Prepare complex microbial community:First by fermentation cylinder for fermentation culture medium and fermentation tank in 121 DEG C of sterilizings 20min, then photosynthetic bacteria liquid, actinomyces bacterium solution, lactic acid bacterial liquid, saccharomycete bacterium solution, withered grass gemma will be obtained in step 2.1 Bacillus and bacillus thuringiensis bacterium solution are inoculated into fermentation cylinder for fermentation culture according to percentage by weight for 1% inoculum concentration respectively, Up to complex microbial community;
Step 3:Prepare charcoal base microbe soil conditioner:85 parts by weight charcoals are made using comminutor Grain, then 13 parts by weight complex microorganism bacterium solutions are sprayed into comminutor, make charcoal to complex microorganism bacterium solution with sprayer Fully absorption, while 1 parts by weight oxidized starch adhesive is added, up to charcoal base microbe soil conditioner.
Fermentation cylinder for fermentation culture medium is by 1000 parts by weight water, 25 parts by weight Corn powder, 25 parts by weight beans in step 2.2 Powder, 15 parts by weight glucose, 5 parts by weight of phosphoric acid hydrogen dipotassiums, 1 parts sulfuric acid magnesium and 0.2 weight parts of calcium carbonate composition.
Fermentation temperature is 38 DEG C in step 2.2, and fermentation pH controls are 7.0.
Embodiment 3
Charcoal base microbe soil conditioner, is made of following component:83 parts by weight charcoals, 12 parts by weight are compound micro- Biological flora and 1.5 parts by weight oxidized starch adhesives;
In the above-mentioned technical solutions, strain includes in complex microbial community:Photosynthetic bacteria, actinomyces, lactic acid bacteria, yeast Bacterium, bacillus subtilis and bacillus thuringiensis, the content of each strain are respectively:Photosynthetic bacteria content not less than 10,000,000,000/ Ml, unwrapping wire bacterial content not less than 15,000,000,000/ml, content of lactic acid bacteria not less than 15,000,000,000/ml, yeast bacterial content not less than 2,000,000,000/ Ml, bacillus subtilis bacterial content are not less than 2,000,000,000/ml and bacillus thuringiensis content is not less than 2,000,000,000/ml;
The viscosity of oxidized starch adhesive is 28mPa ﹒ s, and the oxidized starch adhesive is by 120 weight starch, 4 weights Measure part polyvinyl alcohol, 2 parts by weight glycerine, 9 parts by weight, 30% hydrogen peroxide, 1.5 parts by weight boraxs composition.
The method for preparing charcoal base microbe soil conditioner, includes the following steps:
Step 1:Prepare charcoal
Step 1.1:By stalk in air-drying processing under natural conditions, it is spare to be all milled to particle diameter 2mm with pulverizer, obtains Stalk;
Step 1.2:By the stalk after being ground in step 1.1, carbonizing apparatus hopper is sent into by conveyer belt, and utilize Raw material is sent to progress fertilizer separation in the separator on retort top by high pressure positive blower, and raw material puts live charcoal after entering retort Change, carbonization temperature is 500 DEG C, and heating rate is 5 DEG C/min, when charing 28 is small, up to charcoal;
Step 2:Prepare complex microbial community
Step 2.1:Prepare single bacterium solution:
Prepare photosynthetic bacteria liquid:The cultured photosynthetic bacteria slant strains of picking are inoculated into by 1000 parts by weight water, 4 Parts by weight yeast extract, 2.5 parts by weight acetic acid sodium, 0.8 parts by weight sal-ammoniac, 0.7 parts by weight of phosphoric acid potassium dihydrogen and 0.4 parts by weight sulphur On the culture medium of sour magnesium composition, culture, obtains photosynthetic bacteria liquid;
Prepare actinomyces bacterium solution:The cultured actinomyces slant strains of picking are inoculated into by 1000 parts by weight water, 18 weight Part soluble starch, 0.8 parts by weight, three water dipotassium hydrogen phosphate, 0.8 parts by wt NaCl, 0.8 parts by weight epsom salt, 0.8 On the culture medium of parts by weight ferrous sulfate heptahydrate and 1.5 parts by weight of potassium nitrate composition, culture, obtains actinomyces bacterium solution;
Prepare lactic acid bacterial liquid:The cultured lactic acid bacteria slant strains of picking are inoculated into by 1000 parts by weight water, 8 weight Part peptone, 8 parts by weight beef extracts, 4 parts by weight yeast extracts, 18 parts by weight glucose, 1.5 parts by weight of lemon acid diamines, 4 weights Measure part sodium acetate, 1.5 parts by weight Tween 80s, 0.4 parts by weight epsom salt, 0.2 parts by weight, four water manganese sulfate and 1.5 parts by weight On the culture medium of dipotassium hydrogen phosphate composition, culture, obtains lactic acid bacterial liquid;
Prepare saccharomycete bacterium solution:The cultured saccharomycete slant strains of picking are inoculated into by 1000 parts by weight water, 0.8 weight Measure part dusty yeast, 18 parts by weight glucose, seven water sulphur of 4 parts sulfuric acid ammonia, 1.5 parts by weight of phosphoric acid potassium dihydrogens and 0.8 parts by weight On the culture medium of sour magnesium composition, culture, obtains saccharomycete bacterium solution;
Prepare bacillus subtilis and bacillus thuringiensis bacterium solution:The cultured bacillus subtilis of picking and Su Yunjin Bacillus slant strains are inoculated into by 1000 parts by weight water, 12 parts by weight peptones, 18 parts by weight glucose, 4 parts by weight chlorine On the culture medium for changing sodium and 1.5 parts by weight of phosphoric acid potassium dihydrogens composition, culture, obtains bacillus subtilis and bacillus thuringiensis Bacterium solution;
Step 2.2:Prepare complex microbial community:First by fermentation cylinder for fermentation culture medium and fermentation tank in 121 DEG C of sterilizings 20min, then photosynthetic bacteria liquid, actinomyces bacterium solution, lactic acid bacterial liquid, saccharomycete bacterium solution, withered grass gemma will be obtained in step 2.1 Bacillus and bacillus thuringiensis bacterium solution are inoculated into fermentation cylinder for fermentation training according to percentage by weight for 0.8% inoculum concentration respectively Support, up to complex microbial community;
Step 3:Prepare charcoal base microbe soil conditioner:83 parts by weight charcoals are made using comminutor Grain, then 12 parts by weight complex microorganism bacterium solutions are sprayed into comminutor, make charcoal to complex microorganism bacterium solution with sprayer Fully absorption, while 1.5 parts by weight oxidized starch adhesives are added, up to charcoal base microbe soil conditioner.
Fermentation cylinder for fermentation culture medium is by 1000 parts by weight water, 22 parts by weight Corn powder, 15 parts by weight beans in step 2.2 Powder, 12 parts by weight glucose, 4 parts by weight of phosphoric acid hydrogen dipotassiums, 0.8 parts sulfuric acid magnesium and 0.15 weight parts of calcium carbonate composition.
Fermentation temperature is 35~38 DEG C in step 2.2, and fermentation pH controls are 6.0.
Embodiment 4
Charcoal base microbe soil conditioner, is made of following component:82 parts by weight charcoals, 11 parts by weight are compound micro- Biological flora and 1 parts by weight oxidized starch adhesive;
Strain includes in complex microbial community:Photosynthetic bacteria, actinomyces, lactic acid bacteria, saccharomycete, bacillus subtilis and Bacillus thuringiensis, the content of each strain are respectively:Photosynthetic bacteria content is not less than not less than 10,000,000,000/ml, unwrapping wire bacterial content 15000000000/ml, content of lactic acid bacteria are not less than 15,000,000,000/ml, yeast bacterial content not less than 2,000,000,000/ml, bacillus subtilis bacterial content not It is not less than 2,000,000,000/ml less than 2,000,000,000/ml and bacillus thuringiensis content;
The viscosity of oxidized starch adhesive is 30mPa ﹒ s, and the oxidized starch adhesive is by 110 weight starch, 3 weights Measure part polyvinyl alcohol, 3 parts by weight glycerine, 9 parts by weight, 30% hydrogen peroxide, 1 parts by weight borax composition.
The method for preparing charcoal base microbe soil conditioner, includes the following steps:
Step 1:Prepare charcoal
Step 1.1:By stalk in air-drying processing under natural conditions, it is spare to be all milled to particle diameter 2mm with pulverizer, obtains Stalk;
Step 1.2:By the stalk after being ground in step 1.1, carbonizing apparatus hopper is sent into by conveyer belt, and utilize Raw material is sent to progress fertilizer separation in the separator on retort top by high pressure positive blower, and raw material puts live charcoal after entering retort Change, carbonization temperature is 500 DEG C, and heating rate is 5 DEG C/min, when charing 3 is small, up to charcoal;
Step 2:Prepare complex microbial community
Step 2.1:Prepare single bacterium solution:
Prepare photosynthetic bacteria liquid:The cultured photosynthetic bacteria slant strains of picking are inoculated into by 1000 parts by weight water, 4 weights Measure part yeast extract, 2 parts by weight acetic acid sodium, 1 parts by weight sal-ammoniac, 0.8 parts by weight of phosphoric acid potassium dihydrogen and 0.4 parts sulfuric acid magnesium On the culture medium of composition, culture, obtains photosynthetic bacteria liquid;
Prepare actinomyces bacterium solution:The cultured actinomyces slant strains of picking are inoculated into by 1000 parts by weight water, 18 weight Part soluble starch, 1 parts by weight, three water dipotassium hydrogen phosphate, 0.5 parts by wt NaCl, 0.8 parts by weight epsom salt, 0.5 weight On the culture medium for measuring part ferrous sulfate heptahydrate and 2 parts by weight of potassium nitrate composition, culture, obtains actinomyces bacterium solution;
Prepare lactic acid bacterial liquid:The cultured lactic acid bacteria slant strains of picking are inoculated into by 1000 parts by weight water, 8 weight Part peptone, 10 parts by weight beef extracts, 3 parts by weight yeast extracts, 15 parts by weight glucose, 2 parts by weight of lemon acid diamines, 3 weight Part sodium acetate, 1 parts by weight Tween 80,0.5 parts by weight epsom salt, 0.1 parts by weight, four water manganese sulfate and 1 parts by weight of phosphoric acid hydrogen On the culture medium of dipotassium composition, culture, obtains lactic acid bacterial liquid;
Prepare saccharomycete bacterium solution:The cultured saccharomycete slant strains of picking are inoculated into by 1000 parts by weight water, 0.8 weight Measure part dusty yeast, 18 parts by weight glucose, seven water sulfuric acid of 3 parts sulfuric acid ammonia, 2 parts by weight of phosphoric acid potassium dihydrogens and 0.8 parts by weight On the culture medium of magnesium composition, culture, obtains saccharomycete bacterium solution;
Prepare bacillus subtilis and bacillus thuringiensis bacterium solution:The cultured bacillus subtilis of picking and Su Yunjin Bacillus slant strains are inoculated into by 1000 parts by weight water, 13 parts by weight peptones, 16 parts by weight glucose, 4 parts by weight chlorine On the culture medium for changing sodium and 1 parts by weight of phosphoric acid potassium dihydrogen composition, culture, obtains bacillus subtilis and bacillus thuringiensis bacterium Liquid;
Step 2.2:Prepare complex microbial community:First by fermentation cylinder for fermentation culture medium and fermentation tank in 121 DEG C of sterilizings 20min, then photosynthetic bacteria liquid, actinomyces bacterium solution, lactic acid bacterial liquid, saccharomycete bacterium solution, withered grass gemma will be obtained in step 2.1 Bacillus and bacillus thuringiensis bacterium solution are inoculated into fermentation cylinder for fermentation culture according to percentage by weight for 1% inoculum concentration respectively, Up to complex microbial community;
Step 3:Prepare charcoal base microbe soil conditioner:82 parts by weight charcoals are made using comminutor Grain, then 11 parts by weight complex microorganism bacterium solutions are sprayed into comminutor, make charcoal to complex microorganism bacterium solution with sprayer Fully absorption, while 1 parts by weight oxidized starch adhesive is added, up to charcoal base microbe soil conditioner.
Fermentation cylinder for fermentation culture medium is by 1000 parts by weight water, 22 parts by weight Corn powder, 18 parts by weight beans in step 2.2 Powder, 14 parts by weight glucose, 4 parts by weight of phosphoric acid hydrogen dipotassiums, 0.8 parts sulfuric acid magnesium and 0.1 weight parts of calcium carbonate composition.
Fermentation temperature is 35 DEG C in step 2.2, and fermentation pH controls are 7.0.
Embodiment 5
Charcoal base microbe soil conditioner, is made of following component:85 parts by weight charcoals, 13 parts by weight are compound micro- Biological flora and 2 parts by weight oxidized starch adhesives;
Strain includes in complex microbial community:Photosynthetic bacteria, actinomyces, lactic acid bacteria, saccharomycete, bacillus subtilis and Bacillus thuringiensis, the content of each strain are respectively:Photosynthetic bacteria content is not less than not less than 10,000,000,000/ml, unwrapping wire bacterial content 15000000000/ml, content of lactic acid bacteria are not less than 15,000,000,000/ml, yeast bacterial content not less than 2,000,000,000/ml, bacillus subtilis bacterial content not It is not less than 2,000,000,000/ml less than 2,000,000,000/ml and bacillus thuringiensis content;
The viscosity of oxidized starch adhesive is 30mPa ﹒ s, and the oxidized starch adhesive is by 150 weight starch, 3.5 Weight account polyethylene alcohol, 2 parts by weight glycerine, 9 parts by weight, 30% hydrogen peroxide and 2 parts by weight boraxs composition.
The method for preparing charcoal base microbe soil conditioner, includes the following steps:
Step 1:Prepare charcoal
Step 1.1:By stalk in air-drying processing under natural conditions, it is spare to be all milled to particle diameter 2mm with pulverizer, obtains Stalk;
Step 1.2:By the stalk after being ground in step 1.1, carbonizing apparatus hopper is sent into by conveyer belt, and utilize Raw material is sent to progress fertilizer separation in the separator on retort top by high pressure positive blower, and raw material puts live charcoal after entering retort Change, carbonization temperature is 500 DEG C, and heating rate is 5 DEG C/min, when charing 3 is small, up to charcoal;
Step 2:Prepare complex microbial community
Step 2.1:Prepare single bacterium solution:
Prepare photosynthetic bacteria liquid:The cultured photosynthetic bacteria slant strains of picking be inoculated into by 1000 parts by weight water, 3.5 parts by weight yeast extracts, 2.5 parts by weight acetic acid sodium, 1.0 parts by weight sal-ammoniacs, 0.5 parts by weight of phosphoric acid potassium dihydrogen and 0.4 weight On the culture medium of part magnesium sulfate composition, culture, obtains photosynthetic bacteria liquid;
Prepare actinomyces bacterium solution:The cultured actinomyces slant strains of picking are inoculated into by 1000 parts by weight water, 20 weight Part soluble starch, 0.5 parts by weight, three water dipotassium hydrogen phosphate, 1.0 parts by wt NaCl, 0.5 parts by weight epsom salt, 0.8 On the culture medium of parts by weight ferrous sulfate heptahydrate and 1~2 parts by weight of potassium nitrate composition, culture, obtains actinomyces bacterium solution;
Prepare lactic acid bacterial liquid:The cultured lactic acid bacteria slant strains of picking are inoculated into by 1000 parts by weight water, 8.0 weights Measure part peptone, 10.0 parts by weight beef extracts, 5.0 parts by weight yeast extracts, 16.0 parts by weight glucose, 1.0 parts by weight of lemon acid Diamines, 3.5 parts by weight sodium acetates, 2.0 parts by weight Tween 80s, 0.4 parts by weight epsom salt, 0.2 parts by weight, four water manganese sulfate On the culture medium of 1~2 parts by weight of phosphoric acid hydrogen dipotassium composition, culture, obtains lactic acid bacterial liquid;
Prepare saccharomycete bacterium solution:The cultured saccharomycete slant strains of picking are inoculated into by 1000 parts by weight water, 0.5 weight Measure part dusty yeast, 20.0 parts by weight glucose, 5.0 parts sulfuric acid ammonia, 1.0 parts by weight of phosphoric acid potassium dihydrogens and 0.5 parts by weight seven On the culture medium of water magnesium sulfate composition, culture, obtains saccharomycete bacterium solution;
Prepare bacillus subtilis and bacillus thuringiensis bacterium solution:The cultured bacillus subtilis of picking and Su Yunjin Bacillus slant strains are inoculated into by 1000 parts by weight water, 15.0 parts by weight peptones, 20.0 parts by weight glucose, 5.0 weights On the culture medium for measuring part sodium chloride and 2.0 parts by weight of phosphoric acid potassium dihydrogens composition, culture, obtains bacillus subtilis and Su Yun gold buds Spore bacillus bacterium solution;
Step 2.2:Prepare complex microbial community:First by fermentation cylinder for fermentation culture medium and fermentation tank in 121 DEG C of sterilizings 20min, then photosynthetic bacteria liquid, actinomyces bacterium solution, lactic acid bacterial liquid, saccharomycete bacterium solution, withered grass gemma will be obtained in step 2.1 Bacillus and bacillus thuringiensis bacterium solution are inoculated into fermentation cylinder for fermentation training according to percentage by weight for 0.8% inoculum concentration respectively Support, up to complex microbial community;
Step 3:Prepare charcoal base microbe soil conditioner:85 parts by weight charcoals are made using comminutor Grain, then 13 parts by weight complex microorganism bacterium solutions are sprayed into comminutor, make charcoal to complex microorganism bacterium solution with sprayer Fully absorption, while 2 parts by weight oxidized starch adhesives are added, up to charcoal base microbe soil conditioner.
Fermentation cylinder for fermentation culture medium is by 1000 parts by weight water, 22.0 parts by weight Corn powder, 20.0 parts by weight in step 2.2 Bean powder, 15.0 parts by weight glucose, 3.0 parts by weight of phosphoric acid hydrogen dipotassiums, 0.5 parts sulfuric acid magnesium and 0.1 weight parts of calcium carbonate group Into.
Fermentation temperature is 35 DEG C in step 2.2, and fermentation pH controls are 7.0.
Benefit case
Case 1
Using pot experiment, the rice soil addition 5 charcoal base of the embodiment of the present invention for choosing progress Rice-rape rotation throughout the year is micro- Experiment of the biological soil conditioner to the soil weight, porosity and water content, experiment set following processing:
Processing one:5 charcoal base microbe soil conditioner (T1) of the embodiment of the present invention is not added;
Processing two:It is 1% that 5 charcoal base microbe soil conditioner of the embodiment of the present invention, which is added, with dry ground mass ratio (T2);
Processing three:It is 2% that 5 charcoal base microbe soil conditioner of the embodiment of the present invention, which is added, with dry ground mass ratio (T3);
Processing four:It is 4% that 5 charcoal base microbe soil conditioner of the embodiment of the present invention, which is added, with dry ground mass ratio (T4);
Designed using randomized block experiment, each processing sets 5 repetitions.
Experimental result is as shown in Figure 1:The soil weight the result shows that, when in soil add 5 charcoal base of the embodiment of the present invention When microbe soil conditioner and dry ground mass ratio are 1%, the unit weight of rice soil is 1.49gcm-3, relatively compare (1.55g cm-3) reduce by 3.87%;When addition 5 charcoal base microbe soil conditioner of the embodiment of the present invention in soil and dry ground mass ratio For 2% and 4% when, the unit weight of rice soil is 1.41gcm-3And 1.34gcm-39.03% and 13.55% is reduced compared with control. As can be seen that addition 5 charcoal base microbe soil conditioner of the embodiment of the present invention can reduce the soil weight, and with use The increase of ratio and reduce.
Soil porosity the result shows that:Adding 5 charcoal base microbe soil conditioner of the embodiment of the present invention in soil can To dramatically increase soil porosity, amplification is 20.97~53.46%;Soil moisture content the result shows that, in soil add the present invention 5 charcoal base microbe soil conditioner of embodiment can improve soil moisture content, and amplification is 22.50~54.34%.It can see Go out, addition 5 charcoal base microbe soil conditioner of the embodiment of the present invention adds soil permeability and retentiveness.
Case 2
In Jingmen City, Hubei Province Shayang County, once market town Rice-rape rotation area development low land power rapeseed cultivation field soil adds this hair Influence of the bright 5 charcoal base microbe soil conditioner of embodiment to soil nutrient and yield of rape, experiment set following processing:
Processing one:5 charcoal base microbe soil conditioner (T of the embodiment of the present invention is not added1);
Processing two:Add 5 charcoal base microbe soil conditioner 1thm of the embodiment of the present invention-2 (T2);
Processing three:Add 5 charcoal base microbe soil conditioner 5thm of the embodiment of the present invention-2 (T3);
Processing four:Add 5 charcoal base microbe soil conditioner 10thm of the embodiment of the present invention-2(T4);
Designed using randomized block experiment, each processing sets 3 repetitions.Per plot area 20m2
Phosphorus, potassium, boron fertilizer make base manure one-time use in experiment, nitrogenous fertilizer application in two dressings (60% base manure+20% get over winter top dressing+ 20% a kind of sedge fertilizer);Fertilizer type used is urea, calcium superphosphate, potassium chloride and borax.
Rape variety is Hua-you-za No.9;Planted using transplanting method.
Result of the test is as shown in Figure 2:Soil nutrient status as shown by data, 5 charcoal of the embodiment of the present invention is added in soil Base microbe soil conditioner can significantly improve content of soil nitrate-N, and amplification is 26.97~70.79%, and to soil ammonium The change of state nitrogen has no significant effect;Addition 5 charcoal base microbe soil conditioner of the embodiment of the present invention can significantly improve at the same time Soil organic matter content, amplification are 16.89~52.30%.5 charcoal base microbe soil conditioner of the embodiment of the present invention removes Mineral element contained by itself, its influence to soil nutrient mostly with charcoal base self character and microbiological effect Indirectly-acting is related.5 charcoal base microbe soil conditioner of the embodiment of the present invention can not only reduce the leaching loss of nutrient, effectively Promote the delay of nutrient in soil, played in earth soil biochemistry process particularly Nutrient Cycling very important Effect.
Yield result shows:After soil addition 5 charcoal base microbe soil conditioner of the embodiment of the present invention, soil physics Chemical-biological property is effectively improved, and builds a plant growth control environment, so as to pass through Soil-crop System shadow The growth to crop is rung, this experiment addition Biochar base soil modifier, yield of rape amplification is 8.55~24.19%.
Other parts not being described in detail are the prior art.

Claims (3)

1. charcoal base microbe soil conditioner, it is characterised in that:It is made of following component:80~85 parts by weight charcoals, 10~13 parts by weight complex microbial communities and 1~2 parts by weight oxidized starch adhesive;
The complex microbial community is made of following strain:Photosynthetic bacteria, actinomyces, lactic acid bacteria, saccharomycete, bacillus subtilis Bacterium and bacillus thuringiensis, the content of each strain are:Photosynthetic bacteria content is not less than not less than 10,000,000,000/ml, unwrapping wire bacterial content 15000000000/ml, content of lactic acid bacteria are not less than 15,000,000,000/ml, yeast bacterial content not less than 2,000,000,000/ml, bacillus subtilis bacterial content not It is not less than 2,000,000,000/ml less than 2,000,000,000/ml and bacillus thuringiensis content;
The viscosity of the oxidized starch adhesive is 25~30mPa ﹒ s, and the oxidized starch adhesive is by 100~150 parts by weight Starch, 3~5 weight account polyethylene alcohol, 1~3 parts by weight glycerine, 8~10 parts by weight, 30% hydrogen peroxide and 1~2 parts by weight boron Sand forms;
The method for preparing the charcoal base microbe soil conditioner, includes the following steps:
Step 1:Prepare charcoal
Step 1.1:By stalk in air-drying processing under natural conditions, it is spare to be all milled to particle diameter 2mm with pulverizer, obtains stalk Raw material;
Step 1.2:By the stalk after being ground in step 1.1, carbonizing apparatus hopper is sent into by conveyer belt, and utilize high pressure Raw material is sent to progress fertilizer separation in the separator on retort top by wind turbine, and raw material enters igniting charing after retort, charcoal It is 500 DEG C to change temperature, and heating rate is 5 DEG C/min, when charing 2.5~3 is small, up to charcoal;
Step 2:Prepare complex microbial community
Step 2.1:Prepare single bacterium solution:
Prepare photosynthetic bacteria liquid:The cultured photosynthetic bacteria slant strains of picking are inoculated into by 1000 parts by weight water, 3~5 weights Measure part yeast extract, 2~3 parts by weight acetic acid sodium, 0.5~1 parts by weight sal-ammoniac, 0.5~1 parts by weight of phosphoric acid potassium dihydrogen and 0.3~ On the culture medium of 0.5 parts sulfuric acid magnesium composition, culture, obtains photosynthetic bacteria liquid;
Prepare actinomyces bacterium solution:The cultured actinomyces slant strains of picking are inoculated into by 1000 parts by weight water, 15~20 weight Part soluble starch, 0.5~1 parts by weight, three water dipotassium hydrogen phosphate, 0.5~1 parts by wt NaCl, 0.5~1 parts by weight, seven water sulphur On the culture medium of sour magnesium, 0.5~1 parts by weight ferrous sulfate heptahydrate and 1~2 parts by weight of potassium nitrate composition, culture, obtains actinomyces Bacterium solution;
Prepare lactic acid bacterial liquid:The cultured lactic acid bacteria slant strains of picking are inoculated into by 1000 parts by weight water, 5~10 parts by weight Peptone, 5~10 parts by weight beef extracts, 3~5 parts by weight yeast extracts, 15~20 parts by weight glucose, 1~2 parts by weight of lemon acid Diamines, 3~5 parts by weight sodium acetates, 1~2 parts by weight Tween 80,0.3~0.5 parts by weight epsom salt, 0.1~0.3 weight On the culture medium of four water manganese sulfates of part and 1~2 parts by weight of phosphoric acid hydrogen dipotassium composition, culture, obtains lactic acid bacterial liquid;
Prepare saccharomycete bacterium solution:The cultured saccharomycete slant strains of picking are inoculated into by 1000 parts by weight water, 0.5~1 weight Part dusty yeast, 15~20 parts by weight glucose, 3~5 parts sulfuric acid ammonia, 1~2 parts by weight of phosphoric acid potassium dihydrogen and 0.5~1 weight On the culture medium of part epsom salt composition, culture, obtains saccharomycete bacterium solution;
Prepare bacillus subtilis and bacillus thuringiensis bacterium solution:The cultured bacillus subtilis of picking and Su Yun gold gemma Bacillus slant strains are inoculated into by 1000 parts by weight water, 10~15 parts by weight peptones, 15~20 parts by weight glucose, 3~5 weights On the culture medium for measuring part sodium chloride and 1~2 parts by weight of phosphoric acid potassium dihydrogen composition, culture, obtains bacillus subtilis and Su Yunjin Bacillus bacterium solution;
Step 2.2:Prepare complex microbial community:First by fermentation cylinder for fermentation culture medium and fermentation tank in 121 DEG C of sterilizings 20min, then photosynthetic bacteria liquid, actinomyces bacterium solution, lactic acid bacterial liquid, saccharomycete bacterium solution, withered grass gemma will be obtained in step 2.1 Bacillus and bacillus thuringiensis bacterium solution are inoculated into fermentation tank according to percentage by weight for 0.5%~1% inoculum concentration respectively to be sent out Ferment culture, up to complex microbial community;
Step 3:Prepare charcoal base microbe soil conditioner:80~85 parts by weight charcoals are made using comminutor Grain, then 10~13 parts by weight complex microorganism bacterium solutions are sprayed into comminutor, make charcoal to complex microorganism with sprayer Bacterium solution is fully adsorbed, while adds 1~2 parts by weight oxidized starch adhesive, up to charcoal base microbe soil conditioner;
Fermentation cylinder for fermentation culture medium is by 1000 parts by weight water, 20~25 parts by weight Corn powder, 5~25 parts by weight in step 2.2 Bean powder, 10~15 parts by weight glucose, 3~5 parts by weight of phosphoric acid hydrogen dipotassiums, 0.5~1 parts sulfuric acid magnesium and 0.1~0.2 weight Part calcium carbonate composition;
Fermentation temperature is 35~38 DEG C in step 2.2, and fermentation pH controls are 6.0~7.0.
2. according to the charcoal base microbe soil conditioner described in claim 1, it is characterised in that be made of following component: 85 parts by weight charcoals, 13 parts by weight complex microbial communities and 2 parts by weight oxidized starch adhesives;
The viscosity of the oxidized starch adhesive is 30mPa ﹒ s, and the oxidized starch adhesive is by 150 weight starch, 3.5 weights Measure part polyvinyl alcohol, 2 parts by weight glycerine, 9 parts by weight, 30% hydrogen peroxide and 2 parts by weight boraxs composition.
3. the charcoal base microbe soil conditioner according to claim 2, it is characterised in that:Sent out in the step 2.2 Fermentation medium is by 1000 parts by weight water, 22 parts by weight Corn powder, 20 parts by weight bean powderes, 15 parts by weight glucose, 3 weights in fermentation tank Measure part dipotassium hydrogen phosphate, 0.5 parts sulfuric acid magnesium and 0.1 weight parts of calcium carbonate composition.
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